               SEQUENCE LISTING

<110> Kobold, Sebastian
      Endres, Stefan

<120> PD-1-CD28 fusion proteins and their use in medicine

<130> Y1791 PCT S3

<150> EP 15 17 2913.4
<151> 2015-06-19

<160> 65

<170> BiSSAP 1.2

<210> 1
<211> 864
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..864
<223> /mol_type="unassigned DNA"
      /note="murine full-length PD-1"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..864
<223> /transl_table=1

<400> 1
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg gcg gtg    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Ala Val 
            180                 185                 190         

ttt tgc agc acc agc atg agc gaa gcg cgc ggc gcg ggc agc aaa gat    624
Phe Cys Ser Thr Ser Met Ser Glu Ala Arg Gly Ala Gly Ser Lys Asp 
        195                 200                 205             

gat acc ctg aaa gaa gaa ccg agc gcg gcg ccg gtg ccg agc gtg gcg    672
Asp Thr Leu Lys Glu Glu Pro Ser Ala Ala Pro Val Pro Ser Val Ala 
    210                 215                 220                 

tat gaa gaa ctg gat ttt cag ggc cgc gaa aaa acc ccg gaa ctg ccg    720
Tyr Glu Glu Leu Asp Phe Gln Gly Arg Glu Lys Thr Pro Glu Leu Pro 
225                 230                 235                 240 

acc gcg tgc gtg cat acc gaa tat gcg acc att gtg ttt acc gaa ggc    768
Thr Ala Cys Val His Thr Glu Tyr Ala Thr Ile Val Phe Thr Glu Gly 
                245                 250                 255     

ctg ggc gcg agc gcg atg ggc cgc cgc ggc agc gcg gat ggc ctg cag    816
Leu Gly Ala Ser Ala Met Gly Arg Arg Gly Ser Ala Asp Gly Leu Gln 
            260                 265                 270         

ggc ccg cgc ccg ccg cgc cat gaa gat ggc cat tgc agc tgg ccg ctg    864
Gly Pro Arg Pro Pro Arg His Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             

<210> 2
<211> 288
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..864 from SEQ ID NO 1

<400> 2
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Ala Val 
            180                 185                 190         
Phe Cys Ser Thr Ser Met Ser Glu Ala Arg Gly Ala Gly Ser Lys Asp 
        195                 200                 205             
Asp Thr Leu Lys Glu Glu Pro Ser Ala Ala Pro Val Pro Ser Val Ala 
    210                 215                 220                 
Tyr Glu Glu Leu Asp Phe Gln Gly Arg Glu Lys Thr Pro Glu Leu Pro 
225                 230                 235                 240 
Thr Ala Cys Val His Thr Glu Tyr Ala Thr Ile Val Phe Thr Glu Gly 
                245                 250                 255     
Leu Gly Ala Ser Ala Met Gly Arg Arg Gly Ser Ala Asp Gly Leu Gln 
            260                 265                 270         
Gly Pro Arg Pro Pro Arg His Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             



<210> 3
<211> 864
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..864
<223> /mol_type="unassigned DNA"
      /note="human full-length PD-1"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..864
<223> /transl_table=1

<400> 3
atg cag att ccg cag gcg ccg tgg ccg gtg gtg tgg gcg gtg ctg cag     48
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg ggc tgg cgc ccg ggc tgg ttt ctg gat agc ccg gat cgc ccg tgg     96
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          

aac ccg ccg acc ttt agc ccg gcg ctg ctg gtg gtg acc gaa ggc gat    144
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ttt agc aac acc agc gaa agc ttt gtg    192
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  

ctg aac tgg tat cgc atg agc ccg agc aac cag acc gat aaa ctg gcg    240
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  

gcg ttt ccg gaa gat cgc agc cag ccg ggc cag gat tgc cgc ttt cgc    288
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      

gtg acc cag ctg ccg aac ggc cgc gat ttt cat atg agc gtg gtg cgc    336
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         

gcg cgc cgc aac gat agc ggc acc tat ctg tgc ggc gcg att agc ctg    384
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

gcg ccg aaa gcg cag att aaa gaa agc ctg cgc gcg gaa ctg cgc gtg    432
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 

acc gaa cgc cgc gcg gaa gtg ccg acc gcg cat ccg agc ccg agc ccg    480
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 

cgc ccg gcg ggc cag ttt cag acc ctg gtg gtg ggc gtg gtg ggc ggc    528
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     

ctg ctg ggc agc ctg gtg ctg ctg gtg tgg gtg ctg gcg gtg att tgc    576
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         

agc cgc gcg gcg cgc ggc acc att ggc gcg cgc cgc acc ggc cag ccg    624
Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             

ctg aaa gaa gat ccg agc gcg gtg ccg gtg ttt agc gtg gat tat ggc    672
Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 

gaa ctg gat ttt cag tgg cgc gaa aaa acc ccg gaa ccg ccg gtg ccg    720
Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 

tgc gtg ccg gaa cag acc gaa tat gcg acc att gtg ttt ccg agc ggc    768
Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     

atg ggc acc agc agc ccg gcg cgc cgc ggc agc gcg gat ggc ccg cgc    816
Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         

agc gcg cag ccg ctg cgc ccg gaa gat ggc cat tgc agc tgg ccg ctg    864
Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             

<210> 4
<211> 288
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..864 from SEQ ID NO 3

<400> 4
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         
Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             
Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 
Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 
Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     
Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         
Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             



<210> 5
<211> 570
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..570
<223> /mol_type="unassigned DNA"
      /note="murine P1 extracellular and transmembrane domain (AA1-190)
      (PTM construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..570
<223> /transl_table=1

<400> 5
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg            570
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu 
            180                 185                 190 

<210> 6
<211> 190
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..570 from SEQ ID NO 5

<400> 6
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu 
            180                 185                 190 

<210> 7
<211> 507
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..507
<223> /mol_type="unassigned DNA"
      /note="murine PTM PD-1 extracellular domain (without
      transmembrane PD-1 domain)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..507
<223> /transl_table=1

<400> 7
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg                                507
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 8
<211> 169
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..507 from SEQ ID NO 7

