                         SEQUENCE LISTING

<110>  Advaxis, Inc.
       Petit, Robert
       Perry, Kyle
 
<120>  RECOMBINANT LISTERIA VACCINE STRAINS AND METHODS OF USING THE 
       SAME IN CANCER IMMUNOTHERAPY

<130>  062384/486701

<150>  US 62/241,712
<151>  2015-10-14

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  PRT
<213>  Listeria monocytogenes

<400>  1

Lys Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala 
1               5                   10                  15      


Ser Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys 
            20                  25                  30          


<210>  2
<211>  441
<212>  PRT
<213>  Listeria monocytogenes

<400>  2

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Val Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Ser Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp 
        435                 440     


<210>  3
<211>  529
<212>  PRT
<213>  Listeria monocytogenes

<400>  3

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp Pro Glu Gly Asn Glu Ile Val 
        435                 440                 445             


Gln His Lys Asn Trp Ser Glu Asn Asn Lys Ser Lys Leu Ala His Phe 
    450                 455                 460                 


Thr Ser Ser Ile Tyr Leu Pro Gly Asn Ala Arg Asn Ile Asn Val Tyr 
465                 470                 475                 480 


Ala Lys Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Thr Val Ile 
                485                 490                 495     


Asp Asp Arg Asn Leu Pro Leu Val Lys Asn Arg Asn Ile Ser Ile Trp 
            500                 505                 510         


Gly Thr Thr Leu Tyr Pro Lys Tyr Ser Asn Lys Val Asp Asn Pro Ile 
        515                 520                 525             


Glu 
    


<210>  4
<211>  416
<212>  PRT
<213>  Listeria monocytogenes

<400>  4

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Val Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Ser Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


<210>  5
<211>  390
<212>  PRT
<213>  Listeria monocytogenes

<400>  5

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys Val Arg Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Glu Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu Asn Ala Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Val Asn Lys Lys Lys Val Ala Lys Glu Ser Val Ala 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln Gln Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn Ala Ile Asn Arg His Ser 
        355                 360                 365             


Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro 
385                 390 


<210>  6
<211>  1170
<212>  DNA
<213>  Listeria monocytogenes

<400>  6
atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacata       60

atatttgcag cgacagatag cgaagattct agtctaaaca cagatgaatg ggaagaagaa      120

aaaacagaag agcaaccaag cgaggtaaat acgggaccaa gatacgaaac tgcacgtgaa      180

gtaagttcac gtgatattaa agaactagaa aaatcgaata aagtgagaaa tacgaacaaa      240

gcagacctaa tagcaatgtt gaaagaaaaa gcagaaaaag gtccaaatat caataataac      300

aacagtgaac aaactgagaa tgcggctata aatgaagagg cttcaggagc cgaccgacca      360

gctatacaag tggagcgtcg tcatccagga ttgccatcgg atagcgcagc ggaaattaaa      420

aaaagaagga aagccatagc atcatcggat agtgagcttg aaagccttac ttatccggat      480

aaaccaacaa aagtaaataa gaaaaaagtg gcgaaagagt cagttgcgga tgcttctgaa      540

agtgacttag attctagcat gcagtcagca gatgagtctt caccacaacc tttaaaagca      600

aaccaacaac catttttccc taaagtattt aaaaaaataa aagatgcggg gaaatgggta      660

cgtgataaaa tcgacgaaaa tcctgaagta aagaaagcga ttgttgataa aagtgcaggg      720

ttaattgacc aattattaac caaaaagaaa agtgaagagg taaatgcttc ggacttcccg      780

ccaccaccta cggatgaaga gttaagactt gctttgccag agacaccaat gcttcttggt      840

tttaatgctc ctgctacatc agaaccgagc tcattcgaat ttccaccacc acctacggat      900

gaagagttaa gacttgcttt gccagagacg ccaatgcttc ttggttttaa tgctcctgct      960

acatcggaac cgagctcgtt cgaatttcca ccgcctccaa cagaagatga actagaaatc     1020

atccgggaaa cagcatcctc gctagattct agttttacaa gaggggattt agctagtttg     1080

agaaatgcta ttaatcgcca tagtcaaaat ttctctgatt tcccaccaat cccaacagaa     1140

gaagagttga acgggagagg cggtagacca                                      1170


<210>  7
<211>  14
<212>  PRT
<213>  Listeria monocytogenes

<400>  7

Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg 
1               5                   10                  


