                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DES SCIENCES APPLIQUEES 
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
       INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
 
<120>  NEW POLYPEPTIDE HAVING FERREDOXIN-NADP+REDUCTASE ACTIVITY, 
       POLYNUCLEOTIDE ENCODING THE SAME AND USES THEREOF

<130>  B370797D31747

<150>  EP 15306225.2
<151>  2015-07-27

<160>  95    

<170>  PatentIn version 3.5

<210>  1
<211>  411
<212>  PRT
<213>  C. acetobutylicum

<400>  1

Met Asp Asn Pro Asn Leu Leu Ser Glu Glu Ala Asn Arg Cys Leu Leu 
1               5                   10                  15      


Cys Lys Asn Pro Arg Cys Lys Ala Asn Cys Pro Ile Asn Thr Pro Ile 
            20                  25                  30          


Pro Glu Ile Ile Ser Leu Tyr Lys Glu Gly Lys Ile Met Glu Ala Gly 
        35                  40                  45              


Glu Ile Leu Phe Asn Asn Asn Pro Leu Ser Val Ile Cys Ser Leu Val 
    50                  55                  60                  


Cys Ile His Glu Asp Gln Cys Lys Gly Asn Cys Val Arg Gly Ile Lys 
65                  70                  75                  80  


Ser Glu Pro Ile Lys Phe His Glu Ile Glu Glu Glu Ile Ser Glu Lys 
                85                  90                  95      


Tyr Leu Lys Glu Ala Lys Leu Lys Asn Val Gln Lys Asp Lys Asp Arg 
            100                 105                 110         


Ile Ala Ile Val Gly Gly Gly Pro Ala Gly Ile Thr Val Ala Phe Val 
        115                 120                 125             


Leu Ala Asn Lys Gly Tyr Asn Val Thr Ile Phe Glu Ala His Asp Lys 
    130                 135                 140                 


Ile Gly Gly Val Leu Arg Tyr Gly Ile Pro Glu Tyr Arg Leu Thr Lys 
145                 150                 155                 160 


Lys Leu Val Asp Lys Leu Glu Glu Arg Leu Ile Glu Val Gly Val Lys 
                165                 170                 175     


Ile Arg Pro Asn Thr Val Ile Gly Pro Val Ile Ser Leu Asp Arg Leu 
            180                 185                 190         


Leu Glu Asp Ser Tyr Lys Ala Val Phe Ile Gly Thr Gly Val Trp Asn 
        195                 200                 205             


Pro Lys Thr Leu Asp Val Lys Gly Glu Thr Leu Gly Asn Val His Phe 
    210                 215                 220                 


Ala Ile Asp Tyr Leu Lys Ser Pro Glu Ser Tyr Arg Leu Gly Lys Lys 
225                 230                 235                 240 


Val Ala Val Ile Gly Ala Gly Asn Val Ala Met Asp Ala Ala Arg Thr 
                245                 250                 255     


Ala Lys Arg Asn Gly Ala Glu Val Thr Ile Leu Tyr Arg Lys Ser Phe 
            260                 265                 270         


Asn Glu Met Pro Ala Ser Lys Gln Glu Ile Arg Glu Thr Lys Glu Asp 
        275                 280                 285             


Gly Val Glu Phe Lys Leu Phe Arg Ala Pro Ile Glu Ile Thr Glu Glu 
    290                 295                 300                 


Gly Ile Lys Val Ala Phe Thr Glu Asn Val Thr Asp Ala Glu Gly Lys 
305                 310                 315                 320 


Ile Arg Thr Lys Ile Ile Glu Gly Lys Glu Glu Phe Phe Glu Cys Asp 
                325                 330                 335     


Ser Val Val Val Ala Val Ser Gln Ala Pro Lys Asp Asn Ile Val Ser 
            340                 345                 350         


Asn Thr Thr Gly Leu Asp Thr Lys Trp Gly Leu Ile Val Thr Asp Glu 
        355                 360                 365             


Lys Gly Asn Thr Thr Lys Lys Gly Thr Phe Ala Cys Gly Asp Val Val 
    370                 375                 380                 


