                         SEQUENCE LISTING

<110>  Monsanto Technology LLC
 
<120>  NOVEL INSECT INHIBITORY PROTEIN

<130>  MONS:394WO

<150>  US 62/199,024
<151>  2015-07-30

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  2814
<212>  DNA
<213>  Bacillus thuringiensis


<220>
<221>  misc_feature
<222>  (1)..(2814)
<223>  DNA sequence derived from Bt strain EG6657 encoding TIC5290.

<400>  1
atgcaaaata tcgtttcttc aaaaagcgaa caggcgacgg tcattggatt agttggtttt       60

tactttaaag atagtacatt taaagaattg atgtttatcc aggtgggcga aaaaagtaac      120

ttaatgaata aagcgagaat aaacacagat gcgcaacaaa ttcaatctat acgatggatg      180

gggaatttga agtctcccca aacgggagag tataggcttt ctacatcttc cgatgaaaat      240

gtaattctgc aaataaatgg cgaaacagtc attaatcaag ctagtataca aaaaaacctt      300

aaactagaag caaatcaggt atatgaaatc aagattgagt atcgtaatac atcgaataca      360

ttaccagatt tgcagttatt ctggtcaatg aacaatgcgc aaaaagaaca aatcccggag      420

aaatatatac tctctccaaa tttctctgaa aaagcaaatt cacttgccga aaaagaaaca      480

caaagtttct ttccaaatta taatttattt gatagacaac aagaaaatgg agaaaaacaa      540

tccatgtcta ctcctgtaga cacagataat gattgtattc cagatgaatg ggaagaaaag      600

ggatatacat ttagaaatca acaaattgtg ccatggaatg atgcatatag tgctgaaggc      660

tacaaaaaat atgtctcaaa tccttatcat gcacgaacag taaaagatcc ttatacagat      720

tttgaaaagg taacaggaca tatgccggca gcgacgaaat acgaagcaag agatccacta      780

gtagctgcgt atccatcggt tggagttgga atggaaaaac ttcatttttc taaaaatgat      840

actgtaactg agggaaatgc tgacacaaaa tctaaaacaa ccacaaaaac agatacgacc      900

acaaatactg tagagattgg tggatcgctc ggcttttcag ataaaggctt ttctttttca      960

atttcaccaa aatacacaca ttcttggagt agtagcacat ccgttgcaga tacggacagt     1020

acaacatggt cttcacaaat tggaattaac acggcagaaa gagcatattt aaatgcaaat     1080

gttcgttatt acaatggtgg aacggcacct atttatgatc taaaaccgac aacaaatttt     1140

gtcttccaaa attcaggtga ttccattact acgataacgg ctggacctaa tcaaattgga     1200

aatagtctag gtgcaggcga tacctatccg caaaaaggac aggccccgat ttcattagat     1260

aaagcaaatg aggctggtac agtaaaaatt gcaatcaatg ctgaacaatt agataaaata     1320

caagctggta cagaaatatt aaatatagaa actacgcaaa atagaggaca atatggaatt     1380

ttagatgaaa aaggtcaagt aattccaggt ggagaatggg atccgattcg aacaaatatt     1440

gatgcggtct ctggatcact cacattaaat cttggtacag ggaaagatag tctcgaacga     1500

agagtagctg caaaaaatat gaatgatcca gaagataaaa cacctgaaat tacaatcaaa     1560

gaagcaatca aaaaagcgtt taatgcacaa gaaaaagatg gtagattata ctatacggat     1620

caaggcgaaa aagatatatt tatcgatgaa ccttctatta atttaatcac agatgaaaat     1680

acaaaaaaag aaattgagcg ccaattaaat caaatgccag gtaaaacagt atatgatgta     1740

aaatggaaac gcgggatgaa gatcacactt catgtaccaa taaagtacta tgatttcgaa     1800

acctccgaaa atctatggta ttatacatac caagaaagcg gaggatatac gggtaaaaaa     1860

cgaggaagaa ttggtacaga tgggcatggg actgcgatgt caaatccaca attaaaaccg     1920

tatacaagtt atacggtgcg tgcatacgta cgaacagcat caacaacggg tagtaatgaa     1980

gttgtatttt atgcagataa tagctccggg aatggacaag gtgcaaaagt aagtggaaaa     2040

