                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  ASPARAGINASE

<130>  31322-WO-PCT

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  343
<212>  PRT
<213>  Fervidobacterium pennivorans

<400>  1

Met Ile Ser Gly Asn Trp Met Thr Lys Arg Lys Lys Lys Ile Ile Ile 
1               5                   10                  15      


Ile Ser Thr Gly Gly Thr Ile Ala Met Val Lys Lys Gly Arg Thr Val 
            20                  25                  30          


Val Pro Tyr Asp Lys Gly Asn Ala Leu Val Thr Asp Ile Pro Glu Leu 
        35                  40                  45              


Tyr Asp Ile Thr Asp Val Glu Leu Tyr Glu Phe Ser Asn Ile Pro Ser 
    50                  55                  60                  


Pro Tyr Met Thr Pro Lys Leu Met Phe Glu Leu Ala Arg Lys Ile Asp 
65                  70                  75                  80  


Glu Phe Leu Glu Glu Lys Gly Tyr Asp Gly Ala Val Val Thr His Gly 
                85                  90                  95      


Thr Asp Thr Leu Glu Glu Thr Ala Tyr Phe Leu Asp Leu Val Leu Lys 
            100                 105                 110         


Thr Glu Lys Pro Val Val Leu Thr Ala Ser Met Arg Asn Ile Ser Glu 
        115                 120                 125             


Leu Ser Thr Asp Gly Pro Arg Asn Val Leu Ser Ser Val Leu Val Ala 
    130                 135                 140                 


Ser Cys Asp Asp Ser Tyr Gly Met Gly Val Val Val Cys Leu Asn Asp 
145                 150                 155                 160 


Glu Ile His Ala Ala Arg Glu Val Thr Lys Thr Tyr Thr Ser Asn Val 
                165                 170                 175     


Ala Thr Phe Asp Ser Pro Gly Tyr Gly Pro Leu Gly Leu Val Asp Glu 
            180                 185                 190         


Asn Asn Val Ile Tyr Phe Arg Lys Ser Leu Thr Arg Glu Lys Ile Pro 
        195                 200                 205             


Thr Asp Arg Val Glu Glu Arg Val Ala Val Val Lys Thr Phe Thr Gly 
    210                 215                 220                 


Asp Asp Gly Thr Ile Leu Arg Thr Val Tyr Glu Met Gly Tyr Lys Gly 
225                 230                 235                 240 


Ile Val Leu Glu Gly Phe Gly Arg Gly Asn Val Pro Pro Glu Leu Ala 
                245                 250                 255     


Asp Val Val Glu Glu Ile Val Lys Lys Gly Ile Pro Val Val Ile Thr 
            260                 265                 270         


Ser Arg Cys Phe Lys Gly Arg Val Tyr Pro Ile Tyr Gly Tyr Thr Gly 
        275                 280                 285             


Gly Gly Ala Asp Leu Arg Arg Arg Gly Ala Ile Leu Ser Glu His Pro 
    290                 295                 300                 


Leu Ala Gln Lys Ala Arg Ile Lys Leu Met Val Val Leu Gly Ile Thr 
305                 310                 315                 320 


Asn Asp Ile Glu Glu Ile Arg Lys Tyr Phe Glu Lys His Ile Gly Gly 
                325                 330                 335     


Val Val Ser Glu Val Glu His 
            340             


<210>  2
<211>  1051
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CpO sequence of putative Fervidobacterium pennivorans 
       asparaginase for expression in E.coli

<400>  2
catatgatta gcggcaattg gatgaccaaa cgtaaaaaga aaatcattat catcagcacg       60

ggtggcacga tcgcaatggt caaaaagggt cgtaccgtgg tgccgtacga taagggtaac      120

gcgctggtca cggacatccc agagttgtat gatattactg acgttgagct gtatgaattc      180

agcaatattc cgagcccata catgaccccg aaactgatgt ttgagctggc tcgcaagatc      240

gatgagtttc tggaagaaaa gggttacgac ggtgccgttg tgacccatgg caccgacacg      300

ctggaagaaa ccgcgtactt cctggatctg gtgctgaaaa ccgagaaacc ggttgtcctg      360

accgcgagca tgcgtaatat cagcgagttg tccaccgatg gtccgcgtaa cgtgctgagc      420

tctgtgctgg ttgcgagctg cgacgatagc tacggtatgg gtgtggtcgt ttgtctgaac      480

gacgaaatcc acgcagcacg cgaagtgacc aagacctata ccagcaatgt tgcgacgttc      540

gactctccgg gttatggtcc gctgggcttg gttgatgaga acaatgtcat ttactttcgc      600

aaatccctga cccgtgagaa aattccgacg gaccgtgtcg aggaacgtgt tgcagtcgta      660

aaaacgttca ccggtgacga cggcaccatt ctgcgcacgg tctatgaaat gggttacaag      720

ggtattgtgc tggagggttt tggtcgcggt aacgttccgc ctgagctggc tgatgtcgtt      780

gaagaaattg tgaaaaaggg catcccggtc gttatcacta gccgttgctt taagggtcgt      840

gtctatccga tctacggtta tacgggtggc ggcgcggacc ttcgccgtcg tggcgccatc      900

ctgtcggagc acccgctggc gcagaaagcc cgtattaagt tgatggttgt gctgggcatt      960

actaacgata ttgaagagat ccgcaagtac ttcgagaagc acattggcgg cgttgtgagc     1020

gaagtggagc attaacctgc aggggcgcgc c                                    1051