Thr Gly Ala Lys Thr Val Val Glu Ala Ala Ala Gln Ala Lys Val Val 
385                 390                 395                 400 


Ala Glu Thr Ile Asp Glu Tyr Cys Lys Asn Asn 
                405                 410     


<210>  2
<211>  1236
<212>  DNA
<213>  C. acetobutylicum

<400>  2
atggataacc ctaatttatt gtcagaagag gcaaacagat gcctactatg caaaaaccct       60

agatgtaaag caaattgccc tattaataca cccataccag aaattataag cctttataaa      120

gaaggaaaaa ttatggaagc aggagaaatt ttatttaata acaatcctct ttcagtaata      180

tgctcattgg tctgtattca tgaggatcaa tgtaagggaa attgtgtaag aggaataaaa      240

agcgagccaa taaaatttca cgagatagaa gaagagatat cagagaagta cttaaaagag      300

gccaagctta agaatgttca gaaggacaag gatagaattg caatagttgg aggaggtcca      360

gcaggaatca cagttgcatt tgtacttgct aataaaggct ataatgttac catttttgag      420

gctcacgata aaataggagg agtacttaga tatggtattc cagagtatag attgactaag      480

aaattagtgg ataagcttga agaaagactt atagaggttg gagtgaaaat tagacctaat      540

actgttattg gaccagttat ttctttggac aggttacttg aagattcata taaggcagta      600

tttattggaa ctggagtatg gaatccaaaa accttagatg taaagggaga aactttagga      660

aatgttcatt ttgctattga ttatttaaaa tctcctgaaa gctatagatt aggaaaaaaa      720

gtagctgtaa taggagcagg taatgttgcc atggatgctg caagaactgc taagagaaat      780

ggtgctgagg taacaatact ttatagaaaa agctttaatg agatgccagc atcaaaacaa      840

gaaataagag aaactaaaga agatggagta gagtttaaat tatttagagc accaattgaa      900

ataacagaag agggtattaa agtagcattt acagaaaatg ttacagatgc agaaggaaaa      960

ataagaacta aaattattga aggaaaagaa gagttttttg agtgtgattc agtagttgtt     1020

gccgtaagtc aagcacctaa ggataatata gtatctaata caacaggctt agatactaaa     1080

tggggattaa tagtaacaga tgaaaaaggc aatacaacta aaaaaggaac ctttgcatgt     1140

ggagatgtag ttacaggagc aaaaactgta gttgaagctg cagcacaagc aaaagtagtt     1200

gcagaaacta ttgatgagta ttgcaagaat aattaa                               1236


<210>  3
<211>  53
<212>  DNA
<213>  artificial

<220>
<223>  primer : 408/409s-IBS consisting of 53 bases

<400>  3
aaaaaagctt ataattatcc ttaggctaca atgttgtgcg cccagatagg gtg              53


<210>  4
<211>  60
<212>  DNA
<213>  artificial

<220>
<223>  primer:408/409s-EBS1d consisting of 60 bases

<400>  4
cagattgtac aaatgtggtg ataacagata agtcaatgtt actaacttac ctttctttgt       60


<210>  5
<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  primer:408/409s-EBS2 consisting of 49 bases

<400>  5
tgaacgcaag tttctaattt cgatttagcc tcgatagagg aaagtgtct                   49


<210>  6
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 886263 to 886287

<400>  6
cgagccaata aaatttcacg agata                                             25


<210>  7
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 886512 to 886541

<400>  7
ccaacctcta taagtctttc ttcaagctta                                        30


<210>  8
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer: Ocac0764f

<400>  8
aggatccatc aaaatttagg aggttagtta                                        30


<210>  9
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  primer: Ocac0764r

<400>  9
ggcgccttaa ttattcttgc aatactcatc aatagtttc                              39


<210>  10
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (1439878-1439958) of 
       the ldhA gene (sequence  1439878 to 1440867), and a region for 
       the amplification of the chloramphenicol resistance cassette of 
       the pKD3 plasmid

<400>  10
ttaaaccagt tcgttcgggc aggtttcgcc tttttccaga ttgcttaagt tttgcagcgt       60

agtctgagaa atactggtca gcatatgaat atcctcctta g                          101


<210>  11
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (1440786-1440865) of 
       the ldhA gene (sequence 1439878 to 1440867), and a region for the
       amplification of the chloramphenicol resistance cassette carried 
       by the pKD3 plasmid.

