               SEQUENCE LISTING

<110> LabGenius Ltd

<120> Compositions And Methods For Polynucleotide Assembly

<130> P40304WO

<160> 40

<170> BiSSAP 1.3.6

<210> 1
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence of SSP in Figure 3

<400> 1
ccctgactga cgatgagata agggctatga ttagaaag                             38


<210> 2
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence A of Example 2

<400> 2
atgtctccta ttctgggata ttggaagatc aaaggccttg tgcagcccac ccgtcttctt     60

ctggagtatc tcgaagagaa atatgaggaa cacctatacg agcgcgatga aggtgataaa    120

tggcggaaca agaagtttga gctcgggcta                                     150


<210> 3
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence B of Example 2

<400> 3
atgtcgccaa ttctaggtta ttggaagatc aaaggtctgg tacaacctac tcgtctgctt     60

cttgaatacc tgtaagaaaa gtacgaggaa catctttatg aacgcgatga aggggacaag    120

tggcgaaata agaagtttga actcggtctt                                     150


<210> 4
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence C of Example 2

<400> 4
atgtcgccaa tcctggggta ttggaagata aaaggccttg tccagcccac gcgactacta     60

ctcgaatacc tggaggagaa atatgaagaa cacctttacg agcgtgatga aggtgacaag    120

tggcgtaaca agaaatttga gcttgggcta                                     150


<210> 5
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence D of Example 2

<400> 5
atgtctccaa tactgggata ttggaaaata aaagggcttg ttcaaccaac ccgcctactt     60

ctggaatatc tggaagagaa gtatgaagag catctttatg aacgggatga gggtgacaaa    120

tggcgtaata agaagtttga acttggtctt                                     150


<210> 6
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence E of Example 2

<400> 6
atgtcaccaa tactcgggta ctggaaaatt aaaggcctcg tacagcctac ccgactgctt     60

ctagagtacc ttgaagaaaa gtatgaagag catctttatg agcgtgatga gggggacaaa    120

tggcgaaata aaaagtttga gcttgggctg                                     150


<210> 7
<211> 149
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence F of Example 2

<400> 7
atgtctcctt acttggatac tggaagatta agggactcgt tcaacctacg cgtcttctgc     60

tcgagtatct cgaggagaaa tacgaagaac atctctatga acgggacgag ggcgataaat    120

ggcgcaacaa gaagtttgaa ctggggctg                                      149


<210> 8
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence G of Example 2

<400> 8
atgtccccga tcctcggcta ctggaaaatt aaggggctcg ttcagcctac ccgtctgcta     60

ctcgaatacc tggaagaaaa atatgaggaa catctatatg aacgcgacga aggggacaag    120

tggcgaaata agaagttcga actagggctg                                     150


<210> 9
<211> 150
<212> DNA
<213> Artificial Sequence


<220> 
<223> Insert Sequence H of Example 2

<400> 9
atgtctccta ttctcgggta ttggaagatc aaagggctgg tccagcctac tcggctactt     60

ctggaatatc ttgaggaaaa atatgaggaa cacctgtatg aacgtgacga aggagataag    120

tggcgtaaca agaaattcga gctgggccta                                     150


<210> 10
<211> 50
<212> PRT
<213> Artificial Sequence


<220> 
<223> Target Protein Sequence of Example 2

<400> 10
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              
Gly Leu 
    50  

<210> 11
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein expressed by colony G in Example 2

<400> 11
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu 
            20                  

<210> 12
<211> 49
<212> PRT
<213> Artificial Sequence


<220> 
<223> Proetin sequence expressed by colony F in Example 2

<400> 12
Met Ser Pro Tyr Leu Asp Thr Gly Arg Leu Arg Asp Ser Phe Asn Leu 
1               5                   10                  15      
Arg Val Phe Cys Ser Ser Ile Ser Arg Arg Asn Thr Lys Asn Ile Ser 
            20                  25                  30          
Met Asn Gly Thr Arg Ala Ile Asn Gly Ala Thr Arg Ser Leu Asn Trp 
        35                  40                  45              
Gly 
    

