                         SEQUENCE LISTING

<110>  Novozymes A/S
       
 
<120>  POLYPEPTIDES HAVING PULLULANASE ACTIVITY SUITABLE FOR USE IN 
       LIQUEFACTION

<130>  13195-WO-PCT

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  829
<212>  PRT
<213>  Bacillus sp-17840

<400>  1

Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp Ser 
1               5                   10                  15      


Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val Asn 
            20                  25                  30          


Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly Val 
        35                  40                  45              


Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu Ile 
    50                  55                  60                  


Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu His 
65                  70                  75                  80  


Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp Pro 
                85                  90                  95      


Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro Lys 
            100                 105                 110         


Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys Leu 
        115                 120                 125             


Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val Thr 
    130                 135                 140                 


Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn Ala 
145                 150                 155                 160 


Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr Leu 
                165                 170                 175     


Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala Gly 
            180                 185                 190         


Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro Arg 
        195                 200                 205             


Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys Ala 
    210                 215                 220                 


Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu Leu 
225                 230                 235                 240 


Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met Gln 
                245                 250                 255     


Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu Lys 
            260                 265                 270         


Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln Thr 
        275                 280                 285             


Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg Gly 
    290                 295                 300                 


Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu Asp 
305                 310                 315                 320 


His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu Val 
                325                 330                 335     


His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn Lys 
            340                 345                 350         


Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp Asn 
        355                 360                 365             


Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala Val 
    370                 375                 380                 


Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln Pro 
385                 390                 395                 400 


Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro Glu 
                405                 410                 415     


Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln Leu 
            420                 425                 430         


Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn Met 
        435                 440                 445             


Asp Val Val Tyr Asn His Thr Phe Asn Val Gly Val Ser Asp Phe Asp 
    450                 455                 460                 


Lys Ile Val Pro Gln Tyr Tyr Tyr Arg Thr Asp Ser Ala Gly Asn Tyr 
465                 470                 475                 480 


Thr Asn Gly Ser Gly Val Gly Asn Glu Ile Ala Thr Glu Arg Pro Met 
                485                 490                 495     


Val Gln Lys Phe Val Leu Asp Ser Val Lys Tyr Trp Val Lys Glu Tyr 
            500                 505                 510         


His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys Asp 
        515                 520                 525             


Thr Met Ala Lys Ile Ser Lys Glu Leu His Ala Ile Asn Pro Gly Ile 
    530                 535                 540                 


Val Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Gly Leu Ser Ser 
545                 550                 555                 560 


Asp Gln Leu Val Thr Lys Gly Gln Gln Lys Gly Leu Gly Ile Gly Val 
                565                 570                 575     


Phe Asn Asp Asn Ile Arg Asn Gly Leu Asp Gly Asn Val Phe Asp Lys 
            580                 585                 590         


Ser Ala Gln Gly Phe Ala Thr Gly Asp Pro Asn Gln Val Asn Val Ile 
        595                 600                 605             


Lys Asn Gly Val Met Gly Ser Ile Ser Asp Phe Thr Ser Ala Pro Ser 
    610                 615                 620                 


Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Met Thr Leu Trp Asp 
625                 630                 635                 640 


Lys Ile Ser Ala Ser Asn Pro Asn Asp Thr Gln Ala Asp Arg Ile Lys 
                645                 650                 655     


Met Asp Glu Leu Ala Gln Ala Val Val Phe Thr Ser Gln Gly Val Pro 
            660                 665                 670         


Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn Asp 
        675                 680                 685             


Asn Ser Tyr Asn Ala Gly Asp Ser Val Asn Gln Phe Asp Trp Ser Arg 
    690                 695                 700                 


Lys Ala Gln Phe Glu Asn Val Phe Asp Tyr Tyr Ser Trp Leu Ile His 
705                 710                 715                 720 


Leu Arg Asp Asn His Pro Ala Phe Arg Met Thr Thr Ala Asp Gln Ile 
                725                 730                 735     


Lys Gln Asn Leu Thr Phe Leu Asp Ser Pro Thr Asn Thr Val Ala Phe 
            740                 745                 750         


Glu Leu Lys Asn His Ala Asn His Asp Lys Trp Lys Asn Ile Ile Val 
        755                 760                 765             


Met Tyr Asn Pro Asn Lys Thr Ala Gln Thr Leu Thr Leu Pro Ser Gly 
    770                 775                 780                 


Asn Trp Thr Ile Val Gly Leu Gly Asn Gln Val Gly Glu Lys Ser Leu 
785                 790                 795                 800 


Gly His Val Asn Gly Thr Val Glu Val Pro Ala Leu Ser Thr Ile Ile 
                805                 810                 815     


Leu His Gln Gly Thr Ser Glu Asp Val Ile Asp Gln Asn 
            820                 825                 


<210>  2
<211>  928
<212>  PRT
<213>  Bacillus deramificans

<400>  2

Asp Gly Asn Thr Thr Thr Ile Ile Val His Tyr Phe Arg Pro Ala Gly 
1               5                   10                  15      


Asp Tyr Gln Pro Trp Ser Leu Trp Met Trp Pro Glu Gly Gly Ser Gly 
            20                  25                  30          


Ala Glu Tyr Asp Phe Asn Gly Thr Asp Ser Tyr Gly Glu Val Ala Asn 
        35                  40                  45              


Val Ser Ile Pro Gly Asn Pro Ser Gln Val Gly Ile Ile Val Arg Thr 
    50                  55                  60                  


Gln Asp Trp Thr Lys Asp Val Ser Ala Asp Arg Tyr Ile Asp Leu Ser 
65                  70                  75                  80  


Lys Gly His Glu Val Trp Leu Val Gln Gly Asn Ser Gln Ile Phe Tyr 
                85                  90                  95      


Asn Glu Lys Asp Ala Glu Asp Ala Ala Lys Pro Ala Val Ser Asn Ala 
            100                 105                 110         


Tyr Leu Asp Ala Ser Asn Gln Val Leu Val Lys Leu Ser Gln Pro Phe 
        115                 120                 125             


Thr Leu Gly Glu Gly Ala Ser Gly Phe Thr Val His Asp Asp Thr Val 
    130                 135                 140                 


Asn Lys Asp Ile Pro Val Thr Ser Val Thr Asp Ala Ser Leu Gly Gln 
145                 150                 155                 160 


Asn Val Thr Ala Val Leu Ala Gly Thr Phe Gln His Ile Phe Gly Gly 
                165                 170                 175     


Ser Asp Trp Ala Pro Asp Asn His Ser Thr Leu Leu Lys Lys Val Asn 
            180                 185                 190         


Asn Asn Leu Tyr Gln Phe Ser Gly Asp Leu Pro Glu Gly Asn Tyr Gln 
        195                 200                 205             


Tyr Lys Val Ala Leu Asn Asp Ser Trp Asn Asn Pro Ser Tyr Pro Ser 
    210                 215                 220                 


Asn Asn Ile Asp Leu Thr Val Pro Thr Gly Gly Ala His Val Thr Phe 
225                 230                 235                 240 


Ser Tyr Val Pro Ser Thr His Ala Val Tyr Asp Ser Ile Asn Asn Pro 
                245                 250                 255     


Gly Ala Asp Leu Pro Val Asn Gly Ser Gly Val Lys Thr Asp Leu Val 
            260                 265                 270         


Thr Val Thr Leu Gly Glu Asp Pro Asp Val Ser His Thr Leu Ser Ile 
        275                 280                 285             


Gln Thr Asp Gly Tyr Gln Ala Lys Gln Val Ile Ser Arg Asn Val Leu 
    290                 295                 300                 


Asp Ser Ser Gln Tyr Tyr Tyr Ser Gly Asp Asp Leu Gly Asn Thr Tyr 
305                 310                 315                 320 


Thr His Lys Ala Thr Thr Phe Lys Val Trp Ala Pro Thr Ser Thr Gln 
                325                 330                 335     


Val Asn Val Leu Leu Tyr Asn Ser Ala Thr Gly Ser Val Thr Lys Thr 
            340                 345                 350         


Val Pro Met Thr Ala Ser Gly His Gly Val Trp Glu Ala Thr Val Asn 
        355                 360                 365             


Gln Asn Leu Glu Asn Trp Tyr Tyr Met Tyr Glu Val Thr Gly Gln Gly 
    370                 375                 380                 