<210>  8
<211>  28
<212>  PRT
<213>  Listeria monocytogenes

<400>  8

Lys Ala Ser Val Thr Asp Thr Ser Glu Gly Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  9
<211>  20
<212>  PRT
<213>  Listeria monocytogenes

<400>  9

Lys Asn Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  10
<211>  33
<212>  PRT
<213>  Listeria monocytogenes

<400>  10

Arg Gly Gly Ile Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  11
<211>  17
<212>  PRT
<213>  Streptococcus pyogenes

<400>  11

Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  12
<211>  17
<212>  PRT
<213>  Streptococcus equisimilis

<400>  12

Lys Gln Asn Thr Ala Asn Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  13
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E7 protein

<400>  13

Met His Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln 
1               5                   10                  15      


Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser 
            20                  25                  30          


Glu Glu Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp 
        35                  40                  45              


Arg Ala His Tyr Asn Ile Val Thr Phe Cys Cys Lys Cys Asp Ser Thr 
    50                  55                  60                  


Leu Arg Leu Cys Val Gln Ser Thr His Val Asp Ile Arg Thr Leu Glu 
65                  70                  75                  80  


Asp Leu Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys Ser Gln 
                85                  90                  95      


Lys Pro 
        


<210>  14
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E7 protein

<400>  14

Met His Gly Pro Lys Ala Thr Leu Gln Asp Ile Val Leu His Leu Glu 
1               5                   10                  15      


Pro Gln Asn Glu Ile Pro Val Asp Leu Leu Cys His Glu Gln Leu Ser 
            20                  25                  30          


Asp Ser Glu Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His 
        35                  40                  45              


Leu Pro Ala Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met 
    50                  55                  60                  


Cys Cys Lys Cys Glu Ala Arg Ile Glu Leu Val Val Glu Ser Ser Ala 
65                  70                  75                  80  


Asp Asp Leu Arg Ala Phe Gln Gln Leu Phe Leu Asn Thr Leu Ser Phe 
                85                  90                  95      


Val Cys Pro Trp Cys Ala Ser Gln Gln 
            100                 105 


<210>  15
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E6 protein

<400>  15

Met His Gln Lys Arg Thr Ala Met Phe Gln Asp Pro Gln Glu Arg Pro 
1               5                   10                  15      


Arg Lys Leu Pro Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile His Asp 
            20                  25                  30          


Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln Leu Leu Arg Arg Glu 
        35                  40                  45              


Val Tyr Asp Phe Ala Phe Arg Asp Leu Cys Ile Val Tyr Arg Asp Gly 
    50                  55                  60                  


Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys Ile 
65                  70                  75                  80  


Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu Glu 
                85                  90                  95      


Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile Asn 
            100                 105                 110         


Cys Gln Lys Pro Leu Cys Pro Glu Glu Lys Gln Arg His Leu Asp Lys 
        115                 120                 125             


Lys Gln Arg Phe His Asn Ile Arg Gly Arg Trp Thr Gly Arg Cys Met 
    130                 135                 140                 


Ser Cys Cys Arg Ser Ser Arg Thr Arg Arg Glu Thr Gln Leu 
145                 150                 155             


<210>  16
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E6 protein

<400>  16

Met Ala Arg Phe Glu Asp Pro Thr Arg Arg Pro Tyr Lys Leu Pro Asp 
1               5                   10                  15      


Leu Cys Thr Glu Leu Asn Thr Ser Leu Gln Asp Ile Glu Ile Thr Cys 
            20                  25                  30          


Val Tyr Cys Lys Thr Val Leu Glu Leu Thr Glu Val Phe Glu Phe Ala 
        35                  40                  45              


Phe Lys Asp Leu Phe Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala 
    50                  55                  60                  


Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His 
65                  70                  75                  80  


Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr 
                85                  90                  95      


Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Cys Gln Lys Pro Leu 
            100                 105                 110         


Asn Pro Ala Glu Lys Leu Arg His Leu Asn Glu Lys Arg Arg Phe His 
        115                 120                 125             


Asn Ile Ala Gly His Tyr Arg Gly Gln Cys His Ser Cys Cys Asn Arg 
    130                 135                 140                 


