﻿               SEQUENCE LISTING

<110> PARIS SCIENCES ET LETTRES - QUARTIER LATIN
      CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
      INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE
      TRAN VAN NHIEU Guy

<120> POLYPEPTIDES COMPRISING VINCULIN BINDING SITES FOR THE TREATMENT OF PROLIFERATION AND/OR ADHESION RELATED DISEASES

<130> CV - 524/PCT

<160> 79

<170> BiSSAP 1.3.6

<210> 1
<211> 633
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA (1-633)

<400> 1
Met His Asn Val Asn Asn Thr Gln Ala Pro Thr Phe Leu Tyr Lys Ala 
1               5                   10                  15      
Thr Ser Pro Ser Ser Thr Glu Tyr Ser Glu Leu Lys Ser Lys Ile Ser 
            20                  25                  30          
Asp Ile His Ser Ser Gln Thr Ser Leu Lys Thr Pro Ala Ser Val Ser 
        35                  40                  45              
Glu Lys Glu Asn Phe Ala Thr Ser Phe Asn Gln Lys Cys Leu Asp Phe 
    50                  55                  60                  
Leu Phe Ser Ser Ser Gly Lys Glu Asp Val Leu Arg Ser Ile Tyr Ser 
65                  70                  75                  80  
Asn Ser Met Asn Ala Tyr Ala Lys Ser Glu Ile Leu Glu Phe Ser Asn 
                85                  90                  95      
Val Leu Tyr Ser Leu Val His Gln Asn Gly Leu Asn Phe Glu Asn Glu 
            100                 105                 110         
Lys Gly Leu Gln Lys Ile Val Ala Gln Tyr Ser Glu Leu Ile Ile Lys 
        115                 120                 125             
Asp Lys Leu Ser Gln Asp Ser Ala Phe Gly Pro Trp Ser Ala Lys Asn 
    130                 135                 140                 
Lys Lys Leu His Gln Leu Arg Gln Asn Ile Glu His Arg Leu Ala Leu 
145                 150                 155                 160 
Leu Ala Gln Gln His Thr Ser Gly Glu Ala Leu Ser Leu Gly Gln Lys 
                165                 170                 175     
Leu Leu Asn Thr Glu Val Ser Ser Phe Ile Lys Asn Asn Ile Leu Ala 
            180                 185                 190         
Glu Leu Lys Leu Ser Asn Glu Thr Val Ser Ser Leu Lys Leu Asp Asp 
        195                 200                 205             
Leu Val Asp Ala Gln Ala Lys Leu Ala Phe Asp Ser Leu Arg Asn Gln 
    210                 215                 220                 
Arg Lys Asn Thr Ile Asp Ser Lys Gly Phe Gly Ile Gly Lys Leu Ser 
225                 230                 235                 240 
Arg Asp Leu Asn Thr Val Ala Val Phe Pro Glu Leu Leu Arg Lys Val 
                245                 250                 255     
Leu Asn Asp Ile Leu Glu Asp Ile Lys Asp Ser His Pro Ile Gln Asp 
            260                 265                 270         
Gly Leu Pro Thr Pro Pro Glu Asp Met Pro Asp Gly Gly Pro Thr Pro 
        275                 280                 285             
Gly Ala Asn Glu Lys Thr Ser Gln Pro Val Ile His Tyr His Ile Asn 
    290                 295                 300                 
Asn Asp Asn Arg Thr Tyr Asp Asn Arg Val Phe Asp Asn Arg Val Tyr 
305                 310                 315                 320 
Asp Asn Ser Tyr His Glu Asn Pro Glu Asn Asp Ala Gln Ser Pro Thr 
                325                 330                 335     
Ser Gln Thr Asn Asp Leu Leu Ser Arg Asn Gly Asn Ser Leu Leu Asn 
            340                 345                 350         
Pro Gln Arg Ala Leu Val Gln Lys Val Thr Ser Val Leu Pro His Ser 
        355                 360                 365             
Ile Ser Asp Thr Val Gln Thr Phe Ala Asn Asn Ser Ala Leu Glu Lys 
    370                 375                 380                 
Val Phe Asn His Thr Pro Asp Asn Ser Asp Gly Ile Gly Ser Asp Leu 
385                 390                 395                 400 
Leu Thr Thr Ser Ser Gln Glu Arg Ser Ala Asn Asn Ser Leu Ser Arg 
                405                 410                 415     
Gly His Arg Pro Leu Asn Ile Gln Asn Ser Ser Thr Thr Pro Pro Leu 
            420                 425                 430         
His Pro Glu Gly Val Thr Ser Ser Asn Asp Asn Ser Ser Asp Thr Thr 
        435                 440                 445             
Lys Ser Ser Ala Ser Leu Ser His Arg Val Ala Ser Gln Ile Asn Lys 
    450                 455                 460                 
Phe Asn Ser Asn Thr Asp Ser Lys Val Leu Gln Thr Asp Phe Leu Ser 
465                 470                 475                 480 
Arg Asn Gly Asp Thr Tyr Leu Thr Arg Glu Thr Ile Phe Glu Ala Ser 
                485                 490                 495     
Lys Lys Val Thr Asn Ser Leu Ser Asn Leu Ile Ser Leu Ile Gly Thr 
            500                 505                 510         
Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
        515                 520                 525             
Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Val Thr 
    530                 535                 540                 
Asp Ala Asn Ile Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
545                 550                 555                 560 
Asp Lys Asn His Ala Ile Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala 
                565                 570                 575     
Leu Ser Lys Val Leu Ser Lys Ile Asp Asp Thr Ser Ala Glu Leu Leu 
            580                 585                 590         
Thr Asp Asp Ile Ser Asp Leu Lys Asn Asn Asn Asp Ile Thr Ala Glu 
        595                 600                 605             
Asn Asn Asn Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser Leu Ser 
    610                 615                 620                 
Lys Val Leu Lys Asn Ile Asn Lys Asp 
625                 630             

<210> 2
<211> 19
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS1

<400> 2
Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser Leu Ser Lys Val Leu 
1               5                   10                  15      
Lys Asn Ile 
            

<210> 3
<211> 19
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS2

<400> 3
Ile Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala Leu Ser Lys Val Leu 
1               5                   10                  15      
Ser Lys Ile 
            

<210> 4
<211> 19
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS3

<400> 4
Ile Phe Glu Ala Ser Lys Lys Val Thr Asn Ser Leu Ser Asn Leu Ile 
1               5                   10                  15      
Ser Leu Ile 
            

<210> 5
<211> 151
<212> PRT
<213> Shigella flexneri


<220> 
<223> A483 / IpaA (483-633)

<400> 5
Gly Asp Thr Tyr Leu Thr Arg Glu Thr Ile Phe Glu Ala Ser Lys Lys 
1               5                   10                  15      
Val Thr Asn Ser Leu Ser Asn Leu Ile Ser Leu Ile Gly Thr Lys Ser 
            20                  25                  30          
Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile Thr Lys 
        35                  40                  45              
Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Val Thr Asp Ala 
    50                  55                  60                  
Asn Ile Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile Asp Lys 
65                  70                  75                  80  
Asn His Ala Ile Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala Leu Ser 
                85                  90                  95      
Lys Val Leu Ser Lys Ile Asp Asp Thr Ser Ala Glu Leu Leu Thr Asp 
            100                 105                 110         
Asp Ile Ser Asp Leu Lys Asn Asn Asn Asp Ile Thr Ala Glu Asn Asn 
        115                 120                 125             
Asn Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser Leu Ser Lys Val 
    130                 135                 140                 
Leu Lys Asn Ile Asn Lys Asp 
145                 150     

<210> 6
<211> 456
<212> DNA
<213> Shigella flexneri


<220> 
<223> A483 / IpaA (483-633)

<400> 6
ggagacacat atttaacacg ggaaacgata tttgaagctt caaaaaaagt aacaaactcc      60

ctaagtaatc ttatatctct cattggaact aaatcaggaa cacaagaacg agagttacag     120

gaaaaatcaa aggacattac aaaatccaca acagaacata gaataaacaa caaattaaaa     180

gttacagatg caaatataag aaactacgta acagaaacca acgcagatac aattgataaa     240

aatcatgcga tctatgaaaa ggcaaaagaa gtatctagcg ccctcagcaa ggtattgtca     300

aaaattgacg atacctctgc agaattactt acagatgata tatctgattt aaaaaataac     360

aatgatatta cagctgaaaa caataatata tataaagcag caaaagatgt aaccacttcc     420

ctatcaaaag tattaaagaa tatcaataag gattaa                               456


<210> 7
<211> 1902
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA (1-633)

<400> 7
atgcataatg taaataatac tcaagcgcca acattcttat ataaggcaac ttcaccatca      60