<400> 8
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 9
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..63
<223> /mol_type="unassigned DNA"
      /note="murine PTM PD-1 transmembrane domain"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..63
<223> /transl_table=1

<400> 9
gtg att ggc att atg agc gcg ctg gtg ggc att ccg gtg ctg ctg ctg    48
Val Ile Gly Ile Met Ser Ala Leu Val Gly Ile Pro Val Leu Leu Leu 
1               5                   10                  15      

ctg gcg tgg gcg ctg                                                63
Leu Ala Trp Ala Leu 
            20      

<210> 10
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..63 from SEQ ID NO 9

<400> 10
Val Ile Gly Ile Met Ser Ala Leu Val Gly Ile Pro Val Leu Leu Leu 
1               5                   10                  15      
Leu Ala Trp Ala Leu 
            20      

<210> 11
<211> 123
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..123
<223> /mol_type="unassigned DNA"
      /note="murine CD28 intracellular domain (AA178-218) (PTM
      construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..123
<223> /transl_table=1

<400> 11
aac agc cgc cgc aac cgc ctg ctg cag agc gat tat atg aac atg acc     48
Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      

ccg cgc cgc ccg ggc ctg acc cgc aaa ccg tat cag ccg tat gcg ccg     96
Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          

gcg cgc gat ttt gcg gcg tat cgc ccg                                123
Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
        35                  40      

<210> 12
<211> 41
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..123 from SEQ ID NO 11

<400> 12
Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
        35                  40      

<210> 13
<211> 693
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..693
<223> /mol_type="unassigned DNA"
      /note="murine PD1-CD28 (PTM construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..693
<223> /transl_table=1

<400> 13
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg aac agc    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         

cgc cgc aac cgc ctg ctg cag agc gat tat atg aac atg acc ccg cgc    624
Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg 
        195                 200                 205             

cgc ccg ggc ctg acc cgc aaa ccg tat cag ccg tat gcg ccg gcg cgc    672
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg 
    210                 215                 220                 

gat ttt gcg gcg tat cgc ccg                                        693
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 14
<211> 231
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..693 from SEQ ID NO 13

<400> 14
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         
Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg 
        195                 200                 205             
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg 
    210                 215                 220                 
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 15
<211> 573
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..573
<223> /mol_type="unassigned DNA"
      /note="human PTM PD-1 extracellular domain plus transmembrane
      PD-1 domain"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..573
<223> /transl_table=1

<400> 15
atg cag att ccg cag gcg ccg tgg ccg gtg gtg tgg gcg gtg ctg cag     48
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg ggc tgg cgc ccg ggc tgg ttt ctg gat agc ccg gat cgc ccg tgg     96
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          

aac ccg ccg acc ttt agc ccg gcg ctg ctg gtg gtg acc gaa ggc gat    144
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ttt agc aac acc agc gaa agc ttt gtg    192
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  

ctg aac tgg tat cgc atg agc ccg agc aac cag acc gat aaa ctg gcg    240
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  

gcg ttt ccg gaa gat cgc agc cag ccg ggc cag gat tgc cgc ttt cgc    288
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      

gtg acc cag ctg ccg aac ggc cgc gat ttt cat atg agc gtg gtg cgc    336
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         

gcg cgc cgc aac gat agc ggc acc tat ctg tgc ggc gcg att agc ctg    384
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

gcg ccg aaa gcg cag att aaa gaa agc ctg cgc gcg gaa ctg cgc gtg    432
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 

acc gaa cgc cgc gcg gaa gtg ccg acc gcg cat ccg agc ccg agc ccg    480
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 

cgc ccg gcg ggc cag ttt cag acc ctg gtg gtg ggc gtg gtg ggc ggc    528
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     

ctg ctg ggc agc ctg gtg ctg ctg gtg tgg gtg ctg gcg gtg att        573
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile 
            180                 185                 190     

<210> 16
<211> 191
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..573 from SEQ ID NO 15

<400> 16
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile 
            180                 185                 190     

<210> 17
<211> 510
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..510
<223> /mol_type="unassigned DNA"
      /note="human PD1 extracellular domain (without transmembrane
      domain) (PD1-CD28 construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..510
<223> /transl_table=1

<400> 17
atg cag atc cca cag gcg ccc tgg cca gtc gtc tgg gcg gtg cta caa     48
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg ggc tgg cgg cca gga tgg ttc tta gac tcc cca gac agg ccc tgg     96
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          

aac ccc ccc acc ttc tcc cca gcc ctg ctc gtg gtg acc gaa ggg gac    144
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              

aac gcc acc ttc acc tgc agc ttc tcc aac aca tcg gag agc ttc gtg    192
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  

cta aac tgg tac cgc atg agc ccc agc aac cag acg gac aag ctg gcc    240
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  

gcc ttc ccc gag gac cgc agc cag ccc ggc cag gac tgc cgc ttc cgt    288
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      

gtc aca caa ctg ccc aac ggg cgt gac ttc cac atg agc gtg gtc agg    336
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         

gcc cgg cgc aat gac agc ggc acc tac ctc tgt ggg gcc atc tcc ctg    384
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

gcc ccc aag gcg cag atc aaa gag agc ctg cgg gca gag ctc agg gtg    432
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 

aca gag aga agg gca gaa gtg ccc aca gcc cac ccc agc ccc tca ccc    480
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 

agg cca gcc ggc cag ttc caa acc ctg gtg                            510
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val 
                165                 170 

<210> 18
<211> 170
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..510 from SEQ ID NO 17

<400> 18
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val 
                165                 170 

<210> 19
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..63
<223> /mol_type="unassigned DNA"
      /note="human PD1, transmembrane region (PD1-CD28 construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..63
<223> /transl_table=1

<400> 19
gtt ggt gtc gtg ggc ggc ctg ctg ggc agc ctg gtg ctg cta gtc tgg    48
Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp 
1               5                   10                  15      

gtc ctg gcc gtc atc                                                63
Val Leu Ala Val Ile 
            20      

<210> 20
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..63 from SEQ ID NO 19

<400> 20
Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp 
1               5                   10                  15      
Val Leu Ala Val Ile 
            20      

