Sequence listing:
<110>  Evonik Degussa GmbH

<120>  Method for producing methionine

<130>  1

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Primer 1 for Corynebacterium humireducens chromosome

<400>  1
gccatatgca caaccagacc aagct                                            25


<210>  2
<211>  29
<212>  DNA
<213>  Primer 2 for Corynebacterium humireducens chromosome

<400>  2
agcggccgct taggcctggg acttctcca                                        29


<210>  3
<211>  30
<212>  DNA
<213>  Primer 3 for Corynebacterium humireducens chromosome

<400>  3
agcggccgct taggacttct ccagggcctg                                       30


<210>  4
<211>  48
<212>  DNA
<213>  Primer 4 for Corynebacterium humireducens chromosome

<400>  4
agcggccgct taggcctggg acttctcgat ggcctgctcg atgtcggc                   48


<210>  5
<211>  25
<212>  DNA
<213>  Primer 1 for Corynebacterium glutamicum chromosome

<400>  5
gccatatgcc aaagtacgac aattc                                            25


<210>  6
<211>  29
<212>  DNA
<213>  Primer 2 for Corynebacterium glutamicum chromosome

<400>  6
agcggccgcc tagattgcag caaagccgc                                        29


<210>  7
<211>  1272
<212>  DNA
<213>  metY Corynebacterium humireducens


<220>
<221>  CDS
<222>  (1)..(1272)

<400>  7
atg cac aac cag acc aag ctc atc cac tcc ggc tat gtc ccg ggc aac        48
Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn           
1               5                   10                  15                

aag gac ccc cga cag gtg ccg atc gtg cag tcc acg acc tac acc ttc        96
Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe           
            20                  25                  30                    

gac tcc tcc gag gac atc gcg gcg gtg ttc gat gag ccg acc cac gcg       144
Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala           
        35                  40                  45                        

ctg atc tac tcc cgc ttc gcc aac ccg acc gtc atg gcg gtg gag acg       192
Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr           
    50                  55                  60                            

aag atc gcc gag ctg gag ggc ggt gcc gcg gcg atg gcg acc tcc tcc       240
Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser           
65                  70                  75                  80            

ggc cag gcc gcg acc gcg ctg tcg atc atg aac gtc tgc tcg gcg ggt       288
Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly           
                85                  90                  95                

gac tcc ttc gtg gcc tcc tcc gag atc tac ggc ggc acc ctc aac ctc       336
Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu           
            100                 105                 110                   

ttc cac acg acg ctc aag cgt ttc ggc atc gag gtc atc tac gtc gac       384
Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp           
        115                 120                 125                       

cag gac gcc tcc gag gag gag atc gcc gcc gcc ttc aag ccg aac acg       432
Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr           
    130                 135                 140                           

aag gca ctg ttc ggt gag acc atc ggc aac ccg gcg atg agc gtg ctg       480
Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu           
145                 150                 155                 160           

gac atc gag aag ttc gcc cgc atc gcc cac ggt cag ggc gtg ccg ctc       528
Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu           
                165                 170                 175               

atc gtc gac tcc acc ttc gcc acc ccg atg ctg tgc cgc ccc atc gag       576
Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu           
            180                 185                 190                   

cac ggc gcc gac gtg gtc atc cac tcc acc tcc aag tac atg gac ggc       624
His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly           
        195                 200                 205                       

cac gcg ctg cag gtc ggc ggc atg atc gtc gac gcc ggc acc ttc gac       672
His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp           
    210                 215                 220                           

tac acc aac ggc aag ttc ccg ggc ttc acc gag ccg gac gag tcc tac       720
Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr           
225                 230                 235                 240           

cac ggc gtc gtg tac acc aag gac tac gcc gtt gcc ccc ttc atc atc       768
His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile           
                245                 250                 255               

aag gcc cgc atg cag ctg cag cgt gat ttc ggc gcc tac ccg tcc gcg       816
Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala           
            260                 265                 270                   

cac tcc gcc ttc atg ctc aac cac tcc ctc gag agc ctc gac gtg cgc       864
His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg           
        275                 280                 285                       

atg cag cgc cac tgc gag aac gca cag agg gtc gcc gag tac ctc gag       912
Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu           
    290                 295                 300                           

ggc cgc aag gac gtg ctt gtc gac gtc cgc tac ccc gga ctc gac tcc       960
Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser           
305                 310                 315                 320           

tcc ccg tac cgc gag ctg gcg aag aag tac ctc agc ggc aac tcc ggc      1008
Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly           
                325                 330                 335               

gtg ctg tgc atc gac atc aag ggg ggc cgc gag gcc ggc gtg aag ttc      1056
Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe           
            340                 345                 350                   

atg gac gcc ctc gag ctg gtc acc cgc cag gtg cac gtc gcc gac gcc      1104
Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala           
        355                 360                 365                       

cgt tcc tgc gtg ctg cac ccg gcg tcg acc acg cac cgc cag gtg ccg      1152
Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro           
    370                 375                 380                           

gac gag cag ctg ccg tcc gtc ggc atc acc ccg ggt ctg gtg cgc atc      1200
Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile           
385                 390                 395                 400           

tcc gtc ggc ctg gag aac gtc gac gac atc atc gcc gac atc gag cag      1248
Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln           
                405                 410                 415               

gcc ctg gag aag tcc cag gcc taa                                      1272
Ala Leu Glu Lys Ser Gln Ala                                               
            420                                                           