tcaacagaat acagcgagtt aaaaagcaaa atatccgata tccatagttc gcaaacttct     120

ctaaaaacac cagcatcagt gtctgaaaaa gaaaactttg caacgtcttt taatcagaaa     180

tgtcttgatt ttttattttc ttcctcaggg aaagaagatg tgttaagaag catttattcc     240

aactcaatga atgcgtatgc caaaagcgag attctcgaat tttcaaatgt tttgtactcc     300

ttagtacatc aaaatggtct taattttgaa aacgaaaagg gacttcaaaa aattgtcgca     360

cagtattcgg aactaattat aaaagataaa ttatcccaag attctgcctt tggaccatgg     420

tcggcaaaga ataagaaact ccatcaatta cgacaaaaca ttgagcacag acttgcacta     480

ttagcacaac aacacacatc tggtgaagct ttatcattgg gacaaaaact cctcaatact     540

gaagtatcat catttatcaa gaataatatt cttgctgaat taaagttaag taatgaaact     600

gtttcatctc tcaaactaga tgatttagtt gacgcacagg caaaacttgc ctttgatagt     660

ttgcgcaatc aacgtaaaaa tactattgat agtaaaggat ttggtatagg taaactgtca     720

agagacttaa atacagtagc cgtgtttcct gagctgttga gaaaagtcct taatgatatt     780

ttagaagata taaaagattc gcatcctatc caagatggcc tccctacacc tcccgaagat     840

atgccagatg gcggaccaac ccccggagcc aatgagaaaa catcccaacc tgtaattcac     900

tatcatataa ataatgataa tagaacttac gataatagag tttttgacaa cagagtatat     960

gacaatagct atcacgagaa cccagaaaat gatgcacagt ctcctacttc tcagacaaac    1020

gatctattat cccgtaacgg aaactcatta ctaaatccac aaagagcact agttcaaaaa    1080

gtaacttccg ttctaccaca ctctatatca gatactgtcc agacatttgc aaataattca    1140

gctttagaaa aggttttcaa ccatactcca gataattcgg atggaatagg ttcagacctg    1200

ttaactacga gtagtcaaga aagatctgca aataactctc tttctcgggg acacaggcct    1260

ctgaacatac agaactcttc aaccaccccc cctctccacc cggaaggagt gacaagcagt    1320

aatgataact catcagatac aactaaaagt agcgcttctc tttctcatag agtagcttcg    1380

caaatcaata aattcaactc aaacactgat tcaaaagtac ttcagactga ttttttatca    1440

agaaatggag acacatattt aacacgggaa acgatatttg aagcttcaaa aaaagtaaca    1500

aactccctaa gtaatcttat atctctcatt ggaactaaat caggaacaca agaacgagag    1560

ttacaggaaa aatcaaagga cattacaaaa tccacaacag aacatagaat aaacaacaaa    1620

ttaaaagtta cagatgcaaa tataagaaac tacgtaacag aaaccaacgc agatacaatt    1680

gataaaaatc atgcgatcta tgaaaaggca aaagaagtat ctagcgccct cagcaaggta    1740

ttgtcaaaaa ttgacgatac ctctgcagaa ttacttacag atgatatatc tgatttaaaa    1800

aataacaatg atattacagc tgaaaacaat aatatatata aagcagcaaa agatgtaacc    1860

acttccctat caaaagtatt aaagaatatc aataaggatt aa                       1902


<210> 8
<211> 57
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS1

<400> 8
atatataaag cagcaaaaga tgtaaccact tccctatcaa aagtattaaa gaatatc        57


<210> 9
<211> 57
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS2

<400> 9
atctatgaaa aggcaaaaga agtatctagc gccctcagca aggtattgtc aaaaatt        57


<210> 10
<211> 57
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS3

<400> 10
atatttgaag cttcaaaaaa agtaacaaac tccctaagta atcttatatc tctcatt        57


<210> 11
<211> 258
<212> PRT
<213> Homo sapiens


<220> 
<223> Vinculin domain D1 (HVD1)

<400> 11
Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile Leu Glu Pro Val 
1               5                   10                  15      
Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu Glu Gly Glu Val 
            20                  25                  30          
Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val Ala Ala Val Gln 
        35                  40                  45              
Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu Thr Val Gln Thr 
    50                  55                  60                  
Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro Ala Phe Ile Lys 
65                  70                  75                  80  
Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala Gln Met Leu Gln 
                85                  90                  95      
Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu Ile Asp Gly Ser 
            100                 105                 110         
Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu Thr Phe Asp Glu 
        115                 120                 125             
Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly Ile Leu Glu Tyr 
    130                 135                 140                 
Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp Leu Val Thr Tyr 
145                 150                 155                 160 
Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala Lys Met Ile Asp 
                165                 170                 175     
Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg Val Met Leu Val 
            180                 185                 190         
Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val Leu Ile Ser Ala 
        195                 200                 205             
Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn Gln Gly Ile Glu 
    210                 215                 220                 
Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys Met Ser Ala Glu 
225                 230                 235                 240 
Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser Trp Asp Glu Asp 
                245                 250                 255     
Ala Trp 
        

<210> 12
<211> 226
<212> PRT
<213> Homo sapiens


<220> 
<223> Vinculin domain D2 (HVD2)

<400> 12
Ala Ser Lys Asp Thr Glu Ala Met Lys Arg Ala Leu Ala Ser Ile Asp 
1               5                   10                  15      
Ser Lys Leu Asn Gln Ala Lys Gly Trp Leu Arg Asp Pro Ser Ala Ser 
            20                  25                  30          
Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg Gln Ile Leu Asp Glu Ala 
        35                  40                  45              
Gly Lys Val Gly Glu Leu Cys Ala Gly Lys Glu Arg Arg Glu Ile Leu 
    50                  55                  60                  
Gly Thr Cys Lys Met Leu Gly Gln Met Thr Asp Gln Val Ala Asp Leu 
65                  70                  75                  80  
Arg Ala Arg Gly Gln Gly Ser Ser Pro Val Ala Met Gln Lys Ala Gln 
                85                  90                  95      
Gln Val Ser Gln Gly Leu Asp Val Leu Thr Ala Lys Val Glu Asn Ala 
            100                 105                 110         
Ala Arg Lys Leu Glu Ala Met Thr Asn Ser Lys Gln Ser Ile Ala Lys 
        115                 120                 125             
Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala Asp Pro Asn Gly Gly Pro 
    130                 135                 140                 
Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu Ala Glu Ala Arg Lys Ile 
145                 150                 155                 160 
Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg Asp Asp Ile Leu Arg Ser 
                165                 170                 175     
Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys Leu Ala Asp Leu Arg Arg 
            180                 185                 190         
Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg Ala Leu Ala Lys Gln Val 
        195                 200                 205             
Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys Thr Asn Arg Ala Val Ala 
    210                 215                 220                 
Asn Ser 
225     

<210> 13
<211> 232
<212> PRT
<213> Homo sapiens


<220> 
<223> Vinculin domain D3 (HVD3)

<400> 13
Arg Pro Ala Lys Ala Ala Val His Leu Glu Gly Lys Ile Glu Gln Ala 
1               5                   10                  15      
Gln Arg Trp Ile Asp Asn Pro Thr Val Asp Asp Arg Gly Val Gly Gln 
            20                  25                  30          
Ala Ala Ile Arg Gly Leu Val Ala Glu Gly His Arg Leu Ala Asn Val 
        35                  40                  45              
Met Met Gly Pro Tyr Arg Gln Asp Leu Leu Ala Lys Cys Asp Arg Val 
    50                  55                  60                  
Asp Gln Leu Thr Ala Gln Leu Ala Asp Leu Ala Ala Arg Gly Glu Gly 
65                  70                  75                  80  
Glu Ser Pro Gln Ala Arg Ala Leu Ala Ser Gln Leu Gln Asp Ser Leu 
                85                  90                  95      
Lys Asp Leu Lys Ala Arg Met Gln Glu Ala Met Thr Gln Glu Val Ser 
            100                 105                 110         
Asp Val Phe Ser Asp Thr Thr Thr Pro Ile Lys Leu Leu Ala Val Ala 
        115                 120                 125             
Ala Thr Ala Pro Pro Asp Ala Pro Asn Arg Glu Glu Val Phe Asp Glu 
    130                 135                 140                 
Arg Ala Ala Asn Phe Glu Asn His Ser Gly Lys Leu Gly Ala Thr Ala 
145                 150                 155                 160 
Glu Lys Ala Ala Ala Val Gly Thr Ala Asn Lys Ser Thr Val Glu Gly 
                165                 170                 175     
Ile Gln Ala Ser Val Lys Thr Ala Arg Glu Leu Thr Pro Gln Val Val 
            180                 185                 190         
Ser Ala Ala Arg Ile Leu Leu Arg Asn Pro Gly Asn Gln Ala Ala Tyr 
        195                 200                 205             
Glu His Phe Glu Thr Met Lys Asn Gln Trp Ile Asp Asn Val Glu Lys 
    210                 215                 220                 
Met Thr Gly Leu Val Asp Glu Ala 
225                 230         

<210> 14
<211> 118
<212> PRT
<213> Homo sapiens


<220> 
<223> Vinculin domain D4 (HVD4)

<400> 14
Ile Asp Thr Lys Ser Leu Leu Asp Ala Ser Glu Glu Ala Ile Lys Lys 
1               5                   10                  15      
Asp Leu Asp Lys Cys Lys Val Ala Met Ala Asn Ile Gln Pro Gln Met 
            20                  25                  30          
Leu Val Ala Gly Ala Thr Ser Ile Ala Arg Arg Ala Asn Arg Ile Leu 
        35                  40                  45              
Leu Val Ala Lys Arg Glu Val Glu Asn Ser Glu Asp Pro Lys Phe Arg 
    50                  55                  60                  
Glu Ala Val Lys Ala Ala Ser Asp Glu Leu Ser Lys Thr Ile Ser Pro 
65                  70                  75                  80  
Met Val Met Asp Ala Lys Ala Val Ala Gly Asn Ile Ser Asp Pro Gly 
                85                  90                  95      
Leu Gln Lys Ser Phe Leu Asp Ser Gly Tyr Arg Ile Leu Gly Ala Val 
            100                 105                 110         
Ala Lys Val Arg Glu Ala 
        115             

<210> 15
<211> 110
<212> PRT
<213> Shigella flexneri


<220> 
<223> A524 / IpaA (524-633)

<400> 15
Lys Ser Lys Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn 
1               5                   10                  15      
Lys Leu Lys Val Thr Asp Ala Asn Ile Arg Asn Tyr Val Thr Glu Thr 
            20                  25                  30          
Asn Ala Asp Thr Ile Asp Lys Asn His Ala Ile Tyr Glu Lys Ala Lys 
        35                  40                  45              
Glu Val Ser Ser Ala Leu Ser Lys Val Leu Ser Lys Ile Asp Asp Thr 
    50                  55                  60                  
Ser Ala Glu Leu Leu Thr Asp Asp Ile Ser Asp Leu Lys Asn Asn Asn 
65                  70                  75                  80  
Asp Ile Thr Ala Glu Asn Asn Asn Ile Tyr Lys Ala Ala Lys Asp Val 
                85                  90                  95      
Thr Thr Ser Leu Ser Lys Val Leu Lys Asn Ile Asn Lys Asp 
            100                 105                 110 

<210> 16
<211> 3201
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human vinculin construct (HV)

