                         SEQUENCE LISTING

<110>  WILLERT, Karl
       CARSON, Dennis
 
<120>  FZD7 SPECIFIC ANTIBODIES AND VACCINES TO TREAT CANCER AND CONTROL
        STEM CELL FUNCTION

<130>  41112.2213

<140>  PCT/US16/037919
<141>  2016-06-16

<150>  62180359
<151>  2015-06-16

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  469
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Asp Pro Gly Ala Ala Ala Pro Leu Ser Ser Leu Gly Leu Cys 
1               5                   10                  15      


Ala Leu Val Leu Ala Leu Leu Gly Ala Leu Ser Ala Gly Ala Gly Ala 
            20                  25                  30          


Gln Pro Tyr His Gly Glu Lys Gly Ile Ser Val Pro Asp His Gly Phe 
        35                  40                  45              


Cys Gln Pro Ile Ser Ile Pro Leu Cys Thr Asp Ile Ala Tyr Asn Gln 
    50                  55                  60                  


Thr Leu Glu Val His Gln Phe Tyr Pro Leu Val Lys Val Gln Cys Ser 
65                  70                  75                  80  


Pro Glu Leu Arg Phe Phe Leu Cys Ser Met Tyr Ala Pro Val Cys Thr 
                85                  90                  95      


Val Leu Asp Gln Ala Ile Pro Pro Cys Arg Ser Leu Cys Glu Arg Ala 
            100                 105                 110         


Arg Gln Gly Cys Glu Ala Leu Met Asn Lys Phe Gly Phe Gln Trp Pro 
        115                 120                 125             


Glu Arg Val Gly Gln Asn Thr Ser Asp Gly Ser Gly Gly Pro Gly Gly 
    130                 135                 140                 


Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro Asp Leu Pro Phe 
145                 150                 155                 160 


Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly Arg Pro Ala Phe 
                165                 170                 175     


Pro Phe Ser Cys Pro Arg Gln Leu Lys Val Pro Pro Tyr Leu Gly Tyr 
            180                 185                 190         


Arg Phe Leu Gly Leu Met Tyr Phe Lys Glu Glu Glu Arg Arg Phe Ala 
        195                 200                 205             


Arg Leu Trp Val Gly Val Trp Ser Val Leu Cys Cys Ala Ser Thr Leu 
    210                 215                 220                 


Phe Thr Val Leu Thr Tyr Leu Val Asp Met Arg Arg Phe Ser Tyr Pro 
225                 230                 235                 240 


Glu Arg Pro Ile Ile Phe Leu Ser Gly Cys Tyr Phe Met Val Ala Val 
                245                 250                 255     


Ala His Val Ala Asp Asp Gly Tyr Arg Thr Val Ala Gln Gly Thr Lys 
            260                 265                 270         


Lys Glu Gly Cys Thr Ile Leu Phe Met Val Leu Tyr Phe Phe Gly Met 
        275                 280                 285             


Ala Ser Ser Ile Trp Trp Val Ile Leu Ser Leu Thr Trp Phe Leu Ala 
    290                 295                 300                 


Ala Gly Met Lys Trp Gly His Glu Ala Ile Glu Ala Asn Ser Gln Tyr 
305                 310                 315                 320 


Phe His Leu Ala Ala Gln Val Asp Gly Asp Leu Leu Ser Gly Val Cys 
                325                 330                 335     


Tyr Val Gly Leu Ser Ser Val Asp Ala Leu Arg Gly Phe Val Leu Ala 
            340                 345                 350         


Pro Leu Phe Val Tyr Leu Phe Ile Gly Thr Ser Phe Leu Leu Ala Gly 
        355                 360                 365             


Phe Val Ser Leu Phe Arg Ile Arg Thr Ile Met Lys His Asp Gly Thr 
    370                 375                 380                 


Lys Thr Glu Lys Leu Glu Pro Ala Thr Ile Val Leu Ala Cys Tyr Phe 
385                 390                 395                 400 


Tyr Glu Gln Ala Phe Arg Glu His Trp Glu Arg Thr Trp Leu Leu Gln 
                405                 410                 415     


Thr Cys Lys Ser Tyr Ala Val Pro Cys Pro Pro Gly His Phe Pro Pro 
            420                 425                 430         


Met Ser Pro Asp Phe Thr Val Phe Met Ile Lys Tyr Leu Met Thr Met 
        435                 440                 445             


