                         SEQUENCE LISTING

<110>  The Board of Trustees of the Leland Stanford Junior 
       University
 
<120>  NUCLEASE-MEDIATED GENOME EDITING OF PRIMARY CELLS AND ENRICHMENT 
       THEREOF

<130>  1020125

<140>  PCT/US2016/053344
<141>  2016-09-23

<150>  US 62/232,713
<151>  2015-09-25

<150>  US 62/332,431
<151>  2016-05-05

<150>  US 62/357,832
<151>  2016-07-01

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  Target DNA sequence, HBB gene locus

<400>  1
ccgttactgc cctgtggggc aag                                               23


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  HBB on-target sgRNA target sequence

<400>  2
cttgcccaca gggcagtaac gg                                                22


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB off-target sgRNA target sequence

<400>  3
tcagcccaca gggcagtaac gg                                                22


<210>  4
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  rAAV6 HBB donor DNA sequence

<400>  4
gtcgagaagt ctgcagtcac tgctctatgg gggaag                                 36


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  Recombinant HBB sequence

<400>  5
gaggagaagt ctgccgttac tgccctgtgg ggcaag                                 36


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  Recombinant HBB sequence

<400>  6
gtcgagaagt ctgcagtcac tgctctstgg gggaaa                                 36


<210>  7
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Recombinant HBB sequence

<400>  7
gtcgagaagt ctgcagtcac tgccctgtgg ggcaag                                 36


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Recombinant HBB sequence

<400>  8
gaggagaagt ctgcagtcac tgctctatgg gggaaa                                 36


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Recombinant HBB sequence

<400>  9
gtcgagaagt ctgcagtcac tgctctatgg ggcaag                                 36


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Insert-3' homology arm for the HBB locus

<400>  10
acgtttcgcg cctgtggggc aagg                                              24


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic 3' homology arm-genomic locus for HBB

<400>  11
ctatgggacc cttgatgttt tctt                                              24


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Insert-3' homology arm for the CCR5 locus

<400>  12
tgggctacta gttgggctca ctat                                              24


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic 3' homology arm-genomic locus for CCR5

<400>  13
atctgtgggc ttgtgacacg gact                                              24


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Insert-3' homology arm for the IL2RG locus

<400>  14
acgtttaaac actagtgaag agca                                              24


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic 3' homology arm-genomic locus for IL2RG

<400>  15
gtcataagtc ggttgagggg agat                                              24


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB primer sequence

<400>  16
gaagatatgc ttagaaccga gg                                                22


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB primer sequence

<400>  17
ccacatgccc agtttctatt gg                                                22


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  18
gtaccagcac gccttcaaga cc                                                22


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 primer sequence

<400>  19
gcacagggtg gaacaagatg g                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 primer sequence

<400>  20
aagggggagg attgggaaga c                                                 21


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 primer sequence

<400>  21
tcaagaatca gcaattctct gaggc                                             25


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SFFV primer sequence

<400>  22
accgcagata tcctgtttgg                                                   20


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB primer sequence

<400>  23
gatcctgaga cttccacact gatgc                                             25


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  24
tcactagcaa cctcaaacag ac                                                22


<210>  25
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  25
atccacgttc accttgcc                                                     18


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic probe sequence

<400>  26
taacggcaga cttctccaca ggagtca                                           27


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG primer sequence

<400>  27
cagatatcca gagcctagcc tcatc                                             25


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG primer sequence

<400>  28
tcacacagca catatttgcc acaccctctg                                        30


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG primer sequence

<400>  29
tgcccacatg attgtaatgg ccagtgg                                           27


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  30
gggctttgac tttgggattt c                                                 21


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic probe sequence

<400>  31
ttcgaaagct tcggcgacct ca                                                22


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  32
cggcatggac gagctgtaca ag                                                22


<210>  33
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic probe sequence

<400>  33
tgactgcgga tttttcctca ggagtca                                           27


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  34
gtgtatccct ggacacaaag at                                                22


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 sgRNA target sequence

<400>  35
ggcagcatag tgagcccaga agg                                               23