<210> 21
<211> 126
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..126
<223> /mol_type="unassigned DNA"
      /note="human CD28 (PD1-CD28 construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..126
<223> /transl_table=1

<400> 21
agg agt aag agg agc agg ctc ctg cac agt gac tac atg aac atg act     48
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      

ccc cgc cgc ccc ggg ccc acc cgc aag cat tac cag ccc tat gcc cca     96
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          

cca cgc gac ttc gca gcc tat cgc tcc taa                            126
Pro Arg Asp Phe Ala Ala Tyr Arg Ser     
        35                  40          

<210> 22
<211> 41
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..126 from SEQ ID NO 21

<400> 22
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 23
<211> 699
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..699
<223> /mol_type="unassigned DNA"
      /note="human PD1-CD28 construct"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..699
<223> /transl_table=1

<400> 23
atg cag atc cca cag gcg ccc tgg cca gtc gtc tgg gcg gtg cta caa     48
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg ggc tgg cgg cca gga tgg ttc tta gac tcc cca gac agg ccc tgg     96
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          

aac ccc ccc acc ttc tcc cca gcc ctg ctc gtg gtg acc gaa ggg gac    144
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              

aac gcc acc ttc acc tgc agc ttc tcc aac aca tcg gag agc ttc gtg    192
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  

cta aac tgg tac cgc atg agc ccc agc aac cag acg gac aag ctg gcc    240
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  

gcc ttc ccc gag gac cgc agc cag ccc ggc cag gac tgc cgc ttc cgt    288
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      

gtc aca caa ctg ccc aac ggg cgt gac ttc cac atg agc gtg gtc agg    336
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         

gcc cgg cgc aat gac agc ggc acc tac ctc tgt ggg gcc atc tcc ctg    384
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

gcc ccc aag gcg cag atc aaa gag agc ctg cgg gca gag ctc agg gtg    432
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 

aca gag aga agg gca gaa gtg ccc aca gcc cac ccc agc ccc tca ccc    480
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 

agg cca gcc ggc cag ttc caa acc ctg gtg gtt ggt gtc gtg ggc ggc    528
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     

ctg ctg ggc agc ctg gtg ctg cta gtc tgg gtc ctg gcc gtc atc agg    576
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Arg 
            180                 185                 190         

agt aag agg agc agg ctc ctg cac agt gac tac atg aac atg act ccc    624
Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        195                 200                 205             

cgc cgc ccc ggg ccc acc cgc aag cat tac cag ccc tat gcc cca cca    672
Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    210                 215                 220                 

cgc gac ttc gca gcc tat cgc tcc taa                                699
Arg Asp Phe Ala Ala Tyr Arg Ser     
225                 230             

<210> 24
<211> 232
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..699 from SEQ ID NO 23

<400> 24
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     
Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Arg 
            180                 185                 190         
Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        195                 200                 205             
Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    210                 215                 220                 
Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230         

<210> 25
<211> 654
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..654
<223> /mol_type="unassigned DNA"
      /note="murine full-length CD28"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..654
<223> /transl_table=1

<400> 25
atg acc ctg cgc ctg ctg ttt ctg gcg ctg aac ttt ttt agc gtg cag     48
Met Thr Leu Arg Leu Leu Phe Leu Ala Leu Asn Phe Phe Ser Val Gln 
1               5                   10                  15      

gtg acc gaa aac aaa att ctg gtg aaa cag agc ccg ctg ctg gtg gtg     96
Val Thr Glu Asn Lys Ile Leu Val Lys Gln Ser Pro Leu Leu Val Val 
            20                  25                  30          

gat agc aac gaa gtg agc ctg agc tgc cgc tat agc tat aac ctg ctg    144
Asp Ser Asn Glu Val Ser Leu Ser Cys Arg Tyr Ser Tyr Asn Leu Leu 
        35                  40                  45              

gcg aaa gaa ttt cgc gcg agc ctg tat aaa ggc gtg aac agc gat gtg    192
Ala Lys Glu Phe Arg Ala Ser Leu Tyr Lys Gly Val Asn Ser Asp Val 
    50                  55                  60                  

gaa gtg tgc gtg ggc aac ggc aac ttt acc tat cag ccg cag ttt cgc    240
Glu Val Cys Val Gly Asn Gly Asn Phe Thr Tyr Gln Pro Gln Phe Arg 
65                  70                  75                  80  

agc aac gcg gaa ttt aac tgc gat ggc gat ttt gat aac gaa acc gtg    288
Ser Asn Ala Glu Phe Asn Cys Asp Gly Asp Phe Asp Asn Glu Thr Val 
                85                  90                  95      

acc ttt cgc ctg tgg aac ctg cat gtg aac cat acc gat att tat ttt    336
Thr Phe Arg Leu Trp Asn Leu His Val Asn His Thr Asp Ile Tyr Phe 
            100                 105                 110         

tgc aaa att gaa ttt atg tat ccg ccg ccg tat ctg gat aac gaa cgc    384
Cys Lys Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg 
        115                 120                 125             

agc aac ggc acc att att cat att aaa gaa aaa cat ctg tgc cat acc    432
Ser Asn Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr 
    130                 135                 140                 

cag agc agc ccg aaa ctg ttt tgg gcg ctg gtg gtg gtg gcg ggc gtg    480
Gln Ser Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val 
145                 150                 155                 160 

ctg ttt tgc tat ggc ctg ctg gtg acc gtg gcg ctg tgc gtg att tgg    528
Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp 
                165                 170                 175     

acc aac agc cgc cgc aac cgc ctg ctg cag agc gat tat atg aac atg    576
Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met 
            180                 185                 190         

acc ccg cgc cgc ccg ggc ctg acc cgc aaa ccg tat cag ccg tat gcg    624
Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala 
        195                 200                 205             

ccg gcg cgc gat ttt gcg gcg tat cgc ccg                            654
Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
    210                 215             

<210> 26
<211> 218
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..654 from SEQ ID NO 25

