                         SEQUENCE LISTING

<110>  Yang, Xiang-Lei
       Mo, Zhongying
       Schimmel, Paul
 
<120>  INHIBITION OF NEDDYLATION USING GLYCYL-tRNA SYNTHETASE INHIBITORS

<130>  AARS.002WO

<150>  62/241386
<151>  2015-10-14

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  2220
<212>  DNA
<213>  Homo sapiens

<400>  1
atgccctctc cgcgtccagt gctgcttaga ggtgctcgcg ccgctctgct gctgctgctg       60

ccgccccggc tcttagcccg accctcgctc ctgctccgcc ggtccctcag cgcggcctcc      120

tgccccccga tctccttgcc cgccgccgcc tcccggagca gcatggacgg cgcgggggct      180

gaggaggtgc tggcacctct gaggctagca gtgcgccagc agggagatct tgtgcgaaaa      240

ctcaaagaag ataaagcacc ccaagtagac gtagacaaag cagtggctga gctcaaagcc      300

cgcaagaggg ttctggaagc aaaggagctg gcgttacagc ccaaagatga tattgtagac      360

cgagcaaaaa tggaagatac cctgaagagg aggtttttct atgatcaagc ttttgctatt      420

tatggaggtg ttagtggtct gtatgacttt gggccagttg gctgtgcttt gaagaacaat      480

attattcaga cctggaggca gcactttatc caagaggaac agatcctgga gatcgattgc      540

accatgctca cccctgagcc agttttaaag acctctggcc atgtagacaa atttgctgac      600

ttcatggtga aagacgtaaa aaatggagaa tgttttcgtg ctgaccatct attaaaagct      660

catttacaga aattgatgtc tgataagaag tgttctgtcg aaaagaaatc agaaatggaa      720

agtgttttgg cccagcttga taactatgga cagcaagaac ttgcggatct ttttgtgaac      780

tataatgtaa aatctcccat tactggaaat gatctatccc ctccagtgtc ttttaactta      840

atgttcaaga ctttcattgg gcctggagga aacatgcctg ggtacttgag accagaaact      900

gcacagggga ttttcttgaa tttcaaacga cttttggagt tcaaccaagg aaagttgcct      960

tttgctgctg cccagattgg aaattctttt agaaatgaga tctcccctcg atctggactg     1020

atcagagtca gagaattcac aatggcagaa attgagcact ttgtagatcc cagtgagaaa     1080

gaccacccca agttccagaa tgtggcagac cttcaccttt atttgtattc agcaaaagcc     1140

caggtcagcg gacagtccgc tcggaaaatg cgcctgggag atgctgttga acagggtgtg     1200

attaataaca cagtattagg ctatttcatt ggccgcatct acctctacct cacgaaggtt     1260

ggaatatctc cagataaact ccgcttccgg cagcacatgg agaatgagat ggcccattat     1320

gcctgtgact gttgggatgc agaatccaaa acatcctacg gttggattga gattgttgga     1380

tgtgctgatc gttcctgtta tgacctctcc tgtcatgcac gagccaccaa agtcccactt     1440

gtagctgaga aacctctgaa agaacccaaa acagtcaatg ttgttcagtt tgaacccagt     1500

aagggagcaa ttggtaaggc atataagaag gatgcaaaac tggtgatgga gtatcttgcc     1560

atttgtgatg agtgctacat tacagaaatg gagatgctgc tgaatgagaa aggggaattc     1620

acaattgaaa ctgaagggaa aacatttcag ttaacaaaag acatgatcaa tgtgaagaga     1680

ttccagaaaa cactatatgt ggaagaagtt gttccgaatg taattgaacc ttccttcggc     1740

ctgggtagga tcatgtatac ggtatttgaa catacattcc atgtacgaga aggagatgaa     1800

cagagaacat tcttcagttt ccctgctgta gttgctccat tcaaatgttc cgtcctccca     1860

ctgagccaaa accaggagtt catgccattt gtcaaggaat tatcggaagc cctgaccagg     1920

catggagtat ctcacaaagt agacgattcc tctgggtcaa tcggaaggcg ctatgccagg     1980

actgatgaga ttggcgtggc ttttggtgtc accattgact ttgacacagt gaacaagacc     2040

ccccacactg caactctgag ggaccgtgac tcaatgcggc agataagagc agagatctct     2100

gagctgccca gcatagtcca agacctagcc aatggcaaca tcacatgggc tgatgtggag     2160

gccaggtatc ctctgtttga agggcaagag actggtaaaa aagagacaat cgaggaatga     2220


<210>  2
<211>  685
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asp Gly Ala Gly Ala Glu Glu Val Leu Ala Pro Leu Arg Leu Ala 
1               5                   10                  15      


