                         SEQUENCE LISTING

<110>  INSTITUT PASTEUR
       ELIGO BIOSCIENCE
 
<120>  IMPROVING SEQUENCE-SPECIFIC ANTIMICROBIALS BY BLOCKING DNA REPAIR

<130>  B370726D36399

<150>  US62/191,572
<151>  2015-07-13

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  1
gtttttgtac tctcaagatt taagtaactg tacaac                                 36


<210>  2
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  2
gatataaacc taattacctc gagaggggac ggaaac                                 36


<210>  3
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  3
gttttggaac cattcgaaac aacacagctc taaaac                                 36


<210>  4
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  4
gttttagagc tatgctgttt tgaatggtcc caaaac                                 36


<210>  5
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  5
atttcaatcc actcacccat gaagggtgag ac                                     32


<210>  6
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  6
gtttcagtag ctagattatt tgatatactg ctgttag                                37


<210>  7
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  7
aatcagagaa taccccgtat aaaaggggac gagaac                                 36


<210>  8
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  8
gttcactgcc gcacaggcag cttagaaa                                          28


<210>  9
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  9
ggttgtagct ccctttctca tttcgcagtg ctacaat                                37


<210>  10
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  repeat sequence

<400>  10
ccggattccc gcctgcgcgg gaatgacg                                          28


<210>  11
<211>  5120
<212>  DNA
<213>  Artificial

<220>
<223>  pLC13

<400>  11
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac       60

tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca      120

ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta      180

aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata      240

ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag      300

cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag      360

caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg      420

tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct      480

tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc      540

ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc      600

gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca      660

tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga      720

tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa      780

acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata      840

taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc      900

ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt      960

tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat     1020

tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta     1080

accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt     1140

aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca     1200

ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta     1260

tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt     1320

aactttaaga aggagatata ccatggctaa accagcaaaa cgtatcaaga gtgccgcagc     1380

ggcttatgtg ccacaaaacc gcgatgcggt gattaccgat attaaacgca tcggggattt     1440

acagcgcgaa gcatcacgtc tggaaacgga aatgaatgat gccatcgcgg aaattacgga     1500

gaaatttgcg gcccggattg caccgattaa aaccgatatt gaaacccttt caaaaggcgt     1560

tcagggatgg tgtgaagcga accgcgacga actgacgaac ggcggcaaag tgaagacggc     1620

gaatcttgtc accggtgatg tatcgtggcg ggtccgtcca ccatcagtaa gtattcgtgg     1680

tatggatgca gtgatggaaa cgctggagcg tcttggcctg caacgcttta ttcgcacgaa     1740

gcaggaaatc aacaaggaag cgattttact ggaaccgaaa gcggtcgcag gcgttgccgg     1800

aattacagtt aaatcaggca ttgaggattt ttctattatt ccatttgaac aggaagccgg     1860

tatttaattg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca     1920

gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca     1980

cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct     2040

ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga     2100

ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc     2160

gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc     2220

gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc     2280

gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     2340

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac     2400

atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc     2460

cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca     2520

tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc     2580

caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg     2640

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac     2700

cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca     2760

taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg     2820

agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac     2880

cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg     2940

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat     3000

taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg     3060

ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg     3120

cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc     3180

aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc     3240

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt     3300

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     3360

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     3420

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     3480

cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga     3540

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     3600

caaacttgaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg     3660

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     3720

aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct     3780

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa     3840

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     3900

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     3960

gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta     4020

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct     4080

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg     4140

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag     4200

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc     4260

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg     4320

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt     4380

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg     4440

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca     4500

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg     4560

agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc     4620

ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat     4680

atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg     4740

ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg     4800

ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg     4860

agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcaaggagat     4920

ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct     4980

catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc     5040

agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg     5100

ctcatgtttg acagcttatc                                                 5120


<210>  12
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC2

<400>  12
ccttcttaaa gttaccgagc tcgaattcgc                                        30


<210>  13
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC296

<400>  13
tatattttag gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt       60


<210>  14
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC397

<400>  14
atccgccaaa acagccaatt aaataccggc ttcctgttc                              39


<210>  15
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC398

<400>  15
gcgaattcga gctcggtaac tttaagaagg agatatacca tggctaaacc agcaaaacgt       60

a                                                                       61


<210>  16
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer LacZ1

<400>  16
tcactggccg tcgttttaca acgtcgtgac                                        30


<210>  17
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer LacZ2

<400>  17
ccattacggt caatccgccg tttgttccca                                        30


<210>  18
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer rpsL

<400>  18
tactttacgc agcgcggagt tcggtttttt                                        30


<210>  19
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer mhpR

<400>  19
ggaattaatc gaaatgttag cctcccgccc                                        30


<210>  20
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer tsuB

<400>  20
taaggtcttc gttcagggca tagaccttaa                                        30


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer wcaH

<400>  21
ttttctcgct gagaagcgta ccggagtacc                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer irhA

<400>  22
attccgctgc gcagtaccag tgtgttggcg                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer eamB

<400>  23
cagcggtaca ccttttgagt tgggcggggg                                        30


<210>  24
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer speA

<400>  24
agcagaacgt ctgaatgtcg ttcctcgtct                                        30


<210>  25
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer garD

<400>  25
cgtggtgggg ctgaatcatt tgtacggttg                                        30


<210>  26
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer treF

<400>  26
gtaccgcgat ttacgcgcgg gggcggcctc                                        30


<210>  27
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer yfaP

<400>  27
attcgtgcac gtttacggct ggttctctcg                                        30


<210>  28
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacer ada

<400>  28
ggtgcgttac gcgctggctg attgtgagct                                        30


<210>  29
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  primer B299

<400>  29
catgaattca actcaacaag tctcagtgtg ctg                                    33


<210>  30
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC34

<400>  30
tttaggcgct gccatcttaa gacgaaaggg cctcgtgata                             40


<210>  31
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC35

<400>  31
ttcagcacac tgagacttgt tgagttgaat tcatgagtat taagtattgt tttatggctg       60

ata                                                                     63


<210>  32
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC36

<400>  32
tatcacgagg ccctttcgtc ttaagatggc agcgcctaaa                             40