<400> 26
Met Thr Leu Arg Leu Leu Phe Leu Ala Leu Asn Phe Phe Ser Val Gln 
1               5                   10                  15      
Val Thr Glu Asn Lys Ile Leu Val Lys Gln Ser Pro Leu Leu Val Val 
            20                  25                  30          
Asp Ser Asn Glu Val Ser Leu Ser Cys Arg Tyr Ser Tyr Asn Leu Leu 
        35                  40                  45              
Ala Lys Glu Phe Arg Ala Ser Leu Tyr Lys Gly Val Asn Ser Asp Val 
    50                  55                  60                  
Glu Val Cys Val Gly Asn Gly Asn Phe Thr Tyr Gln Pro Gln Phe Arg 
65                  70                  75                  80  
Ser Asn Ala Glu Phe Asn Cys Asp Gly Asp Phe Asp Asn Glu Thr Val 
                85                  90                  95      
Thr Phe Arg Leu Trp Asn Leu His Val Asn His Thr Asp Ile Tyr Phe 
            100                 105                 110         
Cys Lys Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg 
        115                 120                 125             
Ser Asn Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr 
    130                 135                 140                 
Gln Ser Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val 
145                 150                 155                 160 
Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp 
                165                 170                 175     
Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met 
            180                 185                 190         
Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala 
        195                 200                 205             
Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
    210                 215             

<210> 27
<211> 660
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..660
<223> /mol_type="unassigned DNA"
      /note="human full-length CD28"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..660
<223> /transl_table=1

<400> 27
atg ctg cgc ctg ctg ctg gcg ctg aac ctg ttt ccg agc att cag gtg     48
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      

acc ggc aac aaa att ctg gtg aaa cag agc ccg atg ctg gtg gcg tat     96
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          

gat aac gcg gtg aac ctg agc tgc aaa tat agc tat aac ctg ttt agc    144
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              

cgc gaa ttt cgc gcg agc ctg cat aaa ggc ctg gat agc gcg gtg gaa    192
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  

gtg tgc gtg gtg tat ggc aac tat agc cag cag ctg cag gtg tat agc    240
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  

aaa acc ggc ttt aac tgc gat ggc aaa ctg ggc aac gaa agc gtg acc    288
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      

ttt tat ctg cag aac ctg tat gtg aac cag acc gat att tat ttt tgc    336
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         

aaa att gaa gtg atg tat ccg ccg ccg tat ctg gat aac gaa aaa agc    384
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             

aac ggc acc att att cat gtg aaa ggc aaa cat ctg tgc ccg agc ccg    432
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 

ctg ttt ccg ggc ccg agc aaa ccg ttt tgg gtg ctg gtg gtg gtg ggc    480
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 

ggc gtg ctg gcg tgc tat agc ctg ctg gtg acc gtg gcg ttt att att    528
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     

ttt tgg gtg cgc agc aaa cgc agc cgc ctg ctg cat agc gat tat atg    576
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         

aac atg acc ccg cgc cgc ccg ggc ccg acc cgc aaa cat tat cag ccg    624
Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             

tat gcg ccg ccg cgc gat ttt gcg gcg tat cgc agc                    660
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 

<210> 28
<211> 220
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..660 from SEQ ID NO 27

<400> 28
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         
Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 

<210> 29
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 domain (not mutated)

<400> 29
Tyr Met Asn Met 
1               

<210> 30
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 domain (not mutated)

<400> 30
Pro Tyr Ala Pro 
1               

<210> 31
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 domain (mutated)

<400> 31
Phe Met Asn Met 
1               

<210> 32
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 domain (mutated)

<400> 32
Ala Tyr Ala Ala 
1               

<210> 33
<211> 870
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..870
<223> /mol_type="unassigned DNA"
      /note="human PDL-1"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..870
<223> /transl_table=1

<400> 33
atg cgc att ttt gcg gtg ttt att ttt atg acc tat tgg cat ctg ctg     48
Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      

aac gcg ttt acc gtg acc gtg ccg aaa gat ctg tat gtg gtg gaa tat     96
Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          

ggc agc aac atg acc att gaa tgc aaa ttt ccg gtg gaa aaa cag ctg    144
Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              

gat ctg gcg gcg ctg att gtg tat tgg gaa atg gaa gat aaa aac att    192
Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  

att cag ttt gtg cat ggc gaa gaa gat ctg aaa gtg cag cat agc agc    240
Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  

tat cgc cag cgc gcg cgc ctg ctg aaa gat cag ctg agc ctg ggc aac    288
Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      

gcg gcg ctg cag att acc gat gtg aaa ctg cag gat gcg ggc gtg tat    336
Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         

cgc tgc atg att agc tat ggc ggc gcg gat tat aaa cgc att acc gtg    384
Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             

aaa gtg aac gcg ccg tat aac aaa att aac cag cgc att ctg gtg gtg    432
Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 

gat ccg gtg acc agc gaa cat gaa ctg acc tgc cag gcg gaa ggc tat    480
Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 

ccg aaa gcg gaa gtg att tgg acc agc agc gat cat cag gtg ctg agc    528
Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     

ggc aaa acc acc acc acc aac agc aaa cgc gaa gaa aaa ctg ttt aac    576
Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         

gtg acc agc acc ctg cgc att aac acc acc acc aac gaa att ttt tat    624
Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             

tgc acc ttt cgc cgc ctg gat ccg gaa gaa aac cat acc gcg gaa ctg    672
Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 

gtg att ccg gaa ctg ccg ctg gcg cat ccg ccg aac gaa cgc acc cat    720
Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 

ctg gtg att ctg ggc gcg att ctg ctg tgc ctg ggc gtg gcg ctg acc    768
Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     

ttt att ttt cgc ctg cgc aaa ggc cgc atg atg gat gtg aaa aaa tgc    816
Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         

ggc att cag gat acc aac agc aaa aaa cag agc gat acc cat ctg gaa    864
Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             

gaa acc                                                            870
Glu Thr 
    290 

<210> 34
<211> 290
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..870 from SEQ ID NO 33

<400> 34
Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      
Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          
Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              
Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  
Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  
Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      
Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         
Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             
Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 
Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 
Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     
Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         
Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             
Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 
Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 
Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     
Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         
Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             
Glu Thr 
    290 

<210> 35
<211> 819
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..819
<223> /mol_type="unassigned DNA"
      /note="human PDL-2"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..819
<223> /transl_table=1