Ser Thr Arg Thr Ala Val Asp Pro Tyr Ala Thr Ala Ile Ala Pro Asn 
385                 390                 395                 400 


Gly Thr Arg Gly Met Ile Val Asp Leu Ala Lys Thr Asp Pro Ala Gly 
                405                 410                 415     


Trp Asn Ser Asp Lys His Ile Thr Pro Lys Asn Ile Glu Asp Glu Val 
            420                 425                 430         


Ile Tyr Glu Met Asp Val Arg Asp Phe Ser Ile Asp Pro Asn Ser Gly 
        435                 440                 445             


Met Lys Asn Lys Gly Lys Tyr Leu Ala Leu Thr Glu Lys Gly Thr Lys 
    450                 455                 460                 


Gly Pro Asp Asn Val Lys Thr Gly Ile Asp Ser Leu Lys Gln Leu Gly 
465                 470                 475                 480 


Ile Thr His Val Gln Leu Met Pro Val Phe Ala Phe Asn Ser Val Asp 
                485                 490                 495     


Glu Thr Asp Pro Thr Gln Asp Asn Trp Gly Tyr Asp Pro Arg Asn Tyr 
            500                 505                 510         


Asp Val Pro Glu Gly Gln Tyr Ala Thr Asn Ala Asn Gly Thr Ala Arg 
        515                 520                 525             


Ile Lys Glu Phe Lys Glu Met Val Leu Ser Leu His Arg Glu His Ile 
    530                 535                 540                 


Gly Val Asn Met Asp Val Val Tyr Asn His Thr Phe Ala Thr Gln Ile 
545                 550                 555                 560 


Ser Asp Phe Asp Lys Ile Val Pro Glu Tyr Tyr Tyr Arg Thr Asp Asp 
                565                 570                 575     


Ala Gly Asn Tyr Thr Asn Gly Ser Gly Thr Gly Asn Glu Ile Ala Ala 
            580                 585                 590         


Glu Arg Pro Met Val Gln Lys Phe Ile Ile Asp Ser Leu Lys Tyr Trp 
        595                 600                 605             


Val Asn Glu Tyr His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu 
    610                 615                 620                 


Leu Gly Lys Asp Thr Met Ser Lys Ala Ala Ser Glu Leu His Ala Ile 
625                 630                 635                 640 


Asn Pro Gly Ile Ala Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser 
                645                 650                 655     


Ala Leu Pro Glu Asp Gln Leu Leu Thr Lys Gly Ala Gln Lys Gly Met 
            660                 665                 670         


Gly Val Ala Val Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn 
        675                 680                 685             


Val Phe Asp Ser Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu 
    690                 695                 700                 


Thr Asp Ala Ile Lys Asn Gly Val Glu Gly Ser Ile Asn Asp Phe Thr 
705                 710                 715                 720 


Ser Ser Pro Gly Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Tyr 
                725                 730                 735     


Thr Leu Trp Asp Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala 
            740                 745                 750         


Asp Arg Ile Lys Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser 
        755                 760                 765             


Gln Gly Val Pro Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys 
    770                 775                 780                 


Gly Gly Asn Asp Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe 
785                 790                 795                 800 


Asp Trp Ser Arg Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser 
                805                 810                 815     


Gly Leu Ile His Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr 
            820                 825                 830         


Ala Asn Glu Ile Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn 
        835                 840                 845             


Thr Val Ala Tyr Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly 
    850                 855                 860                 


Asn Ile Ile Val Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn 
865                 870                 875                 880 


Leu Pro Ser Gly Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly 
                885                 890                 895     


Glu Ser Thr Leu Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile 
            900                 905                 910         


Ser Met Met Ile Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
        915                 920                 925             


<210>  3
<211>  828
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid pullulanase

<400>  3

Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp Ser 
1               5                   10                  15      


Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val Asn 
            20                  25                  30          


Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly Val 
        35                  40                  45              


Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu Ile 
    50                  55                  60                  


Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu His 
65                  70                  75                  80  


Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp Pro 
                85                  90                  95      


Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro Lys 
            100                 105                 110         


Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys Leu 
        115                 120                 125             


Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val Thr 
    130                 135                 140                 


Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn Ala 
145                 150                 155                 160 


Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr Leu 
                165                 170                 175     


Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala Gly 
            180                 185                 190         


Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro Arg 
        195                 200                 205             


Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys Ala 
    210                 215                 220                 


Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu Leu 
225                 230                 235                 240 


Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met Gln 
                245                 250                 255     


Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu Lys 
            260                 265                 270         


Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln Thr 
        275                 280                 285             


Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg Gly 
    290                 295                 300                 


Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu Asp 
305                 310                 315                 320 


His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu Val 
                325                 330                 335     


His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn Lys 
            340                 345                 350         


Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp Asn 
        355                 360                 365             


Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala Val 
    370                 375                 380                 


Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln Pro 
385                 390                 395                 400 


Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro Glu 
                405                 410                 415     


Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln Leu 
            420                 425                 430         


Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn Met 
        435                 440                 445             


Asp Val Val Tyr Asn His Thr Phe Ala Thr Gln Ile Ser Asp Phe Asp 
    450                 455                 460                 


Lys Ile Val Pro Glu Tyr Tyr Tyr Arg Thr Asp Asp Ala Gly Asn Tyr 
465                 470                 475                 480 


Thr Asn Gly Ser Gly Thr Gly Asn Glu Ile Ala Ala Glu Arg Pro Met 
                485                 490                 495     


Val Gln Lys Phe Ile Ile Asp Ser Leu Lys Tyr Trp Val Asn Glu Tyr 
            500                 505                 510         


His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys Asp 
        515                 520                 525             


Thr Met Ser Lys Ala Ala Ser Glu Leu His Ala Ile Asn Pro Gly Ile 
    530                 535                 540                 


Ala Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Ala Leu Pro Glu 
545                 550                 555                 560 


Asp Gln Leu Leu Thr Lys Gly Ala Gln Lys Gly Met Gly Val Ala Val 
                565                 570                 575     


Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn Val Phe Asp Ser 
            580                 585                 590         


Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala Ile 
        595                 600                 605             


Lys Asn Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro Gly 
    610                 615                 620                 


Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Tyr Thr Leu Trp Asp 
625                 630                 635                 640 


Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile Lys 
                645                 650                 655     


Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val Pro 
            660                 665                 670         


Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn Asp 
        675                 680                 685             


Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe Asp Trp Ser Arg 
    690                 695                 700                 


Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile His 
705                 710                 715                 720 


Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu Ile 
                725                 730                 735     


Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala Tyr 
            740                 745                 750         


Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile Val 
        755                 760                 765             


Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser Gly 
    770                 775                 780                 


Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Ser Thr Leu 
785                 790                 795                 800 


Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met Ile 
                805                 810                 815     


Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
            820                 825             


<210>  4
<211>  2586
<212>  DNA
<213>  Artificial

<220>
<223>  Hybrid pullulanase CDS

<400>  4
atgtccctaa tacgttctag gtataatcat tttgtcattc tttttactgt cgccataatg       60