<210>  33
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC41

<400>  33
tgcagcgcga tcgtaatcag gatcccatgg tacgcgt                                37


<210>  34
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC42

<400>  34
acagaactta atgggcccga agacgaaagg gcctcgt                                37


<210>  35
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC37

<400>  35
tccgccgttt gttcccacgt agaatccgac gggttgttac                             40


<210>  36
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC38

<400>  36
gtaacaaccc gtcggattct acgtgggaac aaacggcgga                             40


<210>  37
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC39

<400>  37
acgaggccct ttcgtcttcg ggcccattaa gttctgt                                37


<210>  38
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC40

<400>  38
acgcgtacca tgggatcctg attacgatcg cgctgca                                37


<210>  39
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC191

<400>  39
gtctagggcg gcggatttg                                                    19


<210>  40
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC192

<400>  40
cgctctcctg agtaggacaa at                                                22


<210>  41
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC193

<400>  41
acaattgaat accgatcggc ctcgtgatac gcctat                                 36


<210>  42
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC194

<400>  42
ataggcgtat cacgaggccg atcggtattc aattgtgccc aa                          42


<210>  43
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC195

<400>  43
caggggctgg attgattatg agtaaaggag aagaactttt c                           41


<210>  44
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC196

<400>  44
ttcttctcct ttactcataa tcaatccagc ccctgtga                               38


<210>  45
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC95

<400>  45
ctccgacgcc gaacccatac aacctcctta gtacatcaag ca                          42


<210>  46
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC96

<400>  46
gcaggacgcc cgccataaac tgccaggaat tggggatcgg ggggttccgc gcacattt         58


<210>  47
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC94

<400>  47
gcaggacgcc cgccataaac tgccaggaat tggggatcgg ggggttccgc gcacattt         58


<210>  48
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC98

<400>  48
agtttaggtt aggcgccatg catctcgagg catgcctgca tcattcgcgc accacctca        59


<210>  49
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC93

<400>  49
cgtccaaatg gctcgcatac aacctcctta gtacatcaag ca                          42


<210>  50
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC92

<400>  50
tgcttgatgt actaaggagg ttgtatgcga gccatttgga cg                          42


<210>  51
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC97

<400>  51
agtttaggtt aggcgccatg catctcgagg catgcctgca tcacggaggc gttgggac         58


<210>  52
<211>  64
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC100

<400>  52
gcaggacgcc cgccataaac tgccaggaat tggggatcgg ttaagaccca ctttcacatt       60

taag                                                                    64


<210>  53
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC101

<400>  53
agtttaggtt aggcgccatg catctcgagg catgcctgca tataaacgca gaaaggccc        59


<210>  54
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC33

<400>  54
gactggaaag cgggcagt                                                     18


<210>  55
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC47

<400>  55
cgcacgatag agattcggga                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC80

<400>  56
tcaggcggga tgaagatgat                                                   20


<210>  57
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC153

<400>  57
gctgggatac gctggtgttt a                                                 21


<210>  58
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC154

<400>  58
cacagcgcaa ggacgttga                                                    19


<210>  59
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer LC155

<400>  59
acacaacatg acgggctt                                                     18


<210>  60
<211>  9326
<212>  DNA
<213>  Artificial

<220>
<223>  pCas9 (pCas9-a)


<220>
<221>  gene
<222>  (219)..(8886)
<223>  gene cat - positions are given on the complementary strand : 
       complement (8886..219)

<220>
<221>  promoter
<222>  (220)..(322)
<223>  cat promoter - positions are given on the complementary strand : 
       complement (220..322)

<220>
<221>  rep_origin
<222>  (848)..(1393)
<223>  p15A ori - positions are given on the complementary strand : 
       complement (848..1393)

<220>
<221>  promoter
<222>  (1505)..(1533)
<223>  tet promoter

<220>
<221>  misc_feature
<222>  (1844)..(2014)
<223>  tracrRNA sequence of S. p (Zhangfeng) - positions are given on 
       the complementary strand : complement (1844..2014)

<220>
<221>  misc_feature
<222>  (1846)..(1932)
<223>  tracrRNA sequence of S. p (Zhangfeng)

<220>
<221>  gene
<222>  (2225)..(6331)
<223>  Cas9 (Csn1) endonuclease from the streptococcus pyogenes Type II 
       CRISPR/Cas system