<400> 35
atg att ttt ctg ctg ctg atg ctg agc ctg gaa ctg cag ctg cat cag     48
Met Ile Phe Leu Leu Leu Met Leu Ser Leu Glu Leu Gln Leu His Gln 
1               5                   10                  15      

att gcg gcg ctg ttt acc gtg acc gtg ccg aaa gaa ctg tat att att     96
Ile Ala Ala Leu Phe Thr Val Thr Val Pro Lys Glu Leu Tyr Ile Ile 
            20                  25                  30          

gaa cat ggc agc aac gtg acc ctg gaa tgc aac ttt gat acc ggc agc    144
Glu His Gly Ser Asn Val Thr Leu Glu Cys Asn Phe Asp Thr Gly Ser 
        35                  40                  45              

cat gtg aac ctg ggc gcg att acc gcg agc ctg cag aaa gtg gaa aac    192
His Val Asn Leu Gly Ala Ile Thr Ala Ser Leu Gln Lys Val Glu Asn 
    50                  55                  60                  

gat acc agc ccg cat cgc gaa cgc gcg acc ctg ctg gaa gaa cag ctg    240
Asp Thr Ser Pro His Arg Glu Arg Ala Thr Leu Leu Glu Glu Gln Leu 
65                  70                  75                  80  

ccg ctg ggc aaa gcg agc ttt cat att ccg cag gtg cag gtg cgc gat    288
Pro Leu Gly Lys Ala Ser Phe His Ile Pro Gln Val Gln Val Arg Asp 
                85                  90                  95      

gaa ggc cag tat cag tgc att att att tat ggc gtg gcg tgg gat tat    336
Glu Gly Gln Tyr Gln Cys Ile Ile Ile Tyr Gly Val Ala Trp Asp Tyr 
            100                 105                 110         

aaa tat ctg acc ctg aaa gtg aaa gcg agc tat cgc aaa att aac acc    384
Lys Tyr Leu Thr Leu Lys Val Lys Ala Ser Tyr Arg Lys Ile Asn Thr 
        115                 120                 125             

cat att ctg aaa gtg ccg gaa acc gat gaa gtg gaa ctg acc tgc cag    432
His Ile Leu Lys Val Pro Glu Thr Asp Glu Val Glu Leu Thr Cys Gln 
    130                 135                 140                 

gcg acc ggc tat ccg ctg gcg gaa gtg agc tgg ccg aac gtg agc gtg    480
Ala Thr Gly Tyr Pro Leu Ala Glu Val Ser Trp Pro Asn Val Ser Val 
145                 150                 155                 160 

ccg gcg aac acc agc cat agc cgc acc ccg gaa ggc ctg tat cag gtg    528
Pro Ala Asn Thr Ser His Ser Arg Thr Pro Glu Gly Leu Tyr Gln Val 
                165                 170                 175     

acc agc gtg ctg cgc ctg aaa ccg ccg ccg ggc cgc aac ttt agc tgc    576
Thr Ser Val Leu Arg Leu Lys Pro Pro Pro Gly Arg Asn Phe Ser Cys 
            180                 185                 190         

gtg ttt tgg aac acc cat gtg cgc gaa ctg acc ctg gcg agc att gat    624
Val Phe Trp Asn Thr His Val Arg Glu Leu Thr Leu Ala Ser Ile Asp 
        195                 200                 205             

ctg cag agc cag atg gaa ccg cgc acc cat ccg acc tgg ctg ctg cat    672
Leu Gln Ser Gln Met Glu Pro Arg Thr His Pro Thr Trp Leu Leu His 
    210                 215                 220                 

att ttt att ccg ttt tgc att att gcg ttt att ttt att gcg acc gtg    720
Ile Phe Ile Pro Phe Cys Ile Ile Ala Phe Ile Phe Ile Ala Thr Val 
225                 230                 235                 240 

att gcg ctg cgc aaa cag ctg tgc cag aaa ctg tat agc agc aaa gat    768
Ile Ala Leu Arg Lys Gln Leu Cys Gln Lys Leu Tyr Ser Ser Lys Asp 
                245                 250                 255     

acc acc aaa cgc ccg gtg acc acc acc aaa cgc gaa gtg aac agc gcg    816
Thr Thr Lys Arg Pro Val Thr Thr Thr Lys Arg Glu Val Asn Ser Ala 
            260                 265                 270         

att                                                                819
Ile 
    

<210> 36
<211> 273
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..819 from SEQ ID NO 35

<400> 36
Met Ile Phe Leu Leu Leu Met Leu Ser Leu Glu Leu Gln Leu His Gln 
1               5                   10                  15      
Ile Ala Ala Leu Phe Thr Val Thr Val Pro Lys Glu Leu Tyr Ile Ile 
            20                  25                  30          
Glu His Gly Ser Asn Val Thr Leu Glu Cys Asn Phe Asp Thr Gly Ser 
        35                  40                  45              
His Val Asn Leu Gly Ala Ile Thr Ala Ser Leu Gln Lys Val Glu Asn 
    50                  55                  60                  
Asp Thr Ser Pro His Arg Glu Arg Ala Thr Leu Leu Glu Glu Gln Leu 
65                  70                  75                  80  
Pro Leu Gly Lys Ala Ser Phe His Ile Pro Gln Val Gln Val Arg Asp 
                85                  90                  95      
Glu Gly Gln Tyr Gln Cys Ile Ile Ile Tyr Gly Val Ala Trp Asp Tyr 
            100                 105                 110         
Lys Tyr Leu Thr Leu Lys Val Lys Ala Ser Tyr Arg Lys Ile Asn Thr 
        115                 120                 125             
His Ile Leu Lys Val Pro Glu Thr Asp Glu Val Glu Leu Thr Cys Gln 
    130                 135                 140                 
Ala Thr Gly Tyr Pro Leu Ala Glu Val Ser Trp Pro Asn Val Ser Val 
145                 150                 155                 160 
Pro Ala Asn Thr Ser His Ser Arg Thr Pro Glu Gly Leu Tyr Gln Val 
                165                 170                 175     
Thr Ser Val Leu Arg Leu Lys Pro Pro Pro Gly Arg Asn Phe Ser Cys 
            180                 185                 190         
Val Phe Trp Asn Thr His Val Arg Glu Leu Thr Leu Ala Ser Ile Asp 
        195                 200                 205             
Leu Gln Ser Gln Met Glu Pro Arg Thr His Pro Thr Trp Leu Leu His 
    210                 215                 220                 
Ile Phe Ile Pro Phe Cys Ile Ile Ala Phe Ile Phe Ile Ala Thr Val 
225                 230                 235                 240 
Ile Ala Leu Arg Lys Gln Leu Cys Gln Lys Leu Tyr Ser Ser Lys Asp 
                245                 250                 255     
Thr Thr Lys Arg Pro Val Thr Thr Thr Lys Arg Glu Val Asn Ser Ala 
            260                 265                 270         
Ile 
    