Val Arg Gln Gln Gly Asp Leu Val Arg Lys Leu Lys Glu Asp Lys Ala 
            20                  25                  30          


Pro Gln Val Asp Val Asp Lys Ala Val Ala Glu Leu Lys Ala Arg Lys 
        35                  40                  45              


Arg Val Leu Glu Ala Lys Glu Leu Ala Leu Gln Pro Lys Asp Asp Ile 
    50                  55                  60                  


Val Asp Arg Ala Lys Met Glu Asp Thr Leu Lys Arg Arg Phe Phe Tyr 
65                  70                  75                  80  


Asp Gln Ala Phe Ala Ile Tyr Gly Gly Val Ser Gly Leu Tyr Asp Phe 
                85                  90                  95      


Gly Pro Val Gly Cys Ala Leu Lys Asn Asn Ile Ile Gln Thr Trp Arg 
            100                 105                 110         


Gln His Phe Ile Gln Glu Glu Gln Ile Leu Glu Ile Asp Cys Thr Met 
        115                 120                 125             


Leu Thr Pro Glu Pro Val Leu Lys Thr Ser Gly His Val Asp Lys Phe 
    130                 135                 140                 


Ala Asp Phe Met Val Lys Asp Val Lys Asn Gly Glu Cys Phe Arg Ala 
145                 150                 155                 160 


Asp His Leu Leu Lys Ala His Leu Gln Lys Leu Met Ser Asp Lys Lys 
                165                 170                 175     


Cys Ser Val Glu Lys Lys Ser Glu Met Glu Ser Val Leu Ala Gln Leu 
            180                 185                 190         


Asp Asn Tyr Gly Gln Gln Glu Leu Ala Asp Leu Phe Val Asn Tyr Asn 
        195                 200                 205             


Val Lys Ser Pro Ile Thr Gly Asn Asp Leu Ser Pro Pro Val Ser Phe 
    210                 215                 220                 


Asn Leu Met Phe Lys Thr Phe Ile Gly Pro Gly Gly Asn Met Pro Gly 
225                 230                 235                 240 


Tyr Leu Arg Pro Glu Thr Ala Gln Gly Ile Phe Leu Asn Phe Lys Arg 
                245                 250                 255     


Leu Leu Glu Phe Asn Gln Gly Lys Leu Pro Phe Ala Ala Ala Gln Ile 
            260                 265                 270         


Gly Asn Ser Phe Arg Asn Glu Ile Ser Pro Arg Ser Gly Leu Ile Arg 
        275                 280                 285             


Val Arg Glu Phe Thr Met Ala Glu Ile Glu His Phe Val Asp Pro Ser 
    290                 295                 300                 


Glu Lys Asp His Pro Lys Phe Gln Asn Val Ala Asp Leu His Leu Tyr 
305                 310                 315                 320 


Leu Tyr Ser Ala Lys Ala Gln Val Ser Gly Gln Ser Ala Arg Lys Met 
                325                 330                 335     


Arg Leu Gly Asp Ala Val Glu Gln Gly Val Ile Asn Asn Thr Val Leu 
            340                 345                 350         


Gly Tyr Phe Ile Gly Arg Ile Tyr Leu Tyr Leu Thr Lys Val Gly Ile 
        355                 360                 365             


Ser Pro Asp Lys Leu Arg Phe Arg Gln His Met Glu Asn Glu Met Ala 
    370                 375                 380                 


His Tyr Ala Cys Asp Cys Trp Asp Ala Glu Ser Lys Thr Ser Tyr Gly 
385                 390                 395                 400 


Trp Ile Glu Ile Val Gly Cys Ala Asp Arg Ser Cys Tyr Asp Leu Ser 
                405                 410                 415     


Cys His Ala Arg Ala Thr Lys Val Pro Leu Val Ala Glu Lys Pro Leu 
            420                 425                 430         


