                               SEQUENCE LISTING

<110> GENZYME CORPORATION
 
<120> IMPROVED FLARE (FLOW CYTOMETRY ATTENUATED REPORTER EXPRESSION) 
      TECHNOLOGY FOR RAPID BULK SORTING

<130> 584603: SA9-179PC

<140> PCT/US2016/055915
<141> 2016-10-07

<150> 62/239,515
<151> 2015-10-09

<160> 8     

<170> PatentIn version 3.5

<210> 1
<211> 61
<212> PRT
<213> Homo sapiens

<400> 1
Leu Glu Arg Phe Leu Phe Leu Leu Leu Thr Ile Ser Leu Leu Val Leu 
1               5                   10                  15      


Val Gln Ile Gln Thr Gly Leu Ser Gly Gln Asn Asp Thr Ser Gln Thr 
            20                  25                  30          


Ser Ser Pro Ser Ala Ser Ser Asn Ile Ser Gly Gly Ile Phe Leu Phe 
        35                  40                  45              


Phe Val Ala Asn Ala Ile Ile His Leu Phe Cys Phe Ser 
    50                  55                  60      


<210> 2
<211> 128
<212> PRT
<213> Homo sapiens

<400> 2
Leu Gly Ile Gln Gly Gly Ser Val Leu Phe Gly Leu Leu Leu Val Leu 
1               5                   10                  15      


Ala Val Phe Cys His Ser Gly His Ser Leu Gln Cys Tyr Asn Cys Pro 
            20                  25                  30          


Asn Pro Thr Ala Asp Cys Lys Thr Ala Val Asn Cys Ser Ser Asp Phe 
        35                  40                  45              


Asp Ala Cys Leu Ile Thr Lys Ala Gly Leu Gln Val Tyr Asn Asn Cys 
    50                  55                  60                  


Trp Lys Phe Glu His Cys Asn Phe Asn Asp Val Thr Thr Arg Leu Arg 
65                  70                  75                  80  


Glu Asn Glu Leu Thr Tyr Tyr Cys Cys Lys Lys Asp Leu Cys Asn Phe 
                85                  90                  95      


Asn Glu Gln Leu Glu Asn Gly Gly Thr Ser Leu Ser Glu Lys Thr Val 
            100                 105                 110         


Leu Leu Leu Val Thr Pro Phe Leu Ala Ala Ala Trp Ser Leu His Pro 
        115                 120                 125             


<210> 3
<211> 74
<212> PRT
<213> Mus sp.

<400> 3
Leu Lys Ser Phe Leu Leu Phe Leu Thr Ile Ile Leu Leu Val Val Ile 
1               5                   10                  15      


Gln Ile Gln Thr Gly Ser Leu Gly Gln Ala Thr Thr Ala Ala Ser Gly 
            20                  25                  30          


Thr Asn Lys Asn Ser Thr Ser Thr Lys Lys Thr Pro Leu Lys Ser Gly 
        35                  40                  45              


Ala Ser Ser Ile Ile Asp Ala Gly Ala Cys Ser Phe Leu Phe Phe Ala 
    50                  55                  60                  


Asn Thr Leu Ile Cys Leu Phe Tyr Leu Ser 
65                  70                  


<210> 4
<211> 186
<212> DNA
<213> Homo sapiens

<400> 4
ttggagcgct tcctcttcct cctactcacc atcagcctcc tcgttttggt acaaatacaa       60

accggactct ccggacaaaa cgacaccagc caaaccagca gcccctcagc atccagcaac      120

ataagcggag gcattttcct tttcttcgtc gccaacgcca taatccacct cttctgcttc      180

agttga                                                                 186


<210> 5
<211> 387
<212> DNA
<213> Homo sapiens

<400> 5
ttgggaatcc aaggagggtc tgtcctgttc gggctgctgc tcgtcctcgc tgtcttctgc       60

cattccggtc atagcctgca gtgctacaac tgtcctaacc caactgctga ctgcaaaaca      120

gccgtcaatt gttcatctga ttttgacgcg tgtctcatta ccaaagctgg gttacaagtg      180

tataacaact gttggaagtt tgagcattgc aatttcaacg acgtcacaac ccgcttgagg      240

gaaaacgagc taacgtacta ctgctgcaag aaggacctgt gtaactttaa cgaacagctt      300

gaaaacggag ggacatcctt atcagagaaa acagttcttc tgctggtgac tccatttctg      360

gcagctgctt ggagccttca tccctaa                                          387


<210> 6
<211> 233
<212> DNA
<213> Mus sp.

