                         SEQUENCE LISTING

<110>  University of Saskatchewan
 
<120>  MYCOPLASMA VACCINES AND USES THEREOF

<130>  08935136WO

<140>  
<141>  2016-07-22

<150>  US 62/195,602
<151>  2015-07-22

<150>  US 62/195,581
<151>  2015-07-22

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  1092
<212>  DNA
<213>  Mycoplasma bovis

<400>  1
atgaattata aatatgttaa accaggtcac gctactagcg acccaaatga aacagttcgt       60

tttcttgatg ttgatggaaa attaattcaa gaatataaac catcagaaga aaccaagaaa      120

aaactagttg aaatgtataa aaatatgatt agatctcgtc aatgagactt atactcatta      180

acattacaaa aaactggtcg tttaggtact tttgctcctg cattaggtga agaagctgct      240

ttaacaggta ttggttacaa tcttaacaaa gaagactgat ttattccaca ctaccgtgta      300

ttaccaactc aattagcacg tggaatttct atggataaaa tttattcata ctgacaaggt      360

tctgaaattg gatcagcatt tgctggaaca aatacattgc cacttcacgt tgttattggt      420

tcacaagttc ctattgctgc tggtgttgca caagcgctta aattccaagg taaaaaagca      480

ttagctatgg ttacaattgg taatggtgga acaaacgaag gtgaattcca tgaaggatta      540

aacatggctt cagttcgtaa atgaccactt gttacagttg ttatgaacaa ccaatgagca      600

atttctgttc ctgaacacaa cagttacatt gttaagactc tttcacaaag agctaagtca      660

tatgatatgc caggtgttcg tgtagacggt aatgacttaa ttgctgttaa cgaagttatg      720

caagaagttt atgactttgt aagagaagga aatggacctg ttttagttga aatggttaca      780

tgacgtcaag gtcaacacac aactagtgac aaccctagag tttaccgttc tagagaactt      840

gaagttgaaa aagaaaaatg agaaccattc caccgtattg aagcttacct tctttcagaa      900

aaacttatta ctgaagaaga cattaaggtt tgatcagaag ctgcttatga agaagctaaa      960

gctgcatatg cactttcaaa agaattatgt gaagcaacaa cattcgatca catttacgac     1020

tacacatacg aaaaattacc tgcagattta gtaagacaaa aagaaacaaa cagaaaatta     1080

tttggtaaat aa                                                         1092


<210>  2
<211>  363
<212>  PRT
<213>  Mycoplasma bovis

<400>  2

Met Asn Tyr Lys Tyr Val Lys Pro Gly His Ala Thr Ser Asp Pro Asn 
1               5                   10                  15      


Glu Thr Val Arg Phe Leu Asp Val Asp Gly Lys Leu Ile Gln Glu Tyr 
            20                  25                  30          


Lys Pro Ser Glu Glu Thr Lys Lys Lys Leu Val Glu Met Tyr Lys Asn 
        35                  40                  45              


Met Ile Arg Ser Arg Gln Trp Asp Leu Tyr Ser Leu Thr Leu Gln Lys 
    50                  55                  60                  


Thr Gly Arg Leu Gly Thr Phe Ala Pro Ala Leu Gly Glu Glu Ala Ala 
65                  70                  75                  80  


Leu Thr Gly Ile Gly Tyr Asn Leu Asn Lys Glu Asp Trp Phe Ile Pro 
                85                  90                  95      


His Tyr Arg Val Leu Pro Thr Gln Leu Ala Arg Gly Ile Ser Met Asp 
            100                 105                 110         


Lys Ile Tyr Ser Tyr Trp Gln Gly Ser Glu Ile Gly Ser Ala Phe Ala 
        115                 120                 125             


Gly Thr Asn Thr Leu Pro Leu His Val Val Ile Gly Ser Gln Val Pro 
    130                 135                 140                 


Ile Ala Ala Gly Val Ala Gln Ala Leu Lys Phe Gln Gly Lys Lys Ala 
145                 150                 155                 160 


Leu Ala Met Val Thr Ile Gly Asn Gly Gly Thr Asn Glu Gly Glu Phe 
                165                 170                 175     


His Glu Gly Leu Asn Met Ala Ser Val Arg Lys Trp Pro Leu Val Thr 
            180                 185                 190         


Val Val Met Asn Asn Gln Trp Ala Ile Ser Val Pro Glu His Asn Ser 
        195                 200                 205             


Tyr Ile Val Lys Thr Leu Ser Gln Arg Ala Lys Ser Tyr Asp Met Pro 
    210                 215                 220                 


Gly Val Arg Val Asp Gly Asn Asp Leu Ile Ala Val Asn Glu Val Met 
225                 230                 235                 240 


Gln Glu Val Tyr Asp Phe Val Arg Glu Gly Asn Gly Pro Val Leu Val 
                245                 250                 255     


Glu Met Val Thr Trp Arg Gln Gly Gln His Thr Thr Ser Asp Asn Pro 
            260                 265                 270         


Arg Val Tyr Arg Ser Arg Glu Leu Glu Val Glu Lys Glu Lys Trp Glu 
        275                 280                 285             


Pro Phe His Arg Ile Glu Ala Tyr Leu Leu Ser Glu Lys Leu Ile Thr 
    290                 295                 300                 


Glu Glu Asp Ile Lys Val Trp Ser Glu Ala Ala Tyr Glu Glu Ala Lys 
305                 310                 315                 320 


Ala Ala Tyr Ala Leu Ser Lys Glu Leu Cys Glu Ala Thr Thr Phe Asp 
                325                 330                 335     


His Ile Tyr Asp Tyr Thr Tyr Glu Lys Leu Pro Ala Asp Leu Val Arg 
            340                 345                 350         


Gln Lys Glu Thr Asn Arg Lys Leu Phe Gly Lys 
        355                 360             


<210>  3
<211>  1371
<212>  DNA
<213>  Mycoplasma bovis

<400>  3
atgaaattat ttgaatcaat atcatcaaaa agagataata atatgttgct taaggcaaca       60

tttgctgatg aaactaaatg caatttttta attaaaaaag acaacagcat aactgaatat      120

catgaaaaga atgaagcatt agtatatttt agtaagaaag aatcattatc attttctgac      180

ttagaaggct tttttaaagg cttagcagta aatgccaaca gaaattatca agttgattta      240

gcttcgtttg caactgaaaa agttgaaata gttaaagtta ttgatgcatt tgttagagca      300

gtttattttg caaagggcga aatattttca gctagaaaaa aagatgaaaa ggaagaaatt      360

gaattagttc catttattga aactatttct gaacaagcta acgcacaatt taataaatcg      420

cttatcttag ccaaagcaac aaattttgct cgtgatttac aaattatgcc cccaaatatt      480

tgcaactctg aatttttagc tcaaaaagtt gctgaagatt tagaacaata caaaaacttg      540

aaagttactg ttttaaagaa aaaagaaatc gaagagttga agatgggtct tttactttca      600

gtaaacaaag gaagtgttta tgaacctaga gttgttgtta ttgaatacaa tggggacaaa      660

gattcaagtg aaaagactgt aatgattggt aaaggtatta cttttgattc aggtggatac      720

tcattaaaac cttctagatc aatggtttca atgaaatttg atatgtctgg ttcagctatt      780

gttgctgcta caatgaaagc tattgcacaa ttaaaaccaa agaaaaatgt ttctgcaata      840

atgtgcatta ctgataacag agttaacggt gatgcttcac ttcctgattc agtatgggta      900

gctatgaatg gcaaaagtgt tgaaattaat aatactgatg ctgaaggaag attggttatg      960

gctgatggct tagtttacgg agcaaaagtg ttgaatgcca ctagattaat tgacgttgca     1020

actttaactg gtgctatggt tgttgcactt ggacagacat acacaggcac atgggcaact     1080

agtgataaag cttgagaaga cataaagaaa gctgctgaaa atgctaacga attagtttga     1140

agaatgccgc ttgataaagc atttgcaaaa aacataaaat cttcaaaagt agccgattta     1200

aagaatactg acttttcagg aaatgcaggc tcatgttcag cagcaatgtt tttagaagaa     1260

tttacagaag gtgttgaaca tattcatctt gatgtagctg gaactgctga aatcagtgaa     1320

gtgccacaag gaattatggt taaaacttta actgaattaa gtttacttta a              1371


<210>  4
<211>  456
<212>  PRT
<213>  Mycoplasma bovis

<400>  4

Met Lys Leu Phe Glu Ser Ile Ser Ser Lys Arg Asp Asn Asn Met Leu 
1               5                   10                  15      


Leu Lys Ala Thr Phe Ala Asp Glu Thr Lys Cys Asn Phe Leu Ile Lys 
            20                  25                  30          


Lys Asp Asn Ser Ile Thr Glu Tyr His Glu Lys Asn Glu Ala Leu Val 
        35                  40                  45              


Tyr Phe Ser Lys Lys Glu Ser Leu Ser Phe Ser Asp Leu Glu Gly Phe 
    50                  55                  60                  


Phe Lys Gly Leu Ala Val Asn Ala Asn Arg Asn Tyr Gln Val Asp Leu 
65                  70                  75                  80  


Ala Ser Phe Ala Thr Glu Lys Val Glu Ile Val Lys Val Ile Asp Ala 
                85                  90                  95      


Phe Val Arg Ala Val Tyr Phe Ala Lys Gly Glu Ile Phe Ser Ala Arg 
            100                 105                 110         


Lys Lys Asp Glu Lys Glu Glu Ile Glu Leu Val Pro Phe Ile Glu Thr 
        115                 120                 125             


Ile Ser Glu Gln Ala Asn Ala Gln Phe Asn Lys Ser Leu Ile Leu Ala 
    130                 135                 140                 


Lys Ala Thr Asn Phe Ala Arg Asp Leu Gln Ile Met Pro Pro Asn Ile 
145                 150                 155                 160 


Cys Asn Ser Glu Phe Leu Ala Gln Lys Val Ala Glu Asp Leu Glu Gln 
                165                 170                 175     


Tyr Lys Asn Leu Lys Val Thr Val Leu Lys Lys Lys Glu Ile Glu Glu 
            180                 185                 190         


Leu Lys Met Gly Leu Leu Leu Ser Val Asn Lys Gly Ser Val Tyr Glu 
        195                 200                 205             


Pro Arg Val Val Val Ile Glu Tyr Asn Gly Asp Lys Asp Ser Ser Glu 
    210                 215                 220                 


Lys Thr Val Met Ile Gly Lys Gly Ile Thr Phe Asp Ser Gly Gly Tyr 
225                 230                 235                 240 


Ser Leu Lys Pro Ser Arg Ser Met Val Ser Met Lys Phe Asp Met Ser 
                245                 250                 255     


Gly Ser Ala Ile Val Ala Ala Thr Met Lys Ala Ile Ala Gln Leu Lys 
            260                 265                 270         


Pro Lys Lys Asn Val Ser Ala Ile Met Cys Ile Thr Asp Asn Arg Val 
        275                 280                 285             


Asn Gly Asp Ala Ser Leu Pro Asp Ser Val Trp Val Ala Met Asn Gly 
    290                 295                 300                 


Lys Ser Val Glu Ile Asn Asn Thr Asp Ala Glu Gly Arg Leu Val Met 
305                 310                 315                 320 


Ala Asp Gly Leu Val Tyr Gly Ala Lys Val Leu Asn Ala Thr Arg Leu 
                325                 330                 335     


Ile Asp Val Ala Thr Leu Thr Gly Ala Met Val Val Ala Leu Gly Gln 
            340                 345                 350         