<210>  36
<211>  2402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sickle cell disease nucleotide correction donor 
       sequence

<400>  36
aatttagtac aaggggaaaa agtacagggg gatgggagaa aggcgatcac gttgggaagc       60

tatagagaaa gaagagtaaa ttttagtaaa ggaggtttaa acaaacaaaa tataaagaga      120

aataggaact tgaatcaagg aaatgatttt aaaacgcagt attcttagtg gactagagga      180

aaaaaataat ctgagccaag tagaagacct tttcccctcc tacccctact ttctaagtca      240

cagaggcttt ttgttccccc agacactctt gcagattagt ccaggcagaa acagttagat      300

gtccccagtt aacctcctat ttgacaccac tgattacccc attgatagtc acactttggg      360

ttgtaagtga ctttttattt atttgtattt ttgactgcat taagaggtct ctagtttttt      420

atctcttgtt tcccaaaacc taataagtaa ctaatgcaca gagcacattg atttgtattt      480

attctatttt tagacataat ttattagcat gcatgagcaa attaagaaaa acaacaacaa      540

atgaatgcat atatatgtat atgtatgtgt gtatatatac acacatatat atatatattt      600

tttcttttct taccagaagg ttttaatcca aataaggaga agatatgctt agaaccgagg      660

tagagttttc atccattctg tcctgtaagt attttgcata ttctggagac gcaggaagag      720

atccatctac atatcccaaa gctgaattat ggtagacaaa actcttccac ttttagtgca      780

tcaacttctt atttgtgtaa taagaaaatt gggaaaacga tcttcaatat gcttaccaag      840

ctgtgattcc aaatattacg taaatacact tgcaaaggag gatgttttta gtagcaattt      900

gtactgatgg tatggggcca agagatatat cttagaggga gggctgaggg tttgaagtcc      960

aactcctaag ccagtgccag aagagccaag gacaggtacg gctgtcatca cttagacctc     1020

accctgtgga gccacaccct agggttggcc aatctactcc caggagcagg gagggcagga     1080

gccagggctg ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctgaca     1140

caactgtgtt cactagcaac ctcaaacaga caccatggtg cacctgactc ctgaggaaaa     1200

atccgcagtc actgccctgt ggggcaaggt gaacgtggat gaagttggtg gtgaggccct     1260

gggcaggttg gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcatg     1320

tggagacaga gaagactctt gggtttctga taggcactga ctctctctgc ctattggtct     1380

attttcccac ccttaggctg ctggtggtct acccttggac ccagaggttc tttgagtcct     1440

ttggggatct gtccactcct gatgctgtta tgggcaaccc taaggtgaag gctcatggca     1500

agaaagtgct cggtgccttt agtgatggcc tggctcacct ggacaacctc aagggcacct     1560

ttgccacact gagtgagctg cactgtgaca agctgcacgt ggatcctgag aacttcaggg     1620

tgagtctatg ggacgcttga tgttttcttt ccccttcttt tctatggtta agttcatgtc     1680

ataggaaggg gataagtaac agggtacagt ttagaatggg aaacagacga atgattgcat     1740

cagtgtggaa gtctcaggat cgttttagtt tcttttattt gctgttcata acaattgttt     1800

tcttttgttt aattcttgct ttcttttttt ttcttctccg caatttttac tattatactt     1860

aatgccttaa cattgtgtat aacaaaagga aatatctctg agatacatta agtaacttaa     1920

aaaaaaactt tacacagtct gcctagtaca ttactatttg gaatatatgt gtgcttattt     1980

gcatattcat aatctcccta ctttattttc ttttattttt aattgataca taatcattat     2040

acatatttat gggttaaagt gtaatgtttt aatatgtgta cacatattga ccaaatcagg     2100

gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact tttttgttta     2160

tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat acaatgtatc     2220

atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa ggcaatagca     2280

atatctctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga ggtttcatat     2340

tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt gggataaggc     2400

tg                                                                    2402


<210>  37
<211>  3720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sickle cell disease HBB cDNA-tNGFR enrichment donor 
       sequence

