                         SEQUENCE LISTING

<110>  Ventana Medical Systems, Inc.
 
<120>  MATERIALS AND METHODS FOR PERFORMING HISTOCHEMICAL ASSAYS FOR 
       HUMAN PRO-EPIREGULIN AND AMPHIREGULIN

<130>  P32945-WO

<150>  US62/186,251
<151>  2015-06-29

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  169
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Thr Ala Gly Arg Arg Met Glu Met Leu Cys Ala Gly Arg Val Pro 
1               5                   10                  15      


Ala Leu Leu Leu Cys Leu Gly Phe His Leu Leu Gln Ala Val Leu Ser 
            20                  25                  30          


Thr Thr Val Ile Pro Ser Cys Ile Pro Gly Glu Ser Ser Asp Asn Cys 
        35                  40                  45              


Thr Ala Leu Val Gln Thr Glu Asp Asn Pro Arg Val Ala Gln Val Ser 
    50                  55                  60                  


Ile Thr Lys Cys Ser Ser Asp Met Asn Gly Tyr Cys Leu His Gly Gln 
65                  70                  75                  80  


Cys Ile Tyr Leu Val Asp Met Ser Gln Asn Tyr Cys Arg Cys Glu Val 
                85                  90                  95      


Gly Tyr Thr Gly Val Arg Cys Glu His Phe Phe Leu Thr Val His Gln 
            100                 105                 110         


Pro Leu Ser Lys Glu Tyr Val Ala Leu Thr Val Ile Leu Ile Ile Leu 
        115                 120                 125             


Phe Leu Ile Thr Val Val Gly Ser Thr Tyr Tyr Phe Cys Arg Trp Tyr 
    130                 135                 140                 


Arg Asn Arg Lys Ser Lys Glu Pro Lys Lys Glu Tyr Glu Arg Val Thr 
145                 150                 155                 160 


Ser Gly Asp Pro Glu Leu Pro Gln Val 
                165                 


<210>  2
<211>  5
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  2

Arg Tyr Gly Met Ser 
1               5   


<210>  3
<211>  16
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  3

Ser Ile Asn Arg Thr Ala Tyr Thr Tyr Tyr Ala Thr Trp Ala Lys Gly 
1               5                   10                  15      


<210>  4
<211>  15
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  4

Gly Leu Thr Tyr Gly Gly Ser Asp Tyr Asp Tyr Asp Asp Ala Leu 
1               5                   10                  15  


<210>  5
<211>  29
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  5

Gln Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser 
            20                  25                  


<210>  6
<211>  14
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  6

Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile Gly 
1               5                   10                  


<210>  7
<211>  30
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  7

Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr Val Asp Leu Arg Met Thr 
1               5                   10                  15      


Ser Leu Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg 
            20                  25                  30  


<210>  8
<211>  11
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  8

Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10      


<210>  9
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  9

Gln Ala Ser Gln Ser Val Tyr Lys Asn Lys Asn Leu Ala 
1               5                   10              


<210>  10
<211>  7
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  10

Arg Ala Ser Thr Leu Ala Ser 
1               5           


<210>  11
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  11

Gln Gly Glu Phe Ser Cys Ser Thr Phe Asp Cys Ile Leu 
1               5                   10              


<210>  12
<211>  22
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  12

Gln Val Leu Thr Gln Thr Pro Ser Ser Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Asn Cys 
            20          


<210>  13
<211>  15
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  13

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  14
<211>  32
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  14

Gly Val Ser Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Gly Val Gln Cys Ala Asp Ala Ala Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  15
<211>  10
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  15

Phe Gly Gly Gly Thr Glu Met Val Val Lys 
1               5                   10  


<210>  16
<211>  120
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  16

Gln Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Arg Tyr Gly 
            20                  25                  30          


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile Gly 
        35                  40                  45              


Ser Ile Asn Arg Thr Ala Tyr Thr Tyr Tyr Ala Thr Trp Ala Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr Val Asp Leu Arg Met Thr 
65                  70                  75                  80  


Ser Leu Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg Gly Leu 
                85                  90                  95      


Thr Tyr Gly Gly Ser Asp Tyr Asp Tyr Asp Asp Ala Leu Trp Gly Pro 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  17
<211>  112
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  17

Gln Val Leu Thr Gln Thr Pro Ser Ser Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Val Tyr Lys Asn Lys 
            20                  25                  30          


Asn Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe Lys 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Gly Val Gln 
65                  70                  75                  80  


Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Gln Gly Glu Phe Ser Cys Ser 
                85                  90                  95      


