                         SEQUENCE LISTING

<110>  BERG LLC
 
<120>  Enolase 1 (ENO1) compositions and uses thereof

<130>  119992-14920

<150>  62/193,582
<151>  2015-07-16

<150>  62/207,152
<151>  2015-08-19

<150>  62/235,584
<151>  2015-10-01

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  2204
<212>  DNA
<213>  Homo sapiens

<400>  1
gtggggcccc agagcgacgc tgagtgcgtg cgggactcgg agtacgtgac ggagccccga       60

gctctcatgc ccgccacgcc gccccgggcc atcccccgga gccccggctc cgcacacccc      120

agttcggctc accggtccta tctggggcca gagtttcgcc cgcaccacta cagggccgct      180

ggggagtcgg ggccccccag atctgcccgc ctcaagtccg cgggacgtca cccccctttc      240

cacgctactg cagccgtcgc agtcccaccc ctttccggga ggtgagggaa tgagtgacgg      300

ctctcccgac gaatggcgag gcggagctga gggggcgtgc cccggaggcg ggaagtgggt      360

ggggctcgcc ttagctaggc aggaagtcgg cgcgggcggc gcggacagta tctgtgggta      420

cccggagcac ggagatctcg ccggctttac gttcacctcg gtgtctgcag caccctccgc      480

ttcctctcct aggcgacgag acccagtggc tagaagttca ccatgtctat tctcaagatc      540

catgccaggg agatctttga ctctcgcggg aatcccactg ttgaggttga tctcttcacc      600

tcaaaaggtc tcttcagagc tgctgtgccc agtggtgctt caactggtat ctatgaggcc      660

ctagagctcc gggacaatga taagactcgc tatatgggga agggtgtctc aaaggctgtt      720

gagcacatca ataaaactat tgcgcctgcc ctggttagca agaaactgaa cgtcacagaa      780

caagagaaga ttgacaaact gatgatcgag atggatggaa cagaaaataa atctaagttt      840

ggtgcgaacg ccattctggg ggtgtccctt gccgtctgca aagctggtgc cgttgagaag      900

ggggtccccc tgtaccgcca catcgctgac ttggctggca actctgaagt catcctgcca      960

gtcccggcgt tcaatgtcat caatggcggt tctcatgctg gcaacaagct ggccatgcag     1020

gagttcatga tcctcccagt cggtgcagca aacttcaggg aagccatgcg cattggagca     1080

gaggtttacc acaacctgaa gaatgtcatc aaggagaaat atgggaaaga tgccaccaat     1140

gtgggggatg aaggcgggtt tgctcccaac atcctggaga ataaagaagg cctggagctg     1200

ctgaagactg ctattgggaa agctggctac actgataagg tggtcatcgg catggacgta     1260

gcggcctccg agttcttcag gtctgggaag tatgacctgg acttcaagtc tcccgatgac     1320

cccagcaggt acatctcgcc tgaccagctg gctgacctgt acaagtcctt catcaaggac     1380

tacccagtgg tgtctatcga agatcccttt gaccaggatg actggggagc ttggcagaag     1440

ttcacagcca gtgcaggaat ccaggtagtg ggggatgatc tcacagtgac caacccaaag     1500

aggatcgcca aggccgtgaa cgagaagtcc tgcaactgcc tcctgctcaa agtcaaccag     1560

attggctccg tgaccgagtc tcttcaggcg tgcaagctgg cccaggccaa tggttggggc     1620

gtcatggtgt ctcatcgttc gggggagact gaagatacct tcatcgctga cctggttgtg     1680

gggctgtgca ctgggcagat caagactggt gccccttgcc gatctgagcg cttggccaag     1740

tacaaccagc tcctcagaat tgaagaggag ctgggcagca aggctaagtt tgccggcagg     1800

aacttcagaa accccttggc caagtaagct gtgggcaggc aagcccttcg gtcacctgtt     1860

ggctacacag acccctcccc tcgtgtcagc tcaggcagct cgaggccccc gaccaacact     1920

tgcaggggtc cctgctagtt agcgccccac cgccgtggag ttcgtaccgc ttccttagaa     1980

cttctacaga agccaagctc cctggagccc tgttggcagc tctagctttg cagtcgtgta     2040

attggcccaa gtcattgttt ttctcgcctc actttccacc aagtgtctag agtcatgtga     2100

gcctcgtgtc atctccgggg tggccacagg ctagatcccc ggtggttttg tgctcaaaat     2160

aaaaagcctc agtgacccat gagaataaaa aaaaaaaaaa aaaa                      2204


<210>  2
<211>  434
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ser Ile Leu Lys Ile His Ala Arg Glu Ile Phe Asp Ser Arg Gly 
1               5                   10                  15      


Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser Lys Gly Leu Phe Arg 
            20                  25                  30          


Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile Tyr Glu Ala Leu Glu 
        35                  40                  45              


Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly Lys Gly Val Ser Lys 
    50                  55                  60                  


Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro Ala Leu Val Ser Lys 
65                  70                  75                  80  


Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp Lys Leu Met Ile Glu 
                85                  90                  95      


Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly Ala Asn Ala Ile Leu 
            100                 105                 110         


Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala Val Glu Lys Gly Val 
        115                 120                 125             


Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly Asn Ser Glu Val Ile 
    130                 135                 140                 


Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly Gly Ser His Ala Gly 
145                 150                 155                 160 


Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu Pro Val Gly Ala Ala 
                165                 170                 175     


Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu Val Tyr His Asn Leu 
            180                 185                 190         


Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp Ala Thr Asn Val Gly 
        195                 200                 205             


Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu Asn Lys Glu Gly Leu 
    210                 215                 220                 


Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly Tyr Thr Asp Lys Val 
225                 230                 235                 240 


Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe Phe Arg Ser Gly Lys 
                245                 250                 255     


Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro Ser Arg Tyr Ile Ser 
            260                 265                 270         


Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe Ile Lys Asp Tyr Pro 
        275                 280                 285             


Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp Asp Trp Gly Ala Trp 
    290                 295                 300                 


Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val Val Gly Asp Asp Leu 
305                 310                 315                 320 


Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala Val Asn Glu Lys Ser 
                325                 330                 335     


Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile Gly Ser Val Thr Glu 
            340                 345                 350         


Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn Gly Trp Gly Val Met 
        355                 360                 365             


Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr Phe Ile Ala Asp Leu 
    370                 375                 380                 


Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr Gly Ala Pro Cys Arg 
385                 390                 395                 400 


Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu Glu Glu 
                405                 410                 415     


Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn Phe Arg Asn Pro Leu 
            420                 425                 430         


Ala Lys 
        


<210>  3
<211>  2567
<212>  DNA
<213>  Homo sapiens

<400>  3
aactaaagaa aagtttcccc atctcccagg agggttctgt gggccctcca gagatcatca       60

