                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Pfizer Inc.
 
<120>  REVERSAL AGENTS FOR FXIa INHIBITORS

<130>  081906-1016798-222810PC

<150>  US 62/196,085
<151>  2015-07-23

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  607
<212>  PRT
<213>  Homo sapiens

<400>  1

Glu Cys Val Thr Gln Leu Leu Lys Asp Thr Cys Phe Glu Gly Gly Asp 
1               5                   10                  15      


Ile Thr Thr Val Phe Thr Pro Ser Ala Lys Tyr Cys Gln Val Val Cys 
            20                  25                  30          


Thr Tyr His Pro Arg Cys Leu Leu Phe Thr Phe Thr Ala Glu Ser Pro 
        35                  40                  45              


Ser Glu Asp Pro Thr Arg Trp Phe Thr Cys Val Leu Lys Asp Ser Val 
    50                  55                  60                  


Thr Glu Thr Leu Pro Arg Val Asn Arg Thr Ala Ala Ile Ser Gly Tyr 
65                  70                  75                  80  


Ser Phe Lys Gln Cys Ser His Gln Ile Ser Ala Cys Asn Lys Asp Ile 
                85                  90                  95      


Tyr Val Asp Leu Asp Met Lys Gly Ile Asn Tyr Asn Ser Ser Val Ala 
            100                 105                 110         


Lys Ser Ala Gln Glu Cys Gln Glu Arg Cys Thr Asp Asp Val His Cys 
        115                 120                 125             


His Phe Phe Thr Tyr Ala Thr Arg Gln Phe Pro Ser Leu Glu His Arg 
    130                 135                 140                 


Asn Ile Cys Leu Leu Lys His Thr Gln Thr Gly Thr Pro Thr Arg Ile 
145                 150                 155                 160 


Thr Lys Leu Asp Lys Val Val Ser Gly Phe Ser Leu Lys Ser Cys Ala 
                165                 170                 175     


Leu Ser Asn Leu Ala Cys Ile Arg Asp Ile Phe Pro Asn Thr Val Phe 
            180                 185                 190         


Ala Asp Ser Asn Ile Asp Ser Val Met Ala Pro Asp Ala Phe Val Cys 
        195                 200                 205             


Gly Arg Ile Cys Thr His His Pro Gly Cys Leu Phe Phe Thr Phe Phe 
    210                 215                 220                 


Ser Gln Glu Trp Pro Lys Glu Ser Gln Arg Asn Leu Cys Leu Leu Lys 
225                 230                 235                 240 


Thr Ser Glu Ser Gly Leu Pro Ser Thr Arg Ile Lys Lys Ser Lys Ala 
                245                 250                 255     


Leu Ser Gly Phe Ser Leu Gln Ser Cys Arg His Ser Ile Pro Val Phe 
            260                 265                 270         


Cys His Ser Ser Phe Tyr His Asp Thr Asp Phe Leu Gly Glu Glu Leu 
        275                 280                 285             


Asp Ile Val Ala Ala Lys Ser His Glu Ala Cys Gln Lys Leu Cys Thr 
    290                 295                 300                 


Asn Ala Val Arg Cys Gln Phe Phe Thr Tyr Thr Pro Ala Gln Ala Ser 
305                 310                 315                 320 


Cys Asn Glu Gly Lys Gly Lys Cys Tyr Leu Lys Leu Ser Ser Asn Gly 
                325                 330                 335     


Ser Pro Thr Lys Ile Leu His Gly Arg Gly Gly Ile Ser Gly Tyr Thr 
            340                 345                 350         


Leu Arg Leu Cys Lys Met Asp Asn Glu Cys Thr Thr Lys Ile Lys Pro 
        355                 360                 365             


Arg Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro Trp Gln 
    370                 375                 380                 


Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys Gly Gly 
385                 390                 395                 400 


Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys Phe Tyr 
                405                 410                 415     


Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile Leu Asn 
            420                 425                 430         


Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln Glu Ile 
        435                 440                 445             


Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp Ile Ala 
    450                 455                 460                 


Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln Arg Pro 
465                 470                 475                 480 


Ile Cys Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr Asp Cys 
                485                 490                 495     


Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile Gln Asn 
            500                 505                 510         


Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu Cys Gln 
        515                 520                 525             


Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys Ala Gly 
    530                 535                 540                 


Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ser Gly Gly Pro 
545                 550                 555                 560 


Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile Thr Ser 
                565                 570                 575     


Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr Thr Asn 
            580                 585                 590         


Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala Val 
        595                 600                 605         


<210>  2
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic FXIS557A catalytic domain mature protein sequence

<400>  2

Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro Trp Gln Val 
1               5                   10                  15      


Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys Gly Gly Ser 
            20                  25                  30          


Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys Phe Tyr Gly 
        35                  40                  45              


Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile Leu Asn Gln 
    50                  55                  60                  


Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln Glu Ile Ile 
65                  70                  75                  80  


Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp Ile Ala Leu 
                85                  90                  95      


Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln Arg Pro Ile 
            100                 105                 110         


Ser Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr Asp Cys Trp 
        115                 120                 125             


Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile Gln Asn Thr 
    130                 135                 140                 


Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu Cys Gln Lys 
145                 150                 155                 160 


Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys Ala Gly Tyr 
                165                 170                 175     


Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ala Gly Gly Pro Leu 
            180                 185                 190         


Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile Thr Ser Trp 
        195                 200                 205             


Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr Thr Asn Val 
    210                 215                 220                 


Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala 
225                 230                 235         


<210>  3
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence encoding FXIS557A catalytic domain 
       mature protein sequence

<400>  3
atcgttggag gaactgcgtc tgttcgtggt gagtggccgt ggcaggtgac cctgcacaca       60

acctcaccca ctcagagaca cctgtgtgga ggctccatca ttggaaacca gtggatatta      120

acagccgctc actgtttcta tggggtagag tcacctaaga ttttgcgtgt ctacagtggc      180

attttaaatc aatctgaaat aaaagaggac acatctttct ttggggttca agaaataata      240

atccatgatc agtataaaat ggcagaaagc gggtatgata ttgccttgtt gaaactggaa      300

accacagtga attacacaga ttctcaacga cccataagcc tgccttccaa aggagataga      360

aatgtaatat acactgattg ctgggtgact ggatgggggt acagaaaact aagagacaaa      420

atacaaaata ctctccagaa agccaagata cccttagtga ccaacgaaga gtgccagaag      480

agatacagag gacataaaat aacccataag atgatctgtg ccggctacag ggaaggaggg      540

aaggacgctt gcaagggaga tgcgggaggc cctctgtcct gcaaacacaa tgaggtctgg      600

catctggtag gcatcacgag ctggggcgaa ggctgtgctc aaagggagcg gccaggtgtt      660

tacaccaacg tggtcgagta cgtggactgg attctggaga aaactcaagc a               711


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Signal peptide amino acid sequence

<400>  4

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  5
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Signal peptide nucleotide sequence

<400>  5
atgggctggt cctgtatcat cctctttctg gtggccacag ctaccggagt gcatagc          57


<210>  6
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FXIS557A catalytic domain protein sequence with signal peptide 
       and His tag

<400>  6

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro 
            20                  25                  30          


Trp Gln Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys 
        35                  40                  45              


Gly Gly Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys 
    50                  55                  60                  


Phe Tyr Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile 
65                  70                  75                  80  


Leu Asn Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln 
                85                  90                  95      


Glu Ile Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp 
            100                 105                 110         


Ile Ala Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln 
        115                 120                 125             


Arg Pro Ile Ser Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr 
    130                 135                 140                 


Asp Cys Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile 
145                 150                 155                 160 


Gln Asn Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu 
                165                 170                 175     


Cys Gln Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys 
            180                 185                 190         


Ala Gly Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ala Gly 
        195                 200                 205             


Gly Pro Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile 
    210                 215                 220                 


Thr Ser Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr 
225                 230                 235                 240 


Thr Asn Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala 
                245                 250                 255     


His His His His His His His His 
            260                 


<210>  7
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic FXIS557A catalytic domain protein sequence with signal 
       peptide

<400>  7

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro 
            20                  25                  30          


Trp Gln Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys 
        35                  40                  45              


Gly Gly Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys 
    50                  55                  60                  


Phe Tyr Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile 
65                  70                  75                  80  


Leu Asn Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln 
                85                  90                  95      


Glu Ile Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp 
            100                 105                 110         


