                         SEQUENCE LISTING

<110>  The Hospital For Sick Children
 
<120>  Method and Compositions for Removing Duplicated Copy Number 
       Variations (CNVs) for Genetic Disorders and Related Uses

<130>  T8476320WO

<140>  Not Yet Assigned
<141>  2016-09-01

<150>  US 62/212,934
<151>  2015-09-01

<160>  107   

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1 dup 18-30 common forward

<400>  1
cagcatcatg acctgtttca atc                                               23


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2 dup 18-30 junc reverse

<400>  2
ttgtagaggg cagcaagttt gt                                                22


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P3 dup 18-30 bound reverse

<400>  3
aagaaggctg tcaccaggaa ct                                                22


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1 mecp2 dup common

<400>  4
cccacagagt agagtggagc ag                                                22


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2 mecp2 dup

<400>  5
ccacagccta tgaaaaggac aag                                               23


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P3 mecp2 dup out

<400>  6
ttagacagag tctcactcca tcacc                                             25


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMD S6141

<400>  7
atatcttctt aaatacccga                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMD S275

<400>  8
agtctcacaa aactgcagag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMD junc

<400>  9
tacttatgta ttttaaaaac                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X-Chromosome MECP2 Guide 75

<400>  10
gcttggccat ctaagttta                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X-Chromosome MECP2 Guide 80

<400>  11
gagttgtttg ggttaaacc                                                    19


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1 R

<400>  12
ggagaagatc cagaaccgta gtg                                               23


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2 F

<400>  13
cctgtcctgc tattattact ggatg                                             25


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P3 R

<400>  14
gctaaacgtt gaggctacag tttg                                              24


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i17-1

<400>  15
aatactacta gctcaccatc                                                   20


<210>  16
<211>  9178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpCas9(BB)-2A-Puro (PX459) V2.0_Guide i17-1

<400>  16
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg aatactacta gctcaccatc gttttagagc tagaaatagc aagttaaaat      300