<220>
<221>  misc_feature
<222>  (6484)..(6519)
<223>  repeat

<220>
<221>  misc_feature
<222>  (6550)..(6585)
<223>  repeat

<400>  60
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct     1560

aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg     1620

tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg     1680

attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag     1740

aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag     1800

cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc     1860

ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg     1920

aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa     1980

gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact     2040

ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga     2100

agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa     2160

ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc     2220

aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc     2280

ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga     2340

ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc     2400

ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat     2460

ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca     2520

tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt     2580

tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg     2640

aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc     2700

gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag     2760

tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa     2820

ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc     2880

aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg     2940

gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc     3000

agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt     3060

ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc     3120

tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc     3180

ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt     3240

tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata     3300

tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat     3360

tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct     3420

gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct     3480

gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa     3540

gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa     3600

tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga     3660

agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga     3720

taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac     3780

ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt     3840

tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt     3900

aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga     3960

aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa     4020

aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat     4080

tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata     4140

tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg     4200

gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat     4260

attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga     4320

tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag     4380

tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca     4440

gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat     4500

cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga     4560

gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca     4620

tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg     4680

aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga     4740

tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg     4800

ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat     4860

gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa     4920

tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg     4980

ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat     5040

gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa     5100

atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa     5160

caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa     5220

gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg     5280

taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta     5340

ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa     5400

acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga     5460

ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga     5520

agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct     5580

tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt     5640

agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc     5700

cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat     5760

tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc     5820

taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga     5880

attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc     5940

tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt     6000

ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg     6060

tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga     6120

caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg     6180

agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac     6240

aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg     6300

cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta     6360

ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga     6420

ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac     6480

gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc tcggaagctc     6540

aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga     6600

cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc     6660

gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt     6720

taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag     6780

catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc     6840

acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct     6900

acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta     6960

cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat     7020

cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt     7080

cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca     7140

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     7200

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     7260

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     7320

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     7380

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     7440

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     7500

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg     7560

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt     7620

gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct     7680

cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc     7740

cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt     7800

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc     7860

cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg     7920

agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc     7980

agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc     8040

gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat     8100

gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga     8160

gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc     8220

cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata     8280

ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc     8340

gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt     8400

atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc     8460

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc     8520

cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg     8580

aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta     8640

ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca     8700

cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac     8760

gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg     8820

cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa     8880

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     8940

ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc     9000

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     9060

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     9120

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     9180

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     9240

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     9300

agctcaccgt ctttcattgc catacg                                          9326


<210>  61
<211>  9326
<212>  DNA
<213>  Artificial

<220>
<223>  pCas9_LacZ2


<220>
<221>  gene
<222>  (2225)..(6331)
<223>  product

<220>
<221>  misc_feature
<222>  (6484)..(6519)
<223>  repeat

<220>
<221>  misc_feature
<222>  (6520)..(6549)
<223>  CRSIPR target ELZ2

<220>
<221>  misc_feature
<222>  (6550)..(6585)
<223>  repeat

<400>  61
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct     1560

aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg     1620

tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg     1680

attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag     1740

aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag     1800

cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc     1860

ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg     1920

aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa     1980

gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact     2040

ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga     2100

agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa     2160

ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc     2220

aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc     2280

ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga     2340

ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc     2400

ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat     2460

ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca     2520

tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt     2580

tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg     2640

aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc     2700

gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag     2760

tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa     2820

ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc     2880

aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg     2940

gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc     3000

agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt     3060

ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc     3120

tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc     3180

ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt     3240

tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata     3300

tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat     3360

tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct     3420

gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct     3480

gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa     3540

gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa     3600

tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga     3660

agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga     3720

taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac     3780

ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt     3840

tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt     3900

aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga     3960

aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa     4020

aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat     4080

tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata     4140

tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg     4200

gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat     4260

attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga     4320

tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag     4380

tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca     4440

gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat     4500

cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga     4560

gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca     4620

tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg     4680

aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga     4740

tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg     4800

ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat     4860

gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa     4920

tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg     4980

ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat     5040

gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa     5100

atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa     5160

caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa     5220

gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg     5280

taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta     5340

ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa     5400

acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga     5460

ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga     5520

agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct     5580

tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt     5640

agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc     5700

cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat     5760

tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc     5820

taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga     5880

attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc     5940

tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt     6000

ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg     6060

tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga     6120

caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg     6180

agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac     6240

aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg     6300

cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta     6360

ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga     6420

ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac     6480

gaggttttag agctatgctg ttttgaatgg tcccaaaacc cattacggtc aatccgccgt     6540

ttgttcccag ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga     6600

cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc     6660

gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt     6720

taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag     6780

catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc     6840

acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct     6900

acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta     6960

cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat     7020

cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt     7080

cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca     7140

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     7200

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     7260

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     7320

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     7380

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     7440

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     7500

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg     7560

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt     7620

gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct     7680

cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc     7740

cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt     7800

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc     7860

cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg     7920

agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc     7980

agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc     8040

gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat     8100

gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga     8160

gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc     8220

cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata     8280

ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc     8340

gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt     8400

atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc     8460

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc     8520

cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg     8580

aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta     8640

ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca     8700

cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac     8760

gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg     8820

cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa     8880

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     8940

ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc     9000

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     9060

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     9120

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     9180

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     9240

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     9300

agctcaccgt ctttcattgc catacg                                          9326


<210>  62
<211>  2349
<212>  DNA
<213>  Artificial

<220>
<223>  psgRNAc BsaI


<220>
<221>  gene
<222>  (219)..(1909)
<223>  cat CmR chloramphenicol acetyltransferase -  positions are given 
       on the complementary strand : complement (1909-219)

<220>
<221>  promoter
<222>  (220)..(322)
<223>  cat promoter -  positions are given on the complementary strand :
       complement (220..322)

<220>
<221>  rep_origin
<222>  (848)..(1393)
<223>  p15A ori  - positions are given on the complementary strand : 
       complement (848..1393)

<220>
<221>  promoter
<222>  (1510)..(1538)
<223>  Promoter (BBa_J23119)

<220>
<221>  misc_feature
<222>  (1545)..(1564)
<223>  Control spacer with 2 BsaI

<220>
<221>  misc_RNA
<222>  (1565)..(1640)
<223>  gRNA scaffold

<220>
<221>  misc_feature
<222>  (1642)..(1647)
<223>  part of the tracrRNA

<220>
<221>  gene
<222>  (1648)..(1652)
<223>  lacI

<400>  62
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctggcta ggaggtgact gaagtatatt ttaggaattc     1500

