                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Glucoamylase variants having increased specific activity and/or 
       increased thermal stability

<130>  13323-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1722
<212>  DNA
<213>  Gloeophyllum sepiarium

<400>  1
atgtaccgct tccttgtctg tgcgctgggg cttgcggcat cagttctcgc ccagtcggtc       60

gacagctatg ttagcagcga aggtcccata gccaaggcgg gcgtccttgc taacattggg      120

ccgaacggct ccaaggcctc tggcgcatcc gctggtgttg tggtcgcgag ccctagcacg      180

tcggaccccg actattggta cacttggacg cgtgactcgt ccctcgtatt caagtcactt      240

attgaccagt acaccaccgg catcgacagc acgagctctc tgaggactct catcgacgat      300

ttcgtaactg ccgaggctaa tctccagcaa gtctctaacc ctagtggtac cctcaccacc      360

ggtggcttgg gagagcccaa gttcaacgtc gacgaaactg catttactgg tgcatggggt      420

cgaccccaac gcgacggacc tgccctccgc tcgactgcat tgatcacgta cggtaactgg      480

ctgttgtcca acggaaatac gagctatgtt acgagcaatc tgtggccgat catccagaac      540

gaccttggtt atgtcgtgtc atactggaac cagtctacct acgacctctg ggaggaagta      600

gactcgtcat cgttcttcac tactgcagta cagcaccgtg ctctccgtga aggtgcggcc      660

ttcgctaccg ccatcggtca gacttcgcag gtcagcagct atacgactca ggcggacaat      720

cttctgtgct tcttgcagtc ttactggaac ccgagcggtg gttacatcac tgctaacact      780

ggcggcggcc gttccggcaa ggatgccaac acacttctgg catccattca cacgtacgac      840

cccagcgcgg gctgcgacgc tgcgactttc cagccctgct ctgacaaggc actgtcgaac      900

ctgaaggtct acgtcgactc tttccgctcg gtctactcca tcaacagtgg tgtcgcctct      960

aacgctgccg tcgccacggg tcgttatccc gaggatagct accagggtgg aaacccttgg     1020

tacctcacca catttgcggt cgccgagcaa ctctatgatg ctctcaatgt ctgggagtcg     1080

cagggttccc tcgaggtcac ctccacctcc cttgccttct tccagcagtt ctcatccggc     1140

gtcactgctg gcacctactc ttctagctcc agcacataca gcaccctcac gtctgccatc     1200

aagaactttg ccgatggatt tgtcgctatc aatgctaagt acacgccatc caacggtggc     1260

ctggcggaac aatacagcaa gagcgacggt tctcccctta gcgcggtgga cttgacgtgg     1320

agctacgctt cggctttgac ggcgtttgaa gcaaggaaca atactcagtt cgccggctgg     1380

ggcgctgcag gcctgactgt gccttcctct tgctccggca actctggtgg gccgaccgtt     1440

gctgtcacat tcaacgtgaa cgccgagact gtgtggggag agaacatcta tcttactggt     1500

tccgtcgatg ctctggagaa ctggtcggcc gacaatgccc tcctgctctc atcggctaat     1560

tacccgacct ggagtatcac cgtcaacttg ccggcgagca ctgctattga gtacaagtac     1620

atccgcaaaa ataatggggc cgttacctgg gagtcagacc ccaacaatag catcactact     1680

ccggccagcg gctcgacgac cgagaatgac acttggcgtt ga                        1722


<210>  2
<211>  573
<212>  PRT
<213>  Gloeophyllum sepiarium

<400>  2

Met Tyr Arg Phe Leu Val Cys Ala Leu Gly Leu Ala Ala Ser Val Leu 
1               5                   10                  15      


Ala Gln Ser Val Asp Ser Tyr Val Ser Ser Glu Gly Pro Ile Ala Lys 
            20                  25                  30          


Ala Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly 
        35                  40                  45              


Ala Ser Ala Gly Val Val Val Ala Ser Pro Ser Thr Ser Asp Pro Asp 
    50                  55                  60                  


Tyr Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu 
65                  70                  75                  80  


Ile Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Thr 
                85                  90                  95      


Leu Ile Asp Asp Phe Val Thr Ala Glu Ala Asn Leu Gln Gln Val Ser 
            100                 105                 110         


Asn Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe 
        115                 120                 125             


Asn Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg 
    130                 135                 140                 


Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Gly Asn Trp 
145                 150                 155                 160 