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga      360

gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca      420

gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac      480

cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac      540

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata      600

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc      660

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta      720

ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc      780

cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg      840

gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc      900

ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga      960

ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga     1020

cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga     1080

ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat     1140

tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat     1200

tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact     1260

ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata     1320

agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga     1380

agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg     1440

agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca     1500

agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc     1560

tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag     1620

agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt     1680

ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg     1740

acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg     1800

acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt     1860

tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc     1920

tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca     1980

gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa     2040

atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc     2100

tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac     2160

tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg     2220

accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg     2280

acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga     2340

gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc     2400

ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg     2460

acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg     2520

acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga     2580

ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc     2640

ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc     2700

tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct     2760

ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca     2820

agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca     2880

acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc     2940

tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc     3000

agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc     3060

tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg     3120

aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca     3180

aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga     3240

cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg     3300

acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc     3360

ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga     3420

agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct     3480

ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca     3540

ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg     3600

tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg     3660

ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga     3720

tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca     3780

cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg     3840

tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc     3900

agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc     3960

ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc     4020

ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg     4080

agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa     4140

cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg     4200

acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt     4260

ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg     4320

cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc     4380

tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca     4440

agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga     4500

actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg     4560

agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc     4620

ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg     4680

gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga     4740

aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc     4800

tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc     4860

tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag     4920

ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt     4980

tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa     5040

acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga     5100

agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc     5160

actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg     5220

acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag     5280

agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct     5340

tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg     5400

acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc     5460

agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa     5520

aggaattcgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga     5580

atcctggccc aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc     5640

ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg     5700

tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg     5760

tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga     5820

ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg     5880

agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc     5940

ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca     6000

agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc     6060

ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca     6120

ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc     6180

ccggtgcctg agaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg     6240

ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc     6300

cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc     6360

tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag     6420

gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg     6480

cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg     6540

ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt     6600

tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg     6660

ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca     6720

cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc     6780

gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct     6840

ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg     6900

ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc     6960

ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg     7020

attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg     7080

aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct     7140

gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg     7200

gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg     7260

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc     7320

ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt     7380

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat     7440

ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg     7500

agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt     7560

tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga     7620

gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa     7680

gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt     7740

attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt     7800

gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc     7860

agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga     7920

ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat     7980

cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct     8040

gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc     8100

cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg     8160

gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc     8220

ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg     8280

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca     8340

ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta     8400

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     8460

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     8520

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     8580

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     8640

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     8700

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     8760

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     8820

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     8880

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     8940

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     9000

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     9060

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     9120

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt       9178


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i17-2

<400>  17
gcatggcgca aaggtcaaga                                                   20


<210>  18
<211>  9177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpCas9(BB)-2A-Puro (PX459) V2.0_Guide_i17-2

<400>  18
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg catggcgcaa aggtcaagag ttttagagct agaaatagca agttaaaata      300

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag      360

ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag      420

acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc      480

gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg      540

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      600

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca      660

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      720

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      780

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      840

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      900

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      960

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac     1020

gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac     1080

tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt     1140

agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt     1200

acctggagca cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggacta     1260

taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa     1320

gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa     1380

gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga     1440

gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa     1500

gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct     1560

gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga     1620

gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc     1680

cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga     1740

cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga     1800

cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt     1860

ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct     1920

gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag     1980

cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa     2040

tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct     2100

gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact     2160

gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga     2220

ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga     2280

catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag     2340

atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc     2400

tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga     2460

cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga     2520

cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac     2580

cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg     2640

gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct     2700

gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg     2760

gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa     2820

gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa     2880

cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct     2940

gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca     3000

gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct     3060

gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga     3120

agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa     3180

ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac     3240

actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga     3300

cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg     3360

gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa     3420

gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt     3480

taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat     3540

tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt     3600

ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc     3660

cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat     3720

cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac     3780

ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt     3840

ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca     3900

gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg     3960

gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg     4020

gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga     4080

gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac     4140

ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga     4200

cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc     4260

cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc     4320

ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct     4380

ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa     4440

gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa     4500

ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga     4560

gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg     4620

gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg     4680

cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa     4740

ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct     4800

ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct     4860

ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc     4920

caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt     4980

cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa     5040

cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa     5100

gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca     5160

ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga     5220

cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga     5280

gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt     5340

caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga     5400

cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca     5460

gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa     5520

ggaattcggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa     5580

tcctggccca atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc     5640

cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt     5700

cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt     5760

cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac     5820

cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga     5880

gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg     5940

gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa     6000

gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc     6060

cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac     6120

cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc     6180

cggtgcctga gaattctaac tagagctcgc tgatcagcct cgactgtgcc ttctagttgc     6240

cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc     6300

actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct     6360

attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg     6420

catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc     6480

gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg     6540

gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt     6600

ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc     6660

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     6720

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     6780

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     6840

tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc     6900

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     6960

tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga     7020

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     7080

attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg     7140

atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg     7200

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt     7260

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc     7320

tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc     7380

ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc     7440

cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga     7500

gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt     7560

ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag     7620

tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag     7680

aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta     7740

ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg     7800

agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca     7860

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag     7920

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc     7980

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg     8040

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc     8100

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg     8160

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg     8220

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga     8280

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac     8340

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa     8400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     8460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     8520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     8580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     8640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     8700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     8760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     8820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     8880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     8940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     9000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     9060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     9120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt        9177


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i30-1

<400>  19
actggtgaaa tcgtgcccgg                                                   20


<210>  20
<211>  9178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpCas9(BB)-2A-Puro (PX459) V2.0_Guide_i30-1

<400>  20
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg actggtgaaa tcgtgcccgg gttttagagc tagaaatagc aagttaaaat      300