gcctcttcac gggctagaaa ggatccaggg aaggtctaac caatgacctg ccctgaatgg      120

tgagctgcag gtgtgtcatt tagtgtgatt ttcctgttga ctgactcata ggagccctgc      180

tctgtggcag agctagcctc tggctgtatt caaattgact tagtgtgtgt gcaacattga      240

cctttctaga gatagaacat gtggccaaat tacagaaaag cacatagggc tagatcacgc      300

attctcagtg gggcacccgg aaaactccaa aaaggctgca gggaggggac aatgatgaaa      360

tcaggttgtg aaacactggg ctggtgtcgc agtggtggtg ctgggtgttc agtcccgctt      420

taatgctgta agaagcactc tacacacacg aacatgttac catttgaccg ttgtttaatg      480

gcgtacgtgg ggacttagcc ggagcaggat gatgctgtgc cttgatggta atgagtgctc      540

agtaagtaag catttgtgga agattgaacg catggcccct gaaatgctct cctctgcttt      600

cctgccccct cactgtctct cactcgcagt ccttaatcac cggttctctt ctgagtctct      660

ctcatttttc cttcttcatc ctctgctggg caggcgtctc cagacccatt aagtatatta      720

atgagttcct ggcaccagcc ctgtgcactc aggtaactga ttgaacagcc tttagtctgc      780

agttggcgtt tccagtgcat ggtcttgcaa actaacctcc agtcagatcg ttctgagcca      840

gctgctgttt tgtgtggctc taaccctctg gggtcctagg taggagcact cagactgggc      900

cggaaagtcc tccgattctg gggggaaagg ggagaggggg aagaggtccc acagaaggtc      960

ccttggtggg cttccgcgtc ggcctcaaca gtggttctct ctaacaatgc tgctcaagcc     1020

tgttttaaag ttaatgtcag taatttgatt tgattgttcc ttccaggtgt ctcaaaggct     1080

gttgagcaca tcaataaaac tattgcgcct gccctggtta gcaagaaact gaacgtcaca     1140

gaacaagaga agattgacaa actgatgatc gagatggatg gaacagaaaa taaatctaag     1200

tttggtgcga acgccattct gggggtgtcc cttgccgtct gcaaagctgg tgccgttgag     1260

aagggggtcc ccctgtaccg ccacatcgct gacttggctg gcaactctga agtcatcctg     1320

ccagtcccgg cgttcaatgt catcaatggc ggttctcatg ctggcaacaa gctggccatg     1380

caggagttca tgatcctccc agtcggtgca gcaaacttca gggaagccat gcgcattgga     1440

gcagaggttt accacaacct gaagaatgtc atcaaggaga aatatgggaa agatgccacc     1500

aatgtggggg atgaaggcgg gtttgctccc aacatcctgg agaataaaga aggcctggag     1560

ctgctgaaga ctgctattgg gaaagctggc tacactgata aggtggtcat cggcatggac     1620

gtagcggcct ccgagttctt caggtctggg aagtatgacc tggacttcaa gtctcccgat     1680

gaccccagca ggtacatctc gcctgaccag ctggctgacc tgtacaagtc cttcatcaag     1740

gactacccag tggtgtctat cgaagatccc tttgaccagg atgactgggg agcttggcag     1800

aagttcacag ccagtgcagg aatccaggta gtgggggatg atctcacagt gaccaaccca     1860

aagaggatcg ccaaggccgt gaacgagaag tcctgcaact gcctcctgct caaagtcaac     1920

cagattggct ccgtgaccga gtctcttcag gcgtgcaagc tggcccaggc caatggttgg     1980

ggcgtcatgg tgtctcatcg ttcgggggag actgaagata ccttcatcgc tgacctggtt     2040

gtggggctgt gcactgggca gatcaagact ggtgcccctt gccgatctga gcgcttggcc     2100

aagtacaacc agctcctcag aattgaagag gagctgggca gcaaggctaa gtttgccggc     2160

aggaacttca gaaacccctt ggccaagtaa gctgtgggca ggcaagccct tcggtcacct     2220

gttggctaca cagacccctc ccctcgtgtc agctcaggca gctcgaggcc cccgaccaac     2280

acttgcaggg gtccctgcta gttagcgccc caccgccgtg gagttcgtac cgcttcctta     2340

gaacttctac agaagccaag ctccctggag ccctgttggc agctctagct ttgcagtcgt     2400

gtaattggcc caagtcattg tttttctcgc ctcactttcc accaagtgtc tagagtcatg     2460

tgagcctcgt gtcatctccg gggtggccac aggctagatc cccggtggtt ttgtgctcaa     2520

aataaaaagc ctcagtgacc catgagaata aaaaaaaaaa aaaaaaa                   2567


<210>  4
<211>  341
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly Ala Asn 
1               5                   10                  15      


Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala Val Glu 
            20                  25                  30          


Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly Asn Ser 
        35                  40                  45              


Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly Gly Ser 
    50                  55                  60                  


His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu Pro Val 
65                  70                  75                  80  


Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu Val Tyr 
                85                  90                  95      