gtcacaggtg gtaaatggaa aatagcggaa ttttctttta atacttttaa caacccagag     2100

tattttaaaa taatcggttt gaaaaataac gggaatgcta atctccattt tgatgatgta     2160

tctgtaatag agtggaaaac aaatgaaaat cttcaaaaaa aacatatatt tgaaaaatgg     2220

agttttggtt caaatgatga gatggtgata ggtgcaacgt ttactcgtgt tccaagttcg     2280

aagattcgat accaatggaa aataaatggt aggttgggaa gtataatacc tgcaccgcca     2340

ttagacgcta atggtaaaag aactgtaacc tatggatcaa ttactgctat tactcccatg     2400

gaattatatg ctgtagatga aaaaaatgac aacctaaaag taaaagtagc tgaactcggc     2460

gagagtgaga ttgaaaaagt aatgatagat gcacataaat tttccgggtg gtggtattta     2520

tctgaaaacc caaacctgta tagtggtctt agtttataca aattacctga tatattttat     2580

aataacgtat cttcttataa aattcgagtg aatggaaaaa aagttcaaac agtctcaaaa     2640

ccaagcccat ttctttttca gataacgttt aatctaaaaa atcctaatgg tggcacttat     2700

cctactaaag atgcatcagt cgaattatgg gctacagtag gtggaaaaga tttaaaggtg     2760

ttgcataagt ggattcaaaa aagcgatgtt atgtacagtc agactaataa ttaa           2814


<210>  2
<211>  937
<212>  PRT
<213>  Bacillus thuringiensis


<220>
<221>  MISC_FEATURE
<222>  (1)..(937)
<223>  Amino acid sequence of TIC5290.

<400>  2

Met Gln Asn Ile Val Ser Ser Lys Ser Glu Gln Ala Thr Val Ile Gly 
1               5                   10                  15      


Leu Val Gly Phe Tyr Phe Lys Asp Ser Thr Phe Lys Glu Leu Met Phe 
            20                  25                  30          


Ile Gln Val Gly Glu Lys Ser Asn Leu Met Asn Lys Ala Arg Ile Asn 
        35                  40                  45              


Thr Asp Ala Gln Gln Ile Gln Ser Ile Arg Trp Met Gly Asn Leu Lys 
    50                  55                  60                  


Ser Pro Gln Thr Gly Glu Tyr Arg Leu Ser Thr Ser Ser Asp Glu Asn 
65                  70                  75                  80  


Val Ile Leu Gln Ile Asn Gly Glu Thr Val Ile Asn Gln Ala Ser Ile 
                85                  90                  95      


Gln Lys Asn Leu Lys Leu Glu Ala Asn Gln Val Tyr Glu Ile Lys Ile 
            100                 105                 110         


Glu Tyr Arg Asn Thr Ser Asn Thr Leu Pro Asp Leu Gln Leu Phe Trp 
        115                 120                 125             


Ser Met Asn Asn Ala Gln Lys Glu Gln Ile Pro Glu Lys Tyr Ile Leu 
    130                 135                 140                 


Ser Pro Asn Phe Ser Glu Lys Ala Asn Ser Leu Ala Glu Lys Glu Thr 
145                 150                 155                 160 


Gln Ser Phe Phe Pro Asn Tyr Asn Leu Phe Asp Arg Gln Gln Glu Asn 
                165                 170                 175     


Gly Glu Lys Gln Ser Met Ser Thr Pro Val Asp Thr Asp Asn Asp Cys 
            180                 185                 190         


Ile Pro Asp Glu Trp Glu Glu Lys Gly Tyr Thr Phe Arg Asn Gln Gln 
        195                 200                 205             


Ile Val Pro Trp Asn Asp Ala Tyr Ser Ala Glu Gly Tyr Lys Lys Tyr 
    210                 215                 220                 


