                         SEQUENCE LISTING

<110>  NORTHWESTERN UNIVERSITY
 
<120>  IPSC-EC PERFORMANCE ENHANCEMENT VIA SIRT1 OVEREXPRESSION

<130>  NWEST-34531/WO-1/ORD

<150>  US 62/192,903
<151>  2015-07-15

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  555
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ile Gly Thr Asp Pro Arg Thr Ile Leu Lys Asp Leu Leu Pro Glu 
1               5                   10                  15      


Thr Ile Pro Pro Pro Glu Leu Asp Asp Met Thr Leu Trp Gln Ile Val 
            20                  25                  30          


Ile Asn Ile Leu Ser Glu Pro Pro Lys Arg Lys Lys Arg Lys Asp Ile 
        35                  40                  45              


Asn Thr Ile Glu Asp Ala Val Lys Leu Leu Gln Glu Cys Lys Lys Ile 
    50                  55                  60                  


Ile Val Leu Thr Gly Ala Gly Val Ser Val Ser Cys Gly Ile Pro Asp 
65                  70                  75                  80  


Phe Arg Ser Arg Asp Gly Ile Tyr Ala Arg Leu Ala Val Asp Phe Pro 
                85                  90                  95      


Asp Leu Pro Asp Pro Gln Ala Met Phe Asp Ile Glu Tyr Phe Arg Lys 
            100                 105                 110         


Asp Pro Arg Pro Phe Phe Lys Phe Ala Lys Glu Ile Tyr Pro Gly Gln 
        115                 120                 125             


Phe Gln Pro Ser Leu Cys His Lys Phe Ile Ala Leu Ser Asp Lys Glu 
    130                 135                 140                 


Gly Lys Leu Leu Arg Asn Tyr Thr Gln Asn Ile Asp Thr Leu Glu Gln 
145                 150                 155                 160 


Val Ala Gly Ile Gln Arg Ile Ile Gln Cys His Gly Ser Phe Ala Thr 
                165                 170                 175     


Ala Ser Cys Leu Ile Cys Lys Tyr Lys Val Asp Cys Glu Ala Val Arg 
            180                 185                 190         


Gly Ala Leu Phe Ser Gln Val Val Pro Arg Cys Pro Arg Cys Pro Ala 
        195                 200                 205             


Asp Glu Pro Leu Ala Ile Met Lys Pro Glu Ile Val Phe Phe Gly Glu 
    210                 215                 220                 


Asn Leu Pro Glu Gln Phe His Arg Ala Met Lys Tyr Asp Lys Asp Glu 
225                 230                 235                 240 


Val Asp Leu Leu Ile Val Ile Gly Ser Ser Leu Lys Val Arg Pro Val 
                245                 250                 255     


Ala Leu Ile Pro Ser Ser Ile Pro His Glu Val Pro Gln Ile Leu Ile 
            260                 265                 270         


Asn Arg Glu Pro Leu Pro His Leu His Phe Asp Val Glu Leu Leu Gly 
        275                 280                 285             


Asp Cys Asp Val Ile Ile Asn Glu Leu Cys His Arg Leu Gly Gly Glu 
    290                 295                 300                 


Tyr Ala Lys Leu Cys Cys Asn Pro Val Lys Leu Ser Glu Ile Thr Glu 
305                 310                 315                 320 


Lys Pro Pro Arg Thr Gln Lys Glu Leu Ala Tyr Leu Ser Glu Leu Pro 
                325                 330                 335     


Pro Thr Pro Leu His Val Ser Glu Asp Ser Ser Ser Pro Glu Arg Thr 
            340                 345                 350         


Ser Pro Pro Asp Ser Ser Val Ile Val Thr Leu Leu Asp Gln Ala Ala 
        355                 360                 365             


Lys Ser Asn Asp Asp Leu Asp Val Ser Glu Ser Lys Gly Cys Met Glu 
    370                 375                 380                 


Glu Lys Pro Gln Glu Val Gln Thr Ser Arg Asn Val Glu Ser Ile Ala 
385                 390                 395                 400 


Glu Gln Met Glu Asn Pro Asp Leu Lys Asn Val Gly Ser Ser Thr Gly 
                405                 410                 415     


