                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING MANNANASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  13054-EP-EPA

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2157
<212>  DNA
<213>  Neobulgaria  sp.


<220>
<221>  CDS
<222>  (1)..(2154)

<220>
<221>  sig_peptide
<222>  (1)..(57)

<220>
<221>  mat_peptide
<222>  (58)..(2154)

<400>  1
atg cgt tca ctt tct tcc ctg acg ttg ctg ctg ggc atc gca tcc tca         48
Met Arg Ser Leu Ser Ser Leu Thr Leu Leu Leu Gly Ile Ala Ser Ser           
                -15                 -10                 -5                

gta tcc tcg aca gta ttt gaa gcc gaa aag ggt acc ctc gct ggt ggc         96
Val Ser Ser Thr Val Phe Glu Ala Glu Lys Gly Thr Leu Ala Gly Gly           
        -1  1               5                   10                        

cta acg att gca aca gat gta acc gga tat acc gga acc gga tat gtc        144
Leu Thr Ile Ala Thr Asp Val Thr Gly Tyr Thr Gly Thr Gly Tyr Val           
    15                  20                  25                            

acg aac ttc gcc gat gct gct gct ttg ttg aca ttt act gtc aat gga        192
Thr Asn Phe Ala Asp Ala Ala Ala Leu Leu Thr Phe Thr Val Asn Gly           
30                  35                  40                  45            

ttg acc gct gga agc tat gat ttg acc ctt aca tac agt gca cag tat        240
Leu Thr Ala Gly Ser Tyr Asp Leu Thr Leu Thr Tyr Ser Ala Gln Tyr           
                50                  55                  60                

ggg gac aag ttc act act gta tcc gtc aat ggg gcc tct ggt att gaa        288
Gly Asp Lys Phe Thr Thr Val Ser Val Asn Gly Ala Ser Gly Ile Glu           
            65                  70                  75                    

gtt gcc atc acc aat gtc aca acc gca acc tgg aca aca gcc acc att        336
Val Ala Ile Thr Asn Val Thr Thr Ala Thr Trp Thr Thr Ala Thr Ile           
        80                  85                  90                        

ggt acc ttc aca ttg acc gct gga gac aat aca gtc agt tgc gcc gac        384
Gly Thr Phe Thr Leu Thr Ala Gly Asp Asn Thr Val Ser Cys Ala Asp           
    95                  100                 105                           

gat tgg gga tgg tat ttg att gat tct ctc acc gtt atc ccc acc cct        432
Asp Trp Gly Trp Tyr Leu Ile Asp Ser Leu Thr Val Ile Pro Thr Pro           
110                 115                 120                 125           

gcc aaa ccc atc aca atc gtc gat gtc tcc aac gga gcc acc gca caa        480
Ala Lys Pro Ile Thr Ile Val Asp Val Ser Asn Gly Ala Thr Ala Gln           
                130                 135                 140               

gcc gaa gat gga att ctg act ggt act act gtc gga act act act gcc        528
Ala Glu Asp Gly Ile Leu Thr Gly Thr Thr Val Gly Thr Thr Thr Ala           
            145                 150                 155                   

gga tat aca gga act gga tac gtt aca gga ttc acc gcc acc gga act        576
Gly Tyr Thr Gly Thr Gly Tyr Val Thr Gly Phe Thr Ala Thr Gly Thr           
        160                 165                 170                       

caa gtg acc atc aac ttg tct agc act aag caa gca tta tac gat gtt        624
Gln Val Thr Ile Asn Leu Ser Ser Thr Lys Gln Ala Leu Tyr Asp Val           
    175                 180                 185                           

gta gtc cgc tat gct gcc atc tac gga caa aaa tac acc aca atg caa        672
Val Val Arg Tyr Ala Ala Ile Tyr Gly Gln Lys Tyr Thr Thr Met Gln           
190                 195                 200                 205           

cta aat ggt gtt gga ggg tca gag att ctt ctt ctt gat acc acc act        720
Leu Asn Gly Val Gly Gly Ser Glu Ile Leu Leu Leu Asp Thr Thr Thr           
                210                 215                 220               

gcg act tct cca tgg gca aat gcc act gca gga caa gta ttg ttg gcg        768
Ala Thr Ser Pro Trp Ala Asn Ala Thr Ala Gly Gln Val Leu Leu Ala           
            225                 230                 235                   

agc gga aat aac acc ctc aca ttc atg aat gat tgg ggc tgg tat ttc        816
Ser Gly Asn Asn Thr Leu Thr Phe Met Asn Asp Trp Gly Trp Tyr Phe           
        240                 245                 250                       

