                         SEQUENCE LISTING

<110>  Chaput, John
       Larsen, Andrew
       Dunn, Matthew
 
<120>  HIGHLY SENSITIVE OPTICAL SENSOR FOR POLYMERASE SCREENING

<130>  11157.013

<150>  62/239,178
<151>  2015-10-08

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  228
<212>  DNA template
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (106)..(108)
<223>  n represents a degenerate position containing an equal 
       distribution of A, T, G, and C nucleobases

<400>  1
gaacgtgaac tggcgcgccg tcgtggcggt tatgcgggcg gttatgtgaa agaaccggaa       60

cgtggcctgt gggataacat tgtgtatctg gattttcgta gcctgnnncc gagcattatt      120

atcacccaca atgtgagccc ggataccctg aaccgtgaag gctgcaaaga atatgatgtg      180

gcgccggaag tgggccataa attctgcaaa gatttcccgg gctttatt                   228


<210>  2
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (126)..(128)
<223>  N represents a degenerate position containing an equal 
       distribution of A, T, G, and C nucleobases

<400>  2
aagatttccc gggctttatt ccgagcctgc tgggcgatct gctcgaggaa cgccagaaaa       60

tcaaacgcaa aatgaaagcg accgttgatc cgctggaaaa aaaactgctg gattatcgtc      120

agcgcnnnat taaaattctg gccaacagct tctatggcta ttatggttat gcgaaagcgc      180

gttggtattg caaagaatgc gcggaaagcg tgaccgcgtg gggccgtgaa tatatcgaaa      240

tggtgatccg cgagctcgaa gaaaaattcg gcttcaaagt gctgtatgcg gataccgatg      300

gcctgcatgc gaccattccg ggtgcggatg cggaaaccgt gaaaaaaaaa gcgaaagaat      360

tcctgaaata catcaatccg aaactgccgg gcctgctgga actggaatat gaaggctttt      420

atgtgcgtgg ctttttcgtg accaaaaaaa aatacgcggt gatcgatgaa gaaggcaaaa      480

ttaccacccg tggcctggaa                                                  500


<210>  3
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (213)..(215)
<223>  N represents a degenerate position containing an equal 
       distribution of A, T, G, and C nucleobases

<400>  3
aatacgcggt gatcgatgaa gaaggcaaaa ttaccacccg tggcctggaa attgtgcgtc       60

gtgattggag cgaaattgcg aaagaaaccc aggcgcgtgt gctggaagcg attctgaaac      120

atggcgatgt ggaagaagcg gtgcgtattg ttaaagaagt gaccgaaaaa ctgagcaaat      180

atgaggtacc gccggaaaaa ctggtgattc atnnncaaat tacccgtgat ctgcgtgatt      240

ataaagcgac cggtccgcat gtggcggtgg caaaacgtct ggcagcgcgt ggcgtgaaaa      300

ttcgtccggg caccgtgatt agctatattg tgctgaaagg cagcggccgc attggcgatc      360

gtgcgattcc ggcggatgaa tttgatccga ccaaacatcg ttatgatgcg gaatattata      420

tcgaaaacca ggtgctgccg gcggtggaac gtattctgaa agcgtttggc tatcgtaaag      480

aagatctgcg ctatc                                                       495


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
aactggcgcg ccgtcgtggc ggttatgcgg                                        30


<210>  5
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
cgttcctcga gcagatcgcc cagcaggctc ggaataaag                              39


<210>  6
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
atctgctcga ggaacgccag aaaatcaaac gc                                     32


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
ttccaggcca cgggtggtaa ttttgc                                            26


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
aatacgcggt gatcgatgaa g                                                 21


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
gatagcgcag atcttcttta cgatagcc                                          28


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' IRDye 800

<400>  10
gacactcgta tgcagtagcc                                                   20


<210>  11
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Cy3

<400>  11
acaaccatac tctcctcatc actattcaac ttacaatcga tacaacctta taatccacat       60

ggctactgca tacgagtgtc                                                   80


<210>  12
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' 6-FAM

<400>  12
acaaccatac tctcctcatc actattcaac ttacaatcga tacaacctta taatccacat       60

ggctactgca tacgagtgtc                                                   80


<210>  13
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template

<400>  13
acaaccatac tctcctcatc actattcaac ttacaatcga tacaacctta taatccacat       60

ggctactgca tacgagtgtc                                                   80


<210>  14
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  14
agagtatggt tgt                                                          13


<210>  15
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  3' Iowa Black FQ

<400>  15
aggagagtat ggttgt                                                       16


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  3' Iowa Black FQ

<400>  16
gatgaggaga gtatggttgt                                                   20


<210>  17
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  3' Black Hole Quencher-1

<400>  17
agagtatggt tgt                                                          13


<210>  18
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  3' Black Hole Quencher-1

<400>  18
aggagagtat ggttgt                                                       16


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  3' Black Hole Quencher.-1

<400>  19
gatgaggaga gtatggttgt                                                   20


<210>  20
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template


<220>
<221>  misc_feature
<222>  (90)..(90)
<223>  3' Inverted dT

<400>  20
tgtctacacg caagcttaca ttaagactcg ccatgttacg atctgccaag tacagccttg       60

aatcgtcact ggctactgca tacgagtgtc                                        90


<210>  21
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc                             40


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
tgtctacacg caagcttaca                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
cttttaagaa ccggacgaac                                                   20


<210>  24
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  24
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  25
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  25
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  26
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  26
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  27
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  27
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  28
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  28
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  29
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  29
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  30
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  30
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  31
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  31
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  32
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  32
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  33
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  33
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  34
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  34
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttca acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  35
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  35
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  36
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  36
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  37
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  37
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaatctc tgtctacacg caagcttaca                 110


<210>  38
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  38
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  39
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  39
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tacaagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  40
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  40
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  41
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  41
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  42
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  42
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  43
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  43
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  44
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  44
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  45
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  45
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacccc tgtctacacg caagcttaca                 110


<210>  46
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  46
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  47
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  47
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa cattaacctc tgtctacacg caagcttaca                 110


<210>  48
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fidelity assay reads

<400>  48
cttttaagaa ccggacgaac gacactcgtt tgcagtagcc ccattcttta acagctcatc       60

actagacatt tataagtcaa caataacctc tgtctacacg caagcttaca                 110