His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp Ala Thr 
            100                 105                 110         


Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu Asn Lys 
        115                 120                 125             


Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly Tyr Thr 
    130                 135                 140                 


Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe Phe Arg 
145                 150                 155                 160 


Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro Ser Arg 
                165                 170                 175     


Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe Ile Lys 
            180                 185                 190         


Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp Asp Trp 
        195                 200                 205             


Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val Val Gly 
    210                 215                 220                 


Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala Val Asn 
225                 230                 235                 240 


Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile Gly Ser 
                245                 250                 255     


Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn Gly Trp 
            260                 265                 270         


Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr Phe Ile 
        275                 280                 285             


Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr Gly Ala 
    290                 295                 300                 


Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu Arg Ile 
305                 310                 315                 320 


Glu Glu Glu Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn Phe Arg 
                325                 330                 335     


Asn Pro Leu Ala Lys 
            340     


<210>  5
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENO1 fusion protein

<400>  5

Met Ala Ser Ser Leu Asn Ile Ala Ser Ser Gly Val Asp Leu Gly Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Ser Ile Leu Lys Ile His Ala Arg Glu Ile 
            20                  25                  30          


Phe Asp Ser Arg Gly Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser 
        35                  40                  45              


Lys Gly Leu Phe Arg Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile 
    50                  55                  60                  


Tyr Glu Ala Leu Glu Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly 
65                  70                  75                  80  


Lys Gly Val Ser Lys Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro 
                85                  90                  95      


Ala Leu Val Ser Lys Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp 
            100                 105                 110         


Lys Leu Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly 
        115                 120                 125             


Ala Asn Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala 
    130                 135                 140                 


Val Glu Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly 
145                 150                 155                 160 


Asn Ser Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly 
                165                 170                 175     


Gly Ser His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu 
            180                 185                 190         


Pro Val Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu 
        195                 200                 205             


Val Tyr His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp 
    210                 215                 220                 


Ala Thr Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu 
225                 230                 235                 240 


Asn Lys Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly 
                245                 250                 255     


Tyr Thr Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe 
            260                 265                 270         


Phe Arg Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro 
        275                 280                 285             


Ser Arg Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe 
    290                 295                 300                 


Ile Lys Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp 
305                 310                 315                 320 


Asp Trp Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val 
                325                 330                 335     


Val Gly Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala 
            340                 345                 350         


Val Asn Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile 
        355                 360                 365             


Gly Ser Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn 
    370                 375                 380                 


Gly Trp Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr 
385                 390                 395                 400 


Phe Ile Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr 
                405                 410                 415     


Gly Ala Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu 
            420                 425                 430         


Arg Ile Glu Glu Glu Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn 
        435                 440                 445             


Phe Arg Asn Pro Leu Ala Lys 
    450                 455 


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  6

Ser Ser Gly Val Asp Leu Gly Thr Glu Asn Leu Tyr Phe Gln 
1               5                   10                  


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Muscle targeting peptide

<400>  7

Ala Ser Ser Leu Asn Ile Ala 
1               5           


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Muscle targeting peptide

<400>  8

Trp Asp Ala Asn Gly Lys Thr 
1               5           


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Muscle targeting peptide

<400>  9

Gly Glu Thr Arg Ala Pro Leu 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Muscle targeting peptide

<400>  10

Cys Gly His His Pro Val Tyr Ala Cys 
1               5                   


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Muscle targeting peptide

<400>  11

His Ala Ile Tyr Pro Arg His 
1               5           


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV protease cleavage site

<400>  12

Glu Asn Leu Tyr Phe Gln 
1               5       


<210>  13
<211>  433
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENO1 with N-terminal methionine removed

<400>  13

Ser Ile Leu Lys Ile His Ala Arg Glu Ile Phe Asp Ser Arg Gly Asn 
1               5                   10                  15      


Pro Thr Val Glu Val Asp Leu Phe Thr Ser Lys Gly Leu Phe Arg Ala 
            20                  25                  30          


Ala Val Pro Ser Gly Ala Ser Thr Gly Ile Tyr Glu Ala Leu Glu Leu 
        35                  40                  45              


Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly Lys Gly Val Ser Lys Ala 
    50                  55                  60                  


Val Glu His Ile Asn Lys Thr Ile Ala Pro Ala Leu Val Ser Lys Lys 
65                  70                  75                  80  


Leu Asn Val Thr Glu Gln Glu Lys Ile Asp Lys Leu Met Ile Glu Met 
                85                  90                  95      


Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly Ala Asn Ala Ile Leu Gly 
            100                 105                 110         


Val Ser Leu Ala Val Cys Lys Ala Gly Ala Val Glu Lys Gly Val Pro 
        115                 120                 125             


Leu Tyr Arg His Ile Ala Asp Leu Ala Gly Asn Ser Glu Val Ile Leu 
    130                 135                 140                 


Pro Val Pro Ala Phe Asn Val Ile Asn Gly Gly Ser His Ala Gly Asn 
145                 150                 155                 160 


Lys Leu Ala Met Gln Glu Phe Met Ile Leu Pro Val Gly Ala Ala Asn 
                165                 170                 175     


Phe Arg Glu Ala Met Arg Ile Gly Ala Glu Val Tyr His Asn Leu Lys 
            180                 185                 190         


Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp Ala Thr Asn Val Gly Asp 
        195                 200                 205             


Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu Asn Lys Glu Gly Leu Glu 
    210                 215                 220                 


Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly Tyr Thr Asp Lys Val Val 
225                 230                 235                 240 


Ile Gly Met Asp Val Ala Ala Ser Glu Phe Phe Arg Ser Gly Lys Tyr 
                245                 250                 255     


Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro Ser Arg Tyr Ile Ser Pro 
            260                 265                 270         


Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe Ile Lys Asp Tyr Pro Val 
        275                 280                 285             


Val Ser Ile Glu Asp Pro Phe Asp Gln Asp Asp Trp Gly Ala Trp Gln 
    290                 295                 300                 


Lys Phe Thr Ala Ser Ala Gly Ile Gln Val Val Gly Asp Asp Leu Thr 
305                 310                 315                 320 


Val Thr Asn Pro Lys Arg Ile Ala Lys Ala Val Asn Glu Lys Ser Cys 
                325                 330                 335     


Asn Cys Leu Leu Leu Lys Val Asn Gln Ile Gly Ser Val Thr Glu Ser 
            340                 345                 350         


Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn Gly Trp Gly Val Met Val 
        355                 360                 365             


Ser His Arg Ser Gly Glu Thr Glu Asp Thr Phe Ile Ala Asp Leu Val 
    370                 375                 380                 


Val Gly Leu Cys Thr Gly Gln Ile Lys Thr Gly Ala Pro Cys Arg Ser 
385                 390                 395                 400 


Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu Glu Glu Leu 
                405                 410                 415     


Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn Phe Arg Asn Pro Leu Ala 
            420                 425                 430         


Lys 
    


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  14

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10                  15  


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide added to C-terminus of ENO1

<400>  15

Gly Ile Glu Gly Arg 
1               5   


<210>  16
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S267C ENO1 fusion protein 

<400>  16

Met Ala Ser Ser Leu Asn Ile Ala Ser Ser Gly Val Asp Leu Gly Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Ser Ile Leu Lys Ile His Ala Arg Glu Ile 
            20                  25                  30          


Phe Asp Ser Arg Gly Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser 
        35                  40                  45              


Lys Gly Leu Phe Arg Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile 
    50                  55                  60                  


Tyr Glu Ala Leu Glu Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly 
65                  70                  75                  80  


Lys Gly Val Ser Lys Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro 
                85                  90                  95      


Ala Leu Val Ser Lys Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp 
            100                 105                 110         


Lys Leu Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly 
        115                 120                 125             


Ala Asn Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala 
    130                 135                 140                 


Val Glu Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly 
145                 150                 155                 160 


Asn Ser Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly 
                165                 170                 175     


Gly Ser His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu 
            180                 185                 190         