taaagatctt tgacagctag ctcagtccta ggtataatac tagttgagac cagtctaggt     1560

ctcggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa     1620

aagtggcacc gagtcggtgc tttttttggt agtgcagcgc gatcgtaatc aggggggaga     1680

gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc     1740

aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg     1800

acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc     1860

aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc     1920

ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc     1980

acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata     2040

atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc     2100

aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc     2160

tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag     2220

aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc     2280

atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat     2340

tgccatacg                                                             2349


<210>  63
<211>  9326
<212>  DNA
<213>  Artificial

<220>
<223>  pCas9

<400>  63
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct     1560

aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg     1620

tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg     1680

attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag     1740

aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag     1800

cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc     1860

ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg     1920

aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa     1980

gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact     2040

ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga     2100

agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa     2160

ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc     2220

aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc     2280

ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga     2340

ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc     2400

ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat     2460

ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca     2520

tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt     2580

tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg     2640

aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc     2700

gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag     2760

tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa     2820

ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc     2880

aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg     2940

gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc     3000

agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt     3060

ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc     3120

tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc     3180

ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt     3240

tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata     3300

tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat     3360

tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct     3420

gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct     3480

gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa     3540

gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa     3600

tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga     3660

agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga     3720

taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac     3780

ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt     3840

tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt     3900

aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga     3960

aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa     4020

aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat     4080

tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata     4140

tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg     4200

gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat     4260

attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga     4320

tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag     4380

tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca     4440

gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat     4500

cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga     4560

gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca     4620

tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg     4680

aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga     4740

tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg     4800

ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat     4860

gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa     4920

tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg     4980

ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat     5040

gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa     5100

atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa     5160

caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa     5220

gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg     5280

taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta     5340

ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa     5400

acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga     5460

ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga     5520

agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct     5580

tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt     5640

agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc     5700

cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat     5760

tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc     5820

taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga     5880

attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc     5940

tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt     6000

ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg     6060

tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga     6120

caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg     6180

agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac     6240

aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg     6300

cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta     6360

ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga     6420

ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac     6480

gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc tcggaagctc     6540

aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga     6600

cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc     6660

gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt     6720

taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag     6780

catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc     6840

acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct     6900

acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta     6960

cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat     7020

cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt     7080

cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca     7140

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     7200

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     7260

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     7320

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     7380

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     7440

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     7500

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg     7560

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt     7620

gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct     7680

cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc     7740

cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt     7800

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc     7860

cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg     7920

agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc     7980

agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc     8040

gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat     8100

gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga     8160

gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc     8220

cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata     8280

ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc     8340

gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt     8400

atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc     8460

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc     8520

cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg     8580

aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta     8640

ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca     8700

cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac     8760

gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg     8820

cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa     8880

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     8940

ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc     9000

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     9060

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     9120

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     9180

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     9240

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     9300

agctcaccgt ctttcattgc catacg                                          9326


<210>  64
<211>  2433
<212>  DNA
<213>  Artificial

<220>
<223>  pCRISPR

<400>  64
ctcgagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac       60

atcagcagga cgcactgacc gaattcaact caacaagtct cagtgtgctg aagttttggg      120

accattcaaa acagcatagc tctaaaacga gacctttgag cttccgagac tggtctcagt      180

tttgggacca ttcaaaacag catagctcta aaacctcgta gactattttt gtctaaaaaa      240

tttcgtaatc gcactatttg tctcagctag acttcagtct tgaaaagccc ctgtattact      300

gcatttatta agagtattat accatatttt tagttattaa gaaataggat cccatggtac      360

gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt      420

atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag      480

ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca      540

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac      600

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac      660

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg      720

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac      780

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat      840

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag      900

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac      960

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     1020

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt     1080

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     1140

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     1200

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     1260

gaaaactcac gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa     1320

cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg     1380

atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca gaagaactcg     1440

tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg     1500

aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct     1560

atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg     1620

ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg     1680

ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc     1740

tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg     1800

atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc     1860

cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga     1920

tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg     1980

agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc     2040

tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc     2100

gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag     2160

ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc     2220

atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc     2280

cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc     2340

gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa     2400

aataggcgta tcacgaggcc ctttcgtctt cac                                  2433


<210>  65
<211>  2650
<212>  DNA
<213>  Artificial

<220>
<223>  pCRRNA

<400>  65
aagatggcag cgcctaaatg tagaatgata aaaggattaa gagattaatt tccctaaaaa       60

tgataaaaca agcgttttga aagcgcttgt ttttttggtt tgcagtcaga gtagaataga      120

agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta      180

ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat tattttataa cttttataac      240

aaataatcaa ggagaaattc aaagaaattt atcagccata aaacaatact taatactcat      300

gaattcaact caacaagtct cagtgtgctg aagttttggg accattcaaa acagcatagc      360

tctaaaacga gacctttgag cttccgagac tggtctcagt tttgggacca ttcaaaacag      420

catagctcta aaacctcgta gactattttt gtctaaaaaa tttcgtaatc gcactatttg      480

tctcagctag acttcagtct tgaaaagccc ctgtattact gcatttatta agagtattat      540

accatatttt tagttattaa gaaataggat cccatggtac gcgtgctaga ggcatcaaat      600

aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa      660

cgctctcctg agtaggacaa atccgccgcc ctagacctag ggcgttcggc tgcggcgagc      720

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg      780

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct      840

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca      900

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct      960

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     1020

gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     1080

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     1140

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     1200

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     1260

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc     1320

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     1380

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     1440

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     1500

tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg     1560

ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa     1620

gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc     1680

gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc     1740

gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc     1800

cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt     1860

cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt     1920

gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg     1980

atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg     2040

gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat     2100

ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc     2160

caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac     2220

gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc     2280

ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc     2340

ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca     2400

agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc     2460

tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat     2520

ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga     2580

cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc     2640

ctttcgtctt                                                            2650


<210>  66
<211>  4085
<212>  DNA
<213>  Artificial

<220>
<223>  pLCX

<400>  66
taaaaatagg cgtatcacga ggccctttcg tcttcgggcc cattaagttc tgtctcggcg       60

cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg      120

gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat      180

gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg      240

cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac      300

gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc      360

ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag      420

ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg      480

caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc      540

cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc      600

accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata      660

acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac      720

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc      780

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc      840

gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa      900

gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga      960

tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc     1020

cgtttgttcc cacgtagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa     1080

gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc     1140

tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg     1200

acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga     1260

gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg     1320

tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta     1380

atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg     1440

actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg     1500

cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac     1560

gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg     1620

tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt     1680

tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc     1740

gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga     1800

tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt     1860

atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg     1920

aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct     1980

ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcaggatc     2040

ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc     2100

tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc     2160

tagacctagg gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     2220

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     2280

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     2340

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     2400

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     2460

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc     2520

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     2580

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     2640

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     2700

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca     2760

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     2820

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     2880

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     2940

cagtggaacg aaaactcacg ttaagggatt ttggtcatga ctagtgcttg gattctcacc     3000

aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt     3060

cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag     3120

aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg     3180

taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta     3240

gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca     3300

gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg     3360

agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc     3420

ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt     3480

gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta     3540

tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat     3600

gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg     3660

acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct     3720

gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg     3780

cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc     3840

cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct     3900

tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc     3960

catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta     4020

ccagagggcg ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta     4080

accta                                                                 4085


<210>  67
<211>  2349
<212>  DNA
<213>  Artificial

<220>
<223>  psgRNAc

<400>  67
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctggcta ggaggtgact gaagtatatt ttaggaattc     1500

taaagatctt tgacagctag ctcagtccta ggtataatac tagttgagac cagtctaggt     1560

ctcggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa     1620

aagtggcacc gagtcggtgc tttttttggt agtgcagcgc gatcgtaatc aggggggaga     1680

gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc     1740

aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg     1800

acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc     1860

aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc     1920

ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc     1980

acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata     2040

atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc     2100

aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc     2160

tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag     2220

aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc     2280

atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat     2340

tgccatacg                                                             2349


<210>  68
<211>  8701
<212>  DNA
<213>  Artificial

<220>
<223>  pPh1F-Cas9

<400>  68
tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag       60

ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc      120

aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt      180

acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg      240

gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa      300

cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa      360

atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt      420

ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt      480

tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc      540

aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg      600

caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt      660

ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat      720

cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat      780

ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat      840

cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc      900

atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa      960

ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact     1020

agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact     1080

tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta     1140

tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga     1200

tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc     1260

gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac     1320

cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg     1380

accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct     1440

ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa     1500

ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg     1560

ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc     1620

tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc     1680

agcggtatca tcaacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     1740

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     1800

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttacgttt ccacaaccaa     1860

ttaaccaatt ctgatttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat     1920

atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc     1980

accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc     2040

aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc     2100

accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac     2160

ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt     2220

attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt     2280

acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc     2340

acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt     2400

gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa     2460

ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt     2520

gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc     2580

acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt     2640

ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct     2700

tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg     2760

tgcaatgtaa catcagagat tttgagacac aacgtggctt tccctgcagg atttcggagg     2820

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2880

gctcgccgca gccgaacgcc ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt     2940

ttcagagggt attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta     3000

aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg     3060

atcaccggaa aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgtg     3120

gagggactag tggattttac ggctagctca gtcctaggta caatgctagc gaattcatta     3180

aagaggagaa aggtacccat ggcacgtacc ccgagccgta gcagcattgg tagcctgcgt     3240

agtccgcata cccataaagc aattctgacc agcaccattg aaatcctgaa agaatgtggt     3300

tatagcggtc tgagcattga aagcgttgca cgtcgtgccg gtgcaagcaa accgaccatt     3360

tatcgttggt ggaccaataa agcagcactg attgccgaag tgtatgaaaa tgaaagcgaa     3420

caggtgcgta aatttccgga tctgggtagc tttaaagccg atctggattt tctgctgcgt     3480

aatctgtgga aagtttggcg tgaaaccatt tgtggtgaag catttcgttg tgttattgca     3540

gaagcacagc tggaccctgc aaccctgacc cagctgaaag atcagtttat ggaacgtcgt     3600

cgtgagatgc cgaaaaaact ggttgaaaat gccattagca atggtgaact gccgaaagat     3660

accaatcgtg aactgctgct ggatatgatt tttggttttt gttggtatcg cctgctgacc     3720

gaacagctga ccgttgaaca ggatattgaa gaatttacct tcctgctgat taatggtgtt     3780

tgtccgggta cacagcgtta actagggccc atacccccaa ttattgaagg ccgctaacgc     3840

ggcctttttt tgtttctggt ctgcccgacg tacggtgaat ctgattcgtt accaattgac     3900

atgatacgaa acgtaccgta tcgttaaggt tactagagat taaagaggag aaatactaga     3960

tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga     4020

tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc     4080

acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag     4140

cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt     4200

atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac     4260

ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa     4320

atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa     4380

aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata     4440

tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg     4500

tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta     4560

ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac     4620

gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc     4680

tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag     4740

atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc     4800

aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt     4860

tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa     4920

tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac     4980

aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag     5040

gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag     5100

aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca     5160

agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg     5220

ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg     5280

aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc     5340

gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag     5400

ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa     5460

atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt     5520

ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt     5580

caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg     5640

ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt     5700

caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta     5760

ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt     5820

taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc     5880

acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac     5940

gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag     6000

attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata     6060

gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac     6120

atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg     6180

taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta     6240

ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta     6300

tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg     6360

ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag     6420

acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca     6480

ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg     6540

ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa     6600

actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa     6660

cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat     6720

tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata     6780

ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta     6840

aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt     6900

accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat     6960

atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa     7020

tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta     7080

atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc     7140

ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg     7200

ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac     7260

agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg     7320

ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt     7380

attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta     7440

aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact     7500

ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat     7560

atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac     7620

aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc     7680

attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc     7740

agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta     7800

ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac     7860

caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc     7920

ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag     7980

aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg     8040

atttgagtca gctaggaggt gactaactcg agtaaggatc tccaggcatt gcaggcatgc     8100

ctcgagatgc atggcgccta acctaaactg atgacgcatc ctcacgataa tatccgggta     8160

ggcgcaatca ctttcgtcta ctccgttaca aagcgaggct gggtatttcc cggcctttct     8220

gttatccgaa atccactgaa agcacagcgg ctggctgagg agataaataa taaacgaggg     8280

gctgtatgca caaagcatct tctgttgagt taagaacgag tatcgagatg gcacatagcc     8340

ttgctcaaat tggaatcagg tttgtgccaa taccagtaga aacagacgaa gaatccatgg     8400

gtatggacag atctcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga     8460

ctagccttat tttaacttgc tatttctagc tctaaaacgg gttttcccag tcacgacgtg     8520

ctagcattat acctaggact gagctagctg tcagccattc gatggtgtca acgtaaatgc     8580

atgccgctcg ccttccatgg gtatggacag ttttcccttt gatatgtaac ggtgaacagt     8640

tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac     8700

c                                                                     8701


<210>  69
<211>  4083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBAD-MuGam


