
                                SEQUENCE LISTING

<110> Massachusetts Institute of Technology
      Chen, Jianzhu
      Drake, Adam C.
      Kaur, Mandeep
      Phennicie, Ryan T.

<120> A Humanized Mouse Model of De Novo Human
  Acute Myeloid Leukemia with a Matching Human Immune System
  

<130> 0050.2281001

<140> PCT/US2016/052003   
<141> 2016-09-15  

<150> 62/219,105          
<151> 2015-09-15  

<160> 22

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1449
<212> DNA
<213> Homo sapiens

<400> 1
agaaaggagt ggggttgaaa agcgcttgcg caggacggct acggtacggg ggtgggaggg 60
cttcggagca cgcgcgcgga ggcgggactt gggaagcgct cgcgagatct tcagggtcta 120
tatataagcg cggggagcct gcgtcctttc cctggtgtga ttccgtcctg cgcggttgtt 180
ctctggagca gcgttctttt atctccgtcc gccttctctc ctacctaagt gcgtgccgcc 240
acccgatgga agattcgatg gacatggaca tgagccccct gaggccccag aactatcttt 300
tcggttgtga actaaaggcc gacaaagatt atcactttaa ggtggataat gatgaaaatg 360
agcaccagtt atctttaaga acggtcagtt taggggctgg tgcaaaggat gagttgcaca 420
ttgttgaagc agaggcaatg aattacgaag gcagtccaat taaagtaaca ctggcaactt 480
tgaaaatgtc tgtacagcca acggtttccc ttgggggctt tgaaataaca ccaccagtgg 540
tcttaaggtt gaagtgtggt tcagggccag tgcatattag tggacagcac ttagtagctg 600
tggaggaaga tgcagagtca gaagatgaag aggaggagga tgtgaaactc ttaagtatat 660
ctggaaagcg gtctgcccct ggaggtggta gcaaggttcc acagaaaaaa gtaaaacttg 720
ctgctgatga agatgatgac gatgatgatg aagaggatga tgatgaagat gatgatgatg 780
atgattttga tgatgaggaa gctgaagaaa aagcgccagt gaagaaatct atacgagata 840
ctccagccaa aaatgcacaa aagtcaaatc agaatggaaa agactcaaaa ccatcatcaa 900
caccaagatc aaaaggacaa gaatccttca agaaacagga aaaaactcct aaaacaccaa 960
aaggacctag ttctgtagaa gacattaaag caaaaatgca agcaagtata gaaaaaggtg 1020
gttctcttcc caaagtggaa gccaaattca tcaattatgt gaagaattgc ttccggatga 1080
ctgaccaaga ggctattcaa gatctctggc agtggaggaa gtctctttaa gaaaatagtt 1140
taaacaattt gttaaaaaat tttccgtctt atttcatttc tgtaacagtt gatatctggc 1200
tgtccttttt ataatgcaga gtgagaactt tccctaccgt gtttgataaa tgttgtccag 1260
gttctattgc caagaatgtg ttgtccaaaa tgcctgttta gtttttaaag atggaactcc 1320
accctttgct tggttttaag tatgtatgga atgttatgat aggacatagt agtagcggtg 1380
gtcagacatg gaaatggtgg ggagacaaaa atatacatgt gaaataaaac tcagtatttt 1440
aataaagta                                                         1449

<210> 2
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 2
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctgtctgg cagtggagga agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 3
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 3
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctgcatgg cagtggagga agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 4
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 4
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctgcgtgg cagtggagga agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 5
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 5
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctgcctgg cagtggagga agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 6
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 6
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctggcagt ctcttgccca agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 7
<211> 1092
<212> DNA
<213> Homo sapiens

<400> 7
ggttgttctc tggagcagcg ttcttttatc tccgtccgcc ttctctccta cctaagtgcg 60
tgccgccacc cgatggaaga ttcgatggac atggacatga gccccctgag gccccagaac 120
tatcttttcg gttgtgaact aaaggccgac aaagattatc actttaaggt ggataatgat 180
gaaaatgagc accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgag 240
ttgcacattg ttgaagcaga ggcaatgaat tacgaaggca gtccaattaa agtaacactg 300
gcaactttga aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca 360
ccagtggtct taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta 420
gtagctgtgg aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta 480
agtatatctg gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta 540
aaacttgctg ctgatgaaga tgatgacgat gatgatgaag aggatgatga tgaagatgat 600
gatgatgatg attttgatga tgaggaagct gaagaaaaag cgccagtgaa gaaatctata 660
cgagatactc cagccaaaaa tgcacaaaag tcaaatcaga atggaaaaga ctcaaaacca 720
tcatcaacac caagatcaaa aggacaagaa tccttcaaga aacaggaaaa aactcctaaa 780
acaccaaaag gacctagttc tgtagaagac attaaagcaa aaatgcaagc aagtatagaa 840
aaaggtggtt ctcttcccaa agtggaagcc aaattcatca attatgtgaa gaattgcttc 900
cggatgactg accaagaggc tattcaagat ctctggcagt ccctggagaa agtctcttta 960
agaaaatagt ttaaacaatt tgttaaaaaa ttttccgtct tatttcattt ctgtaacagt 1020
tgatatctgg ctgtcctttt tataatgcag agtgagaact ttccctaccg tgtttgataa 1080
atgttgtcca gg                                                     1092