<400>  37
gtcctgtaag tattttgcat attctggaga cgcaggaaga gatccatcta catatcccaa       60

agctgaatta tggtagacaa aactcttcca cttttagtgc atcaacttct tatttgtgta      120

ataagaaaat tgggaaaacg atcttcaata tgcttaccaa gctgtgattc caaatattac      180

gtaaatacac ttgcaaagga ggatgttttt agtagcaatt tgtactgatg gtatggggcc      240

aagagatata tcttagaggg agggctgagg gtttgaagtc caactcctaa gccagtgcca      300

gaagagccaa ggacaggtac ggctgtcatc acttagacct caccctgtgg agccacaccc      360

tagggttggc caatctactc ccaggagcag ggagggcagg agccagggct gggcataaaa      420

gtcagggcag agccatctat tgcttacatt tgcttctgac acaactgtgt tcactagcaa      480

cctcaaacag acaccatggt gcacctgact cctgaagaaa aatccgctgt cacagccctc      540

tgggataagg tcaacgtcga tgccgtcggc ggcgaagctc tcggaagact cctcgtcgtg      600

tatccctgga cacaaagatt tttcgaaagc ttcggcgacc tcagcacccc cgacgccgtg      660

atgggaaatc ccaaagtcaa agcccacgga aaaaaggtcc tgggcgcttt cagcgacgga      720

ctcgcccatc tcgataatct gaaaggaaca ttcgctcagc tcagcgaact ccattgcgat      780

aaactccatg tcgaccccga aaattttaga ctgctcggaa atgtcctcgt gtgcgtcctc      840

gctcaccatt tcggaaagga gtttacaccc cctgtccaag ccgcttacca aaaggtcgtt      900

gccggcgtcg ccaacgctct cgctcataaa taccattgac tgtgccttct agttgccagc      960

catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg     1020

tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc     1080

tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg     1140

ctggggatgc ggtgggctct atggggctcc ggtgcccgtc agtgggcaga gcgcacatcg     1200

cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg     1260

tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt     1320

gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt     1380

gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt     1440

tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc     1500

gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg     1560

cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg     1620

caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga     1680

cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac     1740

actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca     1800

tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa     1860

gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg     1920

gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct     1980

gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc     2040

acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac     2100

cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt     2160

tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt     2220

aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct     2280

tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg     2340

tgacgccacc atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct     2400

gttgctgctt ctgggggtgt cccttggagg tgccaaggag gcatgcccca caggcctgta     2460

cacacacagc ggtgagtgct gcaaagcctg caacctgggc gagggtgtgg cccagccttg     2520

tggagccaac cagaccgtgt gtgagccctg cctggacagc gtgacgttct ccgacgtggt     2580

gagcgcgacc gagccgtgca agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc     2640

gccatgcgtg gaggccgacg acgccgtgtg ccgctgcgcc tacggctact accaggatga     2700

gacgactggg cgctgcgagg cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc     2760

ctgccaggac aagcagaaca ccgtgtgcga ggagtgcccc gacggcacgt attccgacga     2820

ggccaaccac gtggacccgt gcctgccctg caccgtgtgc gaggacaccg agcgccagct     2880

ccgcgagtgc acacgctggg ccgacgccga gtgcgaggag atccctggcc gttggattac     2940

acggtccaca cccccagagg gctcggacag cacagccccc agcacccagg agcctgaggc     3000

acctccagaa caagacctca tagccagcac ggtggcgggt gtggtgacca cagtgatggg     3060

cagctcccag cccgtggtga cccgaggcac caccgacaac ctcatccctg tctattgctc     3120

catcctggct gctgtggttg tgggtcttgt ggcctacata gccttcaaga ggtaaaactt     3180

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa     3240

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcctgccc     3300

tgtggggcaa ggtgaacgtg gatgaagttg gtggtgaggc cctgggcagg ttggtatcaa     3360

ggttacaaga caggtttaag gagaccaata gaaactgggc atgtggagac agagaagact     3420

cttgggtttc tgataggcac tgactctctc tgcctattgg tctattttcc cacccttagg     3480

ctgctggtgg tctacccttg gacccagagg ttctttgagt cctttgggga tctgtccact     3540

cctgatgctg ttatgggcaa ccctaaggtg aaggctcatg gcaagaaagt gctcggtgcc     3600

tttagtgatg gcctggctca cctggacaac ctcaagggca cctttgccac actgagtgag     3660

ctgcactgtg acaagctgca cgtggatcct gagaacttca gggtgagtct atgggacgct     3720


<210>  38
<211>  2469
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB SFFV-GFP donor sequence

