                         SEQUENCE LISTING

<110>  New York University
       Sigurdsson, Einar M
 
<120>  Antibody-Based Molecules Selective for the {p}Ser404 Epitope of 
       Tau and Their Uses in the Diagnosis and Treatment of Tauopathy

<130>  1400.0009PCT

<150>  US 62/204,711
<151>  2015-08-13

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  441
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(441)
<223>  Human Tau residues

<400>  1

Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      


Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          


Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Glu Ser Pro Leu 
        35                  40                  45              


Gln Thr Pro Thr Glu Asp Gly Ser Glu Glu Pro Gly Ser Glu Thr Ser 
    50                  55                  60                  


Asp Ala Lys Ser Thr Pro Thr Ala Glu Asp Val Thr Ala Pro Leu Val 
65                  70                  75                  80  


Asp Glu Gly Ala Pro Gly Lys Gln Ala Ala Ala Gln Pro His Thr Glu 
                85                  90                  95      


Ile Pro Glu Gly Thr Thr Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro 
            100                 105                 110         


Ser Leu Glu Asp Glu Ala Ala Gly His Val Thr Gln Ala Arg Met Val 
        115                 120                 125             


Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp Asp Lys Lys Ala Lys Gly 
    130                 135                 140                 


Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro Arg Gly Ala Ala Pro Pro 
145                 150                 155                 160 


Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile Pro Ala Lys Thr Pro 
                165                 170                 175     


Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly Glu Pro Pro Lys Ser Gly 
            180                 185                 190         


Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser 
        195                 200                 205             


Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys 
    210                 215                 220                 


Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser Pro Ser Ser Ala Lys 
225                 230                 235                 240 


Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val 
                245                 250                 255     


Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly 
            260                 265                 270         


Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln 
        275                 280                 285             


Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
    290                 295                 300                 


Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser 
305                 310                 315                 320 


Lys Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln 
                325                 330                 335     


Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser 
            340                 345                 350         


Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn 
        355                 360                 365             


Lys Lys Ile Glu Thr His Lys Leu Thr Phe Arg Glu Asn Ala Lys Ala 
    370                 375                 380                 


Lys Thr Asp His Gly Ala Glu Ile Val Tyr Lys Ser Pro Val Val Ser 
385                 390                 395                 400 


Gly Asp Thr Ser Pro Arg His Leu Ser Asn Val Ser Ser Thr Gly Ser 
                405                 410                 415     


Ile Asp Met Val Asp Ser Pro Gln Leu Ala Thr Leu Ala Asp Glu Val 
            420                 425                 430         


Ser Ala Ser Leu Ala Lys Gln Gly Leu 
        435                 440     


<210>  2
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-Containing Linker

<400>  2

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  3

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  4
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Preferred Linker

<400>  4

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
1               5                   10                  15      


Ser Ser 
        


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Tag

<400>  5

His His His His His His 
1               5       


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-Tag

<400>  6

Gly Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser 
1               5                   10          


<210>  7
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunogen containing the {P}Ser404 Epitope


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  PHOSPHORYLATION (Residue is Phosphoserine)

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  PHOSPHORYLATION (Residue is Phosphoserine)

<400>  7

Thr Asp His Gly Ala Glu Ile Val Tyr Lys Ser Pro Val Val Ser Gly 
1               5                   10                  15      


Asp Thr Ser Pro Arg His Leu 
            20              


<210>  8
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunogen containing the {P}Ser404 Epitope


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  PHOSPHORYLATION (Residue is Phosphoserine)

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  PHOSPHORYLATION (Residue is Phosphoserine)

<400>  8

Thr Asp His Gly Ala Glu Ile Val Tyr Lys Ser Pro Val Val Ser Gly 
1               5                   10                  15      


Asp Thr Ser Pro Arg His Leu 
            20              


<210>  9
<211>  108
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(108)
<223>  Light Chain Variable Domain of Antibody 4E6G7

<400>  9

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  10
<211>  11
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(11)
<223>  CDR1 of Light Chain of Antibody 4E6G7

<400>  10

His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser 
1               5                   10      


<210>  11
<211>  7
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  CDR2 of Light Chain of Antibody 4E6G7

<400>  11

Glu Ala Ser Thr Leu Tyr Thr 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  CDR3 of Light Chain of Antibody 4E6G7

<400>  12

Gln Gln Gly Gln Ser Tyr Pro Trp Thr 
1               5                   


<210>  13
<211>  112
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  Heavy Chain Variable Domain of Antibody 4E6G7

<400>  13

Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser 
            20                  25                  30          