Ile Ala Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln 
        115                 120                 125             


Arg Pro Ile Ser Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr 
    130                 135                 140                 


Asp Cys Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile 
145                 150                 155                 160 


Gln Asn Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu 
                165                 170                 175     


Cys Gln Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys 
            180                 185                 190         


Ala Gly Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ala Gly 
        195                 200                 205             


Gly Pro Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile 
    210                 215                 220                 


Thr Ser Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr 
225                 230                 235                 240 


Thr Asn Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala 
                245                 250                 255     


<210>  8
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence encoding  FXIS557A catalytic domain
       mature protein sequence and an N-terminal signal peptide of SEQ 
       ID NO:4.

<400>  8
atgggctggt cctgtatcat cctctttctg gtggccacag ctaccggagt gcatagcatc       60

gttggaggaa ctgcgtctgt tcgtggtgag tggccgtggc aggtgaccct gcacacaacc      120

tcacccactc agagacacct gtgtggaggc tccatcattg gaaaccagtg gatattaaca      180

gccgctcact gtttctatgg ggtagagtca cctaagattt tgcgtgtcta cagtggcatt      240

ttaaatcaat ctgaaataaa agaggacaca tctttctttg gggttcaaga aataataatc      300

catgatcagt ataaaatggc agaaagcggg tatgatattg ccttgttgaa actggaaacc      360

acagtgaatt acacagattc tcaacgaccc ataagcctgc cttccaaagg agatagaaat      420

gtaatataca ctgattgctg ggtgactgga tgggggtaca gaaaactaag agacaaaata      480

caaaatactc tccagaaagc caagataccc ttagtgacca acgaagagtg ccagaagaga      540

tacagaggac ataaaataac ccataagatg atctgtgccg gctacaggga aggagggaag      600

gacgcttgca agggagatgc gggaggccct ctgtcctgca aacacaatga ggtctggcat      660

ctggtaggca tcacgagctg gggcgaaggc tgtgctcaaa gggagcggcc aggtgtttac      720

accaacgtgg tcgagtacgt ggactggatt ctggagaaaa ctcaagca                   768


<210>  9
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic DEF antibody VH sequence

<400>  9

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asp Pro Asp Glu Gly Asp Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Leu Ala Ser Gly Phe Arg Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  10
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic DEF antibody VL sequence

<400>  10

Asp Ile Val Met Thr Lys Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 
            20                  25                  30          


Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln His Asp Ile Tyr Ala Ser 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg 
            100                 105             


<210>  11
<211>  607
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Full length FXIS557A protein with mutated residue at 
       position 557

<400>  11

Glu Cys Val Thr Gln Leu Leu Lys Asp Thr Cys Phe Glu Gly Gly Asp 
1               5                   10                  15      


Ile Thr Thr Val Phe Thr Pro Ser Ala Lys Tyr Cys Gln Val Val Cys 
            20                  25                  30          


Thr Tyr His Pro Arg Cys Leu Leu Phe Thr Phe Thr Ala Glu Ser Pro 
        35                  40                  45              


Ser Glu Asp Pro Thr Arg Trp Phe Thr Cys Val Leu Lys Asp Ser Val 
    50                  55                  60                  


Thr Glu Thr Leu Pro Arg Val Asn Arg Thr Ala Ala Ile Ser Gly Tyr 
65                  70                  75                  80  


Ser Phe Lys Gln Cys Ser His Gln Ile Ser Ala Cys Asn Lys Asp Ile 
                85                  90                  95      


Tyr Val Asp Leu Asp Met Lys Gly Ile Asn Tyr Asn Ser Ser Val Ala 
            100                 105                 110         


Lys Ser Ala Gln Glu Cys Gln Glu Arg Cys Thr Asp Asp Val His Cys 
        115                 120                 125             


His Phe Phe Thr Tyr Ala Thr Arg Gln Phe Pro Ser Leu Glu His Arg 
    130                 135                 140                 


Asn Ile Cys Leu Leu Lys His Thr Gln Thr Gly Thr Pro Thr Arg Ile 
145                 150                 155                 160 


Thr Lys Leu Asp Lys Val Val Ser Gly Phe Ser Leu Lys Ser Cys Ala 
                165                 170                 175     


