                         SEQUENCE LISTING

<110>  H. Lundbeck A/S
 
<120>  New Monoclonal Anti-Alpha-Synuclein Antibodies

<130>  0992

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR 1 Heavy Chain

<400>  1

Gly Phe Thr Phe Ser Ser Tyr Ala Met Thr 
1               5                   10  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR2 Heavy Chain

<400>  2

Ala Ile Arg Ser Asn Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR3 Heavy Chain

<400>  3

Ala Lys Asn Trp Ala Pro Phe Asp Ser 
1               5                   


<210>  4
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR1 Light Chain

<400>  4

Ala Ser Gln Ser Val Ser Ser Ser Tyr Leu Ala 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR 2 Light Chain

<400>  5

Gly Ala Ser Ser Arg Ala Thr 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR 3 Light Chain

<400>  6

Gln Gln Tyr Gly Ser Ser Pro Trp Thr 
1               5                   


<210>  7
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 CDR Heavy Chain

<400>  7

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Arg Ser Asn Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Trp Ala Pro Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  8
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  GM 37 Light Chain

<400>  8

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  9
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Epitope 112-117

<400>  9

Ile Leu Glu Asp Met Pro 
1               5       


<210>  10
<211>  140
<212>  PRT
<213>  Artificial

<220>
<223>  Alpha-synuclein

<400>  10

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


<210>  11
<211>  165
<212>  PRT
<213>  Artificial

<220>
<223>  A-Syn-AAKK-BAP

<400>  11

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Asn Ile Ala Ala Ala Thr Gly Leu Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Ala Lys Gln Asn Glu Glu Gly Phe Leu Gln Glu Gly 
            100                 105                 110         


Met Val Asn Asn Thr Asp Ile Pro Val Asp Pro Glu Asn Glu Ala Tyr 
        115                 120                 125             


Glu Met Pro Pro Glu Glu Glu Tyr Gln Asp Tyr Glu Pro Glu Ala Gly 
    130                 135                 140                 


Ser Ala Gly Gly Ser Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
145                 150                 155                 160 


Ile Glu Trp His Glu 
                165 


<210>  12
<211>  165
<212>  PRT
<213>  Artificial

<220>
<223>  A-Syn-BAAK-BAP

<400>  12

Met Asp Val Phe Met Lys Gly Leu Ser Met Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Thr Glu Ala Ala Glu Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Ala Lys Gln Asn Glu Glu Gly Phe Leu Gln Glu Gly 
            100                 105                 110         


Met Val Asn Asn Thr Asp Ile Pro Val Asp Pro Glu Asn Glu Ala Tyr 
        115                 120                 125             


Glu Met Pro Pro Glu Glu Glu Tyr Gln Asp Tyr Glu Pro Glu Ala Gly 
    130                 135                 140                 


Ser Ala Gly Gly Ser Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
145                 150                 155                 160 


Ile Glu Trp His Glu 
                165 


<210>  13
<211>  162
<212>  PRT
<213>  Artificial

<220>
<223>  A-Syn-BBAA-BAP

<400>  13

Met Asp Val Phe Met Lys Gly Leu Ser Met Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Thr Glu Ala Ala Glu Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Arg Glu Gly Val 
        35                  40                  45              


Val Gln Gly Val Ala Ser Val Ala Glu Lys Thr Lys Glu Gln Ala Ser 
    50                  55                  60                  


His Leu Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala Gly Ser Ala Gly 
    130                 135                 140                 


Gly Ser Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp 
145                 150                 155                 160 


His Glu 
        


<210>  14
<211>  165
<212>  PRT
<213>  Artificial

<220>
<223>  A-Syn-BBKK-BAP

<400>  14

Met Asp Val Phe Met Lys Gly Leu Ser Met Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Thr Glu Ala Ala Glu Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Arg Glu Gly Val 
        35                  40                  45              