att gat gcc gtc tac gtt aca cct tct cca gct cca gca cca cac aaa        864
Ile Asp Ala Val Tyr Val Thr Pro Ser Pro Ala Pro Ala Pro His Lys           
    255                 260                 265                           

gtg acc aat gcg ctc gtc gat gcg aag gca cta gca agc aca cac gcc        912
Val Thr Asn Ala Leu Val Asp Ala Lys Ala Leu Ala Ser Thr His Ala           
270                 275                 280                 285           

ctt ttc aac act ctc ctc gcc aag tat ggt agt gga gat att ttc tct        960
Leu Phe Asn Thr Leu Leu Ala Lys Tyr Gly Ser Gly Asp Ile Phe Ser           
                290                 295                 300               

ggg caa gca gac ccc act gga gta aca tgg att gag tca aat ctt gga       1008
Gly Gln Ala Asp Pro Thr Gly Val Thr Trp Ile Glu Ser Asn Leu Gly           
            305                 310                 315                   

aca aca aag aca ccc gca atc att gga ctt gac atg att gaa tac agt       1056
Thr Thr Lys Thr Pro Ala Ile Ile Gly Leu Asp Met Ile Glu Tyr Ser           
        320                 325                 330                       

ccc aca cgt gtt ctg tac gga tct acc agc aca gct gtc gaa gac gcc       1104
Pro Thr Arg Val Leu Tyr Gly Ser Thr Ser Thr Ala Val Glu Asp Ala           
    335                 340                 345                           

att gca ttt gat aaa cgt ggt ggt atg gtc gcc ttc caa tgg cat tgg       1152
Ile Ala Phe Asp Lys Arg Gly Gly Met Val Ala Phe Gln Trp His Trp           
350                 355                 360                 365           

aat gca cca gct gac ctc atc aac aac gat acc gtt cca tgg tgg aag       1200
Asn Ala Pro Ala Asp Leu Ile Asn Asn Asp Thr Val Pro Trp Trp Lys           
                370                 375                 380               

gga ttc tac tct tac ggc aca aca ttc aac ttg acc gct gcc ctt gcc       1248
Gly Phe Tyr Ser Tyr Gly Thr Thr Phe Asn Leu Thr Ala Ala Leu Ala           
            385                 390                 395                   

aat ccc tca ggg agc gat tat gcc ctc ctc atc tca gat atg gat gcc       1296
Asn Pro Ser Gly Ser Asp Tyr Ala Leu Leu Ile Ser Asp Met Asp Ala           
        400                 405                 410                       

atc gct gtc caa ctt ctc cgc ctc caa gca gcc ggc gtc cca gtt ctc       1344
Ile Ala Val Gln Leu Leu Arg Leu Gln Ala Ala Gly Val Pro Val Leu           
    415                 420                 425                           

tgg aga cca ctt cac gaa gcc gat gga aca tgg ttc tgg tgg ggt aat       1392
Trp Arg Pro Leu His Glu Ala Asp Gly Thr Trp Phe Trp Trp Gly Asn           
430                 435                 440                 445           

ttc gga gca gct tcc tgt gtt tct ctt tac aga atc atg tac gat cga       1440
Phe Gly Ala Ala Ser Cys Val Ser Leu Tyr Arg Ile Met Tyr Asp Arg           
                450                 455                 460               

tac acc aac tac cac gga ctc cac aac ctc atc tgg gtc tgg aac tcc       1488
Tyr Thr Asn Tyr His Gly Leu His Asn Leu Ile Trp Val Trp Asn Ser           
            465                 470                 475                   

gtc act cca tcc tgg tac ccc ggt gcg gat gtc gtt gat atc ctc ggt       1536
Val Thr Pro Ser Trp Tyr Pro Gly Ala Asp Val Val Asp Ile Leu Gly           
        480                 485                 490                       

tat gat tcc tac cca gca gtt gga gat cac ggt cca gta tca agt caa       1584
Tyr Asp Ser Tyr Pro Ala Val Gly Asp His Gly Pro Val Ser Ser Gln           
    495                 500                 505                           

tat aac gct ttg att acc ctc ggt gga gac acc aag cta gtc aca ctt       1632
Tyr Asn Ala Leu Ile Thr Leu Gly Gly Asp Thr Lys Leu Val Thr Leu           
510                 515                 520                 525           

cca gaa gtt gga aac att ccc gac cca gcc atc ctc aag ttg tac cat       1680
Pro Glu Val Gly Asn Ile Pro Asp Pro Ala Ile Leu Lys Leu Tyr His           
                530                 535                 540               