<210> 13
<211> 2000
<212> DNA
<213> Mytilus californianus


<400> 13
atggagggaa tcaaattaaa tctgtgcctc ctgtgcatat tttcttgtga cgtctttgct      60

ctttcaaatg gtttcataca caacgcatat ggctcagctt atgcaggtgc aagcgctggg     120

gcttacaagc cattacccgg ttcatacgga tcaaagcatg taccagtata taaacctatg     180

aataagattc caacatcata tatatccaag aaaagttatc cggcacccta taaaccgaaa     240

ggctatcatc ctacgaatag ttatcagcca acatatggat caaagacaaa ctatccgcca     300

atatataagc cagttgcaaa gaagctatca tcatacaaag ctattaagac aacgtatctg     360

gtctataaag caaagacaag ttatccacca gtttataaac ataagataac taatcctcca     420

acatataaac ctaagattac ttatccccca acatataaac caaagccaag ttatcctcca     480

acatataaac caaagccaag ttatcctcca acatataaag caaagaaaac ttatccttca     540

acatataaac caaagccaag ttatcctcca acatataaac ctaagataac ttatcctcca     600

acatataaac caaagccaag ttatccgcct tcatataagg caaagaaatc atatccttca     660

acatataaac caaagccaag ttatcctcca acatataaag caaagaaaac ttatccttca     720

acatataaac caaagccaag ttatcctcca acatataaac ctaagataac ttatcctcca     780

acatataaac caaagccaag ttatccgcct tcatataagg caaagaaatc atatccttca     840

acatataaac caaagccaag ttatcctcct acatataaac ctaagataac ttatcctcca     900

acatataaac caaagccaag ttatcctcca acatataaag caaagaaaac ttatcctcca     960

acatataaac ctaagataac ttatcctcca acatataaac caaagccaag ttatcccact    1020

tcatataaat ctaagaaaac ttatcctcca acatataaac ctaaaataac ttatccacca    1080

acatataaac caaagccaag ttatccacca tcatataaac ctaagattac ttatcctcca    1140

acttataaac ctaagaaaag ttatcctcca gcatacaaat ctaaggcaag ttatccccct    1200

tcttatcaac ccaagaaaac ttatctgcca tcatataaac ctaagaaaac ttatcctcca    1260

acatataaac gtaagataag ttatccacca acgtataaaa caaagccaag ttatccatca    1320

tcttataaac gtaaaacaag ttatccatca acatataaac gtaagactag ttatcctcca    1380

acatataaac ctaagataag ttatccttca acttataaaa caaagccaag ttatccacca    1440

acgtataaag caaagaaaac ttatcctcca acatataaac ctaagataac ttatcctcca    1500

acatataaac caaagccaag ttatcccact tcatataaat ctaagaaaac ttatcctcca    1560

acatataaac ctaaaataac ttatccacca acatataaac caaagccaag ttatccacca    1620

tcatataaac ctaagattac ttatcctcca acttataaac ctaagaaaag ttatcctcca    1680

gcatacaaat ctaaggcaag ttatccccct tcttatcaac ccaagaaaac ttatctgcca    1740

tcatataaac ctaagaaaac ttatcctcca acatataaac gtaagataag ttatccacca    1800

acgtataaaa caaagccaag ttatccagca tcttataaac gtaaaacaag ttatccatca    1860

acatataaac gtaagacaag ttatcctcca acatataaac ctaagataag ttatccttca    1920

acttataaaa caaagccaag ttatccacca acgtataaac caaaaccaag ttatgcatca    1980

tcatataaac ctaagatacg                                                2000


<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 1 in Example 3

<400> 14
atgaugttgg aatcttattc atagg                                          25


<210> 15
<211> 199
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 1 in Example 3

<400> 15
atggagggaa tcaaattaaa tctgtgcctc ctgtgcatat tttcttgtga cgtctttgct     60

ctttcaaatg gtttcataca caacgcatat ggctcagctt atgcaggtgc aagcgctggg    120

gcttacaagc cattacccgg ttcatacgga tcaaagcatg taccagtata taaacctatg    180

aataagattc caacatcat                                                 199


<210> 16
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 2 in Example 3

<400> 16
aaacuggtgg ataacttgtc tttgc                                          25


<210> 17
<211> 200
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 2 in Example 3

<400> 17
atcauatata tccaagaaaa gttatccggc accctataaa ccgaaaggct atcatcctac     60

gaatagttat cagccaacat atggatcaaa gacaaactat ccgccaatat ataagccagt    120

tgcaaagaag ctatcatcat acaaagctat taagacaacg tatctggtct ataaagcaaa    180

gacaagttat ccaccagttt                                                200


<210> 18
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 3 in Example 3

<400> 18
atctuaggtt tatatgttgg aggataac                                       28


<210> 19
<211> 198
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 3 in Example 3

<400> 19
agttuataaa cataagataa ctaatcctcc aacatataaa cctaagatta cttatccccc     60

aacatataaa ccaaagccaa gttatcctcc aacatataaa ccaaagccaa gttatcctcc    120

aacatataaa gcaaagaaaa cttatccttc aacatataaa ccaaagccaa gttatcctcc    180

aacatataaa cctaagat                                                  198


<210> 20
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 4 in Example 3

<400> 20
aggauaagtt atcttaggtt tatatgttg                                      29


<210> 21
<211> 195
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 4 in Example 3