Val Ser Asn Pro Tyr His Ala Arg Thr Val Lys Asp Pro Tyr Thr Asp 
225                 230                 235                 240 


Phe Glu Lys Val Thr Gly His Met Pro Ala Ala Thr Lys Tyr Glu Ala 
                245                 250                 255     


Arg Asp Pro Leu Val Ala Ala Tyr Pro Ser Val Gly Val Gly Met Glu 
            260                 265                 270         


Lys Leu His Phe Ser Lys Asn Asp Thr Val Thr Glu Gly Asn Ala Asp 
        275                 280                 285             


Thr Lys Ser Lys Thr Thr Thr Lys Thr Asp Thr Thr Thr Asn Thr Val 
    290                 295                 300                 


Glu Ile Gly Gly Ser Leu Gly Phe Ser Asp Lys Gly Phe Ser Phe Ser 
305                 310                 315                 320 


Ile Ser Pro Lys Tyr Thr His Ser Trp Ser Ser Ser Thr Ser Val Ala 
                325                 330                 335     


Asp Thr Asp Ser Thr Thr Trp Ser Ser Gln Ile Gly Ile Asn Thr Ala 
            340                 345                 350         


Glu Arg Ala Tyr Leu Asn Ala Asn Val Arg Tyr Tyr Asn Gly Gly Thr 
        355                 360                 365             


Ala Pro Ile Tyr Asp Leu Lys Pro Thr Thr Asn Phe Val Phe Gln Asn 
    370                 375                 380                 


Ser Gly Asp Ser Ile Thr Thr Ile Thr Ala Gly Pro Asn Gln Ile Gly 
385                 390                 395                 400 


Asn Ser Leu Gly Ala Gly Asp Thr Tyr Pro Gln Lys Gly Gln Ala Pro 
                405                 410                 415     


Ile Ser Leu Asp Lys Ala Asn Glu Ala Gly Thr Val Lys Ile Ala Ile 
            420                 425                 430         


Asn Ala Glu Gln Leu Asp Lys Ile Gln Ala Gly Thr Glu Ile Leu Asn 
        435                 440                 445             


Ile Glu Thr Thr Gln Asn Arg Gly Gln Tyr Gly Ile Leu Asp Glu Lys 
    450                 455                 460                 


Gly Gln Val Ile Pro Gly Gly Glu Trp Asp Pro Ile Arg Thr Asn Ile 
465                 470                 475                 480 


Asp Ala Val Ser Gly Ser Leu Thr Leu Asn Leu Gly Thr Gly Lys Asp 
                485                 490                 495     


Ser Leu Glu Arg Arg Val Ala Ala Lys Asn Met Asn Asp Pro Glu Asp 
            500                 505                 510         


Lys Thr Pro Glu Ile Thr Ile Lys Glu Ala Ile Lys Lys Ala Phe Asn 
        515                 520                 525             


Ala Gln Glu Lys Asp Gly Arg Leu Tyr Tyr Thr Asp Gln Gly Glu Lys 
    530                 535                 540                 


Asp Ile Phe Ile Asp Glu Pro Ser Ile Asn Leu Ile Thr Asp Glu Asn 
545                 550                 555                 560 


Thr Lys Lys Glu Ile Glu Arg Gln Leu Asn Gln Met Pro Gly Lys Thr 
                565                 570                 575     


Val Tyr Asp Val Lys Trp Lys Arg Gly Met Lys Ile Thr Leu His Val 
            580                 585                 590         


Pro Ile Lys Tyr Tyr Asp Phe Glu Thr Ser Glu Asn Leu Trp Tyr Tyr 
        595                 600                 605             


Thr Tyr Gln Glu Ser Gly Gly Tyr Thr Gly Lys Lys Arg Gly Arg Ile 
    610                 615                 620                 


Gly Thr Asp Gly His Gly Thr Ala Met Ser Asn Pro Gln Leu Lys Pro 
625                 630                 635                 640 


Tyr Thr Ser Tyr Thr Val Arg Ala Tyr Val Arg Thr Ala Ser Thr Thr 
                645                 650                 655     