<400>  38
gtcctgtaag tattttgcat attctggaga cgcaggaaga gatccatcta catatcccaa       60

agctgaatta tggtagacaa aactcttcca cttttagtgc atcaacttct tatttgtgta      120

ataagaaaat tgggaaaacg atcttcaata tgcttaccaa gctgtgattc caaatattac      180

gtaaatacac ttgcaaagga ggatgttttt agtagcaatt tgtactgatg gtatggggcc      240

aagagatata tcttagaggg agggctgagg gtttgaagtc caactcctaa gccagtgcca      300

gaagagccaa ggacaggtac ggctgtcatc acttagacct caccctgtgg agccacaccc      360

tagggttggc caatctactc ccaggagcag ggagggcagg agccagggct gggcataaaa      420

gtcagggcag agccatctat tgcttacatt tgcttctgac acaactgtgt tcactagcaa      480

cctcaaacag acaccatggt gcacctgact cctgaggaga agtctgccgt tactgcccat      540

taccctgtta tccctaccga taaaataaaa gattttattt agtctccaga aaaagggggg      600

aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc      660

atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata      720

gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca      780

agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag      840

atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag      900

gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt      960

tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc     1020

agtcctccga cagactgagt cgcccggggg ggtaccgagc tcttcgaagg atccatcgcc     1080

accatgcccg ccatgaagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc     1140

gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa caagatgaag     1200

agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc     1260

ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac     1320

aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg     1380

agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc     1440

ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg     1500

gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc     1560

ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc     1620

atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag     1680

ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc     1740

gccttcgcca gatctcgagt ctagctcgag ggcgcgcccg ctgatcagcc tcgacctgtg     1800

ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa     1860

ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt     1920

aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa     1980

gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacg     2040

tttcgcgcct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc ctgggcaggt     2100

tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca tgtggagaca     2160

gagaagactc ttgggtttct gataggcact gactctctct gcctattggt ctattttccc     2220

acccttaggc tgctggtggt ctacccttgg acccagaggt tctttgagtc ctttggggat     2280

ctgtccactc ctgatgctgt tatgggcaac cctaaggtga aggctcatgg caagaaagtg     2340

ctcggtgcct ttagtgatgg cctggctcac ctggacaacc tcaagggcac ctttgccaca     2400

ctgagtgagc tgcactgtga caagctgcac gtggatcctg agaacttcag ggtgagtcta     2460

tgggacgct                                                             2469


<210>  39
<211>  2295
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 SFFV-GFP donor sequence

<400>  39
aagagagtta attcaatgta gacatctatg taggcaatta aaaacctatt gatgtataaa       60

