                         SEQUENCE LISTING

<110>  Bristol-Myers Squibb Company
 
<120>  TRANSGLUTAMINASE VARIANTS WITH INCREASED SPECIFIC ACTIVITY

<130>  12614-WO-PCT

<150>  US 62/236282
<151>  2015-10-02

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  331
<212>  PRT
<213>  Streptomyces mobaraensis

<400>  1

Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met 
1               5                   10                  15      


Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn 
            20                  25                  30          


Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg 
        35                  40                  45              


Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Tyr Gly Cys 
    50                  55                  60                  


Val Gly Val Thr Trp Val Asn Ser Gly Gln Tyr Pro Thr Asn Arg Leu 
65                  70                  75                  80  


Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn 
                85                  90                  95      


Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val 
            100                 105                 110         


Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu 
        115                 120                 125             


Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser 
    130                 135                 140                 


Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn 
                165                 170                 175     


Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg 
            180                 185                 190         


Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg 
        195                 200                 205             


Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg 
    210                 215                 220                 


Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile 
225                 230                 235                 240 


Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr 
                245                 250                 255     


Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp 
            260                 265                 270         


Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met 
        275                 280                 285             


His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp 
    290                 295                 300                 


Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn 
305                 310                 315                 320 


Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro 
                325                 330     


<210>  2
<211>  382
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant transglutaminase proenzyme

<400>  2

Asp Asn Gly Ala Gly Glu Glu Thr Lys Ser Tyr Ala Glu Thr Tyr Arg 
1               5                   10                  15      


Leu Thr Ala Asp Asp Val Ala Asn Ile Asn Ala Leu Asn Glu Ser Ala 
            20                  25                  30          


Pro Ala Ala Ser Ser Ala Gly Pro Ser Phe Arg Ala Pro Asp Ser Asp 
        35                  40                  45              


Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met Pro Asp Pro 
    50                  55                  60                  


Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn Asn Tyr Ile 
65                  70                  75                  80  


Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg Lys Gln Gln 
                85                  90                  95      


Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Tyr Gly Cys Val Gly Val 
            100                 105                 110         


Thr Trp Val Asn Ser Gly Gln Tyr Pro Thr Asn Arg Leu Ala Phe Ala 
        115                 120                 125             


Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn Gly Arg Pro 
    130                 135                 140                 


Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val Ala Lys Glu 
145                 150                 155                 160 


Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu Val Ala Ser 
                165                 170                 175     


Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser Ala Tyr Leu 
            180                 185                 190         


Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala Leu Arg Asn 
        195                 200                 205             


Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn Thr Pro Ser 
    210                 215                 220                 


Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg Met Lys Ala 
225                 230                 235                 240 


Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg Ser Ser Ser 
                245                 250                 255     


Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg Pro Ala Pro 
            260                 265                 270         


Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile Pro Arg Ser 
        275                 280                 285             


Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr Gly Trp Phe 
    290                 295                 300                 


Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp Thr His Gly 
305                 310                 315                 320 


Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met His Val Tyr 
                325                 330                 335     


Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp Phe Asp Arg 
            340                 345                 350         


Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn Thr Ala Pro 
        355                 360                 365             


Asp Lys Val Lys Gln Gly Trp Pro His His His His His His 
    370                 375                 380         


<210>  3
<211>  341
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Activated recombinant transglutaminase


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  N-Terminal tetrapeptide

<220>
<221>  MISC_FEATURE
<222>  (336)..(341)
<223>  C-Terminal polyhistidine

<400>  3

Phe Arg Ala Pro Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro 
1               5                   10                  15      


Leu Asp Arg Met Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu 
            20                  25                  30          


Thr Val Val Asn Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His 
        35                  40                  45              


Arg Asp Gly Arg Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu 
    50                  55                  60                  


Ser Tyr Gly Cys Val Gly Val Thr Trp Val Asn Ser Gly Gln Tyr Pro 
65                  70                  75                  80  


Thr Asn Arg Leu Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn 
                85                  90                  95      


Glu Leu Lys Asn Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe 
            100                 105                 110         


Glu Gly Arg Val Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln 
        115                 120                 125             


Arg Ala Arg Glu Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala 
    130                 135                 140                 


His Asp Glu Ser Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn 
145                 150                 155                 160 


Gly Asn Asp Ala Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser 
                165                 170                 175     


