                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  Methods for RNA Promoter Identification

<130>  UCDVP116WO/2015-541-2

<140> PCT/US2016/038802
<141> 2016-06-22

<150>  US 62/185060
<151>  2015-06-26

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  misc_feature
<222>  (8)..(55)
<223>  Hammerhead ribozyme region

<220>
<221>  misc_feature
<222>  (51)..(58)
<223>  D8 complement

<220>
<221>  misc_feature
<222>  (59)..(63)
<223>  P5 complement

<220>
<221>  misc_feature
<222>  (93)..(98)
<223>  Stuffer insertion site

<220>
<221>  misc_feature
<222>  (99)..(106)
<223>  2nd D8 complement

<220>
<221>  misc_feature
<222>  (107)..(111)
<223>  2nd P5 complement

<220>
<221>  misc_feature
<222>  (126)..(172)
<223>  Hairpin catalytic core complement

<220>
<221>  misc_feature
<222>  (221)..(315)
<223>  Multiple cloning site

<220>
<221>  primer_bind
<222>  (255)..(280)
<223>  Primer 2 complement

<220>
<221>  primer_bind
<222>  (281)..(301)
<223>  RT primer 1 complement

<220>
<221>  misc_feature
<222>  (326)..(413)
<223>  HDV negative strand ribozyme

<400>  1
ataccctgtc accggatgtg ctttccggtc tgatgagtcc gtgaggacga aacaggactg       60

tcaggtggcc gaaagccacc acgtaaacta gtggatccaa caggactgtc agctagtcaa      120

ggcgtaccag gtaatatacc acaacgtgtg tttctctggt tgacttctct gtttgttgtg      180

tcattggttc ccggatctcg cattagcggc gacggggtat cctgcaggaa gcttggatcc      240

gtcgacgcgg ccgcgatcgt cggactgtag aactctgaac ccttggcacc cgagaattcc      300

agaattcggc gcgccatacc ctgtcgggtc ggcatggcat ctccacctcc tcgcggtccg      360

acctgggcat ccgaaggagg acagacgtcc actcggatgg ctaagggaga gcc             413


<210>  2

<400>  2
000

<210>  3
<211>  48
<212>  DNA
<213>  Satellite tobacco ringspot virus

<400>  3
gacagagaag tcaaccagag aaacacacgt tgtggtatat tacctggt                    48


<210>  4
<211>  50
<212>  DNA
<213>  Satellite arabis mosaic virus

<400>  4
gacagcgaag tcaaacggcg aaacacacct tgtgtggtat attacccgtt                  50


<210>  5
<211>  50
<212>  DNA
<213>  Satellite chicory yellow mottle virus

<400>  5
gacagcgaag tcagccaggg aaacacacca tgtgtggtat attatctggc                  50


<210>  6
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  6
tgaccgtcct gtc                                                          13


<210>  7
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic RNA


<220>
<221>  misc_feature
<223>  n=A, C, G or U

<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(13)
<223>  n is a, c, g, or u

<400>  7
nnnnngucnn nnn                                                          13


<210>  8
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic RNA


<220>
<221>  misc_feature
<223>  n=A, C, G, or U; m=A or C

<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (26)..(36)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  n is a, c, g, or u

<400>  8
nnnnnmgaan nnnnnnnnag aaacannnnn nnnnnnguau auuacnnnnn                  50


<210>  9
<211>  2190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  promoter
<222>  (6)..(23)
<223>  T7 RNA promoter

<220>
<221>  misc_feature
<222>  (38)..(85)
<223>  Hammerhead ribozyme region

<220>
<221>  misc_feature
<222>  (81)..(88)
<223>  D8 complement

<220>
<221>  misc_feature
<222>  (89)..(93)
<223>  P5 complement

<220>
<221>  misc_feature
<222>  (123)..(128)
<223>  Stuffer insertion site

<220>
<221>  misc_feature
<222>  (129)..(136)
<223>  2nd D8 complement

<220>
<221>  misc_feature
<222>  (137)..(141)
<223>  2nd P5 complement

<220>
<221>  misc_feature
<222>  (156)..(202)
<223>  Hairpin catalytic core complement

<220>
<221>  misc_feature
<222>  (252)..(345)
<223>  Multiple cloning site

<220>
<221>  primer_bind
<222>  (285)..(310)
<223>  Primer 2 complement

<220>
<221>  primer_bind
<222>  (311)..(330)
<223>  RT primer 1 complement

<220>
<221>  misc_feature
<222>  (357)..(442)
<223>  HDV negative strand ribozyme

<220>
<221>  rep_origin
<222>  (451)..(1065)
<223>  pMB1 origin of replication in the reverse orientation

<220>
<221>  primer_bind
<222>  (502)..(525)
<223>  Seq2 primer binding site in the reverse orientation