<400> 16
atgccagtgt ttcatacgcg cacgatcgag agcatcctgg agccggtggc acagcagatc       60

tcccacctgg tgataatgca cgaggagggc gaggtggacg gcaaagccat tcctgacctc      120

accgcgcccg tggccgccgt gcaggcggcc gtcagcaacc tcgtccgggt tggaaaagag      180

actgttcaaa ccactgagga tcagattttg aagagagata tgccaccagc atttattaag      240

gttgagaatg cttgcaccaa gcttgtccag gcagctcaga tgcttcagtc agacccttac      300

tcagtgcctg ctcgagatta tctaattgat gggtcaaggg gcatcctctc tggaacatca      360

gacctgctcc ttaccttcga tgaggctgag gtccgtaaaa ttattagagt ttgcaaagga      420

attttggaat atcttacagt ggcagaggtg gtggagacta tggaagattt ggtcacttac      480

acaaagaatc ttgggccagg aatgactaag atggccaaga tgattgacga gagacagcag      540

gagctcactc accaggagca ccgagtgatg ttggtgaact cgatgaacac cgtgaaagag      600

ttgctgccag ttctcatttc agctatgaag atttttgtaa caactaaaaa ctcaaaaaac      660

caaggcatag aggaagcttt aaaaaatcgc aattttactg tagaaaaaat gagtgctgaa      720

attaatgaga taattcgtgt gttacaactc acctcttggg atgaagatgc ctgggccagc      780

aaggacactg aagccatgaa gagagcattg gcctccatag actccaaact gaaccaggcc      840

aaaggttggc tccgtgaccc tagtgcctcc ccaggggatg ctggtgagca ggccatcaga      900

cagatcttag atgaagctgg aaaagttggt gaactctgtg caggcaaaga acgcagggag      960

attctgggaa cttgcaaaat gctagggcag atgactgatc aagtggctga cctccgtgcc     1020

agaggacaag gatcctcacc ggtggccatg cagaaagctc agcaggtatc tcagggtctg     1080

gatgtgctca cagcaaaagt ggaaaatgca gctcgcaagc tggaagccat gaccaactca     1140

aagcagagca ttgcaaagaa gatcgatgct gctcagaact ggcttgcaga tccaaatggt     1200

ggaccggaag gagaagagca gattcgaggt gctttggctg aagctcggaa aatagcagaa     1260

ttatgtgatg atcctaaaga aagagatgac attctacgtt cccttgggga aatatctgct     1320

ctgacttcta aattagcaga tctacgaaga caggggaaag gagattctcc agaggctcga     1380

gccttggcca aacaggtggc cacggccctg cagaacctgc agaccaaaac caaccgggct     1440

gtggccaaca gcagaccggc caaagcagct gtacaccttg agggcaagat tgagcaagca     1500

cagcggtgga ttgataatcc cacagtggat gaccgtggag tcggtcaggc tgccatccgg     1560

gggcttgtgg ccgaagggca tcgtctggct aatgttatga tggggcctta tcggcaagat     1620

cttctcgcca agtgtgaccg agtggaccag ctgacagccc agctggctga cctggctgcc     1680

agaggggaag gggagagtcc tcaggcacga gcacttgcat ctcagctcca agactcctta     1740

aaggatctaa aagctcggat gcaggaggcc atgactcagg aagtgtcaga tgttttcagc     1800

gataccacaa ctcccatcaa gctgttggca gtggcagcca cggcgcctcc tgatgcgcct     1860

aacagggaag aggtatttga tgagagggca gctaactttg aaaaccattc aggaaagctt     1920

ggtgctacgg ccgagaaggc ggctgcggtt ggtactgcta ataaatcaac agtggaaggc     1980

attcaggcct cagtgaagac ggcccgagaa ctcacacccc aggtggtctc ggctgctcgt     2040

atcttactta ggaaccctgg aaatcaagct gcttatgaac attttgagac catgaagaac     2100

cagtggatcg ataatgttga aaaaatgaca gggctggtgg acgaagccat tgataccaaa     2160

tctctgttgg atgcttcaga agaagcaatt aaaaaagacc tggacaagtg caaggtagct     2220

atggccaaca ttcagcctca gatgctggtt gctggggcaa ccagtattgc tcgtcgggcc     2280

aaccggatcc tgctggtggc taagagggag gtggagaatt ccgaggatcc caagttccgt     2340

gaggctgtga aagctgcctc tgatgaattg agcaaaacca tctccccaat ggtgatggat     2400

gcaaaagctg tggctggaaa catttccgac cctggactgc aaaagagctt cctggactca     2460

ggatatcgga tcctgggagc tgtggccaag gtcagagaag ccttccaacc tcaggagcct     2520

gacttcccgc cgcctccacc agaccttgaa caactccgac taacagatga gcttgctcct     2580

cccaaaccac ctctgcctga aggtgaggtc cctccaccta ggcctccacc accagaggaa     2640

aaggatgaag agttccctga gcagaaggcc ggggaggtga ttaaccagcc aatgatgatg     2700

gctgccagac agctccatga tgaagctcgc aaatggtcca gcaagggcaa tgacatcatt     2760

gcagcagcca agcgcatggc tctgctgatg gctgagatgt ctcggctggt aagagggggc     2820

agtggtacca agcgggcact cattcagtgt gccaaggaca tcgccaaggc ctcagatgag     2880

gtgactcggt tggccaagga ggttgccaag cagtgcacag ataaacggat tagaaccaac     2940

ctcttacagg tatgtgagcg aatcccaacc ataagcaccc agctcaaaat cctgtccaca     3000

gtgaaggcca ccatgctggg ccggaccaac atcagtgatg aggagtctga gcaggccaca     3060

gagatgctgg ttcacaatgc ccagaacctc atgcagtctg tgaaggagac tgtgcgggaa     3120

gctgaagctg cttcaatcaa aattcgaaca gatgctggat ttacactgcg ctgggttaga     3180

aagactccct ggtaccagta g                                               3201


<210> 17
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV Fwd primer

<400> 17
gcgcatatgc cagtgtttca tacg                                           24


<210> 18
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV Rv primer

<400> 18
cgtcgactca ccaggcatct tcatc                                          25


<210> 19
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV Rv primer

<400> 19
cgtcgactca gtgtacagct gctttg                                         26


<210> 20
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV_K276R Fwd primer

<400> 20
gcattggcct ccatagactc ccgtctgaac caggccaaag g                         41


<210> 21
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV_K276R Rv primer

<400> 21
cctttggcct ggttcagacg ggagtctatg gaggccaatg c                         41


<210> 22
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV_E305Q Fwd primer

<400> 22
ggccatcaga cagatcttag atcaagctgg aaaagttggt g                         41


<210> 23
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> HV_E305Q Rv primer

<400> 23
caccaacttt tccagcttga tctaagatct gtctgatggc c                         41


<210> 24
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> GFP-IpaA483 Fwd primer

<400> 24
gcgatatcat ggccagcaaa gg                                             22


<210> 25
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> GFP-IpaA483 Rv primer

<400> 25
gcgcggccgc ttaatcctta ttgatattc                                      29


<210> 26
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> GST-IpaA483 Fwd primer

<400> 26
ggcgaattcc cggagacaca tatttaacac g                                   31


<210> 27
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> GST-IpaA483 Rv primer

<400> 27
gccgtcgact taatccttat tgatattct                                      29


<210> 28
<211> 1242
<212> DNA
<213> Artificial Sequence


<220> 
<223> GFP-IpaA483

<400> 28
atggccagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat      60

ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac     120

ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca     180

cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa     240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct     300

ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt     360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac     420

aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat     480

ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca     540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat     600

tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc     660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaagc     720

ggttccggac cggtgctagc ggtaccgagc tcggatccac tagtccagtg tggtggaatt     780

gcccttggag acacatattt aacacgggaa acgatatttg aagcttcaaa aaaagtaaca     840

aactccctaa gtaatcttat atctctcatt ggaactaaat caggaacaca agaacgagag     900

ttacaggaaa aatcaaagga cattacaaaa tccacaacag aacatagaat aaacaacaaa     960

ttaaaagtta cagatgcaaa tataagaaac tacgtaacag aaaccaacgc agatacaatt    1020

gataaaaatc atgcgatcta tgaaaaggca aaagaagtat ctagcgccct cagcaaggta    1080

ttgtcaaaaa ttgacgatac ctctgcagaa ttacttacag atgatatatc tgatttaaaa    1140

aataacaatg atattacagc tgaaaacaat aatatatata aagcagcaaa agatgtaacc    1200

acttccctat caaaagtatt aaagaatatc aataaggatt aa                       1242


<210> 29
<211> 1146
<212> DNA
<213> Artificial Sequence


<220> 
<223> GST-IpaA483

<400> 29
atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt      60

ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa     120

tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat     180

ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac     240

atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg     300

gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt     360

gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa     420

acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat     480

gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa     540

aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca     600

tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat     660

ctggttccgc gtggatcccc aggaattccc ggagacacat atttaacacg ggaaacgata     720

tttgaagctt caaaaaaagt aacaaactcc ctaagtaatc ttatatctct cattggaact     780

aaatcaggaa cacaagaacg agagttacag gaaaaatcaa aggacattac aaaatccaca     840

acagaacata gaataaacaa caaattaaaa gttacagatg caaatataag aaactacgta     900

acagaaacca acgcagatac aattgataaa aatcatgcga tctatgaaaa ggcaaaagaa     960

gtatctagcg ccctcagcaa ggtattgtca aaaattgacg atacctctgc agaattactt    1020

acagatgata tatctgattt aaaaaataac aatgatatta cagctgaaaa caataatata    1080

tataaagcag caaaagatgt aaccacttcc ctatcaaaag tattaaagaa tatcaataag    1140

gattaa                                                               1146


<210> 30
<211> 25
<212> RNA
<213> Artificial Sequence


<220> 
<223> anti-talin 1 siRNA

<400> 30
ccaagaacgg aaaccugcca gaguu                                          25


<210> 31
<211> 1066
<212> PRT
<213> Homo sapiens


<220> 
<223> Human vinculin (HV)