<210> 37
<211> 870
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..870
<223> /mol_type="unassigned DNA"
      /note="full length murine PD-L1 (recombinant murine)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..870
<223> /transl_table=1

<400> 37
atg cgc att ttt gcg ggc att att ttt acc gcg tgc tgc cat ctg ctg     48
Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      

cgc gcg ttt acc att acc gcg ccg aaa gat ctg tat gtg gtg gaa tat     96
Arg Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          

ggc agc aac gtg acc atg gaa tgc cgc ttt ccg gtg gaa cgc gaa ctg    144
Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu 
        35                  40                  45              

gat ctg ctg gcg ctg gtg gtg tat tgg gaa aaa gaa gat gaa cag gtg    192
Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val 
    50                  55                  60                  

att cag ttt gtg gcg ggc gaa gaa gat ctg aaa ccg cag cat agc aac    240
Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn 
65                  70                  75                  80  

ttt cgc ggc cgc gcg agc ctg ccg aaa gat cag ctg ctg aaa ggc aac    288
Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn 
                85                  90                  95      

gcg gcg ctg cag att acc gat gtg aaa ctg cag gat gcg ggc gtg tat    336
Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         

tgc tgc att att agc tat ggc ggc gcg gat tat aaa cgc att acc ctg    384
Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             

aaa gtg aac gcg ccg tat cgc aaa att aac cag cgc att agc gtg gat    432
Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 

ccg gcg acc agc gaa cat gaa ctg att tgc cag gcg gaa ggc tat ccg    480
Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 

gaa gcg gaa gtg att tgg acc aac agc gat cat cag ccg gtg agc ggc    528
Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly 
                165                 170                 175     

aaa cgc agc gtg acc acc agc cgc acc gaa ggc atg ctg ctg aac gtg    576
Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val 
            180                 185                 190         

acc agc agc ctg cgc gtg aac gcg acc gcg aac gat gtg ttt tat tgc    624
Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys 
        195                 200                 205             

acc ttt tgg cgc agc cag ccg ggc cag aac cat acc gcg gaa ctg att    672
Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile 
    210                 215                 220                 

att ccg gaa ctg ccg gcg acc cat ccg ccg cag aac cgc acc cat tgg    720
Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His Trp 
225                 230                 235                 240 

gtg ctg ctg ggc agc att ctg ctg ttt ctg att gtg gtg agc acc gtg    768
Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr Val 
                245                 250                 255     

ctg ctg ttt ctg cgc aaa cag gtg cgc atg ctg gat gtg gaa aaa tgc    816
Leu Leu Phe Leu Arg Lys Gln Val Arg Met Leu Asp Val Glu Lys Cys 
            260                 265                 270         

ggc gtg gaa gat acc agc agc aaa aac cgc aac gat acc cag ttt gaa    864
Gly Val Glu Asp Thr Ser Ser Lys Asn Arg Asn Asp Thr Gln Phe Glu 
        275                 280                 285             

gaa acc                                                            870
Glu Thr 
    290 

<210> 38
<211> 290
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..870 from SEQ ID NO 37

<400> 38
Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      
Arg Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          
Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu 
        35                  40                  45              
Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val 
    50                  55                  60                  
Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn 
65                  70                  75                  80  
Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn 
                85                  90                  95      
Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         
Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             
Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 
Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 
Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly 
                165                 170                 175     
Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val 
            180                 185                 190         
Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys 
        195                 200                 205             
Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile 
    210                 215                 220                 
Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His Trp 
225                 230                 235                 240 
Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr Val 
                245                 250                 255     
Leu Leu Phe Leu Arg Lys Gln Val Arg Met Leu Asp Val Glu Lys Cys 
            260                 265                 270         
Gly Val Glu Asp Thr Ser Ser Lys Asn Arg Asn Asp Thr Gln Phe Glu 
        275                 280                 285             
Glu Thr 
    290 

<210> 39
<211> 507
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..507
<223> /mol_type="unassigned DNA"
      /note="murine PD-1 (AA1-169) (CTM construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..507
<223> /transl_table=1

<400> 39
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg                                507
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 40
<211> 169
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..507 from SEQ ID NO 39

<400> 40
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 41
<211> 204
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..204
<223> /mol_type="unassigned DNA"
      /note="murine CD28 (AA151-218) (CTM construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..204
<223> /transl_table=1

<400> 41
ttt tgg gcg ctg gtg gtg gtg gcg ggc gtg ctg ttt tgc tat ggc ctg     48
Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu 
1               5                   10                  15      

ctg gtg acc gtg gcg ctg tgc gtg att tgg acc aac agc cgc cgc aac     96
Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg Arg Asn 
            20                  25                  30          

cgc ctg ctg cag agc gat tat atg aac atg acc ccg cgc cgc ccg ggc    144
Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              

ctg acc cgc aaa ccg tat cag ccg tat gcg ccg gcg cgc gat ttt gcg    192
Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala 
    50                  55                  60                  

gcg tat cgc ccg                                                    204
Ala Tyr Arg Pro 
65              

<210> 42
<211> 68
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..204 from SEQ ID NO 41

<400> 42
Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu 
1               5                   10                  15      
Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg Arg Asn 
            20                  25                  30          
Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              
Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala 
    50                  55                  60                  
Ala Tyr Arg Pro 
65              

<210> 43
<211> 711
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..711
<223> /mol_type="unassigned DNA"
      /note="murine PD1-CD28 (CTM construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..711
<223> /transl_table=1