<400> 21
aagauaactt atcctccaac atataaacca aagccaagtt atccgccttc atataaggca     60

aagaaatcat atccttcaac atataaacca aagccaagtt atcctccaac atataaagca    120

aagaaaactt atccttcaac atataaacca aagccaagtt atcctccaac atataaacct    180

aagataactt atcct                                                     195


<210> 22
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 5 in Example 3

<400> 22
aggtutatat gttggaggat aagttttc                                       28


<210> 23
<211> 200
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 5 in Example 3

<400> 23
atccuccaac atataaacca aagccaagtt atccgccttc atataaggca aagaaatcat     60

atccttcaac atataaacca aagccaagtt atcctcctac atataaacct aagataactt    120

atcctccaac atataaacca aagccaagtt atcctccaac atataaagca aagaaaactt    180

atcctccaac atataaacct                                                200


<210> 24
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 6 in Example 3

<400> 24
aggataacut ttcttaggtt tataagttg                                      29


<210> 25
<211> 200
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 6 in Example 3

<400> 25
aaccuaagat aacttatcct ccaacatata aaccaaagcc aagttatccc acttcatata     60

aatctaagaa aacttatcct ccaacatata aacctaaaat aacttatcca ccaacatata    120

aaccaaagcc aagttatcca ccatcatata aacctaagat tacttatcct ccaacttata    180

aacctaagaa aagttatcct                                                200


<210> 26
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 7 in Example 3

<400> 26
atatgutgat ggataacttg ttttacg                                        27


<210> 27
<211> 198
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 7 in Example 3

<400> 27
agttatccuc cagcatacaa atctaaggca agttatcccc cttcttatca acccaagaaa     60

acttatctgc catcatataa acctaagaaa acttatcctc caacatataa acgtaagata    120

agttatccac caacgtataa aacaaagcca agttatccat catcttataa acgtaaaaca    180

agttatccat caacatat                                                  198


<210> 28
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 8 in Example 3

<400> 28
agatutatat gaagtgggat aacttgg                                        27


<210> 29
<211> 192
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 8 in Example 3

<400> 29
acatauaaac gtaagactag ttatcctcca acatataaac ctaagataag ttatccttca     60

acttataaaa caaagccaag ttatccacca acgtataaag caaagaaaac ttatcctcca    120

acatataaac ctaagataac ttatcctcca acatataaac caaagccaag ttatcccact    180

tcatataaat ct                                                        192


<210> 30
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 9 in Example 3

<400> 30
agataagutt tcttgggttg ataagaag                                       28


<210> 31
<211> 199
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 9 in Example 3

<400> 31
aatcuaagaa aacttatcct ccaacatata aacctaaaat aacttatcca ccaacatata     60

aaccaaagcc aagttatcca ccatcatata aacctaagat tacttatcct ccaacttata    120

aacctaagaa aagttatcct ccagcataca aatctaaggc aagttatccc ccttcttatc    180

aacccaagaa aacttatct                                                 199


<210> 32
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 10 in Example 3

<400> 32
ataagtugaa ggataactta tcttagg                                        27


<210> 33
<211> 198
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 10 in Example 3

<400> 33
acttatcugc catcatataa acctaagaaa acttatcctc caacatataa acgtaagata     60

agttatccac caacgtataa aacaaagcca agttatccag catcttataa acgtaaaaca    120

agttatccat caacatataa acgtaagaca agttatcctc caacatataa acctaagata    180

agttatcctt caacttat                                                  198


<210> 34
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer for Fragment 11 in Example 3

<400> 34
atcttaggut tatatgatga tgcataac                                       28


<210> 35
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fragment 11 in Example 3

<400> 35
aacttauaaa acaaagccaa gttatccacc aacgtataaa ccaaaaccaa gttatgcatc     60

atcatataaa cctaagat                                                   78


<210> 36
<211> 12
<212> DNA
<213> Artificial Sequence


<220> 
<223> Degenerate Sequence 1 Example 4

<400> 36
nnbnnbnnbn nb                                                        12


<210> 37
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Degenerate Sequence 2 Example 4

<400> 37
nnbnnbnnbn nbnnbnnbnn bnnbnnbnnb                                     30


<210> 38
<211> 152
<212> DNA
<213> Artificial Sequence


<220> 
<223> SSP design Example 5

<400> 38
cagcauttga cggctagctc agtcctaggt acagtgctag ctaatacgac tcactatagg     60

gagatactag agaccgtcga gtacctttac agctagctca gtcctaggta ttatgctagc    120

uactcacgtt agtgagtagc tagcauaata cc                                  152


<210> 39
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward vector linearisation primer Example 5

<400> 39
atgctagcug agaaatcaaa ttaaggaggt aag                                  33


<210> 40
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse vector linearisation primer Example 5

<400> 40
atgctgucca gaaatcatcc ttagcg                                         26