Glu Lys Asn Glu Arg Thr Ser Val Ala Gly Thr Val Arg Lys Cys Trp 
            420                 425                 430         


Pro Asn Arg Val Ala Lys Glu Gln Ile Ser Arg Arg Leu Asp Gly Asn 
        435                 440                 445             


Gln Tyr Leu Phe Leu Pro Pro Asn Arg Tyr Ile Phe His Gly Ala Glu 
    450                 455                 460                 


Val Tyr Ser Asp Ser Glu Asp Asp Val Leu Ser Ser Ser Ser Cys Gly 
465                 470                 475                 480 


Ser Asn Ser Asp Ser Gly Thr Cys Gln Ser Pro Ser Leu Glu Glu Pro 
                485                 490                 495     


Met Glu Asp Glu Ser Glu Ile Glu Glu Phe Tyr Asn Gly Leu Glu Asp 
            500                 505                 510         


Glu Pro Asp Val Pro Glu Arg Ala Gly Gly Ala Gly Phe Gly Thr Asp 
        515                 520                 525             


Gly Asp Asp Gln Glu Ala Ile Asn Glu Ala Ile Ser Val Lys Gln Glu 
    530                 535                 540                 


Val Thr Asp Met Asn Tyr Pro Ser Asn Lys Ser 
545                 550                 555 


<210>  2
<211>  576
<212>  PRT
<213>  Mus musculus

<400>  2

Met Ala Ala Ala Ala Ala Ala Ala Ala Ile Gly Tyr Arg Gly Pro Tyr 
1               5                   10                  15      


Thr Phe Val Gln Gln His Leu Met Ile Gly Thr Asp Pro Arg Thr Ile 
            20                  25                  30          


Leu Lys Asp Leu Leu Pro Glu Thr Ile Pro Pro Pro Glu Leu Asp Asp 
        35                  40                  45              


Met Thr Leu Trp Gln Ile Val Ile Asn Ile Leu Ser Glu Pro Pro Lys 
    50                  55                  60                  


Arg Lys Lys Arg Lys Asp Ile Asn Thr Ile Glu Asp Ala Val Lys Leu 
65                  70                  75                  80  


Leu Gln Glu Cys Lys Lys Ile Ile Val Leu Thr Gly Ala Gly Val Ser 
                85                  90                  95      


Val Ser Cys Gly Ile Pro Asp Phe Arg Ser Arg Asp Gly Ile Tyr Ala 
            100                 105                 110         


Arg Leu Ala Val Asp Phe Pro Asp Leu Pro Asp Pro Gln Ala Met Phe 
        115                 120                 125             


Asp Ile Glu Tyr Phe Arg Lys Asp Pro Arg Pro Phe Phe Lys Phe Ala 
    130                 135                 140                 


Lys Glu Ile Tyr Pro Gly Gln Phe Gln Pro Ser Leu Cys His Lys Phe 
145                 150                 155                 160 


Ile Ala Leu Ser Asp Lys Glu Gly Lys Leu Leu Arg Asn Tyr Thr Gln 
                165                 170                 175     


Asn Ile Asp Thr Leu Glu Gln Val Ala Gly Ile Gln Arg Ile Leu Gln 
            180                 185                 190         


Cys His Gly Ser Phe Ala Thr Ala Ser Cys Leu Ile Cys Lys Tyr Lys 
        195                 200                 205             


Val Asp Cys Glu Ala Val Arg Gly Asp Ile Phe Asn Gln Val Val Pro 
    210                 215                 220                 


Arg Cys Pro Arg Cys Pro Ala Asp Glu Pro Leu Ala Ile Met Lys Pro 
225                 230                 235                 240 


Glu Ile Val Phe Phe Gly Glu Asn Leu Pro Glu Gln Phe His Arg Ala 
                245                 250                 255     


Met Lys Tyr Asp Lys Asp Glu Val Asp Leu Leu Ile Val Ile Gly Ser 
            260                 265                 270         


Ser Leu Lys Val Arg Pro Val Ala Leu Ile Pro Ser Ser Ile Pro His 
        275                 280                 285             