Leu Ser Asn Leu Ala Cys Ile Arg Asp Ile Phe Pro Asn Thr Val Phe 
            180                 185                 190         


Ala Asp Ser Asn Ile Asp Ser Val Met Ala Pro Asp Ala Phe Val Cys 
        195                 200                 205             


Gly Arg Ile Cys Thr His His Pro Gly Cys Leu Phe Phe Thr Phe Phe 
    210                 215                 220                 


Ser Gln Glu Trp Pro Lys Glu Ser Gln Arg Asn Leu Cys Leu Leu Lys 
225                 230                 235                 240 


Thr Ser Glu Ser Gly Leu Pro Ser Thr Arg Ile Lys Lys Ser Lys Ala 
                245                 250                 255     


Leu Ser Gly Phe Ser Leu Gln Ser Cys Arg His Ser Ile Pro Val Phe 
            260                 265                 270         


Cys His Ser Ser Phe Tyr His Asp Thr Asp Phe Leu Gly Glu Glu Leu 
        275                 280                 285             


Asp Ile Val Ala Ala Lys Ser His Glu Ala Cys Gln Lys Leu Cys Thr 
    290                 295                 300                 


Asn Ala Val Arg Cys Gln Phe Phe Thr Tyr Thr Pro Ala Gln Ala Ser 
305                 310                 315                 320 


Cys Asn Glu Gly Lys Gly Lys Cys Tyr Leu Lys Leu Ser Ser Asn Gly 
                325                 330                 335     


Ser Pro Thr Lys Ile Leu His Gly Arg Gly Gly Ile Ser Gly Tyr Thr 
            340                 345                 350         


Leu Arg Leu Cys Lys Met Asp Asn Glu Cys Thr Thr Lys Ile Lys Pro 
        355                 360                 365             


Arg Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro Trp Gln 
    370                 375                 380                 


Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys Gly Gly 
385                 390                 395                 400 


Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys Phe Tyr 
                405                 410                 415     


Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile Leu Asn 
            420                 425                 430         


Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln Glu Ile 
        435                 440                 445             


Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp Ile Ala 
    450                 455                 460                 


Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln Arg Pro 
465                 470                 475                 480 


Ile Cys Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr Asp Cys 
                485                 490                 495     


Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile Gln Asn 
            500                 505                 510         


Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu Cys Gln 
        515                 520                 525             


Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys Ala Gly 
    530                 535                 540                 


Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ala Gly Gly Pro 
545                 550                 555                 560 


Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile Thr Ser 
                565                 570                 575     


Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr Thr Asn 
            580                 585                 590         


Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala Val 
        595                 600                 605         


<210>  12
<211>  607
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Full length FXIS557A protein sequence with mutated 
       residue at position 482

<400>  12

Glu Cys Val Thr Gln Leu Leu Lys Asp Thr Cys Phe Glu Gly Gly Asp 
1               5                   10                  15      


Ile Thr Thr Val Phe Thr Pro Ser Ala Lys Tyr Cys Gln Val Val Cys 
            20                  25                  30          


Thr Tyr His Pro Arg Cys Leu Leu Phe Thr Phe Thr Ala Glu Ser Pro 
        35                  40                  45              


Ser Glu Asp Pro Thr Arg Trp Phe Thr Cys Val Leu Lys Asp Ser Val 
    50                  55                  60                  


Thr Glu Thr Leu Pro Arg Val Asn Arg Thr Ala Ala Ile Ser Gly Tyr 
65                  70                  75                  80  


Ser Phe Lys Gln Cys Ser His Gln Ile Ser Ala Cys Asn Lys Asp Ile 
                85                  90                  95      


Tyr Val Asp Leu Asp Met Lys Gly Ile Asn Tyr Asn Ser Ser Val Ala 
            100                 105                 110         


Lys Ser Ala Gln Glu Cys Gln Glu Arg Cys Thr Asp Asp Val His Cys 
        115                 120                 125             


His Phe Phe Thr Tyr Ala Thr Arg Gln Phe Pro Ser Leu Glu His Arg 
    130                 135                 140                 


Asn Ile Cys Leu Leu Lys His Thr Gln Thr Gly Thr Pro Thr Arg Ile 
145                 150                 155                 160 