acagtttgca ttcatggagg gcaactaaat acattctagg actttataaa agatcacttt      120

ttatttatgc acagggtgga acaagatgga ttatcaagtg tcaagtccaa tctatgacat      180

caattattat acatcggagc cctgccaaaa aatcaatgtg aagcaaatcg cagcccgcct      240

cctgcctccg ctctactcac tggtgttcat ctttggtttt gtgggcaaca tgctggtcat      300

cctcatcctg ataaactgca aaaggctgaa gagcatgact gacatctacc tgctcaacct      360

ggccatctct gacctgtttt tccttcttac tgtccccttc tctagaccga taaaataaaa      420

gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa      480

gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa      540

gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg      600

gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc      660

ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag      720

acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa      780

ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa      840

aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggtt      900

cgaaggatcc atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc      960

catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg     1020

cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct     1080

gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg     1140

ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt     1200

ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa     1260

gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga     1320

cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat     1380

ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga     1440

cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt     1500

gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga     1560

gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat     1620

ggacgagctg tacaagtaac tcgagggcgc gccccgctga tcagcctcga ctgtgccttc     1680

tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc     1740

cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg     1800

tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa     1860

tagcaggcat gctggggatg cggtgggcta ctagttgggc tcactatgct gccgcccagt     1920

gggactttgg aaatacaatg tgtcaactct tgacagggct ctattttata ggcttcttct     1980

ctggaatctt cttcatcatc ctcctgacaa tcgataggta cctggctgtc gtccatgctg     2040

tgtttgcttt aaaagccagg acggtcacct ttggggtggt gacaagtgtg atcacttggg     2100

tggtggctgt gtttgcgtct ctcccaggaa tcatctttac cagatctcaa aaagaaggtc     2160

ttcattacac ctgcagctct cattttccat acagtcagta tcaattctgg aagaatttcc     2220

agacattaaa gatagtcatc ttggggctgg tcctgccgct gcttgtcatg gtcatctgct     2280

actcgggaat cctaa                                                      2295


<210>  40
<211>  3084
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG SFFV-GFP donor (variant 1)

<400>  40
tgggagaaac accacagaag cagagtgggt tatattctct gggtgagaga gggggagaaa       60

ttgaagctga ttctgaggtt tcaagtctgg gtgactgaga gggtgacgat accattgact      120

gaggtgggga aggcaggaag agaagcagag ttgggggaag atgggaagct tgaagctagt      180

attgttgttc ctccatttct agaatatttt tgtattataa gtcacacttc ctcgccagtc      240

tcaacaggga cccagctcag gcagcagcta agggtgggta ttctggtttg gattagatca      300

gaggaaagac agctgtatat gtgcccacag gagccaagac ggtattttcc atcctcccaa      360

aacagtagag ctttgacaga gatttaaggg tgaccaagtc aaggaagagg catggcatag      420

aacggtgatg tcgggggtgg gggttcagaa cttccattat agaaggtaat gatttagagg      480

agaaggtggt tgagaatggt gctagtggta gtgaacagat ccttcccagg atctaggtgg      540

gctgaggatt tttgagtctg tgacactatt gtatatccag ctttagtttc tgtttaccac      600

cttacagcag cacctaatct cctagaggac ttagcccgtg tcacacagca catatttgcc      660

acaccctctg taaagccctg gtttataagg ttctttccac cggaagctat gacagaggaa      720

acgtgtgggt ggggaggggt agtgggtgag ggacccaggt tcctgacaca gacagactac      780

acccagggaa tgaagagcaa gcgcctctag aattaccctg ttatccctac cgataaaata      840

aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg      900

caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga      960

gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct     1020

gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc     1080

cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt     1140

aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt     1200

gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta     1260

taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg     1320

gggatccatc gccaccatgc ccgccatgaa gatcgagtgc cgcatcaccg gcaccctgaa     1380

cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccgagcagg gccgcatgac     1440

caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt     1500

gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct     1560

gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg     1620

cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa     1680

ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag     1740

caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc     1800

ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca     1860

cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg     1920

ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt     1980

caagaccccc atcgccttcg ccagatctcg agtctagctc gagggcgcgc cccgctgatc     2040

agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc     2100

cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc     2160

gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg     2220

ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga     2280

ggcggaaaga acgtttaaac actagtgaag agcaagcgcc atgttgaagc catcattacc     2340

attcacatcc ctcttattcc tgcagctgcc cctgctggga gtggggctga acacgacaat     2400

tctgacgccc aatgggaatg aagacaccac agctggtggg aaatctggga ctggaggggg     2460

ctggtgagaa gggtggctgt gggaaggggc cgtacagaga tctggtgcct gccactggcc     2520

attacaatca tgtgggcaga attgaaaagt ggagtgggaa gggcaagggg gagggttccc     2580

tgcctcacgc tacttcttct ttctttcttg tttgtttgtt tctttctttc ttttgaggca     2640

gggtctcact atgttgccta ggctggtctc aaactcctgg cctctagtga tcctcctgcc     2700

tcagcctttc aaagcaccag gattacagac atgagccacc gtgcttggcc tcctccttct     2760

gaccatcatt tctctttccc tccctgcctt cattttctcc ccaatctaga tttcttcctg     2820

accactatgc ccactgactc cctcagtgtt tccactctgc ccctcccaga ggttcagtgt     2880

tttgtgttca atgtcgagta catgaattgc acttggaaca gcagctctga gccccagcct     2940

accaacctca ctctgcatta ttggtatgag aagggacgag ggggagggga tgaagaagag     3000

gtgggttgga tcagagacca agagagaggg tagcaagtct cccaggtacc ccactgtttt     3060

ctcctggggt aagtcataag tcgg                                            3084


<210>  41
<211>  2269
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG SFFV-GFP donor (variant 2)

<400>  41
gcatggcata gaacggtgat gtcgggggtg ggggttcaga acttccatta tagaaggtaa       60

tgatttagag gagaaggtgg ttgagaatgg tgctagtggt agtgaacaga tccttcccag      120

gatctaggtg ggctgaggat ttttgagtct gtgacactat tgtatatcca gctttagttt      180

ctgtttacca ccttacagca gcacctaatc tcctagagga cttagcccgt gtcacacagc      240

acatatttgc cacaccctct gtaaagccct ggtttataag gttctttcca ccggaagcta      300

tgacagagga aacgtgtggg tggggagggg tagtgggtga gggacccagg ttcctgacac      360

agacagacta cacccaggga atgaagagca agcgccatgt agcgccccga taaaataaaa      420

gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa      480

gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa      540

gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg      600

gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc      660

ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag      720

acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa      780

ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa      840

aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg      900

ggtaccgagc tcttcgaagg atccatcgcc accatgcccg ccatgaagat cgagtgccgc      960

atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc     1020

gagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc     1080

tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc     1140

tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag     1200

aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc     1260

gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc     1320

gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg     1380

ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg     1440

gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc     1500

cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg     1560

gagtaccagc acgccttcaa gacccccatc gccttcgcca gatctcgagt ctagttaatt     1620

aactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     1680

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     1740

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     1800

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggcct gcaggtgaag     1860

ccatcattac cattcacatc cctcttattc ctgcagctgc ccctgctggg agtggggctg     1920

aacacgacaa ttctgacgcc caatgggaat gaagacacca cagctggtgg gaaatctggg     1980

actggagggg gctggtgaga agggtggctg tgggaagggg ccgtacagag atctggtgcc     2040

tgccactggc cattacaatc atgtgggcag aattgaaaag tggagtggga agggcaaggg     2100

ggagggttcc ctgcctcacg ctacttcttc tttctttctt gtttgtttgt ttctttcttt     2160

cttttgaggc agggtctcac tatgttgcct aggctggtct caaactcctg gcctctagtg     2220

atcctcctgc ctcagccttt caaagcacca ggattacaga catgagcca                 2269


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB sgRNA target sequence

<400>  42
cttgccccac agggcagtaa cgg                                               23


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic CCR5 sgRNA target sequence

<400>  43
gcagcatagt gagcccagaa ggg                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic IL2RG sgRNA target sequence

<400>  44
tggtaatgat ggcttcaaca tgg                                               23


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB primer sequence

<400>  45
ggtgacaatt tctgccaatc agg                                               23


<210>  46
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB primer sequence

<400>  46
gaatggtagc tggattgtag ctgc                                              24


<210>  47
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB off-target sgRNA sequence

<400>  47
cttgccccac agggcagtaa cgg                                               23


<210>  48
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB variant

<400>  48
gtcgagaagt ctgcagtcac tgctctatgg gggaaa                                 36


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB off-target site

<400>  49
tcagccccac agggcagtaa ggg                                               23


<210>  50
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB allele that has undergone HR

<400>  50
gtcgagaagt ctgcagtcac tgctctatgg gggaaa                                 36


<210>  51
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic wild-type HBB allele