Thr Tyr Thr Gly Thr Trp Ala Thr Ser Asp Lys Ala Trp Glu Asp Ile 
        355                 360                 365             


Lys Lys Ala Ala Glu Asn Ala Asn Glu Leu Val Trp Arg Met Pro Leu 
    370                 375                 380                 


Asp Lys Ala Phe Ala Lys Asn Ile Lys Ser Ser Lys Val Ala Asp Leu 
385                 390                 395                 400 


Lys Asn Thr Asp Phe Ser Gly Asn Ala Gly Ser Cys Ser Ala Ala Met 
                405                 410                 415     


Phe Leu Glu Glu Phe Thr Glu Gly Val Glu His Ile His Leu Asp Val 
            420                 425                 430         


Ala Gly Thr Ala Glu Ile Ser Glu Val Pro Gln Gly Ile Met Val Lys 
        435                 440                 445             


Thr Leu Thr Glu Leu Ser Leu Leu 
    450                 455     


<210>  5
<211>  1191
<212>  DNA
<213>  Mycoplasma bovis

<400>  5
atggcaaaac tagattttga ccgtagcaaa gaacacgtta atattggaac cattggccac       60

gttgaccatg gtaaaacaac tttaactgct gctattgcga cagtgttagc taaaaaaggt      120

ttatcagaag ctaaatcata cgatgcaatt gacaatgcac ctgaagaaaa agctcgtggt      180

ataacaatta atacatcaca catcgaatat aatactgaaa aacgtcacta tgcacacgtt      240

gactgtccag gtcacgctga ctacattaaa aacatgatta ctggtgctgc tcaaatggat      300

ggttcaattc ttgttgttgc tgcaactgac ggtgccatgc ctcaaacaaa agaacacgtt      360

cttcttgcaa aacaagttgg tgttccaaag atggttgttt tcttaaacaa atgtgacatg      420

attaagccag aagatgcaga aatgatcgac cttgttgaaa tggaagttcg tgaattactt      480

acaaaatatg gctttgacgg tgacaataca ccatttgttc gtggttcagc tctacaagct      540

ttacaaggca aaccagaata cgaagaaaac attctagaat taatgaatgc agttgacaca      600

tggatcgaaa ctcctgttaa ggactttgaa aaaccattct taatggctgt tgaagacgtt      660

ttcacaattt caggtcgtgg tacagttgct acaggtcgtg ttgaacgtgg tagattaagc      720

ttaaacgaag aagttgaaat tgtaggtctt aaaccaacaa agaaaacagt tgttacaggt      780

attgaaatgt tcagaaagaa cttaaaagaa gctcaagcag gtgacaacgc aggtctatta      840

cttcgtggtg ttgaaagatc agctattgaa cgtggtcaag ttcttgctaa accaggttct      900

attgttcctc atgctgaatt cgaagcagct atttatgcat taacaaaaga agaaggtggt      960

cgtcacactc cattctttgt taactataaa ccacaattct acttccgtac aacagacgtt     1020

acaggtggat tagaatttga aaaaggccgt gaattcgttc aacctggtga aaacgttaac     1080

ttaaaagtta aacttattgc acctatcgct gttgaagaag gaacaaagtt ctcgatccgt     1140

gaaggtggta gaacagttgg atatggttct gtaacaaaaa ttcttaaata a              1191


<210>  6
<211>  396
<212>  PRT
<213>  Mycoplasma bovis

<400>  6

Met Ala Lys Leu Asp Phe Asp Arg Ser Lys Glu His Val Asn Ile Gly 
1               5                   10                  15      


Thr Ile Gly His Val Asp His Gly Lys Thr Thr Leu Thr Ala Ala Ile 
            20                  25                  30          


Ala Thr Val Leu Ala Lys Lys Gly Leu Ser Glu Ala Lys Ser Tyr Asp 
        35                  40                  45              


Ala Ile Asp Asn Ala Pro Glu Glu Lys Ala Arg Gly Ile Thr Ile Asn 
    50                  55                  60                  


Thr Ser His Ile Glu Tyr Asn Thr Glu Lys Arg His Tyr Ala His Val 
65                  70                  75                  80  


Asp Cys Pro Gly His Ala Asp Tyr Ile Lys Asn Met Ile Thr Gly Ala 
                85                  90                  95      


Ala Gln Met Asp Gly Ser Ile Leu Val Val Ala Ala Thr Asp Gly Ala 
            100                 105                 110         


Met Pro Gln Thr Lys Glu His Val Leu Leu Ala Lys Gln Val Gly Val 
        115                 120                 125             


Pro Lys Met Val Val Phe Leu Asn Lys Cys Asp Met Ile Lys Pro Glu 
    130                 135                 140                 


Asp Ala Glu Met Ile Asp Leu Val Glu Met Glu Val Arg Glu Leu Leu 
145                 150                 155                 160 


Thr Lys Tyr Gly Phe Asp Gly Asp Asn Thr Pro Phe Val Arg Gly Ser 
                165                 170                 175     


Ala Leu Gln Ala Leu Gln Gly Lys Pro Glu Tyr Glu Glu Asn Ile Leu 
            180                 185                 190         


Glu Leu Met Asn Ala Val Asp Thr Trp Ile Glu Thr Pro Val Lys Asp 
        195                 200                 205             


Phe Glu Lys Pro Phe Leu Met Ala Val Glu Asp Val Phe Thr Ile Ser 
    210                 215                 220                 


Gly Arg Gly Thr Val Ala Thr Gly Arg Val Glu Arg Gly Arg Leu Ser 
225                 230                 235                 240 


Leu Asn Glu Glu Val Glu Ile Val Gly Leu Lys Pro Thr Lys Lys Thr 
                245                 250                 255     


Val Val Thr Gly Ile Glu Met Phe Arg Lys Asn Leu Lys Glu Ala Gln 
            260                 265                 270         


Ala Gly Asp Asn Ala Gly Leu Leu Leu Arg Gly Val Glu Arg Ser Ala 
        275                 280                 285             


Ile Glu Arg Gly Gln Val Leu Ala Lys Pro Gly Ser Ile Val Pro His 
    290                 295                 300                 


Ala Glu Phe Glu Ala Ala Ile Tyr Ala Leu Thr Lys Glu Glu Gly Gly 
305                 310                 315                 320 


Arg His Thr Pro Phe Phe Val Asn Tyr Lys Pro Gln Phe Tyr Phe Arg 
                325                 330                 335     


Thr Thr Asp Val Thr Gly Gly Leu Glu Phe Glu Lys Gly Arg Glu Phe 
            340                 345                 350         


Val Gln Pro Gly Glu Asn Val Asn Leu Lys Val Lys Leu Ile Ala Pro 
        355                 360                 365             


Ile Ala Val Glu Glu Gly Thr Lys Phe Ser Ile Arg Glu Gly Gly Arg 
    370                 375                 380                 


Thr Val Gly Tyr Gly Ser Val Thr Lys Ile Leu Lys 
385                 390                 395     


<210>  7
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding P48 truncate

<400>  7
gcttcatgtg gtgataaata ctttaaagaa actgaagttg atggagtgaa aactgttaca       60

acactttcac acatcgtgtc tcgtaaagga ttaaaactaa gagatggttt aaccgttgat      120

aatgcaccaa gggcagcatt tataactgat gaaggatctg tgcatgatga atcattcaat      180

caatctggtt gagaagcagt tcataaaatt tcatatgaac ttggattaga taaggcgcaa      240

gttagtggta ataaaaactt aagaaataaa gtttacgaac ctaaaaaggg tgaattagca      300

tcttcataca aaaacgctat tgatagtagt tttagataca ttgttctttg tggatttact      360

cacaaagcag cgctttatgg tttagaacca gaatacatta aaaagattaa agataataat      420

attgtcttta ttactgttga ctttgatatc caacaagatg caagcactgg cgaacctgca      480

gcaaaagcat ttgttgacaa aattggtcaa ggacgcttaa ttcctgtaat atttgacact      540

aaacaagctg catatattgc tggtcgtgct cttgctgact acttctcaaa aatttacaag      600

gataatcctg aaaaacgtac aataggtgcc ttcggtggta ttccttgacc atcagtttct      660

gactttatag ctggtacatt ccaaggtatt attgactgga ataaagaaca cccagaagct      720

aagactaaat cattaaataa cacaattgaa ttaaaaacat catttacttc tggtgaaccc      780

gttgcagttg ctgctattaa ctcagtaatt aaggccacag cttcatatcc agttgcagga      840

tcattatctt ctgacacagc taaagaaatt aagaagttag gcgataaaaa caaattcatt      900

attggtgttg acgctgacca aaagaatgca ttaaagggtc atagaatatt tacatcagtt      960

atgaaattaa ttggtcaagc tgtttataac gttttagctg acttatactc tcaaggtgaa     1020

aatagtctat cgcttcaacc aggctttgaa attggtaaaa aaaatggtga ggctaaagta     1080

tttggttatg gtgaaaatga agcaagcaaa tatgtaggtg ttgcaacttc aggattatta     1140

gactcaaaga atgatgagat tgctaataaa gcattggaag aagctactaa atactacgaa     1200

agtaaaaaag cggaaattca aaaaacttta tcaggtcaat tggaagaagc taaaaaagct     1260

ttaggtacaa aatgaccaga ccaacctgct gaccaatttg ggaaaatgat taactgattg     1320

gctaaagaaa cacaaaaata g                                               1341


<210>  8
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P48 truncate

<400>  8

Ala Ser Cys Gly Asp Lys Tyr Phe Lys Glu Thr Glu Val Asp Gly Val 
1               5                   10                  15      


Lys Thr Val Thr Thr Leu Ser His Ile Val Ser Arg Lys Gly Leu Lys 
            20                  25                  30          


Leu Arg Asp Gly Leu Thr Val Asp Asn Ala Pro Arg Ala Ala Phe Ile 
        35                  40                  45              


Thr Asp Glu Gly Ser Val His Asp Glu Ser Phe Asn Gln Ser Gly Trp 
    50                  55                  60                  


Glu Ala Val His Lys Ile Ser Tyr Glu Leu Gly Leu Asp Lys Ala Gln 
65                  70                  75                  80  


Val Ser Gly Asn Lys Asn Leu Arg Asn Lys Val Tyr Glu Pro Lys Lys 
                85                  90                  95      


Gly Glu Leu Ala Ser Ser Tyr Lys Asn Ala Ile Asp Ser Ser Phe Arg 
            100                 105                 110         


Tyr Ile Val Leu Cys Gly Phe Thr His Lys Ala Ala Leu Tyr Gly Leu 
        115                 120                 125             


Glu Pro Glu Tyr Ile Lys Lys Ile Lys Asp Asn Asn Ile Val Phe Ile 
    130                 135                 140                 


Thr Val Asp Phe Asp Ile Gln Gln Asp Ala Ser Thr Gly Glu Pro Ala 
145                 150                 155                 160 


Ala Lys Ala Phe Val Asp Lys Ile Gly Gln Gly Arg Leu Ile Pro Val 
                165                 170                 175     


Ile Phe Asp Thr Lys Gln Ala Ala Tyr Ile Ala Gly Arg Ala Leu Ala 
            180                 185                 190         


Asp Tyr Phe Ser Lys Ile Tyr Lys Asp Asn Pro Glu Lys Arg Thr Ile 
        195                 200                 205             


Gly Ala Phe Gly Gly Ile Pro Trp Pro Ser Val Ser Asp Phe Ile Ala 
    210                 215                 220                 