<220>
<221>  gene
<222>  (1225)..(2085)
<223>  beta-lactamase gene in the reverse orientation

<220>
<221>  promoter
<222>  (2086)..(2190)
<223>  beta-lactamase promoter in reverse orientation

<220>
<221>  primer_bind
<222>  (2092)..(2121)
<223>  Seq1 primer binding site

<400>  9
agatctaata gcactcacta taggggatct ataccctgtc accggatgtg ctttccggtc       60

tgatgagtcc gtgaggacga aacaggactg tcaggtggcc gaaagccacc acgtaaacta      120

gtggatccaa caggactgtc agctagtcaa ggcgtaccag gtaatatacc acaacgtgtg      180

tttctctggt tgacttctct gtttgttgtg tcattggttc ccggatctcg cattagcggc      240

gacggggtat cctgcaggaa gcttggatcc gtcgacgcgg ccgcgatcgt cggactgtag      300

aactctgaac ccttggcacc cgagaattcc agaattcggc gcgccatacc ctgtcgggtc      360

ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acagacgtcc      420

actcggatgg ctaagggaga gccatctaga cgcgttgctg gcgtttttcc ataggctccg      480

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg      540

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac      600

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca      660

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt      720

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc      780

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag      840

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac      900

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt      960

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     1020

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     1080

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     1140

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     1200

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     1260

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     1320

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     1380

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     1440

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     1500

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     1560

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     1620

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     1680

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     1740

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     1800

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     1860

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     1920

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     1980

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     2040

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     2100

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     2160

ttagaaaaat aaacaaatag gggttccgcg                                      2190


<210>  10
<211>  8313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  misc_feature
<222>  (1)..(161)
<223>  T-DNA right border

<220>
<221>  promoter
<222>  (470)..(782)
<223>  CaMV 35S promoter

<220>
<221>  misc_feature
<222>  (793)..(840)
<223>  Hammerhead ribozyme region

<220>
<221>  misc_feature
<222>  (836)..(843)
<223>  D8 complement

<220>
<221>  misc_feature
<222>  (844)..(848)
<223>  P5 complement

<220>
<221>  misc_feature
<222>  (878)..(883)
<223>  Stuffer insertion site

<220>
<221>  misc_feature
<222>  (884)..(891)
<223>  2nd D8 complement

<220>
<221>  misc_feature
<222>  (892)..(896)
<223>  2nd P5 complement

<220>
<221>  misc_feature
<222>  (911)..(957)
<223>  Hairpin catalytic core complement

<220>
<221>  misc_feature
<222>  (1007)..(1100)
<223>  Multiple cloning site

<220>
<221>  primer_bind
<222>  (1040)..(1065)

<220>
<221>  primer_bind
<222>  (1066)..(1085)

<220>
<221>  misc_feature
<222>  (1066)..(1085)
<223>  HDV negative strand ribozyme

<220>
<221>  terminator
<222>  (1200)..(1897)
<223>  CaMV 35S terminator

<220>
<221>  gene
<222>  (2235)..(3029)
<223>  neomycin phosphotransferase II gene

<220>
<221>  rep_origin
<222>  (2235)..(3029)
<223>  ColE1 origin of replication from pBR322

<220>
<221>  misc_feature
<222>  (3711)..(3857)
<223>  T-DNA left border in the reverse orientation

<220>
<221>  rep_origin
<222>  (4164)..(4779)
<223>  OriV from pRK2 in the reverse orientation

<220>
<221>  gene
<222>  (4878)..(6026)
<223>  trfA gene in the reverse orientation

<220>
<221>  gene
<222>  (6325)..(7119)
<223>  neomycin phosphotransferase III gene in the reverse orientation

<400>  10
cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa       60

atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt      120

caaacactga tagtttgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt      180

aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg      240

gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgccat tcaggctgcg      300

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      360

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      420

taaaacgacg gccagtgaat tgttaattaa gaattcgagc tccaccgcgg aaacctcctc      480

ggattccatt gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc      540

tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac      600

agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca      660

accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca      720

caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag      780

agggtatacc ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag      840

gactgtcagg tggccgaaag ccaccacgta aactagtgga tccaacagga ctgtcagcta      900

gtcaaggcgt accaggtaat ataccacaac gtgtgtttct ctggttgact tctctgtttg      960

ttgtgtcatt ggttcccgga tctcgcatta gcggcgacgg ggtatcctgc aggaagcttg     1020

gatccgtcga cgcggccgcg atcgtcggac tgtagaactc tgaacccttg gcacccgaga     1080

attccagaat tcggcgcgcc ataccctgtc gggtcggcat ggcatctcca cctcctcgcg     1140

gtccgacctg ggcatccgaa ggaggacaga cgtccactcg gatggctaag ggagagccat     1200

cgaattcgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata     1260

aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat     1320

gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat     1380

aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc taaagtccct     1440

atagatcttt gtcgtgaata taaaccagac acgagacgac taaacctgga gcccagacgc     1500

cgttcgaagc tagaagtacc gcttaggcag gaggccgtta gggaaaagat gctaaggcag     1560

ggttggttac gttgactccc ccgtaggttt ggtttaaata tgatgaagtg gacggaagga     1620

aggaggaaga caaggaagga taaggttgca ggccctgtgc aaggtaagaa gatggaaatt     1680

tgatagaggt acgctactat acttatacta tacgctaagg gaatgcttgt atttataccc     1740

tataccccct aataacccct tatcaattta agaaataatc cgcataagcc cccgcttaaa     1800

aattggtatc agagccatga ataggtctat gaccaaaact caagaggata aaacctcacc     1860

aaaatacgaa agagttctta actctaaaga taaaagatgg cgcgtggccg gcctacagta     1920

tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt     1980

ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc gggtttctgg     2040

agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa     2100

ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt     2160

cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaccggat     2220

ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg     2280

tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg     2340

tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg     2400

ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc     2460

cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg     2520

aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca     2580

tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc     2640

aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg     2700

atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg     2760

cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata     2820

tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg     2880

accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat     2940

gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct     3000

tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca     3060

agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt     3120

gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat     3180

gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg ccgatatcat     3240

tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata tgatcgcggc     3300

gtccacatca acggcgtcgg cggcgactgc ccaggcaaga ccgagatgca ccgcgatatc     3360

ttgctgcgtt cggatatttt cgtggagttc ccgccacaga cccggatgat ccccgatcgt     3420

tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt     3480

atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg     3540

ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata     3600

gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta     3660

ctagatcggg actgtaggcc ggccctcact ggtgaaaaga aaaaccaccc cagtacatta     3720

aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata     3780

tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg     3840

atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga     3900

ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac     3960

acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg     4020

tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg     4080

cttaaccgtg acagagtaga caggctgtct cgcggccgag gggcgcagcc cctggggggg     4140

atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc     4200

gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt     4260

ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa     4320

atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg     4380

tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc     4440

ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa     4500

ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc     4560

cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg     4620

tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc     4680

cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca     4740

tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg aaaggcgctc     4800

ggtcttgcct tgctcgtcgg tgatgtacac tagtcgctgg ctgctgaacc cccagccgga     4860

actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt     4920

tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc     4980

ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac     5040

ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg     5100

tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg     5160

tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc     5220

agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc     5280

tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc     5340

aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc     5400

gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg     5460

gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg     5520

cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc     5580

gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc     5640

tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg     5700

tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc     5760

gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat     5820

ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct     5880

tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg     5940

cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat     6000

gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc     6060

ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc     6120

accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta     6180

ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc     6240

tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg     6300

gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat     6360

tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc     6420

cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct     6480

gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct     6540

gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa     6600

atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac     6660

atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt     6720

cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag     6780

ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc     6840

ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg     6900

aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt     6960

ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag     7020

cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat     7080

cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt     7140

ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg     7200

ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag     7260

ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg     7320

ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct     7380

atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc     7440

ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac     7500

accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga     7560

tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa     7620

aactcaggcc gcagtcggta actatgcggt gtgaaatacc gcacagatgc gtaaggagaa     7680

aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     7740

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     7800

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     7860

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     7920

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     7980

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     8040

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     8100

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     8160

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     8220

cactggcagc aggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa     8280

atggacgggc ccccggcgcc agatctgggg aac                                  8313


<210>  11
<211>  5366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  promoter
<222>  (15)..(754)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (866)..(913)
<223>  Hammerhead ribozyme region

<220>
<221>  misc_feature
<222>  (909)..(916)
<223>  D8 complement

<220>
<221>  misc_feature
<222>  (917)..(921)
<223>  P5 complement

<220>
<221>  misc_feature
<222>  (951)..(956)
<223>  Stuffer insertion site

<220>
<221>  misc_feature
<222>  (957)..(964)
<223>  2nd D8 complement

<220>
<221>  misc_feature
<222>  (965)..(969)
<223>  2nd P5 complement

<220>
<221>  misc_feature
<222>  (984)..(1030)
<223>  Hairpin catalytic core complement

<220>
<221>  misc_feature
<222>  (1080)..(1173)
<223>  Multiple cloning site

<220>
<221>  primer_bind
<222>  (1113)..(1138)
<223>  Primer 2 complement

<220>
<221>  primer_bind
<222>  (1139)..(1158)
<223>  RT primer 1 complement

<220>
<221>  misc_feature
<222>  (1185)..(1270)
<223>  HDV negative strand ribozyme

