SEQUENCE LISTING

<110>  Merial, Inc.
       Institut Pasteur
       Vetagro-Sup
       Bomchil , Natalia
       Cupillard , Lionel
       Fontan, CC)lia
       Guigal, Pierre-Michel
       Bouvet , JC)rC4me
       Picardeau, Mathieu
       Kodjo, Angeli
 
<120>  Live Attenuated Heterologous Vaccine for Leptospira

<130>  MER 15-269.PCT

<150>  62/239,136
<151>  2015-10-08

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  PflgA primer

<400>  1
taatacccga gcttcaagga ag                                                22


<210>  2
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  FliMR2 primer

<400>  2
taacttcaat tctaatattc ttgttcagaa cg                                     32


<210>  3
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  CF109 primer

<400>  3
caatcgtgct gaagaatctg aaagag                                            26


<210>  4
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  CF099 primer

<400>  4
ctgtagcaca agcctgattc gc                                                22


<210>  5
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  CF108  primer

<400>  5
tcccaagatg aaattgacgc g                                                 21


<210>  6
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  CF103 primer

<400>  6
ctactctttc accgatctga atggc                                             25


<210>  7
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  flaB4F primer

<400>  7
ctcatatttg cttgtgcgag c                                                 21


<210>  8
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  flaB4R primer

<400>  8
gaacgctact ggtttacaat tagttgc                                           27


<210>  9
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  rpoBF  primer

<400>  9
atggagcgga acgtgtagtc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  rpoBR primer

<400>  10
cttcgttcgt tccatgtcct                                                   20


<210>  11
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  FlimF primer

<400>  11
cgacacatat gacagaaatt ttat                                              24


<210>  12
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  FlimR2 primer

<400>  12
taacttcaat tctaatattc ttgttcagaa cg                                     32


<210>  13
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  fliM original sequence

<400>  13
gtttctgaac aaaaaaaagt aaagatctac gatttt                                 36


<210>  14
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  fliM mutated sequence

<400>  14
gtttctgaac aaaaaaagta aagatctacg atttta                                 36


<210>  15
<211>  1023
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA of wild-type fliM gene in strain Australis733 (WT)

<400>  15
atgacagaaa ttttatccca agatgaaatt gacgcgttac ttagcgccat cagttccggt       60

gaagtaagcg aatcggatta tgcttccgtt tctgaacaaa aaaaagtaaa gatctacgat      120

tttaaacgtc cggataaatt ttcaaaagac caaatccgta ctttacaaat gatgcatgaa      180

acctttgcac gtcttgcaac cacagggctt tctgctcagc taagagcgct tgtttcggtt      240

cacgttgctt ctgtggatca gttgacttac gaagagttca ttcgttccat tccaaatccc      300

acaacacttg cagtaatcaa catggaccct cttagaggtt ctgcaatctt agaaattgat      360

ccatcaattt cttttacgat catcgatcgt ctgtttggtg gtaaaggaga acaggcaaaa      420

atttccaggg aactttctga aatagaaatg agcgtaatgg aaggaattat tgtaagaatt      480

ttaggaaaca tgagagaatc gtggtccaca gtgatagact taagacctag gcttggaaac      540

attgaaacaa accctcaatt tgctcaagta gttcctccaa acgacatggt ggttttgatt      600

actctggaaa ctaaaatcgg agaagtggaa gggatgacga atctttgtat tccttatatc      660

acgatcgaac cgatcatcaa taaactatca gcacaatatt ggtattcttc cattcgtaag      720

ggagaattgg atgaaaaccg tgccgtgatt caggaaagat tggatcaagt agccattcct      780

ttgattgcgg aagttgggtc tgtggacgtt tccattaacg attttatgaa tctttctatt      840

ggagatgtag taaaactcga aaacacttct acaagatcag agatgatcgt aaaagtagga      900

gaaagaaaaa agttcaaatg ccttcctgga agagtaggaa gcagactcgc cattcagatc      960

ggtgaaagag tagaagatat tccagatgaa cttttgggtt ctactcgttc tgaacaagaa     1020

tat                                                                   1023


<210>  16
<211>  1022
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutated DNA of fliM gene in strain Australis702 (attenuated 
       strain)

<400>  16
atgacagaaa ttttatccca agatgaaatt gacgcgttac ttagcgccat cagttccggt       60