<210>  8
<211>  423
<212>  PRT
<213>  MetY Corynebacterium humireducens

<400>  8

Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn 
1               5                   10                  15      


Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe 
            20                  25                  30          


Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala 
        35                  40                  45              


Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr 
    50                  55                  60                  


Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser 
65                  70                  75                  80  


Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly 
                85                  90                  95      


Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu 
            100                 105                 110         


Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp 
        115                 120                 125             


Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr 
    130                 135                 140                 


Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu 
145                 150                 155                 160 


Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu 
                165                 170                 175     


Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu 
            180                 185                 190         


His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly 
        195                 200                 205             


His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp 
    210                 215                 220                 


Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr 
225                 230                 235                 240 


His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile 
                245                 250                 255     


Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala 
            260                 265                 270         


His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg 
        275                 280                 285             


Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu 
    290                 295                 300                 


Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser 
305                 310                 315                 320 


Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly 
                325                 330                 335     


Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe 
            340                 345                 350         


Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala 
        355                 360                 365             


Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro 
    370                 375                 380                 


Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile 
385                 390                 395                 400 


Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln 
                405                 410                 415     


Ala Leu Glu Lys Ser Gln Ala 
            420             


<210>  9
<211>  1266
<212>  DNA
<213>  metY_421Stop Corynebacterium humireducens


<220>
<221>  CDS
<222>  (1)..(1266)

<400>  9
atg cac aac cag acc aag ctc atc cac tcc ggc tat gtc ccg ggc aac        48
Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn           
1               5                   10                  15                

aag gac ccc cga cag gtg ccg atc gtg cag tcc acg acc tac acc ttc        96
Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe           
            20                  25                  30                    

gac tcc tcc gag gac atc gcg gcg gtg ttc gat gag ccg acc cac gcg       144
Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala           
        35                  40                  45                        

ctg atc tac tcc cgc ttc gcc aac ccg acc gtc atg gcg gtg gag acg       192
Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr           
    50                  55                  60                            

aag atc gcc gag ctg gag ggc ggt gcc gcg gcg atg gcg acc tcc tcc       240
Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser           
65                  70                  75                  80            

ggc cag gcc gcg acc gcg ctg tcg atc atg aac gtc tgc tcg gcg ggt       288
Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly           
                85                  90                  95                

gac tcc ttc gtg gcc tcc tcc gag atc tac ggc ggc acc ctc aac ctc       336
Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu           
            100                 105                 110                   

ttc cac acg acg ctc aag cgt ttc ggc atc gag gtc atc tac gtc gac       384
Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp           
        115                 120                 125                       

cag gac gcc tcc gag gag gag atc gcc gcc gcc ttc aag ccg aac acg       432
Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr           
    130                 135                 140                           

aag gca ctg ttc ggt gag acc atc ggc aac ccg gcg atg agc gtg ctg       480
Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu           
145                 150                 155                 160           

gac atc gag aag ttc gcc cgc atc gcc cac ggt cag ggc gtg ccg ctc       528
Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu           
                165                 170                 175               

atc gtc gac tcc acc ttc gcc acc ccg atg ctg tgc cgc ccc atc gag       576
Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu           
            180                 185                 190                   

cac ggc gcc gac gtg gtc atc cac tcc acc tcc aag tac atg gac ggc       624
His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly           
        195                 200                 205                       

cac gcg ctg cag gtc ggc ggc atg atc gtc gac gcc ggc acc ttc gac       672
His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp           
    210                 215                 220                           

tac acc aac ggc aag ttc ccg ggc ttc acc gag ccg gac gag tcc tac       720
Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr           
225                 230                 235                 240           

cac ggc gtc gtg tac acc aag gac tac gcc gtt gcc ccc ttc atc atc       768
His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile           
                245                 250                 255               

aag gcc cgc atg cag ctg cag cgt gat ttc ggc gcc tac ccg tcc gcg       816
Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala           
            260                 265                 270                   

cac tcc gcc ttc atg ctc aac cac tcc ctc gag agc ctc gac gtg cgc       864
His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg           
        275                 280                 285                       

atg cag cgc cac tgc gag aac gca cag agg gtc gcc gag tac ctc gag       912
Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu           
    290                 295                 300                           

ggc cgc aag gac gtg ctt gtc gac gtc cgc tac ccc gga ctc gac tcc       960
Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser           
305                 310                 315                 320           

tcc ccg tac cgc gag ctg gcg aag aag tac ctc agc ggc aac tcc ggc      1008
Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly           
                325                 330                 335               