Gly Thr Phe Gln Gly Ile Ile Asp Trp Asn Lys Glu His Pro Glu Ala 
225                 230                 235                 240 


Lys Thr Lys Ser Leu Asn Asn Thr Ile Glu Leu Lys Thr Ser Phe Thr 
                245                 250                 255     


Ser Gly Glu Pro Val Ala Val Ala Ala Ile Asn Ser Val Ile Lys Ala 
            260                 265                 270         


Thr Ala Ser Tyr Pro Val Ala Gly Ser Leu Ser Ser Asp Thr Ala Lys 
        275                 280                 285             


Glu Ile Lys Lys Leu Gly Asp Lys Asn Lys Phe Ile Ile Gly Val Asp 
    290                 295                 300                 


Ala Asp Gln Lys Asn Ala Leu Lys Gly His Arg Ile Phe Thr Ser Val 
305                 310                 315                 320 


Met Lys Leu Ile Gly Gln Ala Val Tyr Asn Val Leu Ala Asp Leu Tyr 
                325                 330                 335     


Ser Gln Gly Glu Asn Ser Leu Ser Leu Gln Pro Gly Phe Glu Ile Gly 
            340                 345                 350         


Lys Lys Asn Gly Glu Ala Lys Val Phe Gly Tyr Gly Glu Asn Glu Ala 
        355                 360                 365             


Ser Lys Tyr Val Gly Val Ala Thr Ser Gly Leu Leu Asp Ser Lys Asn 
    370                 375                 380                 


Asp Glu Ile Ala Asn Lys Ala Leu Glu Glu Ala Thr Lys Tyr Tyr Glu 
385                 390                 395                 400 


Ser Lys Lys Ala Glu Ile Gln Lys Thr Leu Ser Gly Gln Leu Glu Glu 
                405                 410                 415     


Ala Lys Lys Ala Leu Gly Thr Lys 
            420                 


<210>  9
<211>  2124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding P81 truncate

<400>  9
gcagtgtctg ctaagtgtgg tggaactgta aattatgaag atctaggaaa agatgcaaaa       60

aaaatatcac ttggtgttag cttttcatct ggtcagccac aatgaaatac tatggcttca      120

ttaataaaat attataatga ggctcacaaa aatgataaac actttttacc tgtagaacta      180

aaacacttag gttcaggtta ccctgaaggt gaaaacacag tcatcaccga attaaaagca      240

aaaagaaatg aagttgttaa tttagctttt aactatggtt cactggcttc tagattagct      300

tcatctgaaa tgcgtgactt atacaaaatg gataaagttc taaactttga agataatgat      360

aaagatataa gtgttgattt aaagaacatt aacgagcaat ttgcaagagc aaattcaaac      420

accgaaaatc tacctaataa tggtacattt atgattccaa tgttaaagtc aatccaagtt      480

atgtctgcta acgcgccagt attacaatac atctttaaaa ctttcaagga taaaggtgct      540

aaatttgatg aatcgtttga aaactcaaca aaatacaaag aaattattga aaatggtaag      600

ggcgacgagg acgaagtaaa aaaattgtga ggtgaatttg aaagttcaca agaaagcgcc      660

gttaaaagtt taacaataag tagtagtaca ttcgaaaatc ttgaggaact tttaactttt      720

gctaacatag cgcaaaaaag ttttaaaaac tcagcagcca agaactctag attacatatc      780

ttaggtgttg atgacgtttc gggattaatt caatcactgc catatgcaat gattaatgca      840

gatgctaatg actttttcat ccagactggt cttgttaaga ataaaacaac agtaaattac      900

aaaaaaatta aagacaaaaa caacaagtca gttaaagcac tttcagaaat ttacaacaaa      960

ttcaaagact ctctaattgc caaatcgctt acattattag ctggtggtga atatacatct     1020

tcataccaaa caaaacacga atatgctttt ggtattggat caactgctgg atatagacat     1080

aactatattc cagacaaagc taaaaaagtt gttttcacac ttaaagatac tgctgttagt     1140

ggcgaaaaag atacagaatt taaaaatgta attaaaaaga caaaaaaagg cattgaccaa     1200

ttgcttgtta tttcaaaagg ccatgctaat aaagtttata aatcaacagt cgatatagat     1260

aaacttgatg ataaggaaaa aggctcatta aaatacagtt ataaatctct tgactctgca     1320

acagacagta aaatgtatga gatactcaag aaaataacga atactgatgc aaaagcaaaa     1380

aataataacc aatgactatt attcttaaga gaagataatg aaagcgatat taaagcagct     1440

aaaggaaaag gcgctgaaga agttggaacc gttattgaaa ctaagataag tggagcatct     1500

aagtataaaa ttttcttctt aaatgatgaa agtttattaa atagaaaaga actttcatca     1560

aaaggaacac ttcaagaaaa tgaattaata gtatttgctg tgcctggtaa atgaaataaa     1620

cataatgaaa agagagttat ttattcacaa ggtccttcac ttattggtat aagtagaggt     1680

gcaaaacctg atagagccgc taaaaatttt gctaagttcc ttacttctct ggacaaaata     1740

gatattacac ttagcaagta tgacaaagat atgaaaaaga ccgaagatag tgaagacaaa     1800

ccctacaaac aagttactcc tgcacaattt attagtgatg ctgcttcata tgtattccct     1860

gtaaaaggtt ttgaaaatac tgacacatct aagatcacaa ataagtacat tgttcataca     1920

tataacgaat taaaagaagc tgtaacaaat aaaaatgttg ttatatatga agaaccagct     1980

ggattccact catcatcatt tagagaaagc ttaggttcag catttaaatc agcatatcta     2040

aaagctaaaa atgatcaacc actagaagat ttcgataaag aaattattgg atcaattatt     2100

gcatcaagca gtcaaattct taaa                                            2124


<210>  10
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P81 truncate

<400>  10

Ala Val Ser Ala Lys Cys Gly Gly Thr Val Asn Tyr Glu Asp Leu Gly 
1               5                   10                  15      


Lys Asp Ala Lys Lys Ile Ser Leu Gly Val Ser Phe Ser Ser Gly Gln 
            20                  25                  30          


Pro Gln Trp Asn Thr Met Ala Ser Leu Ile Lys Tyr Tyr Asn Glu Ala 
        35                  40                  45              


His Lys Asn Asp Lys His Phe Leu Pro Val Glu Leu Lys His Leu Gly 
    50                  55                  60                  


Ser Gly Tyr Pro Glu Gly Glu Asn Thr Val Ile Thr Glu Leu Lys Ala 
65                  70                  75                  80  


Lys Arg Asn Glu Val Val Asn Leu Ala Phe Asn Tyr Gly Ser Leu Ala 
                85                  90                  95      


Ser Arg Leu Ala Ser Ser Glu Met Arg Asp Leu Tyr Lys Met Asp Lys 
            100                 105                 110         


Val Leu Asn Phe Glu Asp Asn Asp Lys Asp Ile Ser Val Asp Leu Lys 
        115                 120                 125             


Asn Ile Asn Glu Gln Phe Ala Arg Ala Asn Ser Asn Thr Glu Asn Leu 
    130                 135                 140                 


Pro Asn Asn Gly Thr Phe Met Ile Pro Met Leu Lys Ser Ile Gln Val 
145                 150                 155                 160 


Met Ser Ala Asn Ala Pro Val Leu Gln Tyr Ile Phe Lys Thr Phe Lys 
                165                 170                 175     


Asp Lys Gly Ala Lys Phe Asp Glu Ser Phe Glu Asn Ser Thr Lys Tyr 
            180                 185                 190         


Lys Glu Ile Ile Glu Asn Gly Lys Gly Asp Glu Asp Glu Val Lys Lys 
        195                 200                 205             


Leu Trp Gly Glu Phe Glu Ser Ser Gln Glu Ser Ala Val Lys Ser Leu 
    210                 215                 220                 


Thr Ile Ser Ser Ser Thr Phe Glu Asn Leu Glu Glu Leu Leu Thr Phe 
225                 230                 235                 240 


Ala Asn Ile Ala Gln Lys Ser Phe Lys Asn Ser Ala Ala Lys Asn Ser 
                245                 250                 255     


Arg Leu His Ile Leu Gly Val Asp Asp Val Ser Gly Leu Ile Gln Ser 
            260                 265                 270         


Leu Pro Tyr Ala Met Ile Asn Ala Asp Ala Asn Asp Phe Phe Ile Gln 
        275                 280                 285             


Thr Gly Leu Val Lys Asn Lys Thr Thr Val Asn Tyr Lys Lys Ile Lys 
    290                 295                 300                 


Asp Lys Asn Asn Lys Ser Val Lys Ala Leu Ser Glu Ile Tyr Asn Lys 
305                 310                 315                 320 


Phe Lys Asp Ser Leu Ile Ala Lys Ser Leu Thr Leu Leu Ala Gly Gly 
                325                 330                 335     


Glu Tyr Thr Ser Ser Tyr Gln Thr Lys His Glu Tyr Ala Phe Gly Ile 
            340                 345                 350         


Gly Ser Thr Ala Gly Tyr Arg His Asn Tyr Ile Pro Asp Lys Ala Lys 
        355                 360                 365             


Lys Val Val Phe Thr Leu Lys Asp Thr Ala Val Ser Gly Glu Lys Asp 
    370                 375                 380                 


Thr Glu Phe Lys Asn Val Ile Lys Lys Thr Lys Lys Gly Ile Asp Gln 
385                 390                 395                 400 


Leu Leu Val Ile Ser Lys Gly His Ala Asn Lys Val Tyr Lys Ser Thr 
                405                 410                 415     


Val Asp Ile Asp Lys Leu Asp Asp Lys Glu Lys Gly Ser Leu Lys Tyr 
            420                 425                 430         


Ser Tyr Lys Ser Leu Asp Ser Ala Thr Asp Ser Lys Met Tyr Glu Ile 
        435                 440                 445             


Leu Lys Lys Ile Thr Asn Thr Asp Ala Lys Ala Lys Asn Asn Asn Gln 
    450                 455                 460                 


Trp Leu Leu Phe Leu Arg Glu Asp Asn Glu Ser Asp Ile Lys Ala Ala 
465                 470                 475                 480 


Lys Gly Lys Gly Ala Glu Glu Val Gly Thr Val Ile Glu Thr Lys Ile 
                485                 490                 495     


Ser Gly Ala Ser Lys Tyr Lys Ile Phe Phe Leu Asn Asp Glu Ser Leu 
            500                 505                 510         


Leu Asn Arg Lys Glu Leu Ser Ser Lys Gly Thr Leu Gln Glu Asn Glu 
        515                 520                 525             


Leu Ile Val Phe Ala Val Pro Gly Lys Trp Asn Lys His Asn Glu Lys 
    530                 535                 540                 


Arg Val Ile Tyr Ser Gln Gly Pro Ser Leu Ile Gly Ile Ser Arg Gly 
545                 550                 555                 560 


Ala Lys Pro Asp Arg Ala Ala Lys Asn Phe Ala Lys Phe Leu Thr Ser 
                565                 570                 575     


Leu Asp Lys Ile Asp Ile Thr Leu Ser Lys Tyr Asp Lys Asp Met Lys 
            580                 585                 590         


Lys Thr Glu Asp Ser Glu Asp Lys Pro Tyr Lys Gln Val Thr Pro Ala 
        595                 600                 605             


Gln Phe Ile Ser Asp Ala Ala Ser Tyr Val Phe Pro Val Lys Gly Phe 
    610                 615                 620                 