gca gac tgg agt tac ttc gtc act tgg aat caa gac tat atc ctc acg       1728
Ala Asp Trp Ser Tyr Phe Val Thr Trp Asn Gln Asp Tyr Ile Leu Thr           
            545                 550                 555                   

gat acc tat aac cca ctt gca ttc aaa caa caa gtc tac aac gat ccc       1776
Asp Thr Tyr Asn Pro Leu Ala Phe Lys Gln Gln Val Tyr Asn Asp Pro           
        560                 565                 570                       

aca gtc ctt aaa ctt acc gat ctt gga aat tgg aag ggg gca gct act       1824
Thr Val Leu Lys Leu Thr Asp Leu Gly Asn Trp Lys Gly Ala Ala Thr           
    575                 580                 585                           

tcg act att gtt tcc tca aca tcg aaa gtt tca acc act act tcg agt       1872
Ser Thr Ile Val Ser Ser Thr Ser Lys Val Ser Thr Thr Thr Ser Ser           
590                 595                 600                 605           

ttg atc aca tcg aca acc aag aag acc tct tca tca act gtt gtt tca       1920
Leu Ile Thr Ser Thr Thr Lys Lys Thr Ser Ser Ser Thr Val Val Ser           
                610                 615                 620               

act aca agt tcg acg gtc aag acc aca tca acc aca tca aag gta tca       1968
Thr Thr Ser Ser Thr Val Lys Thr Thr Ser Thr Thr Ser Lys Val Ser           
            625                 630                 635                   

tct agc acc aca aag gtg tct agc act aca aag gtt aca aca aca tcc       2016
Ser Ser Thr Thr Lys Val Ser Ser Thr Thr Lys Val Thr Thr Thr Ser           
        640                 645                 650                       

acc act act agt gct gtt gct acc gcc aca gct ggc cac tgg ggg caa       2064
Thr Thr Thr Ser Ala Val Ala Thr Ala Thr Ala Gly His Trp Gly Gln           
    655                 660                 665                           

tgt gga gga act gga tgg acc ggt cca act gtc tgt gca tca gga ttt       2112
Cys Gly Gly Thr Gly Trp Thr Gly Pro Thr Val Cys Ala Ser Gly Phe           
670                 675                 680                 685           

act tgc act gct gtc tcc cca cca tac tat tat caa tgt ttg tag           2157
Thr Cys Thr Ala Val Ser Pro Pro Tyr Tyr Tyr Gln Cys Leu                   
                690                 695                                   


<210>  2
<211>  718
<212>  PRT
<213>  Neobulgaria  sp.

<400>  2

Met Arg Ser Leu Ser Ser Leu Thr Leu Leu Leu Gly Ile Ala Ser Ser 
                -15                 -10                 -5      


Val Ser Ser Thr Val Phe Glu Ala Glu Lys Gly Thr Leu Ala Gly Gly 
        -1  1               5                   10              


Leu Thr Ile Ala Thr Asp Val Thr Gly Tyr Thr Gly Thr Gly Tyr Val 
    15                  20                  25                  


Thr Asn Phe Ala Asp Ala Ala Ala Leu Leu Thr Phe Thr Val Asn Gly 
30                  35                  40                  45  


Leu Thr Ala Gly Ser Tyr Asp Leu Thr Leu Thr Tyr Ser Ala Gln Tyr 
                50                  55                  60      


Gly Asp Lys Phe Thr Thr Val Ser Val Asn Gly Ala Ser Gly Ile Glu 
            65                  70                  75          


Val Ala Ile Thr Asn Val Thr Thr Ala Thr Trp Thr Thr Ala Thr Ile 
        80                  85                  90              


Gly Thr Phe Thr Leu Thr Ala Gly Asp Asn Thr Val Ser Cys Ala Asp 
    95                  100                 105                 


Asp Trp Gly Trp Tyr Leu Ile Asp Ser Leu Thr Val Ile Pro Thr Pro 
110                 115                 120                 125 


Ala Lys Pro Ile Thr Ile Val Asp Val Ser Asn Gly Ala Thr Ala Gln 
                130                 135                 140     


Ala Glu Asp Gly Ile Leu Thr Gly Thr Thr Val Gly Thr Thr Thr Ala 
            145                 150                 155         


Gly Tyr Thr Gly Thr Gly Tyr Val Thr Gly Phe Thr Ala Thr Gly Thr 
        160                 165                 170             


Gln Val Thr Ile Asn Leu Ser Ser Thr Lys Gln Ala Leu Tyr Asp Val 
    175                 180                 185                 


Val Val Arg Tyr Ala Ala Ile Tyr Gly Gln Lys Tyr Thr Thr Met Gln 
190                 195                 200                 205 


