                         SEQUENCE LISTING

<110>  Georgia Tech Research Corporation
       Mark , Mark  P.
       Watstein, Daniel  M.
       McNerney, Monica P.
 
<120>  PIGMENT-BASED MICRONUTRIENT BIOSENSORS

<130>  GTRC 6583

<150>  62/175,576
<151>  2015-06-15

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  129
<212>  DNA
<213>  artificial

<220>
<223>  synthetic transcription terminator

<400>  1
ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt       60

gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct      120

gcgtttata                                                              129


<210>  2
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  a synthetic, a weak constitutive transcriptional promoter and 
       assembly scar used to drive crtEBI

<400>  2
tactagagct gatggctagc tcagtcctag ggattatgct agc                         43


<210>  3
<211>  3393
<212>  DNA
<213>  artificial

<220>
<223>  coding sequences for crtEBI, genes used to produce the carotenoid
       lycopene with ribosomal binding sites and assembly scars

<400>  3
tactagagga ggtactagat gacggtctgc gcaaaaaaac acgttcatct cactcgcgat       60

gctgcggagc agttactggc tgatattgat cgacgccttg atcagttatt gcccgtggag      120

ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg cgctggcacc gggaaaacgt      180

attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg gttgcgctgt cagccatgac      240

ggattactgg atttggcctg tgcggtggaa atggtccacg cggcttcgct gatccttgac      300

gatatgccct gcatggacga tgcgaagctg cggcgcggac gccctaccat tcattctcat      360

tacggagagc atgtggcaat actggcggcg gttgccttgc tgagtaaagc ctttggcgta      420

attgccgatg cagatggcct cacgccgctg gcaaaaaatc gggcggtttc tgaactgtca      480

aacgccatcg gcatgcaagg attggttcag ggtcagttca aggatctgtc tgaaggggat      540

aagccgcgca gcgctgaagc tattttgatg acgaatcact ttaaaaccag cacgctgttt      600

tgtgcctcca tgcagatggc ctcgattgtt gcgaatgcct ccagcgaagc gcgtgattgc      660

ctgcatcgtt tttcacttga tcttggtcag gcatttcaac tgctggacga tttgaccgat      720

ggcatgaccg acaccggtaa ggatagcaat caggacgccg gtaaatcgac gctggtcaat      780

ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac atcttcagct tgccagtgag      840

catctctctg cggcctgcca acacgggcac gccactcaac attttattca ggcctggttt      900

gacaaaaaac tcgctgccgt cagttaataa tactagagct caaggaggta ctagatgaat      960

aatccgtcgt tactcaatca tgcggtcgaa acgatggcag ttggctcgaa aagttttgcg     1020

acagcctcaa agttatttga tgcaaaaacc cggcgcagcg tactgatgct ctacgcctgg     1080

tgccgccatt gtgacgatgt tattgacgat cagacgctgg gctttcaggc ccggcagcct     1140

gccttacaaa cgcccgaaca acgtctgatg caacttgaga tgaaaacgcg ccaggcctat     1200

gcaggatcgc agatgcacga accggcgttt gcggcttttc aggaagtggc tatggctcat     1260

gatatcgccc cggcttacgc gtttgatcat ctggaaggct tcgccatgga tgtacgcgaa     1320

gcgcaataca gccaactgga tgatacgctg cgctattgct atcacgttgc aggcgttgtc     1380

ggcttgatga tggcgcaaat catgggcgtg cgggataacg ccacgctgga ccgcgcctgt     1440

gaccttgggc tggcatttca gttgaccaat attgctcgcg atattgtgga cgatgcgcat     1500

gcgggccgct gttatctgcc ggcaagctgg ctggagcatg aaggtctgaa caaagagaat     1560

tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg cccgtcgttt ggtgcaggaa     1620

gcagaacctt actatttgtc tgccacagcc ggcctggcag ggttgcccct gcgttccgcc     1680

tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag gtgtcaaagt tgaacaggcc     1740

ggtcagcaag cctgggatca gcggcagtca acgaccacgc ccgaaaaatt aacgctgctg     1800

ctggccgcct ctggtcaggc ccttacttcc cggatgcggg ctcatcctcc ccgccctgcg     1860

catctctggc agcgcccgct ctaataatac tagagctcaa ggaggtacta gatgaaacca     1920

actacggtaa ttggtgcagg cttcggtggc ctggcactgg caattcgtct acaagctgcg     1980

gggatccccg tcttactgct tgaacaacgt gataaacccg gcggtcgggc ttatgtctac     2040

gaggatcagg ggtttacctt tgatgcaggc ccgacggtta tcaccgatcc cagtgccatt     2100

gaagaactgt ttgcactggc aggaaaacag ttaaaagagt atgtcgaact gctgccggtt     2160

acgccgtttt accgcctgtg ttgggagtca gggaaggtct ttaattacga taacgatcaa     2220

acccggctcg aagcgcagat tcagcagttt aatccccgcg atgtcgaagg ttatcgtcag     2280

tttctggact attcacgcgc ggtgtttaaa gaaggctatc taaagctcgg tactgtccct     2340

tttttatcgt tcagagacat gcttcgcgcc gcacctcaac tggcgaaact gcaagcatgg     2400

agaagcgttt acagtaaggt tgccagttac atcgaagatg aacatctgcg ccaggcgttt     2460

tctttccact cgctgttggt gggcggcaat cccttcgcca cctcatccat ttatacgttg     2520

atacacgcgc tggagcgtga gtggggcgtc tggtttccgc gtggcggcac cggcgcatta     2580

gttcagggga tgataaagct gtttcaggat ctgggtggcg aagtcgtgtt aaacgccaga     2640

gtcagccata tggaaacgac aggaaacaag attgaagccg tgcatttaga ggacggtcgc     2700

aggttcctga cgcaagccgt cgcgtcaaat gcagatgtgg ttcataccta tcgcgacctg     2760

ttaagccagc accctgccgc ggttaagcag tccaacaaac tgcaaactaa gcgcatgagt     2820

aactctctgt ttgtgctcta ttttggtttg aatcaccatc atgatcagct cgcgcatcac     2880

acggtttgtt tcggcccgcg ttaccgcgag ctgattgacg aaatttttaa tcatgatggc     2940

ctcgcagagg acttctcact ttatctgcac gcgccctgtg tcacggattc gtcactggcg     3000

cctgaaggtt gcggcagtta ctatgtgttg gcgccggtgc cgcatttagg caccgcgaac     3060

ctcgactgga cggttgaggg gccaaaacta cgcgaccgta tttttgcgta ccttgagcag     3120

cattacatgc ctggcttacg gagtcagctg gtcacgcacc ggatgtttac gccgtttgat     3180

tttcgcgacc agcttaatgc ctatcatggc tcagcctttt ctgtggagcc cgttcttacc     3240

cagagcgcct ggtttcggcc gcataaccgc gataaaacca ttactaatct ctacctggtc     3300

ggcgcaggca cgcatcccgg cgcaggcatt cctggcgtca tcggctcggc aaaagcgaca     3360

gcaggtttga tgctggagga tctgatataa taa                                  3393


<210>  4
<211>  137
<212>  DNA
<213>  artificial

<220>
<223>  a transcriptional terminator and assembly scar

<400>  4
tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt       60

tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg      120

gcctttctgc gtttata                                                     137


<210>  5
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  medium strength constitutive promoter and assembly scar.