Ile Val Gly Ile Thr Thr Gly Tyr His Arg Leu Ser His Ser Ser Lys 
    450                 455                 460                 


Gly Glu Thr Ala Val 
465                 


<210>  2
<211>  467
<212>  PRT
<213>  Mus musculus

<400>  2

Met Arg Gly Pro Gly Thr Ala Ala Ser His Ser Pro Leu Gly Leu Cys 
1               5                   10                  15      


Ala Leu Val Leu Ala Leu Leu Gly Ala Leu Pro Thr Asp Thr Arg Ala 
            20                  25                  30          


Gln Pro Tyr His Gly Glu Lys Gly Ile Ser Val Pro Asp His Gly Phe 
        35                  40                  45              


Cys Gln Pro Ile Ser Ile Pro Leu Cys Thr Asp Ile Ala Tyr Asn Gln 
    50                  55                  60                  


Thr Leu Glu Val His Gln Phe Tyr Pro Leu Val Lys Val Gln Cys Ser 
65                  70                  75                  80  


Pro Glu Leu Arg Phe Phe Leu Cys Ser Met Tyr Ala Pro Val Cys Thr 
                85                  90                  95      


Val Leu Asp Gln Ala Ile Pro Pro Cys Arg Ser Leu Cys Glu Arg Ala 
            100                 105                 110         


Arg Gln Gly Cys Glu Ala Leu Met Asn Lys Phe Gly Phe Gln Trp Pro 
        115                 120                 125             


Glu Arg Val Gly Gln Asn Thr Ser Asp Gly Ser Gly Gly Ala Gly Gly 
    130                 135                 140                 


Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro Asp Pro Pro Phe 
145                 150                 155                 160 


Thr Ala Met Ser Pro Ser Asp Gly Arg Gly Arg Leu Ser Phe Pro Phe 
                165                 170                 175     


Ser Cys Pro Arg Gln Leu Lys Val Pro Pro Tyr Leu Gly Tyr Arg Phe 
            180                 185                 190         


Leu Gly Leu Met Tyr Phe Lys Glu Glu Glu Arg Arg Phe Ala Arg Leu 
        195                 200                 205             


Trp Val Gly Val Trp Ser Val Leu Cys Cys Ala Ser Thr Leu Phe Thr 
    210                 215                 220                 


Val Leu Thr Tyr Leu Val Asp Met Arg Arg Phe Ser Tyr Pro Glu Arg 
225                 230                 235                 240 


Pro Ile Ile Phe Leu Ser Gly Cys Tyr Phe Met Val Ala Val Ala His 
                245                 250                 255     


Val Ala Asp Asp Gly Tyr Arg Thr Val Ala Gln Gly Thr Lys Lys Glu 
            260                 265                 270         


Gly Cys Thr Ile Leu Phe Met Val Leu Tyr Phe Phe Gly Met Ala Ser 
        275                 280                 285             


Ser Ile Trp Trp Val Ile Leu Ser Leu Thr Trp Phe Leu Ala Ala Gly 
    290                 295                 300                 


Met Lys Trp Gly His Glu Ala Ile Glu Ala Asn Ser Gln Tyr Phe His 
305                 310                 315                 320 


Leu Ala Ala Gln Val Asp Gly Asp Leu Leu Ser Gly Val Cys Tyr Val 
                325                 330                 335     


Gly Leu Ser Ser Val Asp Ala Leu Arg Gly Phe Val Leu Ala Pro Leu 
            340                 345                 350         


Phe Val Tyr Leu Phe Ile Gly Thr Ser Phe Leu Leu Ala Gly Phe Val 
        355                 360                 365             


Ser Leu Phe Arg Ile Arg Thr Ile Met Lys His Asp Gly Thr Lys Thr 
    370                 375                 380                 


Glu Lys Leu Glu Pro Ala Thr Ile Val Leu Ala Cys Tyr Phe Tyr Glu 
385                 390                 395                 400 


Gln Ala Phe Arg Glu His Trp Glu Arg Thr Trp Leu Leu Gln Thr Cys 
                405                 410                 415     


Lys Ser Tyr Ala Val Pro Cys Pro Pro Gly His Phe Ser Pro Met Ser 
            420                 425                 430         


Pro Asp Phe Thr Val Phe Met Ile Lys Tyr Leu Met Thr Met Ile Val 
        435                 440                 445             