<400> 31
Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile Leu Glu Pro Val 
1               5                   10                  15      
Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu Glu Gly Glu Val 
            20                  25                  30          
Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val Ala Ala Val Gln 
        35                  40                  45              
Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu Thr Val Gln Thr 
    50                  55                  60                  
Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro Ala Phe Ile Lys 
65                  70                  75                  80  
Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala Gln Met Leu Gln 
                85                  90                  95      
Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu Ile Asp Gly Ser 
            100                 105                 110         
Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu Thr Phe Asp Glu 
        115                 120                 125             
Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly Ile Leu Glu Tyr 
    130                 135                 140                 
Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp Leu Val Thr Tyr 
145                 150                 155                 160 
Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala Lys Met Ile Asp 
                165                 170                 175     
Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg Val Met Leu Val 
            180                 185                 190         
Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val Leu Ile Ser Ala 
        195                 200                 205             
Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn Gln Gly Ile Glu 
    210                 215                 220                 
Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys Met Ser Ala Glu 
225                 230                 235                 240 
Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser Trp Asp Glu Asp 
                245                 250                 255     
Ala Trp Ala Ser Lys Asp Thr Glu Ala Met Lys Arg Ala Leu Ala Ser 
            260                 265                 270         
Ile Asp Ser Lys Leu Asn Gln Ala Lys Gly Trp Leu Arg Asp Pro Ser 
        275                 280                 285             
Ala Ser Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg Gln Ile Leu Asp 
    290                 295                 300                 
Glu Ala Gly Lys Val Gly Glu Leu Cys Ala Gly Lys Glu Arg Arg Glu 
305                 310                 315                 320 
Ile Leu Gly Thr Cys Lys Met Leu Gly Gln Met Thr Asp Gln Val Ala 
                325                 330                 335     
Asp Leu Arg Ala Arg Gly Gln Gly Ser Ser Pro Val Ala Met Gln Lys 
            340                 345                 350         
Ala Gln Gln Val Ser Gln Gly Leu Asp Val Leu Thr Ala Lys Val Glu 
        355                 360                 365             
Asn Ala Ala Arg Lys Leu Glu Ala Met Thr Asn Ser Lys Gln Ser Ile 
    370                 375                 380                 
Ala Lys Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala Asp Pro Asn Gly 
385                 390                 395                 400 
Gly Pro Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu Ala Glu Ala Arg 
                405                 410                 415     
Lys Ile Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg Asp Asp Ile Leu 
            420                 425                 430         
Arg Ser Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys Leu Ala Asp Leu 
        435                 440                 445             
Arg Arg Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg Ala Leu Ala Lys 
    450                 455                 460                 
Gln Val Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys Thr Asn Arg Ala 
465                 470                 475                 480 
Val Ala Asn Ser Arg Pro Ala Lys Ala Ala Val His Leu Glu Gly Lys 
                485                 490                 495     
Ile Glu Gln Ala Gln Arg Trp Ile Asp Asn Pro Thr Val Asp Asp Arg 
            500                 505                 510         
Gly Val Gly Gln Ala Ala Ile Arg Gly Leu Val Ala Glu Gly His Arg 
        515                 520                 525             
Leu Ala Asn Val Met Met Gly Pro Tyr Arg Gln Asp Leu Leu Ala Lys 
    530                 535                 540                 
Cys Asp Arg Val Asp Gln Leu Thr Ala Gln Leu Ala Asp Leu Ala Ala 
545                 550                 555                 560 
Arg Gly Glu Gly Glu Ser Pro Gln Ala Arg Ala Leu Ala Ser Gln Leu 
                565                 570                 575     
Gln Asp Ser Leu Lys Asp Leu Lys Ala Arg Met Gln Glu Ala Met Thr 
            580                 585                 590         
Gln Glu Val Ser Asp Val Phe Ser Asp Thr Thr Thr Pro Ile Lys Leu 
        595                 600                 605             
Leu Ala Val Ala Ala Thr Ala Pro Pro Asp Ala Pro Asn Arg Glu Glu 
    610                 615                 620                 
Val Phe Asp Glu Arg Ala Ala Asn Phe Glu Asn His Ser Gly Lys Leu 
625                 630                 635                 640 
Gly Ala Thr Ala Glu Lys Ala Ala Ala Val Gly Thr Ala Asn Lys Ser 
                645                 650                 655     
Thr Val Glu Gly Ile Gln Ala Ser Val Lys Thr Ala Arg Glu Leu Thr 
            660                 665                 670         
Pro Gln Val Val Ser Ala Ala Arg Ile Leu Leu Arg Asn Pro Gly Asn 
        675                 680                 685             
Gln Ala Ala Tyr Glu His Phe Glu Thr Met Lys Asn Gln Trp Ile Asp 
    690                 695                 700                 
Asn Val Glu Lys Met Thr Gly Leu Val Asp Glu Ala Ile Asp Thr Lys 
705                 710                 715                 720 
Ser Leu Leu Asp Ala Ser Glu Glu Ala Ile Lys Lys Asp Leu Asp Lys 
                725                 730                 735     
Cys Lys Val Ala Met Ala Asn Ile Gln Pro Gln Met Leu Val Ala Gly 
            740                 745                 750         
Ala Thr Ser Ile Ala Arg Arg Ala Asn Arg Ile Leu Leu Val Ala Lys 
        755                 760                 765             
Arg Glu Val Glu Asn Ser Glu Asp Pro Lys Phe Arg Glu Ala Val Lys 
    770                 775                 780                 
Ala Ala Ser Asp Glu Leu Ser Lys Thr Ile Ser Pro Met Val Met Asp 
785                 790                 795                 800 
Ala Lys Ala Val Ala Gly Asn Ile Ser Asp Pro Gly Leu Gln Lys Ser 
                805                 810                 815     
Phe Leu Asp Ser Gly Tyr Arg Ile Leu Gly Ala Val Ala Lys Val Arg 
            820                 825                 830         
Glu Ala Phe Gln Pro Gln Glu Pro Asp Phe Pro Pro Pro Pro Pro Asp 
        835                 840                 845             
Leu Glu Gln Leu Arg Leu Thr Asp Glu Leu Ala Pro Pro Lys Pro Pro 
    850                 855                 860                 
Leu Pro Glu Gly Glu Val Pro Pro Pro Arg Pro Pro Pro Pro Glu Glu 
865                 870                 875                 880 
Lys Asp Glu Glu Phe Pro Glu Gln Lys Ala Gly Glu Val Ile Asn Gln 
                885                 890                 895     
Pro Met Met Met Ala Ala Arg Gln Leu His Asp Glu Ala Arg Lys Trp 
            900                 905                 910         
Ser Ser Lys Gly Asn Asp Ile Ile Ala Ala Ala Lys Arg Met Ala Leu 
        915                 920                 925             
Leu Met Ala Glu Met Ser Arg Leu Val Arg Gly Gly Ser Gly Thr Lys 
    930                 935                 940                 
Arg Ala Leu Ile Gln Cys Ala Lys Asp Ile Ala Lys Ala Ser Asp Glu 
945                 950                 955                 960 
Val Thr Arg Leu Ala Lys Glu Val Ala Lys Gln Cys Thr Asp Lys Arg 
                965                 970                 975     
Ile Arg Thr Asn Leu Leu Gln Val Cys Glu Arg Ile Pro Thr Ile Ser 
            980                 985                 990         
Thr Gln Leu Lys Ile Leu Ser Thr Val Lys Ala Thr Met Leu Gly Arg 
        995                 1000                1005            
Thr Asn Ile Ser Asp Glu Glu Ser Glu Gln Ala Thr Glu Met Leu Val 
    1010                1015                1020                
His Asn Ala Gln Asn Leu Met Gln Ser Val Lys Glu Thr Val Arg Glu 
1025                1030                1035                1040
Ala Glu Ala Ala Ser Ile Lys Ile Arg Thr Asp Ala Gly Phe Thr Leu 
                1045                1050                1055    
Arg Trp Val Arg Lys Thr Pro Trp Tyr Gln 
            1060                1065    

<210> 32
<211> 333
<212> DNA
<213> Shigella flexneri


<220> 
<223> A524 / IpaA (524-633)

<400> 32
aaatcaaagg acattacaaa atccacaaca gaacatagaa taaacaacaa attaaaagtt      60

acagatgcaa atataagaaa ctacgtaaca gaaaccaacg cagatacaat tgataaaaat     120

catgcgatct atgaaaaggc aaaagaagta tctagcgccc tcagcaaggt attgtcaaaa     180

attgacgata cctctgcaga attacttaca gatgatatat ctgatttaaa aaataacaat     240

gatattacag ctgaaaacaa taatatatat aaagcagcaa aagatgtaac cacttcccta     300

tcaaaagtat taaagaatat caataaggat taa                                  333


<210> 33
<211> 837
<212> DNA
<213> Artificial Sequence


<220> 
<223> Vinculin domain D1 (HVD1)

<400> 33
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat      60

atgccagtgt ttcatacgcg cacgatcgag agcatcctgg agccggtggc acagcagatc     120

tcccacctgg tgataatgca cgaggagggc gaggtggacg gcaaagccat tcctgacctc     180

accgcgcccg tggccgccgt gcaggcggcc gtcagcaacc tcgtccgggt tggaaaagag     240

actgttcaaa ccactgagga tcagattttg aagagagata tgccaccagc atttattaag     300

gttgagaatg cttgcaccaa gcttgtccag gcagctcaga tgcttcagtc agacccttac     360

tcagtgcctg ctcgagatta tctaattgat gggtcaaggg gcatcctctc tggaacatca     420

gacctgctcc ttaccttcga tgaggctgag gtccgtaaaa ttattagagt ttgcaaagga     480

attttggaat atcttacagt ggcagaggtg gtggagacta tggaagattt ggtcacttac     540

acaaagaatc ttgggccagg aatgactaag atggccaaga tgattgacga gagacagcag     600

gagctcactc accaggagca ccgagtgatg ttggtgaact cgatgaacac cgtgaaagag     660

ttgctgccag ttctcatttc agctatgaag atttttgtaa caactaaaaa ctcaaaaaac     720

caaggcatag aggaagcttt aaaaaatcgc aattttactg tagaaaaaat gagtgctgaa     780

attaatgaga taattcgtgt gttacaactc acctcttggg atgaagatgc ctggtga        837


<210> 34
<211> 1539
<212> DNA
<213> Artificial Sequence


<220> 
<223> Vinculin domains D1-D2 (HVD1D2)

<400> 34
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat      60

atgccagtgt ttcatacgcg cacgatcgag agcatcctgg agccggtggc acagcagatc     120

tcccacctgg tgataatgca cgaggagggc gaggtggacg gcaaagccat tcctgacctc     180

accgcgcccg tggccgccgt gcaggcggcc gtcagcaacc tcgtccgggt tggaaaagag     240

actgttcaaa ccactgagga tcagattttg aagagagata tgccaccagc atttattaag     300

gttgagaatg cttgcaccaa gcttgtccag gcagctcaga tgcttcagtc agacccttac     360

tcagtgcctg ctcgagatta tctaattgat gggtcaaggg gcatcctctc tggaacatca     420

gacctgctcc ttaccttcga tgaggctgag gtccgtaaaa ttattagagt ttgcaaagga     480

attttggaat atcttacagt ggcagaggtg gtggagacta tggaagattt ggtcacttac     540

acaaagaatc ttgggccagg aatgactaag atggccaaga tgattgacga gagacagcag     600

gagctcactc accaggagca ccgagtgatg ttggtgaact cgatgaacac cgtgaaagag     660

ttgctgccag ttctcatttc agctatgaag atttttgtaa caactaaaaa ctcaaaaaac     720

caaggcatag aggaagcttt aaaaaatcgc aattttactg tagaaaaaat gagtgctgaa     780

attaatgaga taattcgtgt gttacaactc acctcttggg atgaagatgc ctgggccagc     840

aaggacactg aagccatgaa gagagcattg gcctccatag actccaaact gaaccaggcc     900

aaaggttggc tccgtgaccc tagtgcctcc ccaggggatg ctggtgagca ggccatcaga     960

cagatcttag atgaagctgg aaaagttggt gaactctgtg caggcaaaga acgcagggag    1020

attctgggaa cttgcaaaat gctagggcag atgactgatc aagtggctga cctccgtgcc    1080

agaggacaag gatcctcacc ggtggccatg cagaaagctc agcaggtatc tcagggtctg    1140

gatgtgctca cagcaaaagt ggaaaatgca gctcgcaagc tggaagccat gaccaactca    1200

aagcagagca ttgcaaagaa gatcgatgct gctcagaact ggcttgcaga tccaaatggt    1260

ggaccggaag gagaagagca gattcgaggt gctttggctg aagctcggaa aatagcagaa    1320

ttatgtgatg atcctaaaga aagagatgac attctacgtt cccttgggga aatatctgct    1380

ctgacttcta aattagcaga tctacgaaga caggggaaag gagattctcc agaggctcga    1440

gccttggcca aacaggtggc cacggccctg cagaacctgc agaccaaaac caaccgggct    1500

gtggccaaca gcagaccggc caaagcagct gtacactga                           1539


<210> 35
<211> 512
<212> PRT
<213> Artificial Sequence


<220> 
<223> Vinculin domains D1-D2 (HVD1D2)