<220>
<221>  polyA_signal
<222>  (1313)..(1532)
<223>  polyA signal sequence

<220>
<221>  promoter
<222>  (1582)..(1998)
<223>  SV40 early enhancer/promoter

<220>
<221>  gene
<222>  (2024)..(3061)
<223>  hygromycin resistance gene

<220>
<221>  rep_origin
<222>  (3274)..(3812)
<223>  ColE1 origin of replication from pBR322

<220>
<221>  gene
<222>  (4248)..(5108)
<223>  beta-lactamase gene in the reverse orientation

<220>
<221>  polyA_signal
<222>  (5213)..(5260)
<223>  polyA signal sequence

<400>  11
ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa       60

tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata      120

ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt      180

aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta      240

cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga      300

cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt      360

tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta      420

ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg      480

actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt      540

tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc      600

accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat      660

gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct      720

atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt      780

atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt      840

actgttggta aagccaccat accctgtcac cggatgtgct ttccggtctg atgagtccgt      900

gaggacgaaa caggactgtc aggtggccga aagccaccac gtaaactagt ggatccaaca      960

ggactgtcag ctagtcaagg cgtaccaggt aatataccac aacgtgtgtt tctctggttg     1020

acttctctgt ttgttgtgtc attggttccc ggatctcgca ttagcggcga cggggtatcc     1080

tgcaggaagc ttggatccgt cgacgcggcc gcgatcgtcg gactgtagaa ctctgaaccc     1140

ttggcacccg agaattccag aattcggcgc gccataccct gtcgggtcgg catggcatct     1200

ccacctcctc gcggtccgac ctgggcatcc gaaggaggac agacgtccac tcggatggct     1260

aagggagagc caggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga     1320

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta     1380

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag     1440

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt     1500

tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt     1560

gggcgctctt ccgctgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac     1620

ttggttagct accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg     1680

tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc     1740

agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca     1800

tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc     1860

gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc     1920

cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct     1980

aggcttttgc aaaaagctcg attcttctga cactagcgcc accatgaaga agcccgaact     2040

caccgctacc agcgttgaaa aatttctcat cgagaagttc gacagtgtga gcgacctgat     2100

gcagttgtcg gagggcgaag agagccgagc cttcagcttc gatgtcggcg gacgcggcta     2160

tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa gaccgctacg tgtaccgcca     2220

cttcgccagc gctgcactac ccatccccga agtgttggac atcggcgagt tcagcgagag     2280

cctgacatac tgcatcagta gacgcgccca aggcgttact ctccaagacc tccccgaaac     2340

agagctgcct gctgtgttac agcctgtcgc cgaagctatg gatgctattg ccgccgccga     2400

cctcagtcaa accagcggct tcggcccatt cgggccccaa ggcatcggcc agtacacaac     2460

ctggcgggat ttcatttgcg ccattgctga tccccatgtc taccactggc agaccgtgat     2520

ggacgacacc gtgtccgcca gcgtagctca agccctggac gaactgatgc tgtgggccga     2580

agactgtccc gaggtgcgcc acctcgtcca tgccgacttc ggcagcaaca acgtcctgac     2640

cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa gctatgttcg gggacagtca     2700

gtacgaggtg gccaacatct tcttctggcg gccctggctg gcttgcatgg agcagcagac     2760

tcgctacttc gagcgccggc atcccgagct ggccggcagc cctcgtctgc gagcctacat     2820

gctgcgcatc ggcctggatc agctctacca gagcctcgtg gacggcaact tcgacgatgc     2880

tgcctgggct caaggccgct gcgatgccat cgtccgcagc ggggccggca ccgtcggtcg     2940

cacacaaatc gctcgccgga gcgcagccgt atggaccgac ggctgcgtcg aggtgctggc     3000

cgacagcggc aaccgccggc ccagtacacg accgcgcgct aaggaggtag gtcgagttta     3060

aactctagaa ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt     3120

ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc ccttgagagc cttcaaccca     3180

gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc     3240

tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga     3300

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat     3360

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca     3420

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     3480

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     3540

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     3600

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     3660

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     3720

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     3780

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     3840

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     3900

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     3960

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     4020

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     4080

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat     4140

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga     4200

gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg     4260

atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc     4320

cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg     4380

atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg     4440

agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc     4500

tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt     4560

gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc     4620

cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta     4680

gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca     4740

gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag     4800

tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg     4860

tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat     4920

cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag     4980

cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt     5040

gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg     5100

atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc     5160

tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat     5220

atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata     5280

cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc     5340

aagtgcaggt gccagaacat ttctct                                          5366


