                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  ASPARAGINASE

<130>  31324-WO-PCT

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  325
<212>  PRT
<213>  Thermoanaerobacter tengcongensis

<400>  1

Met Gly Lys Val Ala Val Ile Phe Thr Gly Gly Thr Ile Ala Met Lys 
1               5                   10                  15      


Gln Asp Pro Asn Leu Lys Gly Ala Val Pro Ser Leu Thr Gly Lys Asp 
            20                  25                  30          


Leu Leu Glu Ile Ile Pro His Arg Glu Asn Val Leu Glu Ile Glu Thr 
        35                  40                  45              


Ile Glu Phe Gly Asn Tyr Pro Ser Pro His Leu Thr Val Glu Leu Leu 
    50                  55                  60                  


Glu Glu Leu Arg Glu Val Val Glu Lys Thr Leu Lys Arg Asp Glu Ile 
65                  70                  75                  80  


Lys Gly Val Val Ile Thr His Gly Thr Asp Thr Leu Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Glu Leu Thr Thr Asn Phe Asp Lys Pro Ile Val Leu Thr 
            100                 105                 110         


Gly Ala Met Lys Asn Ser Ser Glu Ala Gly Tyr Asp Gly Pro Ser Asn 
        115                 120                 125             


Leu Ile Asp Ser Leu Thr Thr Ala Ser Ser Glu Lys Ser Lys Lys Arg 
    130                 135                 140                 


Gly Val Leu Val Val Leu Asn Gly Glu Ile His Ser Ala Lys Asp Val 
145                 150                 155                 160 


Thr Lys Thr His Thr Ser Ser Leu Asp Thr Phe Lys Ser Pro Glu Thr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Val Asp Ser Gly Asn Val Tyr Phe Tyr Arg Asn 
            180                 185                 190         


Val Glu Lys Arg Gln Tyr Ile Pro Thr Lys Lys Leu Glu Arg Arg Val 
        195                 200                 205             


Ser Leu Leu Lys Val Val Phe Gly Met Asp Asp Lys Leu Ile Arg Phe 
    210                 215                 220                 


Leu Val Glu Asn Gly Glu Arg Gly Ile Val Ile Glu Gly Thr Gly Arg 
225                 230                 235                 240 


Gly Asn Val Pro Pro Lys Val Ala Glu Gly Ile Lys Tyr Ala Ile Ser 
                245                 250                 255     


Lys Gly Thr Val Val Val Leu Val Ser Arg Cys Pro Met Gly Arg Val 
            260                 265                 270         


His Pro Ser Tyr Ala Tyr Glu Gly Gly Gly Lys His Leu Glu Ser Leu 
        275                 280                 285             


Gly Val Ile Phe Gly Gly Asn Leu Ser Gly Gln Lys Ala Arg Val Lys 
    290                 295                 300                 


Leu Met Ala Ala Leu Ala Tyr Thr Ser Asp Tyr Arg Lys Ile Arg Glu 
305                 310                 315                 320 


Ile Phe Glu Glu Glu 
                325 


<210>  2
<211>  997
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CpO sequence of putative Thermoanaerobacter tengcongensis 
       asparaginase for expression in E.coli

<400>  2
catatgggca aagtagcggt catttttact ggtggtacga ttgcgatgaa acaagacccg       60

aatctgaaag gtgcggttcc gagcttgacc ggcaaagatc tgctggagat tatcccacat      120

cgcgaaaatg ttctggaaat cgaaaccatt gagttcggta actatccgag cccgcacctg      180

acggttgagc tgctggaaga attgcgcgaa gttgtcgaga aaaccctgaa acgtgacgaa      240

atcaagggcg tggtgatcac ccatggtacg gacaccctgg aagaaaccgc gtacttttta      300

gaactgacca ccaatttcga taagccgatc gtgctgacgg gtgctatgaa gaacagctcc      360

gaggccggct acgatggtcc gtcaaatctg attgacagcc tgacgacggc atcgagcgag      420

aaaagcaaaa agcgtggcgt gctggttgtg ttgaatggtg agatccatag cgctaaagac      480

gtcaccaaga cccacacctc ttccctggac acctttaaga gcccggaaac cggccctctg      540

ggctatgttg atagcggtaa cgtctacttc taccgtaatg tcgagaagcg tcagtatatt      600

ccgactaaga aactggagcg tcgcgtcagc ctgttgaaag ttgtttttgg tatggatgac      660

aagttgattc gtttcctggt ggagaacggt gagcgtggta ttgtgatcga aggcacgggc      720

cgtggtaacg ttccgccgaa agtcgccgag ggtatcaaat atgcaatttc caagggtacc      780

gttgtggtgc tggtcagccg ctgcccgatg ggtcgtgtcc acccgagcta tgcgtacgag      840

ggtggcggca agcacctgga gtctctgggt gtgatctttg gcggcaacct gagcggtcag      900

aaagcgcgtg ttaagctgat ggcagccctg gcatacacga gcgattaccg taagatccgc      960

gagattttcg aagaagagta acctgcaggg gcgcgcc                               997