Leu Asn Gly Val Gly Gly Ser Glu Ile Leu Leu Leu Asp Thr Thr Thr 
                210                 215                 220     


Ala Thr Ser Pro Trp Ala Asn Ala Thr Ala Gly Gln Val Leu Leu Ala 
            225                 230                 235         


Ser Gly Asn Asn Thr Leu Thr Phe Met Asn Asp Trp Gly Trp Tyr Phe 
        240                 245                 250             


Ile Asp Ala Val Tyr Val Thr Pro Ser Pro Ala Pro Ala Pro His Lys 
    255                 260                 265                 


Val Thr Asn Ala Leu Val Asp Ala Lys Ala Leu Ala Ser Thr His Ala 
270                 275                 280                 285 


Leu Phe Asn Thr Leu Leu Ala Lys Tyr Gly Ser Gly Asp Ile Phe Ser 
                290                 295                 300     


Gly Gln Ala Asp Pro Thr Gly Val Thr Trp Ile Glu Ser Asn Leu Gly 
            305                 310                 315         


Thr Thr Lys Thr Pro Ala Ile Ile Gly Leu Asp Met Ile Glu Tyr Ser 
        320                 325                 330             


Pro Thr Arg Val Leu Tyr Gly Ser Thr Ser Thr Ala Val Glu Asp Ala 
    335                 340                 345                 


Ile Ala Phe Asp Lys Arg Gly Gly Met Val Ala Phe Gln Trp His Trp 
350                 355                 360                 365 


Asn Ala Pro Ala Asp Leu Ile Asn Asn Asp Thr Val Pro Trp Trp Lys 
                370                 375                 380     


Gly Phe Tyr Ser Tyr Gly Thr Thr Phe Asn Leu Thr Ala Ala Leu Ala 
            385                 390                 395         


Asn Pro Ser Gly Ser Asp Tyr Ala Leu Leu Ile Ser Asp Met Asp Ala 
        400                 405                 410             


Ile Ala Val Gln Leu Leu Arg Leu Gln Ala Ala Gly Val Pro Val Leu 
    415                 420                 425                 


Trp Arg Pro Leu His Glu Ala Asp Gly Thr Trp Phe Trp Trp Gly Asn 
430                 435                 440                 445 


Phe Gly Ala Ala Ser Cys Val Ser Leu Tyr Arg Ile Met Tyr Asp Arg 
                450                 455                 460     


Tyr Thr Asn Tyr His Gly Leu His Asn Leu Ile Trp Val Trp Asn Ser 
            465                 470                 475         


Val Thr Pro Ser Trp Tyr Pro Gly Ala Asp Val Val Asp Ile Leu Gly 
        480                 485                 490             


Tyr Asp Ser Tyr Pro Ala Val Gly Asp His Gly Pro Val Ser Ser Gln 
    495                 500                 505                 


Tyr Asn Ala Leu Ile Thr Leu Gly Gly Asp Thr Lys Leu Val Thr Leu 
510                 515                 520                 525 


Pro Glu Val Gly Asn Ile Pro Asp Pro Ala Ile Leu Lys Leu Tyr His 
                530                 535                 540     


Ala Asp Trp Ser Tyr Phe Val Thr Trp Asn Gln Asp Tyr Ile Leu Thr 
            545                 550                 555         


Asp Thr Tyr Asn Pro Leu Ala Phe Lys Gln Gln Val Tyr Asn Asp Pro 
        560                 565                 570             


Thr Val Leu Lys Leu Thr Asp Leu Gly Asn Trp Lys Gly Ala Ala Thr 
    575                 580                 585                 


Ser Thr Ile Val Ser Ser Thr Ser Lys Val Ser Thr Thr Thr Ser Ser 
590                 595                 600                 605 


Leu Ile Thr Ser Thr Thr Lys Lys Thr Ser Ser Ser Thr Val Val Ser 
                610                 615                 620     


Thr Thr Ser Ser Thr Val Lys Thr Thr Ser Thr Thr Ser Lys Val Ser 
            625                 630                 635         


Ser Ser Thr Thr Lys Val Ser Ser Thr Thr Lys Val Thr Thr Thr Ser 
        640                 645                 650             


Thr Thr Thr Ser Ala Val Ala Thr Ala Thr Ala Gly His Trp Gly Gln 
    655                 660                 665                 


Cys Gly Gly Thr Gly Trp Thr Gly Pro Thr Val Cys Ala Ser Gly Phe 
670                 675                 680                 685 


Thr Cys Thr Ala Val Ser Pro Pro Tyr Tyr Tyr Gln Cys Leu 
                690                 695                 