Ala Arg Gln Glu Arg Leu Gln Arg Arg Arg Glu Thr Gln Val 
145                 150                 155             


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
ggctcgagca tggagataca cc                                                22


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
ggggactagt ttatggtttc tgagaaca                                          28


<210>  19
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
gggggctagc cctcctttga ttagtatatt c                                      31


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
ctccctcgag atcataattt acttcatc                                          28


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
cccgtcgacc agctcttctt ggtgaag                                           27


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
gcggatccca tggagataca cctac                                             25


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
gctctagatt atggtttctg ag                                                22


<210>  24
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E7 peptide

<400>  24

Arg Ala His Tyr Asn Ile Val Thr Phe 
1               5                   


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytotoxic T-cell (CTL) epitope for HIV-GAG

<400>  25

Ala Met Gln Met Leu Lys Glu Thr Ile 
1               5                   


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytotoxic T-cell (CTL) epitope for Listeria LLO

<400>  26

Gly Tyr Lys Asp Gly Asn Glu Tyr Ile 
1               5                   


<210>  27
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
gactacaagg acgatgaccg acaagtgata acccgggatc taaataaatc cgttt            55


<210>  28
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
cctagctaaa tttaatgt                                                     18


<210>  29
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
cctagctaaa tttaatga                                                     18


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
taattttccc caagtagcag g                                                 21


<210>  31
<211>  1614
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding four heterologous antigen 
       sequences operably linked to a tag sequence

<400>  31
atgcatggag atacacctac attgcatgaa tatatgttag atttgcaacc agagacaact       60

gatctctact gttatgaaca attaaatgac agctcagagg aggaggatga aatagatggt      120

ccagctggac aagcagaacc ggacagagcc cattacaata ttgtaacctt ttgttgcaag      180

tgtgactcta cgcttcggtt gtgcgtacaa agcacacacg tagacattcg tactttggaa      240

gacctgttaa tgggcacact aggaattgtg tgccccatct gttctcagaa accaatgcat      300

caaaaacgta cagcaatgtt tcaggaccca caggagcgac ccagaaagtt accacagtta      360

tgcacagagc tgcaaacaac tatacatgat ataatattag aatgtgtgta ctgcaagcaa      420

cagttactgc gacgtgaggt atatgacttt gcttttcggg atttatgcat agtatataga      480

gatgggaatc catatgctgt atgtgataaa tgtttaaagt tttattctaa aattagtgag      540

tatagacatt attgttatag tttatatgga acaacattag aacagcaata caacaaaccg      600

ttgtgtgatt tgttaattag gtgtattaac tgtcaaaagc cactgtgtcc tgaagaaaag      660

caaagacatc tggacaaaaa gcaaagattc cataatataa ggggtcggtg gaccggtcga      720

tgtatgtctt gttgcagatc atcaagaaca cgtagagaaa cccagctgat gcatggacct      780

aaggcaacat tgcaagacat tgtattgcat ttagagcccc aaaatgaaat tccggttgac      840

cttctatgtc acgaacaatt aagcgactca gaggaagaaa acgatgaaat agatggagtt      900

aatcatcaac atttaccagc ccgacgagcc gaaccacaac gtcacacaat gttgtgtatg      960

tgttgtaagt gtgaagccag aattgagcta gtagtagaaa gctcagcaga cgaccttcga     1020

gcattccagc agctgtttct gaacaccctg tcctttgtgt gtccgtggtg tgcatcccag     1080

cagatggcgc gctttgagga tccaacacgg cgaccctaca agctacctga tctgtgcacg     1140

gaactgaaca cttcactgca agacatagaa ataacctgtg tatattgcaa gacagtattg     1200

gaacttacag aggtatttga atttgcattt aaagatttat ttgtggtgta tagagacagt     1260

ataccgcatg ctgcatgtca taaatgtatc gatttttatt caagaattag agaattaaga     1320

cattattcag actctgtgta tggagacaca ttggaaaaac taactaacac tgggttatac     1380

aatttattaa taaggtgcct gcggtgtcag aaaccgttga atccagcaga aaaacttaga     1440

caccttaatg aaaaacgacg atttcacaac atagctgggc actatagagg ccagtgccat     1500

tcgtgctgca accgagcacg acaggaacga ctccaacgac gcagagaaac acaagtagca     1560

cgttctatta ttaacttcga aaaattatct catcatcatc atcatcatta ataa           1614


<210>  32
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant polypeptide comprising four heterologous antigen 
       sequences operably linked at the N-terminus to truncated LLO and 
       a peptide tag sequence at the C-terminus