<400>  5
tactagagtt gacagctagc tcagtcctag ggattgtgct agc                         43


<210>  6
<211>  452
<212>  DNA
<213>  artificial

<220>
<223>  strong ribosomal binding site, assembly scars, and coding 
       sequence for ZntR, a zinc responsive transcriptional activator 
       for PzntA

<400>  6
tactagagaa agaggagaaa tactagatgt atcgcattgg tgagctggca aaaatggcgg       60

aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc      120

gtactgaagg tgggtttcgc ctatataccg aaagcgatct ccagcgattg aaatttatcc      180

gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca      240

tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg      300

aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg      360

atgcctgttg tgggactgct catagcagtg tttattgttc gattcttgaa gctcttgaac      420

aaggggcgag tggcgttaag agtggttgtt ga                                    452


<210>  7
<211>  135
<212>  DNA
<213>  artificial

<220>
<223>  a transcriptional terminator and assembly scar

<400>  7
tactagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta       60

tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc      120

ctttctgcgt ttata                                                       135


<210>  8
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  a medium strength constitutive promoter and assembly scar

<400>  8
tactagagtt gacagctagc tcagtcctag ggattgtgct agc                         43


<210>  9
<211>  544
<212>  DNA
<213>  artificial

<220>
<223>  intermediate strength ribosomal binding site, assembly scars, and
       coding sequence for Zur, a zinc responsive transcriptional 
       repressor for PznuC

<400>  9
tactagagtc acacaggaaa cctactagat ggaaaagacc acaacgcagg agttattagc       60

gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg accccacagc gcctggaagt      120

gttgcgcctg atgagtctcc aagatggcgc tatcagcgct tatgatctgc ttgatttact      180

gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat cgcgcgctgg attttctgct      240

tgagcaaggt tttgtgcata aggtggaatc caccaacagt tatgtgctct gtcatctgtt      300

cgatcagccc acccatacgt cagccatgtt tatttgcgat cgctgcggcg cagtgaaaga      360

agagtgtgca gaaggcgtgg aagacattat gcatacgctg gcggcaaaaa tggggtttgc      420

cctgcggcat aatgtgattg aagcacatgg gctctgtgcg gcatgtgtag aagtggaagc      480

gtgtcgtcat cctgaacagt gccagcatga tcactctgtg caggtgaaaa agaaaccgcg      540

ttaa                                                                   544


<210>  10
<211>  137
<212>  DNA
<213>  artificial

<220>
<223>  a transcriptional terminator and assembly scar

<400>  10
tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt       60

tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg      120

gcctttctgc gtttata                                                     137


<210>  11
<211>  67
<212>  DNA
<213>  artificial

<220>
<223>  Pznta, a promoter sequence positively regulated by ZntR, and 
       assembly scar

<400>  11
tactagagct gtatctctga taaaacttga ctctggagtc gactccagag tgtatccttc       60

ggttaat                                                                 67


<210>  12
<211>  1211
<212>  DNA
<213>  artificial

<220>
<223>  a weak ribosomal binding site, coding sequence for carotenoid 
       gene to catalyze reaction of lycopene into beta carotene, and 
       assembly scars

<400>  12
tactagagtc acacaggact actagatgca accgcattat gatctgattc tcgtgggggc       60

tggactcgcg aatggcctta tcgccctgcg tcttcagcag cagcaacctg atatgcgtat      120

tttgcttatc gacgccgcac cccaggcggg cgggaatcat acgtggtcat ttcaccacga      180

tgatttgact gagagccaac atcgttggat agctccgctg gtggttcatc actggcccga      240

ctatcaggta cgctttccca cacgccgtcg taagctgaac agcggctact tttgtattac      300

ttctcagcgt ttcgctgagg ttttacagcg acagtttggc ccgcacttgt ggatggatac      360

cgcggtcgca gaggttaatg cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc      420

ccgcgcggtg attgacgggc ggggttatgc ggcaaattca gcactgagcg tgggcttcca      480

ggcgtttatt ggccaggaat ggcgattgag ccacccgcat ggtttatcgt ctcccattat      540

catggatgcc acggtcgatc agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc      600

gccgaccaga ttgttaattg aagacacgca ctatattgat aatgcgacat tagatcctga      660

atgcgcgcgg caaaatattt gcgactatgc cgcgcaacag ggttggcagc ttcagacact      720

gctgcgagaa gaacagggcg ccttacccat tactctgtcg ggcaatgccg acgcattctg      780

gcagcagcgc cccctggcct gtagtggatt acgtgccggt ctgttccatc ctaccaccgg      840

ctattcactg ccgctggcgg ttgccgtggc cgaccgcctg agtgcacttg atgtctttac      900

gtcggcctca attcaccatg ccattacgca ttttgcccgc gagcgctggc agcagcaggg      960

ctttttccgc atgctgaatc gcatgctgtt tttagccgga cccgccgatt cacgctggcg     1020

ggttatgcag cgtttttatg gtttacctga agatttaatt gcccgttttt atgcgggaaa     1080

actcacgctg accgatcggc tacgtattct gagcggcaag ccgcctgttc cggtattagc     1140

agcattgcaa gccattatga cgactcatcg tgctgctaac gacgaaaact acgctctggc     1200

tgcttaataa t                                                          1211


<210>  13
<211>  137
<212>  DNA
<213>  artificial

<220>
<223>  a transcriptional terminator and assembly scar.

<400>  13
tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt       60

tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg      120

gcctttctgc gtttata                                                     137


<210>  14
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  PznuC, a promoter sequence negatively regulated by Zur.

<400>  14
tactagagaa cataatgcga ccaataatcg taatgaatat gagaagtgtg atattataac       60

attt                                                                    64


<210>  15
<211>  7353
<212>  DNA
<213>  artificial

<220>
<223>  coding sequences, assembly scars, and ribosomal binding sites for
       vioABCDE for production of violacein

<400>  15
tactagagtt aaggaggtaa aaaaaatgaa acattcttcc gatatctgca ttgttggtgc       60