Gly Ser Asn Glu Val Val Phe Tyr Ala Asp Asn Ser Ser Gly Asn Gly 
            660                 665                 670         


Gln Gly Ala Lys Val Ser Gly Lys Val Thr Gly Gly Lys Trp Lys Ile 
        675                 680                 685             


Ala Glu Phe Ser Phe Asn Thr Phe Asn Asn Pro Glu Tyr Phe Lys Ile 
    690                 695                 700                 


Ile Gly Leu Lys Asn Asn Gly Asn Ala Asn Leu His Phe Asp Asp Val 
705                 710                 715                 720 


Ser Val Ile Glu Trp Lys Thr Asn Glu Asn Leu Gln Lys Lys His Ile 
                725                 730                 735     


Phe Glu Lys Trp Ser Phe Gly Ser Asn Asp Glu Met Val Ile Gly Ala 
            740                 745                 750         


Thr Phe Thr Arg Val Pro Ser Ser Lys Ile Arg Tyr Gln Trp Lys Ile 
        755                 760                 765             


Asn Gly Arg Leu Gly Ser Ile Ile Pro Ala Pro Pro Leu Asp Ala Asn 
    770                 775                 780                 


Gly Lys Arg Thr Val Thr Tyr Gly Ser Ile Thr Ala Ile Thr Pro Met 
785                 790                 795                 800 


Glu Leu Tyr Ala Val Asp Glu Lys Asn Asp Asn Leu Lys Val Lys Val 
                805                 810                 815     


Ala Glu Leu Gly Glu Ser Glu Ile Glu Lys Val Met Ile Asp Ala His 
            820                 825                 830         


Lys Phe Ser Gly Trp Trp Tyr Leu Ser Glu Asn Pro Asn Leu Tyr Ser 
        835                 840                 845             


Gly Leu Ser Leu Tyr Lys Leu Pro Asp Ile Phe Tyr Asn Asn Val Ser 
    850                 855                 860                 


Ser Tyr Lys Ile Arg Val Asn Gly Lys Lys Val Gln Thr Val Ser Lys 
865                 870                 875                 880 


Pro Ser Pro Phe Leu Phe Gln Ile Thr Phe Asn Leu Lys Asn Pro Asn 
                885                 890                 895     


Gly Gly Thr Tyr Pro Thr Lys Asp Ala Ser Val Glu Leu Trp Ala Thr 
            900                 905                 910         


Val Gly Gly Lys Asp Leu Lys Val Leu His Lys Trp Ile Gln Lys Ser 
        915                 920                 925             


Asp Val Met Tyr Ser Gln Thr Asn Asn 
    930                 935         


<210>  3
<211>  2814
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic coding sequence encoding TIC5290 designed for 
       expression in plants.

<400>  3
atgcagaaca ttgtctcctc caagagtgag caagcgacgg tcatcggcct ggtcggcttc       60