Thr Lys Leu Asp Lys Val Val Ser Gly Phe Ser Leu Lys Ser Cys Ala 
                165                 170                 175     


Leu Ser Asn Leu Ala Cys Ile Arg Asp Ile Phe Pro Asn Thr Val Phe 
            180                 185                 190         


Ala Asp Ser Asn Ile Asp Ser Val Met Ala Pro Asp Ala Phe Val Cys 
        195                 200                 205             


Gly Arg Ile Cys Thr His His Pro Gly Cys Leu Phe Phe Thr Phe Phe 
    210                 215                 220                 


Ser Gln Glu Trp Pro Lys Glu Ser Gln Arg Asn Leu Cys Leu Leu Lys 
225                 230                 235                 240 


Thr Ser Glu Ser Gly Leu Pro Ser Thr Arg Ile Lys Lys Ser Lys Ala 
                245                 250                 255     


Leu Ser Gly Phe Ser Leu Gln Ser Cys Arg His Ser Ile Pro Val Phe 
            260                 265                 270         


Cys His Ser Ser Phe Tyr His Asp Thr Asp Phe Leu Gly Glu Glu Leu 
        275                 280                 285             


Asp Ile Val Ala Ala Lys Ser His Glu Ala Cys Gln Lys Leu Cys Thr 
    290                 295                 300                 


Asn Ala Val Arg Cys Gln Phe Phe Thr Tyr Thr Pro Ala Gln Ala Ser 
305                 310                 315                 320 


Cys Asn Glu Gly Lys Gly Lys Cys Tyr Leu Lys Leu Ser Ser Asn Gly 
                325                 330                 335     


Ser Pro Thr Lys Ile Leu His Gly Arg Gly Gly Ile Ser Gly Tyr Thr 
            340                 345                 350         


Leu Arg Leu Cys Lys Met Asp Asn Glu Cys Thr Thr Lys Ile Lys Pro 
        355                 360                 365             


Arg Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro Trp Gln 
    370                 375                 380                 


Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys Gly Gly 
385                 390                 395                 400 


Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys Phe Tyr 
                405                 410                 415     


Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile Leu Asn 
            420                 425                 430         


Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln Glu Ile 
        435                 440                 445             


Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp Ile Ala 
    450                 455                 460                 


Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln Arg Pro 
465                 470                 475                 480 


Ile Ser Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr Asp Cys 
                485                 490                 495     


Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile Gln Asn 
            500                 505                 510         


Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu Cys Gln 
        515                 520                 525             


Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys Ala Gly 
    530                 535                 540                 


Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ser Gly Gly Pro 
545                 550                 555                 560 


Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile Thr Ser 
                565                 570                 575     


Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr Thr Asn 
            580                 585                 590         


Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala Val 
        595                 600                 605         


<210>  13
<211>  607
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Full length FXIS557A protein sequence with mutated 
       residues at positions 557 and 482

<400>  13

Glu Cys Val Thr Gln Leu Leu Lys Asp Thr Cys Phe Glu Gly Gly Asp 
1               5                   10                  15      


Ile Thr Thr Val Phe Thr Pro Ser Ala Lys Tyr Cys Gln Val Val Cys 
            20                  25                  30          


Thr Tyr His Pro Arg Cys Leu Leu Phe Thr Phe Thr Ala Glu Ser Pro 
        35                  40                  45              


Ser Glu Asp Pro Thr Arg Trp Phe Thr Cys Val Leu Lys Asp Ser Val 
    50                  55                  60                  


Thr Glu Thr Leu Pro Arg Val Asn Arg Thr Ala Ala Ile Ser Gly Tyr 
65                  70                  75                  80  


Ser Phe Lys Gln Cys Ser His Gln Ile Ser Ala Cys Asn Lys Asp Ile 
                85                  90                  95      


Tyr Val Asp Leu Asp Met Lys Gly Ile Asn Tyr Asn Ser Ser Val Ala 
            100                 105                 110         


Lys Ser Ala Gln Glu Cys Gln Glu Arg Cys Thr Asp Asp Val His Cys 
        115                 120                 125             


His Phe Phe Thr Tyr Ala Thr Arg Gln Phe Pro Ser Leu Glu His Arg 
    130                 135                 140                 