<400>  11
gaaactcgcc gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga       60

gtcctttggc tttgagctgg tgtaggctgg agctgcttcg                            100


<210>  12
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli  sequence 1439724 to 1439743

<400>  12
gccatcagca ggcttagcgc                                                   20


<210>  13
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1441007 to 1441029

<400>  13
gggtattgtg gcatgtttaa ccg                                               23


<210>  14
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (952236 -952315) of 
       the pflB gene (sequence 950495-952777), and a region (upper-case 
       letters) for the amplification of the chloramphenicol resistance 
       cassette of the pKD3 plasmid

<400>  14
ccggacatcc tgcgttgccg taaatctggt gttctgaccg gtctgccaga tgcatatggc       60

cgtggccgta tcatcggtga catatgaata tcctccttag                            100


<210>  15
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (949470-949549) 
       located upstream the pflA gene (sequence 949563-950303), and a 
       region for the amplification of the chloramphenicol resistance 
       cassette carried by the pKD3 plasmid.

<400>  15
gatgcactat aagatgtgtt aaaaacgctg tagcagaatg aagcgcggaa taaaaaagcg       60

gcaactcaat aaagttgccg tgtaggctgg agctgcttcg                            100


<210>  16
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli the sequence 948462 to 948491

<400>  16
agacattaaa aatatacgtg cagctacccg                                        30


<210>  17
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 953660  to 953689

<400>  17
gtgaaagctg acaacccttt tgatctttta                                        30


<210>  18
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (2411494-2411573) of 
       the ackA gene (sequence  2411492 to 2412694), and a region for 
       the amplification of the chloramphenicol resistance cassette of 
       the pKD3 plasmid

<400>  18
gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat ttgccatcat       60

cgatgcagta aatggtgaag tgtaggctgg agctgcttcg                            100


<210>  19
<211>  97
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (2414906-2414830) of 
       the pta gene (sequence 2412769 to 2414913), and a region for the 
       amplification of the chloramphenicol resistance cassette carried 
       by the pKD3 plasmid.

<400>  19
tgctgtgcag actgaatcgc agtcagcgcg atggtgtaga cgatatcgtc aaccagtgcg       60

ccacgggaca ggtcgttcat atgaatatcc tccttag                                97


<210>  20
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 2410902 to 2410921

<400>  20
gcatgggtaa acttaaggcg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 2415147 to 2415166

<400>  21
taatcaccaa cgtatcgggc                                                   20


<210>  22
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (2660151-2660200) 
       corresponding to the upstream chromosomal seq of the iscR gene 
       (seq 2659665 to 2660153), and a region for the amplification of 
       the kanamycin resistance cassette carried by the pKD13 plasmid

<400>  22
tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg attccgggga       60

tccgtcgacc                                                              70


<210>  23
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (2659636-2659685) 
       corresponding to the downstream chromosomal sequence of the iscR 
       gene (sequence 2659665 to 2660153), and a region for the 
       amplification of the kanamycin resistance cassette of the pKD13 
       

<400>  23
cactccggcc tgattctgaa ttctttttat taagcgcgta acttaacgtc tgtaggctgg       60

agctgcttcg                                                              70


<210>  24
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 2660325 to 2660343

<400>  24
cgccgcatcc gacaacagg                                                    19


<210>  25
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E; coli sequence 2659253 to 2659275

<400>  25
tgctggtgat gatgtgcttg cct                                               23


<210>  26
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer : a region homologous to the seq (4380339-4380388) 
       corresponding to the upstream chromosomal seq of the frdA gene 
       (seq 4378533 to 4380341), and a region for the amplification of 
       the chloramphenicol resistance cassette of the plasmid pKD3