<400> 35
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile 
            20                  25                  30          
Leu Glu Pro Val Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu 
        35                  40                  45              
Glu Gly Glu Val Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val 
    50                  55                  60                  
Ala Ala Val Gln Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu 
65                  70                  75                  80  
Thr Val Gln Thr Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro 
                85                  90                  95      
Ala Phe Ile Lys Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala 
            100                 105                 110         
Gln Met Leu Gln Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu 
        115                 120                 125             
Ile Asp Gly Ser Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu 
    130                 135                 140                 
Thr Phe Asp Glu Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly 
145                 150                 155                 160 
Ile Leu Glu Tyr Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp 
                165                 170                 175     
Leu Val Thr Tyr Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala 
            180                 185                 190         
Lys Met Ile Asp Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg 
        195                 200                 205             
Val Met Leu Val Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val 
    210                 215                 220                 
Leu Ile Ser Ala Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn 
225                 230                 235                 240 
Gln Gly Ile Glu Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys 
                245                 250                 255     
Met Ser Ala Glu Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser 
            260                 265                 270         
Trp Asp Glu Asp Ala Trp Ala Ser Lys Asp Thr Glu Ala Met Lys Arg 
        275                 280                 285             
Ala Leu Ala Ser Ile Asp Ser Lys Leu Asn Gln Ala Lys Gly Trp Leu 
    290                 295                 300                 
Arg Asp Pro Ser Ala Ser Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg 
305                 310                 315                 320 
Gln Ile Leu Asp Glu Ala Gly Lys Val Gly Glu Leu Cys Ala Gly Lys 
                325                 330                 335     
Glu Arg Arg Glu Ile Leu Gly Thr Cys Lys Met Leu Gly Gln Met Thr 
            340                 345                 350         
Asp Gln Val Ala Asp Leu Arg Ala Arg Gly Gln Gly Ser Ser Pro Val 
        355                 360                 365             
Ala Met Gln Lys Ala Gln Gln Val Ser Gln Gly Leu Asp Val Leu Thr 
    370                 375                 380                 
Ala Lys Val Glu Asn Ala Ala Arg Lys Leu Glu Ala Met Thr Asn Ser 
385                 390                 395                 400 
Lys Gln Ser Ile Ala Lys Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala 
                405                 410                 415     
Asp Pro Asn Gly Gly Pro Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu 
            420                 425                 430         
Ala Glu Ala Arg Lys Ile Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg 
        435                 440                 445             
Asp Asp Ile Leu Arg Ser Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys 
    450                 455                 460                 
Leu Ala Asp Leu Arg Arg Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg 
465                 470                 475                 480 
Ala Leu Ala Lys Gln Val Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys 
                485                 490                 495     
Thr Asn Arg Ala Val Ala Asn Ser Arg Pro Ala Lys Ala Ala Val His 
            500                 505                 510         



<210> 36
<211> 512
<212> PRT
<213> Artificial Sequence


<220> 
<223> Vinculin domains D1-D2 (HVD1D2) K276R

<400> 36
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile 
            20                  25                  30          
Leu Glu Pro Val Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu 
        35                  40                  45              
Glu Gly Glu Val Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val 
    50                  55                  60                  
Ala Ala Val Gln Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu 
65                  70                  75                  80  
Thr Val Gln Thr Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro 
                85                  90                  95      
Ala Phe Ile Lys Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala 
            100                 105                 110         
Gln Met Leu Gln Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu 
        115                 120                 125             
Ile Asp Gly Ser Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu 
    130                 135                 140                 
Thr Phe Asp Glu Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly 
145                 150                 155                 160 
Ile Leu Glu Tyr Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp 
                165                 170                 175     
Leu Val Thr Tyr Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala 
            180                 185                 190         
Lys Met Ile Asp Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg 
        195                 200                 205             
Val Met Leu Val Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val 
    210                 215                 220                 
Leu Ile Ser Ala Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn 
225                 230                 235                 240 
Gln Gly Ile Glu Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys 
                245                 250                 255     
Met Ser Ala Glu Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser 
            260                 265                 270         
Trp Asp Glu Asp Ala Trp Ala Ser Lys Asp Thr Glu Ala Met Lys Arg 
        275                 280                 285             
Ala Leu Ala Ser Ile Asp Ser Arg Leu Asn Gln Ala Lys Gly Trp Leu 
    290                 295                 300                 
Arg Asp Pro Ser Ala Ser Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg 
305                 310                 315                 320 
Gln Ile Leu Asp Glu Ala Gly Lys Val Gly Glu Leu Cys Ala Gly Lys 
                325                 330                 335     
Glu Arg Arg Glu Ile Leu Gly Thr Cys Lys Met Leu Gly Gln Met Thr 
            340                 345                 350         
Asp Gln Val Ala Asp Leu Arg Ala Arg Gly Gln Gly Ser Ser Pro Val 
        355                 360                 365             
Ala Met Gln Lys Ala Gln Gln Val Ser Gln Gly Leu Asp Val Leu Thr 
    370                 375                 380                 
Ala Lys Val Glu Asn Ala Ala Arg Lys Leu Glu Ala Met Thr Asn Ser 
385                 390                 395                 400 
Lys Gln Ser Ile Ala Lys Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala 
                405                 410                 415     
Asp Pro Asn Gly Gly Pro Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu 
            420                 425                 430         
Ala Glu Ala Arg Lys Ile Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg 
        435                 440                 445             
Asp Asp Ile Leu Arg Ser Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys 
    450                 455                 460                 
Leu Ala Asp Leu Arg Arg Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg 
465                 470                 475                 480 
Ala Leu Ala Lys Gln Val Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys 
                485                 490                 495     
Thr Asn Arg Ala Val Ala Asn Ser Arg Pro Ala Lys Ala Ala Val His 
            500                 505                 510         



<210> 37
<211> 512
<212> PRT
<213> Artificial Sequence


<220> 
<223> Vinculin domains D1-D2 (HVD1D2) E305Q

<400> 37
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Pro Val Phe His Thr Arg Thr Ile Glu Ser Ile 
            20                  25                  30          
Leu Glu Pro Val Ala Gln Gln Ile Ser His Leu Val Ile Met His Glu 
        35                  40                  45              
Glu Gly Glu Val Asp Gly Lys Ala Ile Pro Asp Leu Thr Ala Pro Val 
    50                  55                  60                  
Ala Ala Val Gln Ala Ala Val Ser Asn Leu Val Arg Val Gly Lys Glu 
65                  70                  75                  80  
Thr Val Gln Thr Thr Glu Asp Gln Ile Leu Lys Arg Asp Met Pro Pro 
                85                  90                  95      
Ala Phe Ile Lys Val Glu Asn Ala Cys Thr Lys Leu Val Gln Ala Ala 
            100                 105                 110         
Gln Met Leu Gln Ser Asp Pro Tyr Ser Val Pro Ala Arg Asp Tyr Leu 
        115                 120                 125             
Ile Asp Gly Ser Arg Gly Ile Leu Ser Gly Thr Ser Asp Leu Leu Leu 
    130                 135                 140                 
Thr Phe Asp Glu Ala Glu Val Arg Lys Ile Ile Arg Val Cys Lys Gly 
145                 150                 155                 160 
Ile Leu Glu Tyr Leu Thr Val Ala Glu Val Val Glu Thr Met Glu Asp 
                165                 170                 175     
Leu Val Thr Tyr Thr Lys Asn Leu Gly Pro Gly Met Thr Lys Met Ala 
            180                 185                 190         
Lys Met Ile Asp Glu Arg Gln Gln Glu Leu Thr His Gln Glu His Arg 
        195                 200                 205             
Val Met Leu Val Asn Ser Met Asn Thr Val Lys Glu Leu Leu Pro Val 
    210                 215                 220                 
Leu Ile Ser Ala Met Lys Ile Phe Val Thr Thr Lys Asn Ser Lys Asn 
225                 230                 235                 240 
Gln Gly Ile Glu Glu Ala Leu Lys Asn Arg Asn Phe Thr Val Glu Lys 
                245                 250                 255     
Met Ser Ala Glu Ile Asn Glu Ile Ile Arg Val Leu Gln Leu Thr Ser 
            260                 265                 270         
Trp Asp Glu Asp Ala Trp Ala Ser Lys Asp Thr Glu Ala Met Lys Arg 
        275                 280                 285             
Ala Leu Ala Ser Ile Asp Ser Lys Leu Asn Gln Ala Lys Gly Trp Leu 
    290                 295                 300                 
Arg Asp Pro Ser Ala Ser Pro Gly Asp Ala Gly Glu Gln Ala Ile Arg 
305                 310                 315                 320 
Gln Ile Leu Asp Gln Ala Gly Lys Val Gly Glu Leu Cys Ala Gly Lys 
                325                 330                 335     
Glu Arg Arg Glu Ile Leu Gly Thr Cys Lys Met Leu Gly Gln Met Thr 
            340                 345                 350         
Asp Gln Val Ala Asp Leu Arg Ala Arg Gly Gln Gly Ser Ser Pro Val 
        355                 360                 365             
Ala Met Gln Lys Ala Gln Gln Val Ser Gln Gly Leu Asp Val Leu Thr 
    370                 375                 380                 
Ala Lys Val Glu Asn Ala Ala Arg Lys Leu Glu Ala Met Thr Asn Ser 
385                 390                 395                 400 
Lys Gln Ser Ile Ala Lys Lys Ile Asp Ala Ala Gln Asn Trp Leu Ala 
                405                 410                 415     
Asp Pro Asn Gly Gly Pro Glu Gly Glu Glu Gln Ile Arg Gly Ala Leu 
            420                 425                 430         
Ala Glu Ala Arg Lys Ile Ala Glu Leu Cys Asp Asp Pro Lys Glu Arg 
        435                 440                 445             
Asp Asp Ile Leu Arg Ser Leu Gly Glu Ile Ser Ala Leu Thr Ser Lys 
    450                 455                 460                 
Leu Ala Asp Leu Arg Arg Gln Gly Lys Gly Asp Ser Pro Glu Ala Arg 
465                 470                 475                 480 
Ala Leu Ala Lys Gln Val Ala Thr Ala Leu Gln Asn Leu Gln Thr Lys 
                485                 490                 495     
Thr Asn Arg Ala Val Ala Asn Ser Arg Pro Ala Lys Ala Ala Val His 
            500                 505                 510         