Asn Ile Cys Leu Leu Lys His Thr Gln Thr Gly Thr Pro Thr Arg Ile 
145                 150                 155                 160 


Thr Lys Leu Asp Lys Val Val Ser Gly Phe Ser Leu Lys Ser Cys Ala 
                165                 170                 175     


Leu Ser Asn Leu Ala Cys Ile Arg Asp Ile Phe Pro Asn Thr Val Phe 
            180                 185                 190         


Ala Asp Ser Asn Ile Asp Ser Val Met Ala Pro Asp Ala Phe Val Cys 
        195                 200                 205             


Gly Arg Ile Cys Thr His His Pro Gly Cys Leu Phe Phe Thr Phe Phe 
    210                 215                 220                 


Ser Gln Glu Trp Pro Lys Glu Ser Gln Arg Asn Leu Cys Leu Leu Lys 
225                 230                 235                 240 


Thr Ser Glu Ser Gly Leu Pro Ser Thr Arg Ile Lys Lys Ser Lys Ala 
                245                 250                 255     


Leu Ser Gly Phe Ser Leu Gln Ser Cys Arg His Ser Ile Pro Val Phe 
            260                 265                 270         


Cys His Ser Ser Phe Tyr His Asp Thr Asp Phe Leu Gly Glu Glu Leu 
        275                 280                 285             


Asp Ile Val Ala Ala Lys Ser His Glu Ala Cys Gln Lys Leu Cys Thr 
    290                 295                 300                 


Asn Ala Val Arg Cys Gln Phe Phe Thr Tyr Thr Pro Ala Gln Ala Ser 
305                 310                 315                 320 


Cys Asn Glu Gly Lys Gly Lys Cys Tyr Leu Lys Leu Ser Ser Asn Gly 
                325                 330                 335     


Ser Pro Thr Lys Ile Leu His Gly Arg Gly Gly Ile Ser Gly Tyr Thr 
            340                 345                 350         


Leu Arg Leu Cys Lys Met Asp Asn Glu Cys Thr Thr Lys Ile Lys Pro 
        355                 360                 365             


Arg Ile Val Gly Gly Thr Ala Ser Val Arg Gly Glu Trp Pro Trp Gln 
    370                 375                 380                 


Val Thr Leu His Thr Thr Ser Pro Thr Gln Arg His Leu Cys Gly Gly 
385                 390                 395                 400 


Ser Ile Ile Gly Asn Gln Trp Ile Leu Thr Ala Ala His Cys Phe Tyr 
                405                 410                 415     


Gly Val Glu Ser Pro Lys Ile Leu Arg Val Tyr Ser Gly Ile Leu Asn 
            420                 425                 430         


Gln Ser Glu Ile Lys Glu Asp Thr Ser Phe Phe Gly Val Gln Glu Ile 
        435                 440                 445             


Ile Ile His Asp Gln Tyr Lys Met Ala Glu Ser Gly Tyr Asp Ile Ala 
    450                 455                 460                 


Leu Leu Lys Leu Glu Thr Thr Val Asn Tyr Thr Asp Ser Gln Arg Pro 
465                 470                 475                 480 


Ile Ser Leu Pro Ser Lys Gly Asp Arg Asn Val Ile Tyr Thr Asp Cys 
                485                 490                 495     


Trp Val Thr Gly Trp Gly Tyr Arg Lys Leu Arg Asp Lys Ile Gln Asn 
            500                 505                 510         


Thr Leu Gln Lys Ala Lys Ile Pro Leu Val Thr Asn Glu Glu Cys Gln 
        515                 520                 525             


Lys Arg Tyr Arg Gly His Lys Ile Thr His Lys Met Ile Cys Ala Gly 
    530                 535                 540                 


Tyr Arg Glu Gly Gly Lys Asp Ala Cys Lys Gly Asp Ala Gly Gly Pro 
545                 550                 555                 560 


Leu Ser Cys Lys His Asn Glu Val Trp His Leu Val Gly Ile Thr Ser 
                565                 570                 575     


Trp Gly Glu Gly Cys Ala Gln Arg Glu Arg Pro Gly Val Tyr Thr Asn 
            580                 585                 590         


Val Val Glu Tyr Val Asp Trp Ile Leu Glu Lys Thr Gln Ala Val 
        595                 600                 605         