Pro Val Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu 
        195                 200                 205             


Val Tyr His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp 
    210                 215                 220                 


Ala Thr Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu 
225                 230                 235                 240 


Asn Lys Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly 
                245                 250                 255     


Tyr Thr Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe 
            260                 265                 270         


Phe Arg Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro 
        275                 280                 285             


Cys Arg Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe 
    290                 295                 300                 


Ile Lys Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp 
305                 310                 315                 320 


Asp Trp Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val 
                325                 330                 335     


Val Gly Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala 
            340                 345                 350         


Val Asn Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile 
        355                 360                 365             


Gly Ser Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn 
    370                 375                 380                 


Gly Trp Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr 
385                 390                 395                 400 


Phe Ile Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr 
                405                 410                 415     


Gly Ala Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu 
            420                 425                 430         


Arg Ile Glu Glu Glu Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn 
        435                 440                 445             


Phe Arg Asn Pro Leu Ala Lys Gly Ile Glu Gly Arg 
    450                 455                 460 


<210>  17
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S140C Eno1 fusion protein

<400>  17

Met Ala Ser Ser Leu Asn Ile Ala Ser Ser Gly Val Asp Leu Gly Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Ser Ile Leu Lys Ile His Ala Arg Glu Ile 
            20                  25                  30          


Phe Asp Ser Arg Gly Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser 
        35                  40                  45              


Lys Gly Leu Phe Arg Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile 
    50                  55                  60                  


Tyr Glu Ala Leu Glu Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly 
65                  70                  75                  80  


Lys Gly Val Ser Lys Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro 
                85                  90                  95      


Ala Leu Val Ser Lys Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp 
            100                 105                 110         


Lys Leu Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly 
        115                 120                 125             


Ala Asn Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala 
    130                 135                 140                 


Val Glu Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly 
145                 150                 155                 160 


Asn Cys Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly 
                165                 170                 175     


Gly Ser His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu 
            180                 185                 190         


Pro Val Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu 
        195                 200                 205             


Val Tyr His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp 
    210                 215                 220                 


Ala Thr Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu 
225                 230                 235                 240 


Asn Lys Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly 
                245                 250                 255     


Tyr Thr Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe 
            260                 265                 270         


Phe Arg Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro 
        275                 280                 285             


Ser Arg Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe 
    290                 295                 300                 


Ile Lys Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp 
305                 310                 315                 320 


Asp Trp Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val 
                325                 330                 335     


Val Gly Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala 
            340                 345                 350         


Val Asn Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile 
        355                 360                 365             


Gly Ser Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn 
    370                 375                 380                 


Gly Trp Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr 
385                 390                 395                 400 


Phe Ile Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr 
                405                 410                 415     


Gly Ala Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu 
            420                 425                 430         


Arg Ile Glu Glu Glu Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn 
        435                 440                 445             


Phe Arg Asn Pro Leu Ala Lys Gly Ile Glu Gly Arg 
    450                 455                 460 


<210>  18
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S418C Eno1 fusion protein

<400>  18

Met Ala Ser Ser Leu Asn Ile Ala Ser Ser Gly Val Asp Leu Gly Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Ser Ile Leu Lys Ile His Ala Arg Glu Ile 
            20                  25                  30          


Phe Asp Ser Arg Gly Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser 
        35                  40                  45              


Lys Gly Leu Phe Arg Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile 
    50                  55                  60                  


Tyr Glu Ala Leu Glu Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly 
65                  70                  75                  80  


Lys Gly Val Ser Lys Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro 
                85                  90                  95      


Ala Leu Val Ser Lys Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp 
            100                 105                 110         


Lys Leu Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly 
        115                 120                 125             


Ala Asn Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala 
    130                 135                 140                 


Val Glu Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly 
145                 150                 155                 160 


Asn Ser Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly 
                165                 170                 175     


Gly Ser His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu 
            180                 185                 190         


Pro Val Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu 
        195                 200                 205             


Val Tyr His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp 
    210                 215                 220                 


Ala Thr Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu 
225                 230                 235                 240 


Asn Lys Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly 
                245                 250                 255     