Glu Asn Thr Asp Thr Ser Lys Ile Thr Asn Lys Tyr Ile Val His Thr 
625                 630                 635                 640 


Tyr Asn Glu Leu Lys Glu Ala Val Thr Asn Lys Asn Val Val Ile Tyr 
                645                 650                 655     


Glu Glu Pro Ala Gly Phe His Ser Ser Ser Phe Arg Glu Ser Leu Gly 
            660                 665                 670         


Ser Ala Phe Lys Ser Ala Tyr Leu Lys Ala Lys Asn Asp Gln Pro Leu 
        675                 680                 685             


Glu Asp Phe Asp Lys Glu Ile Ile Gly Ser Ile Ile Ala Ser Ser Ser 
    690                 695                 700                 


Gln Ile Leu Lys 
705             


<210>  11
<211>  2802
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding OppA truncate modified for expression in E. coli

<400>  11
atgaacgaag aataccaaga actggactac aaaaaatgga cgaatgtgct ggacggtcgc       60

ccggaatcgc tgtggaatct ggaactggaa tctaaaggct atgaatctgg tgaaagtaaa      120

gtgcagaacg atctgattgc acaaggcatc ctgcgtgcac cggctccggg taatcgtccg      180

gccgtgtccg aatactcatt tgatggcagc gtttcttatg gttcttggca gcgcagtgct      240

ctggaatccg cgcaaggcat tctgatccgt aaagaagcgc tgtttagccc gattgtgatc      300

aaaaccattc agggtcaatt cgtcaacgcc cgtccgtcgg tgtggcgcta taaactggaa      360

ctgggcagca aagttatcgt caccgacaac aatggtaaaa cgcatgaatt tgataacgac      420

ctggttaatg aattcccggc ggccgattcc gaaaccgtca accacaaagg caaatcaatc      480

gcaacgttca aaaacccgat ctatcaggcc acgagcaccg atgcaaaatc gattaacagc      540

aaacagtttc aagaagtgct gaagaaagcg aaaaaactgc agttcgaagt ggttaaaggc      600

caaaaatgga tcaacaataa aggtgaagct accaaatacg aagtcgtggc gaaagatttt      660

tattacagtt ggctgcgtac cacgggccgc aatgttgaac agcgtgaaaa actgctgagt      720

gaatccacgg attctcagta taaaaacggt cagaaaagcg acgaaatcga taaattcatc      780

aaccagaaat ggctgacccc gaactccaac tttttcacga aaagctctaa atactcgaac      840

gaatacgtgt atcagtttct gagcatcgac agttccaaat tctacaaaga agaactgttc      900

atcgaaggtg ataaactgac cttcaatccg ctgacggaag gcaaacaggg tagctttgac      960

ctgctgttcg aacatattgc gacctcacaa gatttttcgg cagctccgag ccagctgctg     1020

gaagaacacg atcaagaccc ggataaagtg ccggttaaac cgctgcgccc gcaggtggaa     1080

aaaaccacga ccgatgaata tcgtaaaatt ctgaacggca ccaaaggttc tctggccagt     1140

aaaatcggcc tgtactggta tggttttcat gaagatgacg ttctgaccgc tggccgttat     1200

tactatgcgg gttggaaccc gagcaatcgc gaagaaacgt acaaactgaa cccgcactat     1260

cgtaaagaaa atccgaaaga tccgattgcg aaatggaaag aatcccgtcg catcaaagaa     1320

taccgcacct ggtatcaggg cgactcactg aacgaaaata tttttaaaac ggccgtgaaa     1380

aacgattttc tgcgtggtaa actggcgttc gccccgcagt ctctgctgga caagaaagat     1440

ctggacctgt ttagcaatcg tcaacgcgat tacggcgcga gtttcattcg cgaaaacaat     1500

ccgacgacca gtccgtatca gttcctgacc tcttacatcc cgtatagtca aaaacatacc     1560

aacgaaacga aattcaactt caacgaacac ttcgcaaaac tggctttcgg tgcgtccctg     1620

aaagaaattc gcgaaggcgg taaaccgacc aatctgaaag ataaactggg cggtaccgca     1680

gtggcttttc gtacgctgat taactcagcc atcaattggg aatacctggc aaaatacatc     1740

tccaacgaca agaaaaccgc ctgggtttca ctgattgcac cgaacacggc catccaggca     1800

agcgatcaaa atggcaaaat cgtgcagccg gctgaatttg cggacaaatt caatgaacag     1860

tttttcgttg atgctcaggg taacaaagtt gcgacggtca ccccgaaaga aaataaagac     1920

aaatctaccg tccagagtga tgccgaacgc ttcaaaagcg caaaattcaa agaaatccag     1980

gcggaagtga agaaaattct ggacaaatac tacaaagata acaacctgaa cgcggacaaa     2040

gataaagtcg aatggaccct gattaaccgc aatgtgggct cgtttaatcc gccgctgctg     2100

gaacagctgg tccgttggat tccggacctg tacaaagctc tggacccgcg tctgagcgcg     2160

acctataaaa aatttgatgc ccgtgaagaa tgggtttctg cgattgcctc ccatacctca     2220

tacgccaact tcgcaagtat tcgttatgac acgaacaata tcggcgcggg ttatgatggc     2280

ctgggtctgt catcgctgcg cgtgattctg gttctgatca actctgatgc cgaactgcag     2340

aattccctgc gtaaatcatt tccgcaactg gtcaaagtgg cagacgaatt cgtgaaattc     2400

atgaacgatt ccaaaaacca gttcaaatgg agcgttgact tcaaacattg gaaagatgtc     2460

gaaagtaaat actgggatga cctgaacgat gacccgtcgg tttacaaatg gaacgaaagc     2520

gagaaaaaac tggaacgcaa cacggatacc tacacgaaat ggacccacct gtcggcggcc     2580

agcgcagaat ttttcgttaa atatgctaac tcgctgccgc tggaagataa tattgcactg     2640

tcgaacgaac tgagcaatta ctatggtcgc gtcccggaac cggcttttct gatcaacaaa     2700

gatcagttca tcatctcttt cctgtcaccg tcgctgagcc gtccgtacac cggcaccgac     2760

gcactgtggt tcgcagactt tgtcatccgt gataataaat aa                        2802


<210>  12
<211>  933
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OppA truncate

<400>  12

Met Asn Glu Glu Tyr Gln Glu Leu Asp Tyr Lys Lys Trp Thr Asn Val 
1               5                   10                  15      


Leu Asp Gly Arg Pro Glu Ser Leu Trp Asn Leu Glu Leu Glu Ser Lys 
            20                  25                  30          


Gly Tyr Glu Ser Gly Glu Ser Lys Val Gln Asn Asp Leu Ile Ala Gln 
        35                  40                  45              


Gly Ile Leu Arg Ala Pro Ala Pro Gly Asn Arg Pro Ala Val Ser Glu 
    50                  55                  60                  


Tyr Ser Phe Asp Gly Ser Val Ser Tyr Gly Ser Trp Gln Arg Ser Ala 
65                  70                  75                  80  


Leu Glu Ser Ala Gln Gly Ile Leu Ile Arg Lys Glu Ala Leu Phe Ser 
                85                  90                  95      


Pro Ile Val Ile Lys Thr Ile Gln Gly Gln Phe Val Asn Ala Arg Pro 
            100                 105                 110         


Ser Val Trp Arg Tyr Lys Leu Glu Leu Gly Ser Lys Val Ile Val Thr 
        115                 120                 125             


Asp Asn Asn Gly Lys Thr His Glu Phe Asp Asn Asp Leu Val Asn Glu 
    130                 135                 140                 


Phe Pro Ala Ala Asp Ser Glu Thr Val Asn His Lys Gly Lys Ser Ile 
145                 150                 155                 160 


Ala Thr Phe Lys Asn Pro Ile Tyr Gln Ala Thr Ser Thr Asp Ala Lys 
                165                 170                 175     


Ser Ile Asn Ser Lys Gln Phe Gln Glu Val Leu Lys Lys Ala Lys Lys 
            180                 185                 190         


Leu Gln Phe Glu Val Val Lys Gly Gln Lys Trp Ile Asn Asn Lys Gly 
        195                 200                 205             


Glu Ala Thr Lys Tyr Glu Val Val Ala Lys Asp Phe Tyr Tyr Ser Trp 
    210                 215                 220                 


Leu Arg Thr Thr Gly Arg Asn Val Glu Gln Arg Glu Lys Leu Leu Ser 
225                 230                 235                 240 


Glu Ser Thr Asp Ser Gln Tyr Lys Asn Gly Gln Lys Ser Asp Glu Ile 
                245                 250                 255     


Asp Lys Phe Ile Asn Gln Lys Trp Leu Thr Pro Asn Ser Asn Phe Phe 
            260                 265                 270         


Thr Lys Ser Ser Lys Tyr Ser Asn Glu Tyr Val Tyr Gln Phe Leu Ser 
        275                 280                 285             


Ile Asp Ser Ser Lys Phe Tyr Lys Glu Glu Leu Phe Ile Glu Gly Asp 
    290                 295                 300                 


Lys Leu Thr Phe Asn Pro Leu Thr Glu Gly Lys Gln Gly Ser Phe Asp 
305                 310                 315                 320 


Leu Leu Phe Glu His Ile Ala Thr Ser Gln Asp Phe Ser Ala Ala Pro 
                325                 330                 335     


Ser Gln Leu Leu Glu Glu His Asp Gln Asp Pro Asp Lys Val Pro Val 
            340                 345                 350         


Lys Pro Leu Arg Pro Gln Val Glu Lys Thr Thr Thr Asp Glu Tyr Arg 
        355                 360                 365             


Lys Ile Leu Asn Gly Thr Lys Gly Ser Leu Ala Ser Lys Ile Gly Leu 
    370                 375                 380                 


Tyr Trp Tyr Gly Phe His Glu Asp Asp Val Leu Thr Ala Gly Arg Tyr 
385                 390                 395                 400 


Tyr Tyr Ala Gly Trp Asn Pro Ser Asn Arg Glu Glu Thr Tyr Lys Leu 
                405                 410                 415     


Asn Pro His Tyr Arg Lys Glu Asn Pro Lys Asp Pro Ile Ala Lys Trp 
            420                 425                 430         


Lys Glu Ser Arg Arg Ile Lys Glu Tyr Arg Thr Trp Tyr Gln Gly Asp 
        435                 440                 445             


Ser Leu Asn Glu Asn Ile Phe Lys Thr Ala Val Lys Asn Asp Phe Leu 
    450                 455                 460                 


Arg Gly Lys Leu Ala Phe Ala Pro Gln Ser Leu Leu Asp Lys Lys Asp 
465                 470                 475                 480 


Leu Asp Leu Phe Ser Asn Arg Gln Arg Asp Tyr Gly Ala Ser Phe Ile 
                485                 490                 495     


Arg Glu Asn Asn Pro Thr Thr Ser Pro Tyr Gln Phe Leu Thr Ser Tyr 
            500                 505                 510         


Ile Pro Tyr Ser Gln Lys His Thr Asn Glu Thr Lys Phe Asn Phe Asn 
        515                 520                 525             


Glu His Phe Ala Lys Leu Ala Phe Gly Ala Ser Leu Lys Glu Ile Arg 
    530                 535                 540                 


Glu Gly Gly Lys Pro Thr Asn Leu Lys Asp Lys Leu Gly Gly Thr Ala 
545                 550                 555                 560 


Val Ala Phe Arg Thr Leu Ile Asn Ser Ala Ile Asn Trp Glu Tyr Leu 
                565                 570                 575     