<400> 43
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg ttt tgg gcg ctg gtg gtg gtg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Phe Trp Ala Leu Val Val Val 
                165                 170                 175     

gcg ggc gtg ctg ttt tgc tat ggc ctg ctg gtg acc gtg gcg ctg tgc    576
Ala Gly Val Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys 
            180                 185                 190         

gtg att tgg acc aac agc cgc cgc aac cgc ctg ctg cag agc gat tat    624
Val Ile Trp Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr 
        195                 200                 205             

atg aac atg acc ccg cgc cgc ccg ggc ctg acc cgc aaa ccg tat cag    672
Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln 
    210                 215                 220                 

ccg tat gcg ccg gcg cgc gat ttt gcg gcg tat cgc ccg                711
Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
225                 230                 235         

<210> 44
<211> 237
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..711 from SEQ ID NO 43

<400> 44
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Phe Trp Ala Leu Val Val Val 
                165                 170                 175     
Ala Gly Val Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys 
            180                 185                 190         
Val Ile Trp Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr 
        195                 200                 205             
Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln 
    210                 215                 220                 
Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
225                 230                 235         

<210> 45
<211> 507
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..507
<223> /mol_type="unassigned DNA"
      /note="murine PD1 (AA1-169) (CEX construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..507
<223> /transl_table=1

<400> 45
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg                                507
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 46
<211> 169
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..507 from SEQ ID NO 45

<400> 46
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met 
                165                 

<210> 47
<211> 312
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..312
<223> /mol_type="unassigned DNA"
      /note="murine CD28 (AA115-218) (CEX construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..312
<223> /transl_table=1

<400> 47
att gaa ttt atg tat ccg ccg ccg tat ctg gat aac gaa cgc agc aac     48
Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg Ser Asn 
1               5                   10                  15      

ggc acc att att cat att aaa gaa aaa cat ctg tgc cat acc cag agc     96
Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr Gln Ser 
            20                  25                  30          

agc ccg aaa ctg ttt tgg gcg ctg gtg gtg gtg gcg ggc gtg ctg ttt    144
Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe 
        35                  40                  45              

tgc tat ggc ctg ctg gtg acc gtg gcg ctg tgc gtg att tgg acc aac    192
Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn 
    50                  55                  60                  

agc cgc cgc aac cgc ctg ctg cag agc gat tat atg aac atg acc ccg    240
Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro 
65                  70                  75                  80  

cgc cgc ccg ggc ctg acc cgc aaa ccg tat cag ccg tat gcg ccg gcg    288
Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala 
                85                  90                  95      

cgc gat ttt gcg gcg tat cgc ccg                                    312
Arg Asp Phe Ala Ala Tyr Arg Pro 
            100                 

<210> 48
<211> 104
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..312 from SEQ ID NO 47

<400> 48
Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg Ser Asn 
1               5                   10                  15      
Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr Gln Ser 
            20                  25                  30          
Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe 
        35                  40                  45              
Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn 
    50                  55                  60                  
Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro 
65                  70                  75                  80  
Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala 
                85                  90                  95      
Arg Asp Phe Ala Ala Tyr Arg Pro 
            100                 

<210> 49
<211> 819
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..819
<223> /mol_type="unassigned DNA"
      /note="murine PD1-CD28 (CEX construct)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..819
<223> /transl_table=1

<400> 49
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg att gaa ttt atg tat ccg ccg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Ile Glu Phe Met Tyr Pro Pro 
                165                 170                 175     

ccg tat ctg gat aac gaa cgc agc aac ggc acc att att cat att aaa    576
Pro Tyr Leu Asp Asn Glu Arg Ser Asn Gly Thr Ile Ile His Ile Lys 
            180                 185                 190         

gaa aaa cat ctg tgc cat acc cag agc agc ccg aaa ctg ttt tgg gcg    624
Glu Lys His Leu Cys His Thr Gln Ser Ser Pro Lys Leu Phe Trp Ala 
        195                 200                 205             

ctg gtg gtg gtg gcg ggc gtg ctg ttt tgc tat ggc ctg ctg gtg acc    672
Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu Leu Val Thr 
    210                 215                 220                 

gtg gcg ctg tgc gtg att tgg acc aac agc cgc cgc aac cgc ctg ctg    720
Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg Arg Asn Arg Leu Leu 
225                 230                 235                 240 

cag agc gat tat atg aac atg acc ccg cgc cgc ccg ggc ctg acc cgc    768
Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg 
                245                 250                 255     

aaa ccg tat cag ccg tat gcg ccg gcg cgc gat ttt gcg gcg tat cgc    816
Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg 
            260                 265                 270         

ccg                                                                819
Pro 
    

<210> 50
<211> 273
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..819 from SEQ ID NO 49

<400> 50
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Ile Glu Phe Met Tyr Pro Pro 
                165                 170                 175     
Pro Tyr Leu Asp Asn Glu Arg Ser Asn Gly Thr Ile Ile His Ile Lys 
            180                 185                 190         
Glu Lys His Leu Cys His Thr Gln Ser Ser Pro Lys Leu Phe Trp Ala 
        195                 200                 205             
Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu Leu Val Thr 
    210                 215                 220                 
Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg Arg Asn Arg Leu Leu 
225                 230                 235                 240 
Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg 
                245                 250                 255     
Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg 
            260                 265                 270         
Pro 
    

<210> 51
<211> 693
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..693
<223> /mol_type="unassigned DNA"
      /note="PTM-FMNM construct (murine)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..693
<223> /transl_table=1

<400> 51
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg aac agc    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         

cgc cgc aac cgc ctg ctg cag agc gat ttt atg aac atg acc ccg cgc    624
Arg Arg Asn Arg Leu Leu Gln Ser Asp Phe Met Asn Met Thr Pro Arg 
        195                 200                 205             

cgc ccg ggc ctg acc cgc aaa ccg tat cag ccg tat gcg ccg gcg cgc    672
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg 
    210                 215                 220                 

gat ttt gcg gcg tat cgc ccg                                        693
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 52
<211> 231
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..693 from SEQ ID NO 51