gtg ctg tgc atc gac atc aag ggg ggc cgc gag gcc ggc gtg aag ttc      1056
Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe           
            340                 345                 350                   

atg gac gcc ctc gag ctg gtc acc cgc cag gtg cac gtc gcc gac gcc      1104
Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala           
        355                 360                 365                       

cgt tcc tgc gtg ctg cac ccg gcg tcg acc acg cac cgc cag gtg ccg      1152
Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro           
    370                 375                 380                           

gac gag cag ctg ccg tcc gtc ggc atc acc ccg ggt ctg gtg cgc atc      1200
Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile           
385                 390                 395                 400           

tcc gtc ggc ctg gag aac gtc gac gac atc atc gcc gac atc gag cag      1248
Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln           
                405                 410                 415               

gcc ctg gag aag tcc taa                                              1266
Ala Leu Glu Lys Ser                                                       
            420                                                           


<210>  10
<211>  421
<212>  PRT
<213>  MetY_421Stop Corynebacterium humireducens

<400>  10

Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn 
1               5                   10                  15      


Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe 
            20                  25                  30          


Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala 
        35                  40                  45              


Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr 
    50                  55                  60                  


Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser 
65                  70                  75                  80  


Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly 
                85                  90                  95      


Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu 
            100                 105                 110         


Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp 
        115                 120                 125             


Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr 
    130                 135                 140                 


Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu 
145                 150                 155                 160 


Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu 
                165                 170                 175     


Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu 
            180                 185                 190         


His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly 
        195                 200                 205             


His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp 
    210                 215                 220                 


Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr 
225                 230                 235                 240 


His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile 
                245                 250                 255     


Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala 
            260                 265                 270         


His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg 
        275                 280                 285             


Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu 
    290                 295                 300                 


Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser 
305                 310                 315                 320 


Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly 
                325                 330                 335     


Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe 
            340                 345                 350         


Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala 
        355                 360                 365             


Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro 
    370                 375                 380                 


Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile 
385                 390                 395                 400 


Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln 
                405                 410                 415     


Ala Leu Glu Lys Ser 
            420     


<210>  11
<211>  1272
<212>  DNA
<213>  metY_L418I Corynebacterium humireducens


<220>
<221>  CDS
<222>  (1)..(1272)

<400>  11
atg cac aac cag acc aag ctc atc cac tcc ggc tat gtc ccg ggc aac        48
Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn           
1               5                   10                  15                

aag gac ccc cga cag gtg ccg atc gtg cag tcc acg acc tac acc ttc        96
Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe           
            20                  25                  30                    

gac tcc tcc gag gac atc gcg gcg gtg ttc gat gag ccg acc cac gcg       144
Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala           
        35                  40                  45                        

ctg atc tac tcc cgc ttc gcc aac ccg acc gtc atg gcg gtg gag acg       192
Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr           
    50                  55                  60                            

aag atc gcc gag ctg gag ggc ggt gcc gcg gcg atg gcg acc tcc tcc       240
Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser           
65                  70                  75                  80            

ggc cag gcc gcg acc gcg ctg tcg atc atg aac gtc tgc tcg gcg ggt       288
Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly           
                85                  90                  95                

gac tcc ttc gtg gcc tcc tcc gag atc tac ggc ggc acc ctc aac ctc       336
Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu           
            100                 105                 110                   

ttc cac acg acg ctc aag cgt ttc ggc atc gag gtc atc tac gtc gac       384
Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp           
        115                 120                 125                       

cag gac gcc tcc gag gag gag atc gcc gcc gcc ttc aag ccg aac acg       432
Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr           
    130                 135                 140                           

aag gca ctg ttc ggt gag acc atc ggc aac ccg gcg atg agc gtg ctg       480
Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu           
145                 150                 155                 160           

gac atc gag aag ttc gcc cgc atc gcc cac ggt cag ggc gtg ccg ctc       528
Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu           
                165                 170                 175               

atc gtc gac tcc acc ttc gcc acc ccg atg ctg tgc cgc ccc atc gag       576
Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu           
            180                 185                 190                   

cac ggc gcc gac gtg gtc atc cac tcc acc tcc aag tac atg gac ggc       624
His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly           
        195                 200                 205                       

cac gcg ctg cag gtc ggc ggc atg atc gtc gac gcc ggc acc ttc gac       672
His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp           
    210                 215                 220                           

tac acc aac ggc aag ttc ccg ggc ttc acc gag ccg gac gag tcc tac       720
Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr           
225                 230                 235                 240           

cac ggc gtc gtg tac acc aag gac tac gcc gtt gcc ccc ttc atc atc       768
His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile           
                245                 250                 255               

aag gcc cgc atg cag ctg cag cgt gat ttc ggc gcc tac ccg tcc gcg       816
Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala           
            260                 265                 270                   

cac tcc gcc ttc atg ctc aac cac tcc ctc gag agc ctc gac gtg cgc       864
His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg           
        275                 280                 285                       