Ala Lys Tyr Ile Ser Asn Asp Lys Lys Thr Ala Trp Val Ser Leu Ile 
            580                 585                 590         


Ala Pro Asn Thr Ala Ile Gln Ala Ser Asp Gln Asn Gly Lys Ile Val 
        595                 600                 605             


Gln Pro Ala Glu Phe Ala Asp Lys Phe Asn Glu Gln Phe Phe Val Asp 
    610                 615                 620                 


Ala Gln Gly Asn Lys Val Ala Thr Val Thr Pro Lys Glu Asn Lys Asp 
625                 630                 635                 640 


Lys Ser Thr Val Gln Ser Asp Ala Glu Arg Phe Lys Ser Ala Lys Phe 
                645                 650                 655     


Lys Glu Ile Gln Ala Glu Val Lys Lys Ile Leu Asp Lys Tyr Tyr Lys 
            660                 665                 670         


Asp Asn Asn Leu Asn Ala Asp Lys Asp Lys Val Glu Trp Thr Leu Ile 
        675                 680                 685             


Asn Arg Asn Val Gly Ser Phe Asn Pro Pro Leu Leu Glu Gln Leu Val 
    690                 695                 700                 


Arg Trp Ile Pro Asp Leu Tyr Lys Ala Leu Asp Pro Arg Leu Ser Ala 
705                 710                 715                 720 


Thr Tyr Lys Lys Phe Asp Ala Arg Glu Glu Trp Val Ser Ala Ile Ala 
                725                 730                 735     


Ser His Thr Ser Tyr Ala Asn Phe Ala Ser Ile Arg Tyr Asp Thr Asn 
            740                 745                 750         


Asn Ile Gly Ala Gly Tyr Asp Gly Leu Gly Leu Ser Ser Leu Arg Val 
        755                 760                 765             


Ile Leu Val Leu Ile Asn Ser Asp Ala Glu Leu Gln Asn Ser Leu Arg 
    770                 775                 780                 


Lys Ser Phe Pro Gln Leu Val Lys Val Ala Asp Glu Phe Val Lys Phe 
785                 790                 795                 800 


Met Asn Asp Ser Lys Asn Gln Phe Lys Trp Ser Val Asp Phe Lys His 
                805                 810                 815     


Trp Lys Asp Val Glu Ser Lys Tyr Trp Asp Asp Leu Asn Asp Asp Pro 
            820                 825                 830         


Ser Val Tyr Lys Trp Asn Glu Ser Glu Lys Lys Leu Glu Arg Asn Thr 
        835                 840                 845             


Asp Thr Tyr Thr Lys Trp Thr His Leu Ser Ala Ala Ser Ala Glu Phe 
    850                 855                 860                 


Phe Val Lys Tyr Ala Asn Ser Leu Pro Leu Glu Asp Asn Ile Ala Leu 
865                 870                 875                 880 


Ser Asn Glu Leu Ser Asn Tyr Tyr Gly Arg Val Pro Glu Pro Ala Phe 
                885                 890                 895     


Leu Ile Asn Lys Asp Gln Phe Ile Ile Ser Phe Leu Ser Pro Ser Leu 
            900                 905                 910         


Ser Arg Pro Tyr Thr Gly Thr Asp Ala Leu Trp Phe Ala Asp Phe Val 
        915                 920                 925             


Ile Arg Asp Asn Lys 
    930             


<210>  13
<211>  1785
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding LppB truncate modified for 
       expression in E. coli

<400>  13
gatggcaatg aaaccaaatc aatcgtgttc caaatcggtc atcgcaaatc ctggccgctg       60

tccggtggcc tggctccgtt cgcagaatat tacaacaaaa cctttaaagg ccagaaagat      120

ttcatgccgg tcaaaatgag ctatgcggat gaccataata ccacgtcgga atttgtgctg      180

gcgcgtcgcg ccaaagataa catcgaaagt ggtaacttca aagacgtgcc gaacattatc      240

ctgggcgcac agtccggtgc ttatgttatc aaccaataca atcgtctgat ggatctgagt      300

gacgtcggca ttaagaaaga tatcttcaac cagtccatcg cagacctgca cagcatcctg      360

tctggtcaag ctgataaaag ccgcgtgtat aacattccgt tcgacaatgc ggatacggac      420

gcactggctt ttaacctgga tgccctgtac ttcatgctga aaatgattga agacggcaaa      480

ggtgaagttg attggaccgc agacatcccg aaacgtgcta aagaagcggc ccagccgggc      540

aagggtaatg atctgccgga aaaatctatt tggcgcgcgc tgaaactgaa agaaggcgaa      600

ccgtttaaag gtttcaaagt tgatgaaaaa accttcaaaa gcatggaaaa aatccgtgaa      660

ctggcagtca aattccatga tggcaccacg ctggaccaga gcaaagtgga tgctgacacc      720

attacgggtg aagttctgtc tatcgattat aacgcgcaag cctttctgaa agaatttaaa      780

tctcgtatcg gcaaaacgga aaatgatgtt atctggaaac tggtcctgac gggtgaccag      840

aaaaaaccga cccgcgttga atataacctg ccgaacaatc cggaatacgt caaaatcttt      900

aaagaactgt gggataccta taccacgaaa tcaatcaaac gtcatgtgaa gaaagtgaac      960

gaaaattcga aagtgtttag ctctatcaaa tacatggaac acggcatcca ggaatggggt     1020

tcatggaaca ttcgcgaatt taaaagtgcg atttccatcc tggcctcggt cggcacgaat     1080

caacgtatgc tgagttccgt gagccgcaaa ctgttttcta aactgaaaga tggcaccctg     1140

gtgaaagact tcgagaaaaa caacgcaaaa ttcgaagatg tttacctgct gccgcagctg     1200

acgctgatga gtgaaggcaa agatcaaatt tttgcagaag gcggttcatc gatcctggct     1260

gtggatacca gcaaagaacg tgacaccaac acgccggaaa atcgcgccac gaaaaaattt     1320

ctgtcttggc tgtataccgg caaaaacacc cacacgggca ccgaagaaga aaattggaaa     1380

accttcgcca aactgagcgg ttacattatg ccgctgaaag atgttatctc tgacgcaaac     1440

gaaaaatgga tgaaagatga aatcgaacgt ctgctgaaag aagtgcgcga aatcgagaaa     1500

aaaccgaacg cgacggaaac cgaaaaagaa gttgccaaca aaaaattttt caccctgaac     1560

aatctgcgtt cagcgctggt ctcactgcag tcgattctga acctggagaa aaacaaagat     1620

aaagtgctgg ttaaagcgag tgttacggat gacgccacca gccaaattgc aggcgctatc     1680

gatggtgccc tgtatcgcag caccattcat gacggccacg aaatcgctga cgcagacaaa     1740

ctggtggaag gcttcaaaca aagtgtgcaa aatacgccga aataa                     1785


<210>  14
<211>  594
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LppB truncate

<400>  14

Asp Gly Asn Glu Thr Lys Ser Ile Val Phe Gln Ile Gly His Arg Lys 
1               5                   10                  15      


Ser Trp Pro Leu Ser Gly Gly Leu Ala Pro Phe Ala Glu Tyr Tyr Asn 
            20                  25                  30          


Lys Thr Phe Lys Gly Gln Lys Asp Phe Met Pro Val Lys Met Ser Tyr 
        35                  40                  45              


Ala Asp Asp His Asn Thr Thr Ser Glu Phe Val Leu Ala Arg Arg Ala 
    50                  55                  60                  


Lys Asp Asn Ile Glu Ser Gly Asn Phe Lys Asp Val Pro Asn Ile Ile 
65                  70                  75                  80  


Leu Gly Ala Gln Ser Gly Ala Tyr Val Ile Asn Gln Tyr Asn Arg Leu 
                85                  90                  95      


Met Asp Leu Ser Asp Val Gly Ile Lys Lys Asp Ile Phe Asn Gln Ser 
            100                 105                 110         


Ile Ala Asp Leu His Ser Ile Leu Ser Gly Gln Ala Asp Lys Ser Arg 
        115                 120                 125             


Val Tyr Asn Ile Pro Phe Asp Asn Ala Asp Thr Asp Ala Leu Ala Phe 
    130                 135                 140                 


Asn Leu Asp Ala Leu Tyr Phe Met Leu Lys Met Ile Glu Asp Gly Lys 
145                 150                 155                 160 


Gly Glu Val Asp Trp Thr Ala Asp Ile Pro Lys Arg Ala Lys Glu Ala 
                165                 170                 175     


Ala Gln Pro Gly Lys Gly Asn Asp Leu Pro Glu Lys Ser Ile Trp Arg 
            180                 185                 190         


Ala Leu Lys Leu Lys Glu Gly Glu Pro Phe Lys Gly Phe Lys Val Asp 
        195                 200                 205             


Glu Lys Thr Phe Lys Ser Met Glu Lys Ile Arg Glu Leu Ala Val Lys 
    210                 215                 220                 


Phe His Asp Gly Thr Thr Leu Asp Gln Ser Lys Val Asp Ala Asp Thr 
225                 230                 235                 240 


Ile Thr Gly Glu Val Leu Ser Ile Asp Tyr Asn Ala Gln Ala Phe Leu 
                245                 250                 255     


Lys Glu Phe Lys Ser Arg Ile Gly Lys Thr Glu Asn Asp Val Ile Trp 
            260                 265                 270         


Lys Leu Val Leu Thr Gly Asp Gln Lys Lys Pro Thr Arg Val Glu Tyr 
        275                 280                 285             


Asn Leu Pro Asn Asn Pro Glu Tyr Val Lys Ile Phe Lys Glu Leu Trp 
    290                 295                 300                 


Asp Thr Tyr Thr Thr Lys Ser Ile Lys Arg His Val Lys Lys Val Asn 
305                 310                 315                 320 


Glu Asn Ser Lys Val Phe Ser Ser Ile Lys Tyr Met Glu His Gly Ile 
                325                 330                 335     


Gln Glu Trp Gly Ser Trp Asn Ile Arg Glu Phe Lys Ser Ala Ile Ser 
            340                 345                 350         


Ile Leu Ala Ser Val Gly Thr Asn Gln Arg Met Leu Ser Ser Val Ser 
        355                 360                 365             


Arg Lys Leu Phe Ser Lys Leu Lys Asp Gly Thr Leu Val Lys Asp Phe 
    370                 375                 380                 


Glu Lys Asn Asn Ala Lys Phe Glu Asp Val Tyr Leu Leu Pro Gln Leu 
385                 390                 395                 400 


Thr Leu Met Ser Glu Gly Lys Asp Gln Ile Phe Ala Glu Gly Gly Ser 
                405                 410                 415     


Ser Ile Leu Ala Val Asp Thr Ser Lys Glu Arg Asp Thr Asn Thr Pro 
            420                 425                 430         


Glu Asn Arg Ala Thr Lys Lys Phe Leu Ser Trp Leu Tyr Thr Gly Lys 
        435                 440                 445             


Asn Thr His Thr Gly Thr Glu Glu Glu Asn Trp Lys Thr Phe Ala Lys 
    450                 455                 460                 


Leu Ser Gly Tyr Ile Met Pro Leu Lys Asp Val Ile Ser Asp Ala Asn 
465                 470                 475                 480 


Glu Lys Trp Met Lys Asp Glu Ile Glu Arg Leu Leu Lys Glu Val Arg 
                485                 490                 495     


Glu Ile Glu Lys Lys Pro Asn Ala Thr Glu Thr Glu Lys Glu Val Ala 
            500                 505                 510         


