                         SEQUENCE LISTING

<110>  INTA
       INTA, INTA
 
<120>  Chimeric polypeptide, vaccine against babesiosis, immunization methods, detectios methods and kit

<130>  INTA

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  601
<212>  PRT
<213>  Babesia bovis

<400>  1

Ile Thr Val Ala Phe Cys Ser Ile Leu Phe Thr Tyr Thr Leu Ala Ser 
1               5                   10                  15      


Pro Gln Glu Lys Thr Val Pro Thr Lys Gln Val Asn Gly Ser His Leu 
            20                  25                  30          


Leu Ser Asp Asp Met Lys Met Phe Tyr Asp Val Met Arg Ser Ile Asp 
        35                  40                  45              


Glu Ser Met Leu Lys Ser Ile Leu Glu Lys Asn Phe Glu Ala Val Gly 
    50                  55                  60                  


Met Glu Ala Gln Ser Ala Thr Lys Thr His Asp Ala Leu Lys Ala Val 
65                  70                  75                  80  


Lys Gln Leu Ile Lys Thr Asp Ala Pro Phe Asn Thr Ser Asp Phe Asp 
                85                  90                  95      


Thr Leu Asp Leu Glu Tyr Leu Ser Gly Gln Ser Asn Glu Glu Leu Leu 
            100                 105                 110         


Lys Leu Leu Ile Glu Ala Ile Tyr Gly Met Glu Ile Ile Ile Glu Lys 
        115                 120                 125             


Thr His Ser Phe Val Gly Glu Ser Gln Lys Tyr Ser Asp Asp Val Asp 
    130                 135                 140                 


Thr Glu Leu Arg Lys Tyr Tyr Lys Ala Asn Ile Tyr Asp Asp Gln Ser 
145                 150                 155                 160 


Glu Tyr Asn Lys Asp Lys Leu Ser Asn Leu Tyr Lys Ala Phe Ile Thr 
                165                 170                 175     


Asp Ser Gly Ala Leu Arg Ile Ala Ser Glu Glu Leu Ile Lys Phe Glu 
            180                 185                 190         


Thr Arg Lys Ala Gln Lys Asn Asp Tyr Arg Phe Ile Asn Pro Ser Ser 
        195                 200                 205             


Thr Ser Glu Ala Glu Thr Pro Ser Pro Ser Ser Phe Arg His Asn Gln 
    210                 215                 220                 


Arg Val Gly Ser Leu Ala Pro Ala Glu Val Val Gly Asp Leu Thr Ser 
225                 230                 235                 240 


Thr Leu Glu Thr Ala Asp Thr Leu Met Thr Leu Arg Asp His Met His 
                245                 250                 255     


Asn Ile Thr Lys Asp Met Lys His Val Leu Ser Asn Gly Arg Glu Gln 
            260                 265                 270         


Ile Val Asn Asp Val Cys Ser Asn Ala Pro Glu Asp Ser Asn Cys Arg 
        275                 280                 285             


Glu Val Val Asn Asn Tyr Ala Asp Arg Cys Glu Met Tyr Gly Cys Phe 
    290                 295                 300                 


Thr Ile Asp Asn Val Lys Tyr Pro Leu Tyr Gln Glu Tyr Gln Pro Leu 
305                 310                 315                 320 


Ser Leu Pro Asn Pro Tyr Gln Leu Asp Ala Ala Phe Arg Leu Phe Lys 
                325                 330                 335     


Glu Ser Ala Ser Asn Pro Ala Lys Asn Ser Val Lys Arg Glu Trp Leu 
            340                 345                 350         


Arg Phe Arg Asn Gly Ala Asn His Gly Asp Tyr His Tyr Phe Val Thr 
        355                 360                 365             


Gly Leu Leu Asn Asn Asn Val Val His Gly Glu Gly Thr Thr Asp Val 
    370                 375                 380                 


Glu Tyr Leu Val Asn Lys Val Leu Tyr Met Ala Thr Met Asn Tyr Lys 
385                 390                 395                 400 


Thr Tyr Leu Thr Val Asn Ser Met Asn Ala Lys Phe Phe Asn Arg Phe 
                405                 410                 415     


Ser Phe Thr Thr Lys Ile Phe Ser Arg Arg Ile Arg Gln Thr Leu Ser 
            420                 425                 430         


Asp Ile Cys Asn Asn Ala Asp Gln Glu Val Ile Ile Asp Glu Gln Thr 
        435                 440                 445             


Gly Leu Pro Ile Lys Ser His Asp Tyr Ser Glu Lys Pro Ser Val Ile 
    450                 455                 460                 


Tyr Lys Pro Ser Thr Thr Val Pro Gln Asn Thr Leu Leu Glu Ile Pro 
465                 470                 475                 480 