Gly Ile Thr Thr Gly Tyr His Arg Leu Ser His Ser Ser Lys Gly Glu 
    450                 455                 460                 


Thr Ala Val 
465         


<210>  3
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of Human FZD7 and Mouse Fzd7


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa may be Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa may be Pro or Ser

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa may be Leu or His

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa may be Ser or Pro

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa may be Ser or Pro

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa may be Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa may be Gly or Asp

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa may be Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Xaa may be Gly or Arg

<220>
<221>  MISC_FEATURE
<222>  (142)..(142)
<223>  Xaa may be Pro or Ala

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa may be Gly or Ser

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa may be Leu or Pro

<220>
<221>  MISC_FEATURE
<222>  (163)..(163)
<223>  Xaa may be Leu or Met

<220>
<221>  MISC_FEATURE
<222>  (164)..(164)
<223>  Xaa may be Pro or Ser

<220>
<221>  MISC_FEATURE
<222>  (166)..(166)
<223>  Xaa may be Gly or absent

<220>
<221>  MISC_FEATURE
<222>  (167)..(167)
<223>  Xaa may be Ala or absent

<220>
<221>  MISC_FEATURE
<222>  (174)..(174)
<223>  Xaa may be Pro or Leu

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa may be Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (431)..(431)
<223>  Xaa may be Pro Ser

<400>  3

Met Arg Xaa Pro Gly Xaa Ala Ala Xaa Xaa Ser Xaa Leu Gly Leu Cys 
1               5                   10                  15      


Ala Leu Val Leu Ala Leu Leu Gly Ala Leu Xaa Xaa Xaa Xaa Xaa Ala 
            20                  25                  30          


Gln Pro Tyr His Gly Glu Lys Gly Ile Ser Val Pro Asp His Gly Phe 
        35                  40                  45              


Cys Gln Pro Ile Ser Ile Pro Leu Cys Thr Asp Ile Ala Tyr Asn Gln 
    50                  55                  60                  


Thr Leu Glu Val His Gln Phe Tyr Pro Leu Val Lys Val Gln Cys Ser 
65                  70                  75                  80  


Pro Glu Leu Arg Phe Phe Leu Cys Ser Met Tyr Cys Ala Pro Val Cys 
                85                  90                  95      


Thr Val Leu Asp Gln Ala Ile Pro Pro Cys Arg Ser Leu Cys Glu Arg 
            100                 105                 110         


Ala Arg Gln Gly Cys Glu Ala Leu Met Asn Lys Phe Gly Phe Gln Trp 
        115                 120                 125             


Pro Glu Arg Val Gly Gln Asn Thr Ser Asp Ser Gly Gly Xaa Gly Gly 
    130                 135                 140                 


Xaa Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro Asp Xaa Pro Phe 
145                 150                 155                 160 


Thr Ala Xaa Xaa Pro Xaa Xaa Ser Asp Gly Arg Gly Arg Xaa Xaa Phe 
                165                 170                 175     


Pro Phe Ser Cys Pro Arg Gln Leu Lys Val Pro Pro Tyr Leu Gly Tyr 
            180                 185                 190         


Arg Phe Leu Gly Leu Met Tyr Phe Lys Glu Glu Glu Arg Arg Phe Ala 
        195                 200                 205             


Arg Leu Trp Val Gly Val Trp Ser Val Leu Cys Cys Ala Ser Thr Leu 
    210                 215                 220                 


Phe Thr Val Leu Thr Tyr Leu Val Asp Met Arg Arg Phe Ser Tyr Pro 
225                 230                 235                 240 


Glu Arg Pro Ile Ile Phe Leu Ser Gly Cys Tyr Phe Met Val Ala Val 
                245                 250                 255     


Ala His Val Ala Asp Asp Gly Tyr Arg Thr Val Ala Gln Gly Thr Lys 
            260                 265                 270         


Lys Glu Gly Cys Thr Ile Leu Phe Met Val Leu Tyr Phe Phe Gly Met 
        275                 280                 285             


Ala Ser Ser Ile Trp Trp Val Ile Leu Ser Leu Thr Trp Phe Leu Ala 
    290                 295                 300                 


Ala Gly Met Lys Trp Gly His Glu Ala Ile Glu Ala Asn Ser Gln Tyr 
305                 310                 315                 320 


Phe His Leu Ala Ala Gln Val Asp Gly Asp Leu Leu Ser Gly Val Cys 
                325                 330                 335     