Glu Val Pro Gln Ile Leu Ile Asn Arg Glu Pro Leu Pro His Leu His 
    290                 295                 300                 


Phe Asp Val Glu Leu Leu Gly Asp Cys Asp Val Ile Ile Asn Glu Leu 
305                 310                 315                 320 


Cys His Arg Leu Gly Gly Glu Tyr Ala Lys Leu Cys Cys Asn Pro Val 
                325                 330                 335     


Lys Leu Ser Glu Ile Thr Glu Lys Pro Pro Arg Pro Gln Lys Glu Leu 
            340                 345                 350         


Val His Leu Ser Glu Leu Pro Pro Thr Pro Leu His Ile Ser Glu Asp 
        355                 360                 365             


Ser Ser Ser Pro Glu Arg Thr Val Pro Gln Asp Ser Ser Val Ile Ala 
    370                 375                 380                 


Thr Leu Val Asp Gln Ala Thr Asn Asn Asn Val Asn Asp Leu Glu Val 
385                 390                 395                 400 


Ser Glu Ser Ser Cys Val Glu Glu Lys Pro Gln Glu Val Gln Thr Ser 
                405                 410                 415     


Arg Asn Val Glu Asn Ile Asn Val Glu Asn Pro Asp Phe Lys Ala Val 
            420                 425                 430         


Gly Ser Ser Thr Ala Asp Lys Asn Glu Arg Thr Ser Val Ala Glu Thr 
        435                 440                 445             


Val Arg Lys Cys Trp Pro Asn Arg Leu Ala Lys Glu Gln Ile Ser Lys 
    450                 455                 460                 


Arg Leu Glu Gly Asn Gln Tyr Leu Phe Val Pro Pro Asn Arg Tyr Ile 
465                 470                 475                 480 


Phe His Gly Ala Glu Val Tyr Ser Asp Ser Glu Asp Asp Val Leu Ser 
                485                 490                 495     


Ser Ser Ser Cys Gly Ser Asn Ser Asp Ser Gly Thr Cys Gln Ser Pro 
            500                 505                 510         


Ser Leu Glu Glu Pro Leu Glu Asp Glu Ser Glu Ile Glu Glu Phe Tyr 
        515                 520                 525             


Asn Gly Leu Glu Asp Asp Thr Glu Arg Pro Glu Cys Ala Gly Gly Ser 
    530                 535                 540                 


Gly Phe Gly Ala Asp Gly Gly Asp Gln Glu Val Val Asn Glu Ala Ile 
545                 550                 555                 560 


Ala Thr Arg Gln Glu Leu Thr Asp Val Asn Tyr Pro Ser Asp Lys Ser 
                565                 570                 575     


<210>  3
<211>  5420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  plasmid DNA

