                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
 
<120>  Method for producing an animal comprising a germline genetic 
       modification

<130>  523041

<150>  2015903164
<151>  2015-08-07

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  839
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region of inverted_pT2 plasmid used to generate GFP birds

<400>  1
tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc       60

tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaccacc      120

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      180

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      240

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccaca      300

ctagtgacca ccttcgctta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      360

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      420

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      480

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      540

aagctggagt acaacttcaa cagccacaac gtatacatca tggccgacaa gcagaagaac      600

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      660

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      720

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      780

ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagta       839


<210>  2
<211>  763
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region corresponding to SEQ ID NO:1 from I_30 which is a GFP 
       expressing embryo

<400>  2
ggtcctgggc acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaccac       60

catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga      120

cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta      180

cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac      240

actagtgacc accttcgctt acggcgtgca gtgcttcagc cgctaccccg accacatgaa      300

gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt      360

cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct      420

ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca      480

caagctggag tacaacttca acagccacaa cgtatacatc atggccgaca agcagaagaa      540

cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc      600

cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca      660

ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt      720

cctgctggag ttcgtgaccg ccgccgggat cactccgggc atg                        763


<210>  3
<211>  758
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region corresponding to SEQ ID NO:1 from I_10 which is a GFP PCR 
       positive embryo that has a WT phenotype

<400>  3
cgtgctggtt attgtgctgt ctcatcattt tggcagagaa ttgtaccacc gtggtgagca       60

agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa      120

acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga      180

ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccaca ctagtgacca      240

ccttcgctta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact      300

tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg      360

acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca      420

tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt      480

acaacttcaa cagccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg      540

tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc      600

agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca      660

cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt      720

tcgtgaccgc cgccgggatc actcacgggc atggacga                              758


<210>  4
<211>  766
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region corresponding to SEQ ID NO:1 from I_15 which is a GFP PCR 
       positive embryo that has a WT phenotype

<400>  4
tcgtgggcac gtgctggtta ttgtgctgtc tcatcatttt ggcagagaat tgtaccaccg       60

tggtgagcaa gggcgaggag ctggtcaccg gggtggtgcc catcctggtc gagctggacg      120

gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg      180

gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccacac      240

tagtgaccac cttcgcttac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc      300

agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct      360

tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg      420

tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca      480

agctggagta caacttcaac agccacaacg tatacatcat ggccgacaag cagaagaacg      540

gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg      600

accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact      660

acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc      720

tgctggagtt cgtgaccgcc gccgggatca ctcacggcat ggacga                     766


<210>  5
<211>  748
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region corresponding to SEQ ID NO:1 from I_23 which is a GFP PCR 
       positive embryo that has a WT phenotype

<400>  5
cgtgcgggtt attgggctgt ctcatcgttt tggcagagaa gtgtaccacc gtggtgagca       60

agggcgagga gctggtcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa      120

acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga      180

ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccaca ctagtgacca      240

ccttcgctta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact      300

tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg      360

acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca      420

tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt      480

acaacttcaa cagccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg      540

tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc      600

agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca      660

cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt      720

tcgtgaccgc cgccgggatc actcacgg                                         748


<210>  6
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA sg-GFP-1

<400>  6
gcagcggcag gucgagcugg ucc                                               23


<210>  7
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA sg-GFP-2

<400>  7
ccucgcgugg uagaagaagu ucc                                               23


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  8
agcctctgct aaccatgttc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  9
cgtccatgcc gtgagtgatc                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  10
caacacagtg ctgtctggtg g                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  11
atcgtactcc tgcttgctga t                                                 21


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMRT1 PCT Primer FWD

<400>  12
agcaagccca ggaagaggag                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMRT1 PCR Primer REV

<400>  13
gttccagtgt agtgcaggag                                                   20


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sex test PCR Primer FWD

<400>  14
cccaaatata acacgcttca ct                                                22


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sex test PCR Primer REV

<400>  15
gaaatgaatt attttctggc gac                                               23


<210>  16
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DMRT1 CRISPR guide 1

<400>  16
cgucgucgcu cgcguggugg ucc                                               23


<210>  17
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DMRT1 CRISPR guide 2

<400>  17
cuuuguacug ccugauugug acc                                               23


<210>  18
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMRT1 HDR oligo

<400>  18
ctgctcactc cacgagcacg gtggcagcag cagcagcgag cgctagtcta gactagtcag       60

tgccagtgag gggcccactg ctccttgctg cccaggcag                              99