Thr Phe Asp Cys Ile Leu Phe Gly Gly Gly Thr Glu Met Val Val Lys 
            100                 105                 110         


<210>  18
<211>  5
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  18

Thr Phe Ala Met Ala 
1               5   


<210>  19
<211>  16
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  19

Phe Ile Ser Leu Ser Asp Ala Thr Tyr Tyr Ala Thr Trp Ala Lys Gly 
1               5                   10                  15      


<210>  20
<211>  14
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  20

Val Val Gly Asp Ser Ser Gly Tyr Pro Asn Thr Phe His Pro 
1               5                   10                  


<210>  21
<211>  29
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  21

Lys Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Ile Asp Leu Ser 
            20                  25                  


<210>  22
<211>  14
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  22

Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile Gly 
1               5                   10                  


<210>  23
<211>  31
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  23

Arg Phe Thr Ile Ser Lys Ser Ser Ser Thr Thr Val Asp Leu Lys Ile 
1               5                   10                  15      


Ile Thr Pro Thr Ala Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg 
            20                  25                  30      


<210>  24
<211>  11
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  24

Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10      


<210>  25
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  25

Gln Ala Ser Gln Ser Ile His Asn Ser Asp Phe Leu Ala 
1               5                   10              


<210>  26
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  26

Gln Ala Ser Gln Asn Ile His Asn Ser Asp Phe Leu Ala 
1               5                   10              


<210>  27
<211>  7
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  27

Arg Ala Ser Lys Leu Pro Ser 
1               5           


<210>  28
<211>  12
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  28

Gln Gly Thr Tyr Tyr Ser Gly Gly Trp Tyr Phe Thr 
1               5                   10          


<210>  29
<211>  22
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  29

Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Asn Cys 
            20          


<210>  30
<211>  15
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  30

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  31
<211>  32
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  31

Gly Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  32
<211>  10
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  32

Phe Gly Gly Gly Thr Glu Val Val Val Lys 
1               5                   10  


<210>  33
<211>  120
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  33

Lys Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Ile Asp Leu Ser Thr Phe Ala 
            20                  25                  30          


Met Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile Gly 
        35                  40                  45              


Phe Ile Ser Leu Ser Asp Ala Thr Tyr Tyr Ala Thr Trp Ala Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Lys Ser Ser Ser Thr Thr Val Asp Leu Lys Ile 
65                  70                  75                  80  


Ile Thr Pro Thr Ala Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg Val 
                85                  90                  95      


Val Gly Asp Ser Ser Gly Tyr Pro Asn Thr Phe His Pro Trp Gly Pro 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  34
<211>  111
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  34

Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Ile His Asn Ser Asp 
            20                  25                  30          


Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Ala Ser Lys Leu Pro Ser Gly Val Pro Ser Arg Phe Lys 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu Glu 
65                  70                  75                  80  


Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Gln Gly Thr Tyr Tyr Ser Gly 
                85                  90                  95      


Gly Trp Tyr Phe Thr Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 110     


<210>  35
<211>  111
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  35

Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Asn Cys Gln Ala Ser Gln Asn Ile His Asn Ser Asp 
            20                  25                  30          


Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Ala Ser Lys Leu Pro Ser Gly Val Pro Ser Arg Phe Lys 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu Glu 
65                  70                  75                  80  


Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Gln Gly Thr Tyr Tyr Ser Gly 
                85                  90                  95      


Gly Trp Tyr Phe Thr Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 110     


<210>  36
<211>  252
<212>  PRT
<213>  Homo sapiens

<400>  36

Met Arg Ala Pro Leu Leu Pro Pro Ala Pro Val Val Leu Ser Leu Leu 
1               5                   10                  15      


Ile Leu Gly Ser Gly His Tyr Ala Ala Gly Leu Asp Leu Asn Asp Thr 
            20                  25                  30          


Tyr Ser Gly Lys Arg Glu Pro Phe Ser Gly Asp His Ser Ala Asp Gly 
        35                  40                  45              


Phe Glu Val Thr Ser Arg Ser Glu Met Ser Ser Gly Ser Glu Ile Ser 
    50                  55                  60                  


Pro Val Ser Glu Met Pro Ser Ser Ser Glu Pro Ser Ser Gly Ala Asp 
65                  70                  75                  80  


Tyr Asp Tyr Ser Glu Glu Tyr Asp Asn Glu Pro Gln Ile Pro Gly Tyr 
                85                  90                  95      