<210> 8
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Tubulin forward primer

<400> 8
ccagatcttt agaccagaca ac                                          22

<210> 9
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Tubulin reverse primer

<400> 9
caggacagaa tcaaccagct c                                           21

<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Human CD34 forward primer

<400> 10
gagacaacct tgaagcctag                                             20

<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Human CD34 reverse primer

<400> 11
ctgagtcaat ttcacttctc tg                                          22

<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA9 forward primer

<400> 12
cttgtggttc tcctccagtt g                                           21

<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA9 reverse primer

<400> 13
catgaagcca gttggctgct g                                           21

<210> 14
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA5 forward primer

<400> 14
gcaagctgca cataagtc                                               18

<210> 15
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA5 reverse primer

<400> 15
ccagatttta atttgtctct cgg                                         23

<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA6 forward primer

<400> 16
gtttaccctt ggatgcagc                                              19

<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> HOXA6 reverse primer

<400> 17
gtagcggttg aagtggaact c                                           21

<210> 18
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Myc forward primer

<400> 18
ctccagcttg tacctgcagg atctgag                                     27

<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Myc reverse primer

<400> 19
gagcctgcct cttttccaca g                                           21

<210> 20
<211> 7573
<212> DNA
<213> Artificial Sequence

<220> 
<223> pL3-2A-EGFP plasmid

<400> 20
gggccagcgg ccgcgaattc ggatccgcca ccatggtgag caagggcgag gagctgttca 60
ccggggtggt gcccatcctg gtcgagctgg acggagatgt aaacggccac aagttcagcg 120
tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 180
ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 240
agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 300
ccgaaggcta cgtccaggag agaacaatct tcttcaaaga tgatggaaac tacaagaccc 360
gcgccgaggt caagttcgag ggcgacaccc tggtcaaccg catcgagctg aagggcatcg 420
acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 480
acgtctatat catggccgac aagcagaaga acggcatcaa agtgaacttc aagatccgcc 540
acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 600
gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 660
aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 720
tcactctcgg catggacgag ctgtacaccg gtgtgaaaca gactttgaat tttgaccttc 780
tcaagttggc gggagacgtg gagtccaacc ccgggccgac gacgacgacg tctagatagg 840
taagtaggtg agggcccacg acgacggttt aaaccacatg tttaagggtt ccggttccac 900
taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 960
agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 1020
atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 1080
tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 1140
tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 1200
ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 1260
cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 1320
gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 1380
acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 1440
ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 1500
ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg 1560
agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 1620
agaggaggag gaggtgggtt ttccagtcac acctcaggta ccaagcatgg ggtaaagtac 1680
tgttctcatc acatcatatc aaggttatat accatcaata ttgccacaga tgttacttag 1740
ccttttaata tttctctaat ttagtgtata tgcaatgata gttctctgat ttctgagatt 1800
gagtttctca tgtgtaatga ttatttagag tttctctttc atctgttcaa atttttgtct 1860
agttttattt tttactgatt tgtaagactt ctttttataa tctgcatatt acaattctct 1920
ttactggggt gttgcaaata ttttctgtca ttctatggcc tgacttttct taatggtttt 1980
ttaattttaa aaataagtct taatattcat gcaatctaat taacaatctt ttctttgtgg 2040
ttaggacttt gagtcataag aaatttttct ctacactgaa gtcatgatgg catgcttcta 2100
tattattttc taaaagattt aaagttttgc cttctccatt tagacttata attcactgga 2160
atttttttgt gtgtatggta tgacatatgg gttccctttt attttttaca tataaatata 2220
tttccctgtt tttctaaaaa agaaaaagat catcattttc ccattgtaaa atgccatatt 2280
tttttcatag gtcacttaca tatatcaatg ggtctgtttc tgagctctac tctattttat 2340
cagcctcact gtctatcccc acacatctca tgctttgctc taaatcttga tatttagtgg 2400
aacattcttt cccattttgt tctacaagaa tatttttgtt attgtctttt gggcttctat 2460
atacatttta gaatgaggtt ggcaaggtac ctttaagacc aatgacttac aaggcagctg 2520
tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccagc 2580
tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 2640
gctaactagg gaacccactg cttaagcctc ataaagctcg actgtgcctt ctagttgcca 2700
gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2760
tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2820
tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 2880
tgctggggat gcggtccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg 2940
gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 3000
cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 3060
ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg 3120
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 3180
cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 3240
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 3300
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 3360
tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 3420
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 3480
gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 3540
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 3600
ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 3660
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3720
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3780
aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3840
atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3900
tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3960
tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 4020
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 4080
gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 4140
tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 4200
ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 4260
gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 4320
aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 4380
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 4440
tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 4500
ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 4560
aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 4620
ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 4680
ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4740
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4800
tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4860
ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 4920
ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 4980
agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag 5040
ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt 5100
ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtg 5160
tgcatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 5220
tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 5280
gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 5340
agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 5400
agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 5460
tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 5520
aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt 5580
cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac 5640
aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct 5700
tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt 5760
gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct ctggttagac 5820
cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 5880
agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 5940
agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg 6000
acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag 6060
cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 6120
gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 6180
cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata 6240
tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat 6300
cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag 6360
aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga 6420
taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca 6480
ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa 6540
ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 6600
caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 6660
gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 6720
ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 6780
tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 6840
aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 6900
ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 6960
tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta 7020
cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca 7080
agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg 7140
gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt 7200
ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca 7260
gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg 7320
agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat 7380
tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt 7440
acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 7500
aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag 7560
tttggttagt acc                                                    7573