Asn Lys Lys Phe Phe Thr Leu Asn Asn Leu Arg Ser Ala Leu Val Ser 
        515                 520                 525             


Leu Gln Ser Ile Leu Asn Leu Glu Lys Asn Lys Asp Lys Val Leu Val 
    530                 535                 540                 


Lys Ala Ser Val Thr Asp Asp Ala Thr Ser Gln Ile Ala Gly Ala Ile 
545                 550                 555                 560 


Asp Gly Ala Leu Tyr Arg Ser Thr Ile His Asp Gly His Glu Ile Ala 
                565                 570                 575     


Asp Ala Asp Lys Leu Val Glu Gly Phe Lys Gln Ser Val Gln Asn Thr 
            580                 585                 590         


Pro Lys 
        


<210>  15
<211>  999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding PepQ truncate modified for 
       expression in E. coli

<400>  15
atggttagca gcgccccgca gacccgcctg tggtattcca acgtccaaac gaccgatggc       60

tacattatta ttgaaaaaga caaagcgtac ctgtttgttg attcacgtta catcgaatac      120

tgcgagaaaa acgcgaaaaa tgtggaagtt cgcctgctgg ccggtaaatc gctgaaagaa      180

tttttcgatc agaaaggcta caagaaagtg gcattcgaaa aagactacat cgtttacgat      240

gaattcgacc gtctggtcaa actgatcaac ccgaaaacca ttgcgtttat caaaggtcaa      300

gaactgcgca ttaaaaaatc agaagaagaa atccaggcaa tgcaagaagt tatcaacatc      360

agcctgaaag cttatgaaaa actgatctct tggatcatcc cgggcatgac cgaaaaacag      420

atcgcgacga aactgaacca cctgatgaaa ctgtacggtg cccaaaaaga atccttcgat      480

gaaattgtgg caagtggccc gaattccgct gaaccgcatc accatccgac ggatcgtcgc      540

attcgtgacg gtgaactgct gaaaatcgat tttggtgcgc tgtataaagg cttcagtgcc      600

gacattaccc gtacggttat cctgggccgc cagaacgtct ccgataaacc ggaacaagaa      660

aaaattctgg aaatcgtgaa agaagcggcc cgtctgggtc gcgaagcagt taaaccgggc      720

attaaagcta gcgatattga caaaatctgt cgtgactata tccagaacaa aggctatggt      780

tcatacttcg tccactcgac cggccatggt ctgggcattg atgtccacga actgccgaat      840

gtgagctctc attctgacta tatcctggaa gaaggtatga ttatcacggt ggaaccgggt      900

atttacatcc cgggtctggg cggtgcacgt attgaagatg atgtgctggt tacggcagac      960

ggccaccgcg tgctgacccg cccggaagaa gcgatgtga                             999


<210>  16
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PepQ truncate

<400>  16

Met Val Ser Ser Ala Pro Gln Thr Arg Leu Trp Tyr Ser Asn Val Gln 
1               5                   10                  15      


Thr Thr Asp Gly Tyr Ile Ile Ile Glu Lys Asp Lys Ala Tyr Leu Phe 
            20                  25                  30          


Val Asp Ser Arg Tyr Ile Glu Tyr Cys Glu Lys Asn Ala Lys Asn Val 
        35                  40                  45              


Glu Val Arg Leu Leu Ala Gly Lys Ser Leu Lys Glu Phe Phe Asp Gln 
    50                  55                  60                  


Lys Gly Tyr Lys Lys Val Ala Phe Glu Lys Asp Tyr Ile Val Tyr Asp 
65                  70                  75                  80  


Glu Phe Asp Arg Leu Val Lys Leu Ile Asn Pro Lys Thr Ile Ala Phe 
                85                  90                  95      


Ile Lys Gly Gln Glu Leu Arg Ile Lys Lys Ser Glu Glu Glu Ile Gln 
            100                 105                 110         


Ala Met Gln Glu Val Ile Asn Ile Ser Leu Lys Ala Tyr Glu Lys Leu 
        115                 120                 125             


Ile Ser Trp Ile Ile Pro Gly Met Thr Glu Lys Gln Ile Ala Thr Lys 
    130                 135                 140                 


Leu Asn His Leu Met Lys Leu Tyr Gly Ala Gln Lys Glu Ser Phe Asp 
145                 150                 155                 160 


Glu Ile Val Ala Ser Gly Pro Asn Ser Ala Glu Pro His His His Pro 
                165                 170                 175     


Thr Asp Arg Arg Ile Arg Asp Gly Glu Leu Leu Lys Ile Asp Phe Gly 
            180                 185                 190         


Ala Leu Tyr Lys Gly Phe Ser Ala Asp Ile Thr Arg Thr Val Ile Leu 
        195                 200                 205             


Gly Arg Gln Asn Val Ser Asp Lys Pro Glu Gln Glu Lys Ile Leu Glu 
    210                 215                 220                 


Ile Val Lys Glu Ala Ala Arg Leu Gly Arg Glu Ala Val Lys Pro Gly 
225                 230                 235                 240 


Ile Lys Ala Ser Asp Ile Asp Lys Ile Cys Arg Asp Tyr Ile Gln Asn 
                245                 250                 255     


Lys Gly Tyr Gly Ser Tyr Phe Val His Ser Thr Gly His Gly Leu Gly 
            260                 265                 270         


Ile Asp Val His Glu Leu Pro Asn Val Ser Ser His Ser Asp Tyr Ile 
        275                 280                 285             


Leu Glu Glu Gly Met Ile Ile Thr Val Glu Pro Gly Ile Tyr Ile Pro 
    290                 295                 300                 


Gly Leu Gly Gly Ala Arg Ile Glu Asp Asp Val Leu Val Thr Ala Asp 
305                 310                 315                 320 


Gly His Arg Val Leu Thr Arg Pro Glu Glu Ala Met 
                325                 330         


<210>  17
<211>  1086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding O256 modified for expression in E. 
       coli

<400>  17
atgagcaaat acgacgaact gaaaacgaaa ctggtccaat acatgagcat cgaagcaatg       60

agccgctacg aagaaccggt ggtgaaacaa ctgaaagaaa acatcaaatc agaaaacttc      120

gaattctcgc gtgatggttt tggcagcctg attatcacca aacgctcaaa agtcgcaaac      180

gctccgaaag tgatgatcgc ggcccacatg gacgaagtcg gttatctggt gcgtaatatt      240

gaagataacg gcaatctgct ggttagctct gtcggcggta tttggccgac cgcggtgatc      300

ggtacgaaag ccaaagtggt taccaacaaa gataacaaag aagcaatcgg tgttttcggc      360

cataccagca ttcacatcct ggaagcagaa aaacgcacga aagctccgct ggaaaaagaa      420

ctgtttgtgg attgcggctt cctgaacaaa caggaagcca ttgacttcgg tatcgaagtt      480

ggcgatcgtg tctatatgtc tggtgaaacc atcgaactgc gcaacgacct gctgggcggt      540

aaagcaatgg ataatcgtgc tggcgtcacg gtgattgaca tgctggcgaa ccgcattaaa      600

gatatcgacc tgccgaatga tctgtacctg gtgggtaccg ttcaagaaga agtgggttgc      660

cgtggcgcaa aaacgagtgt ttccctgatc aacccggatg tcgcgttcgc cattgacacc      720

ggcgcttccc atgataccac gggttgtatc aaaggcgtgc cgtcactggg taaaggcgtt      780

gcgctgctga ttcaggactc ggccgtcatg accgatccga aactggtgaa tatgctgacg      840

aaaattgccc aagaaaaaga catcccggtt tataaataca ttgcggaagg cggtggcacc      900

gatggtgccg ttctgcagta tggcattggt ggcgtcccga ccattacgct gtccatcccg      960

caacgctacc tgcactctcc gatcggtgtg gcaagtctgg ttgacattca ggctacgctg     1020

gatctgatta cggaatttgt gaaagtcttc gataacaata aaaacgctga actgcatacc     1080

aactga                                                                1086


<210>  18
<211>  361
<212>  PRT
<213>  Mycoplasma bovis

<400>  18

Met Ser Lys Tyr Asp Glu Leu Lys Thr Lys Leu Val Gln Tyr Met Ser 
1               5                   10                  15      


Ile Glu Ala Met Ser Arg Tyr Glu Glu Pro Val Val Lys Gln Leu Lys 
            20                  25                  30          


Glu Asn Ile Lys Ser Glu Asn Phe Glu Phe Ser Arg Asp Gly Phe Gly 
        35                  40                  45              


Ser Leu Ile Ile Thr Lys Arg Ser Lys Val Ala Asn Ala Pro Lys Val 
    50                  55                  60                  


Met Ile Ala Ala His Met Asp Glu Val Gly Tyr Leu Val Arg Asn Ile 
65                  70                  75                  80  


Glu Asp Asn Gly Asn Leu Leu Val Ser Ser Val Gly Gly Ile Trp Pro 
                85                  90                  95      


Thr Ala Val Ile Gly Thr Lys Ala Lys Val Val Thr Asn Lys Asp Asn 
            100                 105                 110         


Lys Glu Ala Ile Gly Val Phe Gly His Thr Ser Ile His Ile Leu Glu 
        115                 120                 125             


Ala Glu Lys Arg Thr Lys Ala Pro Leu Glu Lys Glu Leu Phe Val Asp 
    130                 135                 140                 


Cys Gly Phe Leu Asn Lys Gln Glu Ala Ile Asp Phe Gly Ile Glu Val 
145                 150                 155                 160 


Gly Asp Arg Val Tyr Met Ser Gly Glu Thr Ile Glu Leu Arg Asn Asp 
                165                 170                 175     


Leu Leu Gly Gly Lys Ala Met Asp Asn Arg Ala Gly Val Thr Val Ile 
            180                 185                 190         


Asp Met Leu Ala Asn Arg Ile Lys Asp Ile Asp Leu Pro Asn Asp Leu 
        195                 200                 205             


Tyr Leu Val Gly Thr Val Gln Glu Glu Val Gly Cys Arg Gly Ala Lys 
    210                 215                 220                 


Thr Ser Val Ser Leu Ile Asn Pro Asp Val Ala Phe Ala Ile Asp Thr 
225                 230                 235                 240 


Gly Ala Ser His Asp Thr Thr Gly Cys Ile Lys Gly Val Pro Ser Leu 
                245                 250                 255     


Gly Lys Gly Val Ala Leu Leu Ile Gln Asp Ser Ala Val Met Thr Asp 
            260                 265                 270         


Pro Lys Leu Val Asn Met Leu Thr Lys Ile Ala Gln Glu Lys Asp Ile 
        275                 280                 285             


Pro Val Tyr Lys Tyr Ile Ala Glu Gly Gly Gly Thr Asp Gly Ala Val 
    290                 295                 300                 


Leu Gln Tyr Gly Ile Gly Gly Val Pro Thr Ile Thr Leu Ser Ile Pro 
305                 310                 315                 320 


Gln Arg Tyr Leu His Ser Pro Ile Gly Val Ala Ser Leu Val Asp Ile 
                325                 330                 335     


Gln Ala Thr Leu Asp Leu Ile Thr Glu Phe Val Lys Val Phe Asp Asn 
            340                 345                 350         


Asn Lys Asn Ala Glu Leu His Thr Asn 
        355                 360     


<210>  19
<211>  1188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding DeoB modified for expression in E. 
       coli