Tyr Thr Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe 
            260                 265                 270         


Phe Arg Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro 
        275                 280                 285             


Ser Arg Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe 
    290                 295                 300                 


Ile Lys Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp 
305                 310                 315                 320 


Asp Trp Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val 
                325                 330                 335     


Val Gly Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala 
            340                 345                 350         


Val Asn Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile 
        355                 360                 365             


Gly Ser Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn 
    370                 375                 380                 


Gly Trp Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr 
385                 390                 395                 400 


Phe Ile Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr 
                405                 410                 415     


Gly Ala Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu 
            420                 425                 430         


Arg Ile Glu Glu Glu Leu Gly Cys Lys Ala Lys Phe Ala Gly Arg Asn 
        435                 440                 445             


Phe Arg Asn Pro Leu Ala Lys Gly Ile Glu Gly Arg 
    450                 455                 460 


<210>  19
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S140C/S267C/S418C Eno1 fusion protein

<400>  19

Met Ala Ser Ser Leu Asn Ile Ala Ser Ser Gly Val Asp Leu Gly Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Ser Ile Leu Lys Ile His Ala Arg Glu Ile 
            20                  25                  30          


Phe Asp Ser Arg Gly Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser 
        35                  40                  45              


Lys Gly Leu Phe Arg Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile 
    50                  55                  60                  


Tyr Glu Ala Leu Glu Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly 
65                  70                  75                  80  


Lys Gly Val Ser Lys Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro 
                85                  90                  95      


Ala Leu Val Ser Lys Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp 
            100                 105                 110         


Lys Leu Met Ile Glu Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly 
        115                 120                 125             


Ala Asn Ala Ile Leu Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala 
    130                 135                 140                 


Val Glu Lys Gly Val Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly 
145                 150                 155                 160 


Asn Cys Glu Val Ile Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly 
                165                 170                 175     


Gly Ser His Ala Gly Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu 
            180                 185                 190         


Pro Val Gly Ala Ala Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu 
        195                 200                 205             


Val Tyr His Asn Leu Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp 
    210                 215                 220                 


Ala Thr Asn Val Gly Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu 
225                 230                 235                 240 


Asn Lys Glu Gly Leu Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly 
                245                 250                 255     


Tyr Thr Asp Lys Val Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe 
            260                 265                 270         


Phe Arg Ser Gly Lys Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro 
        275                 280                 285             


Cys Arg Tyr Ile Ser Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe 
    290                 295                 300                 


Ile Lys Asp Tyr Pro Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp 
305                 310                 315                 320 


Asp Trp Gly Ala Trp Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val 
                325                 330                 335     


Val Gly Asp Asp Leu Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala 
            340                 345                 350         


Val Asn Glu Lys Ser Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile 
        355                 360                 365             


Gly Ser Val Thr Glu Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn 
    370                 375                 380                 


Gly Trp Gly Val Met Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr 
385                 390                 395                 400 


Phe Ile Ala Asp Leu Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr 
                405                 410                 415     


Gly Ala Pro Cys Arg Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu 
            420                 425                 430         


Arg Ile Glu Glu Glu Leu Gly Cys Lys Ala Lys Phe Ala Gly Arg Asn 
        435                 440                 445             


Phe Arg Asn Pro Leu Ala Lys Gly Ile Glu Gly Arg 
    450                 455                 460 


<210>  20
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell penetrating peptide

<400>  20

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15      


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell penetrating peptide

<400>  21

Ala Gly Tyr Leu Leu Gly Lys Ile Asn Leu Lys Ala Leu Ala Ala Leu 
1               5                   10                  15      


Ala Lys Lys Ile Leu 
            20      


<210>  22
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell penetrating peptide

<400>  22

Met Val Thr Val Leu Phe Arg Arg Leu Arg Ile Arg Arg Ala Cys Gly 
1               5                   10                  15      


Pro Pro Arg Val Arg Val 
            20          


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell penetrating peptide

<400>  23

Tyr Asp Glu Glu Gly Gly Gly Glu 
1               5               