<210> 21
<211> 9819
<212> DNA
<213> Artificial Sequence

<220> 
<223> pLB2U6(EF1alpha)GFP

<400> 21
tctagagatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg 60
agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata 120
gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta 180
catgataggc ttggatttct ataagagata caaatactaa attattattt taaaaaacag 240
cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatcc 300
cttggagaaa agccttgttt gctacaactt ctacatgact tcaagagagt catgtagtag 360
ttgtagcttt tttggtaacc ctcgagagct ataagtaaag catctctgtg agttttattt 420
gttgcctgta gggattgttt aattctgcat tggaagtagc caaatttgaa ggtgctgtca 480
ttcgaactgt cagtggagcg gccgcagatc tcgaccaatt ctcatgtttg acagcttatc 540
atcgataagc tttggagcta agccagcaat ggtagaggga agattctgca cgtcccttcc 600
aggcggcctc cccgtcacca ccccccccaa cccgccccga ccggagctga gagtaattca 660
tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa 720
cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg 780
aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc 840
cacagtcccc gagaagttgg ggggaggggt cggcaattga accggccggt gcctagagaa 900
agtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 960
gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 1020
ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 1080
gttatggccc ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt 1140
gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc 1200
ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc 1260
tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt 1320
tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat 1380
ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag 1440
cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag 1500
tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc 1560
tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc 1620
ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag 1680
tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg 1740
gagtaccggg cgccgtccag gcacctcgat tagttctcga tcgagctttt ggagtacgtc 1800
gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga 1860
gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga 1920
gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt 1980
tcaggtgtcg tgaaaactct aggatgctag cgctaccggt cgccaccatg gtgagcaagg 2040
gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 2100
gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 2160
tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 2220
tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 2280
tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 2340
gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 2400
agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 2460
actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 2520
acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 2580
agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 2640
agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 2700
tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaggaa ttcggtttaa 2760
accacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa 2820
tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 2880
ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 2940
ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 3000
gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 3060
ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 3120
tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 3180
gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 3240
ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 3300
tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 3360
ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 3420
acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca 3480
atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac 3540
ctcaggtacc aagcatgggg taaagtactg ttctcatcac atcatatcaa ggttatatac 3600
catcaatatt gccacagatg ttacttagcc ttttaatatt tctctaattt agtgtatatg 3660
caatgatagt tctctgattt ctgagattga gtttctcatg tgtaatgatt atttagagtt 3720
tctctttcat ctgttcaaat ttttgtctag ttttattttt tactgatttg taagacttct 3780
ttttataatc tgcatattac aattctcttt actggggtgt tgcaaatatt ttctgtcatt 3840
ctatggcctg acttttctta atggtttttt aattttaaaa ataagtctta atattcatgc 3900
aatctaatta acaatctttt ctttgtggtt aggactttga gtcataagaa atttttctct 3960
acactgaagt catgatggca tgcttctata ttattttcta aaagatttaa agttttgcct 4020
tctccattta gacttataat tcactggaat ttttttgtgt gtatggtatg acatatgggt 4080
tcccttttat tttttacata taaatatatt tccctgtttt tctaaaaaag aaaaagatca 4140
tcattttccc attgtaaaat gccatatttt tttcataggt cacttacata tatcaatggg 4200
tctgtttctg agctctactc tattttatca gcctcactgt ctatccccac acatctcatg 4260
ctttgctcta aatcttgata tttagtggaa cattctttcc cattttgttc tacaagaata 4320
tttttgttat tgtcttttgg gcttctatat acattttaga atgaggttgg caaggtacct 4380
ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 4440
ggactggaag ggctaattca ctcccagctg ctttttgcct gtactgggtc tctctggtta 4500
gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcat 4560
aaagctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 4620
ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 4680
cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 4740
gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtccggact gtactgggtc 4800
tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 4860
taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 4920
ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat 4980
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 5040
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 5100
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 5160
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 5220
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 5280
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 5340
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 5400
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 5460
ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 5520
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 5580
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 5640
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 5700
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 5760
aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 5820
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 5880
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 5940
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 6000
cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 6060
tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 6120
cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 6180
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 6240
cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 6300
ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 6360
gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 6420
taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 6480
gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 6540
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 6600
aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 6660
cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 6720
atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 6780
gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca 6840
atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc 6900
gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc 6960
atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat 7020
acgcgttgac attgattatt gactagtcat gttctttcct gcgttatccc ctgattctgt 7080
ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 7140
gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 7200
cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 7260
cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 7320
ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 7380
aaacagctat gaccatgatt acgccaagcg cgcaattaac cctagcttaa tgtagtctta 7440
tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg 7500
agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt 7560
aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag 7620
agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga 7680
tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 7740
tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 7800
ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt 7860
gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg 7920
cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg 7980
ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg 8040
atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt 8100
atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga 8160
aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact 8220
tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa 8280
agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc 8340
acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg 8400
agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc 8460
aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc 8520
cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta 8580
caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt 8640
gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga 8700
atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct 8760
ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg 8820
gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca 8880
agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa 8940
ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg 9000
tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt 9060
gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc 9120
cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag 9180
agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg 9240
cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag 9300
tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa 9360
acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg 9420
gttagtaccg ggcccggtgc tttgctctga gccagcccac cagtttggaa tgactccttt 9480
ttatgacttg aattttcaag tataaagtct agtgctaaat ttaatttgaa caactgtata 9540
gtttttgctg gttgggggaa ggaaaaaaaa tggtggcagt gtttttttca gaattagaag 9600
tgaaatgaaa acttgttgtg tgtgaggatt tctaatgaca tgtggtggtt gcatactgag 9660
tgaagccggt gagcattctg ccatgtcacc ccctcgtgct cagtaatgta ctttacagaa 9720
atcctaaact caaaagattg atataaacca tgcttcttgt gtatatccgg tctcttctct 9780
gggtagtctc actcagcctg catttctgcc agggcccgc                        9819

