                         SEQUENCE LISTING

<110>  University of the Witwatersrand, Johannesburg
 
<120>  Process and Device for Removing Metal from a Liquid

<130>  P3277PC00

<150>  ZA 2015/02096
<151>  2015-09-26

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  870
<212>  DNA
<213>  Cupriavidus metallidurans CH34 - pbrD gene sequence

<400>  1
gccccaacgc cgcctcatcg atcgcgcgcg ccaaagctcg tgtcggaacc cattggcccc       60

cttgcgcaat gaatcgcgcg gacgcgtcaa cgacctacct acaggcgtag gcaccgtcgt      120

tgggttgctt gctctcatcc acggatgcgg cgaagacggg ggcaacgacc gactccgcga      180

gcgcaaattg ctgcttttcg aatgcccctg gatcgaggca accttcggca tcgaacgtga      240

gaatggaaaa cgtcgttcga acgacgcgca ctggctctct ggccaccagt tcgacgacca      300

catcggccat gcgtgcgcgt tgcccggcaa aagtggacaa gcagtggttg gccggctccc      360

gcaacatccg ctggaatttg gtggttggca gctgccagag agtatcgtca cgggcaatga      420

gaaactttcg gcaagaaaac cccatagctg cccccatgga ctttgagatt ccacaatgtc      480

tcgtcccgct cggacggttc tccccgcaca tcgtacaccg agagcatgac cgggacttgc      540

gctgtgacga aggtcgcttc ccggccagaa gcccccctgg ctccctcgca tgaaagccgc      600

cattccgtgc cgttttcgcc cccgatgtcg cgcgtcgcct gccaacgaaa tcatgcaagt      660

tttgggttgt agggcggcgc ctcgcgccag acgtcgttgc aaatcagcca aatacactgg      720

catcatgggt gttcggctac tgtctcttat gcggttgcga ccatgctcca cgaccagatc      780

gttgacatcc tcctgagtag ggcgtccact cccgaacaac tgagtgcgcc gagctgtcca      840

ggcgtgtatg cattctttct caattgccaa                                       870


<210>  2
<211>  289
<212>  PRT
<213>  Cupriavidus metallidurans CH34 - PbrD protein sequence

<400>  2

Met Ala Ile Glu Lys Glu Cys Ile His Ala Trp Thr Ala Arg Arg Thr 
1               5                   10                  15      


Gln Leu Phe Gly Ser Gly Arg Pro Thr Gln Glu Asp Val Asn Asp Leu 
            20                  25                  30          


Val Val Glu His Gly Arg Asn Arg Ile Arg Asp Ser Ser Arg Thr Pro 
        35                  40                  45              


Met Met Pro Val Tyr Leu Ala Asp Leu Gln Arg Arg Leu Ala Arg Gly 
    50                  55                  60                  


Ala Ala Leu Gln Pro Lys Thr Cys Met Ile Ser Leu Ala Gly Asp Ala 
65                  70                  75                  80  


Arg His Arg Gly Arg Lys Arg His Gly Met Ala Ala Phe Met Arg Gly 
                85                  90                  95      


Ser Gln Gly Gly Phe Trp Pro Gly Ser Asp Leu Arg His Ser Ala Ser 
            100                 105                 110         


Pro Gly His Ala Leu Gly Val Arg Cys Ala Gly Arg Thr Val Arg Ala 
        115                 120                 125             


Gly Arg Asp Ile Val Glu Ser Gln Ser Pro Trp Gly Gln Leu Trp Gly 
    130                 135                 140                 


Phe Leu Ala Glu Ser Phe Ser Leu Pro Val Thr Ile Leu Ser Gly Ser 
145                 150                 155                 160 


Cys Gln Pro Pro Asn Ser Ser Gly Cys Cys Gly Ser Arg Pro Thr Thr 
                165                 170                 175     


Ala Cys Pro Leu Leu Pro Gly Asn Ala His Ala Trp Pro Met Trp Ser 
            180                 185                 190         


Ser Asn Trp Trp Pro Glu Ser Gln Cys Ala Ser Phe Glu Arg Arg Phe 
        195                 200                 205             


Pro Phe Ser Arg Ser Met Pro Lys Val Ala Ser Ile Gln Gly His Ser 
    210                 215                 220                 


Lys Ser Ser Asn Leu Arg Ser Arg Ser Arg Ser Leu Pro Pro Ser Ser 
225                 230                 235                 240 


Pro His Pro Trp Met Arg Ala Ser Asn Pro Ser Leu Thr Arg Pro Arg 
                245                 250                 255     


Asp Ser Leu Arg Lys Gly Ala Asn Gly Phe Arg His Glu Leu Trp Arg 
            260                 265                 270         


Ala Arg Ser Met Arg Arg Arg Trp Gly Asn Asp Gly Ala Tyr Ala Cys 
        275                 280                 285             


Arg 
    


<210>  3
<211>  33
<212>  PRT
<213>  Cupriavidus metallidurans CH34 - PbrD hypothetical binding site


<220>
<221>  misc_feature
<222>  (2)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (19)..(32)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Cys Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Cys 
    


<210>  4
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence

<400>  4
ggtattgagg gtcgcttggc aattgag                                           27


<210>  5
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence

<400>  5
agaggagagt tagagcccta cctacagg                                          28