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga      360

gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca      420

gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac      480

cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac      540

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata      600

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc      660

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta      720

ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc      780

cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg      840

gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc      900

ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga      960

ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga     1020

cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga     1080

ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat     1140

tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat     1200

tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact     1260

ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata     1320

agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga     1380

agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg     1440

agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca     1500

agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc     1560

tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag     1620

agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt     1680

ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg     1740

acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg     1800

acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt     1860

tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc     1920

tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca     1980

gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa     2040

atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc     2100

tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac     2160

tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg     2220

accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg     2280

acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga     2340

gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc     2400

ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg     2460

acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg     2520

acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga     2580

ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc     2640

ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc     2700

tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct     2760

ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca     2820

agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca     2880

acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc     2940

tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc     3000

agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc     3060

tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg     3120

aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca     3180

aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga     3240

cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg     3300

acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc     3360

ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga     3420

agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct     3480

ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca     3540

ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg     3600

tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg     3660

ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga     3720

tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca     3780

cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg     3840

tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc     3900

agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc     3960

ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc     4020

ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg     4080

agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa     4140

cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg     4200

acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt     4260

ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg     4320

cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc     4380

tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca     4440

agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga     4500

actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg     4560

agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc     4620

ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg     4680

gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga     4740

aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc     4800

tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc     4860

tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag     4920

ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt     4980

tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa     5040

acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga     5100

agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc     5160

actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg     5220

acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag     5280

agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct     5340

tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg     5400

acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc     5460

agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa     5520

aggaattcgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga     5580

atcctggccc aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc     5640

ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg     5700

tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg     5760

tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga     5820

ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg     5880

agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc     5940

ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca     6000

agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc     6060

ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca     6120

ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc     6180

ccggtgcctg agaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg     6240

ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc     6300

cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc     6360

tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag     6420

gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg     6480

cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg     6540

ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt     6600

tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg     6660

ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca     6720

cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc     6780

gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct     6840

ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg     6900

ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc     6960

ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg     7020

attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg     7080

aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct     7140

gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg     7200

gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg     7260

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc     7320

ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt     7380

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat     7440

ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg     7500

agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt     7560

tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga     7620

gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa     7680

gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt     7740

attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt     7800

gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc     7860

agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga     7920

ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat     7980

cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct     8040

gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc     8100

cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg     8160

gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc     8220

ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg     8280

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca     8340

ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta     8400

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     8460

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     8520

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     8580

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     8640

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     8700

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     8760

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     8820

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     8880

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     8940

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     9000

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     9060

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     9120

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt       9178


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i30-2

<400>  21
cttagaaaca ttcgtcaagg                                                   20


<210>  22
<211>  9178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpCas9(BB)-2A-Puro (PX459) V2.0_Guide_i30-2

<400>  22
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg cttagaaaca ttcgtcaagg gttttagagc tagaaatagc aagttaaaat      300