<400>  26
accctgaagt acgtggctgt gggataaaaa caatctggag gaatgtcgtg tgtaggctgg       60

agctgcttcg                                                              70


<210>  27
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (4377001-4377050) 
       corresponding to the downstream chromosomal seq of the frdD gene 
       (seq 4377030 to 4377389), and a region for the amplification of 
       the chloramphenicol resistance cassette carried by pKD3

<400>  27
aggcgggccg gatttacatt ggcgatgcgt tagattgtaa cgacaccaat catatgaata       60

tcctccttag                                                              70


<210>  28
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 4380779 to 4380800

<400>  28
ctggctcata caaggcgtct cc                                                22


<210>  29
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E coli sequence 4376610 to 4376632

<400>  29
tcccattcca ctgtttagcg gta                                               23


<210>  30
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (122970-123019) 
       corresponding to the upstream chromosomal seq of the aceE  gene 
       (seq 123017 to 125680),  and a region for the amplification of 
       the kanamycin resistance cassette carried by the pKD13 plasmid

<400>  30
acaggttcca gaaaactcaa cgttattaga tagataagga ataacccatg attccgggga       60

tccgtcgacc                                                              70


<210>  31
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (125660-125709) 
       corresponding to the downstream chromosomal seq of the aceE gene 
       (seq 123017 to 125680), and a region for the amplification of the
       kanamycin resistance cassette of the pKD13 plasmid

<400>  31
gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tgtaggctgg       60

agctgcttcg                                                              70


<210>  32
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 122806 to 122825

<400>  32
gagagccgcc gtgagcgttc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 125916 to 125935

<400>  33
ctgcaccgtc ggcggaatcg                                                   20


<210>  34
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (1026236-1026285) 
       corresponding to the upstream chromosomal seq of the mgsA gene 
       (seq 1025780 to 1026238), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  34
taagtgctta cagtaatctg taggaaagtt aactacggat gtacattatg tgtaggctgg       60

agctgcttcg                                                              70


<210>  35
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (1025751-1025800) 
       corresponding to the downstream chromosomal seq of the mgsA gene 
       (seq 1025780 to 1026238), and a region for the amplification of 
       the chloramphenicol resistance cassette carried by pKD3

<400>  35
aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag catatgaata       60

tcctccttag                                                              70


<210>  36
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1026715 to 1026734

<400>  36
cccagctcat caaccaggtc                                                   20


<210>  37
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1025559 to 1025580

<400>  37
ggagtcgatt atggaagagg cg                                                22


<210>  38
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (1297264 -1297344) 
       including the adhE gene initiation codon of the adhE gene 
       (sequence 1294669 to 1297344), and a region for the amplification
       of the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  38
atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag       60

cgtgaatatg ccagtttcac tcatatgaat atcctcctta g                          101


<210>  39
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (1294693-1294748) 
       corresponding to the downstream chromosomal seq of the adhE gene 
       (sequence1294669 to 1297344), and a region for the amplification 
       of the chloramphenicol resistance cassette carried by pKD3

<400>  39
ttaagcggat tttttcgctt ttttctcagc tttagccgga gcagcttctt tcttcgctgc       60

agtttcacct tctacataat tgtaggctgg agctgcttcg                            100


<210>  40
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 1294357 to 1294378

<400>  40
ggctcattgc accaccatcc ag                                                22


<210>  41
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 1297749 to 1297772

<400>  41
gaaaagacgc gctgacaata cgcc                                              24


<210>  42
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer Ome1847

<400>  42
ttcgtcacgg aatcgtcaga ac                                                22


<210>  43
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer Ome1850

<400>  43
cctgatttat accggcattt cgg                                               23


<210>  44
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence from 4340168 to 
       4340187

<400>  44
gccgattttg tcgtggtggc                                                   20


<210>  45
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the  E. coli sequence from 4344065 to 
       4344044

<400>  45
gccggttatc catcaggttc ac                                                22


<210>  46
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: Homologous to a region for the amplification of the 
       chloramphenicol resistance cassette of the pKD3 plasmid

<400>  46
tgtaggctgg agctgcttcg                                                   20