<400>  32
atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa       60

caaactgaag caaaggatgc atctgcattc aataaagaaa attcaatttc atccatggca      120

ccaccagcat ctccgcctgc aagtcctaag acgccaatcg aaaagaaaca cgcggatgaa      180

atcgataagt atatacaagg attggattac aataaaaaca atgtattagt ataccacgga      240

gatgcagtga caaatgtgcc gccaagaaaa ggttacaaag atggaaatga atatattgtt      300

gtggagaaaa agaagaaatc catcaatcaa aataatgcag acattcaagt tgtgaatgca      360

atttcgagcc taacctatcc aggtgctctc gtaaaagcga attcggaatt agtagaaaat      420

caaccagatg ttctccctgt aaaacgtgat tcattaacac tcagcattga tttgccaggt      480

atgactaatc aagacaataa aatagttgta aaaaatgcca ctaaatcaaa cgttaacaac      540

gcagtaaata cattagtgga aagatggaat gaaaaatatg ctcaagctta tccaaatgta      600

agtgcaaaaa ttgattatga tgacgaaatg gcttacagtg aatcacaatt aattgcgaaa      660

tttggtacag catttaaagc tgtaaataat agcttgaatg taaacttcgg cgcaatcagt      720

gaagggaaaa tgcaagaaga agtcattagt tttaaacaaa tttactataa cgtgaatgtt      780

aatgaaccta caagaccttc cagatttttc ggcaaagctg ttactaaaga gcagttgcaa      840

gcgcttggag tgaatgcaga aaatcctcct gcatatatct caagtgtggc gtatggccgt      900

caagtttatt tgaaattatc aactaattcc catagtacta aagtaaaagc tgcttttgat      960

gctgccgtaa gcggaaaatc tgtctcaggt gatgtagaac taacaaatat catcaaaaat     1020

tcttccttca aagccgtaat ttacggaggt tccgcaaaag atgaagttca aatcatcgac     1080

ggcaacctcg gagacttacg cgatattttg aaaaaaggcg ctacttttaa tcgagaaaca     1140

ccaggagttc ccattgctta tacaacaaac ttcctaaaag acaatgaatt agctgttatt     1200

aaaaacaact cagaatatat tgaaacaact tcaaaagctt atacagatgg aaaaattaac     1260

atcgatcact ctggaggata cgttgctcaa ttcaacattt cttgggatga agtaaattat     1320

gatctcgaga tgcatggaga tacacctaca ttgcatgaat atatgttaga tttgcaacca     1380

gagacaactg atctctactg ttatgaacaa ttaaatgaca gctcagagga ggaggatgaa     1440

atagatggtc cagctggaca agcagaaccg gacagagccc attacaatat tgtaaccttt     1500

tgttgcaagt gtgactctac gcttcggttg tgcgtacaaa gcacacacgt agacattcgt     1560

actttggaag acctgttaat gggcacacta ggaattgtgt gccccatctg ttctcagaaa     1620

ccaatgcatc aaaaacgtac agcaatgttt caggacccac aggagcgacc cagaaagtta     1680

ccacagttat gcacagagct gcaaacaact atacatgata taatattaga atgtgtgtac     1740

tgcaagcaac agttactgcg acgtgaggta tatgactttg cttttcggga tttatgcata     1800

gtatatagag atgggaatcc atatgctgta tgtgataaat gtttaaagtt ttattctaaa     1860

attagtgagt atagacatta ttgttatagt ttatatggaa caacattaga acagcaatac     1920

aacaaaccgt tgtgtgattt gttaattagg tgtattaact gtcaaaagcc actgtgtcct     1980

gaagaaaagc aaagacatct ggacaaaaag caaagattcc ataatataag gggtcggtgg     2040

accggtcgat gtatgtcttg ttgcagatca tcaagaacac gtagagaaac ccagctgatg     2100

catggaccta aggcaacatt gcaagacatt gtattgcatt tagagcccca aaatgaaatt     2160

ccggttgacc ttctatgtca cgaacaatta agcgactcag aggaagaaaa cgatgaaata     2220

gatggagtta atcatcaaca tttaccagcc cgacgagccg aaccacaacg tcacacaatg     2280

ttgtgtatgt gttgtaagtg tgaagccaga attgagctag tagtagaaag ctcagcagac     2340

gaccttcgag cattccagca gctgtttctg aacaccctgt cctttgtgtg tccgtggtgt     2400

gcatcccagc agatggcgcg ctttgaggat ccaacacggc gaccctacaa gctacctgat     2460

ctgtgcacgg aactgaacac ttcactgcaa gacatagaaa taacctgtgt atattgcaag     2520

acagtattgg aacttacaga ggtatttgaa tttgcattta aagatttatt tgtggtgtat     2580

agagacagta taccgcatgc tgcatgtcat aaatgtatcg atttttattc aagaattaga     2640

gaattaagac attattcaga ctctgtgtat ggagacacat tggaaaaact aactaacact     2700

gggttataca atttattaat aaggtgcctg cggtgtcaga aaccgttgaa tccagcagaa     2760

aaacttagac accttaatga aaaacgacga tttcacaaca tagctgggca ctatagaggc     2820

cagtgccatt cgtgctgcaa ccgagcacga caggaacgac tccaacgacg cagagaaaca     2880

