                         SEQUENCE LISTING

<110>  Biontech RNA Pharmaceuticals GmbH
 
<120>  Method for reducing immunogenicity of RNA

<130>  674-161 PCT

<150>  PCT/EP2015/069760
<151>  2015-08-28

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  192
<212>  PRT
<213>  Mus musculus

<400>  1

Met Gly Val Pro Glu Arg Pro Thr Leu Leu Leu Leu Leu Ser Leu Leu 
1               5                   10                  15      


Leu Ile Pro Leu Gly Leu Pro Val Leu Cys Ala Pro Pro Arg Leu Ile 
            20                  25                  30          


Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu Ala 
        35                  40                  45              


Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu Asn 
    50                  55                  60                  


Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg Met 
65                  70                  75                  80  


Glu Val Glu Glu Gln Ala Ile Glu Val Trp Gln Gly Leu Ser Leu Leu 
                85                  90                  95      


Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Leu Ala Asn Ser Ser Gln 
            100                 105                 110         


Pro Pro Glu Thr Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly Leu 
        115                 120                 125             


Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu Leu 
    130                 135                 140                 


Met Ser Pro Pro Asp Thr Thr Pro Pro Ala Pro Leu Arg Thr Leu Thr 
145                 150                 155                 160 


Val Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ala Asn Phe Leu Arg 
                165                 170                 175     


Gly Lys Leu Lys Leu Tyr Thr Gly Glu Val Cys Arg Arg Gly Asp Arg 
            180                 185                 190         


<210>  2
<211>  579
<212>  DNA
<213>  Mus musculus

<400>  2
atgggggtgc ccgaacgtcc caccctgctg cttttactct ccttgctact gattcctctg       60

ggcctcccag tcctctgtgc tcccccacgc ctcatctgcg acagtcgagt tctggagagg      120

tacatcttag aggccaagga ggcagaaaat gtcacgatgg gttgtgcaga aggtcccaga      180

ctgagtgaaa atattacagt cccagatacc aaagtcaact tctatgcttg gaaaagaatg      240

gaggtggaag aacaggccat agaagtttgg caaggcctgt ccctgctctc agaagccatc      300

ctgcaggccc aggccctgct agccaattcc tcccagccac cagagaccct tcagcttcat      360

atagacaaag ccatcagtgg tctacgtagc ctcacttcac tgcttcgggt actgggagct      420

cagaaggaat tgatgtcgcc tccagatacc accccacctg ctccactccg aacactcaca      480

gtggatactt tctgcaagct cttccgggtc tacgccaact tcctccgggg gaaactgaag      540

ctgtacacgg gagaggtctg caggagaggg gacaggtga                             579


<210>  3
<211>  579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC-rich sequence

<400>  3
atgggcgtcc ccgaaaggcc taccctgctg ctgctcctgt ctctgctcct gatccccctg       60

ggactgcccg tgctgtgcgc ccctcccagg ctgatctgcg acagcagggt gctggaaaga      120

tacatcctgg aagccaaaga ggccgagaac gtcacaatgg gctgcgccga gggccccaga      180

ctgagcgaga acatcaccgt gcccgacacc aaggtcaact tctacgcctg gaagaggatg      240

gaagtggagg aacaggccat cgaggtctgg cagggactgt ctctgctgtc cgaggccatc      300

ctgcaggccc aggctctgct ggccaattct agccagcccc ccgagacact gcagctgcac      360

atcgacaagg ccatcagcgg cctgagaagc ctgaccagcc tgctgagggt gctgggagcc      420

cagaaagagc tgatgagccc ccctgacacc accccccctg cccccctgag gaccctgacc      480

gtggacacct tctgcaagct gttcagggtg tacgccaact tcctgagggg caagctgaag      540

ctgtacaccg gcgaggtctg cagacggggc gacagatga                             579


<210>  4
<211>  579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A-rich sequence