atg cag cgc cac tgc gag aac gca cag agg gtc gcc gag tac ctc gag       912
Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu           
    290                 295                 300                           

ggc cgc aag gac gtg ctt gtc gac gtc cgc tac ccc gga ctc gac tcc       960
Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser           
305                 310                 315                 320           

tcc ccg tac cgc gag ctg gcg aag aag tac ctc agc ggc aac tcc ggc      1008
Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly           
                325                 330                 335               

gtg ctg tgc atc gac atc aag ggg ggc cgc gag gcc ggc gtg aag ttc      1056
Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe           
            340                 345                 350                   

atg gac gcc ctc gag ctg gtc acc cgc cag gtg cac gtc gcc gac gcc      1104
Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala           
        355                 360                 365                       

cgt tcc tgc gtg ctg cac ccg gcg tcg acc acg cac cgc cag gtg ccg      1152
Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro           
    370                 375                 380                           

gac gag cag ctg ccg tcc gtc ggc atc acc ccg ggt ctg gtg cgc atc      1200
Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile           
385                 390                 395                 400           

tcc gtc ggc ctg gag aac gtc gac gac atc atc gcc gac atc gag cag      1248
Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln           
                405                 410                 415               

gcc atc gag aag tcc cag gcc taa                                      1272
Ala Ile Glu Lys Ser Gln Ala                                               
            420                                                           


<210>  12
<211>  423
<212>  PRT
<213>  MetY_L418I Corynebacterium humireducens

<400>  12

Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn 
1               5                   10                  15      


Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe 
            20                  25                  30          


Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala 
        35                  40                  45              


Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr 
    50                  55                  60                  


Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser 
65                  70                  75                  80  


Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly 
                85                  90                  95      


Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu 
            100                 105                 110         


Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp 
        115                 120                 125             


Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr 
    130                 135                 140                 


Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu 
145                 150                 155                 160 


Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu 
                165                 170                 175     


Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu 
            180                 185                 190         


His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly 
        195                 200                 205             


His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp 
    210                 215                 220                 


Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr 
225                 230                 235                 240 


His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile 
                245                 250                 255     


Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala 
            260                 265                 270         


His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg 
        275                 280                 285             


Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu 
    290                 295                 300                 


Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser 
305                 310                 315                 320 


Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly 
                325                 330                 335     


Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe 
            340                 345                 350         


Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala 
        355                 360                 365             


Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro 
    370                 375                 380                 


Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile 
385                 390                 395                 400 


Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln 
                405                 410                 415     


Ala Ile Glu Lys Ser Gln Ala 
            420             


<210>  13
<211>  1314
<212>  DNA
<213>  metY Corynebacterium glutamicum


<220>
<221>  CDS
<222>  (1)..(1314)

<400>  13
atg cca aag tac gac aat tcc aat gct gac cag tgg ggc ttt gaa acc        48
Met Pro Lys Tyr Asp Asn Ser Asn Ala Asp Gln Trp Gly Phe Glu Thr           
1               5                   10                  15                

cgc tcc att cac gca ggc cag tca gta gac gca cag acc agc gca cga        96
Arg Ser Ile His Ala Gly Gln Ser Val Asp Ala Gln Thr Ser Ala Arg           
            20                  25                  30                    

aac ctt ccg atc tac caa tcc acc gct ttc gtg ttc gac tcc gct gag       144
Asn Leu Pro Ile Tyr Gln Ser Thr Ala Phe Val Phe Asp Ser Ala Glu           
        35                  40                  45                        

cac gcc aag cag cgt ttc gca ctt gag gat cta ggc cct gtt tac tcc       192
His Ala Lys Gln Arg Phe Ala Leu Glu Asp Leu Gly Pro Val Tyr Ser           
    50                  55                  60                            

cgc ctc acc aac cca acc gtt gag gct ttg gaa aac cgc atc gct tcc       240
Arg Leu Thr Asn Pro Thr Val Glu Ala Leu Glu Asn Arg Ile Ala Ser           
65                  70                  75                  80            

ctc gaa ggt ggc gtc cac gct gta gcg ttc tcc tcc gga cag gcc gca       288
Leu Glu Gly Gly Val His Ala Val Ala Phe Ser Ser Gly Gln Ala Ala           
                85                  90                  95                

acc acc aac gcc att ttg aac ctg gca gga gcg ggc gac cac atc gtc       336
Thr Thr Asn Ala Ile Leu Asn Leu Ala Gly Ala Gly Asp His Ile Val           
            100                 105                 110                   

acc tcc cca cgc ctc tac ggt ggc acc gag act cta ttc ctt atc act       384
Thr Ser Pro Arg Leu Tyr Gly Gly Thr Glu Thr Leu Phe Leu Ile Thr           
        115                 120                 125                       