Val Gln Gly Val Ala Ser Val Ala Glu Lys Thr Lys Glu Gln Ala Ser 
    50                  55                  60                  


His Leu Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Asn Ile Ala Ala Ala Thr Gly Leu Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Ala Lys Gln Asn Glu Glu Gly Phe Leu Gln Glu Gly 
            100                 105                 110         


Met Val Asn Asn Thr Asp Ile Pro Val Asp Pro Glu Asn Glu Ala Tyr 
        115                 120                 125             


Glu Met Pro Pro Glu Glu Glu Tyr Gln Asp Tyr Glu Pro Glu Ala Gly 
    130                 135                 140                 


Ser Ala Gly Gly Ser Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
145                 150                 155                 160 


Ile Glu Trp His Glu 
                165 


<210>  15
<211>  141
<212>  PRT
<213>  Artificial

<220>
<223>  A-Syn-120-140_Del-BAP

<400>  15

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Gly Ser Ala Gly Gly Ser Gly Gly Leu 
        115                 120                 125             


Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
    130                 135                 140     


<210>  16
<211>  131
<212>  PRT
<213>  Artificial

<220>
<223>  alpha-synuclein amino acids 1-119

<400>  16

Met Ala His His His His His His Ile Glu Gly Arg Met Asp Val Phe 
1               5                   10                  15      


Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val Ala Ala Ala Glu 
            20                  25                  30          


Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys Thr Lys Glu Gly 
        35                  40                  45              


Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val Val His Gly Val 
    50                  55                  60                  


Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr Asn Val Gly Gly 
65                  70                  75                  80  


Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys Thr Val Glu Gly 
                85                  90                  95      


Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys Lys Asp Gln Leu 
            100                 105                 110         


Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile Leu Glu Asp Met 
        115                 120                 125             


Pro Val Asp 
    130     


<210>  17
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  kappa (LC constant region)

<400>  17

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  18
<211>  329
<212>  PRT
<213>  Artificial

<220>
<223>  IgG1 (HC Constant region)

<400>  18

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  19
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 epitope 112-115

<400>  19

Ile Leu Glu Asp 
1               


<210>  20
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR1 Heavy Chain

<400>  20

Ala Ala Ser Gly Phe Thr Phe Ser Arg Phe Thr Met Thr 
1               5                   10              


<210>  21
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR2 Heavy Chain

<400>  21

Ala Ile Ser Gly Ser Gly Gly Gly Thr Ser Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  22
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR3 Heavy Chain

<400>  22

Ala Lys Asn Trp Ala Pro Phe Asp Tyr 
1               5                   


<210>  23
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR1 Light Chain

<400>  23

Arg Ala Ser Gln Ser Val Ser Arg Ser Tyr Leu Ala 
1               5                   10          


<210>  24
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR2 Light Chain

<400>  24

Gly Ala Ser Ser Arg Ala Thr 
1               5           


<210>  25
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 CDR3 Light Chain

<400>  25

Gln Gln Tyr Gly Ser Ser Pro Trp Thr 
1               5                   


<210>  26
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 VH

<400>  26

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Phe 
            20                  25                  30          


Thr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Gly Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Leu Thr Val Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Trp Ala Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  27
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 VL

<400>  27

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Arg Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Val Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  28
<211>  329
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 IgG1 constant region

<400>  28

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  29
<211>  106
<212>  PRT
<213>  Artificial

<220>
<223>  GM285 Kappa chain

<400>  29

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  30
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 Variant 1 heavy chain

<400>  30

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Arg Ser Ser Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Trp Ala Pro Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  31
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  GM 37 variant 2 heavy chain

<400>  31

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Arg Ser Gln Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Trp Ala Pro Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  32
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  GM 37 variant 3 heavy chain

<400>  32

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Arg Ser His Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Trp Ala Pro Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  33
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 variant 1 heavy chain CDR 2

<400>  33

Ala Ile Arg Ser Ser Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  34
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 variant 2 CDR 2 heavy chain