<400>  4
atgggagtgc cagaaagacc aaccctgctg ctgctgctca gcctgctact gatcccactg       60

ggactcccag tcctctgcgc accaccaaga ctcatctgcg acagcagagt gctggaaaga      120

tacatcctag aagcaaaaga agcagaaaac gtcacgatgg gatgcgcaga aggaccaaga      180

ctgagcgaaa acatcacagt cccagacacc aaagtcaact tctacgcatg gaaaagaatg      240

gaagtggaag aacaggcaat agaagtgtgg caaggactga gcctgctcag cgaagcaatc      300

ctgcaggcac aggcactgct agcaaacagc agccagccac cagaaaccct gcagctgcac      360

atagacaaag caatcagcgg actaagaagc ctcaccagcc tgctgagagt actgggagca      420

cagaaagaac tgatgagccc accagacacc accccaccag caccactcag aacactcaca      480

gtggacactt tctgcaaact cttcagagtc tacgcaaact tcctcagagg aaaactgaaa      540

ctgtacacgg gagaagtctg cagaagaggg gacagatga                             579


<210>  5
<211>  579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Super optimized sequence

<400>  5
atgggagttc ctgaaagacc aactctgttg ctcttgctgt ctttgctgct gattcctctg       60

ggtcttccgg tgctttgcgc acctcccagg cttatctgcg atagcagggt gcttgagagg      120

tacatcctgg aagctaaaga agccgaaaac gtgaccatgg gctgcgccga gggccctagg      180

ctcagtgaaa acattactgt tcccgatacg aaagtcaatt tctacgcctg gaagcggatg      240

gaagtggagg aacaggccat agaggtgtgg caaggtctgt ctctcctgag cgaggcaatc      300

cttcaagccc aggctctgct ggccaattca agccagccac ccgagaccct ccagctgcac      360

attgacaagg ctatcagcgg tctgagatcc ctgacgtccc tgttgcgagt cctgggcgct      420

cagaaggagc tgatgagtcc acccgatacc acacctccag caccgctccg cacactcact      480

gtggacacct tttgtaaact gttcagagtc tacgccaact ttctgcgagg caagctgaag      540

ctctatacag gagaggtgtg taggagagga gaccggtga                             579


<210>  6
<211>  579
<212>  DNA
<213>  GC-maximized sequence

<400>  6
atgggcgtgc ccgagcggcc gaccctgctc ctgctgctca gcctgctgct catccccctg       60

gggctgcccg tcctctgcgc ccccccgcgc ctgatctgcg actcccgggt gctggagcgc      120

tacatcctcg aggccaagga ggcggagaac gtgaccatgg gctgcgccga ggggccccgg      180

ctgagcgaga acatcacggt ccccgacacc aaggtgaact tctacgcctg gaagcgcatg      240

gaggtggagg agcaggccat cgaggtctgg cagggcctgt ccctcctgag cgaggccatc      300

ctgcaggcgc aggccctcct ggccaactcc agccagcccc cggagacact gcagctccac      360

atcgacaagg ccatctccgg gctgcggagc ctgacctccc tcctgcgcgt gctgggcgcg      420

cagaaggagc tcatgagccc gcccgacacg acccccccgg ccccgctgcg gaccctgacc      480

gtggacacgt tctgcaagct cttccgcgtc tacgccaact tcctgcgggg caagctgaag      540

ctctacaccg gggaggtgtg ccgccggggc gaccgctga                             579


<210>  7
<211>  579
<212>  RNA
<213>  Mus musculus

<400>  7
augggggugc ccgaacgucc cacccugcug cuuuuacucu ccuugcuacu gauuccucug       60

ggccucccag uccucugugc ucccccacgc cucaucugcg acagucgagu ucuggagagg      120

uacaucuuag aggccaagga ggcagaaaau gucacgaugg guugugcaga aggucccaga      180

cugagugaaa auauuacagu cccagauacc aaagucaacu ucuaugcuug gaaaagaaug      240

gagguggaag aacaggccau agaaguuugg caaggccugu cccugcucuc agaagccauc      300

cugcaggccc aggcccugcu agccaauucc ucccagccac cagagacccu ucagcuucau      360

auagacaaag ccaucagugg ucuacguagc cucacuucac ugcuucgggu acugggagcu      420

cagaaggaau ugaugucgcc uccagauacc accccaccug cuccacuccg aacacucaca      480

guggauacuu ucugcaagcu cuuccggguc uacgccaacu uccuccgggg gaaacugaag      540

cuguacacgg gagaggucug caggagaggg gacagguga                             579


<210>  8
<211>  579
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  GC-rich sequence