<400>  3
agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatcga       60

cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg      120

agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc      180

acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc      240

cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg      300

gtgatgccgg ccacgatgcg tccggcgtag aggatccaga catgataaga tacattgatg      360

agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg      420

atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt      480

gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa      540

acctctacag atgtgatatg gctgattatg atcattactt atctaggtat aggctgcgca      600

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg      660

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta      720

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgaat ttagcggccg      780

cgaattcgcc cttgtctaga gtggaacaat tcctgtacct gcacaattat tacactatga      840

tttgtttgat ggatagttca tgtctgttac ttcctgtttc acagatatag cttcattaat      900

tgcctcttga tcatctccat cagtcccaaa tccagctcct ccagctctct ctggaacatc      960

aggctcatct tctaagccat tgtagaattc ttcaatttca ctttcatcct ccatgggttc     1020

ttctaaactt ggactctggc atgtcccact atcactgtta ctgccacaag aactagagga     1080

taagacgtca tcttcagagt ctgaatatac ctcagcgcca tggaaaatgt aacgatttgg     1140

tggcaaaaac agatactgat taccatcaag ccgcctacta atctgctcct ttgccactct     1200

attaggccag cattttctca ctgttccagc cactgaagtt ctttcatttt tctccccagt     1260

actagaacca acattcttca aatccggatt ttccatctgt tcagcaatac tttcaacatt     1320

cctagaagtt tgtacttcct gtggtttttc ttccatacaa ccttttgatt cagacacatc     1380

taaatcatca ttactcttag ctgcttggtc taaaagtgtg acaatcactg aagaatctgg     1440

tggtgaagtt ctttctggtg aacttgagtc ttctgaaaca tgaagaggtg tgggtggcaa     1500

ctctgacaaa taagccaatt ctttttgtgt tcgtggaggt ttttcagtaa tttctgaaag     1560

ctttacaggg ttacagcaaa gtttggcata ttcaccacct aacctatgac acaattcatt     1620

aattatgaca tcacagtctc caagaagctc tacatcaaaa tgcagatgag gcaaaggttc     1680

tctattaatt aatatctgag gcacttcatg gggtatggaa cttggaatta gtgctactgg     1740

tcttactttg agggaagacc caataacaat gaggaggtca acttcatctt tgtcatactt     1800

catggctcta tgaaactgtt ctggtaaatt ttcaccaaaa aacacaatct ctggtttcat     1860

gatagcaagc ggttcatcag ctgggcacct aggacatcga ggaactacct gattaaaaat     1920

atctcctcgt acagcttcac agtcaacttt gtatttacaa atcaggcaag atgctgttgc     1980

aaaggaacca tgacactgaa ttatcctttg gattcccgca acctgttcca gcgtgtctat     2040

gttctgggta tagttgcgaa gtagttttcc ttccttatct gacaaggcta tgaatttgtg     2100

acagagagat ggctggaatt gtccaggata tatttccttt gcaaacttga agaatggtct     2160

tggatctttt ctgaaatatt caatatcaaa catcgcttga ggatctggaa gatctgggaa     2220

gtctacagca aggcgagcat aaataccatc ccttgacctg aagtcaggta ttccacatga     2280

aacagacacc ccagctccag ttagaactat aatttttttg cactcttgca gtaatttcac     2340

agcatcttca attgtattaa tatcttttct ttttttcctt tttggtggtt ctgaaaggat     2400

attaataaca atctgccaca gtgtcatatc atccaactca ggtggaggta ttgtttccgg     2460

caataaatct ttaagaattg ttcgaggatc tgtgccaatc ataagatgtt gctgaacaaa     2520

agtatatgga cctatccgtg gccttggagt ccagtcacta gagcttgcat gtgaggctct     2580

atcctcctca tcactttcac aggaatgaaa accattagtg ataatttcat caccgaacag     2640

aaggttatct cggtacccaa tcgccgccgc cgccgcctct tcctcctcct cgccctcgtc     2700

gtcgtcgtct tcgtcgtaca agttgtcggc cagcggtggc tcccgagatg ggccctgcag     2760

gcccggccca ttgtctcctt ccccagccgc cgcagtcgcc tgggcctctt gctccccgcc     2820

tgccgccgcc gcctctgcct ccgcctcccg ccacagcgcc gccgccgccg cacccgggca     2880

gcccctggcc gccgccggca cctcacgctc tggggccgcc ccaccgggct cgcccgggct     2940

ccgctcgagg ccgggaccat ctctccgcgg cctcttgcgg agcggctccc cggcggggga     3000

cgacgcggcc tccctgtcgg cccccgccgc cgagggggag ccgccgggct gaagggcgag     3060

ggccgcctcg tccgccgaat tcccgggttt atcgtcatcg tccttgtagt ccatcctgag     3120

gatccgagct cggtaccaag cttagatctc ctccaaaaaa gcctcttcac tacttctgga     3180

atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat     3240

ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg     3300

ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc     3360

ctggggactt tccttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg     3420

gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct     3480

cagaaggtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt     3540

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     3600

atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt     3660

tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc     3720

tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat     3780

ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct     3840

atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca     3900

ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg     3960

catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa     4020

cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg     4080

ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga     4140

cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg     4200

cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt     4260

tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg     4320

agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc     4380

ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca     4440

gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc     4500

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     4560

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     4620

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     4680

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     4740

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct     4800

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     4860

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     4920

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     4980

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     5040

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     5100

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     5160

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     5220

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     5280

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat     5340

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc     5400

agtgagcgag gaagcggaag                                                 5420