<220>
<221>  misc_feature
<222>  (2566)..(2595)
<223>  n is a, c, g, or t

<400>  69
aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt       60

tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc      120

cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta      180

tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg      240

tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact      300

gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac      360

tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa      420

aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct      480

cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg      540

cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca      600

aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat      660

cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc      720

ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg      780

cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga      840

ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct      900

tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt      960

tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg     1020

gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct     1080

tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac     1140

caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc     1200

aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtatcgat     1260

gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc     1320

aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact     1380

ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc     1440

cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc     1500

cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag     1560

acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca     1620

tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga     1680

caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg     1740

cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc     1800

ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca     1860

tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag     1920

taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga     1980

ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat     2040

tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct     2100

ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt     2160

aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct     2220

tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc     2280

agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg     2340

cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa     2400

agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc     2460

tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa     2520

ctctctactg tttctccata cccgtttttt tgggctagcg aattcnnnnn nnnnnnnnnn     2580

nnnnnnnnnn nnnnnatggc taaaccagca aaacgtatca agagtgccgc agcggcttat     2640

gtgccacaaa accgcgatgc ggtgattacc gatattaaac gcatcgggga tttacagcgc     2700

gaagcatcac gtctggaaac ggaaatgaat gatgccatcg cggaaattac ggagaaattt     2760

gcggcccgga ttgcaccgat taaaaccgat attgaaaccc tttcaaaagg cgttcaggga     2820

tggtgtgaag cgaaccgcga cgaactgacg aacggcggca aagtgaagac ggcgaatctt     2880

gtcaccggtg atgtatcgtg gcgggtccgt ccaccatcag taagtattcg tggtatggat     2940

gcagtgatgg aaacgctgga gcgtcttggc ctgcaacgct ttattcgcac gaagcaggaa     3000

atcaacaagg aagcgatttt actggaaccg aaagcggtcg caggcgttgc cggaattaca     3060

gttaaatcag gcattgagga tttttctatt attccatttg aacaggaagc cggtatttaa     3120

taattttccc gccctcaaaa aagcaataaa gcggctcaat agccgcttta ttcacatcag     3180

caaaaattat atcgggtagc accagaagca cacggtcaca ctgcttccgg tagtcaataa     3240

accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac     3300

cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg     3360

tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc     3420

actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga     3480

cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt     3540

tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac     3600

tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag     3660

ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact     3720

gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga     3780

aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca     3840

tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa     3900

acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct     3960

ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt     4020

gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc     4080

ctg                                                                   4083


<210>  70
<211>  3979
<212>  DNA
<213>  artificial

<220>
<223>  pBAD-LambdaGam


<220>
<221>  misc_feature
<222>  (2570)..(2599)
<223>  n is a, c, g, or t

<400>  70
aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt       60

tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc      120

cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta      180

tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg      240

tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact      300

gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac      360

tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa      420

aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct      480

cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg      540

cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca      600

aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat      660

cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc      720

ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg      780

cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga      840

ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct      900

tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt      960

tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg     1020

gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct     1080

tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac     1140

caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc     1200

aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtatcgat     1260

gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc     1320

aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact     1380

ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc     1440

cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc     1500

cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag     1560

acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca     1620

tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga     1680

caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg     1740

cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc     1800

ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca     1860

tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag     1920

taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga     1980

ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat     2040

tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct     2100

ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt     2160

aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct     2220

tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc     2280

agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg     2340

cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa     2400

agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc     2460

tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa     2520

ctctctactg tttctccata cccgtttttt tgggctagcg aattcgagcn nnnnnnnnnn     2580

nnnnnnnnnn nnnnnnnnna tggatattaa tactgaaact gagatcaagc aaaagcattc     2640

actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag     2700

ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag     2760

ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga     2820

catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg     2880

ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga     2940

gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat     3000

tgattcagag gtataataat tttcccgccc tcaaaaaagc aataaagcgg ctcaatagcc     3060

gctttattca catcagcaaa aattatatcg ggtagcacca gaagcacacg gtcacactgc     3120

ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct     3180

gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt     3240

atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat     3300

tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca     3360

tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg     3420

ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc     3480

acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc     3540

tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa     3600

tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt     3660

tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca     3720

ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga     3780

ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata     3840

tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt     3900

tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt     3960

ttagcttcct tagctcctg                                                  3979


<210>  71
<211>  10701
<212>  DNA
<213>  Artificial

<220>
<223>  pCas9-MuGam


<220>
<221>  misc_feature
<222>  (9946)..(9976)
<223>  n is a, c, g, or t

<400>  71
tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag       60

ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc      120

aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt      180

acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg      240

gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa      300

cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa      360

atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt      420

ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt      480

tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc      540

aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg      600

caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt      660

ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat      720

cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat      780

ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat      840

cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc      900

atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa      960

ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact     1020

agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact     1080

tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta     1140

tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga     1200

tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc     1260

gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac     1320

cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg     1380

accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct     1440

ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa     1500

ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg     1560

ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc     1620

tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc     1680

agcggtatca tcaacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     1740

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     1800

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttacgttt ccacaaccaa     1860

ttaaccaatt ctgatttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat     1920

atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc     1980

accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc     2040

aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc     2100

accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac     2160

ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt     2220

attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt     2280

acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc     2340

acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt     2400

gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa     2460

ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt     2520

gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc     2580

acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt     2640

ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct     2700

tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg     2760

tgcaatgtaa catcagagat tttgagacac aacgtggctt tccctgcagg atttcggagg     2820

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2880

gctcgccgca gccgaacgcc ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt     2940