<210>  47
<211>  94
<212>  DNA
<213>  artificial

<220>
<223>  primer:a region homologous to the seq 2324141-2324131 of the atoB
       gene, a region homologous to the RBS01*2 consensus seq, a region 
       containing the artificial ptrc30 promoter, a region for the 
       amplification of the chloramphenicol resistance cassette of pKD3

<400>  47
caatttttca tttataacct ccttattcca cacagtatac gagccggatg attaatcgtc       60

aacagctcca tggtccatat gaatatcctc ctta                                   94


<210>  48
<211>  137
<212>  DNA
<213>  artificial

<220>
<223>  Primer: a region homologous to the seq 2324042-2324130 upstream 
       of the atoB gene, a region corresponding to the transcriptional 
       terminator T7T of the T7 phage and a region for the amplification
       of the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  48
gcatcactgc cctgctcttc tccggtgtca ttttcgtcat tggtttaacg ctgttctgac       60

ggcaccccta caaacagaag gaatataaac tggctcacct tcgggtgggc ctttctgctg      120

taggctggag ctgcttc                                                     137


<210>  49
<211>  114
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence 2324230-2324131 of 
       the atoB gene (sequence 2324131 to 2325315) and a region 
       homologous to the RBS01*2 consensus sequence containing the PsiI 
       restriction site

<400>  49
ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag tgaaccgtta aaactaccga       60

tagcagtacg taccgcactg acgatgacac aatttttcat ttataacctc ctta            114


<210>  50
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli  sequence 2323667 to 2323685

<400>  50
gcgcggcagc acgcagtac                                                    19


<210>  51
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli  sequence 2324584 to 2324565

<400>  51
cgcacatcag gccatcgcgc                                                   20


<210>  52
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  primer udhA-NheIf

<400>  52
aattgctagc attatataca aggaggaaac agctatgcca cattcctacg attacgatg        59


<210>  53
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  primer udhA-xma1r

<400>  53
aattcccggg ataattttaa aacaggcggt ttaaaccgtt taac                        44


<210>  54
<211>  102
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (1486256-1486336) of 
       the aldA gene (sequence 1486256 to 1487695), and a region 
       (upper-case letters) for the amplification of the chloramphenicol
       resistance cassette of the pKD3 plasmid

<400>  54
atgtcagtac ccgttcaaca tcctatgtat atcgatggac agtttgttac ctggcgtgga       60

gacgcatgga ttgatgtggt agtgtaggct ggagctgctt cg                         102


<210>  55
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (1487615-1487695) 
       corresponding to the C terminal part encoding seq of the aldA 
       gene (1486256 to 1487695), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  55
ttaagactgt aaataaacca cctgggtctg cagatattca tgcaagccat gtttaccatc       60

tgcgccgcca ataccggatt tcatatgaat atcctcctta g                          101


<210>  56
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1485722 to 1485752

<400>  56
tgcagcggcg cacgatggcg acgttccgcc g                                      31


<210>  57
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1488195 to 1488225

<400>  57
cacgatgacg accattcatg cctatactgg c                                      31


<210>  58
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (3752996 to 3753075) 
       of the aldB gene (sequence 3752996  3754534), and a region for 
       the amplification of the chloramphenicol resistance cassette of 
       the pKD3 plasmid

<400>  58
tcagaacagc cccaacggtt tatccgagta gctcaccagc aggcacttgg tttgctggta       60

atgctccagc atcatcttgt gtgtaggctg gagctgcttc g                          101


<210>  59
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (3754455 to 3754534) 
       of the aldB gene (sequence 3752996  3754534), and a region 
       (upper-case letters) for the amplification of the chloramphenicol
       resistance cassette of the pKD3 plasmid

<400>  59
atgaccaata atcccccttc agcacagatt aagcccggcg agtatggttt ccccctcaag       60

ttaaaagccc gctatgacaa catatgaata tcctccttag                            100


<210>  60
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 3752449 to 3752488

<400>  60
catatttccc tcaaagaata taaaaaagaa caattaacgc                             40


<210>  61
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli  3755040 to 3755070

<400>  61
tatgttcatg cgatggcgca ccagctgggc g                                      31