tggtatttct ggtttgacgt gcgcaagcca tctgctggac agcccggcat gccgtggtct      120

gagcctgcgt atctttgaca tgcagcaaga agccggtggc cgtatccgca gcaaaatgct      180

ggatggtaag gcaagcattg aactgggcgc aggtcgctac tcccctcagt tgcacccgca      240

tttccaaagc gcaatgcagc actatagcca aaagagcgaa gtctatccgt tcacccagtt      300

gaagttcaaa tctcacgtgc agcaaaagct gaagcgcgcc atgaatgaac tgtccccgcg      360

tctgaaagag catggtaaag agagcttttt gcagtttgtc agccgttatc aaggtcacga      420

tagcgcggtt ggtatgatcc gctctatggg ttacgacgca ctgttcctgc cggatatcag      480

cgcagaaatg gcctacgaca ttgtgggtaa gcacccggag atccagagcg tgacggacaa      540

cgacgcgaac caatggtttg cagcggaaac gggctttgct ggtctgattc agggcatcaa      600

ggctaaggtt aaggcggcag gtgcgcgttt tagcctgggt tatcgtctgc tgagcgtccg      660

taccgacggt gacggctacc tgctgcaact ggcaggtgac gacggctgga aactggagca      720

ccgtacccgc catctgattc tggcgattcc gccgagcgcg atggcgggtt tgaatgttga      780

ttttccagaa gcctggtccg gtgcgcgcta tggcagcctg ccgctgttta agggctttct      840

gacgtacggt gagccgtggt ggttggacta caaactggac gatcaggtgc tgattgttga      900

caacccgctg cgcaaaatct atttcaaagg cgataagtac ctgttcttct ataccgatag      960

cgagatggcg aattactggc gcggttgtgt cgcggagggc gaggacggtt acctggagca     1020

aattcgcacc catttggcta gcgcactggg tatcgtccgt gaacgtatcc cgcaaccgct     1080

ggcacacgtt cacaagtatt gggcgcacgg cgttgagttt tgccgtgatt ctgatattga     1140

ccacccgagc gcactgtctc atcgcgacag cggtatcatc gcgtgctccg atgcgtacac     1200

ggagcattgt ggttggatgg agggcggtct gctgagcgcc cgtgaggcaa gccgtctgct     1260

gttgcagcgt atcgccgcgt gattaaggag gtaaaaaaaa tgagcattct ggatttcccg     1320

cgtatccact tccgtggctg ggcccgtgtc aatgcgccga ccgcgaaccg cgatccgcac     1380

ggccacatcg atatggccag caataccgtg gcgatggcgg gtgagccgtt cgacctggca     1440

cgccatccta cggagttcca ccgtcacctg cgctccctgg gtccgcgctt cggcttggat     1500

ggtcgtgctg acccggaagg cccgttcagc ctggccgagg gctacaacgc tgccggtaac     1560

aaccactttt cgtgggagag cgcaaccgtt agccacgtgc aatgggatgg cggtgaggcg     1620

gatcgtggtg acggtctggt cggtgctcgt ttggcactgt ggggtcacta caatgattat     1680

ctgcgtacca ccttcaatcg tgctcgttgg gtcgacagcg acccgacgcg ccgtgacgct     1740

gcacaaatct atgcgggcca attcaccatt agcccggctg gtgccggtcc gggtacgccg     1800

tggctgttta cggcagacat tgatgatagc catggtgcac gttggacgcg tggcggccac     1860

attgcagagc gtggcggcca cttcttggat gaagagtttg gtctggcacg cctgtttcag     1920

ttctctgtgc cgaaagatca cccacatttt ctgtttcacc cgggtccgtt tgattccgag     1980

gcctggcgtc gtctgcaatt ggctctggag gatgacgacg ttctgggtct gaccgtgcaa     2040

tatgcgttgt tcaatatgag caccccgcct cagccgaaca gcccggtttt tcacgatatg     2100

gtcggtgttg tcggtctgtg gcgtcgtggt gaactggcga gctacccggc tggtcgtctg     2160

ctgcgtccgc gtcaaccggg tctgggtgac ctgaccctgc gcgtcaacgg tggtcgcgtt     2220

gcgctgaatt tggcgtgtgc cattccgttc agcactcgtg ccgcgcagcc aagcgcaccg     2280

gaccgcctga ccccggacct gggtgccaaa ctgccgctgg gcgatctgct gctgcgtgat     2340

gaggacggcg cactgttggc acgtgtgccg caggctctgt accaagacta ttggacgaat     2400

cacggtattg tggacctgcc gctgctgcgc gaaccgcgtg gtagcttgac cctgagcagc     2460

gaactggcgg agtggcgtga gcaagactgg gtcacccaaa gcgacgcgtc taacctgtac     2520

ctggaggcac cggatcgccg tcacggtcgc tttttccctg agagcatcgc gctgcgcagc     2580

tactttcgcg gtgaagcgcg tgcgcgtccg gatatcccgc atcgtatcga gggcatgggc     2640

ctggtcggcg tcgaatctcg tcaggatggc gacgctgcgg aatggcgtct gacgggtctg     2700

cgtccgggtc cggcacgcat tgttctggac gatggtgccg aggcgatccc tctgcgtgtt     2760

ctgcctgacg attgggcgct ggatgacgcg accgtcgaag aagtggatta cgcctttttg     2820

taccgccacg ttatggcgta ttacgagctg gtgtatccat tcatgagcga caaggtgttt     2880

tccctggctg atcgttgcaa atgtgaaacg tacgcacgtc tgatgtggca gatgtgtgat     2940

ccgcagaacc gcaacaagtc ctattacatg ccgagcaccc gcgaactgtc ggcaccgaaa     3000

gctcgtttgt tcttgaagta tctggcccac gtggaaggcc aggcacgcct gcaagcacct     3060

ccgccagcgg gtccggcacg cattgaatct aaagcccagt tggcggcaga gctgcgtaaa     3120

gccgtcgacc tggagctgtc tgtgatgctg caatacctgt acgcggcgta tagcattccg     3180

aactatgcac agggccaaca acgtgttcgt gacggtgcgt ggaccgccga gcagctgcaa     3240

ctggcgtgcg gtagcggtga ccgtcgccgt gatggcggta ttcgtgcagc actgctggaa     3300

attgctcatg aagaaatgat tcattacctg gtcgttaaca acctgctgat ggccctgggc     3360

gagccgttct acgcgggtgt cccgctgatg ggcgaagcgg cacgtcaggc gtttggcctg     3420

gacaccgagt tcgctctgga accgtttagc gaaagcacgc tggcacgttt tgttcgtctg     3480

gaatggccgc actttatccc agcaccgggc aaatccatcg cggactgcta tgccgccatt     3540

cgtcaggcgt ttttggatct gccggacttg tttggtggcg aggcaggtaa gcgtggcggt     3600

gaacaccacc tgttcctgaa tgagctgacc aaccgtgcgc atccgggtta tcaactggaa     3660

gttttcgatc gcgactcggc gctgtttggt attgcatttg tgaccgatca gggcgaaggt     3720

ggcgctctgg acagcccgca ctacgaacat agccattttc aacgtctgcg tgaaatgagc     3780

gcgcgtatca tggctcaaag cgcaccgttc gaaccggcgc tgccggcgtt gcgtaatccg     3840

gttctggatg agagcccggg ttgccaacgt gtcgcagacg gtcgtgcgcg tgcgctgatg     3900

gcattgtacc aaggcgttta tgagctgatg tttgcgatga tggcgcagca cttcgccgtg     3960

aaaccgctgg gtagcttgcg tcgcagccgc ctgatgaacg cagcaatcga tctgatgacc     4020

ggtctgttgc gtccgctgag ctgcgcgctg atgaacctgc caagcggcat cgccggtcgc     4080

acggccggtc cgccgctgcc gggtccggtt gacacccgta gctatgacga ctacgcgctg     4140

ggctgtcgca tgctggcacg ccgttgcgag cgtctgctgg agcaggcgag catgctggaa     4200

ccgggttggc tgccggatgc gcagatggag ctgctggatt tctatcgtcg ccaaatgctg     4260

gacttggcgt gcggcaaact gagccgcgag gcctaaggat ccttaaggag gtaaaaaaaa     4320

tgaaacgtgc gattatcgtt ggtggcggcc tggcgggtgg cctgaccgcg atctacctgg     4380

cgaagcgtgg ctacgaagtg cacgtcgtgg agaagcgtgg tgatcctctg cgcgatctga     4440

gctcttacgt ggacgttgtt agcagccgtg cgatcggcgt gagcatgacc gttcgtggta     4500

tcaagagcgt tttggctgcg ggcattccgc gtgcagagct ggatgcgtgt ggcgaaccga     4560

tcgtggcaat ggctttctcc gtgggtggtc agtatcgcat gcgcgaactg aagccgttgg     4620

aggatttccg tccgctgagc ttgaaccgtg cggcgtttca aaagctgctg aacaaatacg     4680

cgaacctggc aggcgttcgt tactactttg agcataagtg cctggatgtt gacctggatg     4740

gtaagagcgt gttgattcag ggcaaagatg gtcagccgca gcgtctgcaa ggtgacatga     4800

ttatcggtgc ggatggcgcc cacagcgccg tccgtcaggc gatgcagagc ggcctgcgtc     4860

gtttcgagtt ccagcaaacg ttcttccgcc atggctacaa aaccctggtt ttgccggacg     4920

cgcaagcact gggttaccgt aaagacacgc tgtacttttt cggcatggat tccggtggcc     4980

tgttcgcggg tcgtgcggct acgatcccag atggtagcgt cagcatcgcc gtttgcctgc     5040

cgtactcggg tagcccttcc ctgacgacca ccgacgaacc gacgatgcgt gcgttcttcg     5100

atcgttactt cggtggcctg ccgcgtgacg cgcgtgacga aatgctgcgt cagtttctgg     5160

cgaagccgag caacgacctg attaacgtgc gctctagcac ctttcactat aagggtaatg     5220

tgctgttgct gggtgatgct gcgcatgcga ctgcgccgtt cctgggtcag ggtatgaaca     5280

tggcgctgga ggacgcccgc acgtttgtcg agctgctgga ccgccaccag ggcgaccaag     5340

acaaagcctt tccggagttc acggagctgc gcaaagtcca ggcagacgca atgcaagaca     5400

tggctcgcgc caactatgac gttttgagct gctcgaaccc gatctttttc atgcgtgcgc     5460

gttacacgcg ttacatgcat tccaagtttc cgggcctgta tccgccggat atggccgaga     5520

aactgtactt tacgagcgag ccgtacgatc gtctgcaaca aatccagcgt aaacagaatg     5580

tttggtacaa gattggtcgc gtgaattgaa gatctttaag gaggtaaaaa aaatgaagat     5640

tctggtcatt ggtgctggtc cagctggtct ggttttcgca tcccaactga agcaggcacg     5700

ccctttgtgg gccattgaca tcgtggagaa gaatgacgag caagaagtgc tgggctgggg     5760

tgtcgtgctg cctggccgtc cgggtcagca cccggcgaac ccgctgtcct atctggatgc     5820

accggagcgt ctgaatccgc aatttctgga ggacttcaaa ctggtgcatc ataatgagcc     5880

gtccttgatg tccacgggcg ttttgttgtg cggcgtggag cgtcgcggtc tggttcacgc     5940

gctgcgcgat aagtgccgca gccaaggcat tgctattcgt ttcgaaagcc cgttgctgga     6000

acacggtgag ctgccgctgg cggactatga tctggtggtc ctggctaatg gtgttaatca     6060

caaaaccgcg catttcaccg aggctctggt cccgcaggtg gactacggcc gcaataagta     6120

catttggtat ggcactagcc agctgttcga tcagatgaat ctggtttttc gtacccatgg     6180

taaagatatc tttatcgcgc atgcctataa gtatagcgat accatgagca cgttcattgt     6240

cgaatgtagc gaagagactt acgcacgcgc acgcctgggc gaaatgtccg aagaggcgag     6300

cgcagaatac gttgctaagg tgttccaggc cgagctgggt ggtcacggcc tggtgagcca     6360

gccgggtctg ggttggcgta acttcatgac gttgtctcat gaccgttgtc atgatggtaa     6420

gttggttctg ctgggtgacg cgctgcaaag cggtcacttt agcatcggcc acggcaccac     6480

gatggccgtg gtggtggcgc agctgctggt taaagcgctg tgtaccgaag atggtgtgcc     6540

tgccgcgctg aaacgtttcg aagagcgtgc cctgccgctg gtgcagttgt tccgtggcca     6600

cgcagacaac agccgcgttt ggttcgaaac cgtcgaagag cgcatgcacc tgtcctcggc     6660

ggaatttgtg caaagcttcg acgcacgccg caaaagcctg ccgccgatgc cggaagcact     6720

ggcgcagaat ctgcgttatg ctttgcagcg ctgatgatca ttaaggaggt aaaaaaaatg     6780

gagaaccgtg agccaccact gttgccagcc cgttggagca gcgcctatgt ctcttattgg     6840

agcccgatgc tgccggatga ccagctgacc agcggctatt gctggttcga ctatgaacgt     6900

gacatctgtc gtattgacgg cctgttcaat ccgtggagcg agcgtgatac tggttatcgc     6960

ctgtggatgt cggaggttgg taatgcggcc agcggccgta cctggaaaca aaaagtcgcc     7020

tatggtcgtg agcgtaccgc cctgggtgaa cagctgtgtg agcgtccgct ggatgatgag     7080

actggccctt ttgccgaatt gttcctgcca cgcgatgtcc tgcgccgtct gggtgcccgt     7140

cacattggcc gtcgcgtggt tctgggtcgc gaagcggacg gttggcgtta ccagcgccca     7200

ggtaaaggtc cgagcaccct gtacctggat gcggcgagcg gcactccact gcgcatggtc     7260

accggcgatg aagcgtcgcg tgcaagcctg cgtgattttc cgaatgtgag cgaggcggag     7320

atcccggacg cggttttcgc ggccaagcgc taa                                  7353


<210>  16
<211>  3252
<212>  DNA
<213>  artificial

<220>
<223>  pSB3T5, a medium copy vector

<400>  16
tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc       60

aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca      120

ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta      180

gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc      240

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta      300

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg      360

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga      420

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac      480

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa      540

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt      600

gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg      660

gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag      720

gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga      780

ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc      840

gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt      900

tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga      960

atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg     1020

agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt     1080

cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg     1140

aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca     1200

ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta     1260

gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg     1320

gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac     1380

atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct     1440

gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg     1500

atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa     1560

cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt     1620

attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat     1680

atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac     1740

tagaggagat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc     1800

acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt     1860

cattctcggc accgtcaccc tggacgctgt aggcataggc ttggttatgc cggtactgcc     1920

gggcctcttg cgggatatcg tccattccga cagtattgcc agtcactatg gcgtgctgct     1980

tgcgctctat gcgttgatgc aatttctttg cgcacccgtt ctcggagccc tgtccgaccg     2040

ctttggccgc cgtccagtcc tgctcgcttc gctccttgga gccactatcg actacgcgat     2100

catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg cgggcatcac     2160

gggtgccaca ggtgcggttg ctggtgccta tatcgccgac atcaccgatg gggaagatcg     2220

ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt     2280

ggccggggga ctgttgggtg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct     2340

caacggcctc aacctcctcc tgggctgctt ccttatgcag gaatcgcata agggagagcg     2400

ccgtccgatg cccttgcgtg ccttcaatcc agtcagctcc ttccggtggg cgcggggcat     2460

gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag gacaggttcc     2520

ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg     2580

cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcacgggccc     2640

cgccaccaaa cgtttcggcg agaagcaggc cattatcgcg ggcatggcgg ccgacgcgct     2700

gggctacgtc ttgctggcgt tcgcgacgcg cggctggatg gccttcccca ttatgattct     2760

tctcgcttcc ggcggcatcg gtatgcccgc gttgcaggcc atgctgtccc gccaagtaga     2820

tgacgaccat cagggacagc ttcaagggtc gctcgcggct cttaccagcc tcacttcgat     2880

cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt     2940

ggcatggatt gtaggtgccg ccctttacct tgtctgcctc cccgcgttgc gtcgcggtgc     3000

atggagccgg gccacctcga cctaataata ctagctccgg caaaaaaacg ggcaaggtgt     3060

caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt     3120

ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg     3180

agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaattcgcgg     3240

ccgcttctag ag                                                         3252


<210>  17
<211>  10864
<212>  DNA
<213>  artificial

<220>
<223>  an engineered T7 system to control lycopene expression

<400>  17
tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc       60

aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca      120

ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta      180

gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc      240

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta      300

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg      360

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga      420

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac      480

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa      540

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt      600

gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg      660

gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag      720

gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga      780

ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc      840

gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt      900

tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga      960

atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg     1020

agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt     1080

cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg     1140

aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca     1200

ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta     1260

gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg     1320

gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac     1380

atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct     1440

gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg     1500

atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa     1560

cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt     1620

attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat     1680

atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac     1740

tagaggagat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc     1800

acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt     1860

cattctcggc accgtcaccc tggacgctgt aggcataggc ttggttatgc cggtactgcc     1920

gggcctcttg cgggatatcg tccattccga cagtattgcc agtcactatg gcgtgctgct     1980

tgcgctctat gcgttgatgc aatttctttg cgcacccgtt ctcggagccc tgtccgaccg     2040

ctttggccgc cgtccagtcc tgctcgcttc gctccttgga gccactatcg actacgcgat     2100

catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg cgggcatcac     2160

gggtgccaca ggtgcggttg ctggtgccta tatcgccgac atcaccgatg gggaagatcg     2220

ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt     2280

ggccggggga ctgttgggtg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct     2340

caacggcctc aacctcctcc tgggctgctt ccttatgcag gaatcgcata agggagagcg     2400

ccgtccgatg cccttgcgtg ccttcaatcc agtcagctcc ttccggtggg cgcggggcat     2460

gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag gacaggttcc     2520

ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg     2580

cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcacgggccc     2640

cgccaccaaa cgtttcggcg agaagcaggc cattatcgcg ggcatggcgg ccgacgcgct     2700

gggctacgtc ttgctggcgt tcgcgacgcg cggctggatg gccttcccca ttatgattct     2760

tctcgcttcc ggcggcatcg gtatgcccgc gttgcaggcc atgctgtccc gccaagtaga     2820

tgacgaccat cagggacagc ttcaagggtc gctcgcggct cttaccagcc tcacttcgat     2880

cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt     2940

ggcatggatt gtaggtgccg ccctttacct tgtctgcctc cccgcgttgc gtcgcggtgc     3000

atggagccgg gccacctcga cctaataata ctagctccgg caaaaaaacg ggcaaggtgt     3060

caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt     3120

ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg     3180

agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaattcgcgg     3240

ccgcttctag agtaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg     3300

ctactagtca cacaggacta ctagatgaaa ccagtaacgt tatacgatgt cgcagagtat     3360

gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg     3420

aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg     3480

gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc     3540

ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc     3600

agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac     3660

aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat     3720

gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac     3780

cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag     3840

catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc     3900

tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg     3960

atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg     4020

ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc     4080

gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga     4140

tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat     4200

tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg     4260

gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc     4320

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     4380

gtttcccgat actagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct     4440

ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt     4500

cgggtgggcc tttctgcgtt tatatactag aattgtgagc ggataacaac tgatggtgtg     4560

agcggataac aagattatga gcacatacta gtcacacagg actactagat gaacacgatt     4620

aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg     4680

gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac     4740

gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt     4800

gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc     4860

atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag     4920

ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct     4980

tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc     5040

attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa     5100

aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa     5160

gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg     5220

cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc     5280

ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc     5340

gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc     5400

tctccgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt     5460

ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca     5520

ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa     5580

aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg     5640

aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg     5700

gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg     5760

taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa     5820

gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt     5880

cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt     5940

acgctggcga aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt     6000

gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa     6060

aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag     6120

caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac     6180

ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag     6240

cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt     6300

gaaaccgttc aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca     6360

gacgcaatca atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa     6420

atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt     6480

gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc     6540

ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt     6600

ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct     6660

gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag     6720

ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct     6780

gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag     6840

acgcgcttga acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac     6900

aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac     6960

agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc     7020

gaatcttttg cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg     7080

ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat     7140

ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt     7200

ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa     7260

taatactagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt     7320

ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg     7380

ggcctttctg cgtttatata ctagtaatac gactcactat aggaattgtg agcggataac     7440

aagattataa ttgtgagcgc tcacaattta ctagtcacac aggaaagtac tagatgacgg     7500

tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta ctggctgata     7560

ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt gtgggtgccg     7620

cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg ctgttgctga     7680

ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg gcctgtgcgg     7740

tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg gacgatgcga     7800

agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg gcaatactgg     7860

cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat ggcctcacgc     7920

cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg caaggattgg     7980

ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct gaagctattt     8040

tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctccatgcag atggcctcga     8100

ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca cttgatcttg     8160

gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc ggtaaggata     8220

gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg gcggttgaag     8280

aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc tgccaacacg     8340

ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct gccgtcagtt     8400

aatactagtc acacaggaaa gtactagatg aataatccgt cgttactcaa tcatgcggtc     8460

gaaacgatgg cagttggctc gaaaagtttt gcgacagcct caaagttatt tgatgcaaaa     8520

acccggcgca gcgtactgat gctctacgcc tggtgccgcc attgtgacga tgttattgac     8580

gatcagacgc tgggctttca ggcccggcag cctgccttac aaacgcccga acaacgtctg     8640

atgcaacttg agatgaaaac gcgccaggcc tatgcaggat cgcagatgca cgaaccggcg     8700

tttgcggctt ttcaggaagt ggctatggct catgatatcg ccccggctta cgcgtttgat     8760

catctggaag gcttcgccat ggatgtacgc gaagcgcaat acagccaact ggatgatacg     8820

ctgcgctatt gctatcacgt tgcaggcgtt gtcggcttga tgatggcgca aatcatgggc     8880

gtgcgggata acgccacgct ggaccgcgcc tgtgaccttg ggctggcatt tcagttgacc     8940

aatattgctc gcgatattgt ggacgatgcg catgcgggcc gctgttatct gccggcaagc     9000

tggctggagc atgaaggtct gaacaaagag aattatgcgg cacctgaaaa ccgtcaggcg     9060

ctgagccgta tcgcccgtcg tttggtgcag gaagcagaac cttactattt gtctgccaca     9120

gccggcctgg cagggttgcc cctgcgttcc gcctgggcaa tcgctacggc gaagcaggtt     9180

taccggaaaa taggtgtcaa agttgaacag gccggtcagc aagcctggga tcagcggcag     9240

tcaacgacca cgcccgaaaa attaacgctg ctgctggccg cctctggtca ggcccttact     9300

tcccggatgc gggctcatcc tccccgccct gcgcatctct ggcagcgccc gctctaatac     9360

tagtcacaca ggaaagtact agatgaaacc aactacggta attggtgcag gcttcggtgg     9420

cctggcactg gcaattcgtc tacaagctgc ggggatcccc gtcttactgc ttgaacaacg     9480

tgataaaccc ggcggtcggg cttatgtcta cgaggatcag gggtttacct ttgatgcagg     9540

cccgacggtt atcaccgatc ccagtgccat tgaagaactg tttgcactgg caggaaaaca     9600

gttaaaagag tatgtcgaac tgctgccggt tacgccgttt taccgcctgt gttgggagtc     9660

agggaaggtc tttaattacg ataacgatca aacccggctc gaagcgcaga ttcagcagtt     9720

taatccccgc gatgtcgaag gttatcgtca gtttctggac tattcacgcg cggtgtttaa     9780

agaaggctat ctaaagctcg gtactgtccc ttttttatcg ttcagagaca tgcttcgcgc     9840

cgcacctcaa ctggcgaaac tgcaagcatg gagaagcgtt tacagtaagg ttgccagtta     9900

catcgaagat gaacatctgc gccaggcgtt ttctttccac tcgctgttgg tgggcggcaa     9960

tcccttcgcc acctcatcca tttatacgtt gatacacgcg ctggagcgtg agtggggcgt    10020

ctggtttccg cgtggcggca ccggcgcatt agttcagggg atgataaagc tgtttcagga    10080

tctgggtggc gaagtcgtgt taaacgccag agtcagccat atggaaacga caggaaacaa    10140

gattgaagcc gtgcatttag aggacggtcg caggttcctg acgcaagccg tcgcgtcaaa    10200

tgcagatgtg gttcatacct atcgcgacct gttaagccag caccctgccg cggttaagca    10260

gtccaacaaa ctgcaaacta agcgcatgag taactctctg tttgtgctct attttggttt    10320

gaatcaccat catgatcagc tcgcgcatca cacggtttgt ttcggcccgc gttaccgcga    10380

gctgattgac gaaattttta atcatgatgg cctcgcagag gacttctcac tttatctgca    10440

cgcgccctgt gtcacggatt cgtcactggc gcctgaaggt tgcggcagtt actatgtgtt    10500

ggcgccggtg ccgcatttag gcaccgcgaa cctcgactgg acggttgagg ggccaaaact    10560

acgcgaccgt atttttgcgt accttgagca gcattacatg cctggcttac ggagtcagct    10620

ggtcacgcac cggatgttta cgccgtttga ttttcgcgac cagcttaatg cctatcatgg    10680

ctcagccttt tctgtggagc ccgttcttac ccagagcgcc tggtttcggc cgcataaccg    10740

cgataaaacc attactaatc tctacctggt cggcgcaggc acgcatcccg gcgcaggcat    10800

tcctggcgtc atcggctcgg caaaagcgac agcaggtttg atgctggagg atctgatata    10860

ataa                                                                 10864


<210>  18
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  strong degradation tag

<400>  18

Ala Ala Asn Asp Glu Asn Tyr Ala Leu Ala Ala 
1               5                   10      


<210>  19
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  weak degradation tag

<400>  19

Ala Ala Asn Asp Glu Asn Tyr Ala Asp Ala Ser 
1               5                   10      