ttcagagggt attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta     3000

aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg     3060

atcaccggaa aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgtg     3120

gagggactag tggattttac ggctagctca gtcctaggta caatgctagc gaattcatta     3180

aagaggagaa aggtacccat ggcacgtacc ccgagccgta gcagcattgg tagcctgcgt     3240

agtccgcata cccataaagc aattctgacc agcaccattg aaatcctgaa agaatgtggt     3300

tatagcggtc tgagcattga aagcgttgca cgtcgtgccg gtgcaagcaa accgaccatt     3360

tatcgttggt ggaccaataa agcagcactg attgccgaag tgtatgaaaa tgaaagcgaa     3420

caggtgcgta aatttccgga tctgggtagc tttaaagccg atctggattt tctgctgcgt     3480

aatctgtgga aagtttggcg tgaaaccatt tgtggtgaag catttcgttg tgttattgca     3540

gaagcacagc tggaccctgc aaccctgacc cagctgaaag atcagtttat ggaacgtcgt     3600

cgtgagatgc cgaaaaaact ggttgaaaat gccattagca atggtgaact gccgaaagat     3660

accaatcgtg aactgctgct ggatatgatt tttggttttt gttggtatcg cctgctgacc     3720

gaacagctga ccgttgaaca ggatattgaa gaatttacct tcctgctgat taatggtgtt     3780

tgtccgggta cacagcgtta actagggccc atacccccaa ttattgaagg ccgctaacgc     3840

ggcctttttt tgtttctggt ctgcccgacg tacggtgaat ctgattcgtt accaattgac     3900

atgatacgaa acgtaccgta tcgttaaggt tactagagat taaagaggag aaatactaga     3960

tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga     4020

tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc     4080

acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag     4140

cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt     4200

atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac     4260

ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa     4320

atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa     4380

aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata     4440

tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg     4500

tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta     4560

ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac     4620

gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc     4680

tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag     4740

atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc     4800

aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt     4860

tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa     4920

tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac     4980

aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag     5040

gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag     5100

aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca     5160

agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg     5220

ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg     5280

aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc     5340

gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag     5400

ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa     5460

atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt     5520

ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt     5580

caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg     5640

ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt     5700

caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta     5760

ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt     5820

taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc     5880

acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac     5940

gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag     6000

attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata     6060

gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac     6120

atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg     6180

taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta     6240

ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta     6300

tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg     6360

ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag     6420

acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca     6480

ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg     6540

ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa     6600

actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa     6660

cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat     6720

tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata     6780

ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta     6840

aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt     6900

accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat     6960

atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa     7020

tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta     7080

atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc     7140

ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg     7200

ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac     7260

agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg     7320

ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt     7380

attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta     7440

aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact     7500

ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat     7560

atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac     7620

aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc     7680

attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc     7740

agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta     7800

ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac     7860

caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc     7920

ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag     7980

aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg     8040

atttgagtca gctaggaggt gactaactcg agtaaggatc tccaggcatt gcaggcatgc     8100

ctcgagatgc atggcgccta acctaaactg atgacgcatc ctcacgataa tatccgggta     8160

ggcgcaatca ctttcgtcta ctccgttaca aagcgaggct gggtatttcc cggcctttct     8220

gttatccgaa atccactgaa agcacagcgg ctggctgagg agataaataa taaacgaggg     8280

gctgtatgca caaagcatct tctgttgagt taagaacgag tatcgagatg gcacatagcc     8340

ttgctcaaat tggaatcagg tttgtgccaa taccagtaga aacagacgaa gaatccatgg     8400

gtatggacag atctcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga     8460

ctagccttat tttaacttgc tatttctagc tctaaaacgg gttttcccag tcacgacgtg     8520

ctagcattat acctaggact gagctagctg tcagccattc gatggtgtca acgtaaatgc     8580

atgccgcttc gcctcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat     8640

gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc     8700

aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact     8760

ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc     8820

cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc     8880

cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag     8940

acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca     9000

tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga     9060

caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg     9120

cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc     9180

ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca     9240

tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag     9300

taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga     9360

ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat     9420

tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct     9480

ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt     9540

aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct     9600

tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc     9660

agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg     9720

cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa     9780

agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc     9840

tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa     9900

ctctctactg tttctccata cccgtttttt tgggctagcg aattcnnnnn nnnnnnnnnn     9960

nnnnnnnnnn nnnnnnatgg ctaaaccagc aaaacgtatc aagagtgccg cagcggctta    10020

tgtgccacaa aaccgcgatg cggtgattac cgatattaaa cgcatcgggg atttacagcg    10080

cgaagcatca cgtctggaaa cggaaatgaa tgatgccatc gcggaaatta cggagaaatt    10140

tgcggcccgg attgcaccga ttaaaaccga tattgaaacc ctttcaaaag gcgttcaggg    10200

atggtgtgaa gcgaaccgcg acgaactgac gaacggcggc aaagtgaaga cggcgaatct    10260

tgtcaccggt gatgtatcgt ggcgggtccg tccaccatca gtaagtattc gtggtatgga    10320

tgcagtgatg gaaacgctgg agcgtcttgg cctgcaacgc tttattcgca cgaagcagga    10380

aatcaacaag gaagcgattt tactggaacc gaaagcggtc gcaggcgttg ccggaattac    10440

agttaaatca ggcattgagg atttttctat tattccattt gaacaggaag ccggtattta    10500

attggctgtt ttggcggatg agagaagatt ttcagcggaa acacagaaaa aagcccgcac    10560

ctgacagtgc gggctttttt tttcgaccaa aggtccatgg gtatggacag ttttcccttt    10620

gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata    10680

gatacaagag ccataagaac c                                              10701


<210>  72
<211>  10593
<212>  DNA
<213>  Artificial

<220>
<223>  pCas9-LambdaGam


<220>
<221>  misc_feature
<222>  (9946)..(9976)
<223>  n is a, c, g, or t

<400>  72
tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag       60

ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc      120

aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt      180

acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg      240

gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa      300

cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa      360

atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt      420

ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt      480

tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc      540

aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg      600

caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt      660

ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat      720

cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat      780

ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat      840

cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc      900

atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa      960

ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact     1020

agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact     1080

tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta     1140

tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga     1200

tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc     1260

gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac     1320

cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg     1380

accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct     1440

ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa     1500

ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg     1560

ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc     1620

tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc     1680

agcggtatca tcaacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     1740

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     1800

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttacgttt ccacaaccaa     1860