Leu Leu Ser Asn Gly Asn Thr Ser Tyr Val Thr Ser Asn Leu Trp Pro 
                165                 170                 175     


Ile Ile Gln Asn Asp Leu Gly Tyr Val Val Ser Tyr Trp Asn Gln Ser 
            180                 185                 190         


Thr Tyr Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr 
        195                 200                 205             


Ala Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Ala 
    210                 215                 220                 


Ile Gly Gln Thr Ser Gln Val Ser Ser Tyr Thr Thr Gln Ala Asp Asn 
225                 230                 235                 240 


Leu Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Ile 
                245                 250                 255     


Thr Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu 
            260                 265                 270         


Leu Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Ala 
        275                 280                 285             


Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr 
    290                 295                 300                 


Val Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Val Ala Ser 
305                 310                 315                 320 


Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly 
                325                 330                 335     


Gly Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr 
            340                 345                 350         


Asp Ala Leu Asn Val Trp Glu Ser Gln Gly Ser Leu Glu Val Thr Ser 
        355                 360                 365             


Thr Ser Leu Ala Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Ala Gly 
    370                 375                 380                 


Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile 
385                 390                 395                 400 


Lys Asn Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Lys Tyr Thr Pro 
                405                 410                 415     


Ser Asn Gly Gly Leu Ala Glu Gln Tyr Ser Lys Ser Asp Gly Ser Pro 
            420                 425                 430         


Leu Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala 
        435                 440                 445             


Phe Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Ala Gly 
    450                 455                 460                 


Leu Thr Val Pro Ser Ser Cys Ser Gly Asn Ser Gly Gly Pro Thr Val 
465                 470                 475                 480 


Ala Val Thr Phe Asn Val Asn Ala Glu Thr Val Trp Gly Glu Asn Ile 
                485                 490                 495     


Tyr Leu Thr Gly Ser Val Asp Ala Leu Glu Asn Trp Ser Ala Asp Asn 
            500                 505                 510         


Ala Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val 
        515                 520                 525             


Asn Leu Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys Asn 
    530                 535                 540                 


Asn Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr 
545                 550                 555                 560 


Pro Ala Ser Gly Ser Thr Thr Glu Asn Asp Thr Trp Arg 
                565                 570             


<210>  3
<211>  556
<212>  PRT
<213>  Gloeophyllum sepiarium

<400>  3

Gln Ser Val Asp Ser Tyr Val Ser Ser Glu Gly Pro Ile Ala Lys Ala 
1               5                   10                  15      


Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly Ala 
            20                  25                  30          


Ser Ala Gly Val Val Val Ala Ser Pro Ser Thr Ser Asp Pro Asp Tyr 
        35                  40                  45              


Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu Ile 
    50                  55                  60                  


Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Thr Leu 
65                  70                  75                  80  


Ile Asp Asp Phe Val Thr Ala Glu Ala Asn Leu Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Gly Asn Trp Leu 
    130                 135                 140                 


Leu Ser Asn Gly Asn Thr Ser Tyr Val Thr Ser Asn Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Gln Asn Asp Leu Gly Tyr Val Val Ser Tyr Trp Asn Gln Ser Thr 
                165                 170                 175     


Tyr Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Ala Ile 
        195                 200                 205             


Gly Gln Thr Ser Gln Val Ser Ser Tyr Thr Thr Gln Ala Asp Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Ala Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Val Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Asn Val Trp Glu Ser Gln Gly Ser Leu Glu Val Thr Ser Thr 
            340                 345                 350         


Ser Leu Ala Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Ala Gly Thr 
        355                 360                 365             


Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Asn Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Lys Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Tyr Ser Lys Ser Asp Gly Ser Pro Leu 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala Phe 
            420                 425                 430         


Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Ala Gly Leu 
        435                 440                 445             


Thr Val Pro Ser Ser Cys Ser Gly Asn Ser Gly Gly Pro Thr Val Ala 
    450                 455                 460                 


Val Thr Phe Asn Val Asn Ala Glu Thr Val Trp Gly Glu Asn Ile Tyr 
465                 470                 475                 480 


Leu Thr Gly Ser Val Asp Ala Leu Glu Asn Trp Ser Ala Asp Asn Ala 
                485                 490                 495     


Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val Asn 
            500                 505                 510         


Leu Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys Asn Asn 
        515                 520                 525             


Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr Pro 
    530                 535                 540                 


Ala Ser Gly Ser Thr Thr Glu Asn Asp Thr Trp Arg 
545                 550                 555     