Tyr Val Gly Leu Ser Ser Val Asp Ala Leu Arg Gly Phe Val Leu Ala 
            340                 345                 350         


Pro Leu Phe Val Tyr Leu Phe Ile Gly Thr Ser Phe Leu Leu Ala Gly 
        355                 360                 365             


Phe Val Ser Leu Phe Arg Ile Arg Thr Ile Met Lys His Asp Gly Thr 
    370                 375                 380                 


Lys Thr Glu Lys Leu Glu Pro Ala Thr Ile Val Leu Ala Cys Tyr Phe 
385                 390                 395                 400 


Tyr Glu Gln Ala Phe Arg Glu His Trp Glu Arg Thr Trp Leu Leu Gln 
                405                 410                 415     


Thr Cys Lys Ser Tyr Ala Val Pro Cys Pro Pro Gly His Phe Xaa Pro 
            420                 425                 430         


Met Ser Pro Asp Phe Thr Val Phe Met Ile Lys Tyr Leu Met Thr Met 
        435                 440                 445             


Ile Val Gly Ile Thr Thr Gly Tyr His Arg Leu Ser His Ser Ser Lys 
    450                 455                 460                 


Gly Glu Thr Ala Val 
465                 


<210>  4
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7-Fab-1791 Light chain

<400>  4

Asp Ile Val Met Thr Gln Ser Pro Lys Ser Met Ser Met Ser Val Gly 
1               5                   10                  15      


Glu Arg Val Thr Leu Arg Cys Lys Ala Ser Glu Asn Val Leu Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Ser Tyr Arg Tyr Pro Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro 
            100                 105                 110         


Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly Gly 
        115                 120                 125             


Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile Asn 
    130                 135                 140                 


Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu Asn 
145                 150                 155                 160 


Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr Thr 
            180                 185                 190         


Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser Phe 
        195                 200                 205             


Asn Arg Asn Glu Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser 
    210                 215                 220             


<210>  5
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7-Fab-1791 Heavy chain

<400>  5

Glu Val Gln Pro Val Glu Ser Gly Gly Gly Leu Val Gln Pro Lys Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Lys Asn Tyr Asp Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln Ser Met 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Met Tyr 
                85                  90                  95      


Tyr Cys Val Arg Glu Asn Tyr Gly Gly Arg Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
        115                 120                 125             


Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr 
145                 150                 155                 160 


Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser 
            180                 185                 190         


Ser Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala 
        195                 200                 205             


Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Ser His His 
    210                 215                 220                 


His His His His His 
225                 


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of FZD7-Fab-1791 Light chain

<400>  6

Lys Ala Ser Glu Asn Val Leu Asn Tyr Val Ser 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of FZD7-Fab-1791 Light chain

<400>  7

Gly Ala Ser Asn Arg Tyr Thr 
1               5           


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of FZD7-Fab-1791 Light chain

<400>  8

Gly Gln Ser Tyr Arg Tyr Pro 
1               5           


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of FZD7-Fab-1791 Heavy chain

<400>  9

Thr Tyr Ala Met His 
1               5   


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of FZD7-Fab-1791 Heavy chain

<400>  10

Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Lys Asn Tyr Asp Asp Ser 
1               5                   10                  15      


Val Lys Asp 
            


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of FZD7-Fab-1791 Heavy chain

<400>  11

Glu Asn Tyr Gly Gly Arg Phe Asp Tyr 
1               5                   


<210>  12
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7-Fab-1291 Light chain

<400>  12

Asp Val Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Lys Ala Lys Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Ser Tyr Pro Tyr Asp Val 
    210                 215                 220                 


Pro Asp Tyr Ala Ser 
225                 


<210>  13
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7-Fab-1291 Heavy chain

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Asp Gly Gly Ser Tyr Thr Arg Tyr Pro Asp Lys Leu 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser His Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Gly Arg Arg Asp Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
145                 150                 155                 160 


Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser 
            180                 185                 190         


Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser 
        195                 200                 205             


Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys His His His 
    210                 215                 220                 


His His His His 
225             


<210>  14
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of FZD7-Fab-1291 Light chain

<400>  14

Lys Ser Ser Gln Ser Leu Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala Trp 
        


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of FZD7-Fab-1291 Light chain

<400>  15

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of FZD7-Fab-1291 Light chain

<400>  16

Gln Gln Tyr Tyr Ser Tyr Pro 
1               5           


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of FZD7-Fab-1291 Heavy chain