tacttcaagg actcaacctt caaggagctc atgttcatcc aggtgggcga gaagtccaac      120

ctcatgaaca aggctcgcat caacacggac gcccagcaga tccagtccat tcggtggatg      180

ggcaacctga agagcccgca gaccggcgag taccgcctct ccacatcctc cgacgagaac      240

gtaatcctcc agatcaatgg cgagacggtc atcaaccagg cgagcatcca gaagaacctc      300

aaactagagg caaaccaggt ctacgagatt aagatcgagt acaggaacac ctccaacacc      360

ctgccggacc tacagctctt ctggtcgatg aacaacgcgc agaaggaaca gatcccggag      420

aagtacatac tgagcccgaa tttcagcgag aaggcgaact ctctcgcgga gaaggagacc      480

cagagcttct tcccgaacta caacctcttc gaccgccagc aagagaacgg cgagaagcag      540

tcgatgtcca cgccggtgga caccgacaac gactgcatcc ctgatgaatg ggaagagaaa      600

ggatacacct tccgtaacca gcagatcgtg ccgtggaacg acgcctacag tgcagaaggc      660

tacaagaagt acgtgagcaa cccttaccac gcccgtacgg tcaaagaccc gtacaccgac      720

ttcgagaagg tgactgggca catgcccgct gctacgaagt atgaggcgcg cgatcctcta      780

gtggccgcct atccctccgt tggcgtcgga atggagaagc tccacttcag caagaacgat      840

acggtgacgg agggcaacgc ggatacaaag agcaagacta caactaagac cgacaccacg      900

accaacaccg tcgagatcgg cggcagcctg ggcttcagcg acaagggctt cagtttctca      960

atctcaccaa agtacaccca cagctggtcg tcgtccacaa gtgtggccga caccgactct     1020

actacctgga gttcgcagat agggatcaac actgccgaga gggcgtatct caacgcgaac     1080

gtgcgctatt acaatggtgg caccgcgccc atctacgacc tgaagccgac caccaacttc     1140

gtcttccaga actcaggcga cagcatcacc acgatcaccg ccggccctaa ccagatcggc     1200

aactcgctcg gtgccggcga cacctatccg cagaagggcc aggctcctat ctccctagac     1260

aaggccaacg aggcgggcac cgtgaagata gcgatcaacg ccgagcagct ggacaagatc     1320

caggcgggca cggagattct caacatcgag actacgcaga accgcggcca gtacggtatc     1380

ctcgacgaga aaggccaggt gatacccgga ggcgagtggg acccgatccg gacaaacatt     1440

gacgctgtca gtgggagcct tactcttaac ctcggcacgg gcaaggatag cctcgagcgc     1500

cgggtcgcgg cgaagaacat gaacgatccg gaggacaaga ctccggagat caccatcaag     1560

gaggccatca agaaggcgtt taacgctcag gagaaggacg gcagactgta ctacacggac     1620

cagggtgaga aggacatctt cattgatgag ccttccatca acctcatcac ggacgagaac     1680

acgaagaaag aaatcgagcg ccagctgaac cagatgcccg gcaagacggt gtacgacgtg     1740

aagtggaagc gcggcatgaa gatcacgcta cacgtcccga tcaagtacta cgacttcgag     1800

acctcagaga acctgtggta ctacacctac caagaatccg gaggctacac cggcaagaag     1860

cgcgggcgga tcggcactga cgggcacggc acggcgatgt caaacccgca gctgaagcca     1920

tacacctcct acactgtgcg ggcgtacgtg cgcaccgcca gcaccactgg gagcaacgag     1980

gtcgtcttct acgctgacaa cagctccggc aacgggcaag gcgcgaaggt ttccgggaag     2040

gtgaccggcg ggaagtggaa gatagcggag ttctccttca acacgttcaa taaccctgaa     2100

tacttcaaga tcatcggcct gaagaataac gggaacgcca acctgcactt cgacgatgtc     2160

tccgtgatcg agtggaagac caacgagaac ctgcagaaga aacacatctt tgagaagtgg     2220

tccttcggct ccaacgacga gatggtgatc ggtgccacgt tcactcgcgt gccgagtagc     2280

aagatccgat accagtggaa gatcaacggc cgcctcggta gcatcatccc tgcgccgcct     2340

ctggacgcca acgggaagcg gacggtgacg tacggcagca tcactgcgat cacgcctatg     2400

gagctctacg ccgttgacga gaagaacgac aacctcaaag tgaaagtcgc tgagcttggc     2460

gagtccgaga tcgagaaagt tatgatcgac gcccacaaat tctcaggctg gtggtatctc     2520

tcagagaatc caaacctgta ctccggcctc agcctgtaca agctgcccga catcttctac     2580

aacaacgtgt cgagctacaa gatccgcgtg aacggcaaga aggtccagac cgtcagcaag     2640

ccgagcccgt tcttgttcca gattacgttt aatctcaaga accctaacgg cgggacctac     2700

ccgacgaaag atgctagcgt agagctctgg gcgacggtcg gcgggaagga cctgaaggtg     2760

ctacacaagt ggattcagaa gtcggatgtc atgtacagcc agaccaacaa ctga           2814