Met His Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Arg Ile Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln 
    50                  55                  60                  


Gly Lys Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu 
65                  70                  75                  80  


Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser 
                85                  90                  95      


Gly Ser Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


<210>  14
<211>  10
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(10)
<223>  CDR1 of Heavy Chain of Antibody 4E6G7

<400>  14

Gly Phe Asn Ile Lys Asp Thr Ser Met His 
1               5                   10  


<210>  15
<211>  17
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(17)
<223>  CDR2 of Heavy Chain of Antibody 4E6G7

<400>  15

Arg Ile Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  16
<211>  5
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  CDR3 of Heavy Chain of Antibody 4E6G7

<400>  16

Ser Gly Asn Tyr Asp 
1               5   


<210>  17
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(108)
<223>  Light Chain Variable Domain of Antibody 4E6G7

<220>
<221>  MISC_FEATURE
<222>  (109)..(126)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (127)..(238)
<223>  Heavy Chain Variable Domain of Antibody 4E6G7

<400>  17

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His 
145                 150                 155                 160 


Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile 
                165                 170                 175     


Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys 
            180                 185                 190         


Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
        195                 200                 205             


Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser 
    210                 215                 220                 


Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
225                 230                 235             


<210>  18
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-Terminal Leader Peptide

<400>  18

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala 
            20                  25              


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-Terminal Peptide

<400>  19

Ala Lys Thr Thr Pro Pro Ser Val Thr Ser Gly Gln Ala Gly Gln 
1               5                   10                  15  


<210>  20
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-Terminal Peptide

<400>  20

Ala Lys Thr Thr Pro Pro Ser Val Thr Ser Gly Gln Ala Gly Gln His 
1               5                   10                  15      


His His His His His Gly Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
            20                  25                  30          


Ser 
    


<210>  21
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(28)
<223>  N-Terminal Leader Peptide (SEQ ID NO:18)

<220>
<221>  MISC_FEATURE
<222>  (29)..(266)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<400>  21

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala Asp Ile Gln Met 
            20                  25                  30          


Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Thr Ile Thr 
        35                  40                  45              


Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile Phe Glu Ala Ser 
65                  70                  75                  80  


Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln Leu Gln Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys Leu Ser Cys Thr 
                165                 170                 175     


Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His Trp Val Arg Gln 
            180                 185                 190         


Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Ala Pro Ala Asn 
        195                 200                 205             


Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser Gly Asn Tyr Asp 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            260                 265     


<210>  22
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(238)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (239)..(253)
<223>  C-Terminal Peptide (SEQ ID NO:19)

<400>  22

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His 
145                 150                 155                 160 


Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile 
                165                 170                 175     


Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys 
            180                 185                 190         


Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
        195                 200                 205             


Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser 
    210                 215                 220                 


Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ala Lys 
225                 230                 235                 240 


Thr Thr Pro Pro Ser Val Thr Ser Gly Gln Ala Gly Gln 
                245                 250             


<210>  23
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(238)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (239)..(244)
<223>  C-Terminal Peptide (SEQ ID NO:5)

<400>  23

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His 
145                 150                 155                 160 


Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile 
                165                 170                 175     


Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys 
            180                 185                 190         


Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
        195                 200                 205             


Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser 
    210                 215                 220                 


Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser His His 
225                 230                 235                 240 


His His His His 
                


<210>  24
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(238)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (239)..(250)
<223>  C-Terminal Peptide (SEQ ID NO:6)

<400>  24

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His 
145                 150                 155                 160 


Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile 
                165                 170                 175     


Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys 
            180                 185                 190         


Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
        195                 200                 205             


Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser 
    210                 215                 220                 


Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Gly Ala 
225                 230                 235                 240 


Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser 
                245                 250 


<210>  25
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(238)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (239)..(271)
<223>  C-Terminal Peptide (SEQ ID NO:20)

<400>  25

Asp Ile Gln Met Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Phe Glu Ala Ser Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His 
145                 150                 155                 160 


Trp Val Arg Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile 
                165                 170                 175     


Ala Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys 
            180                 185                 190         


Ala Thr Ile Thr Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
        195                 200                 205             


Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser 
    210                 215                 220                 


Gly Asn Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ala Lys 
225                 230                 235                 240 


Thr Thr Pro Pro Ser Val Thr Ser Gly Gln Ala Gly Gln His His His 
                245                 250                 255     


His His His Gly Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser 
            260                 265                 270     


<210>  26
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(28)
<223>  N-Terminal Leader Peptide (SEQ ID NO:18)

<220>
<221>  MISC_FEATURE
<222>  (29)..(266)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (267)..(281)
<223>  C-Terminal Peptide (SEQ ID NO:5)