<400>  8
augggcgucc ccgaaaggcc uacccugcug cugcuccugu cucugcuccu gaucccccug       60

ggacugcccg ugcugugcgc cccucccagg cugaucugcg acagcagggu gcuggaaaga      120

uacauccugg aagccaaaga ggccgagaac gucacaaugg gcugcgccga gggccccaga      180

cugagcgaga acaucaccgu gcccgacacc aaggucaacu ucuacgccug gaagaggaug      240

gaaguggagg aacaggccau cgaggucugg cagggacugu cucugcuguc cgaggccauc      300

cugcaggccc aggcucugcu ggccaauucu agccagcccc ccgagacacu gcagcugcac      360

aucgacaagg ccaucagcgg ccugagaagc cugaccagcc ugcugagggu gcugggagcc      420

cagaaagagc ugaugagccc cccugacacc acccccccug ccccccugag gacccugacc      480

guggacaccu ucugcaagcu guucagggug uacgccaacu uccugagggg caagcugaag      540

cuguacaccg gcgaggucug cagacggggc gacagauga                             579


<210>  9
<211>  579
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  A-rich sequence

<400>  9
augggagugc cagaaagacc aacccugcug cugcugcuca gccugcuacu gaucccacug       60

ggacucccag uccucugcgc accaccaaga cucaucugcg acagcagagu gcuggaaaga      120

uacauccuag aagcaaaaga agcagaaaac gucacgaugg gaugcgcaga aggaccaaga      180

cugagcgaaa acaucacagu cccagacacc aaagucaacu ucuacgcaug gaaaagaaug      240

gaaguggaag aacaggcaau agaagugugg caaggacuga gccugcucag cgaagcaauc      300

cugcaggcac aggcacugcu agcaaacagc agccagccac cagaaacccu gcagcugcac      360

auagacaaag caaucagcgg acuaagaagc cucaccagcc ugcugagagu acugggagca      420

cagaaagaac ugaugagccc accagacacc accccaccag caccacucag aacacucaca      480

guggacacuu ucugcaaacu cuucagaguc uacgcaaacu uccucagagg aaaacugaaa      540

cuguacacgg gagaagucug cagaagaggg gacagauga                             579


<210>  10
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  10
gcauaugacu                                                              10


<210>  11
<211>  206
<212>  PRT
<213>  Canis familiaris

<400>  11

Met Cys Glu Pro Ala Pro Pro Lys Pro Thr Gln Ser Ala Trp His Ser 
1               5                   10                  15      


Phe Pro Glu Cys Pro Ala Leu Leu Leu Leu Leu Ser Leu Leu Leu Leu 
            20                  25                  30          


Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Pro Arg Leu Ile Cys Asp 
        35                  40                  45              


Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Arg Glu Ala Glu Asn 
    50                  55                  60                  


Val Thr Met Gly Cys Ala Gln Gly Cys Ser Phe Ser Glu Asn Ile Thr 
65                  70                  75                  80  


Val Pro Asp Thr Lys Val Asn Phe Tyr Thr Trp Lys Arg Met Asp Val 
                85                  90                  95      


Gly Gln Gln Ala Leu Glu Val Trp Gln Gly Leu Ala Leu Leu Ser Glu 
            100                 105                 110         


Ala Ile Leu Arg Gly Gln Ala Leu Leu Ala Asn Ala Ser Gln Pro Ser 
        115                 120                 125             


Glu Thr Pro Gln Leu His Val Asp Lys Ala Val Ser Ser Leu Arg Ser 
    130                 135                 140                 


Leu Thr Ser Leu Leu Arg Ala Leu Gly Ala Gln Lys Glu Ala Met Ser 
145                 150                 155                 160 


Leu Pro Glu Glu Ala Ser Pro Ala Pro Leu Arg Thr Phe Thr Val Asp 
                165                 170                 175     


Thr Leu Cys Lys Leu Phe Arg Ile Tyr Ser Asn Phe Leu Arg Gly Lys 
            180                 185                 190         


Leu Thr Leu Tyr Thr Gly Glu Ala Cys Arg Arg Gly Asp Arg 
        195                 200                 205     