<400>  17

Ser Tyr Ala Met Ser 
1               5   


<210>  18
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of FZD7-Fab-1291 Heavy chain

<400>  18

Thr Ile Ser Asp Gly Gly Ser Tyr Thr Arg Tyr Pro Asp Lys Leu Lys 
1               5                   10                  15      


Gly 
    


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of FZD7-Fab-1291 Heavy chain

<400>  19

Val Gly Gly Arg Arg Asp Tyr Phe Asp Tyr 
1               5                   10  


<210>  20
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chFZD7-1791 Light chain

<400>  20

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Lys Cys Asp Ile Val Met Thr Gln Ser Pro Lys Ser 
            20                  25                  30          


Met Ser Met Ser Val Gly Glu Arg Val Thr Leu Arg Cys Lys Ala Ser 
        35                  40                  45              


Glu Asn Val Leu Asn Tyr Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln 
    50                  55                  60                  


Ser Pro Lys Leu Leu Ile Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val 
65                  70                  75                  80  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Asp Tyr His Cys Gly Gln 
            100                 105                 110         


Ser Tyr Arg Tyr Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
        115                 120                 125             


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
    130                 135                 140                 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
145                 150                 155                 160 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
                165                 170                 175     


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
            180                 185                 190         


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        195                 200                 205             


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
    210                 215                 220                 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235 


<210>  21
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chFZD7-1791 Heavy chain

<400>  21

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Lys Cys Glu Val Gln Pro Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Lys Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Asn Thr Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr 
65                  70                  75                  80  


Ala Lys Asn Tyr Asp Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg 
                85                  90                  95      


Asp Asp Ser Gln Ser Met Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr 
            100                 105                 110         


Glu Asp Thr Ala Met Tyr Tyr Cys Val Arg Glu Asn Tyr Gly Gly Arg 
        115                 120                 125             


Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
145                 150                 155                 160 


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
    210                 215                 220                 


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
225                 230                 235                 240 


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                245                 250                 255     


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470         


<210>  22
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chFZD7-1291 Light chain

<400>  22

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Lys Cys Asp Val Val Met Ser Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ala Val Ser Val Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser 
        35                  40                  45              


Gln Ser Leu Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser 
65                  70                  75                  80  


Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Lys Ala Lys Asp Leu Ala 
            100                 105                 110         


Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Thr Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
    130                 135                 140                 


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
145                 150                 155                 160 


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
                165                 170                 175     


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
            180                 185                 190         


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
        195                 200                 205             


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
    210                 215                 220                 


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
225                 230                 235                 240 


Cys 
    


<210>  23
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chFZD7-1291 Heavy chain

<400>  23

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Lys Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu 
    50                  55                  60                  


Lys Arg Leu Glu Trp Val Ala Thr Ile Ser Asp Gly Gly Ser Tyr Thr 
65                  70                  75                  80  


Arg Tyr Pro Asp Lys Leu Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Asn Leu Tyr Leu Gln Met Ser His Leu Lys Ser Glu Asp 
            100                 105                 110         


Thr Ala Met Tyr Tyr Cys Ala Arg Val Gly Gly Arg Arg Asp Tyr Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr 
    130                 135                 140                 


Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
145                 150                 155                 160 


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
                165                 170                 175     


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
            180                 185                 190         


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
        195                 200                 205             


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
    210                 215                 220                 


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu 
225                 230                 235                 240 


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
                245                 250                 255     


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    290                 295                 300                 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
305                 310                 315                 320 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        355                 360                 365             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    370                 375                 380                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
385                 390                 395                 400 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                405                 410                 415     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            420                 425                 430         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        435                 440                 445             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    450                 455                 460                 


Leu Ser Leu Ser Pro Gly Lys 
465                 470     


<210>  24
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7

<400>  24

Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Pro Ala Phe 
        35      


<210>  25
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mFzd7

<400>  25

Gly Ala Gly Gly Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Pro Pro Phe Thr Ala Met Ser Pro Ser Asp Gly Arg Gly Arg Leu 
            20                  25                  30          


Ser Phe 
        


<210>  26
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized mFzd7

<400>  26

Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Pro Ala Phe 
        35      


<210>  27
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-3P

<400>  27

Gly Ala Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Leu Ser Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Leu Ala Phe 
        35      


<210>  28
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mFzd7-GA

<400>  28

Gly Ala Gly Gly Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Pro Pro Phe Thr Ala Met Ser Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Leu Ser Phe 
        35      