<400>  51
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta ctgccctgtg       60

gggcaagtga acgtggatga agttggtggt gaggccct                               98


<210>  52
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  52
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta ccctgtgggg       60

caagtgaacg tggatgaagt tggtggtgag gccct                                  95


<210>  53
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  53
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccctgt ggggcaagtg       60

aacgtggatg aagttggtgg tgaggccct                                         89


<210>  54
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  54
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta ccctgtgggg       60

caagtgaacg tggatgaagt tggtggtgag gccct                                  95


<210>  55
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  55
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtgc cctgtggggc       60

aagtgaacgt ggatgaagtt ggtggtgagg ccct                                   94


<210>  56
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  56
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgttg ccctgtgggg       60

caagtgaacg tggatgaagt tggtggtgag gccct                                  95


<210>  57
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  57
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgcccctg tggggcaagt       60

gaacgtggat gaagttggtg gtgaggccct                                        90


<210>  58
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  58
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta caagtgaacg       60

tggatgaagt tggtggtgag gccct                                             85


<210>  59
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  59
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta tgccctgtgg       60

ggcaagtgaa cgtggatgaa gttggtggtg aggccct                                97


<210>  60
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  60
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtac tgccctgtgg       60

ggcaagtgaa cgtggatgaa gttggtggtg aggccct                                97


<210>  61
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  61
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtgg ggcaagtgaa       60

cgtggatgaa gttggtggtg aggccct                                           87


<210>  62
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  62
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccctgt ggggcaagtg       60

aacgtggatg aagttggtgg tgaggccct                                         89


<210>  63
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  63
tcaaacagac accatggtgc acctgactcc tgaggagaag tcttgccctg tggggcaagt       60

gaacgtggat gaagttggtg gtgaggccct                                        90


<210>  64
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  64
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta gccctgtggg       60

gcaagtgaac gtggatgaag ttggtggtga ggccct                                 96


<210>  65
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  65
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgtta tgtggggcaa       60

gtgaacgtgg atgaagttgg tggtgaggcc ct                                     92


<210>  66
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  66
tcaacagaca catggtgcac ctgactcctg aggagaagtc aatgcctgtg gggcaaggtg       60

aacgtggatg aagttggtgg tgaggccct                                         89


<210>  67
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  67
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgccgttc tgccctgtgg       60

ggcaagtgaa cgtggatgaa gttggtggtg aggccct                                97


<210>  68
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  68
tcaaacagac accatggtgc acctgactcc tgaggagaag tctgctgccc tgtggggcaa       60

gtgaacgtgg atgaagttgg tggtgaggcc ct                                     92


<210>  69
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic HBB Variant

<400>  69
tcaacagaca ccatggtgca cctgactcct gaggagaact ctgccggctg ccctgtgggg       60

caaggtgaac gtggatgaag ttggtggtga ggcccct                                97


<210>  70
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  70
tatttgtgta ataagaaaat tgggaaaac                                         29


<210>  71
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  71
gttactgccc attaccctgt tatccctacc g                                      31


<210>  72
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  72
gtggagaagt ctgccgttac tgccctgtgg ggcaag                                 36


<210>  73
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  73
gaggaaaaat cccgcagtca ctgcccctgt ggggcaag                               38


<210>  74
<211>  455
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  74
catggtgcac ctgactcctg aagaaaaatc cgctgtcaca gccctctggg ataaggtcaa       60

cgtcgatgcc gtcggcggcg aagctctcgg aagactcctc ggtcgtgtat ccctggacac      120

aaagattttt cgaaagcttc ggcgacctca gcacccccga cgccgtgatg ggaaatccca      180

aagtcaaagc ccacggaaaa aaaggtcctg ggcgctttca gcgacggact cgcccatctc      240

gataatctga aaggaacatt cgctcagctc agcgaactcc attgcgataa actccatgtc      300

gacccccgaa aattttagac tgctcggaaa tgtcctcgtg tgcgtcctcg ctcaccattt      360

cggaaaggag tttacacccc ctgtccaagc cgcttaccaa aagggtcgtt gccggcgtcg      420

ccaacgctct cgctcataaa taccattgac tgtgc                                 455