<210>  12
<211>  621
<212>  DNA
<213>  Canis familiaris

<400>  12
atgtgcgaac ccgccccacc taagcccact cagtctgctt ggcacagttt ccccgaatgt       60

ccagctctcc tgctgctgct ctccctgctg ctcctgcccc tcgggctgcc tgtgctgggc      120

gctcctccaa gactcatctg cgacagcagg gtgctggagc ggtacatcct ggaggctaga      180

gaagccgaga atgtcaccat ggggtgtgct cagggatgct ccttcagcga gaacatcacc      240

gtgcccgaca ctaaggtgaa cttctataca tggaagcgga tggatgtggg acagcaggcc      300

ctcgaagtgt ggcagggcct cgctctgctg tctgaagcca tcctgagggg acaggccctc      360

ctggctaatg ccagccagcc ttcagagacc ccccagctgc acgtggacaa agccgtgtca      420

agcctgagat ccctcacaag cctcctgagg gctctgggcg ctcagaagga agccatgtct      480

ctgccagagg aagccagccc tgccccactc aggaccttca ctgtcgatac cctgtgcaag      540

ctgttcagga tctattccaa ctttctgagg ggcaaactga cactctatac tggggaggct      600

tgtaggcggg gagaccgatg a                                                621


<210>  13
<211>  621
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A-rich sequence

<400>  13
atgtgcgaac cagcaccacc taaaccaaca cagagcgcat ggcacagctt cccagaatgc       60

ccagcactgc tgctgctgct cagcctgcta ctgctgccac tgggactccc agtcctcgga      120

gcaccaccaa gactcatctg cgacagcaga gtgctggaaa gatacatcct agaagcaaga      180

gaagcagaaa acgtcacgat gggatgcgca caaggatgca gcttcagcga aaacatcaca      240

gtcccagaca ccaaagtcaa cttctacaca tggaaaagaa tggacgtggg acagcaggca      300

ctggaagtgt ggcaaggact ggcactgctc agcgaagcaa tcctgagagg acaggcactg      360

ctagcaaacg caagccagcc aagcgaaacc ccacagctgc acgtagacaa agcagtgagc      420

agcctaagaa gcctcaccag cctgctgaga gcactgggag cacagaaaga agccatgagc      480

ctgccagaag aagccagccc agcaccactc agaacattca cagtggacac cctgtgcaaa      540

ctgttcagaa tatacagcaa cttcctcaga ggaaaactga cactgtacac gggagaagct      600

tgcagaggag gagacagatg a                                                621


<210>  14
<211>  621
<212>  RNA
<213>  Canis familiaris

<400>  14
augugcgaac ccgccccacc uaagcccacu cagucugcuu ggcacaguuu ccccgaaugu       60

ccagcucucc ugcugcugcu cucccugcug cuccugcccc ucgggcugcc ugugcugggc      120

gcuccuccaa gacucaucug cgacagcagg gugcuggagc gguacauccu ggaggcuaga      180

gaagccgaga augucaccau ggggugugcu cagggaugcu ccuucagcga gaacaucacc      240

gugcccgaca cuaaggugaa cuucuauaca uggaagcgga uggauguggg acagcaggcc      300

cucgaagugu ggcagggccu cgcucugcug ucugaagcca uccugagggg acaggcccuc      360

cuggcuaaug ccagccagcc uucagagacc ccccagcugc acguggacaa agccguguca      420

agccugagau cccucacaag ccuccugagg gcucugggcg cucagaagga agccaugucu      480

cugccagagg aagccagccc ugccccacuc aggaccuuca cugucgauac ccugugcaag      540

cuguucagga ucuauuccaa cuuucugagg ggcaaacuga cacucuauac uggggaggcu      600

uguaggcggg gagaccgaug a                                                621


<210>  15
<211>  621
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  A-rich sequence

<400>  15
augugcgaac cagcaccacc uaaaccaaca cagagcgcau ggcacagcuu cccagaaugc       60

ccagcacugc ugcugcugcu cagccugcua cugcugccac ugggacuccc aguccucgga      120

gcaccaccaa gacucaucug cgacagcaga gugcuggaaa gauacauccu agaagcaaga      180

gaagcagaaa acgucacgau gggaugcgca caaggaugca gcuucagcga aaacaucaca      240

gucccagaca ccaaagucaa cuucuacaca uggaaaagaa uggacguggg acagcaggca      300

cuggaagugu ggcaaggacu ggcacugcuc agcgaagcaa uccugagagg acaggcacug      360

cuagcaaacg caagccagcc aagcgaaacc ccacagcugc acguagacaa agcagugagc      420

agccuaagaa gccucaccag ccugcugaga gcacugggag cacagaaaga agccaugagc      480

cugccagaag aagccagccc agcaccacuc agaacauuca caguggacac ccugugcaaa      540

cuguucagaa uauacagcaa cuuccucaga ggaaaacuga cacuguacac gggagaagcu      600

ugcagaggag gagacagaug a                                                621