tttctaacag tttgtttccc cgcttataaa gctttagcag attctacctc gacagaagtc      120

attgtgcatt atcatcgttt tgattctaac tatgcaaatt gggatctatg gatgtggcca      180

tatcaaccag ttaatggtaa tggagcagca tacgagtttt ctggaaagga tgattttggc      240

gttaaagcag atgttcaagt gcctggggat gatacacagg taggtctgat tgtccgtaca      300

aatgattgga gccaaaaaaa tacatcagac gatctccata ttgatctgac aaaggggcat      360

gaaatatgga ttgttcaggg ggatcccaat atttattaca atctgagtga tgcgcaggct      420

gcagcgactc caaaggtttc gaatgcgtat ttggataatg aaaaaacagt attggcaaag      480

ctaactaatc caatgacatt atcagatgga tcaagcggct ttacggttac agataaaaca      540

acaggggaac aaattccagt taccgctgca acaaatgcga actcagcctc ctcgtctgag      600

cagacagact tggttcaatt gacgttagcc agtgcaccgg atgtttccca tacaatacaa      660

gtaggagcag ccggttatga agcagtcaat ctcataccac gaaatgtatt aaatttgcct      720

cgttattatt acagcggaaa tgatttaggt aacgtttatt caaataaggc aacggccttc      780

cgtgtatggg ctccaactgc ttcggatgtc caattacttt tatacaatag tgaaacagga      840

cctgtaacca aacagcttga aatgcaaaag agtgataacg gtacatggaa actgaaggtc      900

cctggtaatc tgaaaaattg gtattatctc tatcaggtaa cggtgaatgg gaagacacaa      960

acagccgttg acccttatgt gcgtgctatt tcagtcaatg caacacgtgg tatgatagtc     1020

gatttagaag atacgaatcc tcctggatgg aaagaagatc atcaacagac acctgcgaac     1080

ccagtggatg aagtaatcta cgaagtgcat gtgcgtgatt tttcgattga tgctaattca     1140

ggcatgaaaa ataaagggaa atatcttgcc tttacagaac atggcacaaa aggccctgat     1200

aacgtgaaaa cgggtattga tagtttgaag gaattaggaa tcaatgctgt tcaattacag     1260

ccgattgaag aatttaacag cattgatgaa acccaaccaa atatgtataa ctggggctat     1320

gacccaagaa actacaacgt ccctgaagga gcgtatgcaa ctacaccaga aggaacggct     1380

cgcattaccc agttaaagca actgattcaa agcattcata aagatcggat tgctatcaat     1440

atggatgtgg tctataatca tacctttgcc acgcaaatct ctgacttcga taaaattgta     1500

ccagaatatt attaccgtac ggatgatgca ggtaattata ccaacggatc aggtactgga     1560

aatgaaatcg cagccgaaag gccaatggtt caaaaattta ttattgattc ccttaagtat     1620

tgggtcaatg agtatcatat tgacggcttc cgttttgact taatggcgct gcttggaaaa     1680

gacacgatgt cgaaagctgc ctcggagctt catgctatta atccaggaat tgcactttac     1740

ggtgagccat ggacgggtgg aacctctgca ctgccagaag atcagcttct gacaaaagga     1800

gctcaaaaag gcatgggagt agcggtgttt aatgacaatt tacgaaacgc gttggacggc     1860

aatgtctttg attcttccgc tcaaggtttt gcgacaggtg caacaggctt aactgatgca     1920

attaagaatg gcgttgaggg gagtattaat gactttacct cttcaccagg tgagacaatt     1980

aactatgtca caagtcatga taactacacc ctttgggaca aaatagccct aagcaaccct     2040

aatgattccg aagcggatcg gattaaaatg gatgaactcg cacaagcagt tgttatgacc     2100

tcacaaggtg ttccattcat gcaaggcggg gaagaaatgc ttcgtacaaa aggcggcaac     2160

gacaatagtt ataatgcagg cgatacggtc aatgagtttg attggagcag gaaagctcaa     2220

tatccagatg ttttcaacta ttatagcggg ctaatccacc ttcgtcttga tcacccagcc     2280

ttccgcatga cgacagctaa tgaaatcaat agccacctcc aattcctaaa tagtccagag     2340

aacacagtgg cctatgaatt aactgatcat gttaataaag acaaatgggg aaatatcatt     2400

gttgtttata acccaaataa aactgcagca accattaatt tgccgagcgg gaaatgggca     2460

atcaatgcta cgagcggtaa ggtaggagaa tccacccttg gtcaagcaga gggaagtgtc     2520

caagtaccag gtatatctat gatgatcctt catcaagagg taagcccaga ccacggtaaa     2580

aagtaa                                                                2586


<210>  5
<211>  515
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  5

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Arg Pro Ile Thr Thr 
                485                 490                 495     


Arg Pro Trp Thr Gly Glu Phe Val Arg Trp Thr Glu Pro Arg Leu Val 
            500                 505                 510         


Ala Trp Pro 
        515 


<210>  6
<211>  177
<212>  PRT
<213>  Thermoascus aurantiacus

<400>  6

Thr Arg Ile Ser Ser Cys Ser Gly Ser Arg Gln Ser Ala Leu Thr Thr 
1               5                   10                  15      


Ala Leu Arg Asn Ala Ala Ser Leu Ala Asn Ala Ala Ala Asp Ala Ala 
            20                  25                  30          


Gln Ser Gly Ser Ala Ser Lys Phe Ser Glu Tyr Phe Lys Thr Thr Ser 
        35                  40                  45              


Ser Ser Thr Arg Gln Thr Val Ala Ala Arg Leu Arg Ala Val Ala Arg 
    50                  55                  60                  


Glu Ala Ser Ser Ser Ser Ser Gly Ala Thr Thr Tyr Tyr Cys Asp Asp 
65                  70                  75                  80  


Pro Tyr Gly Tyr Cys Ser Ser Asn Val Leu Ala Tyr Thr Leu Pro Ser 
                85                  90                  95      


Tyr Asn Ile Ile Ala Asn Cys Asp Ile Phe Tyr Thr Tyr Leu Pro Ala 
            100                 105                 110         


Leu Thr Ser Thr Cys His Ala Gln Asp Gln Ala Thr Thr Ala Leu His 
        115                 120                 125             


Glu Phe Thr His Ala Pro Gly Val Tyr Ser Pro Gly Thr Asp Asp Leu 
    130                 135                 140                 


Ala Tyr Gly Tyr Gln Ala Ala Met Gly Leu Ser Ser Ser Gln Ala Val 
145                 150                 155                 160 


Met Asn Ala Asp Thr Tyr Ala Leu Tyr Ala Asn Ala Ile Tyr Leu Gly 
                165                 170                 175     


Cys 
    


<210>  7
<211>  412
<212>  PRT
<213>  Pyrococcus furiosus

<400>  7

Ala Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr 
1               5                   10                  15      


Tyr Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile 
            20                  25                  30          


Ile Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val 
        35                  40                  45              


Ile Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp 
    50                  55                  60                  


His Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala 
65                  70                  75                  80  


Ala Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala 
                85                  90                  95      


Gly Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile 
            100                 105                 110         


Ile Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile 
        115                 120                 125             


Lys Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr 
    130                 135                 140                 


Asp Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val 
145                 150                 155                 160 


Val Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly 
                165                 170                 175     


Ser Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys 
            180                 185                 190         


Tyr Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly 
        195                 200                 205             


Arg Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala 
    210                 215                 220                 


Arg Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr 
225                 230                 235                 240 


Ala Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala 
                245                 250                 255     


Ala Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys 
            260                 265                 270         


Thr Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala 
        275                 280                 285             


Asp Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn 
    290                 295                 300                 


Tyr Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys 
305                 310                 315                 320 


Gly Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr 
                325                 330                 335     


Ala Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu 
            340                 345                 350         


Tyr Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr 
        355                 360                 365             


Asp Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr 
    370                 375                 380                 


Ile Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val 
385                 390                 395                 400 


Val Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser 
                405                 410         


<210>  8
<211>  425
<212>  PRT
<213>  Thermococcus sp. PK

<400>  8

Met Glu Phe Asn Lys Val Phe Ser Leu Leu Leu Val Phe Val Val Leu 
1               5                   10                  15      


Gly Ala Thr Ala Gly Ile Val Gly Ala Ala Pro Ala Glu Lys Ala Arg 
            20                  25                  30          


Val Ile Ile Thr Ile Asp Lys Asp Phe Asn Glu Asn Ser Val Phe Ala 
        35                  40                  45              


Leu Gly Gly Asn Val Val Ala Arg Gly Lys Val Phe Pro Ile Val Ile 
    50                  55                  60                  


Ala Glu Leu Pro Pro Arg Ala Ile Glu Arg Leu Lys Asn Ala Lys Gly 
65                  70                  75                  80  


Val Val Arg Val Glu Tyr Asp Ala Glu Ala His Ile Leu Lys Gly Lys 
                85                  90                  95      


Pro Pro Gly Thr Gly Lys Pro Lys Pro Ser Gln Pro Ala Gln Thr Ile 
            100                 105                 110         


Pro Trp Gly Ile Glu Arg Ile Lys Ala Pro Asp Ala Trp Ser Ile Thr 
        115                 120                 125             


Asp Gly Ser Ser Gly Gly Val Ile Glu Val Ala Ile Leu Asp Thr Gly 
    130                 135                 140                 


Ile Asp Tyr Asp His Pro Asp Leu Ala Ala Asn Leu Ala Trp Gly Val 
145                 150                 155                 160 


