                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Camps, Manel
 
<120>  System for continuous mutagenesis in vivo to facilitate directed 
       evolution

<130>  SC2015_656_PCT

<140>  PCT/US 16/43894
<141>  2016-07-25

<150>  US 62/196,527
<151>  2015-07-24

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EP-Pol I (E. coli Pol I with I709N, A759R, D424A)

<400>  1

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  2
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E

<400>  2

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  3
<211>  68
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr 
65              


<210>  4
<211>  69
<212>  PRT
<213>  Deinococcus radiodurans

<400>  4

Asp Ala Ser Pro Asp Pro Ser Lys Pro Asp Ala Leu Val Leu Ile Asp 
1               5                   10                  15      


Gly His Ala Leu Ala Phe Arg Ser Tyr Phe Ala Leu Pro Pro Leu Asn 
            20                  25                  30          


Asn Ser Lys Gly Glu Met Thr Asp Ala Ile Val Gly Phe Met Lys Leu 
        35                  40                  45              


Leu Leu Arg Leu Ala Arg Gln Ser Asn Gln Val Ile Val Val Phe Asp 
    50                  55                  60                  


Pro Pro Val Lys Thr 
65                  


<210>  5
<211>  70
<212>  PRT
<213>  Thermus aquaticus

<400>  5

Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val Asp 
1               5                   10                  15      


Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly Leu Thr 
            20                  25                  30          


Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser 
        35                  40                  45              


Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val Val Phe 
    50                  55                  60                  


Asp Ala Lys Ala Pro Ser 
65                  70  


<210>  6
<211>  70
<212>  PRT
<213>  Thermus flavus

<400>  6

Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val Asp 
1               5                   10                  15      


Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr 
            20                  25                  30          


Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser 
        35                  40                  45              


Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Val Val Val Val Val Phe 
    50                  55                  60                  


Asp Ala Lys Ala Pro Ser 
65                  70  


<210>  7
<211>  69
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  7

Met Asp Lys Lys Lys Leu Leu Leu Ile Asp Gly Ser Ser Val Ala Phe 
1               5                   10                  15      


Arg Ala Phe Phe Ala Leu Tyr Gln Gln Leu Asp Arg Phe Lys Asn Ala 
            20                  25                  30          


Ala Gly Leu His Thr Asn Ala Ile Tyr Gly Phe Gln Leu Met Leu Ser 
        35                  40                  45              


His Leu Leu Glu Arg Val Glu Pro Ser His Ile Leu Val Ala Phe Asp 
    50                  55                  60                  


Ala Gly Lys Thr Thr 
65                  


<210>  8
<211>  64
<212>  PRT
<213>  Geobacillus sp.

<400>  8

Met Lys Lys Lys Leu Val Leu Ile Asp Gly Ser Ser Val Ala Tyr Arg 
1               5                   10                  15      


Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              


Glu Glu Pro Thr His Met Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  


<210>  9
<211>  74
<212>  PRT
<213>  Bacteriophage T4

<400>  9

Glu Met Met Leu Asp Glu Asp Tyr Lys Glu Gly Ile Cys Leu Ile Asp 
1               5                   10                  15      


Phe Ser Gln Ile Ala Leu Ser Thr Ala Leu Val Asn Phe Pro Asp Lys 
            20                  25                  30          


Glu Lys Ile Asn Leu Ser Met Val Arg His Leu Ile Leu Asn Ser Ile 
        35                  40                  45              


Lys Phe Asn Val Lys Lys Ala Arg Thr Leu Gly Tyr Thr Lys Ile Val 
    50                  55                  60                  


Leu Cys Ile Asp Asn Ala Lys Ser Gly Tyr 
65                  70                  


<210>  10
<211>  63
<212>  PRT
<213>  Bacteriophage T5

<400>  10

Glu Glu Glu Ala Glu Met Ala Ser Arg Arg Asn Leu Met Ile Val Asp 
1               5                   10                  15      


Gly Thr Asn Leu Gly Phe Arg Phe Lys His Asn Asn Ser Lys Lys Pro 
            20                  25                  30          


Phe Ala Ser Ser Tyr Val Ser Thr Ile Gln Ser Leu Ala Lys Ser Tyr 
        35                  40                  45              


Ser Ala Arg Thr Thr Ile Val Leu Gly Asp Lys Gly Lys Ser Val 
    50                  55                  60              


<210>  11
<211>  80
<212>  PRT
<213>  Bacteriophage T3

<400>  11

Tyr Glu Leu Arg Glu Gly Cys Asp Asp Lys Gly Ile Leu Val Met Asp 
1               5                   10                  15      


Gly Asp Trp Leu Val Phe Gln Ala Met Ser Ala Ala Glu Phe Asp Ala 
            20                  25                  30          


Ser Trp Glu Glu Glu Ile Trp His Arg Cys Cys Asp His Ala Lys Ala 
        35                  40                  45              


Arg Gln Ile Leu Glu Asp Ser Ile Lys Ser Tyr Glu Thr Arg Lys Lys 
    50                  55                  60                  


Ala Trp Val Gly Ala Pro Ile Val Leu Ala Phe Thr Asp Ser Val Asn 
65                  70                  75                  80  


