               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Improved vitamin production

<130> CH 01239/15 

<160> 32

<170> BiSSAP 1.3.6

<210> 1
<211> 1284
<212> DNA
<213> Bacillus subtilis subsp. subtilis str. 168


<400> 1
atgaaagacg tatctatttc ctctttggaa aatatgaaat tgaaagagct ttatgaactt      60

gcaagacatt ataaaatctc ctattacagc aaactgacaa aaaaagaact cattttcgcc     120

attctgaaag cgaatgcaga acaggaagat ctgctgttta tggaaggcgt tctcgagatc     180

atccagtctg aaggtttcgg attcctgaga ccgatcaact actctccaag ctcagaagac     240

atttacatct cagcttcaca aatccgccgt ttcgatttgc ggaacggaga caaagtatct     300

ggcaaggttc gcccgccaaa agaaaatgag cgttactatg gacttttgca cgttgaagca     360

gtaaatgggg atgatcccga atctgcaaaa gagcgtgtgc atttcccggc tcttacgcca     420

ctttatccgg atcgtcaaat ggtgcttgaa acaaagccga acttcttgtc tacaagaatt     480

atggacatga tggcgccggt tggatttggg cagcgcggat tgattgttgc gccgccgaaa     540

gccggaaaaa cgatgttgct gaaggaaatt gccaacagca ttacagcgaa ccagcctgaa     600

gcagagctga tcgtgctttt aattgacgaa agacctgagg aagtaaccga tatcgagcgc     660

tctgtagctg gggatgtcgt cagctcaacg tttgatgaag tgccggaaaa ccatatcaaa     720

gtggccgagc ttgtgcttga acgtgcgatg cgtctcgtgg aacacaaaaa agacgtcatt     780

atcctgatgg acagcatcac acgtcttgcc cgcgcctaca acttagtgat tccgccaagt     840

ggaagaacgc tttccggggg gattgaccca gcggcgttcc accgtccgaa acgcttcttt     900

ggggctgcga gaaatatcga agagggcggc agcttaacca tccttgctac ggctctggtc     960

gatacaggtt cacgtatgga tgatgtcatt tatgaagaat tcaagggaac aggcaacatg    1020

gagctccatc ttgaccgctc tcttgccgag cgccgcatct tccctgccat cgatatccgc    1080

cgttcaggaa cgcgcaaaga agagctgctt gtgcctaaag agcatcttga tcgtttatgg    1140

tctatccgca aaacgatgtc tgattcacct gatttcgcag aaaagttcat gagaaaaatg    1200

aaaaaaacca aaacaaacca ggaatttttc gatattctca atcaagaatg gaaacaggca    1260

aatctatcat ctgcaagaag gtaa                                           1284


<210> 2
<211> 427
<212> PRT
<213> Bacillus subtilis subsp. subtilis str. 168


<400> 2
Met Lys Asp Val Ser Ile Ser Ser Leu Glu Asn Met Lys Leu Lys Glu 
1               5                   10                  15      
Leu Tyr Glu Leu Ala Arg His Tyr Lys Ile Ser Tyr Tyr Ser Lys Leu 
            20                  25                  30          
Thr Lys Lys Glu Leu Ile Phe Ala Ile Leu Lys Ala Asn Ala Glu Gln 
        35                  40                  45              
Glu Asp Leu Leu Phe Met Glu Gly Val Leu Glu Ile Ile Gln Ser Glu 
    50                  55                  60                  
Gly Phe Gly Phe Leu Arg Pro Ile Asn Tyr Ser Pro Ser Ser Glu Asp 
65                  70                  75                  80  
Ile Tyr Ile Ser Ala Ser Gln Ile Arg Arg Phe Asp Leu Arg Asn Gly 
                85                  90                  95      
Asp Lys Val Ser Gly Lys Val Arg Pro Pro Lys Glu Asn Glu Arg Tyr 
            100                 105                 110         
Tyr Gly Leu Leu His Val Glu Ala Val Asn Gly Asp Asp Pro Glu Ser 
        115                 120                 125             
Ala Lys Glu Arg Val His Phe Pro Ala Leu Thr Pro Leu Tyr Pro Asp 
    130                 135                 140                 
Arg Gln Met Val Leu Glu Thr Lys Pro Asn Phe Leu Ser Thr Arg Ile 
145                 150                 155                 160 
Met Asp Met Met Ala Pro Val Gly Phe Gly Gln Arg Gly Leu Ile Val 
                165                 170                 175     
Ala Pro Pro Lys Ala Gly Lys Thr Met Leu Leu Lys Glu Ile Ala Asn 
            180                 185                 190         
Ser Ile Thr Ala Asn Gln Pro Glu Ala Glu Leu Ile Val Leu Leu Ile 
        195                 200                 205             
Asp Glu Arg Pro Glu Glu Val Thr Asp Ile Glu Arg Ser Val Ala Gly 
    210                 215                 220                 
Asp Val Val Ser Ser Thr Phe Asp Glu Val Pro Glu Asn His Ile Lys 
225                 230                 235                 240 
Val Ala Glu Leu Val Leu Glu Arg Ala Met Arg Leu Val Glu His Lys 
                245                 250                 255     
Lys Asp Val Ile Ile Leu Met Asp Ser Ile Thr Arg Leu Ala Arg Ala 
            260                 265                 270         
Tyr Asn Leu Val Ile Pro Pro Ser Gly Arg Thr Leu Ser Gly Gly Ile 
        275                 280                 285             
Asp Pro Ala Ala Phe His Arg Pro Lys Arg Phe Phe Gly Ala Ala Arg 
    290                 295                 300                 
Asn Ile Glu Glu Gly Gly Ser Leu Thr Ile Leu Ala Thr Ala Leu Val 
305                 310                 315                 320 
Asp Thr Gly Ser Arg Met Asp Asp Val Ile Tyr Glu Glu Phe Lys Gly 
                325                 330                 335     
Thr Gly Asn Met Glu Leu His Leu Asp Arg Ser Leu Ala Glu Arg Arg 
            340                 345                 350         