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga      360

gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca      420

gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac      480

cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac      540

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata      600

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc      660

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta      720

ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc      780

cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg      840

gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc      900

ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga      960

ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga     1020

cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga     1080

ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat     1140

tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat     1200

tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact     1260

ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata     1320

agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga     1380

agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg     1440

agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca     1500

agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc     1560

tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag     1620

agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt     1680

ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg     1740

acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg     1800

acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt     1860

tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc     1920

tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca     1980

gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa     2040

atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc     2100

tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac     2160

tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg     2220

accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg     2280

acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga     2340

gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc     2400

ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg     2460

acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg     2520

acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga     2580

ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc     2640

ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc     2700

tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct     2760

ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca     2820

agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca     2880

acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc     2940

tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc     3000

agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc     3060

tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg     3120

aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca     3180

aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga     3240

cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg     3300

acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc     3360

ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga     3420

agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct     3480

ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca     3540

ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg     3600

tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg     3660

ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga     3720

tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca     3780

cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg     3840

tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc     3900

agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc     3960

ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc     4020

ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg     4080

agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa     4140

cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg     4200

acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt     4260

ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg     4320

cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc     4380

tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca     4440

agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga     4500

actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg     4560

agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc     4620

ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg     4680

gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga     4740

aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc     4800

tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc     4860

tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag     4920

ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt     4980

tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa     5040

acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga     5100

agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc     5160

actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg     5220

acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag     5280

agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct     5340

tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg     5400

acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc     5460

agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa     5520

aggaattcgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga     5580

atcctggccc aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc     5640

ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg     5700

tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg     5760

tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga     5820

ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg     5880

agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc     5940

ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca     6000

agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc     6060

ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca     6120

ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc     6180

ccggtgcctg agaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg     6240

ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc     6300

cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc     6360

tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag     6420

gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg     6480

cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg     6540

ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt     6600

tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg     6660

ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca     6720

cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc     6780

gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct     6840

ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg     6900

ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc     6960

ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg     7020

attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg     7080

aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct     7140

gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg     7200

gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg     7260

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc     7320

ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt     7380

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat     7440

ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg     7500

agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt     7560

tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga     7620

gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa     7680

gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt     7740

attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt     7800

gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc     7860

agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga     7920

ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat     7980

cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct     8040

gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc     8100

cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg     8160

gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc     8220

ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg     8280

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca     8340

ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta     8400

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     8460

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     8520

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     8580

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     8640

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     8700

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     8760

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     8820

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     8880

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     8940

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     9000

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     9060

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     9120

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt       9178


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i21

<400>  23
gtgggatgga tccccggata                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i26

<400>  24
tagcatttga gttcaagcgt                                                   20


<210>  25
<211>  13013
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lentiCRISPR v2_Guide_i26