Ala Leu Arg Asn Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His 
            180                 185                 190         


Asp Pro Ser Arg Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser 
        195                 200                 205             


Gly Gln Asp Arg Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro 
    210                 215                 220                 


Asp Ala Phe Arg Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg 
225                 230                 235                 240 


Asp Arg Asn Ile Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val 
                245                 250                 255     


Asn Phe Asp Tyr Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp 
            260                 265                 270         


Lys Thr Val Trp Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser 
        275                 280                 285             


Leu Gly Ala Met His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu 
    290                 295                 300                 


Gly Tyr Ser Asp Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro 
305                 310                 315                 320 


Lys Ser Trp Asn Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro His 
                325                 330                 335     


His His His His His 
            340     


<210>  4
<211>  331
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant M1


<220>
<221>  VARIANT
<222>  (65)..(65)
<223>  V65I substitution

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Y75Fsubstitution

<400>  4

Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met 
1               5                   10                  15      


Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn 
            20                  25                  30          


Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg 
        35                  40                  45              


Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Tyr Gly Cys 
    50                  55                  60                  


Ile Gly Val Thr Trp Val Asn Ser Gly Gln Phe Pro Thr Asn Arg Leu 
65                  70                  75                  80  


Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn 
                85                  90                  95      


Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val 
            100                 105                 110         


Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu 
        115                 120                 125             


Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser 
    130                 135                 140                 


Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn 
                165                 170                 175     


Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg 
            180                 185                 190         


Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg 
        195                 200                 205             


Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg 
    210                 215                 220                 


Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile 
225                 230                 235                 240 


Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr 
                245                 250                 255     


Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp 
            260                 265                 270         


Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met 
        275                 280                 285             


His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp 
    290                 295                 300                 


Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn 
305                 310                 315                 320 


Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro 
                325                 330     


<210>  5
<211>  331
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant M2


<220>
<221>  VARIANT
<222>  (62)..(62)
<223>  Y62H substitution

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Y75F substitution

<400>  5

Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met 
1               5                   10                  15      


Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn 
            20                  25                  30          


Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg 
        35                  40                  45              


Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Phe Gly Cys 
    50                  55                  60                  


Val Gly Val Thr Trp Val Asn Ser Gly Gln Phe Pro Thr Asn Arg Leu 
65                  70                  75                  80  


Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn 
                85                  90                  95      


Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val 
            100                 105                 110         


Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu 
        115                 120                 125             


Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser 
    130                 135                 140                 


Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn 
                165                 170                 175     


Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg 
            180                 185                 190         


Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg 
        195                 200                 205             


Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg 
    210                 215                 220                 


Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile 
225                 230                 235                 240 


Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr 
                245                 250                 255     


Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp 
            260                 265                 270         


Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met 
        275                 280                 285             


His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp 
    290                 295                 300                 


Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn 
305                 310                 315                 320 


Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro 
                325                 330     


<210>  6
<211>  331
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant M4


<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  Q74A substitution

<400>  6

Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met 
1               5                   10                  15      


Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn 
            20                  25                  30          


Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg 
        35                  40                  45              


Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Tyr Gly Cys 
    50                  55                  60                  


Val Gly Val Thr Trp Val Asn Ser Gly Ala Tyr Pro Thr Asn Arg Leu 
65                  70                  75                  80  


Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn 
                85                  90                  95      


Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val 
            100                 105                 110         


Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu 
        115                 120                 125             


Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser 
    130                 135                 140                 


Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn 
                165                 170                 175     


Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg 
            180                 185                 190         


Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg 
        195                 200                 205             


Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg 
    210                 215                 220                 


Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile 
225                 230                 235                 240 


Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr 
                245                 250                 255     


Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp 
            260                 265                 270         


Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met 
        275                 280                 285             


His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp 
    290                 295                 300                 


Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn 
305                 310                 315                 320 


Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro 
                325                 330     


<210>  7
<211>  331
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant M5


<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  W69A substitution

<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  Q74A substitution

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Y75F substitution

<400>  7

Asp Ser Asp Asp Arg Val Thr Pro Pro Ala Glu Pro Leu Asp Arg Met 
1               5                   10                  15      


Pro Asp Pro Tyr Arg Pro Ser Tyr Gly Arg Ala Glu Thr Val Val Asn 
            20                  25                  30          


Asn Tyr Ile Arg Lys Trp Gln Gln Val Tyr Ser His Arg Asp Gly Arg 
        35                  40                  45              