Ile Phe Pro Ala Ile Asp Ile Arg Arg Ser Gly Thr Arg Lys Glu Glu 
        355                 360                 365             
Leu Leu Val Pro Lys Glu His Leu Asp Arg Leu Trp Ser Ile Arg Lys 
    370                 375                 380                 
Thr Met Ser Asp Ser Pro Asp Phe Ala Glu Lys Phe Met Arg Lys Met 
385                 390                 395                 400 
Lys Lys Thr Lys Thr Asn Gln Glu Phe Phe Asp Ile Leu Asn Gln Glu 
                405                 410                 415     
Trp Lys Gln Ala Asn Leu Ser Ser Ala Arg Arg 
            420                 425         

<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 3
cggatatgga tacatatcgg ttc                                            23


<210> 4
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 4
cgactaaagc agaaagagga agag                                           24


<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 5
ttcaactaac ggggcaggtt a                                              21


<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 6
ggttatcacc tgtgaaatag g                                              21


<210> 7
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 7
cagaggatca aaccggagaa acgg                                           24


<210> 8
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 8
gctcttggac ccgggatcct tatttccgca aattgctg                             38


<210> 9
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 9
cagcaatttg cggaaataag gatcccgggt ccaagagc                             38


<210> 10
<211> 52
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 10
tagaataaaa tttgcgtgcg ttgcaagcct tggaagctgt cagtagtata cc             52


<210> 11
<211> 52
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 11
ggtatactac tgacagcttc caaggcttgc aacgcacgca aattttattc ta             52


<210> 12
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 12
gtttcaacgg taagcgttct tccg                                           24


<210> 13
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 13
gcccacaggt gtatatg                                                   17


<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 14
gctcagttaa ttctttgatg cc                                             22


<210> 15
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 15
agagaatgaa gagactgcag agtg                                           24


<210> 16
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 16
ttctgcctcg taatctcccg aag                                            23


<210> 17
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 17
ctgaaagctt agttatccgt gc                                             22


<210> 18
<211> 47
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 18
gatctcgacc tgcagcccaa gccaccacgc ttttcatagt caatatc                   47


<210> 19
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 19
cttggggctg caggtcgaga tc                                             22


<210> 20
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 20
gttcaaaatg gtatgcgttt tgacac                                         26


<210> 21
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 21
gtgtcaaaac gcataccatt ttgaacggca aatctatcat ctgcaagaag g              51


<210> 22
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 22
ctgatcagct cttcagattt cc                                             22


<210> 23
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 23
ctcaggtgga atcagattgg c                                              21


<210> 24
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 24
gattcggtct gtccttcg                                                  18


<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 25
acatattccc gttatgcatc g                                              21


<210> 26
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 26
actggcacgg ttgttgcgtc c                                              21


<210> 27
<211> 43
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 27
gataataagg gtaactattg ccgagtcgct ccagttgcaa acg                       43


<210> 28
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 28
cggcaatagt tacccttatt atc                                            23


<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 29
ttataaaagc cagtcattag gcc                                            23


<210> 30
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 30
gtttttatat tacagctcca gatcgctgga cggacgaaga aattg                     45


<210> 31
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 31
catcctctac aacataaacg g                                              21


<210> 32
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 32
gatctggagc tgtaatataa aaac                                           24