Ile Val Asp Asp Ser Val Arg Val Glu Gln Val Val Lys Pro Pro Gln 
            100                 105                 110         


Asn Lys Thr Glu Ser Glu Asn Thr Ser Asp Lys Pro Lys Arg Lys Lys 
        115                 120                 125             


Lys Gly Gly Lys Asn Gly Lys Asn Arg Arg Asn Arg Lys Lys Lys Asn 
    130                 135                 140                 


Pro Cys Asn Ala Glu Phe Gln Asn Phe Cys Ile His Gly Glu Cys Lys 
145                 150                 155                 160 


Tyr Ile Glu His Leu Glu Ala Val Thr Cys Lys Cys Gln Gln Glu Tyr 
                165                 170                 175     


Phe Gly Glu Arg Cys Gly Glu Lys Ser Met Lys Thr His Ser Met Ile 
            180                 185                 190         


Asp Ser Ser Leu Ser Lys Ile Ala Leu Ala Ala Ile Ala Ala Phe Met 
        195                 200                 205             


Ser Ala Val Ile Leu Thr Ala Val Ala Val Ile Thr Val Gln Leu Arg 
    210                 215                 220                 


Arg Gln Tyr Val Arg Lys Tyr Glu Gly Glu Ala Glu Glu Arg Lys Lys 
225                 230                 235                 240 


Leu Arg Gln Glu Asn Gly Asn Val His Ala Ile Ala 
                245                 250         


<210>  37
<211>  5
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  37

Ser Tyr Ala Ile Ser 
1               5   


<210>  38
<211>  17
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  38

Phe Ile Val Gly Ser Ser Gly Ser Ala Tyr Tyr Ala Ser Trp Ala Lys 
1               5                   10                  15      


Ser 
    


<210>  39
<211>  8
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  39

Gly Leu Tyr Ser Gly Gly Asn Tyr 
1               5               


<210>  40
<211>  29
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  40

Gln Ser Leu Glu Glu Ser Arg Gly Gly Leu Ile Lys Pro Gly Gly Thr 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser 
            20                  25                  


<210>  41
<211>  14
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  41

Trp Val Arg Gln Ala Pro Gly Asn Gly Leu Glu Trp Ile Gly 
1               5                   10                  


<210>  42
<211>  32
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  42

Arg Ser Thr Ile Thr Arg Asp Thr Asn Leu Asn Thr Val Thr Leu Lys 
1               5                   10                  15      


Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys Ala Lys 
            20                  25                  30          


<210>  43
<211>  11
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  43

Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10      


<210>  44
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  44

Gln Ser Ser Gln Ser Val Asp Glu Asn Asn Tyr Leu Ser 
1               5                   10              


<210>  45
<211>  7
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  45

Arg Ala Ser Thr Leu Glu Ser 
1               5           


<210>  46
<211>  11
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  46

Leu Gly Gly Tyr Ser Gly Tyr Ser Asp Asp Gly 
1               5                   10      


<210>  47
<211>  22
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  47

Ala Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Ser Ile Ser Cys 
            20          


<210>  48
<211>  15
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  48

Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  49
<211>  32
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  49

Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Phe Thr 
1               5                   10                  15      


Leu Thr Val Ser Gly Val Gln Cys Asp Asp Ala Ala Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  50
<211>  10
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  50

Phe Gly Gly Gly Thr Glu Val Val Val Lys 
1               5                   10  


<210>  51
<211>  116
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  51

Gln Ser Leu Glu Glu Ser Arg Gly Gly Leu Ile Lys Pro Gly Gly Thr 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Ser Tyr Ala 
            20                  25                  30          


Ile Ser Trp Val Arg Gln Ala Pro Gly Asn Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Phe Ile Val Gly Ser Ser Gly Ser Ala Tyr Tyr Ala Ser Trp Ala Lys 
    50                  55                  60                  


Ser Arg Ser Thr Ile Thr Arg Asp Thr Asn Leu Asn Thr Val Thr Leu 
65                  70                  75                  80  


Lys Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys Ala 
                85                  90                  95      


Lys Gly Leu Tyr Ser Gly Gly Asn Tyr Trp Gly Pro Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  52
<211>  110
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  52

Ala Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Ser Ile Ser Cys Gln Ser Ser Gln Ser Val Asp Glu Asn Asn 
            20                  25                  30          


Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Ala Ser Thr Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Val Ser Gly Val Gln 
65                  70                  75                  80  


Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Gly Tyr Ser Gly Tyr 
                85                  90                  95      


Ser Asp Asp Gly Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 110 