Ser Val Leu Arg Gly Lys Val Ser Thr Asn Pro Lys Asp Tyr Lys Asp 
                165                 170                 175     


Gln Asn Gly His Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn 
            180                 185                 190         


Asn Asp Ile Gly Val Val Gly Val Ala Ser Ala Val Glu Ile Tyr Ala 
        195                 200                 205             


Val Arg Val Leu Asp Ala Ser Gly Arg Gly Ser Tyr Ser Asp Ile Ile 
    210                 215                 220                 


Leu Gly Ile Glu Gln Ala Leu Leu Gly Pro Asp Gly Val Leu Asp Ser 
225                 230                 235                 240 


Asp Asn Asp Gly Val Ile Val Gly Asp Pro Asp Asp Asp Ala Ala Glu 
                245                 250                 255     


Val Ile Ser Met Ser Leu Gly Gly Ser Ser Asp Val Gln Ala Phe His 
            260                 265                 270         


Asp Ala Ile Ile Glu Ala Tyr Asn Tyr Gly Val Val Ile Val Ala Ala 
        275                 280                 285             


Ser Gly Asn Asp Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro 
    290                 295                 300                 


Glu Val Ile Ala Val Gly Ala Thr Asp Ser Asp Asp Gln Val Pro Trp 
305                 310                 315                 320 


Trp Ser Asn Arg Gly Val Glu Val Ser Ala Pro Gly Val Asp Ile Leu 
                325                 330                 335     


Ser Thr Tyr Pro Asp Asp Thr Tyr Glu Thr Leu Ser Gly Thr Ser Met 
            340                 345                 350         


Ala Thr Pro His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala Tyr 
        355                 360                 365             


Tyr Asn Lys Tyr Gly Tyr Val Leu Pro Val Gly Thr Phe Gly Asp Leu 
    370                 375                 380                 


Thr Thr Ser Thr Val Arg Gly Ile Leu His Val Thr Ala Asp Asp Leu 
385                 390                 395                 400 


Gly Ser Ser Gly Trp Asp Ala Asp Tyr Gly Tyr Gly Ile Val Arg Ala 
                405                 410                 415     


Asp Leu Ala Val Gln Ala Ala Ile Ser 
            420                 425 


<210>  9
<211>  591
<212>  PRT
<213>  Talaromyces emersonii

<400>  9

Ala Thr Gly Ser Leu Asp Ser Phe Leu Ala Thr Glu Thr Pro Ile Ala 
1               5                   10                  15      


Leu Gln Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ala Asp Val Ala 
            20                  25                  30          


Gly Ala Ser Ala Gly Ile Val Val Ala Ser Pro Ser Arg Ser Asp Pro 
        35                  40                  45              


Asn Tyr Phe Tyr Ser Trp Thr Arg Asp Ala Ala Leu Thr Ala Lys Tyr 
    50                  55                  60                  


Leu Val Asp Ala Phe Ile Ala Gly Asn Lys Asp Leu Glu Gln Thr Ile 
65                  70                  75                  80  


Gln Gln Tyr Ile Ser Ala Gln Ala Lys Val Gln Thr Ile Ser Asn Pro 
                85                  90                  95      


Ser Gly Asp Leu Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn Val 
            100                 105                 110         


Asn Glu Thr Ala Phe Thr Gly Pro Trp Gly Arg Pro Gln Arg Asp Gly 
        115                 120                 125             


Pro Ala Leu Arg Ala Thr Ala Leu Ile Ala Tyr Ala Asn Tyr Leu Ile 
    130                 135                 140                 


Asp Asn Gly Glu Ala Ser Thr Ala Asp Glu Ile Ile Trp Pro Ile Val 
145                 150                 155                 160 


Gln Asn Asp Leu Ser Tyr Ile Thr Gln Tyr Trp Asn Ser Ser Thr Phe 
                165                 170                 175     


Asp Leu Trp Glu Glu Val Glu Gly Ser Ser Phe Phe Thr Thr Ala Val 
            180                 185                 190         


Gln His Arg Ala Leu Val Glu Gly Asn Ala Leu Ala Thr Arg Leu Asn 
        195                 200                 205             


His Thr Cys Ser Asn Cys Val Ser Gln Ala Pro Gln Val Leu Cys Phe 
    210                 215                 220                 


Leu Gln Ser Tyr Trp Thr Gly Ser Tyr Val Leu Ala Asn Phe Gly Gly 
225                 230                 235                 240 


Ser Gly Arg Ser Gly Lys Asp Val Asn Ser Ile Leu Gly Ser Ile His 
                245                 250                 255     


Thr Phe Asp Pro Ala Gly Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys 
            260                 265                 270         


Ser Ala Arg Ala Leu Ala Asn His Lys Val Val Thr Asp Ser Phe Arg 
        275                 280                 285             


Ser Ile Tyr Ala Ile Asn Ser Gly Ile Ala Glu Gly Ser Ala Val Ala 
    290                 295                 300                 


Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr 
305                 310                 315                 320 


Leu Ala Thr Ala Ala Ala Ala Glu Gln Leu Tyr Asp Ala Ile Tyr Gln 
                325                 330                 335     


Trp Lys Lys Ile Gly Ser Ile Ser Ile Thr Asp Val Ser Leu Pro Phe 
            340                 345                 350         


Phe Gln Asp Ile Tyr Pro Ser Ala Ala Val Gly Thr Tyr Asn Ser Gly 
        355                 360                 365             


Ser Thr Thr Phe Asn Asp Ile Ile Ser Ala Val Gln Thr Tyr Gly Asp 
    370                 375                 380                 


Gly Tyr Leu Ser Ile Val Glu Lys Tyr Thr Pro Ser Asp Gly Ser Leu 
385                 390                 395                 400 


Thr Glu Gln Phe Ser Arg Thr Asp Gly Thr Pro Leu Ser Ala Ser Ala 
                405                 410                 415     


Leu Thr Trp Ser Tyr Ala Ser Leu Leu Thr Ala Ser Ala Arg Arg Gln 
            420                 425                 430         


Ser Val Val Pro Ala Ser Trp Gly Glu Ser Ser Ala Ser Ser Val Pro 
        435                 440                 445             


Ala Val Cys Ser Ala Thr Ser Ala Thr Gly Pro Tyr Ser Thr Ala Thr 
    450                 455                 460                 


Asn Thr Val Trp Pro Ser Ser Gly Ser Gly Ser Ser Thr Thr Thr Ser 
465                 470                 475                 480 


Ser Ala Pro Cys Thr Thr Pro Thr Ser Val Ala Val Thr Phe Asp Glu 
                485                 490                 495     


Ile Val Ser Thr Ser Tyr Gly Glu Thr Ile Tyr Leu Ala Gly Ser Ile 
            500                 505                 510         


Pro Glu Leu Gly Asn Trp Ser Thr Ala Ser Ala Ile Pro Leu Arg Ala 
        515                 520                 525             


Asp Ala Tyr Thr Asn Ser Asn Pro Leu Trp Tyr Val Thr Val Asn Leu 
    530                 535                 540                 


Pro Pro Gly Thr Ser Phe Glu Tyr Lys Phe Phe Lys Asn Gln Thr Asp 
545                 550                 555                 560 


Gly Thr Ile Val Trp Glu Asp Asp Pro Asn Arg Ser Tyr Thr Val Pro 
                565                 570                 575     


Ala Tyr Cys Gly Gln Thr Thr Ala Ile Leu Asp Asp Ser Trp Gln 
            580                 585                 590     


<210>  10
<211>  555
<212>  PRT
<213>  Pycnoporus sanguineus

<400>  10

Gln Ser Ser Ala Val Asp Ala Tyr Val Ala Ser Glu Ser Pro Ile Ala 
1               5                   10                  15      


Lys Gln Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ser Lys Ala His 
            20                  25                  30          


Gly Ala Lys Ala Gly Ile Val Val Ala Ser Pro Ser Thr Glu Asn Pro 
        35                  40                  45              


Asp Tyr Leu Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Leu 
    50                  55                  60                  


Leu Ile Asp Gln Phe Thr Ser Gly Asp Asp Thr Ser Leu Arg Gly Leu 
65                  70                  75                  80  


