                         SEQUENCE LISTING

<110>  Winthrop University Hospotal
 
<120>  SYSTEM, METHOD AND KIT FOR ANALYSIS OF CIRCULATING DIFFERENTIALLY
        METHYLATED DNA AS A BIOMARKER OF BETA-CELL LOSS

<130>  PCT/2526-187

<150>  62/191,115
<151>  2015-07-10

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer for Mouse Amylin Analysis, first step PCR

<400>  1
tggtagtaat ttttagatgg ataaa                                             25


<210>  2
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer for Mouse Amylin Analysis, first step PCR

<400>  2
aaattcccta tttaaatccc ctac                                              24


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT mouse amylin, 
       hypermeth-specific forward primer

<400>  3
aaacggaagt gtaatacggt tac                                               23


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT mouse amylin, 
       hypermeth-specific reverse primer

<400>  4
ttaccatata tattcgatcc cacg                                              24


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT mouse amylin, 
       hypometh-specific forward primer

<400>  5
aaatggaagt gtaatatggt tat                                               23


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT mouse amylin, 
       hypometh-specific reverse primer

<400>  6
ttaccatata tattcaatcc caca                                              24


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer for Human Amylin Analysis, first step PCR

<400>  7
tgttattagt tattaggtgg aaaag                                             25


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer for Human Amylin Analysis, first step PCR

<400>  8
tcttaccata tatattaaat cccac                                             25


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT human amylin, common 
       forward primer

<400>  9
tgttattagt tattaggtgg aaaag                                             25


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT human amylin, 
       hypermeth-specific reverse primer

<400>  10
taaaaaattt accaaacgct acg                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hypermethylation-specific nested qRTPCT human amylin, 
       hypometh-specific reverse primer

<400>  11
taaaaaattt accaaacact aca                                               23


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Native Amylin PCR human forward primer

<400>  12
tgttaccagt catcaggtgg aaaag                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Native Amylin PCR human reverse primer

<400>  13
tcttgccata tgtattggat cccac                                             25


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human insulin methylation independent forward primer

<400>  14
gtgcggttta tatttggtgg aagtt                                             25


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human insulin methylation independent reverse primer

<400>  15
acaacaataa acaattaact caccctacaa                                        30


<210>  16
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human methylated insulin probe 1

<400>  16
acctcccgac gaatct                                                       16


<210>  17
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human methylated insulin probe 2

<400>  17
tacctctcgt cgaatct                                                      17


<210>  18
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human insulin demethylated DNA probe 3

<400>  18
acctcccaac aaatct                                                       16


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human insulin demethylated DNA probe 4

<400>  19
tacctcccat caaatct                                                      17


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification insulin DNA PCR forward primer (Akirav 
       2011)

<400>  20
ttaggggttt taaggtaggg tatttggt                                          28


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification insulin DNA PCR reverse primer (Akirav 
       2011)

<400>  21
accaaaaaca acaataaaca attaactcac cctacaa                                37


<210>  22
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification methylated insulin DNA PCR forward inner 
       primer

<400>  22
gtggatgcgt tttttgtttt tgttgg                                            26


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification methylated insulin DNA PCR reverse inner 
       primer

<400>  23
caccctacaa atcctctacc tcccg                                             25


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification demethylated insulin DNA PCR forward 
       inner primer

<400>  24
ttgtggatgt gttttttgtt tttgttggt                                         29


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer PCR amplification demethylated insulin DNA PCR reverse 
       inner primer

<400>  25
caccctacaa atcctctacc tccca                                             25