Pro Pro Lys Glu Leu Glu Asn Pro Ile Thr Phe Asn Pro Thr Val Asp 
                485                 490                 495     


Thr Phe Phe Asp Ala Asp Asn Asn Lys Leu Val Leu Leu Met Glu Leu 
            500                 505                 510         


Pro Gly Phe Ser Ser Thr Asp Ile Asn Val Glu Cys Gly Trp Gly Glu 
        515                 520                 525             


Leu Ile Ile Ser Gly Pro Arg Asn Lys Asp Glu Leu Tyr Glu Lys Phe 
    530                 535                 540                 


Gly Asn Asn Leu Asp Ile His Ile Arg Glu Arg Lys Val Gly Tyr Phe 
545                 550                 555                 560 


Tyr Arg Arg Phe Lys Leu Pro Asn Asn Ala Ile Asp Lys Ser Ile Ser 
                565                 570                 575     


Val Gly Tyr Ser Asn Gly Ile Leu Asp Ile Arg Ile Glu Cys Ser Gln 
            580                 585                 590         


Phe Ser Glu Met Arg Arg Val Gln Ile 
        595                 600     


<210>  2
<211>  1818
<212>  DNA
<213>  Babesia bovis

<400>  2
atgataaccg ttgcattctg ctccatcctt ttcacttata cattggcatc tccacaagag       60

aagactgtac caactaaaca ggtgaatggg agtcatttac tgtttgatga tatgaaaatg      120

ttttatgatg taatgcgcag cattgatgaa agcatgttga aaagcattct tgagaagaat      180

tttgaggctg ttggcatgga agctacatcg gctactaaaa cacatgacgc tctgaaggct      240

gtaaaacaat taatcaaaac cgatgcacct ttcaacacat cggactttga cacactcgat      300

ttggaatatc tctcagggca atctaatgaa gagctgttga agcttctgat tgaggcaata      360

tatggtatgg aaatcataat tgaaaaaacc cactctttcg tgggagagag tcaaaaatat      420

tccgatgacg tagacaccga gttgagaaag tattacaagg ctaatattta cgatgaccag      480

agtgaatata ataaggacaa attaagcaac ctttacaagg cattcatcac tgattctggc      540

gctttgagaa tcgcatcgga ggaacttata aaattcgaaa caaggaaagc tcaaaagaat      600

gactacagat ttatcaatcc ttcttcaact tcggaagccg aaacaccctc tccatcgtct      660

ctcgagtttc gccacaatca gagagtagga agtctcgctc cagctgaagt ggtaggtgat      720

ttaacctcca cattggaaac agctgatact ttgatgactc tccgtgacca catgcacaac      780

attactaagg atatgaaaca tgttttgagc aatggccgtg agcagattgt aaatgatgtt      840

tgctctaatg ctcctgagga ctccaactgt cgtgaggtag ttaacaatta tgctgaccgt      900

tgtgaaatgt acggatgctt tacgattgac aatgtcaaat atccgttgta tcaagagtac      960

caacctctat ctcttccaaa cccttaccag ttggatgctg cgttcagatt gttcaaagag     1020

agtgcatcca atcctgctaa gaacagcgta aaacgcgaat ggttgcgttt cagaaatgga     1080

gcgaaccatg gtgattacca ctacttcgtc actggtctgt tgaacaacaa tgttgtgcac     1140

gaggaaggaa ctaccgatgt tgaatatctt gtcaacaagg tactctatat ggctaccatg     1200

aactacaaga cttatttgac agtaaacagt atgaacgcca agtttttcaa cagattcagc     1260

ttcactacaa agatattcag ccgtcgtatt aggcaaacat tgagtgatat ctgcaacaac     1320

gccgaccagg aggtcatcat cgacgagcag actggtctcc ctatcaagag ccacgactac     1380

agtgagaagc catctgtcat ctacaagcct tctaccactg tcccacagaa cactcttctt     1440

gagattcctc ctcctaagga gttggagaac ccgattactt tcaacccaac tgtggacact     1500

ttctttgatg ctgacaacaa caaattggtt ttgttgatgg aacttcctgg tttcagcagc     1560

actgacatca atgttgagtg tggttggggt gaattgatca tcagcggccc tcgtaacaag     1620

gatgagcttt acgagaaatt cggtaacaac ttggacattc acattcgtga gcgtaaggtt     1680

ggttacttct acaggcgttt caagcttccc aacagcgcca ttgacaagag cattagcgtt     1740

ggctactcta atggtatttt ggacatcagg atcgagtgct ctcaattctc tgagatgtgc     1800

cgcgttcaga ttgctagc                                                   1818


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  msa-2c   Fw

<400>  3
atggtgtctt ttaacataat aacc                                              24