<210> 38
<211> 155
<212> PRT
<213> Homo sapiens


<220> 
<223> Talin-1 VBS1 (482-636)

<400> 38
Arg Gly His Met Pro Pro Leu Thr Ser Ala Gln Gln Ala Leu Thr Gly 
1               5                   10                  15      
Thr Ile Asn Ser Ser Met Gln Ala Val Gln Ala Ala Gln Ala Thr Leu 
            20                  25                  30          
Asp Asp Phe Asp Thr Leu Pro Pro Leu Gly Gln Asp Ala Ala Ser Lys 
        35                  40                  45              
Ala Trp Arg Lys Asn Lys Met Asp Glu Ser Lys His Glu Ile His Ser 
    50                  55                  60                  
Gln Val Asp Ala Ile Thr Ala Gly Thr Ala Ser Val Val Asn Leu Thr 
65                  70                  75                  80  
Ala Gly Asp Pro Ala Glu Thr Asp Tyr Thr Ala Val Gly Cys Ala Val 
                85                  90                  95      
Thr Thr Ile Ser Ser Asn Leu Thr Glu Met Ser Arg Gly Val Lys Leu 
            100                 105                 110         
Leu Ala Ala Leu Leu Glu Asp Glu Gly Gly Ser Gly Arg Pro Leu Leu 
        115                 120                 125             
Gln Ala Ala Lys Gly Leu Ala Gly Ala Val Ser Glu Leu Leu Arg Ser 
    130                 135                 140                 
Ala Gln Pro Ala Ser Ala Glu Pro Arg Gln Asn 
145                 150                 155 

<210> 39
<211> 26
<212> PRT
<213> Homo sapiens


<220> 
<223> Talin-1 VBS2 (1944-1969)

<400> 39
Ala Tyr Thr Lys Lys Glu Leu Ile Glu Cys Ala Arg Arg Val Ser Glu 
1               5                   10                  15      
Lys Val Ser His Val Leu Ala Ala Leu Gln 
            20                  25      

<210> 40
<211> 9
<212> PRT
<213> Shigella flexneri


<220> 
<223> N-ter Domain

<400> 40
Gly Asp Thr Tyr Leu Thr Arg Glu Thr 
1               5                   

<210> 41
<211> 55
<212> PRT
<213> Shigella flexneri


<220> 
<223> Interdomain VBS3-VBS2

<400> 41
Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys 
1               5                   10                  15      
Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys 
            20                  25                  30          
Val Thr Asp Ala Asn Ile Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp 
        35                  40                  45              
Thr Ile Asp Lys Asn His Ala 
    50                  55  

<210> 42
<211> 27
<212> PRT
<213> Shigella flexneri


<220> 
<223> Interdomain VBS2-VBS1

<400> 42
Asp Asp Thr Ser Ala Glu Leu Leu Thr Asp Asp Ile Ser Asp Leu Lys 
1               5                   10                  15      
Asn Asn Asn Asp Ile Thr Ala Glu Asn Asn Asn 
            20                  25          

<210> 43
<211> 481
<212> PRT
<213> Shigella sonnei


<220> 
<223> SipA

<400> 43
Asn Ile Glu His Arg Leu Ala Leu Leu Ala Gln Gln His Thr Ser Gly 
1               5                   10                  15      
Glu Ala Leu Ser Leu Gly Gln Lys Leu Leu Asn Thr Glu Val Ser Ser 
            20                  25                  30          
Phe Ile Lys Asn Asn Ile Leu Ala Glu Leu Lys Leu Ser Asn Glu Thr 
        35                  40                  45              
Val Ser Ser Leu Lys Leu Asp Asp Leu Val Asp Ala Gln Ala Lys Leu 
    50                  55                  60                  
Ala Phe Asp Ser Leu Arg Asn Gln Arg Lys Asn Thr Ile Asp Ser Lys 
65                  70                  75                  80  
Gly Phe Gly Ile Gly Lys Leu Ser Arg Asp Leu Asn Thr Val Ala Val 
                85                  90                  95      
Phe Pro Glu Leu Leu Arg Lys Val Leu Asn Asp Ile Leu Glu Asp Ile 
            100                 105                 110         
Lys Asp Ser His Pro Ile Gln Asp Gly Leu Pro Thr Pro Pro Glu Asp 
        115                 120                 125             
Met Pro Asp Gly Gly Pro Thr Pro Gly Ala Asn Glu Lys Thr Ser Gln 
    130                 135                 140                 
Pro Val Ile His Tyr His Ile Asn Asn Asp Asn Arg Thr Tyr Asp Asn 
145                 150                 155                 160 
Arg Val Phe Asp Asn Arg Val Tyr Asp Asn Ser Tyr His Glu Asn Pro 
                165                 170                 175     
Glu Asn Asp Ala Gln Ser Pro Thr Ser Gln Thr Asn Asp Leu Leu Ser 
            180                 185                 190         
Arg Asn Gly Asn Ser Leu Leu Asn Pro Gln Arg Ala Leu Val Gln Lys 
        195                 200                 205             
Val Thr Ser Val Leu Pro His Ser Ile Ser Asp Thr Val Gln Thr Phe 
    210                 215                 220                 
Ala Asn Asn Ser Ala Leu Glu Lys Val Phe Asn His Thr Pro Asp Asn 
225                 230                 235                 240 
Ser Asp Gly Ile Gly Ser Asp Leu Leu Thr Thr Ser Ser Gln Glu Arg 
                245                 250                 255     
Ser Ala Asn Asn Ser Leu Ser Arg Gly His Arg Pro Leu Asn Ile Gln 
            260                 265                 270         
Asn Ser Ser Thr Thr Pro Pro Leu His Pro Glu Gly Val Thr Ser Ser 
        275                 280                 285             
Asn Asp Asn Ser Ser Asp Thr Thr Lys Ser Ser Ala Ser Leu Ser His 
    290                 295                 300                 
Arg Val Ala Ser Gln Ile Asn Lys Phe Asn Ser Asn Thr Asp Ser Lys 
305                 310                 315                 320 
Val Leu Gln Thr Asp Phe Phe Ser Arg Asn Gly Asp Thr Tyr Leu Thr 
                325                 330                 335     
Arg Glu Thr Ile Phe Glu Ala Ser Lys Lys Val Thr Asn Ser Leu Ser 
            340                 345                 350         
Asn Leu Ile Ser Leu Ile Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu 
        355                 360                 365             
Leu Gln Glu Lys Ser Lys Asp Ile Thr Lys Ser Thr Thr Glu His Arg 
    370                 375                 380                 
Ile Asn Asn Lys Leu Lys Val Thr Asp Ala Asn Thr Ile Asn Tyr Val 
385                 390                 395                 400 
Thr Glu Thr Asn Ala Asp Thr Ile Asp Lys Asn His Ala Ile Tyr Glu 
                405                 410                 415     
Lys Ala Lys Glu Val Ser Ser Ala Leu Ser Lys Val Leu Ser Lys Ile 
            420                 425                 430         
Asp Asp Thr Ser Ala Glu Leu Leu Thr Asp Asp Ile Ser Asp Leu Lys 
        435                 440                 445             
Asn Asn Asn Asp Ile Thr Ala Glu Asn Asn Asn Ile Tyr Lys Ala Ala 
    450                 455                 460                 
Lys Asp Val Thr Thr Ser Leu Ser Lys Val Leu Lys Asn Ile Asn Lys 
465                 470                 475                 480 
Asp 
    

<210> 44
<211> 500
<212> PRT
<213> Shigella sonnei


<220> 
<223> SipA

<400> 44
Val Ala Gln Tyr Ser Glu Leu Ile Ile Lys Asp Lys Leu Ser Gln Asp 
1               5                   10                  15      
Ser Ala Phe Gly Pro Trp Ser Ala Lys Asn Lys Lys Leu His Gln Leu 
            20                  25                  30          
Arg Gln Asn Ile Glu His Arg Leu Ala Leu Leu Ala Gln Gln His Thr 
        35                  40                  45              
Ser Gly Glu Ala Leu Ser Leu Gly Gln Lys Leu Leu Asn Thr Glu Val 
    50                  55                  60                  
Ser Ser Phe Ile Lys Asn Asn Ile Leu Ala Glu Leu Lys Leu Ser Asn 
65                  70                  75                  80  
Glu Thr Val Ser Ser Leu Lys Leu Asp Asp Leu Val Asp Ala Gln Ala 
                85                  90                  95      
Lys Leu Ala Phe Asp Ser Leu Arg Asn Gln Arg Lys Asn Thr Ile Asp 
            100                 105                 110         
Ser Lys Gly Phe Gly Ile Gly Lys Leu Ser Arg Asp Leu Asn Thr Val 
        115                 120                 125             
Ala Val Phe Pro Glu Leu Leu Arg Lys Val Leu Asn Asp Ile Leu Glu 
    130                 135                 140                 
Asp Ile Lys Asp Ser His Pro Ile Gln Asp Gly Leu Pro Thr Pro Pro 
145                 150                 155                 160 
Glu Asp Met Pro Asp Gly Gly Pro Thr Pro Gly Ala Asn Glu Lys Thr 
                165                 170                 175     
Ser Gln Pro Val Ile His Tyr His Ile Asn Asn Asp Asn Arg Thr Tyr 
            180                 185                 190         
Asp Asn Arg Val Phe Asp Asn Arg Val Tyr Asp Asn Ser Tyr His Glu 
        195                 200                 205             
Asn Pro Glu Asn Asp Ala Gln Ser Pro Thr Ser Gln Thr Asn Asp Leu 
    210                 215                 220                 
Leu Ser Arg Asn Gly Asn Ser Leu Leu Asn Pro Gln Arg Ala Leu Val 
225                 230                 235                 240 
Gln Lys Val Thr Ser Val Leu Pro His Ser Ile Ser Asp Thr Val Gln 
                245                 250                 255     
Thr Phe Ala Asn Asn Ser Ala Leu Glu Lys Val Phe Asn His Thr Pro 
            260                 265                 270         
Asp Asn Ser Asp Gly Ile Gly Ser Asp Leu Leu Thr Thr Ser Ser Gln 
        275                 280                 285             
Glu Arg Ser Ala Asn Asn Ser Leu Ser Arg Gly His Arg Pro Leu Asn 
    290                 295                 300                 
Ile Gln Asn Ser Ser Thr Thr Pro Pro Leu His Pro Glu Gly Val Thr 
305                 310                 315                 320 
Ser Ser Asn Asp Asn Ser Ser Asp Thr Thr Lys Ser Ser Ala Ser Leu 
                325                 330                 335     
Ser His Arg Val Ala Ser Gln Ile Asn Lys Phe Asn Ser Asn Thr Asp 
            340                 345                 350         
Ser Lys Val Leu Gln Thr Asp Phe Phe Ser Arg Asn Gly Asp Thr Tyr 
        355                 360                 365             
Leu Thr Arg Glu Thr Ile Phe Glu Ala Ser Lys Lys Val Thr Asn Ser 
    370                 375                 380                 
Leu Ser Asn Leu Ile Ser Leu Ile Gly Thr Lys Ser Gly Thr Gln Glu 
385                 390                 395                 400 
Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile Thr Lys Ser Thr Thr Glu 
                405                 410                 415     
His Arg Ile Asn Asn Lys Leu Lys Val Thr Asp Ala Asn Thr Ile Asn 
            420                 425                 430         
Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile Asp Lys Asn His Ala Ile 
        435                 440                 445             
Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala Leu Ser Lys Val Leu Ser 
    450                 455                 460                 
Lys Ile Asp Asp Thr Ser Ala Glu Leu Leu Thr Asp Asp Ile Ser Asp 
465                 470                 475                 480 
Leu Lys Asn Asn Asn Asp Ile Thr Ala Glu Asn Asn Asn Ile Tyr Lys 
                485                 490                 495     
Ala Ala Lys Asp 
            500 