Ile Asp Asp Phe Thr Ser Ala Glu Ala Ile Leu Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Ile Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ala Thr Ser Ile Ile Arg Tyr Ala Asn Trp Leu 
    130                 135                 140                 


Leu Asp Asn Gly Asn Thr Thr Tyr Val Ser Asn Thr Leu Trp Pro Val 
145                 150                 155                 160 


Ile Gln Leu Asp Leu Asp Tyr Val Ala Asp Asn Trp Asn Gln Ser Thr 
                165                 170                 175     


Phe Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Thr Phe Ala Ser Arg Ile 
        195                 200                 205             


Gly Gln Ser Ser Val Val Ser Gly Tyr Thr Thr Gln Ala Asp Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Val Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ser Asn Thr Val Leu 
                245                 250                 255     


Thr Ser Ile His Thr Phe Asp Pro Ala Ala Gly Cys Asp Ala Ala Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ala Phe Arg Ser Ile Tyr Thr Ile Asn Asn Gly Ile Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Met Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Ser Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Tyr Val Trp Asp Gln Leu Gly Gly Leu Asn Val Thr Ser Thr 
            340                 345                 350         


Ser Leu Ala Phe Phe Gln Gln Phe Ala Ser Gly Leu Ser Thr Gly Thr 
        355                 360                 365             


Tyr Ser Ala Ser Ser Ser Thr Tyr Ala Thr Leu Thr Ser Ala Ile Arg 
    370                 375                 380                 


Ser Phe Ala Asp Gly Phe Leu Ala Ile Asn Ala Lys Tyr Thr Pro Ala 
385                 390                 395                 400 


Asp Gly Gly Leu Ala Glu Gln Tyr Ser Arg Asn Asp Gly Thr Pro Leu 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ala Phe 
            420                 425                 430         


Ala Ala Arg Glu Gly Lys Thr Tyr Gly Ser Trp Gly Ala Ala Gly Leu 
        435                 440                 445             


Thr Val Pro Ala Ser Cys Ser Gly Gly Gly Gly Ala Thr Val Ala Val 
    450                 455                 460                 


Thr Phe Asn Val Gln Ala Thr Thr Val Phe Gly Glu Asn Ile Tyr Ile 
465                 470                 475                 480 


Thr Gly Ser Val Ala Ala Leu Gln Asn Trp Ser Pro Asp Asn Ala Leu 
                485                 490                 495     


Ile Leu Ser Ala Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val Asn Leu 
            500                 505                 510         


Pro Ala Asn Thr Val Val Gln Tyr Lys Tyr Ile Arg Lys Phe Asn Gly 
        515                 520                 525             


Gln Val Thr Trp Glu Ser Asp Pro Asn Asn Gln Ile Thr Thr Pro Ser 
    530                 535                 540                 


Gly Gly Ser Phe Thr Gln Asn Asp Val Trp Arg 
545                 550                 555 


<210>  11
<211>  556
<212>  PRT
<213>  Gloeophyllum sepiarum

<400>  11

Gln Ser Val Asp Ser Tyr Val Ser Ser Glu Gly Pro Ile Ala Lys Ala 
1               5                   10                  15      


Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly Ala 
            20                  25                  30          


Ser Ala Gly Val Val Val Ala Ser Pro Ser Thr Ser Asp Pro Asp Tyr 
        35                  40                  45              


Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu Ile 
    50                  55                  60                  


Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Thr Leu 
65                  70                  75                  80  


Ile Asp Asp Phe Val Thr Ala Glu Ala Asn Leu Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Gly Asn Trp Leu 
    130                 135                 140                 


Leu Ser Asn Gly Asn Thr Ser Tyr Val Thr Ser Asn Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Gln Asn Asp Leu Gly Tyr Val Val Ser Tyr Trp Asn Gln Ser Thr 
                165                 170                 175     


Tyr Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Ala Ile 
        195                 200                 205             


Gly Gln Thr Ser Gln Val Ser Ser Tyr Thr Thr Gln Ala Asp Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Ala Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Val Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Asn Val Trp Glu Ser Gln Gly Ser Leu Glu Val Thr Ser Thr 
            340                 345                 350         


Ser Leu Ala Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Ala Gly Thr 
        355                 360                 365             


Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Asn Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Lys Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Tyr Ser Lys Ser Asp Gly Ser Pro Leu 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala Phe 
            420                 425                 430         


Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Ala Gly Leu 
        435                 440                 445             


Thr Val Pro Ser Ser Cys Ser Gly Asn Ser Gly Gly Pro Thr Val Ala 
    450                 455                 460                 


Val Thr Phe Asn Val Asn Ala Glu Thr Val Trp Gly Glu Asn Ile Tyr 
465                 470                 475                 480 


Leu Thr Gly Ser Val Asp Ala Leu Glu Asn Trp Ser Ala Asp Asn Ala 
                485                 490                 495     


Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val Asn 
            500                 505                 510         


Leu Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys Asn Asn 
        515                 520                 525             


Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr Pro 
    530                 535                 540                 


Ala Ser Gly Ser Thr Thr Glu Asn Asp Thr Trp Arg 
545                 550                 555     


<210>  12
<211>  559
<212>  PRT
<213>  Gloeophyllum trabeum

<400>  12

Gln Ser Val Asp Ser Tyr Val Gly Ser Glu Gly Pro Ile Ala Lys Ala 
1               5                   10                  15      


Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly Ala 
            20                  25                  30          


Ala Ala Gly Val Val Val Ala Ser Pro Ser Lys Ser Asp Pro Asp Tyr 
        35                  40                  45              


Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu Ile 
    50                  55                  60                  


Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Ser Leu 
65                  70                  75                  80  


Ile Asp Ser Phe Val Ile Ala Glu Ala Asn Ile Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ala Thr Ala Leu Ile Thr Tyr Gly Asn Trp Leu 
    130                 135                 140                 


Leu Ser Asn Gly Asn Thr Thr Trp Val Thr Ser Thr Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Gln Asn Asp Leu Asn Tyr Val Val Gln Tyr Trp Asn Gln Thr Thr 
                165                 170                 175     


Phe Asp Leu Trp Glu Glu Val Asn Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Lys Ile 
        195                 200                 205             


Gly Gln Thr Ser Ser Val Ser Ser Tyr Thr Thr Gln Ala Ala Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Thr Ser Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Thr Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Ile Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Asn Val Trp Ala Ala Gln Gly Ser Leu Asn Val Thr Ser Ile 
            340                 345                 350         


Ser Leu Pro Phe Phe Gln Gln Phe Ser Ser Ser Val Thr Ala Gly Thr 
        355                 360                 365             


Tyr Ala Ser Ser Ser Thr Thr Tyr Thr Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Ser Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Gln Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Phe Ser Arg Ser Asn Gly Ala Pro Val 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala Phe 
            420                 425                 430         


Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Val Gly Leu 
        435                 440                 445             


Thr Val Pro Thr Ser Cys Ser Ser Asn Ser Gly Gly Gly Gly Gly Ser 
    450                 455                 460                 


Thr Val Ala Val Thr Phe Asn Val Asn Ala Gln Thr Val Trp Gly Glu 
465                 470                 475                 480 


Asn Ile Tyr Ile Thr Gly Ser Val Asp Ala Leu Ser Asn Trp Ser Pro 
                485                 490                 495     


Asp Asn Ala Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile 
            500                 505                 510         


Thr Val Asn Leu Pro Ala Ser Thr Ala Ile Gln Tyr Lys Tyr Ile Arg 
        515                 520                 525             


Lys Asn Asn Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile 
    530                 535                 540                 


Thr Thr Pro Ala Ser Gly Ser Val Thr Glu Asn Asp Thr Trp Arg 
545                 550                 555                 


<210>  13
<211>  556
<212>  PRT
<213>  Trametes cingulata

<400>  13

Gln Ser Ser Ala Ala Asp Ala Tyr Val Ala Ser Glu Ser Pro Ile Ala 
1               5                   10                  15      


Lys Ala Gly Val Leu Ala Asn Ile Gly Pro Ser Gly Ser Lys Ser Asn 
            20                  25                  30          


Gly Ala Lys Ala Gly Ile Val Ile Ala Ser Pro Ser Thr Ser Asn Pro 
        35                  40                  45              


Asn Tyr Leu Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ala 
    50                  55                  60                  