caagtagcac gttctattat taacttcgaa aaattatctc atcatcatca tcatcattaa     2940

taa                                                                   2943


<210>  33
<211>  979
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant polypeptide tLLO-HPV16E7-HPV16E6-HPV18E7-HPV16E6 
       SIINFEKL-S-6xHIS tag

<400>  33

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp Leu Glu Met His Gly Asp Thr 
        435                 440                 445             


Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp 
    450                 455                 460                 


Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser Glu Glu Glu Asp Glu 
465                 470                 475                 480 


Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn 
                485                 490                 495     


Ile Val Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val 
            500                 505                 510         


Gln Ser Thr His Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly 
        515                 520                 525             


Thr Leu Gly Ile Val Cys Pro Ile Cys Ser Gln Lys Pro Met His Gln 
    530                 535                 540                 


Lys Arg Thr Ala Met Phe Gln Asp Pro Gln Glu Arg Pro Arg Lys Leu 
545                 550                 555                 560 


Pro Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile His Asp Ile Ile Leu 
                565                 570                 575     


Glu Cys Val Tyr Cys Lys Gln Gln Leu Leu Arg Arg Glu Val Tyr Asp 
            580                 585                 590         


Phe Ala Phe Arg Asp Leu Cys Ile Val Tyr Arg Asp Gly Asn Pro Tyr 
        595                 600                 605             


Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys Ile Ser Glu Tyr 
    610                 615                 620                 


Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu Glu Gln Gln Tyr 
625                 630                 635                 640 


Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile Asn Cys Gln Lys 
                645                 650                 655     


Pro Leu Cys Pro Glu Glu Lys Gln Arg His Leu Asp Lys Lys Gln Arg 
            660                 665                 670         


Phe His Asn Ile Arg Gly Arg Trp Thr Gly Arg Cys Met Ser Cys Cys 
        675                 680                 685             


Arg Ser Ser Arg Thr Arg Arg Glu Thr Gln Leu Met His Gly Pro Lys 
    690                 695                 700                 


Ala Thr Leu Gln Asp Ile Val Leu His Leu Glu Pro Gln Asn Glu Ile 
705                 710                 715                 720 


Pro Val Asp Leu Leu Cys His Glu Gln Leu Ser Asp Ser Glu Glu Glu 
                725                 730                 735     


Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro Ala Arg Arg 
            740                 745                 750         


Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys Lys Cys Glu 
        755                 760                 765             


Ala Arg Ile Glu Leu Val Val Glu Ser Ser Ala Asp Asp Leu Arg Ala 
    770                 775                 780                 


Phe Gln Gln Leu Phe Leu Asn Thr Leu Ser Phe Val Cys Pro Trp Cys 
785                 790                 795                 800 


Ala Ser Gln Gln Met Ala Arg Phe Glu Asp Pro Thr Arg Arg Pro Tyr 
                805                 810                 815     


Lys Leu Pro Asp Leu Cys Thr Glu Leu Asn Thr Ser Leu Gln Asp Ile 
            820                 825                 830         