<210> 45
<211> 587
<212> PRT
<213> Shigella sonnei 53G


<220> 
<223> YopE

<400> 45
Met Ser Glu Lys Glu Ser Phe Ala Thr Ser Phe Asn Gln Lys Cys Leu 
1               5                   10                  15      
Asp Phe Leu Phe Ser Ser Ser Gly Lys Glu Asp Val Leu Arg Ser Ile 
            20                  25                  30          
Tyr Ser Asn Ser Met Asn Ala Tyr Ala Lys Ser Glu Ile Leu Glu Phe 
        35                  40                  45              
Ser Asn Val Leu Tyr Ser Leu Val His Gln Asn Gly Leu Asn Phe Glu 
    50                  55                  60                  
Asn Glu Lys Gly Leu Gln Lys Ile Val Ala Gln Tyr Ser Glu Leu Ile 
65                  70                  75                  80  
Ile Lys Asp Lys Leu Ser Gln Asp Ser Ala Phe Gly Pro Trp Ser Ala 
                85                  90                  95      
Lys Asn Lys Lys Leu His Gln Leu Arg Gln Asn Ile Glu His Arg Leu 
            100                 105                 110         
Ala Leu Leu Ala Gln Gln His Thr Ser Gly Glu Ala Leu Ser Leu Gly 
        115                 120                 125             
Gln Lys Leu Leu Asn Thr Glu Val Ser Ser Phe Ile Lys Asn Asn Ile 
    130                 135                 140                 
Leu Ala Glu Leu Lys Leu Ser Asn Glu Thr Val Ser Ser Leu Lys Leu 
145                 150                 155                 160 
Asp Asp Leu Val Asp Ala Gln Ala Lys Leu Ala Phe Asp Ser Leu Arg 
                165                 170                 175     
Asn Gln Arg Lys Asn Thr Ile Asp Ser Lys Gly Phe Gly Ile Gly Lys 
            180                 185                 190         
Leu Ser Arg Asp Leu Asn Thr Val Ala Val Phe Pro Glu Leu Leu Arg 
        195                 200                 205             
Lys Val Leu Asn Asp Ile Leu Glu Asp Ile Lys Asp Ser His Pro Ile 
    210                 215                 220                 
Gln Asp Gly Leu Pro Thr Pro Pro Glu Asp Met Pro Asp Gly Gly Pro 
225                 230                 235                 240 
Thr Pro Gly Ala Asn Glu Lys Thr Ser Gln Pro Val Ile His Tyr His 
                245                 250                 255     
Ile Asn Asn Asp Asn Arg Thr Tyr Asp Asn Arg Val Phe Asp Asn Arg 
            260                 265                 270         
Val Tyr Asp Asn Ser Tyr His Glu Asn Pro Glu Asn Asp Ala Gln Ser 
        275                 280                 285             
Pro Thr Ser Gln Thr Asn Asp Leu Leu Ser Arg Asn Gly Asn Ser Leu 
    290                 295                 300                 
Leu Asn Pro Gln Arg Ala Leu Val Gln Lys Val Thr Ser Val Leu Pro 
305                 310                 315                 320 
His Ser Ile Ser Asp Thr Val Gln Thr Phe Ala Asn Asn Ser Ala Leu 
                325                 330                 335     
Glu Lys Val Phe Asn His Thr Pro Asp Asn Ser Asp Gly Ile Gly Ser 
            340                 345                 350         
Asp Leu Leu Thr Thr Ser Ser Gln Glu Arg Ser Ala Asn Asn Ser Leu 
        355                 360                 365             
Ser Arg Gly His Arg Pro Leu Asn Ile Gln Asn Ser Ser Thr Thr Pro 
    370                 375                 380                 
Pro Leu His Pro Glu Gly Val Thr Ser Ser Asn Asp Asn Ser Ser Asp 
385                 390                 395                 400 
Thr Thr Lys Ser Ser Ala Ser Leu Ser His Arg Val Ala Ser Gln Ile 
                405                 410                 415     
Asn Lys Phe Asn Ser Asn Thr Asp Ser Lys Val Leu Gln Thr Asp Phe 
            420                 425                 430         
Phe Ser Arg Asn Gly Asp Thr Tyr Leu Thr Arg Glu Thr Ile Phe Glu 
        435                 440                 445             
Ala Ser Lys Lys Val Thr Asn Ser Leu Ser Asn Leu Ile Ser Leu Ile 
    450                 455                 460                 
Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys 
465                 470                 475                 480 
Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys 
                485                 490                 495     
Val Thr Asp Ala Asn Thr Ile Asn Tyr Val Thr Glu Thr Asn Ala Asp 
            500                 505                 510         
Thr Ile Asp Lys Asn His Ala Ile Tyr Glu Lys Ala Lys Glu Val Ser 
        515                 520                 525             
Ser Ala Leu Ser Lys Val Leu Ser Lys Ile Asp Asp Thr Ser Ala Glu 
    530                 535                 540                 
Leu Leu Thr Asp Asp Ile Ser Asp Leu Lys Asn Asn Asn Asp Ile Thr 
545                 550                 555                 560 
Ala Glu Asn Asn Asn Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser 
                565                 570                 575     
Leu Ser Lys Val Leu Lys Asn Ile Asn Lys Asp 
            580                 585         

<210> 46
<211> 1902
<212> DNA
<213> Shigella flexneri 6


<220> 
<223> pINV_F6_M1382 (3109-5010)

<400> 46
ttaatcctta ttgatattct ttaatacttt tgatagggaa gtggttacat cttttgctgc      60

tttatatata ttattgtttt cagctgtaat atcattgtta ttttttaaat cagatatatc     120

atctgtaagt aattctgcag aggtatcgtc aatttttgac aataccttgc tgagggcgct     180

agatacttct tttgcctttt catagatcgc atgattttta tcaattgtat ctgcgttggt     240

ttctgttacg tagtttattg tatttgcatc tgtaactttt aatttgttgt ttattctgtg     300

ttctgttgtg gattttgtaa tgtcctttga tttttcctgt aactctcgtt cttgtgttcc     360

tgatttagtt ccaatgagag atataagatt acttagggag tttgttactt tttttgaagc     420

ttcaaatatc gtttcccgtg ttaaatatgt gtctccattt cttgataaaa aatcagtctg     480

aagtactttt gaatcagtgt ttgagttgaa tttattgatt tgcgaagcta ctctatgaga     540

aagagaagcg ctacttttag ttgtatctga tgagttatca ttactgcttg tcactccttc     600

cgggtggaga gggggggtgg ttgaagagtt ctgtatgttc agaggcctgt gtccccgaga     660

aagagagtta tttgtagatc tttcttgact actcgtagtt aacaggtctg aacctattcc     720

atccgaatta tctggagtat ggttgaaaac cttttctaaa gctgaattat ttgcaaatgt     780

ctggacagca tctgatatag agtgtggtag aacggaagtt actttttgaa ctagtgctct     840

ttgtggattt agtaatgagt ttccgttacg ggataataga tcgtttgtct gagaagtagg     900

agactgtgca tcattttctg ggttctcgtg atagctattg tcatatactc tgttgtcaaa     960

aactctatta tcgtaagttc tattatcatt atttatatga tagtgaatta caggttggga    1020

tgttttctca ttggctccgg gggttggtcc gccatctggc atatcttcgg gaggtgtagg    1080

gaggccatct tggataggat gcgaatcttt tatatcttct aaaatatcat taaggacttt    1140

tctcaacagc tcaggaaaca cggctactgt atttaagtct cttgacagtt tacctatacc    1200

aaatccttta ctatcaatag tatttttacg ttgattgcgc aaactatcaa aggcaagttt    1260

tgcctgtgcg tcaactaaat catctagttt gagagatgaa acagtttcat tacttaactt    1320

taattcagca agaatattat tcttgataaa tgatgatact tcagtattga ggagtttttg    1380

tcccaatgat aaagcttcac cagatgtgtg ttgttgtgct aatagtgcaa gtctgtgctc    1440

aatgttttgt cgtaattgat ggagtttctt attctttgcc gaccatggtc caaaggcaga    1500

atcttgggat aatttatctt ttataattag ttccgaatac tgtgcgacaa ttttttgaag    1560

tcccttttcg ttttcaaaat taagatcatt ttgatgtact aaggagtaca aaacatttga    1620

aaattcgaga atctcgcttt tggcatacgc attcattgag ttggaataaa tgcttcttaa    1680

cacatcttct ttccctgagg aagaaaataa aaaatcaaga catttctgat taaaagacgt    1740

tgcaaagttt tctttttcag acactgatgc tggtgttttt agagaagttt gcgaactatg    1800

gatatcggat attttgcttt ttaactcgct gtattctgtt gatgatggtg aagttgcctt    1860

atataagaat gttggcgctt gagtattatt tacattatgc at                       1902


<210> 47
<211> 20
<212> PRT
<213> Chlamydia trachomatis L2


<220> 
<223> TarP VBS1

<400> 47
Leu Phe Gln Ala Ala Thr Gln Thr Thr Gln Ala Leu Ser Ser Leu Ile 
1               5                   10                  15      
Asp Thr Val Gly 
            20  

