                         SEQUENCE LISTING

<110>  The Scripps Research Institute
 
<120>  Methods and Compositions for Producing Activated Natural Killer 
       Cells and Related Uses

<130>  1708.2PC

<140>  PCT/US2016/038338
<141>  2016-06-18

<150>  US 62/243,314
<151>  2015-10-19

<150>  US 62/182,386
<151>  2015-06-19

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  243
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  1

Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Arg Lys Val 
1               5                   10                  15      


Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Arg Asp Thr Phe Asn 
            20                  25                  30          


Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asp Tyr Ala Gln 
    50                  55                  60                  


Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Asp Arg Lys Leu Gly Gly Ser Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser 
    130                 135                 140                 


Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr Gln Gln Glu Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Thr Leu Gln Arg Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Asn Ser Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Arg 
            


<210>  2
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  2

Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Arg Lys Val 
1               5                   10                  15      


Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Arg Asp Thr Phe Asn 
            20                  25                  30          


Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asp Tyr Ala Gln 
    50                  55                  60                  


Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Asp Arg Lys Leu Gly Gly Ser Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  3
<211>  108
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  3

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Ser Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  4
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  4

Arg Asp Thr Phe Asn Thr Tyr Gly 
1               5               


<210>  5
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  5

Ile Ile Pro Ile Phe Gly Thr Ala 
1               5               


<210>  6
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  6

Cys Ala Arg Asp Arg Lys Leu Gly Gly Ser Asp Tyr Trp 
1               5                   10              


<210>  7
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  7

Gln Gly Leu Gly Arg Trp 
1               5       


<210>  8
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  8

Gln Gln Ser Asn Ser Phe Pro Trp Thr 
1               5                   


<210>  9
<211>  258
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  9

Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Arg Lys Val 
1               5                   10                  15      


Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Arg Asp Thr Phe Asn 
            20                  25                  30          


Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asp Tyr Ala Gln 
    50                  55                  60                  


Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Asp Arg Arg Ala Gly Gly Ser Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser 
    130                 135                 140                 


Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr Gln Gln Glu Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Thr Leu Gln Arg Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Arg Arg Asn Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Arg Gly Leu Gly Gly Leu Glu Gln Lys Leu Ile Ser Glu Glu 
                245                 250                 255     


Asp Leu 
        


<210>  10
<211>  263
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  10

Ala Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      


Glu Val Arg Lys Val Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25                  30          


Arg Asp Thr Phe Asn Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro 
        35                  40                  45              


Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr 
    50                  55                  60                  


Ala Asp Tyr Ala Gln Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp 
65                  70                  75                  80  


Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Arg Arg Ala Gly Gly Ser 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr 
                165                 170                 175     


Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser 
            180                 185                 190         


Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln Gln Asn Asn His Asn Pro Trp Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Leu Gly Gly Leu Glu Gln Lys 
                245                 250                 255     


Leu Ile Ser Glu Glu Asp Leu 
            260             


<210>  11
<211>  263
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  11

Ala Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      


Glu Val Arg Lys Val Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25                  30          


Arg Asp Thr Phe Asn Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro 
        35                  40                  45              


Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr 
    50                  55                  60                  


Ala Asp Tyr Ala Gln Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp 
65                  70                  75                  80  


Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Arg Ala Leu Gly Gly Ser 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr 
                165                 170                 175     


Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser 
            180                 185                 190         


Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln Gln Ala Met His Arg Pro Trp Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Leu Gly Gly Leu Glu Gln Lys 
                245                 250                 255     


Leu Ile Ser Glu Glu Asp Leu 
            260             


<210>  12
<211>  263
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  12

Ala Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      


Glu Val Arg Lys Val Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25                  30          


Arg Asp Thr Phe Asn Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro 
        35                  40                  45              


Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr 
    50                  55                  60                  


Ala Asp Tyr Ala Gln Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp 
65                  70                  75                  80  


Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Arg Arg Ser Gly Gly Ser 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr 
                165                 170                 175     


Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser 
            180                 185                 190         


Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln Gln Thr Arg Arg Arg Pro Trp Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Leu Gly Gly Leu Glu Gln Lys 
                245                 250                 255     


Leu Ile Ser Glu Glu Asp Leu 
            260             


<210>  13
<211>  263
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  13

Ala Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      


Glu Val Arg Lys Val Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25                  30          


Arg Asp Thr Phe Asn Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro 
        35                  40                  45              


Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr 
    50                  55                  60                  


Ala Asp Tyr Ala Gln Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp 
65                  70                  75                  80  


Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Arg Arg Thr Gly Gly Ser 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Lys Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp Leu Ala Trp Tyr 
                165                 170                 175     


Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser 
            180                 185                 190         


Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln Gln Thr Lys Trp Pro Pro Trp Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Leu Gly Gly Leu Glu Gln Lys 
                245                 250                 255     


Leu Ile Ser Glu Glu Asp Leu 
            260             


<210>  14
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  14

Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Arg Lys Val 
1               5                   10                  15      


Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Arg Asp Thr Phe Asn 
            20                  25                  30          


Thr Tyr Gly Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asp Tyr Ala Gln 
    50                  55                  60                  


Lys Phe Arg Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Asp Arg Arg Ser Gly Gly Ser Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  15
<211>  108
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Leu Gly Arg Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Glu Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Gln Arg Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Arg Arg Arg Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  16
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  16

Cys Ala Arg Asp Arg Arg Ala Gly Gly Ser Asp Tyr Trp 
1               5                   10              


<210>  17
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  17

Gln Gly Leu Gly Arg Trp 
1               5       


<210>  18
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  18

Gln Gln Asn Asn His Asn Pro Trp Thr 
1               5                   


<210>  19
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  19

Cys Ala Arg Asp Arg Ala Leu Gly Gly Ser Asp Tyr Trp 
1               5                   10              


<210>  20
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  20

Gln Gln Ala Met His Arg Pro Trp Thr 
1               5                   


<210>  21
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  21

Cys Ala Arg Asp Arg Arg Ser Gly Gly Ser Asp Tyr Trp 
1               5                   10              


<210>  22
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  22

Gln Gln Thr Arg Arg Arg Pro Trp Thr 
1               5                   


<210>  23
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  23

Cys Ala Arg Asp Arg Arg Thr Gly Gly Ser Asp Tyr Trp 
1               5                   10              


<210>  24
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  24

Gln Gln Thr Lys Trp Pro Pro Trp Thr 
1               5                   


<210>  25
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  25

Ala Ala Ser 
1           