<210>  20
<211>  17139
<212>  DNA
<213>  artificial

<220>
<223>  construct for detecting levels of zinc

<400>  20
ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt       60

gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct      120

gcgtttatat actagagctg atggctagct cagtcctagg gattatgcta gctactagag      180

gaggtactag atgacggtct gcgcaaaaaa acacgttcat ctcactcgcg atgctgcgga      240

gcagttactg gctgatattg atcgacgcct tgatcagtta ttgcccgtgg agggagaacg      300

ggatgttgtg ggtgccgcga tgcgtgaagg tgcgctggca ccgggaaaac gtattcgccc      360

catgttgctg ttgctgaccg cccgcgatct gggttgcgct gtcagccatg acggattact      420

ggatttggcc tgtgcggtgg aaatggtcca cgcggcttcg ctgatccttg acgatatgcc      480

ctgcatggac gatgcgaagc tgcggcgcgg acgccctacc attcattctc attacggaga      540

gcatgtggca atactggcgg cggttgcctt gctgagtaaa gcctttggcg taattgccga      600

tgcagatggc ctcacgccgc tggcaaaaaa tcgggcggtt tctgaactgt caaacgccat      660

cggcatgcaa ggattggttc agggtcagtt caaggatctg tctgaagggg ataagccgcg      720

cagcgctgaa gctattttga tgacgaatca ctttaaaacc agcacgctgt tttgtgcctc      780

catgcagatg gcctcgattg ttgcgaatgc ctccagcgaa gcgcgtgatt gcctgcatcg      840

tttttcactt gatcttggtc aggcatttca actgctggac gatttgaccg atggcatgac      900

cgacaccggt aaggatagca atcaggacgc cggtaaatcg acgctggtca atctgttagg      960

cccgagggcg gttgaagaac gtctgagaca acatcttcag cttgccagtg agcatctctc     1020

tgcggcctgc caacacgggc acgccactca acattttatt caggcctggt ttgacaaaaa     1080

actcgctgcc gtcagttaat aatactagag ctcaaggagg tactagatga ataatccgtc     1140

gttactcaat catgcggtcg aaacgatggc agttggctcg aaaagttttg cgacagcctc     1200

aaagttattt gatgcaaaaa cccggcgcag cgtactgatg ctctacgcct ggtgccgcca     1260

ttgtgacgat gttattgacg atcagacgct gggctttcag gcccggcagc ctgccttaca     1320

aacgcccgaa caacgtctga tgcaacttga gatgaaaacg cgccaggcct atgcaggatc     1380

gcagatgcac gaaccggcgt ttgcggcttt tcaggaagtg gctatggctc atgatatcgc     1440

cccggcttac gcgtttgatc atctggaagg cttcgccatg gatgtacgcg aagcgcaata     1500

cagccaactg gatgatacgc tgcgctattg ctatcacgtt gcaggcgttg tcggcttgat     1560

gatggcgcaa atcatgggcg tgcgggataa cgccacgctg gaccgcgcct gtgaccttgg     1620

gctggcattt cagttgacca atattgctcg cgatattgtg gacgatgcgc atgcgggccg     1680

ctgttatctg ccggcaagct ggctggagca tgaaggtctg aacaaagaga attatgcggc     1740

acctgaaaac cgtcaggcgc tgagccgtat cgcccgtcgt ttggtgcagg aagcagaacc     1800

ttactatttg tctgccacag ccggcctggc agggttgccc ctgcgttccg cctgggcaat     1860

cgctacggcg aagcaggttt accggaaaat aggtgtcaaa gttgaacagg ccggtcagca     1920

agcctgggat cagcggcagt caacgaccac gcccgaaaaa ttaacgctgc tgctggccgc     1980

ctctggtcag gcccttactt cccggatgcg ggctcatcct ccccgccctg cgcatctctg     2040

gcagcgcccg ctctaataat actagagctc aaggaggtac tagatgaaac caactacggt     2100

aattggtgca ggcttcggtg gcctggcact ggcaattcgt ctacaagctg cggggatccc     2160

cgtcttactg cttgaacaac gtgataaacc cggcggtcgg gcttatgtct acgaggatca     2220

ggggtttacc tttgatgcag gcccgacggt tatcaccgat cccagtgcca ttgaagaact     2280

gtttgcactg gcaggaaaac agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt     2340

ttaccgcctg tgttgggagt cagggaaggt ctttaattac gataacgatc aaacccggct     2400

cgaagcgcag attcagcagt ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga     2460

ctattcacgc gcggtgttta aagaaggcta tctaaagctc ggtactgtcc cttttttatc     2520

gttcagagac atgcttcgcg ccgcacctca actggcgaaa ctgcaagcat ggagaagcgt     2580

ttacagtaag gttgccagtt acatcgaaga tgaacatctg cgccaggcgt tttctttcca     2640

ctcgctgttg gtgggcggca atcccttcgc cacctcatcc atttatacgt tgatacacgc     2700

gctggagcgt gagtggggcg tctggtttcc gcgtggcggc accggcgcat tagttcaggg     2760

gatgataaag ctgtttcagg atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca     2820

tatggaaacg acaggaaaca agattgaagc cgtgcattta gaggacggtc gcaggttcct     2880

gacgcaagcc gtcgcgtcaa atgcagatgt ggttcatacc tatcgcgacc tgttaagcca     2940

gcaccctgcc gcggttaagc agtccaacaa actgcaaact aagcgcatga gtaactctct     3000

gtttgtgctc tattttggtt tgaatcacca tcatgatcag ctcgcgcatc acacggtttg     3060

tttcggcccg cgttaccgcg agctgattga cgaaattttt aatcatgatg gcctcgcaga     3120

ggacttctca ctttatctgc acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg     3180

ttgcggcagt tactatgtgt tggcgccggt gccgcattta ggcaccgcga acctcgactg     3240

gacggttgag gggccaaaac tacgcgaccg tatttttgcg taccttgagc agcattacat     3300

gcctggctta cggagtcagc tggtcacgca ccggatgttt acgccgtttg attttcgcga     3360

ccagcttaat gcctatcatg gctcagcctt ttctgtggag cccgttctta cccagagcgc     3420

ctggtttcgg ccgcataacc gcgataaaac cattactaat ctctacctgg tcggcgcagg     3480

cacgcatccc ggcgcaggca ttcctggcgt catcggctcg gcaaaagcga cagcaggttt     3540

gatgctggag gatctgatat aataatacta gagccaggca tcaaataaaa cgaaaggctc     3600

agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga     3660

gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacagcta     3720

gctcagtcct agggattgtg ctagctacta gagaaagagg agaaatacta gatgtatcgc     3780

attggtgagc tggcaaaaat ggcggaagta acacccgaca cgattcgtta ttacgaaaaa     3840

cagcagatga tggagcatga agtgcgtact gaaggtgggt ttcgcctata taccgaaagc     3900

gatctccagc gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg     3960

atccgcgagt tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa     4020

ggcattgtgc aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg     4080

cagcgttcct tgcaacgcct taacgatgcc tgttgtggga ctgctcatag cagtgtttat     4140

tgttcgattc ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ttgttgatac     4200

tagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct     4260

gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt     4320

tctgcgttta tatactagag ttgacagcta gctcagtcct agggattgtg ctagctacta     4380

gagtcacaca ggaaacctac tagatggaaa agaccacaac gcaggagtta ttagcgcagg     4440

ctgaaaaaat ctgcgcgcag cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc     4500

gcctgatgag tctccaagat ggcgctatca gcgcttatga tctgcttgat ttactgcgcg     4560

aagctgaacc gcaagccaag ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc     4620

aaggttttgt gcataaggtg gaatccacca acagttatgt gctctgtcat ctgttcgatc     4680

agcccaccca tacgtcagcc atgtttattt gcgatcgctg cggcgcagtg aaagaagagt     4740

gtgcagaagg cgtggaagac attatgcata cgctggcggc aaaaatgggg tttgccctgc     4800

ggcataatgt gattgaagca catgggctct gtgcggcatg tgtagaagtg gaagcgtgtc     4860

gtcatcctga acagtgccag catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaat     4920

actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt     4980

atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg     5040

cctttctgcg tttatatact agagctgtat ctctgataaa acttgactct ggagtcgact     5100

ccagagtgta tccttcggtt aattactaga gtcacacagg actactagat gcaaccgcat     5160

tatgatctga ttctcgtggg ggctggactc gcgaatggcc ttatcgccct gcgtcttcag     5220

cagcagcaac ctgatatgcg tattttgctt atcgacgccg caccccaggc gggcgggaat     5280

catacgtggt catttcacca cgatgatttg actgagagcc aacatcgttg gatagctccg     5340

ctggtggttc atcactggcc cgactatcag gtacgctttc ccacacgccg tcgtaagctg     5400

aacagcggct acttttgtat tacttctcag cgtttcgctg aggttttaca gcgacagttt     5460

ggcccgcact tgtggatgga taccgcggtc gcagaggtta atgcggaatc tgttcggttg     5520

aaaaagggtc aggttatcgg tgcccgcgcg gtgattgacg ggcggggtta tgcggcaaat     5580

tcagcactga gcgtgggctt ccaggcgttt attggccagg aatggcgatt gagccacccg     5640

catggtttat cgtctcccat tatcatggat gccacggtcg atcagcaaaa tggttatcgc     5700

ttcgtgtaca gcctgccgct ctcgccgacc agattgttaa ttgaagacac gcactatatt     5760

gataatgcga cattagatcc tgaatgcgcg cggcaaaata tttgcgacta tgccgcgcaa     5820

cagggttggc agcttcagac actgctgcga gaagaacagg gcgccttacc cattactctg     5880

tcgggcaatg ccgacgcatt ctggcagcag cgccccctgg cctgtagtgg attacgtgcc     5940

ggtctgttcc atcctaccac cggctattca ctgccgctgg cggttgccgt ggccgaccgc     6000

ctgagtgcac ttgatgtctt tacgtcggcc tcaattcacc atgccattac gcattttgcc     6060

cgcgagcgct ggcagcagca gggctttttc cgcatgctga atcgcatgct gtttttagcc     6120

ggacccgccg attcacgctg gcgggttatg cagcgttttt atggtttacc tgaagattta     6180

attgcccgtt tttatgcggg aaaactcacg ctgaccgatc ggctacgtat tctgagcggc     6240

aagccgcctg ttccggtatt agcagcattg caagccatta tgacgactca tcgtgctgct     6300

aacgacgaaa actacgctct ggctgcttaa taatactaga gccaggcatc aaataaaacg     6360

aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct     6420

ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa     6480

cataatgcga ccaataatcg taatgaatat gagaagtgtg atattataac attttactag     6540

agttaaggag gtaaaaaaaa tgaaacattc ttccgatatc tgcattgttg gtgctggtat     6600

ttctggtttg acgtgcgcaa gccatctgct ggacagcccg gcatgccgtg gtctgagcct     6660

gcgtatcttt gacatgcagc aagaagccgg tggccgtatc cgcagcaaaa tgctggatgg     6720

taaggcaagc attgaactgg gcgcaggtcg ctactcccct cagttgcacc cgcatttcca     6780

aagcgcaatg cagcactata gccaaaagag cgaagtctat ccgttcaccc agttgaagtt     6840

caaatctcac gtgcagcaaa agctgaagcg cgccatgaat gaactgtccc cgcgtctgaa     6900

agagcatggt aaagagagct ttttgcagtt tgtcagccgt tatcaaggtc acgatagcgc     6960

ggttggtatg atccgctcta tgggttacga cgcactgttc ctgccggata tcagcgcaga     7020

aatggcctac gacattgtgg gtaagcaccc ggagatccag agcgtgacgg acaacgacgc     7080

gaaccaatgg tttgcagcgg aaacgggctt tgctggtctg attcagggca tcaaggctaa     7140

ggttaaggcg gcaggtgcgc gttttagcct gggttatcgt ctgctgagcg tccgtaccga     7200

cggtgacggc tacctgctgc aactggcagg tgacgacggc tggaaactgg agcaccgtac     7260

ccgccatctg attctggcga ttccgccgag cgcgatggcg ggtttgaatg ttgattttcc     7320

agaagcctgg tccggtgcgc gctatggcag cctgccgctg tttaagggct ttctgacgta     7380

cggtgagccg tggtggttgg actacaaact ggacgatcag gtgctgattg ttgacaaccc     7440

gctgcgcaaa atctatttca aaggcgataa gtacctgttc ttctataccg atagcgagat     7500

ggcgaattac tggcgcggtt gtgtcgcgga gggcgaggac ggttacctgg agcaaattcg     7560

cacccatttg gctagcgcac tgggtatcgt ccgtgaacgt atcccgcaac cgctggcaca     7620

cgttcacaag tattgggcgc acggcgttga gttttgccgt gattctgata ttgaccaccc     7680

gagcgcactg tctcatcgcg acagcggtat catcgcgtgc tccgatgcgt acacggagca     7740

ttgtggttgg atggagggcg gtctgctgag cgcccgtgag gcaagccgtc tgctgttgca     7800

gcgtatcgcc gcgtgattaa ggaggtaaaa aaaatgagca ttctggattt cccgcgtatc     7860

cacttccgtg gctgggcccg tgtcaatgcg ccgaccgcga accgcgatcc gcacggccac     7920

atcgatatgg ccagcaatac cgtggcgatg gcgggtgagc cgttcgacct ggcacgccat     7980

cctacggagt tccaccgtca cctgcgctcc ctgggtccgc gcttcggctt ggatggtcgt     8040

gctgacccgg aaggcccgtt cagcctggcc gagggctaca acgctgccgg taacaaccac     8100

ttttcgtggg agagcgcaac cgttagccac gtgcaatggg atggcggtga ggcggatcgt     8160

ggtgacggtc tggtcggtgc tcgtttggca ctgtggggtc actacaatga ttatctgcgt     8220

accaccttca atcgtgctcg ttgggtcgac agcgacccga cgcgccgtga cgctgcacaa     8280

atctatgcgg gccaattcac cattagcccg gctggtgccg gtccgggtac gccgtggctg     8340

tttacggcag acattgatga tagccatggt gcacgttgga cgcgtggcgg ccacattgca     8400

gagcgtggcg gccacttctt ggatgaagag tttggtctgg cacgcctgtt tcagttctct     8460

gtgccgaaag atcacccaca ttttctgttt cacccgggtc cgtttgattc cgaggcctgg     8520

cgtcgtctgc aattggctct ggaggatgac gacgttctgg gtctgaccgt gcaatatgcg     8580

ttgttcaata tgagcacccc gcctcagccg aacagcccgg tttttcacga tatggtcggt     8640

gttgtcggtc tgtggcgtcg tggtgaactg gcgagctacc cggctggtcg tctgctgcgt     8700

ccgcgtcaac cgggtctggg tgacctgacc ctgcgcgtca acggtggtcg cgttgcgctg     8760

aatttggcgt gtgccattcc gttcagcact cgtgccgcgc agccaagcgc accggaccgc     8820

ctgaccccgg acctgggtgc caaactgccg ctgggcgatc tgctgctgcg tgatgaggac     8880

ggcgcactgt tggcacgtgt gccgcaggct ctgtaccaag actattggac gaatcacggt     8940

attgtggacc tgccgctgct gcgcgaaccg cgtggtagct tgaccctgag cagcgaactg     9000

gcggagtggc gtgagcaaga ctgggtcacc caaagcgacg cgtctaacct gtacctggag     9060

gcaccggatc gccgtcacgg tcgctttttc cctgagagca tcgcgctgcg cagctacttt     9120

cgcggtgaag cgcgtgcgcg tccggatatc ccgcatcgta tcgagggcat gggcctggtc     9180

ggcgtcgaat ctcgtcagga tggcgacgct gcggaatggc gtctgacggg tctgcgtccg     9240

ggtccggcac gcattgttct ggacgatggt gccgaggcga tccctctgcg tgttctgcct     9300

gacgattggg cgctggatga cgcgaccgtc gaagaagtgg attacgcctt tttgtaccgc     9360

cacgttatgg cgtattacga gctggtgtat ccattcatga gcgacaaggt gttttccctg     9420

gctgatcgtt gcaaatgtga aacgtacgca cgtctgatgt ggcagatgtg tgatccgcag     9480

aaccgcaaca agtcctatta catgccgagc acccgcgaac tgtcggcacc gaaagctcgt     9540

ttgttcttga agtatctggc ccacgtggaa ggccaggcac gcctgcaagc acctccgcca     9600

gcgggtccgg cacgcattga atctaaagcc cagttggcgg cagagctgcg taaagccgtc     9660

gacctggagc tgtctgtgat gctgcaatac ctgtacgcgg cgtatagcat tccgaactat     9720

gcacagggcc aacaacgtgt tcgtgacggt gcgtggaccg ccgagcagct gcaactggcg     9780

tgcggtagcg gtgaccgtcg ccgtgatggc ggtattcgtg cagcactgct ggaaattgct     9840

catgaagaaa tgattcatta cctggtcgtt aacaacctgc tgatggccct gggcgagccg     9900

ttctacgcgg gtgtcccgct gatgggcgaa gcggcacgtc aggcgtttgg cctggacacc     9960

gagttcgctc tggaaccgtt tagcgaaagc acgctggcac gttttgttcg tctggaatgg    10020

ccgcacttta tcccagcacc gggcaaatcc atcgcggact gctatgccgc cattcgtcag    10080

gcgtttttgg atctgccgga cttgtttggt ggcgaggcag gtaagcgtgg cggtgaacac    10140

cacctgttcc tgaatgagct gaccaaccgt gcgcatccgg gttatcaact ggaagttttc    10200

gatcgcgact cggcgctgtt tggtattgca tttgtgaccg atcagggcga aggtggcgct    10260

ctggacagcc cgcactacga acatagccat tttcaacgtc tgcgtgaaat gagcgcgcgt    10320

atcatggctc aaagcgcacc gttcgaaccg gcgctgccgg cgttgcgtaa tccggttctg    10380

gatgagagcc cgggttgcca acgtgtcgca gacggtcgtg cgcgtgcgct gatggcattg    10440

taccaaggcg tttatgagct gatgtttgcg atgatggcgc agcacttcgc cgtgaaaccg    10500

ctgggtagct tgcgtcgcag ccgcctgatg aacgcagcaa tcgatctgat gaccggtctg    10560

ttgcgtccgc tgagctgcgc gctgatgaac ctgccaagcg gcatcgccgg tcgcacggcc    10620

ggtccgccgc tgccgggtcc ggttgacacc cgtagctatg acgactacgc gctgggctgt    10680

cgcatgctgg cacgccgttg cgagcgtctg ctggagcagg cgagcatgct ggaaccgggt    10740

tggctgccgg atgcgcagat ggagctgctg gatttctatc gtcgccaaat gctggacttg    10800

gcgtgcggca aactgagccg cgaggcctaa ggatccttaa ggaggtaaaa aaaatgaaac    10860

gtgcgattat cgttggtggc ggcctggcgg gtggcctgac cgcgatctac ctggcgaagc    10920