<210>  29
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-LP to MS

<400>  29

Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Met Ser Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Pro Ala Phe 
        35      


<210>  30
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-LPPGA to MSP

<400>  30

Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Met Ser Pro Ser Asp Gly Arg Gly Arg Pro 
            20                  25                  30          


Ala Phe 
        


<210>  31
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-PA to LS

<400>  31

Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Leu Ser Phe 
        35      


<210>  32
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-PGGG to AGGS

<400>  32

Gly Ala Gly Gly Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Pro Ala Phe 
        35      


<210>  33
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFZD7-L188P

<400>  33

Gly Ala Gly Gly Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Pro Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly 
            20                  25                  30          


Arg Pro Ala Phe 
        35      


<210>  34
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mFzd7-P188L

<400>  34

Gly Ala Gly Gly Ser Pro Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro 
1               5                   10                  15      


Asp Leu Pro Phe Thr Ala Met Ser Pro Ser Asp Gly Arg Gly Arg Leu 
            20                  25                  30          


Ser Phe 
        


<210>  35
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7 wildtype fragment

<400>  35

Gly Ser Gly Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro 
1               5                   10                  15      


Tyr Leu Pro Asp Leu Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp 
            20                  25                  30          


Gly Arg Gly Arg Pro Ala Phe Pro Phe 
        35                  40      


<210>  36
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FZD7 mutant fragment

<400>  36

Gly Ser Gly Gly Pro Gly Gly Gly Pro Thr Ala Tyr Pro Thr Ala Pro 
1               5                   10                  15      


Tyr Leu Pro Asp Pro Pro Phe Thr Ala Leu Pro Pro Gly Ala Ser Asp 
            20                  25                  30          


Gly Arg Gly Arg Pro Ala Phe Pro Phe 
        35                  40      


<210>  37
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR/Cas9 Repair Oligo

<400>  37
gacggctccg ggggcgcggg cggcagtccc accgcctacc ctactgctcc ctacctgcca       60

gatctacctt tcactgcgat gtccccctca gatggcagag gccgcttgtc tttccccttc      120

tcgtgtccgc gc                                                          132


<210>  38
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer (Forward)

<400>  38
tggggcagaa cacgtccg                                                     18


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer (Reverse)

<400>  39
gaagagcgtc gaggcgcagc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing Primer

<400>  40
gctcacctag gaagcggtag                                                   20


<210>  41
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo sequence to generate peptide with L188

<400>  41
gaattccggc tccgggggcc caggcggcgg ccccactgcc taccctaccg cgccctacct       60

gccggacctg cccttcaccg cgctgccccc gggggcctca gatggcaggg ggcgtcccgc      120

cttccccttc tcctcgag                                                    138


<210>  42
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo sequence to generate peptide with P188

<400>  42
gaattccggc tccgggggcc caggcggcgg ccccactgcc taccctaccg cgccctacct       60

gccggacccg cccttcaccg cgctgccccc gggggcctca gatggcaggg ggcgtcccgc      120

cttccccttc tcctcgag                                                    138


<210>  43
<211>  542
<212>  PRT
<213>  Homo sapiens

<400>  43

Gln Pro Tyr His Gly Glu Lys Gly Ile Ser Val Pro Asp His Gly Phe 
1               5                   10                  15      


Cys Gln Pro Ile Ser Ile Pro Leu Cys Thr Asp Ile Ala Tyr Asn Gln 
            20                  25                  30          


Thr Ile Leu Pro Asn Leu Leu Gly His Thr Asn Gln Glu Asp Ala Gly 
        35                  40                  45              


Leu Glu Val His Gln Phe Tyr Pro Leu Val Lys Val Gln Cys Ser Pro 
    50                  55                  60                  


Glu Leu Arg Phe Phe Leu Cys Ser Met Tyr Ala Pro Val Cys Thr Val 
65                  70                  75                  80  


Leu Asp Gln Ala Ile Pro Pro Cys Arg Ser Leu Cys Glu Arg Ala Arg 
                85                  90                  95      


Gln Gly Cys Glu Ala Leu Met Asn Lys Phe Gly Phe Gln Trp Pro Glu 
            100                 105                 110         


Arg Leu Arg Cys Glu Asn Phe Pro Val His Gly Ala Gly Glu Ile Cys 
        115                 120                 125             