Glu Ile Thr Cys Val Tyr Cys Lys Thr Val Leu Glu Leu Thr Glu Val 
        835                 840                 845             


Phe Glu Phe Ala Phe Lys Asp Leu Phe Val Val Tyr Arg Asp Ser Ile 
    850                 855                 860                 


Pro His Ala Ala Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg 
865                 870                 875                 880 


Glu Leu Arg His Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys 
                885                 890                 895     


Leu Thr Asn Thr Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Cys 
            900                 905                 910         


Gln Lys Pro Leu Asn Pro Ala Glu Lys Leu Arg His Leu Asn Glu Lys 
        915                 920                 925             


Arg Arg Phe His Asn Ile Ala Gly His Tyr Arg Gly Gln Cys His Ser 
    930                 935                 940                 


Cys Cys Asn Arg Ala Arg Gln Glu Arg Leu Gln Arg Arg Arg Glu Thr 
945                 950                 955                 960 


Gln Val Ala Arg Ser Ile Ile Asn Phe Glu Lys Leu Ser His His His 
                965                 970                 975     


His His His 
            


<210>  34
<211>  6523
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pAdv142

<400>  34
cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg       60

tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc      120

cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc      180

ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag      240

agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc      300

tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      360

cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg      420

ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg      480

ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg      540

taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac      600

tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa      660

ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag      720

ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga      780

ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct      840

agccctcctt tgattagtat attcctatct taaagttact tttatgtgga ggcattaaca      900

tttgttaatg acgtcaaaag gatagcaaga ctagaataaa gctataaagc aagcatataa      960

tattgcgttt catctttaga agcgaatttc gccaatatta taattatcaa aagagagggg     1020

tggcaaacgg tatttggcat tattaggtta aaaaatgtag aaggagagtg aaacccatga     1080

aaaaaataat gctagttttt attacactta tattagttag tctaccaatt gcgcaacaaa     1140

ctgaagcaaa ggatgcatct gcattcaata aagaaaattc aatttcatcc atggcaccac     1200

cagcatctcc gcctgcaagt cctaagacgc caatcgaaaa gaaacacgcg gatgaaatcg     1260

ataagtatat acaaggattg gattacaata aaaacaatgt attagtatac cacggagatg     1320

cagtgacaaa tgtgccgcca agaaaaggtt acaaagatgg aaatgaatat attgttgtgg     1380

agaaaaagaa gaaatccatc aatcaaaata atgcagacat tcaagttgtg aatgcaattt     1440

cgagcctaac ctatccaggt gctctcgtaa aagcgaattc ggaattagta gaaaatcaac     1500

cagatgttct ccctgtaaaa cgtgattcat taacactcag cattgatttg ccaggtatga     1560

ctaatcaaga caataaaata gttgtaaaaa atgccactaa atcaaacgtt aacaacgcag     1620

taaatacatt agtggaaaga tggaatgaaa aatatgctca agcttatcca aatgtaagtg     1680

caaaaattga ttatgatgac gaaatggctt acagtgaatc acaattaatt gcgaaatttg     1740

