                         SEQUENCE LISTING

<110>  University of Copenhagen
       Novo Nordisk A/S
       Henrik, Clausen
       Yang, Zhang
       Halim, Adnan Fevzi
       Bennett, Eric
       Behrens, Carsten
       Vester-Christensen, Malene Bech
       Rahman, Shahim Herbert
 
<120>  Production of N-glycoproteins for enzyme assisted 
       glycomodification

<130>  56275PC01

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat1-WT

<400>  1
aacaagttca agttcccagc agctgtggta gtggaggac                              39


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat1-alle1

<400>  2
aacaagttca agttccgcag ctgtggtagt ggaggac                                37


<210>  3
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat2-WT

<400>  3
tcctttgtcg cccattgctg ctccagagga cgaagccgca ggcggccacc acga             54


<210>  4
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat2-alle1

<400>  4
tcctttgtcg cccattgctg ctccacgaag ccgcaggcgg ccaccacga                   49


<210>  5
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat3-WT

<400>  5
ttcctggacc acttcccacc cggtggccgg caggatggc                              39


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat3-alle1

<400>  6
ttcctggacc actatggc                                                     18


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat3-alle2 (N-terminus)

<400>  7
ttcctggacc actgataccc                                                   20


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat3-alle2 (C-terminus)

<400>  8
cggtggccgg caggatggc                                                    19


<210>  9
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4A-WT

<400>  9
ttctgagttg aatgccattg tccaacagtt tcgccgtgca ggagcagcaa ctaacggaag       60


<210>  10
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4A-alle1

<400>  10
ttctgagttg aatgccattg tccaacagcc gtgcaggagc agcaactaac ggaag            55


<210>  11
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4A-alle2

<400>  11
ttctgagttg aatgccattg tccaacagtt gaattctaga tgatcgccgt gcaggagcag       60

caactaacgg aag                                                          73


<210>  12
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4B-WT

<400>  12
gccctccagc agccctctga ggactggatg atcctggagt t                           41


<210>  13
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4B-alle1

<400>  13
gccctccagc agccctctgg atgatcctgg agtt                                   34


<210>  14
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4C-WT

<400>  14
atacttcaga ctattatgta atgctcgaag atgatgtt                               38


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4C-alle1

<400>  15
atacttcaga ctcgaagatg atgtt                                             25


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat4C-alle2

<400>  16
atacttcaga ctatgctcga agatgatgtt                                        30


<210>  17
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-WT

<400>  17
gggggatgat gcttctgcac ttcaccatcc agcagcggac tcagcctgag agcagctcca       60

tgtt                                                                    64


<210>  18
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle1

<400>  18
gggggatgat gcttctgcac ttcaccatcc agcatgaatt ctagatgagc ggactcagcc       60

tgagagcagc tccatgtt                                                     78


<210>  19
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle2

<400>  19
gggggatgat gcttctgcac ttcaccatcc agagcggact cagcctgaga gcagctccat       60

gtt                                                                     63


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle3 (N-terminus)

<400>  20
gggggatgat gcttctgcac ttcaccatcc agcag                                  35


<210>  21
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle3 (C-terminus)

<400>  21
cggactcagc ctgagagcag ctccatgtt                                         29


<210>  22
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5B-WT

<400>  22
cgtggcgccc tccgcaagat gagtgacctg ctggagctg                              39


<210>  23
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5B-alle1

<400>  23
cagctccagc aggtcttgcg gagggcgcca cg                                     32


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5B-alle2

<400>  24
cagctccagc aggtatcttg cggagggcgc cacg                                   34


<210>  25
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt1-WT

<400>  25
tgcagctgct ctacctgtcg ctgctctccg gactgcacg                              39


<210>  26
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt1-alle1

<400>  26
tgcagctgct ctacctgtcg cagctgctct acctgtctcc ggactgcacg                  50


<210>  27
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt1-alle2

<400>  27
tgcagctgct ctacctgtct ctccggactg cacg                                   34


<210>  28
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt2-WT

<400>  28
ttcagccctt cccgggcgta ctggaacaga gagca                                  35


<210>  29
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt2-alle1

<400>  29
ttcagccctt ccgggcgtac tggaacagag agca                                   34


<210>  30
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt2-alle2

<400>  30
ttcagccctt cccgtactgg aacagagagc a                                      31


<210>  31
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt1-WT

<400>  31
tgcatccggt cctacagcgc cagcaactgg actatggta                              39


<210>  32
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt1-alle1

<400>  32
tgcatccggt cctacagcgc cagccagcaa ctggactatg gta                         43