<210> 22
<211> 8923
<212> DNA
<213> Artificial Sequence

<220> 
<223> pL3-PGK-eGFP-2A-NPM1-MutA

<400> 22
gggccagcgg ccgcgcaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga 60
gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc 120
acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg 180
ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc 240
aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct 300
gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct 360
ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg 420
ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc 480
acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgggatc cgccaccatg 540
gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacgga 600
gatgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 660
aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 720
gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 780
cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagagaac aatcttcttc 840
aaagatgatg gaaactacaa gacccgcgcc gaggtcaagt tcgagggcga caccctggtc 900
aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 960
ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 1020
atcaaagtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 1080
cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 1140
ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 1200
ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caccggtgtg 1260
aaacagactt tgaattttga ccttctcaag ttggcgggag acgtggagtc caaccccggg 1320
ccgatggaag attcgatgga catggacatg agccccctga ggccccagaa ctatcttttc 1380
ggttgtgaac taaaggccga caaagattat cactttaagg tggataatga tgaaaatgag 1440
caccagttat ctttaagaac ggtcagttta ggggctggtg caaaggatga gttgcacatt 1500
gttgaagcag aggcaatgaa ttacgaaggc agtccaatta aagtaacact ggcaactttg 1560
aaaatgtctg tacagccaac ggtttccctt gggggctttg aaataacacc accagtggtc 1620
ttaaggttga agtgtggttc agggccagtg catattagtg gacagcactt agtagctgtg 1680
gaggaagatg cagagtcaga agatgaagag gaggaggatg tgaaactctt aagtatatct 1740
ggaaagcggt ctgcccctgg aggtggtagc aaggttccac agaaaaaagt aaaacttgct 1800
gctgatgaag atgatgacga tgatgatgaa gaggatgatg atgaagatga tgatgatgat 1860
gattttgatg atgaggaagc tgaagaaaaa gcgccagtga agaaatctat acgagatact 1920
ccagccaaaa atgcacaaaa gtcaaatcag aatggaaaag actcaaaacc atcatcaaca 1980
ccaagatcaa aaggacaaga atccttcaag aaacaggaaa aaactcctaa aacaccaaaa 2040
ggacctagtt ctgtagaaga cattaaagca aaaatgcaag caagtataga aaaaggtggt 2100
tctcttccca aagtggaagc caaattcatc aattatgtga agaattgctt ccggatgact 2160
gaccaagagg ctattcaaga tctctgtctg gcagtggagg aagtctcttt acgcaagtga 2220
ggtaagtagg tgagggccca cgacgacggt ttaaaccaca tgtttaaggg ttccggttcc 2280
actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg 2340
aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 2400
taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 2460
aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2520
tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 2580
tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 2640
gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 2700
cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 2760
ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2820
tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2880
ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat 2940
ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 3000
caagaggagg aggaggtggg ttttccagtc acacctcagg taccaagcat ggggtaaagt 3060
actgttctca tcacatcata tcaaggttat ataccatcaa tattgccaca gatgttactt 3120
agccttttaa tatttctcta atttagtgta tatgcaatga tagttctctg atttctgaga 3180
ttgagtttct catgtgtaat gattatttag agtttctctt tcatctgttc aaatttttgt 3240
ctagttttat tttttactga tttgtaagac ttctttttat aatctgcata ttacaattct 3300
ctttactggg gtgttgcaaa tattttctgt cattctatgg cctgactttt cttaatggtt 3360
ttttaatttt aaaaataagt cttaatattc atgcaatcta attaacaatc ttttctttgt 3420
ggttaggact ttgagtcata agaaattttt ctctacactg aagtcatgat ggcatgcttc 3480
tatattattt tctaaaagat ttaaagtttt gccttctcca tttagactta taattcactg 3540
gaattttttt gtgtgtatgg tatgacatat gggttccctt ttatttttta catataaata 3600
tatttccctg tttttctaaa aaagaaaaag atcatcattt tcccattgta aaatgccata 3660
tttttttcat aggtcactta catatatcaa tgggtctgtt tctgagctct actctatttt 3720
atcagcctca ctgtctatcc ccacacatct catgctttgc tctaaatctt gatatttagt 3780
ggaacattct ttcccatttt gttctacaag aatatttttg ttattgtctt ttgggcttct 3840
atatacattt tagaatgagg ttggcaaggt acctttaaga ccaatgactt acaaggcagc 3900
tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 3960
gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 4020
tggctaacta gggaacccac tgcttaagcc tcataaagct cgactgtgcc ttctagttgc 4080
cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 4140
actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 4200
attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 4260