<400>  19
atggctaaat tcaaacgcat cttcatgatc gtgacggacg gcctgggtat tggcccggac       60

aaagaccaga aaaaattcgg tgacgcaggt gcgaacacgc tgtatagcgc ctctctgagc      120

tctatgtttt tcatcgatac ctggaaaaaa ctgggcatcg gtaacgtggc ccagctggaa      180

ggcaattatc atgttaaatc accgctggcg tacgtgtcgc gtattcaaga agttagcaat      240

gcaaaagata cgctggctgg ccactgggaa atgatgggta tcaaaaccga agtgccgttt      300

ccgaccttca cggaaaccgg ctttccggaa gatctgatta cggaactgga aaaagcattc      360

gacaaccgca aaattatctg caataaagct ggctccggta ccgaaattat cgatcagtat      420

gcagaagaac agaaacaaaa cggttcaatt atcgtctaca cgagtaatga ctccgtgctg      480

cagattgcgg cccatgaaga atggatcggc ctggaaaacc tgtatcgtta cggtaaagca      540

gctcgcgaaa tttgtagttc caaaccggaa tggaatgtgg gtcgtattat cgtccgcccg      600

tttgtgggcg aagatggtaa atatacgcgt accttcaacc gccatgacta cgcgaatgcc      660

ccggaaccga tgattctgaa caaactgcag gaagcaggca tcgaagtcat cgctatcggt      720

aaaatcaacg atatcttcgt tggccaaggt attagccgtg cgatccactc atcgggcgat      780

gccgacggta tggataaagc aattgaactg gctcagctgg acgaagaaaa caaattcatc      840

ttcgtgaacc tggttcaatt cgatagccat tatggccacc gtcgcgatgt tgacggtttc      900

gcgtctaacg tcgccaccct ggacagtaaa ctgggcaaac tgattcaggt gatgaagaaa      960

gatgacctgc tgatcatgac ctcggatcat ggcaacgacc cggcctatcc gggttttaat     1020

cacacgcgtg aaatgctgcc gctgaccatc tactccaaac tgttcaaaga accgcgcgtt     1080

ctgaacgatg tcaccggcct gggtaccacg ggtaatattg ttgcgcgcaa ctttggcgtg     1140

gaaacggtta gcctgacggg cgaagacatt tttgataaac tggtgtga                  1188


<210>  20
<211>  395
<212>  PRT
<213>  Mycoplasma bovis

<400>  20

Met Ala Lys Phe Lys Arg Ile Phe Met Ile Val Thr Asp Gly Leu Gly 
1               5                   10                  15      


Ile Gly Pro Asp Lys Asp Gln Lys Lys Phe Gly Asp Ala Gly Ala Asn 
            20                  25                  30          


Thr Leu Tyr Ser Ala Ser Leu Ser Ser Met Phe Phe Ile Asp Thr Trp 
        35                  40                  45              


Lys Lys Leu Gly Ile Gly Asn Val Ala Gln Leu Glu Gly Asn Tyr His 
    50                  55                  60                  


Val Lys Ser Pro Leu Ala Tyr Val Ser Arg Ile Gln Glu Val Ser Asn 
65                  70                  75                  80  


Ala Lys Asp Thr Leu Ala Gly His Trp Glu Met Met Gly Ile Lys Thr 
                85                  90                  95      


Glu Val Pro Phe Pro Thr Phe Thr Glu Thr Gly Phe Pro Glu Asp Leu 
            100                 105                 110         


Ile Thr Glu Leu Glu Lys Ala Phe Asp Asn Arg Lys Ile Ile Cys Asn 
        115                 120                 125             


Lys Ala Gly Ser Gly Thr Glu Ile Ile Asp Gln Tyr Ala Glu Glu Gln 
    130                 135                 140                 


Lys Gln Asn Gly Ser Ile Ile Val Tyr Thr Ser Asn Asp Ser Val Leu 
145                 150                 155                 160 


Gln Ile Ala Ala His Glu Glu Trp Ile Gly Leu Glu Asn Leu Tyr Arg 
                165                 170                 175     


Tyr Gly Lys Ala Ala Arg Glu Ile Cys Ser Ser Lys Pro Glu Trp Asn 
            180                 185                 190         


Val Gly Arg Ile Ile Val Arg Pro Phe Val Gly Glu Asp Gly Lys Tyr 
        195                 200                 205             


Thr Arg Thr Phe Asn Arg His Asp Tyr Ala Asn Ala Pro Glu Pro Met 
    210                 215                 220                 


Ile Leu Asn Lys Leu Gln Glu Ala Gly Ile Glu Val Ile Ala Ile Gly 
225                 230                 235                 240 


Lys Ile Asn Asp Ile Phe Val Gly Gln Gly Ile Ser Arg Ala Ile His 
                245                 250                 255     


Ser Ser Gly Asp Ala Asp Gly Met Asp Lys Ala Ile Glu Leu Ala Gln 
            260                 265                 270         


Leu Asp Glu Glu Asn Lys Phe Ile Phe Val Asn Leu Val Gln Phe Asp 
        275                 280                 285             


Ser His Tyr Gly His Arg Arg Asp Val Asp Gly Phe Ala Ser Asn Val 
    290                 295                 300                 


Ala Thr Leu Asp Ser Lys Leu Gly Lys Leu Ile Gln Val Met Lys Lys 
305                 310                 315                 320 


Asp Asp Leu Leu Ile Met Thr Ser Asp His Gly Asn Asp Pro Ala Tyr 
                325                 330                 335     


Pro Gly Phe Asn His Thr Arg Glu Met Leu Pro Leu Thr Ile Tyr Ser 
            340                 345                 350         


Lys Leu Phe Lys Glu Pro Arg Val Leu Asn Asp Val Thr Gly Leu Gly 
        355                 360                 365             


Thr Thr Gly Asn Ile Val Ala Arg Asn Phe Gly Val Glu Thr Val Ser 
    370                 375                 380                 


Leu Thr Gly Glu Asp Ile Phe Asp Lys Leu Val 
385                 390                 395 


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide HH2

<400>  21

Val Gln Leu Arg Ile Arg Val Ala Val Ile Arg Ala 
1               5                   10          


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide 1002

<400>  22

Val Gln Arg Trp Leu Ile Val Trp Arg Ile Arg Lys 
1               5                   10          


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide 1018

<400>  23

Val Arg Leu Ile Val Ala Val Arg Ile Trp Arg Arg 
1               5                   10          


<210>  24
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide indolicidin

<400>  24

Ile Leu Pro Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  25
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide HH111

<400>  25

Ile Leu Lys Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  26
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide HH113

<400>  26

Ile Leu Pro Trp Lys Lys Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  27
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide HH970

<400>  27

Ile Leu Lys Trp Lys Trp Pro Trp Trp Lys Trp Arg Arg 
1               5                   10              


<210>  28
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide HH1010

<400>  28

Ile Leu Arg Trp Lys Trp Arg Trp Trp Arg Trp Arg Arg 
1               5                   10              


<210>  29
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide Nisin Z

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is Dhb

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is Dha

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa is Abu

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Xaa is Abu

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  Xaa is Abu

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Xaa is Abu

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa is Dha

<400>  29

Ile Xaa Ala Ile Xaa Leu Ala Xaa Pro Gly Ala Lys Xaa Gly Ala Leu 
1               5                   10                  15      


Met Gly Ala Asn Met Lys Xaa Ala Xaa Ala Asn Ala Ser Ile Asn Val 
            20                  25                  30          


Xaa Lys 
        

<210>  30
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK1

<400>  30

Val Phe Leu Arg Arg Ile Arg Val Ile Val Ile Arg 
1               5                   10          


<210>  31
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK2

<400>  31

Val Phe Trp Arg Arg Ile Arg Val Trp Val Ile Arg 
1               5                   10          


<210>  32
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK3

<400>  32

Val Gln Leu Arg Ala Ile Arg Val Arg Val Ile Arg 
1               5                   10          


<210>  33
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK4

<400>  33

Val Gln Leu Arg Arg Ile Arg Val Trp Val Ile Arg 
1               5                   10          


<210>  34
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK5

<400>  34

Val Gln Trp Arg Ala Ile Arg Val Arg Val Ile Arg 
1               5                   10          


<210>  35
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Host defence peptide JK6

<400>  35

Val Gln Trp Arg Arg Ile Arg Val Trp Val Ile Arg 
1               5                   10          


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG ODN 1826, a class B CpG, phosphorothioated

<400>  36
tccatgacgt tcctgacgtt                                                   20


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG ODN 2007, a class B CpG, phosphorothioated

<400>  37
tcgtcgttgt cgttttgtcg tt                                                22


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG 7907 or 10103, a class B CpG, phosphorothioated

<400>  38
tcgtcgtttt gtcgttttgt cgtt                                              24


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG 8954, a class A CpG

<400>  39
ggggacgacg tcgtgggggg g                                                 21


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG 2395 or CpG 10101, a class C CpG, phosphorothioated

<400>  40
tcgtcgtttt cggcgcgcgc cg                                                22


<210>  41
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a non-CpG oliogonucleotide

<400>  41
aaaaaaggta cctaaatagt atgtttctga aa                                     32


<210>  42
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  carboxy-terminus consensus amino acid sequence for RTX toxin

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa is Lys, Asp, Val, or Asn

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is Lys, Asp, Val, or Asn

<400>  42

Gly Gly Xaa Gly Xaa Asp 
1               5       
    


<210>  43
<211>  2778
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding leukotoxin 352 from plasmid pAA352

<400>  43
atggctactg ttatagatct aagcttccca aaaactgggg caaaaaaaat tatcctctat       60