<400>  34

Ala Ile Arg Ser Gln Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  35
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  GM37 variant 3 CDR 2 heavy chain

<400>  35

Ala Ile Arg Ser His Gly Asp Arg Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  36
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  9E4 binding epitope

<400>  36

Asn Glu Ala Tyr Glu 
1               5   


<210>  37
<211>  134
<212>  PRT
<213>  Artificial

<220>
<223>  HUMAN Beta-synuclein

<400>  37

Met Asp Val Phe Met Lys Gly Leu Ser Met Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Thr Glu Ala Ala Glu Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Arg Glu Gly Val 
        35                  40                  45              


Val Gln Gly Val Ala Ser Val Ala Glu Lys Thr Lys Glu Gln Ala Ser 
    50                  55                  60                  


His Leu Gly Gly Ala Val Phe Ser Gly Ala Gly Asn Ile Ala Ala Ala 
65                  70                  75                  80  


Thr Gly Leu Val Lys Arg Glu Glu Phe Pro Thr Asp Leu Lys Pro Glu 
                85                  90                  95      


Glu Val Ala Gln Glu Ala Ala Glu Glu Pro Leu Ile Glu Pro Leu Met 
            100                 105                 110         


Glu Pro Glu Gly Glu Ser Tyr Glu Asp Pro Pro Gln Glu Glu Tyr Gln 
        115                 120                 125             


Glu Tyr Glu Pro Glu Ala 
    130                 


<210>  38
<211>  127
<212>  PRT
<213>  Artificial

<220>
<223>  HUMAN Gamma-synuclein

<400>  38

Met Asp Val Phe Lys Lys Gly Phe Ser Ile Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Gly Ala Val Glu Lys Thr Lys Gln Gly Val Thr Glu Ala Ala Glu Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Met Tyr Val Gly Ala Lys Thr Lys Glu Asn Val 
        35                  40                  45              


Val Gln Ser Val Thr Ser Val Ala Glu Lys Thr Lys Glu Gln Ala Asn 
    50                  55                  60                  


Ala Val Ser Glu Ala Val Val Ser Ser Val Asn Thr Val Ala Thr Lys 
65                  70                  75                  80  


Thr Val Glu Glu Ala Glu Asn Ile Ala Val Thr Ser Gly Val Val Arg 
                85                  90                  95      


Lys Glu Asp Leu Arg Pro Ser Ala Pro Gln Gln Glu Gly Glu Ala Ser 
            100                 105                 110         


Lys Glu Lys Glu Glu Val Ala Glu Glu Ala Gln Ser Gly Gly Asp 
        115                 120                 125         


<210>  39
<211>  140
<212>  PRT
<213>  Artificial

<220>
<223>  alpha-synuclein ortholog for Cynomolgus monkey

<400>  39

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Ile Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Gln Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


<210>  40
<211>  140
<212>  PRT
<213>  Artificial

<220>
<223>  alpha-synuclein ortholog for Rat

<400>  40

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Thr Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Asn Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Met Gly Lys Gly Glu Glu Gly Tyr Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Ser Ser Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


<210>  41
<211>  140
<212>  PRT
<213>  Artificial

<220>
<223>  alpha-synuclein ortholog for Mouse

<400>  41

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Thr Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Asn Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Met Gly Lys Gly Glu Glu Gly Tyr Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Gly Ser Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


<210>  42
<211>  446
<212>  PRT
<213>  artificial

<220>
<223>  9E4 HC

<400>  42

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Asn Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Ala Gly Ile Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  43
<211>  220
<212>  PRT
<213>  artificial

<220>
<223>  9E4 LC

<400>  43

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ile Gln Thr Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Ala Pro Lys Leu Leu Ile Tyr Trp Ala Ser Ile Arg Lys Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Leu Ala Thr Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
        115                 120                 125             


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
145                 150                 155                 160 


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
            180                 185                 190         


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
        195                 200                 205             


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 220 