<210>  62
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (3560036-3560115) of 
       the glpD gene (sequence 3560036 to 3561541), and a region 
       (upper-case letters) for the amplification of the chloramphenicol
       resistance cassette of the pKD3 plasmid

<400>  62
atggaaacca aagatctgat tgtgataggg ggcggcatca atggtgctgg tatcgcggca       60

gacgccgctg gacgcggttt catatgaata tcctccttag                            100


<210>  63
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer : a region homologous to the seq (3561462-3561541) 
       corresponding to the C terminal part encoding seq of the glpD 
       gene (3560036 to 3561541), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  63
ttacgacgcc agcgataacc tctgctgcgt atactccacc agccactgac tcacacgaga       60

ttgttgatcc gcatttagcc tgtaggctgg agctgcttcg                            100


<210>  64
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 3559822 to 3559844

<400>  64
cgttaataca ttcgaactga tcc                                               23


<210>  65
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 3561750 to 3561772

<400>  65
gcgtgggctt tgcggtaatt ccc                                               23


<210>  66
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer : a region homologous to the sequence (2350669-2350748) of
       the glpA gene (sequence 2350669 to 2352297), and a region for the
       amplification of the chloramphenicol resistance cassette of the 
       pKD3 plasmid

<400>  66
atgaaaactc gcgactcgca atcaagtgac gtgattatca ttggcggcgg cgcaacggga       60

gccgggattg cccgcgactg catatgaata tcctccttag                            100


<210>  67
<211>  98
<212>  DNA
<213>  artificial

<220>
<223>  primer : a region homologous to the seq (2352297-2352220) 
       corresponding to the C terminal part encoding seq of the glpA 
       gene (2350669 to 2352297), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  67
tcaaagcgca tctttctgct ccttctccag accacacaat ccctgataaa cccagcgggt       60

aaattcgctt tcgcgcagtg taggctggag ctgcttcg                               98


<210>  68
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 2349038 to 2349052

<400>  68
ttagcctccg ttgcgttctt gc                                                22


<210>  69
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 2352316 to 3352289

<400>  69
gccgcccata atgacagtat caaagcgc                                          28


<210>  70
<211>  61
<212>  DNA
<213>  artificial

<220>
<223>  primer pntAA-NheIf

<400>  70
aattgctagc attatataca aggaggaaac agctatgcga attggcatac caagagaacg       60

g                                                                       61


<210>  71
<211>  37
<212>  DNA
<213>  artificial

<220>
<223>  primer pntB-xma1r

<400>  71
aattcccggg ataattttac agagctttca ggattgc                                37


<210>  72
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  primer pdhaB1f: BamHI-RBS dhaB1

<400>  72
ggatccgtga ttggaggagt aaaaatgata agtaaagg                               38


<210>  73
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  primer pdhaB2r:XhoI-dhaB2

<400>  73
ctcgagttac tcagctccaa ttgtgcacgg tattcccat                              39


<210>  74
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  primer pyqhD1: XhoI RBS01 yqhD

<400>  74
ctcgagttat aacctcctta atgaacaact ttaatctgca caccccaacc cgcattct         58


<210>  75
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  primer pyqhD2 : SfoI yqhDrv

<400>  75
ggcgccttag cgggcggctt cgtatatacg gcggctgaca t                           41


<210>  76
<211>  102
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (4109530-4109449) of 
       the tpiA gene (sequence 4108763 to 4109530), and a region for the
       amplification of the chloramphenicol resistance cassette of the 
       pKD3 plasmid

<400>  76
atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac       60

gagctggttt ctaacctgcg tacatatgaa tatcctcctt ag                         102


<210>  77
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer:a region homologous to the seq (4108762-4108841) 
       corresponding to the C terminal part encoding seq of the tpiA 
       gene (4108763 to 4109530), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  77
cttaagcctg tttagccgct tctgcagctt taacgattac tgcgaaggcg tcagctttca       60

gagaagcacc accaaccagc tgtaggctgg agctgcttcg                            100


<210>  78
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence  4107979 to 4107998

<400>  78
ggtgatgata gttatcgccg                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 4110023 to 4110042