ctt aac cgc ctg ggt atc gat gtt tcc ttc gtg gaa aac ccc gac gac       432
Leu Asn Arg Leu Gly Ile Asp Val Ser Phe Val Glu Asn Pro Asp Asp           
    130                 135                 140                           

cct gag tcc tgg cag gca gcc gtt cag cca aac acc aaa gca ttc ttc       480
Pro Glu Ser Trp Gln Ala Ala Val Gln Pro Asn Thr Lys Ala Phe Phe           
145                 150                 155                 160           

ggc gag act ttc gcc aac cca cag gca gac gtc ctg gat att cct gcg       528
Gly Glu Thr Phe Ala Asn Pro Gln Ala Asp Val Leu Asp Ile Pro Ala           
                165                 170                 175               

gtg gct gaa gtt gcg cac cgc aac agc gtt cca ctg atc atc gac aac       576
Val Ala Glu Val Ala His Arg Asn Ser Val Pro Leu Ile Ile Asp Asn           
            180                 185                 190                   

acc atc gct acc gca gcg ctc gtg cgc ccg ctc gag ctc ggc gca gac       624
Thr Ile Ala Thr Ala Ala Leu Val Arg Pro Leu Glu Leu Gly Ala Asp           
        195                 200                 205                       

gtt gtc gtc gct tcc ctc acc aag ttc tac acc ggc aac ggc tcc gga       672
Val Val Val Ala Ser Leu Thr Lys Phe Tyr Thr Gly Asn Gly Ser Gly           
    210                 215                 220                           

ctg ggc ggc gtg ctt atc gac ggc gga aag ttc gat tgg act gtc gaa       720
Leu Gly Gly Val Leu Ile Asp Gly Gly Lys Phe Asp Trp Thr Val Glu           
225                 230                 235                 240           

aag gat gga aag cca gta ttc ccc tac ttc gtc act cca gat gct gct       768
Lys Asp Gly Lys Pro Val Phe Pro Tyr Phe Val Thr Pro Asp Ala Ala           
                245                 250                 255               

tac cac gga ttg aag tac gca gac ctt ggt gca cca gcc ttc ggc ctc       816
Tyr His Gly Leu Lys Tyr Ala Asp Leu Gly Ala Pro Ala Phe Gly Leu           
            260                 265                 270                   

aag gtt cgc gtt ggc ctt cta cgc gac acc ggc tcc acc ctc tcc gca       864
Lys Val Arg Val Gly Leu Leu Arg Asp Thr Gly Ser Thr Leu Ser Ala           
        275                 280                 285                       

ttc aac gca tgg gct gca gtc cag ggc atc gac acc ctt tcc ctg cgc       912
Phe Asn Ala Trp Ala Ala Val Gln Gly Ile Asp Thr Leu Ser Leu Arg           
    290                 295                 300                           

ctg gag cgc cac aac gaa aac gcc atc aag gtt gca gaa ttc ctc aac       960
Leu Glu Arg His Asn Glu Asn Ala Ile Lys Val Ala Glu Phe Leu Asn           
305                 310                 315                 320           

aac cac gag aag gtg gaa aag gtt aac ttc gca ggc ctg aag gat tcc      1008
Asn His Glu Lys Val Glu Lys Val Asn Phe Ala Gly Leu Lys Asp Ser           
                325                 330                 335               

cct tgg tac gca acc aag gaa aag ctt ggc ctg aag tac acc ggc tcc      1056
Pro Trp Tyr Ala Thr Lys Glu Lys Leu Gly Leu Lys Tyr Thr Gly Ser           
            340                 345                 350                   

gtt ctc acc ttc gag atc aag ggc ggc aag gat gag gct tgg gca ttt      1104
Val Leu Thr Phe Glu Ile Lys Gly Gly Lys Asp Glu Ala Trp Ala Phe           
        355                 360                 365                       

atc gac gcc ctg aag cta cac tcc aac ctt gca aac atc ggc gat gtt      1152
Ile Asp Ala Leu Lys Leu His Ser Asn Leu Ala Asn Ile Gly Asp Val           
    370                 375                 380                           

cgc tcc ctc gtt gtt cac cca gca acc acc acc cat tca cag tcc gac      1200
Arg Ser Leu Val Val His Pro Ala Thr Thr Thr His Ser Gln Ser Asp           
385                 390                 395                 400           

gaa gct ggc ctg gca cgc gcg ggc gtt acc cag tcc acc gtc cgc ctg      1248
Glu Ala Gly Leu Ala Arg Ala Gly Val Thr Gln Ser Thr Val Arg Leu           
                405                 410                 415               

tcc gtt ggc atc gag acc att gat gat atc atc gct gac ctc gaa ggc      1296
Ser Val Gly Ile Glu Thr Ile Asp Asp Ile Ile Ala Asp Leu Glu Gly           
            420                 425                 430                   

ggc ttt gct gca atc tag                                              1314
Gly Phe Ala Ala Ile                                                       
        435                                                               