<400> 6
ttgaagagct tcctcctctt cctcactatc attcttctcg tagtcattca gatacaaaca       60

ggatccttag gacaagccac tacggccgct tcaggtacta acaaaaacag cacctccacc      120

aaaaaaaccc ccttaaagag cggggcctca tccatcatcg acgcgggcgc ttgcagtttc      180

ctcttcttcg ccaataccct tatttgcctc ttctacctca gctaactgag taa             233


<210> 7
<211> 8123
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polynucleotide"

<400> 7
ggatccgctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg       60

cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg      120

ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc      180

gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca      240

tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt      300

ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgggggggg      360

ggacagctca gggctgcgat ttcgcgccaa acttgacggc aatcctagcg tgaaggctgg      420

taggatttta tccccgctgc catcatggtt cgaccattga actgcatcgt cgccgtgtcc      480

caaaatatgg ggattggcaa gaacggagac ctaccctggc ctccgctcag gaacgagttc      540

aagtacttcc aaagaatgac cacaacctct tcagtggaag gtaaacagaa tctggtgatt      600

atgggtagga aaacctggtt ctccattcct gagaagaatc gacctttaaa ggacagaatt      660

aatatagttc tcagtagaga actcaaagaa ccaccacgag gagctcattt tcttgccaaa      720

agtttggatg atgccttaag acttattgaa caaccggaat tggcaagtaa agtagacatg      780

gtttggatag tcggaggcag ttctgtttac caggaagcca tgaatcaacc aggccacctc      840

agactctttg tgacaaggat catgcaggaa tttgaaagtg acacgttttt cccagaaatt      900

gatttgggga aatataaact tctcccagaa tacccaggcg tcctctctga ggtccaggag      960

gaaaaaggca tcaagtataa gtttgaagtc tacgagaaga aagactaaca ggaagatgct     1020

ttcaagttct ctgctcccct cctaaagcta tgcattttta taagaccatg ggacttttgc     1080

tggctttaga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta     1140

cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa     1200

ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag     1260

cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt     1320

gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta     1380

gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt     1440

aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac     1500

aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat     1560

aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct     1620

caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg     1680

tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc     1740

tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt     1800

tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt     1860

cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt     1920

atcttatcat gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac     1980

aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca     2040

cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccgtg gccgggggac     2100

tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca     2160

acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc     2220

ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg     2280

ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct     2340

gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg     2400

cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac     2460

gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct     2520

tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg     2580

gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc     2640

agggacagct tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     2700

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     2760

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     2820

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     2880

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     2940

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     3000

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     3060

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     3120

ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta     3180

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     3240

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     3300

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     3360

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     3420

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     3480

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     3540

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     3600

gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     3660

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     3720

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag     3780

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     3840

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     3900

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     3960

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     4020

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac     4080

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     4140