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  msa-2c   Rv

<400>  4
aaatgcagag agaacgaagt ag                                                22


<210>  5
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rap-1      Fw

<400>  5
ggggatcctt tcgccacaat cagagagta                                         29


<210>  6
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rap-1      Rv

<400>  6
ggggtaccgt accgaagaac ttggcgttc                                         29


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hsp20     Fw

<400>  7
ctcgagtgca acaacgccga ccag                                              24


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hsp20     Rv

<400>  8
ctgcagaatc tgaacgcggc gcatc                                             25


<210>  9
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MABbo   Fw

<400>  9
ggggcgctag catgataacc gttgca                                            26


<210>  10
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MABbo   Rv

<400>  10
ggggcgctag caatctgaac gcggcgg                                           27


<210>  11
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TK  1

<400>  11
tccccgcggt gaacggcgga catattc                                           27


<210>  12
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TK  4

<400>  12
ggggtacctt atgagccgac gtaaca                                            26


<210>  13
<211>  656
<212>  DNA
<213>  Babesia bovis

<400>  13
tttcacacca tgtgtctgct tttcgccaca atcagagagt aggaagtctc gctccagctg       60

aagtggtagg tgatttaacc tccacattgg aaacagctga tactttgatg actctccgtg      120

accacatgca caacattact aaggatatga aacatgtttt gagcaatggc cgtgagcaga      180

ttgtaaatga tgtttgctct aatgctcctg aggactccaa ctgtcgtgag gtagttaaca      240

attatgctga ccgttgtgaa atgtacggat gctttacgat tgacaatgtc aaatatccgt      300

tgtatcaaga gtaccaacct ctatctcttc caaaccctta ccagttggat gctgcgttca      360

gattgttcaa agagagtgca tccaatcctg ctaagaacag cgtaaaacgc gaatggttgc      420

gtttcagaaa tggagcgaac catggtgatt accactactt cgtcactggt ctgttgaaca      480

acaatgttgt gcacggggaa ggaactaccg atgttgaata tcttgtcaac aaggtactct      540

atatggctac catgaactac aagacttatt tgacagtaaa cagtatgaac gccaagtttt      600

tcaacagatt cagcttcact acaaagatat tcagccgtcg tattaggcaa acattg          656


<210>  14
<211>  656
<212>  DNA
<213>  Babesia bovis

<400>  14
taaccgttgc attctgctcc atccttttca cttatacatt ggcatctcca caagagaaga       60

ctgtaccaac taaacaggtg aatgggagtc atttactgtc tgatgatatg aaaatgtttt      120

atgatgtaat gcgcagcatt gatgaaagca tgttgaaaag cattcttgag aagaattttg      180

aggctgttgg gatggaagct caatcggcta ctaaaacaca tgacgctctg aaggctgtaa      240

aacaattaat caaaaccgat gcacctttca acacatcgga ctttgacaca ctcgatttgg      300

aatatctctc agggcaatct aatgaagagc tgttgaagct tctgattgag gcaatatatg      360

gtatggaaat cataattgaa aaaacccact ctttcgtggg agagagtcaa aaatattccg      420

atgacgtaga caccgagttg agaaagtatt acaaggctaa tatttacgat gaccagagtg      480

aatataataa ggacaaatta agcaaccttt acaaggcatt catcactgat tctggcgctt      540

tgagaatcgc atcggaggaa cttataaaat tcgaaacaag gaaagctcaa aagaatgact      600

acagatttat caatccttct tcaacttcgg aagccgaaac accctctcca tcgtct          656


<210>  15
<211>  534
<212>  DNA
<213>  Babesia bovis

<400>  15
atgtcgtgta ttatgaggtg caacaacgcc gaccaggagg tcatcatcga cgagcagact       60

ggtctcccta tcaagagcca cgactacagt gagaagccat ctgtcatcta caagccttct      120

accactgtcc cacagaacac tcttcttgag attcctcctc ctaaggagtt ggagaacccg      180

attactttca acccaactgt ggacactttc tttgatgctg acaacaacaa attggttttg      240

ttgatggaac ttcctggttt cagcagcact gacatcaatg ttgagtgtgg ttggggtgaa      300

ttgatcatca gcggccctcg taacaaggat gagctttacg agaaattcgg taacaacttg      360

gacattcaca ttcgtgagcg taaggttggt tacttctaca ggcgtttcaa gcttcccaac      420

aacgccattg acaagagcat tagcgttggc tactctaatg gtattttgga catcaggatc      480

gagtgctctc aattctctga gatgcgccgc gttcagattg acgccaaggc ctaa            534


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter

<400>  16
taatacgact cactataggg gcgg                                              24


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13   Fw

<400>  17
ggggcgctag catgataacc gttgca                                            26