Leu Ile Asp Gln Phe Thr Thr Gly Glu Asp Thr Ser Leu Arg Thr Leu 
65                  70                  75                  80  


Ile Asp Glu Phe Thr Ser Ala Glu Ala Ile Leu Gln Gln Val Pro Asn 
                85                  90                  95      


Pro Ser Gly Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Ile Asp Glu Thr Ala Phe Thr Asp Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ala Thr Ala Ile Ile Thr Tyr Ala Asn Trp Leu 
    130                 135                 140                 


Leu Asp Asn Lys Asn Thr Thr Tyr Val Thr Asn Thr Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Lys Leu Asp Leu Asp Tyr Val Ala Ser Asn Trp Asn Gln Ser Thr 
                165                 170                 175     


Phe Asp Leu Trp Glu Glu Ile Asn Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Thr Phe Ala Asn Arg Ile 
        195                 200                 205             


Gly Gln Thr Ser Val Val Ser Gly Tyr Thr Thr Gln Ala Asn Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Thr Gly Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Val Leu 
                245                 250                 255     


Thr Ser Ile His Thr Phe Asp Pro Ala Ala Gly Cys Asp Ala Val Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ala Phe Arg Ser Ile Tyr Ser Ile Asn Ser Gly Ile Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Met Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Ser Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Ile Val Trp Asn Lys Leu Gly Ala Leu Asn Val Thr Ser Thr 
            340                 345                 350         


Ser Leu Pro Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Val Gly Thr 
        355                 360                 365             


Tyr Ala Ser Ser Ser Ser Thr Phe Lys Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Thr Phe Ala Asp Gly Phe Leu Ala Val Asn Ala Lys Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Tyr Ser Arg Ser Asn Gly Ser Pro Val 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ser Phe 
            420                 425                 430         


Ala Ala Arg Ser Gly Lys Thr Tyr Ala Ser Trp Gly Ala Ala Gly Leu 
        435                 440                 445             


Thr Val Pro Thr Thr Cys Ser Gly Ser Gly Gly Ala Gly Thr Val Ala 
    450                 455                 460                 


Val Thr Phe Asn Val Gln Ala Thr Thr Val Phe Gly Glu Asn Ile Tyr 
465                 470                 475                 480 


Ile Thr Gly Ser Val Pro Ala Leu Gln Asn Trp Ser Pro Asp Asn Ala 
                485                 490                 495     


Leu Ile Leu Ser Ala Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val Asn 
            500                 505                 510         


Leu Pro Ala Ser Thr Thr Ile Glu Tyr Lys Tyr Ile Arg Lys Phe Asn 
        515                 520                 525             


Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr Pro 
    530                 535                 540                 


Ala Ser Gly Thr Phe Thr Gln Asn Asp Thr Trp Arg 
545                 550                 555     


<210>  14
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid alpha-amylase

<400>  14

Ala Thr Ser Asp Asp Trp Lys Gly Lys Ala Ile Tyr Gln Leu Leu Thr 
1               5                   10                  15      


Asp Arg Phe Gly Arg Ala Asp Asp Ser Thr Ser Asn Cys Ser Asn Leu 
            20                  25                  30          


Ser Asn Tyr Cys Gly Gly Thr Tyr Glu Gly Ile Thr Lys His Leu Asp 
        35                  40                  45              


Tyr Ile Ser Gly Met Gly Phe Asp Ala Ile Trp Ile Ser Pro Ile Pro 
    50                  55                  60                  


Lys Asn Ser Asp Gly Gly Tyr His Gly Tyr Trp Ala Thr Asp Phe Tyr 
65                  70                  75                  80  


Gln Leu Asn Ser Asn Phe Gly Asp Glu Ser Gln Leu Lys Ala Leu Ile 
                85                  90                  95      


Gln Ala Ala His Glu Arg Asp Met Tyr Val Met Leu Asp Val Val Ala 
            100                 105                 110         


Asn His Ala Gly Pro Thr Ser Asn Gly Tyr Ser Gly Tyr Thr Phe Gly 
        115                 120                 125             


Asp Ala Ser Leu Tyr His Pro Lys Cys Thr Ile Asp Tyr Asn Asp Gln 
    130                 135                 140                 


Thr Ser Ile Glu Gln Cys Trp Val Ala Asp Glu Leu Pro Asp Ile Asp 
145                 150                 155                 160 


Thr Glu Asn Ser Asp Asn Val Ala Ile Leu Asn Asp Ile Val Ser Gly 
                165                 170                 175     


Trp Val Gly Asn Tyr Ser Phe Asp Gly Ile Arg Ile Asp Thr Val Lys 
            180                 185                 190         


His Ile Arg Lys Asp Phe Trp Thr Gly Tyr Ala Glu Ala Ala Gly Val 
        195                 200                 205             


Phe Ala Thr Gly Glu Val Phe Asn Gly Asp Pro Ala Tyr Val Gly Pro 
    210                 215                 220                 


Tyr Gln Lys Tyr Leu Pro Ser Leu Ile Asn Tyr Pro Met Tyr Tyr Ala 
225                 230                 235                 240 


Leu Asn Asp Val Phe Val Ser Lys Ser Lys Gly Phe Ser Arg Ile Ser 
                245                 250                 255     


Glu Met Leu Gly Ser Asn Arg Asn Ala Phe Glu Asp Thr Ser Val Leu 
            260                 265                 270         


Thr Thr Phe Val Asp Asn His Asp Asn Pro Arg Phe Leu Asn Ser Gln 
        275                 280                 285             


Ser Asp Lys Ala Leu Phe Lys Asn Ala Leu Thr Tyr Val Leu Leu Gly 
    290                 295                 300                 


Glu Gly Ile Pro Ile Val Tyr Tyr Gly Ser Glu Gln Gly Phe Ser Gly 
305                 310                 315                 320 


Gly Ala Asp Pro Ala Asn Arg Glu Val Leu Trp Thr Thr Asn Tyr Asp 
                325                 330                 335     


Thr Ser Ser Asp Leu Tyr Gln Phe Ile Lys Thr Val Asn Ser Val Arg 
            340                 345                 350         


Met Lys Ser Asn Lys Ala Val Tyr Met Asp Ile Tyr Val Gly Asp Asn 
        355                 360                 365             


Ala Tyr Ala Phe Lys His Gly Asp Ala Leu Val Val Leu Asn Asn Tyr 
    370                 375                 380                 


Gly Ser Gly Ser Thr Asn Gln Val Ser Phe Ser Val Ser Gly Lys Phe 
385                 390                 395                 400 


Asp Ser Gly Ala Ser Leu Met Asp Ile Val Ser Asn Ile Thr Thr Thr 
                405                 410                 415     


Val Ser Ser Asp Gly Thr Val Thr Phe Asn Leu Lys Asp Gly Leu Pro 
            420                 425                 430         


Ala Ile Phe Thr Ser Ala Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro 
        435                 440                 445             


Thr Gly Ser Gly Ser Val Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala 
    450                 455                 460                 


Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr Ser Cys Thr Thr Pro Thr 
465                 470                 475                 480 


Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr Thr Thr Tyr Gly Glu 
                485                 490                 495     


Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu Gly Asp Trp Glu Thr 
            500                 505                 510         


Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr Thr Ser Ser Asp Pro 
        515                 520                 525             


Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Glu Ser Phe Glu Tyr 
    530                 535                 540                 


Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val Glu Trp Glu Ser Asp 
545                 550                 555                 560 


Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys Gly Thr Ser Thr Ala 
                565                 570                 575     


Thr Val Thr Asp Thr Trp Arg 
            580             


<210>  15
<211>  828
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid variant pullulanase

<400>  15

Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp Ser 
1               5                   10                  15      


Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val Asn 
            20                  25                  30          


Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly Val 
        35                  40                  45              


Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu Ile 
    50                  55                  60                  


Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu His 
65                  70                  75                  80  


Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp Pro 
                85                  90                  95      


Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro Lys 
            100                 105                 110         


Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys Leu 
        115                 120                 125             


Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val Thr 
    130                 135                 140                 


Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn Ala 
145                 150                 155                 160 


Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr Leu 
                165                 170                 175     


Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala Gly 
            180                 185                 190         


Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro Arg 
        195                 200                 205             


Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Pro Lys Ala 
    210                 215                 220                 


Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu Leu 
225                 230                 235                 240 


Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Ala Leu Glu Met Arg 
                245                 250                 255     


Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu Lys 
            260                 265                 270         


Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln Thr 
        275                 280                 285             


Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg Gly 
    290                 295                 300                 


Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu Asp 
305                 310                 315                 320 


His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu Val 
                325                 330                 335     


His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn Lys 
            340                 345                 350         


Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp Gly 
        355                 360                 365             


Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala Val 
    370                 375                 380                 


Gln Leu Gln Pro Ile Glu Glu Phe Ala Ser Ile Asp Glu Thr Gln Pro 
385                 390                 395                 400 


Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro Glu 
                405                 410                 415     


Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Glu Phe 
            420                 425                 430         


Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn Met 
        435                 440                 445             


Asp Val Val Tyr Asn His Thr Phe Ala Thr Gln Ile Ser Asp Phe Asp 
    450                 455                 460                 


Lys Ile Val Pro Glu Tyr Tyr Tyr Arg Thr Asp Asp Ala Gly Asn Tyr 
465                 470                 475                 480 


Thr Asn Gly Ser Gly Thr Gly Asn Glu Ile Ala Ala Glu Arg Pro Met 
                485                 490                 495     


Val Gln Lys Phe Ile Ile Asp Ser Leu Lys Tyr Trp Val Asn Glu Tyr 
            500                 505                 510         


His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys Asp 
        515                 520                 525             


Thr Met Ser Lys Ala Ala Ser Glu Leu His Ala Ile Asn Pro Gly Ile 
    530                 535                 540                 


Ala Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Ala Leu Pro Glu 
545                 550                 555                 560 


Asp Gln Leu Leu Thr Lys Gly Ala Gln Lys Gly Met Gly Val Ala Val 
                565                 570                 575     


Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn Val Phe Asp Ser 
            580                 585                 590         


Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala Ile 
        595                 600                 605             


Lys Arg Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro Ser 
    610                 615                 620                 


Glu Thr Ile Asn Tyr Val Ser Cys His Asp Asn Tyr Thr Leu Trp Asp 
625                 630                 635                 640 


Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile Lys 
                645                 650                 655     


Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val Pro 
            660                 665                 670         


Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn Asp 
        675                 680                 685             


Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe Asp Trp Ser Arg 
    690                 695                 700                 


Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile His 
705                 710                 715                 720 


Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu Ile 
                725                 730                 735     


Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala Tyr 
            740                 745                 750         


Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile Val 
        755                 760                 765             


Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser Gly 
    770                 775                 780                 


Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Ser Thr Leu 
785                 790                 795                 800 


Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met Ile 
                805                 810                 815     


Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
            820                 825             


<210>  16
<211>  2586
<212>  DNA
<213>  Artificial

<220>
<223>  CDS for P380-2

<400>  16
atgtccctaa tacgttctag gtataatcat tttgtcattc tttttactgt cgccataatg       60

tttctaacag tttgtttccc cgcttataaa gctttagcag attctacctc gacagaagtc      120

attgtgcatt atcatcgttt tgattctaac tatgcaaatt gggatctatg gatgtggcca      180

tatcaaccag ttaatggtaa tggagcagca tacgagtttt ctggaaagga tgattttggc      240

gttaaagcag atgttcaagt gcctggggat gatacacagg taggtctgat tgtccgtaca      300

aatgattgga gccaaaaaaa tacatcagac gatctccata ttgatctgac aaaggggcat      360

gaaatatgga ttgttcaggg ggatcccaat atttattaca atctgagtga tgcgcaggct      420

gcagcgactc caaaggtttc gaatgcgtat ttggataatg aaaaaacagt attggcaaag      480

ctaactaatc caatgacatt atcagatgga tcaagcggct ttacggttac agataaaaca      540

acaggggaac aaattccagt taccgctgca acaaatgcga actcagcctc ctcgtctgag      600

cagacagact tggttcaatt gacgttagcc agtgcaccgg atgtttccca tacaatacaa      660

gtaggagcag ccggttatga agcagtcaat ctcataccac gaaatgtatt aaatttgcct      720

cgttattatt acagcggaaa tgatttaggt aacgtttatt caccgaaggc aacggccttc      780

cgtgtatggg ctccaactgc ttcggatgtc caattacttt tatacaatag tgaaacagga      840

cctgtaacca aagcacttga aatgcgcaag agtgataacg gtacatggaa actgaaggtc      900

cctggtaatc tgaaaaattg gtattatctc tatcaggtaa cggtgaatgg gaagacacaa      960

acagccgttg acccttatgt gcgtgctatt tcagtcaatg caacacgtgg tatgatagtc     1020

gatttagaag atacgaatcc tcctggatgg aaagaagatc atcaacagac acctgcgaac     1080

ccagtggatg aagtaatcta cgaagtgcat gtgcgtgatt tttcgattga tgctaattca     1140

ggcatgaaaa ataaagggaa atatcttgcc tttacagaac atggcacaaa aggccctgat     1200

ggcgtgaaaa cgggtattga tagtttgaag gaattaggaa tcaatgctgt tcaattacag     1260

ccgattgaag aatttgccag cattgatgaa acccaaccaa atatgtataa ctggggctat     1320

gacccaagaa actacaacgt ccctgaagga gcgtatgcaa ctacaccaga aggaacggct     1380

cgcattacgg agtttaagca actgattcaa agcattcata aagatcggat tgctatcaat     1440

atggatgtgg tctataatca tacctttgcc acgcaaatct ctgacttcga taaaattgta     1500

ccagaatatt attaccgtac ggatgatgca ggtaattata ccaacggatc aggtactgga     1560

aatgaaatcg cagccgaaag gccaatggtt caaaaattta ttattgattc ccttaagtat     1620

tgggtcaatg agtatcatat tgacggcttc cgttttgact taatggcgct gcttggaaaa     1680

gacacgatgt cgaaagctgc ctcggagctt catgctatta atccaggaat tgcactttac     1740

ggtgagccat ggacgggtgg aacctctgca ctgccagaag atcagcttct gacaaaagga     1800

gctcaaaaag gcatgggagt agcggtgttt aatgacaatt tacgaaacgc gttggacggc     1860

aatgtctttg attcttccgc tcaaggtttt gcgacaggtg caacaggctt aactgatgca     1920

attaagcgcg gcgttgaggg gagtattaat gactttacct cttcaccaag cgagacaatt     1980

aactatgtct catgtcatga taactacacc ctttgggaca aaatagccct aagcaaccct     2040

aatgattccg aagcggatcg gattaaaatg gatgaactcg cacaagcagt tgttatgacc     2100

tcacaaggtg ttccattcat gcaaggcggg gaagaaatgc ttcgtacaaa aggcggcaac     2160

gacaatagtt ataatgcagg cgatacggtc aatgagtttg attggagcag gaaagctcaa     2220

tatccagatg ttttcaacta ttatagcggg ctaatccacc ttcgtcttga tcacccagcc     2280

ttccgcatga cgacagctaa tgaaatcaat agccacctcc aattcctaaa tagtccagag     2340

aacacagtgg cctatgaatt aactgatcat gttaataaag acaaatgggg aaatatcatt     2400

gttgtttata acccaaataa aactgcagca accattaatt tgccgagcgg gaaatgggca     2460

atcaatgcta cgagcggtaa ggtaggagaa tccacccttg gtcaagcaga gggaagtgtt     2520

caagtcccag gtatatctat gatgatcctt catcaagagg taagcccaga ccacggtaaa     2580

aagtaa                                                                2586


<210>  17
<211>  828
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid variant pullulanase

<400>  17

Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp Ser 
1               5                   10                  15      


Asn Tyr Ala Gly Trp Asp Leu Trp Met Trp Pro Lys Gln Pro Val Asn 
            20                  25                  30          


Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly Val 
        35                  40                  45              


Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu Ile 
    50                  55                  60                  


Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu Tyr 
65                  70                  75                  80  


Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp Pro 
                85                  90                  95      


Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro Lys 
            100                 105                 110         


Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys Leu 
        115                 120                 125             


Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val Thr 
    130                 135                 140                 


Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn Ala 
145                 150                 155                 160 


Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr Leu 
                165                 170                 175     


Ala Ser Ala Pro Asp Val Ser His Thr Ile Arg Val Gly Ala Ala Gly 
            180                 185                 190         


Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro Arg 
        195                 200                 205             


Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Pro Lys Ala 
    210                 215                 220                 


Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu Leu 
225                 230                 235                 240 


Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Ala Leu Glu Met Arg 
                245                 250                 255     


Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu Lys 
            260                 265                 270         


Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln Thr 
        275                 280                 285             


Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg Gly 
    290                 295                 300                 


Met Ile Val Asp Leu Ala Lys Thr Asn Pro Pro Gly Trp Lys Glu Asp 
305                 310                 315                 320 


His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu Val 
                325                 330                 335     


His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn Lys 
            340                 345                 350         


Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp Gly 
        355                 360                 365             


Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala Val 
    370                 375                 380                 


Gln Leu Leu Pro Ile Glu Glu Phe Ala Ser Ile Asp Glu Thr Gln Pro 
385                 390                 395                 400 


Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro Glu 
                405                 410                 415     


Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Glu Phe 
            420                 425                 430         


Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn Met 
        435                 440                 445             


Asp Val Val Tyr Asn His Thr Phe Ala Thr Gly Ile Ser Asp Phe Asp 
    450                 455                 460                 


Lys Ile Val Pro Glu Tyr Tyr Tyr Arg Thr Asp Asp Ala Gly Asn Tyr 
465                 470                 475                 480 


Thr Asn Gly Ser Gly Thr Gly Asn Glu Ile Ala Ala Glu Arg Pro Met 
                485                 490                 495     


Val Gln Lys Phe Ile Ile Asp Ser Leu Lys Tyr Trp Val Asn Glu Tyr 
            500                 505                 510         


His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys Asp 
        515                 520                 525             


Thr Met Ser Lys Ala Ala Ser Glu Leu His Ala Ile Asn Pro Gly Ile 
    530                 535                 540                 


Ala Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Ala Leu Pro Glu 
545                 550                 555                 560 


Asp Gln Leu Leu Thr Lys Gly Ala Gln Lys Gly Met Gly Val Ala Val 
                565                 570                 575     


Phe Asn Asp Asn Leu Arg Asn Ala Leu Ser Gly Asn Val Phe Asp Ser 
            580                 585                 590         


Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala Ile 
        595                 600                 605             


Lys Arg Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro Ser 
    610                 615                 620                 


Glu Thr Ile Asn Tyr Val Ser Cys His Asp Asn Tyr Thr Leu Trp Asp 
625                 630                 635                 640 


Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile Lys 
                645                 650                 655     


Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val Pro 
            660                 665                 670         


Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn Asp 
        675                 680                 685             


Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Arg Phe Asp Trp Ser Arg 
    690                 695                 700                 


Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile His 
705                 710                 715                 720 


Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu Ile 
                725                 730                 735     


Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala Tyr 
            740                 745                 750         


Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile Val 
        755                 760                 765             


Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser Gly 
    770                 775                 780                 


Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Arg Thr Leu 
785                 790                 795                 800 


Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met Ile 
                805                 810                 815     


Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
            820                 825             


<210>  18
<211>  2586
<212>  DNA
<213>  Artificial

<220>
<223>  CDS for P507-2

<400>  18
atgtccctaa tacgttctag gtataatcat tttgtcattc tttttactgt cgccataatg       60

tttctaacag tttgtttccc cgcttataaa gctttagcag attctacctc gacagaagtc      120

attgtgcatt atcatcgttt tgattctaac tatgcaggct gggatctatg gatgtggcca      180

aaacaaccag ttaatggtaa tggagcagca tacgagtttt ctggaaagga tgattttggc      240

gttaaagcag atgttcaagt gcctggggat gatacacagg taggtctgat tgtccgtaca      300

aatgattgga gccaaaaaaa tacatcagac gatctctata ttgatctgac aaaggggcat      360

gaaatatgga ttgttcaggg ggatcccaat atttattaca atctgagtga tgcgcaggct      420

gcagcgactc caaaggtttc gaatgcgtat ttggataatg aaaaaacagt attggcaaag      480

ctaactaatc caatgacatt atcagatgga tcaagcggct ttacggttac agataaaaca      540

acaggggaac aaattccagt taccgctgca acaaatgcga actcagcctc ctcgtctgag      600

cagacagact tggttcaatt gacgttagcc agtgcaccgg atgtttccca tacaataaga      660

gtaggagcag ccggttatga agcagtcaat ctcataccac gaaatgtatt aaatttgcct      720

cgttattatt acagcggaaa tgatttaggt aacgtttatt caccgaaggc aacggccttc      780

cgtgtatggg ctccaactgc ttcggatgtc caattacttt tatacaatag tgaaacagga      840

cctgtaacca aagcacttga aatgcgcaag agtgataacg gtacatggaa actgaaggtc      900

cctggtaatc tgaaaaattg gtattatctc tatcaggtaa cggtgaatgg gaagacacaa      960

acagccgttg acccttatgt gcgtgctatt tcagtcaatg caacacgtgg tatgatagtc     1020

gatttagcaa aaacgaatcc tcctggatgg aaagaagatc atcaacagac acctgcgaac     1080

ccagtggatg aagtaatcta cgaagtgcat gtgcgtgatt tttcgattga tgctaattca     1140

ggcatgaaaa ataaagggaa atatcttgcc tttacagaac atggcacaaa aggccctgat     1200

ggcgtgaaaa cgggtattga tagtttgaag gaattaggaa tcaatgctgt tcaattactg     1260

ccgattgaag aatttgccag cattgatgaa acccaaccaa atatgtataa ctggggctat     1320

gacccaagaa actacaacgt ccctgaagga gcgtatgcaa ctacaccaga aggaacggct     1380

cgcattacgg agtttaagca actgattcaa agcattcata aagatcggat tgctatcaat     1440

atggatgtgg tctataatca tacctttgcc acgggaatct ctgacttcga taaaattgta     1500

ccagaatatt attaccgtac ggatgatgca ggtaattata ccaacggatc aggtactgga     1560

aatgaaatcg cagccgaaag gccaatggtt caaaaattta ttattgattc ccttaagtat     1620

tgggtcaatg agtatcatat tgacggcttc cgttttgact taatggcgct gcttggaaaa     1680

gacacgatgt cgaaagctgc ctcggagctt catgctatta atccaggaat tgcactttac     1740

ggtgagccat ggacgggtgg aacctctgca ctgccagaag atcagcttct gacaaaagga     1800

gctcaaaaag gcatgggagt agcggtgttt aatgacaatt tacgaaacgc gttgtcaggc     1860

aatgtctttg attcttccgc tcaaggtttt gcgacaggtg caacaggctt aactgatgca     1920

attaagcgcg gcgttgaggg gagtattaat gactttacct cttcaccaag cgagacaatt     1980

aactatgtct catgtcatga taactacacc ctttgggaca aaatagccct aagcaaccct     2040

aatgattccg aagcggatcg gattaaaatg gatgaactcg cacaagcagt tgttatgacc     2100

tcacaaggtg ttccattcat gcaaggcggg gaagaaatgc ttcgtacaaa aggcggcaac     2160

gacaatagtt ataatgcagg cgatacggtc aatcggtttg attggagcag gaaagctcaa     2220

tatccagatg ttttcaacta ttatagcggg ctaatccacc ttcgtcttga tcacccagcc     2280

ttccgcatga cgacagctaa tgaaatcaat agccacctcc aattcctaaa tagtccagag     2340

aacacagtgg cctatgaatt aactgatcat gttaataaag acaaatgggg aaatatcatt     2400

gttgtttata acccaaataa aactgcagca accattaatt tgccgagcgg gaaatgggca     2460

atcaatgcta cgagcggtaa ggtaggagaa agaacccttg gtcaagcaga gggaagtgtc     2520

caagtaccag gtatatctat gatgatcctt catcaagagg taagcccaga ccacggtaaa     2580

aagtaa                                                                2586


<210>  19
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  19
atgtattatg gagctctata aaaatgagga gggaaccgaa tgtccctaat acgttctag        59


<210>  20
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  20
ttattgatta acgcgtttaa ttttgatcaa tgacatc                                37


<210>  21
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  21
atgtattatg gagctctata aaaatgagga gggaaccgaa tggctaaaaa actaatttat       60

g                                                                       61


<210>  22
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  22
ttattgatta acgcgtttac tttttaccgt ggtctg                                 36