Lys Gln Gln Met Thr Glu Glu Gln Arg Glu Trp Leu Ser Tyr Gly Cys 
    50                  55                  60                  


Val Gly Val Thr Ala Val Asn Ser Gly Ala Phe Pro Thr Asn Arg Leu 
65                  70                  75                  80  


Ala Phe Ala Ser Phe Asp Glu Asp Arg Phe Lys Asn Glu Leu Lys Asn 
                85                  90                  95      


Gly Arg Pro Arg Ser Gly Glu Thr Arg Ala Glu Phe Glu Gly Arg Val 
            100                 105                 110         


Ala Lys Glu Ser Phe Asp Glu Glu Lys Gly Phe Gln Arg Ala Arg Glu 
        115                 120                 125             


Val Ala Ser Val Met Asn Arg Ala Leu Glu Asn Ala His Asp Glu Ser 
    130                 135                 140                 


Ala Tyr Leu Asp Asn Leu Lys Lys Glu Leu Ala Asn Gly Asn Asp Ala 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Ala Arg Ser Pro Phe Tyr Ser Ala Leu Arg Asn 
                165                 170                 175     


Thr Pro Ser Phe Lys Glu Arg Asn Gly Gly Asn His Asp Pro Ser Arg 
            180                 185                 190         


Met Lys Ala Val Ile Tyr Ser Lys His Phe Trp Ser Gly Gln Asp Arg 
        195                 200                 205             


Ser Ser Ser Ala Asp Lys Arg Lys Tyr Gly Asp Pro Asp Ala Phe Arg 
    210                 215                 220                 


Pro Ala Pro Gly Thr Gly Leu Val Asp Met Ser Arg Asp Arg Asn Ile 
225                 230                 235                 240 


Pro Arg Ser Pro Thr Ser Pro Gly Glu Gly Phe Val Asn Phe Asp Tyr 
                245                 250                 255     


Gly Trp Phe Gly Ala Gln Thr Glu Ala Asp Ala Asp Lys Thr Val Trp 
            260                 265                 270         


Thr His Gly Asn His Tyr His Ala Pro Asn Gly Ser Leu Gly Ala Met 
        275                 280                 285             


His Val Tyr Glu Ser Lys Phe Arg Asn Trp Ser Glu Gly Tyr Ser Asp 
    290                 295                 300                 


Phe Asp Arg Gly Ala Tyr Val Ile Thr Phe Ile Pro Lys Ser Trp Asn 
305                 310                 315                 320 


Thr Ala Pro Asp Lys Val Lys Gln Gly Trp Pro 
                325                 330     


<210>  8
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal tetrapeptide

<400>  8

Phe Arg Ala Pro 
1               


<210>  9
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer zg67.899

<400>  9
gcgctggctg gtttagtttt agcgtttagc gcatcggcgg ataacggcgc gggcgaagaa       60

ac                                                                      62


<210>  10
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer zg67,900

<400>  10
tctgtatcag gctgaaaatc ttatctcatc cgccaaaaca ttagtgatgg tgatggtgat       60

gtgaacccgg ccagccctgt ttcactttat c                                      91


<210>  11
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer zg67,903

<400>  11
tagaaataat tttgtttaac tttaagaagg agatatatat atggataatg gtgccggtga       60

agaaacca                                                                68


<210>  12
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer zg67,904

<400>  12
tctgtatcag gctgaaaatc ttatctcatc cgccaaaaca ttagtgatgg tgatggtgat       60

gtgaacccgg ccaaccctgt ttaactttat cc                                     92


<210>  13
<211>  1234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1 amplicon, periplasmic