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     4200

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     4260

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     4320

tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat     4380

acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa     4440

aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc     4500

gtatcacgag gccctttcgt cttcaagaat tggggaccaa gacagaacca taagccagtg     4560

ggatagatca gaaatgttcc agaggtggga tggggccaga gtgcctgccc cttgaaccgt     4620

cccagggacc agaggtgaca aagtggcaac acaggtcctg cctgggaatc tggtctgctc     4680

ctacttagta aagctgcctg gtgtcacaca agaggccccc acttattcct gcacccctgg     4740

tggtaggtgg cgtcttctcc cctgcagcca ccaggctccc ctgagaacac tgccggcagt     4800

cctcattgac aggcagtatt cgctctgccc cacccccacc tgtgaattgc agggctggca     4860

ggtcctcagg cagctggcaa accgcctgaa caactgagag atacagggcc agggccaggg     4920

cagtcccgtc ccccggaggc agggagggga cgtgctggga aagttctctc tctcaggccc     4980

aggttggtga ctgcagaagg cttctgtcaa atctcttttg tgggaaccac agagtagccc     5040

tgaacgtggg ggtgtgcttc cagtatactc tggggtcacc ctttccatac tggaggcctc     5100

tgcaacttca aaatgctctg ctaccaacct agcacaagga agttggtcca gcctccccac     5160

gcagggccac tgctgcagtc catatatgga ctaagccttc cttggtttca acacctacac     5220

tcactgagcc cctactatgt gtatgcagag ccgagacagg ccctgagcat ctcatctgaa     5280

gcacccttct tgcctaaatt cagttttctg tcactttctc ccaggaggtg tgtgtccctc     5340

taagctaagc caggggtccc tcacccctgc cccactccca tccctagtgt aggtatcagc     5400

tgaagagctt cctgagcaga acactcttgg gtgctgacat tttgataaat aggcccatgt     5460

ttaggagagc aggggtccgg gggcgggaga tcttctctgg tggattgagg gctccaagaa     5520

ctactctttg agcacgctgc ccctcccaga gtccccacag cctccagatg gactagaaca     5580

cagttcggct gtggctgcac ataactaaca gaggatagat ggtgggtccc agcccaacag     5640

tgcctggcaa tcacccagag ccaccagcta acggccttgg cttagttttt tgcctgggtg     5700

tgatcaggca gccctccaaa actgcccgga ctccatgaca agttttgctt gttctataga     5760

gcacagttcc tttctaggtc tggggcaagg gacatcggga gacatcttcc tgcaacagct     5820

ccagtcactg gaccaccagg ctcgccctgt ctttggtgtg tggccctgag tctcctaagt     5880

ggcccaaacc tgtgaagacc cctccaacca cagttttgct tctaaattgt accccaacac     5940

acctagcaaa ttgaaacccc accagaagtc ccccagatct ggctttccgg ctattgctgg     6000

caagggggag tgactcccgg cccattcaat ccaggccccg cgtgttcctc aaacaagaag     6060

ccacgtaaac ataaaccgag cctccatgct gacccttgcc catcgaggta ctcaatgttc     6120

acgtgatatc cacacccaga gggtcctggg gtgggtgcat gagccccaga atgcaggctt     6180

gataaccgag accctgaatc gggcagtgtc cacaagggcg gaggccagtc atgcatgttc     6240

gggcctatgg ggccagcacc caacgccaaa actctccatc ctcttcctca atctcgcttt     6300

ctctctctct ctcttttttt ttttttattt tttttttttg caaaaggagg ggagaggggg     6360

taaaaaaatg ctgcactgtg cggctaggcc ggtgagtgag cggcgcggag ccaatcagcg     6420

ctcgccgttc cgaaagttgc cttttatggc tcgagtggcc gctgtggcgt cctataaaac     6480

ccggcggcgc aacgcgcagc cactgtcgag tccgcgtcca cccgcgagca caggcctttc     6540

gcagctcttt cttcgccgct ccacacccgc caccaggtaa gcagggacaa caggcccagc     6600

cggccacagc cctcccgtgg gcagtgaccg cgctgcaggg tcgcggggga cactcggcgc     6660

ggacaccggg gaaggctgga gggtggtgcc gggccgcgga gcggacactt tcagatccaa     6720

ctttcagtcc agggtgtaga ccctttacag ccgcattgcc acggtgtaga caccggtgga     6780

cccgctctgg ctcagagcac gcggcttggg ggaacccatt agggtcgcag tgtgggcgct     6840

atgagagccg atgcagcttt cgggtgttga accgtatctg cccaccttgg ggggaggaca     6900

caaggtcggg agccaaacgc cacgatcatg ccttggtggc ccatgggtct ttgtctaaac     6960

cggtttgccc atttggcttg ccgggcgggc gggcgcggcg ggcccggctc ggccgggtgg     7020

gggctgggtt gccactgcgc ttgcgcgctc tatggctggg tattggggcg cgtgcacgct     7080

ggggagggag cccttcctct tccccctctc ccaagttaaa cttgcgcgtg cgtattgaga     7140

cttggagcgc ggccaccggg gttgggcgag ggcggggccg ttgtccggaa ggggcggggt     7200

cgcagcggct tcggggcgcc tgctcgcgct tcctgctggg tgtggtcgcc tcccgcgcgc     7260

gcactagccg cccgccggcg gggcgaaggc ggggcttgcg cccgtttggg gagggggcgg     7320

aggcctggct tcctgccgtg gggccgcctc cggaccagcg tttgcctctt atggtaataa     7380

cgcggccggc ctgggcttcc tttgtcccct gagtttgggc gcgcgccccc tggcggcccg     7440

aggccgcggc ttgccggaag tgggcagggc ggcagcggct gcgcctagtg gcccgctagt     7500

gaccgcgacc ctcttttgtg ccctgatata gttcgccgga tcctaccgcg gtagcggccg     7560

cgccaccttg gagcgcttcc tcttcctcct actcaccatc agcctcctcg ttttggtaca     7620

aatacaaacc ggactctccg gacaaaacga caccagccaa accagcagcc cctcagcatc     7680

cagcaacata agcggaggca ttttcctttt cttcgtcgcc aacgccataa tccacctctt     7740

ctgcttcagt tgaaggccgg ccaatacgta ggcgcgccat tgagtgagtg atttggcgcg     7800

ccaagatatc acacccggga ttaattaaag gtacctacgc gtagaattcc acgtagtggt     7860

ttaaactcta gatactcgag ggatctggat cataatcagc cataccacat ttgtagaggt     7920

tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc     7980

aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat     8040

cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact     8100

catcaatgta tcttatcatg tct                                             8123


<210> 8
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 8
gccrccatgg                                                              10