gaagtaagcg aatcggatta tgcttccgtt tctgaacaaa aaaagtaaag atctacgatt      120

ttaaacgtcc ggataaattt tcaaaagacc aaatccgtac tttacaaatg atgcatgaaa      180

cctttgcacg tcttgcaacc acagggcttt ctgctcagct aagagcgctt gtttcggttc      240

acgttgcttc tgtggatcag ttgacttacg aagagttcat tcgttccatt ccaaatccca      300

caacacttgc agtaatcaac atggaccctc ttagaggttc tgcaatctta gaaattgatc      360

catcaatttc ttttacgatc atcgatcgtc tgtttggtgg taaaggagaa caggcaaaaa      420

tttccaggga actttctgaa atagaaatga gcgtaatgga aggaattatt gtaagaattt      480

taggaaacat gagagaatcg tggtccacag tgatagactt aagacctagg cttggaaaca      540

ttgaaacaaa ccctcaattt gctcaagtag ttcctccaaa cgacatggtg gttttgatta      600

ctctggaaac taaaatcgga gaagtggaag ggatgacgaa tctttgtatt ccttatatca      660

cgatcgaacc gatcatcaat aaactatcag cacaatattg gtattcttcc attcgtaagg      720

gagaattgga tgaaaaccgt gccgtgattc aggaaagatt ggatcaagta gccattcctt      780

tgattgcgga agttgggtct gtggacgttt ccattaacga ttttatgaat ctttctattg      840

gagatgtagt aaaactcgaa aacacttcta caagatcaga gatgatcgta aaagtaggag      900

aaagaaaaaa gttcaaatgc cttcctggaa gagtaggaag cagactcgcc attcagatcg      960

gtgaaagagt agaagatatt ccagatgaac ttttgggttc tactcgttct gaacaagaat     1020

at                                                                    1022


<210>  17
<211>  341
<212>  PRT
<213>  artificial sequence

<220>
<223>  fliM protein translated from wild-type DNA of fliM in strain 
       Australis733 (WT)

<400>  17

Met Thr Glu Ile Leu Ser Gln Asp Glu Ile Asp Ala Leu Leu Ser Ala 
1               5                   10                  15      


Ile Ser Ser Gly Glu Val Ser Glu Ser Asp Tyr Ala Ser Val Ser Glu 
            20                  25                  30          


Gln Lys Lys Val Lys Ile Tyr Asp Phe Lys Arg Pro Asp Lys Phe Ser 
        35                  40                  45              


Lys Asp Gln Ile Arg Thr Leu Gln Met Met His Glu Thr Phe Ala Arg 
    50                  55                  60                  


Leu Ala Thr Thr Gly Leu Ser Ala Gln Leu Arg Ala Leu Val Ser Val 
65                  70                  75                  80  


His Val Ala Ser Val Asp Gln Leu Thr Tyr Glu Glu Phe Ile Arg Ser 
                85                  90                  95      


Ile Pro Asn Pro Thr Thr Leu Ala Val Ile Asn Met Asp Pro Leu Arg 
            100                 105                 110         


Gly Ser Ala Ile Leu Glu Ile Asp Pro Ser Ile Ser Phe Thr Ile Ile 
        115                 120                 125             


Asp Arg Leu Phe Gly Gly Lys Gly Glu Gln Ala Lys Ile Ser Arg Glu 
    130                 135                 140                 


Leu Ser Glu Ile Glu Met Ser Val Met Glu Gly Ile Ile Val Arg Ile 
145                 150                 155                 160 


Leu Gly Asn Met Arg Glu Ser Trp Ser Thr Val Ile Asp Leu Arg Pro 
                165                 170                 175     


Arg Leu Gly Asn Ile Glu Thr Asn Pro Gln Phe Ala Gln Val Val Pro 
            180                 185                 190         


Pro Asn Asp Met Val Val Leu Ile Thr Leu Glu Thr Lys Ile Gly Glu 
        195                 200                 205             


Val Glu Gly Met Thr Asn Leu Cys Ile Pro Tyr Ile Thr Ile Glu Pro 
    210                 215                 220                 


Ile Ile Asn Lys Leu Ser Ala Gln Tyr Trp Tyr Ser Ser Ile Arg Lys 
225                 230                 235                 240 


Gly Glu Leu Asp Glu Asn Arg Ala Val Ile Gln Glu Arg Leu Asp Gln 
                245                 250                 255     


Val Ala Ile Pro Leu Ile Ala Glu Val Gly Ser Val Asp Val Ser Ile 
            260                 265                 270         


Asn Asp Phe Met Asn Leu Ser Ile Gly Asp Val Val Lys Leu Glu Asn 
        275                 280                 285             


Thr Ser Thr Arg Ser Glu Met Ile Val Lys Val Gly Glu Arg Lys Lys 
    290                 295                 300                 


Phe Lys Cys Leu Pro Gly Arg Val Gly Ser Arg Leu Ala Ile Gln Ile 
305                 310                 315                 320 


Gly Glu Arg Val Glu Asp Ile Pro Asp Glu Leu Leu Gly Ser Thr Arg 
                325                 330                 335     


Ser Glu Gln Glu Tyr 
            340     


<210>  18
<211>  35
<212>  PRT
<213>  artificial sequence

<220>
<223>  truncated fliM protein translated from mutated DNA of fliM gene 
       in strain Australis702 (attenuated strain)

<400>  18

Met Thr Glu Ile Leu Ser Gln Asp Glu Ile Asp Ala Leu Leu Ser Ala 
1               5                   10                  15      


Ile Ser Ser Gly Glu Val Ser Glu Ser Asp Tyr Ala Ser Val Ser Glu 
            20                  25                  30          


Gln Lys Lys 
        35  


<210>  19
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Translation of fliM original sequence

<400>  19

Val Ser Glu Gln Lys Lys Val Lys Ile Tyr Asp Phe 
1               5                   10          


<210>  20
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  Translation of fliM mutated sequence

<400>  20

Val Ser Glu Gln Lys Lys 
1               5       