<210>  14
<211>  437
<212>  PRT
<213>  MetY Corynebacterium glutamicum

<400>  14

Met Pro Lys Tyr Asp Asn Ser Asn Ala Asp Gln Trp Gly Phe Glu Thr 
1               5                   10                  15      


Arg Ser Ile His Ala Gly Gln Ser Val Asp Ala Gln Thr Ser Ala Arg 
            20                  25                  30          


Asn Leu Pro Ile Tyr Gln Ser Thr Ala Phe Val Phe Asp Ser Ala Glu 
        35                  40                  45              


His Ala Lys Gln Arg Phe Ala Leu Glu Asp Leu Gly Pro Val Tyr Ser 
    50                  55                  60                  


Arg Leu Thr Asn Pro Thr Val Glu Ala Leu Glu Asn Arg Ile Ala Ser 
65                  70                  75                  80  


Leu Glu Gly Gly Val His Ala Val Ala Phe Ser Ser Gly Gln Ala Ala 
                85                  90                  95      


Thr Thr Asn Ala Ile Leu Asn Leu Ala Gly Ala Gly Asp His Ile Val 
            100                 105                 110         


Thr Ser Pro Arg Leu Tyr Gly Gly Thr Glu Thr Leu Phe Leu Ile Thr 
        115                 120                 125             


Leu Asn Arg Leu Gly Ile Asp Val Ser Phe Val Glu Asn Pro Asp Asp 
    130                 135                 140                 


Pro Glu Ser Trp Gln Ala Ala Val Gln Pro Asn Thr Lys Ala Phe Phe 
145                 150                 155                 160 


Gly Glu Thr Phe Ala Asn Pro Gln Ala Asp Val Leu Asp Ile Pro Ala 
                165                 170                 175     


Val Ala Glu Val Ala His Arg Asn Ser Val Pro Leu Ile Ile Asp Asn 
            180                 185                 190         


Thr Ile Ala Thr Ala Ala Leu Val Arg Pro Leu Glu Leu Gly Ala Asp 
        195                 200                 205             


Val Val Val Ala Ser Leu Thr Lys Phe Tyr Thr Gly Asn Gly Ser Gly 
    210                 215                 220                 


Leu Gly Gly Val Leu Ile Asp Gly Gly Lys Phe Asp Trp Thr Val Glu 
225                 230                 235                 240 


Lys Asp Gly Lys Pro Val Phe Pro Tyr Phe Val Thr Pro Asp Ala Ala 
                245                 250                 255     


Tyr His Gly Leu Lys Tyr Ala Asp Leu Gly Ala Pro Ala Phe Gly Leu 
            260                 265                 270         


Lys Val Arg Val Gly Leu Leu Arg Asp Thr Gly Ser Thr Leu Ser Ala 
        275                 280                 285             


Phe Asn Ala Trp Ala Ala Val Gln Gly Ile Asp Thr Leu Ser Leu Arg 
    290                 295                 300                 


Leu Glu Arg His Asn Glu Asn Ala Ile Lys Val Ala Glu Phe Leu Asn 
305                 310                 315                 320 


Asn His Glu Lys Val Glu Lys Val Asn Phe Ala Gly Leu Lys Asp Ser 
                325                 330                 335     


Pro Trp Tyr Ala Thr Lys Glu Lys Leu Gly Leu Lys Tyr Thr Gly Ser 
            340                 345                 350         


Val Leu Thr Phe Glu Ile Lys Gly Gly Lys Asp Glu Ala Trp Ala Phe 
        355                 360                 365             


Ile Asp Ala Leu Lys Leu His Ser Asn Leu Ala Asn Ile Gly Asp Val 
    370                 375                 380                 


Arg Ser Leu Val Val His Pro Ala Thr Thr Thr His Ser Gln Ser Asp 
385                 390                 395                 400 


Glu Ala Gly Leu Ala Arg Ala Gly Val Thr Gln Ser Thr Val Arg Leu 
                405                 410                 415     


Ser Val Gly Ile Glu Thr Ile Asp Asp Ile Ile Ala Asp Leu Glu Gly 
            420                 425                 430         


Gly Phe Ala Ala Ile 
        435         


<210>  15
<211>  1215
<212>  DNA
<213>  metZ_I3N_F65Y_V104A Rhodobacter sphaeroides


<220>
<221>  CDS
<222>  (1)..(1215)