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt2-WT

<400>  33
cagccccgcc actttgccat cgccatggac aagtttggct                             40


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt2-alle1 (N-terminus)

<400>  34
cagccccgcc actttgcc                                                     18


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt2-alle1 (C-terminus)

<400>  35
atcgccatgg acaagtttgg ct                                                22


<210>  36
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt3-WT

<400>  36
ctagccctca agtcaggatg ttgcggaggc tgctggagag g                           41


<210>  37
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt3-alle1

<400>  37
ctagccctca agtcaggatg tcgtgttgcg gaggctgctg gagagg                      46


<210>  38
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt3-alle2

<400>  38
ctagccctca agtcaggatg ttgcccggag gctgctggag agg                         43


<210>  39
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt4-WT

<400>  39
aactgggact gctttatatt ccacgatgtg gacctggtg                              39


<210>  40
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt4-alle1

<400>  40
aactgggact gctttattat tccacgatgt ggacctggtg                             40


<210>  41
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt4-alle2

<400>  41
aactgggact gctttatatt tattccacga tgtggacctg gtg                         43


<210>  42
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal3-WT

<400>  42
ctctctcttt gtccttgctg gcttcaaatg gcaggacttc aag                         43


<210>  43
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal3-alle1

<400>  43
ctctctcttt gtccttgctc aaatggcagg acttcaag                               38


<210>  44
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal3-alle2

<400>  44
ctctctcttt gtccttgctg gctcaaatgg caggacttca ag                          42


<210>  45
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-WT

<400>  45
ggcagcctcc agtgtcgtcg ttgtgttgtg gtggggaatg ggc                         43


<210>  46
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-alle1

<400>  46
ggcagcctcc agtgtcgtcg ttgtttgtgt tgtggtgggg aatgggc                     47


<210>  47
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-alle2

<400>  47
ggcagcctcc agtgtcgtcg gttgtggtgg ggaatgggc                              39


<210>  48
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-WT

<400>  48
cggtacctct gattttgctt tgccctatgg gacaaggcc                              39


<210>  49
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-alle1

<400>  49
cggtacctct gattttgctt ctatgggaca aggcc                                  35


<210>  50
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-alle2

<400>  50
cggtacctct gaaggcc                                                      17


<210>  51
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fut8-WT

<400>  51
caaatacttg atccgtccac aaccttggct ggaaagggaa                             40


<210>  52
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fut8-alle1

<400>  52
caaatacttg atccgtccac aacctggctg gaaagggaa                              39


<210>  53
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fut8-alle2

<400>  53
caaatacttg atccgtccac aacctttggc tggaaaggga a                           41


<210>  54
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN with 60bp homology arm sequences

<400>  54
gagagagatt ggtcttgctt gtcatcacca acgtatgaac cagtgtgatg gtgaaatgag       60

tcgccgtgca ggagcagcaa ctaacggaag taacactgca ttgactacat tttcaggtac      120


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mgataex1F

<400>  55
tatccactgt gttgcttgct g                                                 21


<210>  56
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mgataex2R

<400>  56
actgctcttc cagaggtcct g                                                 21


<210>  57
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mgataex2F

<400>  57
gaacgccttc gaatagctga acatagg                                           27


<210>  58
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mgat4aEx1gRNA

<400>  58
ggtataccac atggcaaaat ggg                                               23


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mgat4aEx2gRNA

<400>  59
gtccaacagt ttcgccgtgc agg                                               23


<210>  60
<211>  3873
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Shuttle vector

<400>  60
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accaagcttg      420