<400>  25
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg       60

atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt      120

gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc      180

tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac      240

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat      300

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg      360

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt      420

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag      480

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc      540

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag      600

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt      660

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc      720

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg      780

gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct      840

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt      900

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac      960

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     1020

gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     1080

ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     1140

ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     1200

ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     1260

aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     1320

tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     1380

caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     1440

aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     1500

aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg     1560

agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga     1620

gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata     1680

ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg     1740

acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg     1800

ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag     1860

ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt     1920

tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt     1980

aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt     2040

aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag     2100

aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata     2160

acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta     2220

agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta     2280

tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa     2340

gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt     2400

gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat     2460

tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa     2520

agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag     2580

agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg     2640

catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag     2700

atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta     2760

aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc     2820

ttggctttat atatcttgtg gaaaggacga aacaccgtag catttgagtt caagcgtgtt     2880

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc     2940

accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc     3000

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     3060

ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     3120

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     3180

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag     3240

cgctgccacc atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg     3300

ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa     3360

caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga     3420

aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa     3480

ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt     3540

cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc     3600

catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca     3660

cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc     3720

cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga     3780

caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga     3840

ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag     3900

caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct     3960

gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga     4020

cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa     4080

cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc     4140

cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct     4200

gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc     4260

tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa     4320

cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa     4380

gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga     4440

cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg     4500

agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg     4560

ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag     4620

gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa     4680

cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa     4740

cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta     4800

cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc     4860

cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg     4920

gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc     4980

cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct     5040

gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga     5100

agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa     5160

aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac     5220

cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa     5280

gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat     5340

ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg     5400

cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat     5460

cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga     5520

gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag     5580

ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa     5640

agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca     5700

gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga     5760

tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct     5820

gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa     5880

gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt     5940

cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat     6000

caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc     6060

ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac     6120

cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga     6180

gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct     6240

gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga     6300

cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt     6360

cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat     6420

ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg     6480

ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa     6540

gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga     6600

taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc     6660

caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact     6720

gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa     6780

tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa     6840

gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc     6900

cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta     6960

cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct     7020

gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc     7080

caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca     7140

ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa     7200

tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac     7260

cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga     7320

gacacggatc gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc     7380

tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac     7440

aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgaccgagta     7500

caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc     7560

cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga     7620

gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt     7680

gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc     7740

gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc     7800

cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt     7860

cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt     7920

gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc     7980

gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt     8040

gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt     8100

cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt     8160

tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc     8220

ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga     8280

gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc     8340

cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct     8400

ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg     8460

gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct     8520

gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc     8580

cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg     8640

tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact     8700

ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg     8760

ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg     8820

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg     8880

cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt     8940

gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg     9000

gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt     9060

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     9120

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg     9180

ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc     9240

ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca     9300

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc     9360

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac     9420

gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag     9480

tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc     9540

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg     9600

actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata     9660

agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa     9720

cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca     9780

gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc     9840

ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata     9900

gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg     9960

ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag    10020

ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg    10080

ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat    10140

agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca    10200

gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc    10260

ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg    10320

tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg    10380

tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact    10440

tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt    10500

tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac    10560

acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg    10620

ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg    10680

cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa    10740

atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca    10800

atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt    10860

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg    10920

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt    10980

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg    11040

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg    11100

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa    11160

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc    11220

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc    11280

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat    11340

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc    11400

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct    11460

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg    11520

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc    11580

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga    11640

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa    11700

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta    11760

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc    11820

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg    11880

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga    11940

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg    12000

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct    12060

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg    12120

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc    12180

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa    12240

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc    12300

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt    12360

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc    12420

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt    12480

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc    12540

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt    12600

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata    12660

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga    12720

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag    12780

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa    12840

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt    12900

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga    12960

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac           13013


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide i27

<400>  26
cccggtacag gggaacgcca                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i21 primer i21F

<400>  27
aggattgcag attgcttcag                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i21 primer i21R

<400>  28
ggtggagaga aaccagatgc                                                   20


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i21 primer i26 F

<400>  29
catttcactg ctctagtttt aatcctg                                           27


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i21 primer i26R

<400>  30
aaacacgctt gaactcaaat gctac                                             25


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i27 primer i27F

<400>  31
agtgaggtgc tctatgggaa atg                                               23


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA i27 primer i27R

<400>  32
ccatttaaga gtgggtaacc aagg                                              24


<210>  33
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i17-1

<400>  33
aauacuacua gcucaccauc                                                   20


<210>  34
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i17-2

<400>  34
gcauggcgca aaggucaaga                                                   20


<210>  35
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i30-1

<400>  35
acuggugaaa ucgugcccgg                                                   20


<210>  36
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i30-2

<400>  36
cuuagaaaca uucgucaagg                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i21

<400>  37
gugggaugga uccccggaua                                                   20


<210>  38
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i26

<400>  38
uagcauuuga guucaagcgu                                                   20


<210>  39
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA Guide i27

<400>  39
cccgguacag gggaacgcca                                                   20


<210>  40
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA DMD S6141

<400>  40
auaucuucuu aaauacccga                                                   20


<210>  41
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA DMD S275

<400>  41
agucucacaa aacugcagag                                                   20


<210>  42
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA DMD junc

<400>  42
uacuuaugua uuuuaaaaac                                                   20


<210>  43
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA X-Chromosome MECP2 Guide 75

<400>  43
gcuuggccau cuaaguuua                                                    19


<210>  44
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA X-Chromosome MECP2 Guide 80

<400>  44
gaguuguuug gguuaaacc                                                    19


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  45
gcttggccat ctaagtttac gg                                                22


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  46
aggttgccca tctaagttta tag                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  47
aagttggcct tctaagttta aag                                               23


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  48
tgaatgttca tctaagttta gag                                               23


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  49
ttcttgagcg tctaagttta agg                                               23


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  50
tggtagcccc tctaagttta agg                                               23


<210>  51
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  51
tgcttgacct cctaagttta aag                                               23


<210>  52
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  52
cattttgcca tctaagttta tgg                                               23