Val Gly Gln Asn Thr Ser Asp Gly Ser Gly Gly Pro Gly Gly Gly Pro 
    130                 135                 140                 


Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro Asp Leu Pro Phe Thr Ala 
145                 150                 155                 160 


Leu Pro Pro Gly Ala Ser Asp Gly Arg Gly Arg Pro Ala Phe Pro Phe 
                165                 170                 175     


Ser Cys Pro Arg Gln Leu Lys Val Pro Pro Tyr Leu Gly Tyr Arg Phe 
            180                 185                 190         


Leu Gly Glu Arg Asp Cys Gly Ala Pro Cys Glu Pro Gly Arg Ala Asn 
        195                 200                 205             


Gly Leu Met Tyr Phe Lys Glu Glu Glu Arg Arg Phe Ala Arg Leu Trp 
    210                 215                 220                 


Val Gly Val Trp Ser Val Leu Cys Cys Ala Ser Thr Leu Phe Thr Val 
225                 230                 235                 240 


Leu Thr Tyr Leu Val Asp Met Arg Arg Phe Ser Tyr Pro Glu Arg Pro 
                245                 250                 255     


Ile Ile Phe Leu Ser Gly Cys Tyr Phe Met Val Ala Val Ala His Val 
            260                 265                 270         


Ala Gly Phe Leu Leu Glu Asp Arg Ala Val Cys Val Glu Arg Phe Ser 
        275                 280                 285             


Asp Asp Gly Tyr Arg Thr Val Ala Gln Gly Thr Lys Lys Glu Gly Cys 
    290                 295                 300                 


Thr Ile Leu Phe Met Val Leu Tyr Phe Phe Gly Met Ala Ser Ser Ile 
305                 310                 315                 320 


Trp Trp Val Ile Leu Ser Leu Thr Trp Phe Leu Ala Ala Gly Met Lys 
                325                 330                 335     


Trp Gly His Glu Ala Ile Glu Ala Asn Ser Gln Tyr Phe His Leu Ala 
            340                 345                 350         


Ala Trp Ala Val Pro Ala Val Lys Thr Ile Thr Ile Leu Ala Met Gly 
        355                 360                 365             


Gln Val Asp Gly Asp Leu Leu Ser Gly Val Cys Tyr Val Gly Leu Ser 
    370                 375                 380                 


Ser Val Asp Ala Leu Arg Gly Phe Val Leu Ala Pro Leu Phe Val Tyr 
385                 390                 395                 400 


Leu Phe Ile Gly Thr Ser Phe Leu Leu Ala Gly Phe Val Ser Leu Phe 
                405                 410                 415     


Arg Ile Arg Thr Ile Met Lys His Asp Gly Thr Lys Thr Glu Lys Leu 
            420                 425                 430         


Glu Lys Leu Met Val Arg Ile Gly Val Phe Ser Val Leu Tyr Thr Val 
        435                 440                 445             


Pro Ala Thr Ile Val Leu Ala Cys Tyr Phe Tyr Glu Gln Ala Phe Arg 
    450                 455                 460                 


Glu His Trp Glu Arg Thr Trp Leu Leu Gln Thr Cys Lys Ser Tyr Ala 
465                 470                 475                 480 


Val Pro Cys Pro Pro Gly His Phe Pro Pro Met Ser Pro Asp Phe Thr 
                485                 490                 495     


Val Phe Met Ile Lys Tyr Leu Met Thr Met Ile Val Gly Ile Thr Thr 
            500                 505                 510         


Gly Phe Trp Ile Trp Ser Gly Lys Thr Leu Gln Ser Trp Arg Arg Phe 
        515                 520                 525             


Tyr His Arg Leu Ser His Ser Ser Lys Gly Glu Thr Ala Val 
    530                 535                 540         


<210>  44
<211>  540
<212>  PRT
<213>  Mus musculus

<400>  44

Gln Pro Tyr His Gly Glu Lys Gly Ile Ser Val Pro Asp His Gly Phe 
1               5                   10                  15      


Cys Gln Pro Ile Ser Ile Pro Leu Cys Thr Asp Ile Ala Tyr Asn Gln 
            20                  25                  30          


Thr Ile Leu Pro Asn Leu Leu Gly His Thr Asn Gln Glu Asp Ala Gly 
        35                  40                  45              


Leu Glu Val His Gln Phe Tyr Pro Leu Val Lys Val Gln Cys Ser Pro 
    50                  55                  60                  