<400>  26

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala Asp Ile Gln Met 
            20                  25                  30          


Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Thr Ile Thr 
        35                  40                  45              


Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile Phe Glu Ala Ser 
65                  70                  75                  80  


Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln Leu Gln Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys Leu Ser Cys Thr 
                165                 170                 175     


Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His Trp Val Arg Gln 
            180                 185                 190         


Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Ala Pro Ala Asn 
        195                 200                 205             


Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser Gly Asn Tyr Asp 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ala Lys Thr Thr Pro Pro 
            260                 265                 270         


Ser Val Thr Ser Gly Gln Ala Gly Gln 
        275                 280     


<210>  27
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(28)
<223>  N-Terminal Leader Peptide (SEQ ID NO:18)

<220>
<221>  MISC_FEATURE
<222>  (29)..(266)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (267)..(272)
<223>  C-Terminal Peptide (SEQ ID NO:18)

<400>  27

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala Asp Ile Gln Met 
            20                  25                  30          


Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Thr Ile Thr 
        35                  40                  45              


Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile Phe Glu Ala Ser 
65                  70                  75                  80  


Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln Leu Gln Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys Leu Ser Cys Thr 
                165                 170                 175     


Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His Trp Val Arg Gln 
            180                 185                 190         


Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Ala Pro Ala Asn 
        195                 200                 205             


Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser Gly Asn Tyr Asp 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser His His His His His His 
            260                 265                 270         


<210>  28
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(28)
<223>  N-Terminal Leader Peptide (SEQ ID NO:18)

<220>
<221>  MISC_FEATURE
<222>  (29)..(266)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (267)..(278)
<223>  C-Terminal Peptide (SEQ ID NO:6)

<400>  28

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala Asp Ile Gln Met 
            20                  25                  30          


Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Thr Ile Thr 
        35                  40                  45              


Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile Phe Glu Ala Ser 
65                  70                  75                  80  


Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln Leu Gln Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys Leu Ser Cys Thr 
                165                 170                 175     


Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His Trp Val Arg Gln 
            180                 185                 190         


Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Ala Pro Ala Asn 
        195                 200                 205             


Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser Gly Asn Tyr Asp 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Gly Ala Tyr Pro Tyr Asp 
            260                 265                 270         


Val Pro Asp Tyr Ala Ser 
        275             


<210>  29
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody 4E6G7 scFv Fusion Protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(28)
<223>  N-Terminal Leader Peptide (SEQ ID NO:18)

<220>
<221>  MISC_FEATURE
<222>  (29)..(266)
<223>  Antibody 4E6G7 scFv Fusion Protein (SEQ ID NO:17)

<220>
<221>  MISC_FEATURE
<222>  (267)..(299)
<223>  C-Terminal Peptide (SEQ ID NO:20)

<400>  29

Ile Gln Glu Glu Phe Lys Met Lys Lys Thr Ala Ile Ala Ile Ala Val 
1               5                   10                  15      


Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala Ala Asp Ile Gln Met 
            20                  25                  30          


Asn Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Thr Ile Thr 
        35                  40                  45              


Ile Ser Cys His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile Phe Glu Ala Ser 
65                  70                  75                  80  


Thr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
            100                 105                 110         


Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Val Gln Leu Gln Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Leu Val Gln Pro Gly Ala Ser Val Lys Leu Ser Cys Thr 
                165                 170                 175     


Ala Ser Gly Phe Asn Ile Lys Asp Thr Ser Met His Trp Val Arg Gln 
            180                 185                 190         


Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Ala Pro Ala Asn 
        195                 200                 205             


Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Thr Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ser Gly Ser Gly Asn Tyr Asp 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ala Lys Thr Thr Pro Pro 
            260                 265                 270         


Ser Val Thr Ser Gly Gln Ala Gly Gln His His His His His His Gly 
        275                 280                 285             


Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser 
    290                 295                 


<210>  30
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tau 379-408 ({p}Ser396/{p}Ser404)


<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  PHOSPHORYLATION (residue is phosphoserine)

<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  PHOSPHORYLATION (residue is phosphoserine)

<400>  30

Arg Glu Asn Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val Tyr 
1               5                   10                  15      


Lys Ser Pro Val Val Ser Gly Asp Thr Ser Pro Arg His Leu 
            20                  25                  30  


<210>  31
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  Tau 379-408

<400>  31

Arg Glu Asn Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val Tyr 
1               5                   10                  15      


Lys Ser Pro Val Val Ser Gly Asp Thr Ser Pro Arg His Leu 
            20                  25                  30  