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  53
tacactgcca tctaagttta gag                                               23


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  54
ttcttggaag tctaagttta agg                                               23


<210>  55
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  55
tgatgtgcct tctaagttta aag                                               23


<210>  56
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  56
aacatggcca tctaagtttt ggg                                               23


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  57
tcgttggaca tttaagttta gag                                               23


<210>  58
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  58
agctgggcct tctaagtttg aag                                               23


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  59
ttcttggact tctaagtttg tgg                                               23


<210>  60
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  60
ttcatggaca tccaagttta tag                                               23


<210>  61
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  61
tgtttggccc tctaaattta agg                                               23


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  62
tgtttggcct tctaatttta agg                                               23


<210>  63
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  63
ttctagccca tctaagtttt cag                                               23


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  64
tgcaagccca tctaagtttt aag                                               23


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 1 Target Sequence with PAM

<400>  65
ttcttgtccc tctaagtttc tgg                                               23


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  66
gagttgtttg ggttaaacct gg                                                22


<210>  67
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  67
tgtgttgttt gggtgaaacc gag                                               23


<210>  68
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  68
tgagtttttt gggttaagcc tgg                                               23


<210>  69
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  69
actgttcttt gggttaaacc aag                                               23


<210>  70
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  70
agttttgttt tggttaaacc tag                                               23


<210>  71
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  71
tgagctattt ggtttaaacc aag                                               23


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  72
gcagatgttt ggattaaacc aag                                               23


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  73
gaagatgttt gggttaaaac tag                                               23


<210>  74
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  74
tgaattcctt tggttaaacc cag                                               23


<210>  75
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  75
tgaggtctca gggttaaacc aag                                               23


<210>  76
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  76
tcatttcttt gggttaaaca tag                                               23


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  77
tgagatgatg gggttaaagc tgg                                               23


<210>  78
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  78
ttattggttt gagttaaacc aag                                               23


<210>  79
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  79
agggtttttt ggcttaaacc tag                                               23


<210>  80
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  80
tggattgttt ttgttaaacc tgg                                               23


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  81
tgactagttg gggttaaaca tgg                                               23


<210>  82
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  82
tgagttgaaa gggttaaaca cag                                               23


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  83
tttgttcttt gggttaaagc cag                                               23


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  84
tgagccatat gggttaaacc agg                                               23


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  85
ttagtcattt cggttaaacc agg                                               23


<210>  86
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MECP2: sgRNA 2 Target Sequence with PAM

<400>  86
agaggtgtgt gggttaaagc agg                                               23


<210>  87
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  87
atatcttctt aaatacccga agg                                               23


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  88
agtgtcttct taaatacctg cag                                               23


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  89
aaaaccttca caaatacccg gag                                               23


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  90
aatctcttct tcaataccct tgg                                               23


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  91
aagagctgct taaataccct gag                                               23


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  92
catatcttct taaatagcct tgg                                               23


<210>  93
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  93
attagcatct ttaatacccg aag                                               23


<210>  94
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  94
gatatatcct gaaatacccg tag                                               23


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  95
attttcttat gaaatacccg aag                                               23


<210>  96
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  96
taaatcctct taaataccct aag                                               23


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  97
gaaatcttca taaataccag gag                                               23


<210>  98
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  98
aattacttca taaatacctg agg                                               23


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  99
aatttcaact taaataccct tgg                                               23


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  100
aattccatct taaataccct aag                                               23


<210>  101
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  101
catcttttct taaataccca agg                                               23


<210>  102
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  102
agtttcttgt taaataccca agg                                               23


<210>  103
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  103
attctctttt taaataccca cag                                               23


<210>  104
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  104
aatttctctt taaataccca aag                                               23


<210>  105
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  105
aatattttct tcaatacccc tgg                                               23


<210>  106
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  106
aatattttct tcaataccct aag                                               23


<210>  107
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dup18-30: sgRNA 1 Target Sequence with PAM

<400>  107
aataccttca taagtacccg aag                                               23