Lys Glu Pro Lys Thr Val Asn Val Val Gln Phe Glu Pro Ser Lys Gly 
        435                 440                 445             


Ala Ile Gly Lys Ala Tyr Lys Lys Asp Ala Lys Leu Val Met Glu Tyr 
    450                 455                 460                 


Leu Ala Ile Cys Asp Glu Cys Tyr Ile Thr Glu Met Glu Met Leu Leu 
465                 470                 475                 480 


Asn Glu Lys Gly Glu Phe Thr Ile Glu Thr Glu Gly Lys Thr Phe Gln 
                485                 490                 495     


Leu Thr Lys Asp Met Ile Asn Val Lys Arg Phe Gln Lys Thr Leu Tyr 
            500                 505                 510         


Val Glu Glu Val Val Pro Asn Val Ile Glu Pro Ser Phe Gly Leu Gly 
        515                 520                 525             


Arg Ile Met Tyr Thr Val Phe Glu His Thr Phe His Val Arg Glu Gly 
    530                 535                 540                 


Asp Glu Gln Arg Thr Phe Phe Ser Phe Pro Ala Val Val Ala Pro Phe 
545                 550                 555                 560 


Lys Cys Ser Val Leu Pro Leu Ser Gln Asn Gln Glu Phe Met Pro Phe 
                565                 570                 575     


Val Lys Glu Leu Ser Glu Ala Leu Thr Arg His Gly Val Ser His Lys 
            580                 585                 590         


Val Asp Asp Ser Ser Gly Ser Ile Gly Arg Arg Tyr Ala Arg Thr Asp 
        595                 600                 605             


Glu Ile Gly Val Ala Phe Gly Val Thr Ile Asp Phe Asp Thr Val Asn 
    610                 615                 620                 


Lys Thr Pro His Thr Ala Thr Leu Arg Asp Arg Asp Ser Met Arg Gln 
625                 630                 635                 640 


Ile Arg Ala Glu Ile Ser Glu Leu Pro Ser Ile Val Gln Asp Leu Ala 
                645                 650                 655     


Asn Gly Asn Ile Thr Trp Ala Asp Val Glu Ala Arg Tyr Pro Leu Phe 
            660                 665                 670         


Glu Gly Gln Glu Thr Gly Lys Lys Glu Thr Ile Glu Glu 
        675                 680                 685 


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  3
gcatggagta tctcacaaag t                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
gcccaaagat gatattgtag a                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  5
gctgcccaga ttggaaattc t                                                 21


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  6
ggcgttacag cccaaagat                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
gcccaaagat gatattgta                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  8
cctggaggca gcactttat                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  9
gctgcccaga ttggaaatt                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  10
ggagcaattg gtaaggcat                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
gcaattggta aggcatata                                                    19


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
ccgaatgtaa ttgaacctt                                                    19


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
gcctgggtag gatcatgta                                                    19


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
ggagatgaac agagaacat                                                    19


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
gcatggagta tctcacaaa                                                    19


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
ccagaatgtg gcagacctt                                                    19


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  17
cctgggtagg atcatgtat                                                    19


<210>  18
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  18
ggcccagctt gataactat                                                    19


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  19
gggtacttga gaccagaaa                                                    19


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  20
ggcagaaatt gagcacttt                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  21
ggaagaagtt gttccgaat                                                    19


<210>  22
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  22
ggatcatgta tacggtatt                                                    19


<210>  23
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  23
ggcatggagt atctcacaa                                                    19


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  24
cctatgcttt gaaggttct                                                    19


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  25
gctttgaagg ttctcgtgt                                                    19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
tcagagctgt gtccctgaa                                                    19


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
gcaaatctgt tcgctcgca                                                    19


<210>  28
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
gcggcgattt catcatgct                                                    19


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
gcgatttcat catgctccg                                                    19


<210>  30
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  30
cccaaagatg atattgtag                                                    19


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  31
gctgtgcttt gaagaacaa                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  32
gcactttatc caagaggaa                                                    19


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
tcccattact ggaaatgat                                                    19


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
gctatttcat tggccgcat                                                    19


<210>  35
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
gcatctacct ctacctcac                                                    19

<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
ggcataggga cccatcattg a                                                 21