catgctgggg atgcggtccg gactgtactg ggtctctctg gttagaccag atctgagcct 4320
gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 4380
tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 4440
ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca 4500
ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4560
atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4620
aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 4680
gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 4740
ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 4800
ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 4860
acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 4920
gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 4980
ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5040
ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 5100
gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 5160
ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 5220
agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 5280
ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 5340
gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 5400
catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 5460
cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 5520
cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 5580
gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 5640
tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 5700
gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 5760
tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 5820
gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 5880
gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 5940
taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6000
tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 6060
ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 6120
taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 6180
tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 6240
aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 6300
atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag 6360
ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta 6420
agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg 6480
ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag 6540
tcatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 6600
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 6660
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 6720
gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 6780
gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 6840
gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 6900
agcgcgcaat taaccctagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca 6960
tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat 7020
tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat 7080
ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc 7140
gatacaataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7200
ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7260
gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7320
aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc 7380
tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac 7440
tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga 7500
gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag 7560
ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat 7620
tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 7680
tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 7740
ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga 7800
tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt 7860
cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt 7920
agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag 7980
agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag 8040
cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat 8100
agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact 8160
cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa 8220
ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt 8280
gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg 8340
gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga 8400
atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag 8460
tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat 8520
agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt 8580
taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga 8640
caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt 8700
agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca 8760
attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 8820
tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc 8880
gggtttatta cagggacagc agagatccag tttggttagt acc                   8923