gtacagcatt taaagctgta aataatagct tgaatgtaaa cttcggcgca atcagtgaag     1800

ggaaaatgca agaagaagtc attagtttta aacaaattta ctataacgtg aatgttaatg     1860

aacctacaag accttccaga tttttcggca aagctgttac taaagagcag ttgcaagcgc     1920

ttggagtgaa tgcagaaaat cctcctgcat atatctcaag tgtggcgtat ggccgtcaag     1980

tttatttgaa attatcaact aattcccata gtactaaagt aaaagctgct tttgatgctg     2040

ccgtaagcgg aaaatctgtc tcaggtgatg tagaactaac aaatatcatc aaaaattctt     2100

ccttcaaagc cgtaatttac ggaggttccg caaaagatga agttcaaatc atcgacggca     2160

acctcggaga cttacgcgat attttgaaaa aaggcgctac ttttaatcga gaaacaccag     2220

gagttcccat tgcttataca acaaacttcc taaaagacaa tgaattagct gttattaaaa     2280

acaactcaga atatattgaa acaacttcaa aagcttatac agatggaaaa attaacatcg     2340

atcactctgg aggatacgtt gctcaattca acatttcttg ggatgaagta aattatgatc     2400

tcgagattgt gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg     2460

cctctcgtgg cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag     2520

ctgcccactg catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc     2580

ctgaagacac aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata     2640

tgagcctcct gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc     2700

tgctccgcct gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca     2760

cccaggagcc agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag     2820

aggagttctt gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg     2880

tgtgtgcgca agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga     2940

cagggggcaa aagcacctgc tcgggtgatt ctgggggccc acttgtctgt tatggtgtgc     3000

ttcaaggtat cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt     3060

acaccaaggt ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctaac     3120

ccgggccact aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga     3180

accagaaaca gaacaagtaa cattggagtt agaaatggaa gaagaaaaaa gcaatgattt     3240

cgtgtgaata atgcacgaaa tcattgctta tttttttaaa aagcgatata ctagatataa     3300

cgaaacaacg aactgaataa agaatacaaa aaaagagcca cgaccagtta aagcctgaga     3360

aactttaact gcgagcctta attgattacc accaatcaat taaagaagtc gagacccaaa     3420

atttggtaaa gtatttaatt actttattaa tcagatactt aaatatctgt aaacccatta     3480

tatcgggttt ttgaggggat ttcaagtctt taagaagata ccaggcaatc aattaagaaa     3540

aacttagttg attgcctttt ttgttgtgat tcaactttga tcgtagcttc taactaatta     3600

attttcgtaa gaaaggagaa cagctgaatg aatatccctt ttgttgtaga aactgtgctt     3660

catgacggct tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat cactaccaag     3720

ccaggtaaaa gtaaaggggc tatttttgcg tatcgctcaa aaaaaagcat gattggcgga     3780

cgtggcgttg ttctgacttc cgaagaagcg attcacgaaa atcaagatac atttacgcat     3840

tggacaccaa acgtttatcg ttatggtacg tatgcagacg aaaaccgttc atacactaaa     3900

ggacattctg aaaacaattt aagacaaatc aataccttct ttattgattt tgatattcac     3960

acggaaaaag aaactatttc agcaagcgat attttaacaa cagctattga tttaggtttt     4020

atgcctacgt taattatcaa atctgataaa ggttatcaag catattttgt tttagaaacg     4080

ccagtctatg tgacttcaaa atcagaattt aaatctgtca aagcagccaa aataatctcg     4140

caaaatatcc gagaatattt tggaaagtct ttgccagttg atctaacgtg caatcatttt     4200

gggattgctc gtataccaag aacggacaat gtagaatttt ttgatcccaa ttaccgttat     4260

tctttcaaag aatggcaaga ttggtctttc aaacaaacag ataataaggg ctttactcgt     4320

tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac aagtagatga accctggttt     4380