ttaaccaatt ctgatttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat     1920

atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc     1980

accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc     2040

aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc     2100

accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac     2160

ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt     2220

attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt     2280

acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc     2340

acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt     2400

gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa     2460

ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt     2520

gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc     2580

acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt     2640

ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct     2700

tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg     2760

tgcaatgtaa catcagagat tttgagacac aacgtggctt tccctgcagg atttcggagg     2820

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2880

gctcgccgca gccgaacgcc ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt     2940

ttcagagggt attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta     3000

aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg     3060

atcaccggaa aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgtg     3120

gagggactag tggattttac ggctagctca gtcctaggta caatgctagc gaattcatta     3180

aagaggagaa aggtacccat ggcacgtacc ccgagccgta gcagcattgg tagcctgcgt     3240

agtccgcata cccataaagc aattctgacc agcaccattg aaatcctgaa agaatgtggt     3300

tatagcggtc tgagcattga aagcgttgca cgtcgtgccg gtgcaagcaa accgaccatt     3360

tatcgttggt ggaccaataa agcagcactg attgccgaag tgtatgaaaa tgaaagcgaa     3420

caggtgcgta aatttccgga tctgggtagc tttaaagccg atctggattt tctgctgcgt     3480

aatctgtgga aagtttggcg tgaaaccatt tgtggtgaag catttcgttg tgttattgca     3540

gaagcacagc tggaccctgc aaccctgacc cagctgaaag atcagtttat ggaacgtcgt     3600

cgtgagatgc cgaaaaaact ggttgaaaat gccattagca atggtgaact gccgaaagat     3660

accaatcgtg aactgctgct ggatatgatt tttggttttt gttggtatcg cctgctgacc     3720

gaacagctga ccgttgaaca ggatattgaa gaatttacct tcctgctgat taatggtgtt     3780

tgtccgggta cacagcgtta actagggccc atacccccaa ttattgaagg ccgctaacgc     3840

ggcctttttt tgtttctggt ctgcccgacg tacggtgaat ctgattcgtt accaattgac     3900

atgatacgaa acgtaccgta tcgttaaggt tactagagat taaagaggag aaatactaga     3960

tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga     4020

tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc     4080

acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag     4140

cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt     4200

atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac     4260

ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa     4320

atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa     4380

aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata     4440

tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg     4500

tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta     4560

ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac     4620

gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc     4680

tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag     4740

atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc     4800

aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt     4860

tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa     4920

tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac     4980

aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag     5040

gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag     5100

aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca     5160

agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg     5220

ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg     5280

aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc     5340

gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag     5400

ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa     5460

atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt     5520

ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt     5580

caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg     5640

ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt     5700

caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta     5760

ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt     5820

taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc     5880

acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac     5940

gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag     6000

attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata     6060

gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac     6120

atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg     6180

taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta     6240

ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta     6300

tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg     6360

ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag     6420

acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca     6480

ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg     6540

ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa     6600

actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa     6660

cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat     6720

tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata     6780

ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta     6840

aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt     6900

accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat     6960

atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa     7020

tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta     7080

atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc     7140

ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg     7200

ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac     7260

agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg     7320

ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt     7380

attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta     7440

aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact     7500

ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat     7560

atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac     7620

aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc     7680

attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc     7740

agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta     7800

ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac     7860

caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc     7920

ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag     7980

aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg     8040

atttgagtca gctaggaggt gactaactcg agtaaggatc tccaggcatt gcaggcatgc     8100

ctcgagatgc atggcgccta acctaaactg atgacgcatc ctcacgataa tatccgggta     8160

ggcgcaatca ctttcgtcta ctccgttaca aagcgaggct gggtatttcc cggcctttct     8220

gttatccgaa atccactgaa agcacagcgg ctggctgagg agataaataa taaacgaggg     8280

gctgtatgca caaagcatct tctgttgagt taagaacgag tatcgagatg gcacatagcc     8340

ttgctcaaat tggaatcagg tttgtgccaa taccagtaga aacagacgaa gaatccatgg     8400

gtatggacag atctcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga     8460

ctagccttat tttaacttgc tatttctagc tctaaaacgg gttttcccag tcacgacgtg     8520

ctagcattat acctaggact gagctagctg tcagccattc gatggtgtca acgtaaatgc     8580

atgccgcttc gcctcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat     8640

gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc     8700

aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact     8760

ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc     8820

cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc     8880

cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag     8940

acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca     9000

tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga     9060

caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg     9120

cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc     9180

ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca     9240

tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag     9300

taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga     9360

ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat     9420

tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct     9480

ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt     9540

aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct     9600

tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc     9660

agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg     9720

cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa     9780

agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc     9840

tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa     9900

ctctctactg tttctccata cccgtttttt tgggctagcg aattcnnnnn nnnnnnnnnn     9960

nnnnnnnnnn nnnnnnatgg atattaatac tgaaactgag atcaagcaaa agcattcact    10020

aacccccttt cctgttttcc taatcagccc ggcatttcgc gggcgatatt ttcacagcta    10080

tttcaggagt tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg    10140

ggcgcgtcac taccagcagc tcgcccgtga agagaaagag gcagaactgg cagacgacat    10200

ggaaaaaggc ctgccccagc acctgtttga atcgctatgc atcgatcatt tgcaacgcca    10260

cggggccagc aaaaaatcca ttacccgtgc gtttgatgac gatgttgagt ttcaggagcg    10320

catggcagaa cacatccggt acatggttga aaccattgct caccaccagg ttgatattga    10380

ttcagaggta taattggctg ttttggcgga tgagagaaga ttttcagcgg aaacacagaa    10440

aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggtccat gggtatggac    10500

agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat    10560

gcttcactga tagatacaag agccataaga acc                                 10593