gttgcatgct gtccggagtc tcagcgttat accagaagtg acctgggtcg gggaagacta      480

tagtgtcacc taaatctcta gagcccttca ttaggcgcgc caatcccatt gcaaattcta      540

caaaaggagt gtttcccaac tgctctatca agaggaatgt tgcacactgt gacctgaatg      600

caaacatcac acgcgccagc agagaggaag aagagaggct tccctgaccg ggaatcgaac      660

ccgggccgcg gcggtgagag cgccgaatcc taaccactag accaccaggg agcacgcgcc      720

aaagctcaat gagctataat tatccccttg gaaaacctac aaaaacagtg tttcaaaact      780

gctctgtgaa aagggacctt tgctagcacg cggcgccagg caaaacgtgg gcacgctgcg      840

ttggccggga atcgaacccg ggtcaactgc ttggaaggca gctatgctca ccactatacc      900

accaacgcgc acacgcgcca gcagattcta cgggaagagt gtttcaaaac tgctctatca      960

agagaaatgt tccaccttgt gtgtggaatg cagccatcac acgcgtccat gaaagggctt     1020

aattaagata tcgtttaaac gtcgacctgc agaggccggc ggataactag ctgatcgcgg     1080

aatcctgtcc ctaggccacc cactgtgggg tgcccttcat taggcgcgcc aatcccattg     1140

caaattctac aaaaggagtg tttcccaact gctctatcaa gaggaatgtt gcacactgtg     1200

acctgaatgc aaacatcaca cgcgccagca gagaggaaga agagaggctt ccctgaccgg     1260

gaatcgaacc cgggccgcgg cggtgagagc gccgaatcct aaccactaga ccaccaggga     1320

gcacgcgcca aagctcaatg agctataatt atccccttgg aaaacctaca aaaacagtgt     1380

ttcaaaactg ctctgtgaaa agggaccttt gctagcacgc ggcgccaggc aaaacgtggg     1440

cacgctgcgt tggccgggaa tcgaacccgg gtcaactgct tggaaggcag ctatgctcac     1500

cactatacca ccaacgcgca cacgcgccag cagattctac gggaagagtg tttcaaaact     1560

gctctatcaa gagaaatgtt ccaccttgtg tgtggaatgc agccatcaca cgcgtccatg     1620

aaagggcggt tgcatgctgt ccggagtctc agcgttatac cagaagtgac ctgggtcggg     1680

gaagaaaagc ttcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     1740

cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct     1800

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc     1860

agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     1920

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     1980

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     2040

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     2100

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     2160

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     2220

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     2280

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     2340

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     2400

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     2460

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     2520

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     2580

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     2640

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     2700

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     2760

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     2820

caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca     2880

aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt     2940

tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc     3000

ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa     3060

taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa     3120

gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat     3180

cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc     3240

gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg     3300

ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg     3360

ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct     3420

tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa     3480

catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc     3540

catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc     3600

catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt     3660

gaatatggct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     3720

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     3780

catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct     3840

ataaaaatag gcgtatcacg aggccctttc gtc                                  3873


<210>  61
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat2-alle1

<400>  61
tcctttgtcg cccattgctg ctccgacgaa gccgcaggcg gccaccacga                  50


<210>  62
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle1

<400>  62
gggggatgat gcttctgcac ttcaccatcc agcagccagc ggactcagcc tgagagcagc       60

tccatgtt                                                                68


<210>  63
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle2

<400>  63
gggggatgat gcttctgcac ttcaccatcc agggactcag cctgagagca gctccatgtt       60


<210>  64
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat5-alle3

<400>  64
gggggatgat gcttctgcac ttcctcagcc tgagagcagc tccatgtt                    48


<210>  65
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt2-alle1

<400>  65
cagccccgcc accgccatgg acaagtttgg ct                                     32


<210>  66
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B4galt2-alle2

<400>  66
cagccccgcc atggacaagt ttggct                                            26


<210>  67
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-alle1

<400>  67
ggcagcctcc agtgtcgtcg gttgtggtgg ggaatgggc                              39


<210>  68
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-alle1

<400>  68
ggcagcctcc agtgtcgtcg ttgtggtggg gaatgggc                               38


<210>  69
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal4-alle2

<400>  69
ggcagcctcc agtgtcgtcg acacgttgtg ttgtggtggg gaatgggc                    48


<210>  70
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-alle1

<400>  70
cggtacctct gattttgctt ttgccctatg ggacaaggcc                             40


<210>  71
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-alle2

<400>  71
cggtacctct gattttgctt tgctatggga caaggcc                                37


<210>  72
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  st3Gal6-alle2

<400>  72
cggtacctct gattttgcta tgggacaagg cc                                     32


<210>  73
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt2-alle1

<400>  73
ttcagccctt cccccgggcg tactggaaca gagagca                                37


<210>  74
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt2-alle2

<400>  74
ttcagccctt ccccgggcgt actggaacag agagca                                 36


<210>  75
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt8-WT

<400>  75
tggtccagag atagctaatg aagcttctag ggtggagaag ctggggctgc tga              53


<210>  76
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3gnt8-alle1

<400>  76
tggtccagag gagggtggag aagctggggc tgctga                                 36


<210>  77
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fut8-alle1

<400>  77
caaatacttg atccgtccac aaggctggaa agggaa                                 36


<210>  78
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mgat2-alle1

<400>  78
tcctttgtcg cccattgctg ctccagcgaa gccgcaggcg gccaccacga                  50