<210> 48
<211> 24
<212> PRT
<213> Chlamydia trachomatis L2


<220> 
<223> TarP VBS2

<400> 48
Leu Phe Gln Ala Ala Ala Val Thr Gln Ala Leu Gly Asn Val Ala Gly 
1               5                   10                  15      
Lys Val Asn Leu Ala Ile Gln Gly 
            20                  

<210> 49
<211> 19
<212> PRT
<213> Chlamydophila caviae GPIC


<220> 
<223> TarP VBS1

<400> 49
Leu Leu Glu Ala Ala Arg Asn Thr Thr Thr Met Leu Ser Lys Thr Leu 
1               5                   10                  15      
Ser Lys Val 
            

<210> 50
<211> 19
<212> PRT
<213> Chlamydophila caviae GPIC


<220> 
<223> TarP VBS2

<400> 50
Ile Pro Gly Ala Ala Ala Asn Val Thr Ala Thr Leu Ser Ser Val Ala 
1               5                   10                  15      
Asn Lys Ile 
            

<210> 51
<211> 19
<212> PRT
<213> Chlamydophila caviae GPIC


<220> 
<223> TarP VBS3

<400> 51
Leu His Gly Ala Ala Lys Gly Val Ala Asp Ser Leu Ser Asn Leu Leu 
1               5                   10                  15      
Gln Ala Ala 
            

<210> 52
<211> 19
<212> PRT
<213> Chlamydophila abortus


<220> 
<223> TarP VBS1

<400> 52
Leu Ala Asp Ala Ala Arg Asn Val Thr Thr Gln Leu Ser Lys Thr Leu 
1               5                   10                  15      
Ser Lys Ala 
            

<210> 53
<211> 19
<212> PRT
<213> Chlamydophila abortus


<220> 
<223> TarP VBS2

<400> 53
Ile Pro Glu Ala Ala Gly Asn Val Ile Gln Ala Leu Ser Asn Val Ala 
1               5                   10                  15      
Lys Lys Ile 
            

<210> 54
<211> 19
<212> PRT
<213> Chlamydophila abortus


<220> 
<223> TarP VBS3

<400> 54
Leu His Gly Ala Ala Arg Asp Val Ala Ser Ser Leu Ser Asn Leu Leu 
1               5                   10                  15      
Glu Ala Ala 
            

<210> 55
<211> 19
<212> PRT
<213> Chlamydophila felis


<220> 
<223> TarP VBS1

<400> 55
Leu Phe Asp Ala Ala Lys Gln Thr Thr Ala Gln Leu Ser Lys Met Ile 
1               5                   10                  15      
Tyr Arg Ala 
            

<210> 56
<211> 19
<212> PRT
<213> Chlamydophila felis


<220> 
<223> TarP VBS2

<400> 56
Ile Pro Gln Ala Ala Ala Asn Val Thr Gln Thr Leu Ser Asn Val Thr 
1               5                   10                  15      
Gln Lys Leu 
            

<210> 57
<211> 19
<212> PRT
<213> Chlamydophila felis


<220> 
<223> TarP VBS3

<400> 57
Leu Tyr Ala Ala Ala Gly Asn Val Ala Asp Ser Leu Ser Asn Leu Leu 
1               5                   10                  15      
Gln Ala Ala 
            

<210> 58
<211> 19
<212> PRT
<213> Chlamydia muridarum


<220> 
<223> TarP VBS1

<400> 58
Leu Phe Ala Ala Ala Arg Ala Thr Thr Gln Ser Leu Ser Ser Leu Ile 
1               5                   10                  15      
Gly Thr Val 
            

<210> 59
<211> 19
<212> PRT
<213> Chlamydia muridarum


<220> 
<223> TarP VBS2

<400> 59
Leu Tyr Asp Ala Ala Lys Asn Val Thr Gln Ala Leu Thr Ser Val Thr 
1               5                   10                  15      
Asn Lys Val 
            

<210> 60
<211> 26
<212> PRT
<213> Homo sapiens


<220> 
<223> Talin-1 Helix 46 (H46)

<400> 60
Tyr Thr Lys Lys Glu Leu Ile Glu Cys Ala Arg Arg Val Ser Glu Lys 
1               5                   10                  15      
Val Ser His Val Leu Ala Ala Leu Gln Ala 
            20                  25      

<210> 61
<211> 9
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> N-ter domain

<400> 61
Gly Asp Pro Tyr Leu Thr Arg Glu Thr 
1               5                   

<210> 62
<211> 55
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> Interdomain VBS3-VBS2

<400> 62
Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys 
1               5                   10                  15      
Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys 
            20                  25                  30          
Ile Thr Asp Ala Asn Thr Ile Asn Tyr Val Thr Glu Thr Asn Ala Asp 
        35                  40                  45              
Thr Ile Asp Lys Asn His Ala 
    50                  55  

<210> 63
<211> 55
<212> PRT
<213> Shigella sonnei 53G


<220> 
<223> Interdomain VBS3-VBS2

<400> 63
Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys 
1               5                   10                  15      
Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys 
            20                  25                  30          
Val Thr Asp Ala Asn Thr Ile Asn Tyr Val Thr Glu Thr Asn Ala Asp 
        35                  40                  45              
Thr Ile Asp Lys Asn His Ala 
    50                  55  

<210> 64
<211> 55
<212> PRT
<213> Shigella dysenteriae


<220> 
<223> Interdomain VBS3-VBS2

<400> 64
Gly Thr Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys 
1               5                   10                  15      
Asp Ile Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys 
            20                  25                  30          
Ile Thr Asp Ala Asn Thr Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp 
        35                  40                  45              
Thr Ile Asp Lys Asn His Ala 
    50                  55  

<210> 65
<211> 27
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> Interdomain VBS2-VBS1

<400> 65
Asp Asp Thr Ser Ala Glu Leu Leu Thr Glu Asp Ile Ser Asn Leu Lys 
1               5                   10                  15      
Asn Asn Asn Asp Ile Thr Ala Glu Asn Asn Asn 
            20                  25          

<210> 66
<211> 22
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS1

<400> 66
Ile Tyr Lys Ala Ala Lys Asp Val Thr Thr Ser Leu Ser Lys Val Leu 
1               5                   10                  15      
Lys Asn Ile Asn Lys Asp 
            20          

<210> 67
<211> 21
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS2

<400> 67
Ile Tyr Glu Lys Ala Lys Glu Val Ser Ser Ala Leu Ser Lys Val Leu 
1               5                   10                  15      
Ser Lys Ile Asp Asp 
            20      

<210> 68
<211> 25
<212> PRT
<213> Shigella flexneri


<220> 
<223> IpaA VBS3

<400> 68
Thr Arg Glu Thr Ile Phe Glu Ala Ser Lys Lys Val Thr Asn Ser Leu 
1               5                   10                  15      
Ser Asn Leu Ile Ser Leu Ile Gly Thr 
            20                  25  

<210> 69
<211> 66
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS1

<400> 69
atatataaag cagcaaaaga tgtaaccact tccctatcaa aagtattaaa gaatatcaat     60

aaggat                                                                66


<210> 70
<211> 63
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS2

<400> 70
atctatgaaa aggcaaaaga agtatctagc gccctcagca aggtattgtc aaaaattgac     60

gat                                                                   63


<210> 71
<211> 75
<212> DNA
<213> Shigella flexneri


<220> 
<223> IpaA VBS3

<400> 71
acacgggaaa cgatatttga agcttcaaaa aaagtaacaa actccctaag taatcttata     60

tctctcattg gaact                                                      75


<210> 72
<211> 5
<212> PRT
<213> Shigella flexneri


<220> 
<223> N-ter domain

<400> 72
Gly Asp Thr Tyr Leu 
1               5   

<210> 73
<211> 53
<212> PRT
<213> Shigella flexneri


<220> 
<223> Interdomain VBS3-VBS2

<400> 73
Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
1               5                   10                  15      
Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Val Thr 
            20                  25                  30          
Asp Ala Asn Ile Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
        35                  40                  45              
Asp Lys Asn His Ala 
    50              

<210> 74
<211> 25
<212> PRT
<213> Shigella flexneri


<220> 
<223> Interdomain VBS2-VBS1

<400> 74
Thr Ser Ala Glu Leu Leu Thr Asp Asp Ile Ser Asp Leu Lys Asn Asn 
1               5                   10                  15      
Asn Asp Ile Thr Ala Glu Asn Asn Asn 
            20                  25  

<210> 75
<211> 5
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> N-ter domain

<400> 75
Gly Asp Pro Tyr Leu 
1               5   

<210> 76
<211> 53
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> Interdomain VBS3-VBS2

<400> 76
Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
1               5                   10                  15      
Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Ile Thr 
            20                  25                  30          
Asp Ala Asn Thr Ile Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
        35                  40                  45              
Asp Lys Asn His Ala 
    50              

<210> 77
<211> 53
<212> PRT
<213> Shigella sonnei 53G


<220> 
<223> Interdomain VBS3-VBS2

<400> 77
Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
1               5                   10                  15      
Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Val Thr 
            20                  25                  30          
Asp Ala Asn Thr Ile Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
        35                  40                  45              
Asp Lys Asn His Ala 
    50              

<210> 78
<211> 53
<212> PRT
<213> Shigella dysenteriae


<220> 
<223> Interdomain VBS3-VBS2

<400> 78
Lys Ser Gly Thr Gln Glu Arg Glu Leu Gln Glu Lys Ser Lys Asp Ile 
1               5                   10                  15      
Thr Lys Ser Thr Thr Glu His Arg Ile Asn Asn Lys Leu Lys Ile Thr 
            20                  25                  30          
Asp Ala Asn Thr Arg Asn Tyr Val Thr Glu Thr Asn Ala Asp Thr Ile 
        35                  40                  45              
Asp Lys Asn His Ala 
    50              

<210> 79
<211> 25
<212> PRT
<213> Shigella flexneri 5a


<220> 
<223> Interdomain VBS2-VBS1

<400> 79
Thr Ser Ala Glu Leu Leu Thr Glu Asp Ile Ser Asn Leu Lys Asn Asn 
1               5                   10                  15      
Asn Asp Ile Thr Ala Glu Asn Asn Asn 
            20                  25  