attccccaaa attaccaata tgatactgaa caaggtaatg gtttacagga tttagtcaaa      120

gcggccgaag agttggggat tgaggtacaa agagaagaac gcaataatat tgcaacagct      180

caaaccagtt taggcacgat tcaaaccgct attggcttaa ctgagcgtgg cattgtgtta      240

tccgctccac aaattgataa attgctacag aaaactaaag caggccaagc attaggttct      300

gccgaaagca ttgtacaaaa tgcaaataaa gccaaaactg tattatctgg cattcaatct      360

attttaggct cagtattggc tggaatggat ttagatgagg ccttacagaa taacagcaac      420

caacatgctc ttgctaaagc tggcttggag ctaacaaatt cattaattga aaatattgct      480

aattcagtaa aaacacttga cgaatttggt gagcaaatta gtcaatttgg ttcaaaacta      540

caaaatatca aaggcttagg gactttagga gacaaactca aaaatatcgg tggacttgat      600

aaagctggcc ttggtttaga tgttatctca gggctattat cgggcgcaac agctgcactt      660

gtacttgcag ataaaaatgc ttcaacagct aaaaaagtgg gtgcgggttt tgaattggca      720

aaccaagttg ttggtaatat taccaaagcc gtttcttctt acattttagc ccaacgtgtt      780

gcagcaggtt tatcttcaac tgggcctgtg gctgctttaa ttgcttctac tgtttctctt      840

gcgattagcc cattagcatt tgccggtatt gccgataaat ttaatcatgc aaaaagttta      900

gagagttatg ccgaacgctt taaaaaatta ggctatgacg gagataattt attagcagaa      960

tatcagcggg gaacagggac tattgatgca tcggttactg caattaatac cgcattggcc     1020

gctattgctg gtggtgtgtc tgctgctgca gccggctcgg ttattgcttc accgattgcc     1080

ttattagtat ctgggattac cggtgtaatt tctacgattc tgcaatattc taaacaagca     1140

atgtttgagc acgttgcaaa taaaattcat aacaaaattg tagaatggga aaaaaataat     1200

cacggtaaga actactttga aaatggttac gatgcccgtt atcttgcgaa tttacaagat     1260

aatatgaaat tcttactgaa cttaaacaaa gagttacagg cagaacgtgt catcgctatt     1320

actcagcagc aatgggataa caacattggt gatttagctg gtattagccg tttaggtgaa     1380

aaagtcctta gtggtaaagc ctatgtggat gcgtttgaag aaggcaaaca cattaaagcc     1440

gataaattag tacagttgga ttcggcaaac ggtattattg atgtgagtaa ttcgggtaaa     1500

gcgaaaactc agcatatctt attcagaacg ccattattga cgccgggaac agagcatcgt     1560

gaacgcgtac aaacaggtaa atatgaatat attaccaagc tcaatattaa ccgtgtagat     1620

agctggaaaa ttacagatgg tgcagcaagt tctacctttg atttaactaa cgttgttcag     1680

cgtattggta ttgaattaga caatgctgga aatgtaacta aaaccaaaga aacaaaaatt     1740

attgccaaac ttggtgaagg tgatgacaac gtatttgttg gttctggtac gacggaaatt     1800

gatggcggtg aaggttacga ccgagttcac tatagccgtg gaaactatgg tgctttaact     1860

attgatgcaa ccaaagagac cgagcaaggt agttataccg taaatcgttt cgtagaaacc     1920

ggtaaagcac tacacgaagt gacttcaacc cataccgcat tagtgggcaa ccgtgaagaa     1980

aaaatagaat atcgtcatag caataaccag caccatgccg gttattacac caaagatacc     2040

ttgaaagctg ttgaagaaat tatcggtaca tcacataacg atatctttaa aggtagtaag     2100

ttcaatgatg cctttaacgg tggtgatggt gtcgatacta ttgacggtaa cgacggcaat     2160

gaccgcttat ttggtggtaa aggcgatgat attctcgatg gtggaaatgg tgatgatttt     2220

atcgatggcg gtaaaggcaa cgacctatta cacggtggca agggcgatga tattttcgtt     2280

caccgtaaag gcgatggtaa tgatattatt accgattctg acggcaatga taaattatca     2340

ttctctgatt cgaacttaaa agatttaaca tttgaaaaag ttaaacataa tcttgtcatc     2400

acgaatagca aaaaagagaa agtgaccatt caaaactggt tccgagaggc tgattttgct     2460

aaagaagtgc ctaattataa agcaactaaa gatgagaaaa tcgaagaaat catcggtcaa     2520

aatggcgagc ggatcacctc aaagcaagtt gatgatctta tcgcaaaagg taacggcaaa     2580

attacccaag atgagctatc aaaagttgtt gataactatg aattgctcaa acatagcaaa     2640

aatgtgacaa acagcttaga taagttaatc tcatctgtaa gtgcatttac ctcgtctaat     2700

gattcgagaa atgtattagt ggctccaact tcaatgttgg atcaaagttt atcttctctt     2760

caatttgcta ggggatcc                                                   2778


<210>  44
<211>  926
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of leukotoxin 352 from pasmid pAA352 with 
       flanking sequences from plasmid

<400>  44

Met Ala Thr Val Ile Asp Leu Ser Phe Pro Lys Thr Gly Ala Lys Lys 
1               5                   10                  15      


Ile Ile Leu Tyr Ile Pro Gln Asn Tyr Gln Tyr Asp Thr Glu Gln Gly 
            20                  25                  30          


Asn Gly Leu Gln Asp Leu Val Lys Ala Ala Glu Glu Leu Gly Ile Glu 
        35                  40                  45              


Val Gln Arg Glu Glu Arg Asn Asn Ile Ala Thr Ala Gln Thr Ser Leu 
    50                  55                  60                  


Gly Thr Ile Gln Thr Ala Ile Gly Leu Thr Glu Arg Gly Ile Val Leu 
65                  70                  75                  80  


Ser Ala Pro Gln Ile Asp Lys Leu Leu Gln Lys Thr Lys Ala Gly Gln 
                85                  90                  95      


Ala Leu Gly Ser Ala Glu Ser Ile Val Gln Asn Ala Asn Lys Ala Lys 
            100                 105                 110         


Thr Val Leu Ser Gly Ile Gln Ser Ile Leu Gly Ser Val Leu Ala Gly 
        115                 120                 125             


Met Asp Leu Asp Glu Ala Leu Gln Asn Asn Ser Asn Gln His Ala Leu 
    130                 135                 140                 


Ala Lys Ala Gly Leu Glu Leu Thr Asn Ser Leu Ile Glu Asn Ile Ala 
145                 150                 155                 160 


Asn Ser Val Lys Thr Leu Asp Glu Phe Gly Glu Gln Ile Ser Gln Phe 
                165                 170                 175     


Gly Ser Lys Leu Gln Asn Ile Lys Gly Leu Gly Thr Leu Gly Asp Lys 
            180                 185                 190         


Leu Lys Asn Ile Gly Gly Leu Asp Lys Ala Gly Leu Gly Leu Asp Val 
        195                 200                 205             


Ile Ser Gly Leu Leu Ser Gly Ala Thr Ala Ala Leu Val Leu Ala Asp 
    210                 215                 220                 


Lys Asn Ala Ser Thr Ala Lys Lys Val Gly Ala Gly Phe Glu Leu Ala 
225                 230                 235                 240 


Asn Gln Val Val Gly Asn Ile Thr Lys Ala Val Ser Ser Tyr Ile Leu 
                245                 250                 255     


Ala Gln Arg Val Ala Ala Gly Leu Ser Ser Thr Gly Pro Val Ala Ala 
            260                 265                 270         


Leu Ile Ala Ser Thr Val Ser Leu Ala Ile Ser Pro Leu Ala Phe Ala 
        275                 280                 285             


Gly Ile Ala Asp Lys Phe Asn His Ala Lys Ser Leu Glu Ser Tyr Ala 
    290                 295                 300                 


Glu Arg Phe Lys Lys Leu Gly Tyr Asp Gly Asp Asn Leu Leu Ala Glu 
305                 310                 315                 320 


Tyr Gln Arg Gly Thr Gly Thr Ile Asp Ala Ser Val Thr Ala Ile Asn 
                325                 330                 335     


Thr Ala Leu Ala Ala Ile Ala Gly Gly Val Ser Ala Ala Ala Ala Gly 
            340                 345                 350         


Ser Val Ile Ala Ser Pro Ile Ala Leu Leu Val Ser Gly Ile Thr Gly 
        355                 360                 365             


Val Ile Ser Thr Ile Leu Gln Tyr Ser Lys Gln Ala Met Phe Glu His 
    370                 375                 380                 


Val Ala Asn Lys Ile His Asn Lys Ile Val Glu Trp Glu Lys Asn Asn 
385                 390                 395                 400 


His Gly Lys Asn Tyr Phe Glu Asn Gly Tyr Asp Ala Arg Tyr Leu Ala 
                405                 410                 415     


Asn Leu Gln Asp Asn Met Lys Phe Leu Leu Asn Leu Asn Lys Glu Leu 
            420                 425                 430         


Gln Ala Glu Arg Val Ile Ala Ile Thr Gln Gln Gln Trp Asp Asn Asn 
        435                 440                 445             


Ile Gly Asp Leu Ala Gly Ile Ser Arg Leu Gly Glu Lys Val Leu Ser 
    450                 455                 460                 


Gly Lys Ala Tyr Val Asp Ala Phe Glu Glu Gly Lys His Ile Lys Ala 
465                 470                 475                 480 


Asp Lys Leu Val Gln Leu Asp Ser Ala Asn Gly Ile Ile Asp Val Ser 
                485                 490                 495     


Asn Ser Gly Lys Ala Lys Thr Gln His Ile Leu Phe Arg Thr Pro Leu 
            500                 505                 510         


Leu Thr Pro Gly Thr Glu His Arg Glu Arg Val Gln Thr Gly Lys Tyr 
        515                 520                 525             


Glu Tyr Ile Thr Lys Leu Asn Ile Asn Arg Val Asp Ser Trp Lys Ile 
    530                 535                 540                 


Thr Asp Gly Ala Ala Ser Ser Thr Phe Asp Leu Thr Asn Val Val Gln 
545                 550                 555                 560 


Arg Ile Gly Ile Glu Leu Asp Asn Ala Gly Asn Val Thr Lys Thr Lys 
                565                 570                 575     


Glu Thr Lys Ile Ile Ala Lys Leu Gly Glu Gly Asp Asp Asn Val Phe 
            580                 585                 590         


Val Gly Ser Gly Thr Thr Glu Ile Asp Gly Gly Glu Gly Tyr Asp Arg 
        595                 600                 605             


Val His Tyr Ser Arg Gly Asn Tyr Gly Ala Leu Thr Ile Asp Ala Thr 
    610                 615                 620                 


Lys Glu Thr Glu Gln Gly Ser Tyr Thr Val Asn Arg Phe Val Glu Thr 
625                 630                 635                 640 


Gly Lys Ala Leu His Glu Val Thr Ser Thr His Thr Ala Leu Val Gly 
                645                 650                 655     


Asn Arg Glu Glu Lys Ile Glu Tyr Arg His Ser Asn Asn Gln His His 
            660                 665                 670         


Ala Gly Tyr Tyr Thr Lys Asp Thr Leu Lys Ala Val Glu Glu Ile Ile 
        675                 680                 685             


Gly Thr Ser His Asn Asp Ile Phe Lys Gly Ser Lys Phe Asn Asp Ala 
    690                 695                 700                 


Phe Asn Gly Gly Asp Gly Val Asp Thr Ile Asp Gly Asn Asp Gly Asn 
705                 710                 715                 720 


Asp Arg Leu Phe Gly Gly Lys Gly Asp Asp Ile Leu Asp Gly Gly Asn 
                725                 730                 735     


Gly Asp Asp Phe Ile Asp Gly Gly Lys Gly Asn Asp Leu Leu His Gly 
            740                 745                 750         


Gly Lys Gly Asp Asp Ile Phe Val His Arg Lys Gly Asp Gly Asn Asp 
        755                 760                 765             


Ile Ile Thr Asp Ser Asp Gly Asn Asp Lys Leu Ser Phe Ser Asp Ser 
    770                 775                 780                 


Asn Leu Lys Asp Leu Thr Phe Glu Lys Val Lys His Asn Leu Val Ile 
785                 790                 795                 800 


Thr Asn Ser Lys Lys Glu Lys Val Thr Ile Gln Asn Trp Phe Arg Glu 
                805                 810                 815     


Ala Asp Phe Ala Lys Glu Val Pro Asn Tyr Lys Ala Thr Lys Asp Glu 
            820                 825                 830         


Lys Ile Glu Glu Ile Ile Gly Gln Asn Gly Glu Arg Ile Thr Ser Lys 
        835                 840                 845             


Gln Val Asp Asp Leu Ile Ala Lys Gly Asn Gly Lys Ile Thr Gln Asp 
    850                 855                 860                 


Glu Leu Ser Lys Val Val Asp Asn Tyr Glu Leu Leu Lys His Ser Lys 
865                 870                 875                 880 


Asn Val Thr Asn Ser Leu Asp Lys Leu Ile Ser Ser Val Ser Ala Phe 
                885                 890                 895     


Thr Ser Ser Asn Asp Ser Arg Asn Val Leu Val Ala Pro Thr Ser Met 
            900                 905                 910         


Leu Asp Gln Ser Leu Ser Ser Leu Gln Phe Ala Arg Gly Ser 
        915                 920                 925     


    