Glu Leu Arg Phe Phe Leu Cys Ser Met Tyr Ala Pro Val Cys Thr Val 
65                  70                  75                  80  


Leu Asp Gln Ala Ile Pro Pro Cys Arg Ser Leu Cys Glu Arg Ala Arg 
                85                  90                  95      


Gln Gly Cys Glu Ala Leu Met Asn Lys Phe Gly Phe Gln Trp Pro Glu 
            100                 105                 110         


Arg Leu Arg Cys Glu Asn Phe Pro Val His Gly Ala Gly Glu Ile Cys 
        115                 120                 125             


Val Gly Gln Asn Thr Ser Asp Gly Ser Gly Gly Ala Gly Gly Ser Pro 
    130                 135                 140                 


Thr Ala Tyr Pro Thr Ala Pro Tyr Leu Pro Asp Pro Pro Phe Thr Ala 
145                 150                 155                 160 


Met Ser Pro Ser Asp Gly Arg Gly Arg Leu Ser Phe Pro Phe Ser Cys 
                165                 170                 175     


Pro Arg Gln Leu Lys Val Pro Pro Tyr Leu Gly Tyr Arg Phe Leu Gly 
            180                 185                 190         


Glu Arg Asp Cys Gly Ala Pro Cys Glu Pro Gly Arg Ala Asn Gly Leu 
        195                 200                 205             


Met Tyr Phe Lys Glu Glu Glu Arg Arg Phe Ala Arg Leu Trp Val Gly 
    210                 215                 220                 


Val Trp Ser Val Leu Cys Cys Ala Ser Thr Leu Phe Thr Val Leu Thr 
225                 230                 235                 240 


Tyr Leu Val Asp Met Arg Arg Phe Ser Tyr Pro Glu Arg Pro Ile Ile 
                245                 250                 255     


Phe Leu Ser Gly Cys Tyr Phe Met Val Ala Val Ala His Val Ala Gly 
            260                 265                 270         


Phe Leu Leu Glu Asp Arg Ala Val Cys Val Glu Arg Phe Ser Asp Asp 
        275                 280                 285             


Gly Tyr Arg Thr Val Ala Gln Gly Thr Lys Lys Glu Gly Cys Thr Ile 
    290                 295                 300                 


Leu Phe Met Val Leu Tyr Phe Phe Gly Met Ala Ser Ser Ile Trp Trp 
305                 310                 315                 320 


Val Ile Leu Ser Leu Thr Trp Phe Leu Ala Ala Gly Met Lys Trp Gly 
                325                 330                 335     


His Glu Ala Ile Glu Ala Asn Ser Gln Tyr Phe His Leu Ala Ala Trp 
            340                 345                 350         


Ala Val Pro Ala Val Lys Thr Ile Thr Ile Leu Ala Met Gly Gln Val 
        355                 360                 365             


Asp Gly Asp Leu Leu Ser Gly Val Cys Tyr Val Gly Leu Ser Ser Val 
    370                 375                 380                 


Asp Ala Leu Arg Gly Phe Val Leu Ala Pro Leu Phe Val Tyr Leu Phe 
385                 390                 395                 400 


Ile Gly Thr Ser Phe Leu Leu Ala Gly Phe Val Ser Leu Phe Arg Ile 
                405                 410                 415     


Arg Thr Ile Met Lys His Asp Gly Thr Lys Thr Glu Lys Leu Glu Lys 
            420                 425                 430         


Leu Met Val Arg Ile Gly Val Phe Ser Val Leu Tyr Thr Val Pro Ala 
        435                 440                 445             


Thr Ile Val Leu Ala Cys Tyr Phe Tyr Glu Gln Ala Phe Arg Glu His 
    450                 455                 460                 


Trp Glu Arg Thr Trp Leu Leu Gln Thr Cys Lys Ser Tyr Ala Val Pro 
465                 470                 475                 480 


Cys Pro Pro Gly His Phe Ser Pro Met Ser Pro Asp Phe Thr Val Phe 
                485                 490                 495     


Met Ile Lys Tyr Leu Met Thr Met Ile Val Gly Ile Thr Thr Gly Phe 
            500                 505                 510         


Trp Ile Trp Ser Gly Lys Thr Leu Gln Ser Trp Arg Arg Phe Tyr His 
        515                 520                 525             


Arg Leu Ser His Ser Ser Lys Gly Glu Thr Ala Val 
    530                 535                 540 