<400>  15
atg ggt aac gcg ttt cgt gaa gga cgg acg ggc atg acg aag gac tgg        48
Met Gly Asn Ala Phe Arg Glu Gly Arg Thr Gly Met Thr Lys Asp Trp           
1               5                   10                  15                

aag aca agg acg caa ctc gtc cac ggg ggc agc cgc cgg agc cag tat        96
Lys Thr Arg Thr Gln Leu Val His Gly Gly Ser Arg Arg Ser Gln Tyr           
            20                  25                  30                    

ggc gaa atg gcc gag gcg atc ttc ctg acc cag ggc ttc gtc tac gac       144
Gly Glu Met Ala Glu Ala Ile Phe Leu Thr Gln Gly Phe Val Tyr Asp           
        35                  40                  45                        

tcg gcc gca cag gcc gaa gcg cgc ttc atc gag acc ggc gcc gac gaa       192
Ser Ala Ala Gln Ala Glu Ala Arg Phe Ile Glu Thr Gly Ala Asp Glu           
    50                  55                  60                            

tac atc tat gcc cgc tac ggc aac ccc acg acg cgc atg ttc gaa gag       240
Tyr Ile Tyr Ala Arg Tyr Gly Asn Pro Thr Thr Arg Met Phe Glu Glu           
65                  70                  75                  80            

cgc atc gcg gcc gtc gag ggc acc gag gat gcg ttc gcc acc gcc tcg       288
Arg Ile Ala Ala Val Glu Gly Thr Glu Asp Ala Phe Ala Thr Ala Ser           
                85                  90                  95                

ggc atg gcc gcg atc cac ggc gcg ctc acc tcg atc gtg cgg gcg ggc       336
Gly Met Ala Ala Ile His Gly Ala Leu Thr Ser Ile Val Arg Ala Gly           
            100                 105                 110                   

gat cat ctg gtg gcg gcg cgc gct ctg ttc ggc tcc tgc atc tac atc       384
Asp His Leu Val Ala Ala Arg Ala Leu Phe Gly Ser Cys Ile Tyr Ile           
        115                 120                 125                       

ctc gag gag gtg ctg ggc cga ttc ggc gtc gag gtg acc ttc gtc gac       432
Leu Glu Glu Val Leu Gly Arg Phe Gly Val Glu Val Thr Phe Val Asp           
    130                 135                 140                           

ggc acc gat ctc gat cag tgg cgc gcg gcg gtg cgg ccc ggc acg aag       480
Gly Thr Asp Leu Asp Gln Trp Arg Ala Ala Val Arg Pro Gly Thr Lys           
145                 150                 155                 160           

gcc gtg ttc ttc gag tcg gtc tcg aat ccg acg ctc gag gtg gcc gat       528
Ala Val Phe Phe Glu Ser Val Ser Asn Pro Thr Leu Glu Val Ala Asp           
                165                 170                 175               

atc ggc gcc atc gcc gag atc gcc cat gcc gtg ggc gcg ctc gtc atc       576
Ile Gly Ala Ile Ala Glu Ile Ala His Ala Val Gly Ala Leu Val Ile           
            180                 185                 190                   

gtg gac aat gtc ttc gcg acg ccc gtc ttc tcg acg gcg gtg cgg cag       624
Val Asp Asn Val Phe Ala Thr Pro Val Phe Ser Thr Ala Val Arg Gln           
        195                 200                 205                       

ggc gcg gat gtg gtg atc tat tcg gcc acc aag cac atc gac ggg caa       672
Gly Ala Asp Val Val Ile Tyr Ser Ala Thr Lys His Ile Asp Gly Gln           
    210                 215                 220                           

ggg cgc gcg ctc ggc ggc gtg gtc tgc gcc tcg cag gcc ttc atc cgc       720
Gly Arg Ala Leu Gly Gly Val Val Cys Ala Ser Gln Ala Phe Ile Arg           
225                 230                 235                 240           

aag gtg ctc gaa ccc ttc atg aag cac acc ggc ggc tcg atg agc ccc       768
Lys Val Leu Glu Pro Phe Met Lys His Thr Gly Gly Ser Met Ser Pro           
                245                 250                 255               

ttc aac gcc tgg ctc atg ctg aac ggg atg gcg acg ctc gac ctg cgc       816
Phe Asn Ala Trp Leu Met Leu Asn Gly Met Ala Thr Leu Asp Leu Arg           
            260                 265                 270                   

tgc cgc gcg atg gcc gac acg gcc gag aag atc gcc cgc gcg ctc gag       864
Cys Arg Ala Met Ala Asp Thr Ala Glu Lys Ile Ala Arg Ala Leu Glu           
        275                 280                 285                       

ggc cat ccg cag ctc ggc cgc gtg atc cat ccc gcg ctg gaa agc cac       912
Gly His Pro Gln Leu Gly Arg Val Ile His Pro Ala Leu Glu Ser His           
    290                 295                 300                           

ccg cag cac gag atg gcc aag gcg cag atg gag cgt ccc ggc acg atg       960
Pro Gln His Glu Met Ala Lys Ala Gln Met Glu Arg Pro Gly Thr Met           
305                 310                 315                 320           

atc gcg ctc gac ctc gcc ggg ggc aag gag gcg gcc ttc cgc ttc ctc      1008
Ile Ala Leu Asp Leu Ala Gly Gly Lys Glu Ala Ala Phe Arg Phe Leu           
                325                 330                 335               