<400>  79
cgtgccatcg acagcagtcc                                                   20


<210>  80
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (1725861-1725940) of 
       the gloA gene (sequence 1725861 to 1726268), and a region for the
       amplification of the chloramphenicol resistance cassette of the 
       pKD3 plasmid

<400>  80
atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc aacgctccat cgatttttat       60

accaaagtgc tgggcatgaa gtgtaggctg gagctgcttc g                          101


<210>  81
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer:a region homologous to the seq (17261891726268) 
       corresponding to the C terminal part encoding seq of the gloA 
       gene (1725861 to 1726268), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  81
ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt aactcaattt tgtaaccgtc       60

cggatcttcc acaaacgcga catatgaata tcctccttag                            100


<210>  82
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli  sequence 1725331 to 1725361

<400>  82
gaagtggtcg atgccgggat tgaagaatgg g                                      31


<210>  83
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 1726765 to 1726795

<400>  83
gggttacgtt tcagtgaggc gcgttctgcg g                                      31


<210>  84
<211>  101
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (234703-234782) of 
       the gloB gene (sequence 234027 to 234782), and a region for the 
       amplification of the chloramphenicol resistance cassette of the 
       pKD3 plasmid

<400>  84
acaatcaggc agcgacctgc ttcatcattc aaaacccaga tgtaattgtc atcaaaggcg       60

ggaatactgt taagattcat gtgtaggctg gagctgcttc g                          101


<210>  85
<211>  102
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (234027-234108) 
       corresponding to the C terminal part encoding seq of the gloB 
       gene (234027 to 234782), and a region for the amplification of 
       the chloramphenicol resistance cassette carried by the pKD3 
       

<400>  85
taatgtaatt aatgaagaaa cattattgca acaacctgaa gagcgttttg catggttaag       60

gtcaaagaaa gataggttct gacatatgaa tatcctcctt ag                         102


<210>  86
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer : homologous to the E. coli sequence 233929 to 233950

<400>  86
ggcgagtaat atcgcttttg cc                                                22


<210>  87
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 234822 to 234845

<400>  87
gacagtttga gggactcttg ccgg                                              24


<210>  88
<211>  121
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the sequence (2033859-2033938) of 
       the hchA gene (sequence 2033859 to 2034710), and a region for the
       amplification of the chloramphenicol resistance cassette of the 
       pKD3 plasmid

<400>  88
atgactgttc aaacaagtaa aaatccgcag gtcgatattg ctgaagataa tgcattcttc       60

ccttcagaat attcgcttag ccttcagaat attcgcttag gtgtaggctg gagctgcttc      120

g                                                                      121


<210>  89
<211>  100
<212>  DNA
<213>  artificial

<220>
<223>  primer: a region homologous to the seq (2034631-2034710) 
       corresponding to the C terminal part encoding seq of the hchA 
       gene (2033859 to 2034710), and a region for the amplification of 
       the chloramphenicol resistance cassette of the pKD3 plasmid

<400>  89
ttaacccgcg taagctgcca gcatttcctg cgccgccagt ttacccaacg cattcgctgc       60

aaaaggactg tcgccggtga catatgaata tcctccttag                            100


<210>  90
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 2033243-2033264

<400>  90
tgtgggctta gtctaccagt tc                                                22


<210>  91
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer: homologous to the E. coli sequence 2034818 to 2034837

<400>  91
cgttaccgca aagaaattaa                                                   20


<210>  92
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  primer pgldA1f: BamHI-RBS gldA

<400>  92
ggatccttat aacctcctta atggaccgca ttattcaatc accggg                      46


<210>  93
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer pgldAr:XhoI-gldA

<400>  93
ctcgagttat tcccactctt gcaggaaacg c                                      31


<210>  94
<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  primer psadh1f : BamHI-RBSsadh

<400>  94
ggatcctatc tttaaggagg aacatatttt atgaaaggtt ttgcaatgc                   49


<210>  95
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  primer psadh2r:XhoI-sadh

<400>  95
ctcgagttat aatataacta ctgctttaat taagtctttt ggc                         43