gtggctacga agtgcacgtc gtggagaagc gtggtgatcc tctgcgcgat ctgagctctt    10980

acgtggacgt tgttagcagc cgtgcgatcg gcgtgagcat gaccgttcgt ggtatcaaga    11040

gcgttttggc tgcgggcatt ccgcgtgcag agctggatgc gtgtggcgaa ccgatcgtgg    11100

caatggcttt ctccgtgggt ggtcagtatc gcatgcgcga actgaagccg ttggaggatt    11160

tccgtccgct gagcttgaac cgtgcggcgt ttcaaaagct gctgaacaaa tacgcgaacc    11220

tggcaggcgt tcgttactac tttgagcata agtgcctgga tgttgacctg gatggtaaga    11280

gcgtgttgat tcagggcaaa gatggtcagc cgcagcgtct gcaaggtgac atgattatcg    11340

gtgcggatgg cgcccacagc gccgtccgtc aggcgatgca gagcggcctg cgtcgtttcg    11400

agttccagca aacgttcttc cgccatggct acaaaaccct ggttttgccg gacgcgcaag    11460

cactgggtta ccgtaaagac acgctgtact ttttcggcat ggattccggt ggcctgttcg    11520

cgggtcgtgc ggctacgatc ccagatggta gcgtcagcat cgccgtttgc ctgccgtact    11580

cgggtagccc ttccctgacg accaccgacg aaccgacgat gcgtgcgttc ttcgatcgtt    11640

acttcggtgg cctgccgcgt gacgcgcgtg acgaaatgct gcgtcagttt ctggcgaagc    11700

cgagcaacga cctgattaac gtgcgctcta gcacctttca ctataagggt aatgtgctgt    11760

tgctgggtga tgctgcgcat gcgactgcgc cgttcctggg tcagggtatg aacatggcgc    11820

tggaggacgc ccgcacgttt gtcgagctgc tggaccgcca ccagggcgac caagacaaag    11880

cctttccgga gttcacggag ctgcgcaaag tccaggcaga cgcaatgcaa gacatggctc    11940

gcgccaacta tgacgttttg agctgctcga acccgatctt tttcatgcgt gcgcgttaca    12000

cgcgttacat gcattccaag tttccgggcc tgtatccgcc ggatatggcc gagaaactgt    12060

actttacgag cgagccgtac gatcgtctgc aacaaatcca gcgtaaacag aatgtttggt    12120

acaagattgg tcgcgtgaat tgaagatctt taaggaggta aaaaaaatga agattctggt    12180

cattggtgct ggtccagctg gtctggtttt cgcatcccaa ctgaagcagg cacgcccttt    12240

gtgggccatt gacatcgtgg agaagaatga cgagcaagaa gtgctgggct ggggtgtcgt    12300

gctgcctggc cgtccgggtc agcacccggc gaacccgctg tcctatctgg atgcaccgga    12360

gcgtctgaat ccgcaatttc tggaggactt caaactggtg catcataatg agccgtcctt    12420

gatgtccacg ggcgttttgt tgtgcggcgt ggagcgtcgc ggtctggttc acgcgctgcg    12480

cgataagtgc cgcagccaag gcattgctat tcgtttcgaa agcccgttgc tggaacacgg    12540

tgagctgccg ctggcggact atgatctggt ggtcctggct aatggtgtta atcacaaaac    12600

cgcgcatttc accgaggctc tggtcccgca ggtggactac ggccgcaata agtacatttg    12660

gtatggcact agccagctgt tcgatcagat gaatctggtt tttcgtaccc atggtaaaga    12720

tatctttatc gcgcatgcct ataagtatag cgataccatg agcacgttca ttgtcgaatg    12780

tagcgaagag acttacgcac gcgcacgcct gggcgaaatg tccgaagagg cgagcgcaga    12840

atacgttgct aaggtgttcc aggccgagct gggtggtcac ggcctggtga gccagccggg    12900

tctgggttgg cgtaacttca tgacgttgtc tcatgaccgt tgtcatgatg gtaagttggt    12960

tctgctgggt gacgcgctgc aaagcggtca ctttagcatc ggccacggca ccacgatggc    13020

cgtggtggtg gcgcagctgc tggttaaagc gctgtgtacc gaagatggtg tgcctgccgc    13080

gctgaaacgt ttcgaagagc gtgccctgcc gctggtgcag ttgttccgtg gccacgcaga    13140

caacagccgc gtttggttcg aaaccgtcga agagcgcatg cacctgtcct cggcggaatt    13200

tgtgcaaagc ttcgacgcac gccgcaaaag cctgccgccg atgccggaag cactggcgca    13260

gaatctgcgt tatgctttgc agcgctgatg atcattaagg aggtaaaaaa aatggagaac    13320

cgtgagccac cactgttgcc agcccgttgg agcagcgcct atgtctctta ttggagcccg    13380

atgctgccgg atgaccagct gaccagcggc tattgctggt tcgactatga acgtgacatc    13440

tgtcgtattg acggcctgtt caatccgtgg agcgagcgtg atactggtta tcgcctgtgg    13500

atgtcggagg ttggtaatgc ggccagcggc cgtacctgga aacaaaaagt cgcctatggt    13560

cgtgagcgta ccgccctggg tgaacagctg tgtgagcgtc cgctggatga tgagactggc    13620

ccttttgccg aattgttcct gccacgcgat gtcctgcgcc gtctgggtgc ccgtcacatt    13680

ggccgtcgcg tggttctggg tcgcgaagcg gacggttggc gttaccagcg cccaggtaaa    13740

ggtccgagca ccctgtacct ggatgcggcg agcggcactc cactgcgcat ggtcaccggc    13800

gatgaagcgt cgcgtgcaag cctgcgtgat tttccgaatg tgagcgaggc ggagatcccg    13860

gacgcggttt tcgcggccaa gcgctaatac tagtagcggc cgctgcagga gtcactaagg    13920

gttagttagt tagattagca gaaagtcaaa agcctccgac cggaggcttt tgactaaaac    13980

ttcccttggg gttatcattg gggctcactc aaaggcggta atcagataaa aaaaatcctt    14040

agctttcgct aaggatgatt tctgctagag atggaataga ctggatggag gcggataaag    14100

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg    14160

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct    14220

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac    14280

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact    14340

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga    14400

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt    14460

cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct    14520

ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact    14580

ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt    14640

agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct    14700

gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag    14760

gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc    14820

tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac    14880

accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag gggaaacgcc    14940

tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga    15000

tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc    15060

tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg    15120

ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc    15180

tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac    15240

ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga    15300

ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc    15360

agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg    15420

attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga    15480

tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc    15540

tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac    15600

ataaacagta atacaagggg tgtttactag aggagattct catgtttgac agcttatcat    15660

cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg    15720

tatgaaatct aacaatgcgc tcatcgtcat tctcggcacc gtcaccctgg acgctgtagg    15780

cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag    15840

tattgccagt cactatggcg tgctgcttgc gctctatgcg ttgatgcaat ttctttgcgc    15900

acccgttctc ggagccctgt ccgaccgctt tggccgccgt ccagtcctgc tcgcttcgct    15960

ccttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggattctcta    16020

cgccggacgc atcgtggcgg gcatcacggg tgccacaggt gcggttgctg gtgcctatat    16080

cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt    16140

cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggtgcca tctccttgca    16200

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctcctcctgg gctgcttcct    16260

tatgcaggaa tcgcataagg gagagcgccg tccgatgccc ttgcgtgcct tcaatccagt    16320

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgttttctt    16380

tatcatgcaa ctcgtaggac aggttccggc agcgctctgg gtcattttcg gcgaggaccg    16440

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc    16500

cctcgctcaa gccttcgtca cgggccccgc caccaaacgt ttcggcgaga agcaggccat    16560

tatcgcgggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgcgg    16620

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggta tgcccgcgtt    16680

gcaggccatg ctgtcccgcc aagtagatga cgaccatcag ggacagcttc aagggtcgct    16740

cgcggctctt accagcctca cttcgatcat tggaccgctg atcgtcacgg cgatttatgc    16800

cgcctcggcg agcacatgga acgggttggc atggattgta ggtgccgccc tttaccttgt    16860

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aataatacta    16920

gctccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa    16980

gattacttcg cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg    17040

ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag    17100

ttagccctta gtgactcgaa ttcgcggccg cttctagag                           17139