gac gcc ctg agg atc gtg aag atc tcc aac aat ctg ggc gat gcc cgc      1056
Asp Ala Leu Arg Ile Val Lys Ile Ser Asn Asn Leu Gly Asp Ala Arg           
            340                 345                 350                   

tcg atc gcg acc cac ccg gca acg acc acc cac cag cgt ctt tcc gac      1104
Ser Ile Ala Thr His Pro Ala Thr Thr Thr His Gln Arg Leu Ser Asp           
        355                 360                 365                       

gcg cag aag gcc cat ctc ggc atc acg ccc ggg ctc gtg cgg ctg tcg      1152
Ala Gln Lys Ala His Leu Gly Ile Thr Pro Gly Leu Val Arg Leu Ser           
    370                 375                 380                           

gtg ggg ctc gag gat gcg gac gac ctg atc gcc gat ctg aaa cag gcg      1200
Val Gly Leu Glu Asp Ala Asp Asp Leu Ile Ala Asp Leu Lys Gln Ala           
385                 390                 395                 400           

ctc gcg gtg atc tga                                                  1215
Leu Ala Val Ile                                                           
                                                                          


<210>  16
<211>  404
<212>  PRT
<213>  MetZ_I3N_F65Y_V104A Rhodobacter sphaeroides

<400>  16

Met Gly Asn Ala Phe Arg Glu Gly Arg Thr Gly Met Thr Lys Asp Trp 
1               5                   10                  15      


Lys Thr Arg Thr Gln Leu Val His Gly Gly Ser Arg Arg Ser Gln Tyr 
            20                  25                  30          


Gly Glu Met Ala Glu Ala Ile Phe Leu Thr Gln Gly Phe Val Tyr Asp 
        35                  40                  45              


Ser Ala Ala Gln Ala Glu Ala Arg Phe Ile Glu Thr Gly Ala Asp Glu 
    50                  55                  60                  


Tyr Ile Tyr Ala Arg Tyr Gly Asn Pro Thr Thr Arg Met Phe Glu Glu 
65                  70                  75                  80  


Arg Ile Ala Ala Val Glu Gly Thr Glu Asp Ala Phe Ala Thr Ala Ser 
                85                  90                  95      


Gly Met Ala Ala Ile His Gly Ala Leu Thr Ser Ile Val Arg Ala Gly 
            100                 105                 110         


Asp His Leu Val Ala Ala Arg Ala Leu Phe Gly Ser Cys Ile Tyr Ile 
        115                 120                 125             


Leu Glu Glu Val Leu Gly Arg Phe Gly Val Glu Val Thr Phe Val Asp 
    130                 135                 140                 


Gly Thr Asp Leu Asp Gln Trp Arg Ala Ala Val Arg Pro Gly Thr Lys 
145                 150                 155                 160 


Ala Val Phe Phe Glu Ser Val Ser Asn Pro Thr Leu Glu Val Ala Asp 
                165                 170                 175     


Ile Gly Ala Ile Ala Glu Ile Ala His Ala Val Gly Ala Leu Val Ile 
            180                 185                 190         


Val Asp Asn Val Phe Ala Thr Pro Val Phe Ser Thr Ala Val Arg Gln 
        195                 200                 205             


Gly Ala Asp Val Val Ile Tyr Ser Ala Thr Lys His Ile Asp Gly Gln 
    210                 215                 220                 


Gly Arg Ala Leu Gly Gly Val Val Cys Ala Ser Gln Ala Phe Ile Arg 
225                 230                 235                 240 


Lys Val Leu Glu Pro Phe Met Lys His Thr Gly Gly Ser Met Ser Pro 
                245                 250                 255     


Phe Asn Ala Trp Leu Met Leu Asn Gly Met Ala Thr Leu Asp Leu Arg 
            260                 265                 270         


Cys Arg Ala Met Ala Asp Thr Ala Glu Lys Ile Ala Arg Ala Leu Glu 
        275                 280                 285             


Gly His Pro Gln Leu Gly Arg Val Ile His Pro Ala Leu Glu Ser His 
    290                 295                 300                 


Pro Gln His Glu Met Ala Lys Ala Gln Met Glu Arg Pro Gly Thr Met 
305                 310                 315                 320 


Ile Ala Leu Asp Leu Ala Gly Gly Lys Glu Ala Ala Phe Arg Phe Leu 
                325                 330                 335     


Asp Ala Leu Arg Ile Val Lys Ile Ser Asn Asn Leu Gly Asp Ala Arg 
            340                 345                 350         


Ser Ile Ala Thr His Pro Ala Thr Thr Thr His Gln Arg Leu Ser Asp 
        355                 360                 365             


Ala Gln Lys Ala His Leu Gly Ile Thr Pro Gly Leu Val Arg Leu Ser 
    370                 375                 380                 


Val Gly Leu Glu Asp Ala Asp Asp Leu Ile Ala Asp Leu Lys Gln Ala 
385                 390                 395                 400 


Leu Ala Val Ile     



201500159 A	2