<400>  13
gcgctggctg gtttagtttt agcgtttagc gcatcggcgg ataacggcgc gggcgaagaa       60

accaaaagct atgcggaaac ctatcgcctg accgcggatg atgtggcgaa cattaacgcg      120

ctgaacgaaa gcgcgccggc ggcgagcagc gcgggcccga gctttcgcgc gccggatagc      180

gatgatcgcg tgaccccgcc ggcggaaccg ctggatcgca tgccggatcc gtatcgcccg      240

agctatggcc gcgcggaaac cgtggtgaac aactatattc gcaaatggca gcaggtgtat      300

agccatcgcg atggccgcaa acagcagatg accgaagaac agcgcgaatg gctgagctat      360

ggctgcattg gcgtgacctg ggtgaacagc ggccagtttc cgaccaaccg cctggcgttt      420

gcgagctttg atgaagatcg ctttaaaaac gaactgaaaa acggccgccc gcgcagcggc      480

gaaacccgcg cggaatttga aggccgcgtg gcgaaagaaa gctttgatga agaaaaaggc      540

tttcagcgcg cgcgcgaagt ggcgagcgtg atgaaccgcg cgctggaaaa cgcgcatgat      600

gaaagcgcgt atctggataa cctgaaaaaa gaactggcga acggcaacga tgcgctgcgc      660

aacgaagatg cgcgcagccc gttttatagc gcgctgcgca acaccccgag ctttaaagaa      720

cgcaacggcg gcaaccatga tccgagccgc atgaaagcgg tgatttatag caaacatttt      780

tggagcggcc aggatcgcag cagcagcgcg gataaacgca aatatggcga tccggatgcg      840

tttcgcccgg cgccgggcac cggcctggtg gatatgagcc gcgatcgcaa cattccgcgc      900

agcccgacca gcccgggcga aggctttgtg aactttgatt atggctggtt tggcgcgcag      960

accgaagcgg atgcggataa aaccgtgtgg acccatggca accattatca tgcgccgaac     1020

ggcagcctgg gcgcgatgca tgtgtatgaa agcaaatttc gcaactggag cgaaggctat     1080

agcgattttg atcgcggcgc gtatgtgatt acctttattc cgaaaagctg gaacaccgcg     1140

ccggataaag tgaaacaggg ctggccgggt tcacatcacc atcaccatca ctaatgtttt     1200

ggcggatgag ataagatttt cagcctgata caga                                 1234


<210>  14
<211>  1238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1 amplicon, inclusion body

<400>  14
tagaaataat tttgtttaac tttaagaagg agatatatat atggataatg gtgccggtga       60

agaaaccaaa agctatgcag aaacctatcg tctgaccgca gatgatgttg caaacattaa      120

tgcactgaat gaaagcgcac cggcagcaag cagcgcaggt ccgagctttc gtgcaccgga      180

tagtgatgat cgtgttaccc ctccggcaga accgctggat cgtatgccgg atccgtatcg      240

tccgagctat ggtcgtgccg aaaccgttgt taataactat attcgtaaat ggcagcaggt      300

ctatagccat cgtgatggtc gtaaacagca gatgaccgaa gaacagcgtg aatggctgag      360

ttatggttgt attggtgtta cctgggttaa tagcggtcag tttccgacca atcgtctggc      420

atttgcaagc tttgatgaag atcgctttaa aaacgagctg aaaaatggtc gtccgcgtag      480

cggtgaaacc cgtgcagaat ttgaaggtcg tgttgcaaaa gaatccttcg atgaagaaaa      540

aggttttcag cgtgcccgtg aagttgcaag cgttatgaat cgtgcactgg aaaatgccca      600

tgatgaaagt gcatatctgg ataacctgaa aaaagaactg gccaatggta atgatgcact      660

gcgtaatgaa gatgcacgta gcccgtttta tagcgcactg cgcaataccc cgagctttaa      720

agaacgtaat ggtggtaatc atgatccgag ccgtatgaaa gcagtgatct atagcaaaca      780

tttttggagc ggtcaggatc gtagcagcag tgcagataaa cgtaaatatg gtgatccgga      840

tgcatttcgt ccggcaccgg gtacaggtct ggttgatatg agccgtgatc gtaatattcc      900

gcgtagtccg accagtccgg gtgaaggttt tgttaatttt gattatggtt ggtttggcgc      960

acagaccgaa gcagatgccg ataaaaccgt ttggacccat ggcaatcatt atcatgcacc     1020

gaatggtagc ctgggtgcaa tgcatgttta tgaaagtaaa tttcgcaatt ggagcgaggg     1080

ctatagcgat tttgatcgtg gtgcatatgt gattaccttt attccgaaaa gctggaatac     1140

cgctccggat aaagttaaac agggttggcc gggttcacat caccatcacc atcactaatg     1200

ttttggcgga tgagataaga ttttcagcct gatacaga                             1238