<210>  12
<211>  80
<212>  PRT
<213>  Bacteriophage T7

<400>  12

Tyr Glu Leu Arg Glu Gly Cys Asp Asp Lys Gly Ile Leu Val Met Asp 
1               5                   10                  15      


Gly Asp Trp Leu Val Phe Gln Ala Met Ser Ala Ala Glu Phe Asp Ala 
            20                  25                  30          


Ser Trp Glu Glu Glu Ile Trp His Arg Cys Cys Asp His Ala Lys Ala 
        35                  40                  45              


Arg Gln Ile Leu Glu Asp Ser Ile Lys Ser Tyr Glu Thr Arg Lys Lys 
    50                  55                  60                  


Ala Trp Ala Gly Ala Pro Ile Val Leu Ala Phe Thr Asp Ser Val Asn 
65                  70                  75                  80  


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence: motif A

<400>  13

Leu Leu Leu Val Asp Gly Phe 
1               5           


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence: motif B

<400>  14

Ile Val Leu Phe Asp 
1               5   


<210>  15
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K54E-LF Pol I (E. coli Pol I with K54E, I709N, A759R, D424A)

<400>  15

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  16
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with I709N

<400>  16

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  17
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with A759R

<400>  17

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  18
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with D424A

<400>  18

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  19
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, I709N

<400>  19

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  20
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, A759R

<400>  20

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  21
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, D424A

<400>  21

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  22
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with I709N, A759R

<400>  22

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  23
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with I709N, D424A

<400>  23

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  24
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with A759R, D424A

<400>  24

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  25
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, I709N, A759R

<400>  25

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  26
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, I709N, D424A

<400>  26

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Asn Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


<210>  27
<211>  928
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli Pol I with K54E, A759R, D424A

<400>  27

Met Val Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser 
1               5                   10                  15      


Tyr Leu Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala 
            20                  25                  30          


Gly Glu Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser 
        35                  40                  45              


Leu Ile Met Gln Tyr Glu Pro Thr His Ala Ala Val Val Phe Asp Ala 
    50                  55                  60                  


Lys Gly Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His 
65                  70                  75                  80  


Arg Pro Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His 
                85                  90                  95      


Ala Met Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val 
            100                 105                 110         


Glu Ala Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala 
        115                 120                 125             


Gly Arg Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu 
    130                 135                 140                 


Val Thr Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu 
145                 150                 155                 160 


Gly Pro Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile 
                165                 170                 175     


Ile Asp Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu 
        195                 200                 205             


Gly Gly Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu 
    210                 215                 220                 


Ser Phe Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys 
225                 230                 235                 240 


Glu Val Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val 
                245                 250                 255     


Glu Leu Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala 
            260                 265                 270         


Glu Glu Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr 
        275                 280                 285             


Ala Asp Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro 
    290                 295                 300                 


Ala Ala Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val 
305                 310                 315                 320 


Thr Ala Thr Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu 
                325                 330                 335     


Glu Thr Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe 
            340                 345                 350         


Ala Phe Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu 
        355                 360                 365             


Val Gly Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro 
    370                 375                 380                 


Val Ala His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg 
385                 390                 395                 400 


Ala Leu Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys 
                405                 410                 415     


Val Gly Gln Asn Leu Lys Tyr Ala Arg Gly Ile Leu Ala Asn Tyr Gly 
            420                 425                 430         


Ile Glu Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile 
        435                 440                 445             


Leu Asn Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg 
    450                 455                 460                 


Trp Leu Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly 
465                 470                 475                 480 


Lys Asn Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg 
                485                 490                 495     


Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met 
            500                 505                 510         


Trp Pro Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn 
        515                 520                 525             


Ile Glu Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly 
    530                 535                 540                 


Val Lys Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr 
545                 550                 555                 560 


Leu Arg Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu 
                565                 570                 575     


Glu Phe Asn Leu Ser Ser Thr Lys Glu Leu Gln Thr Ile Leu Phe Glu 
            580                 585                 590         


Lys Gln Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser 
        595                 600                 605             


Thr Ser Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro 
    610                 615                 620                 


Lys Val Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr 
625                 630                 635                 640 


Thr Asp Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His 
                645                 650                 655     


Thr Ser Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr 
            660                 665                 670         


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg 
        675                 680                 685             


Ile Arg Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala 
    690                 695                 700                 


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Met Ala Tyr Leu Ser Arg Asp 
705                 710                 715                 720 


Lys Gly Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala 
                725                 730                 735     


Thr Ala Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu 
            740                 745                 750         


Gln Arg Arg Ser Ala Lys Arg Ile Asn Phe Gly Leu Ile Tyr Gly Met 
        755                 760                 765             


Ser Ala Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala 
    770                 775                 780                 


Gln Lys Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu 
785                 790                 795                 800 


Tyr Met Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu 
                805                 810                 815     


Thr Leu Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn 
            820                 825                 830         


Gly Ala Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met 
        835                 840                 845             


Gln Gly Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp 
    850                 855                 860                 


Ala Trp Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val 
865                 870                 875                 880 


His Asp Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val 
                885                 890                 895     


Ala Lys Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val 
            900                 905                 910         


Pro Leu Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        915                 920                 925             