aatctcttat tgcacgaaac gaaattttca ggagaaaagg gtttagtagg gcgcaatagc     4440

gttatgttta ccctctcttt agcctacttt agttcaggct attcaatcga aacgtgcgaa     4500

tataatatgt ttgagtttaa taatcgatta gatcaaccct tagaagaaaa agaagtaatc     4560

aaaattgtta gaagtgccta ttcagaaaac tatcaagggg ctaataggga atacattacc     4620

attctttgca aagcttgggt atcaagtgat ttaaccagta aagatttatt tgtccgtcaa     4680

gggtggttta aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg     4740

aaagaagatt taatggctta tattagcgaa aaaagcgatg tatacaagcc ttatttagcg     4800

acgaccaaaa aagagattag agaagtgcta ggcattcctg aacggacatt agataaattg     4860

ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt     4920

ggcattcaac ttgctagtgt taaatcattg ttgctatcga tcattaaatt aaaaaaagaa     4980

gaacgagaaa gctatataaa ggcgctgaca gcttcgttta atttagaacg tacatttatt     5040

caagaaactc taaacaaatt ggcagaacgc cccaaaacgg acccacaact cgatttgttt     5100

agctacgata caggctgaaa ataaaacccg cactatgcca ttacatttat atctatgata     5160

cgtgtttgtt tttctttgct ggctagctta attgcttata tttacctgca ataaaggatt     5220

tcttacttcc attatactcc cattttccaa aaacatacgg ggaacacggg aacttattgt     5280

acaggccacc tcatagttaa tggtttcgag ccttcctgca atctcatcca tggaaatata     5340

ttcatccccc tgccggccta ttaatgtgac ttttgtgccc ggcggatatt cctgatccag     5400

ctccaccata aattggtcca tgcaaattcg gccggcaatt ttcaggcgtt ttcccttcac     5460

aaggatgtcg gtccctttca attttcggag ccagccgtcc gcatagccta caggcaccgt     5520

cccgatccat gtgtcttttt ccgctgtgta ctcggctccg tagctgacgc tctcgccttt     5580

tctgatcagt ttgacatgtg acagtgtcga atgcagggta aatgccggac gcagctgaaa     5640

cggtatctcg tccgacatgt cagcagacgg gcgaaggcca tacatgccga tgccgaatct     5700

gactgcatta aaaaagcctt ttttcagccg gagtccagcg gcgctgttcg cgcagtggac     5760

cattagattc tttaacggca gcggagcaat cagctcttta aagcgctcaa actgcattaa     5820

gaaatagcct ctttcttttt catccgctgt cgcaaaatgg gtaaataccc ctttgcactt     5880

taaacgaggg ttgcggtcaa gaattgccat cacgttctga acttcttcct ctgtttttac     5940

accaagtctg ttcatccccg tatcgacctt cagatgaaaa tgaagagaac cttttttcgt     6000

gtggcgggct gcctcctgaa gccattcaac agaataacct gttaaggtca cgtcatactc     6060

agcagcgatt gccacatact ccgggggaac cgcgccaagc accaatatag gcgccttcaa     6120

tccctttttg cgcagtgaaa tcgcttcatc caaaatggcc acggccaagc atgaagcacc     6180

tgcgtcaaga gcagcctttg ctgtttctgc atcaccatgc ccgtaggcgt ttgctttcac     6240

aactgccatc aagtggacat gttcaccgat atgttttttc atattgctga cattttcctt     6300

tatcgcggac aagtcaattt ccgcccacgt atctctgtaa aaaggttttg tgctcatgga     6360

aaactcctct cttttttcag aaaatcccag tacgtaatta agtatttgag aattaatttt     6420

atattgatta atactaagtt tacccagttt tcacctaaaa aacaaatgat gagataatag     6480

ctccaaaggc taaagaggac tataccaact atttgttaat taa                       6523


<210>  35
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
cggaattcgg atccgcgcca aatcattggt tgattg                                 36


<210>  36
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
gcgagtcgac gtcggggtta atcgtaatgc aattggc                                37


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
gcgagtcgac ccatacgacg ttaattcttg caatg                                  35


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
gatactgcag ggatccttcc cttctcggta atcagtcac                              39


<210>  39
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
tgggatggcc aagaaattc                                                    19


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
ctaccatgtc ttccgttgct tg                                                22


<210>  41
<211>  364
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lovaxin_C_pGG55

<400>  41
ccaaacccta caaaaacaag tttcatacag cctagctaaa tttaatgttt tttcgattaa       60

cgggaagctt ggctctattt gcggtcaact tttaatcctg acctatgtgt atggtaaaga      120

aactcctgat ggcatcaaga ttacactgga taatttaaca atgcaggagt taggatattc      180

aagtggcatc gcacatagct cagctgttag cagaattatt tccaaattaa agcaagagaa      240

agttatcgtg tataaaaatt catgctttta tgtacaaaat cgtgattatc tcaaaagata      300

tgcccctaaa ttagatgaat ggttttattt agcatgtcct gctacttggg gaaaattaaa      360

ttaa                                                                   364


<210>  42
<211>  364
<212>  DNA
<213>  Listeria monocytogenes

<400>  42
ccaaacccta caaaaacaag tttcatacag cctagctaaa tttaatgatt tttcgattaa       60

cgggaagctt ggctctattt gcggtcaact tttaatcctg acctatgtgt atggtaaaga      120

aactcctgat ggcatcaaga ttacactgga taatttaaca atgcaggagt taggatattc      180

aagtggcatc gcacatagct cagctgttag cagaattatt tccaaattaa agcaagagaa      240

agttatcgtg tataaaaatt catgctttta tgtacaaaat cttgattatc tcaaaagata      300

tgcccctaaa ttagatgaat ggttttattt agcatgtcct gctacttggg gaaaattaaa      360

ttaa                                                                   364