<400> 52
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         
Arg Arg Asn Arg Leu Leu Gln Ser Asp Phe Met Asn Met Thr Pro Arg 
        195                 200                 205             
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg 
    210                 215                 220                 
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 53
<211> 693
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..693
<223> /mol_type="unassigned DNA"
      /note="PTM-AYAA construct (murine)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..693
<223> /transl_table=1

<400> 53
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg aac agc    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         

cgc cgc aac cgc ctg ctg cag agc gat tat atg aac atg acc ccg cgc    624
Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg 
        195                 200                 205             

cgc ccg ggc ctg acc cgc aaa ccg tat cag gcg tat gcg gcg gcg cgc    672
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Ala Tyr Ala Ala Ala Arg 
    210                 215                 220                 

gat ttt gcg gcg tat cgc ccg                                        693
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 54
<211> 231
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..693 from SEQ ID NO 53

<400> 54
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         
Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg 
        195                 200                 205             
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Ala Tyr Ala Ala Ala Arg 
    210                 215                 220                 
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 55
<211> 693
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..693
<223> /mol_type="unassigned DNA"
      /note="PTM-FMNM-AYAA construct (murine)"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..693
<223> /transl_table=1

<400> 55
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg aac agc    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         

cgc cgc aac cgc ctg ctg cag agc gat ttt atg aac atg acc ccg cgc    624
Arg Arg Asn Arg Leu Leu Gln Ser Asp Phe Met Asn Met Thr Pro Arg 
        195                 200                 205             

cgc ccg ggc ctg acc cgc aaa ccg tat cag gcg tat gcg gcg gcg cgc    672
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Ala Tyr Ala Ala Ala Arg 
    210                 215                 220                 

gat ttt gcg gcg tat cgc ccg                                        693
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 56
<211> 231
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..693 from SEQ ID NO 55

<400> 56
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Asn Ser 
            180                 185                 190         
Arg Arg Asn Arg Leu Leu Gln Ser Asp Phe Met Asn Met Thr Pro Arg 
        195                 200                 205             
Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Ala Tyr Ala Ala Ala Arg 
    210                 215                 220                 
Asp Phe Ala Ala Tyr Arg Pro 
225                 230     

<210> 57
<211> 741
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..741
<223> /mol_type="unassigned DNA"
      /note="murine PD1 deletion mutant"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..741
<223> /transl_table=1

<400> 57
atg tgg gtg cgc cag gtg ccg tgg agc ttt acc tgg gcg gtg ctg cag     48
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      

ctg agc tgg cag agc ggc tgg ctg ctg gaa gtg ccg aac ggc ccg tgg     96
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          

cgc agc ctg acc ttt tat ccg gcg tgg ctg acc gtg agc gaa ggc gcg    144
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              

aac gcg acc ttt acc tgc agc ctg agc aac tgg agc gaa gat ctg atg    192
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  

ctg aac tgg aac cgc ctg agc ccg agc aac cag acc gaa aaa cag gcg    240
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  

gcg ttt tgc aac ggc ctg agc cag ccg gtg cag gat gcg cgc ttt cag    288
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      

att att cag ctg ccg aac cgc cat gat ttt cat atg aac att ctg gat    336
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         

acc cgc cgc aac gat agc ggc att tat ctg tgc ggc gcg att agc ctg    384
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             

cat ccg aaa gcg aaa att gaa gaa agc ccg ggc gcg gaa ctg gtg gtg    432
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 

acc gaa cgc att ctg gaa acc agc acc cgc tat ccg agc ccg agc ccg    480
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 

aaa ccg gaa ggc cgc ttt cag ggc atg gtg att ggc att atg agc gcg    528
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     

ctg gtg ggc att ccg gtg ctg ctg ctg ctg gcg tgg gcg ctg gcg gtg    576
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Ala Val 
            180                 185                 190         

ttt tgc agc acc agc atg agc gaa gcg cgc ggc gcg ggc agc aaa gat    624
Phe Cys Ser Thr Ser Met Ser Glu Ala Arg Gly Ala Gly Ser Lys Asp 
        195                 200                 205             

gat acc ctg aaa gaa gaa ccg agc gcg gcg ccg gtg ccg agc gtg gcg    672
Asp Thr Leu Lys Glu Glu Pro Ser Ala Ala Pro Val Pro Ser Val Ala 
    210                 215                 220                 

tat gaa gaa ctg gat ttt cag ggc cgc gaa aaa acc ccg gaa ctg ccg    720
Tyr Glu Glu Leu Asp Phe Gln Gly Arg Glu Lys Thr Pro Glu Leu Pro 
225                 230                 235                 240 

acc gcg tgc gtg cat acc gaa                                        741
Thr Ala Cys Val His Thr Glu 
                245         

<210> 58
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..741 from SEQ ID NO 57

<400> 58
Met Trp Val Arg Gln Val Pro Trp Ser Phe Thr Trp Ala Val Leu Gln 
1               5                   10                  15      
Leu Ser Trp Gln Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp 
            20                  25                  30          
Arg Ser Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala 
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
    50                  55                  60                  
Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
65                  70                  75                  80  
Ala Phe Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
                85                  90                  95      
Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
            100                 105                 110         
Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             
His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
    130                 135                 140                 
Thr Glu Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro 
145                 150                 155                 160 
Lys Pro Glu Gly Arg Phe Gln Gly Met Val Ile Gly Ile Met Ser Ala 
                165                 170                 175     
Leu Val Gly Ile Pro Val Leu Leu Leu Leu Ala Trp Ala Leu Ala Val 
            180                 185                 190         
Phe Cys Ser Thr Ser Met Ser Glu Ala Arg Gly Ala Gly Ser Lys Asp 
        195                 200                 205             
Asp Thr Leu Lys Glu Glu Pro Ser Ala Ala Pro Val Pro Ser Val Ala 
    210                 215                 220                 
Tyr Glu Glu Leu Asp Phe Gln Gly Arg Glu Lys Thr Pro Glu Leu Pro 
225                 230                 235                 240 
Thr Ala Cys Val His Thr Glu 
                245         

<210> 59
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..29
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 59
atagcggccg cgccaccatg tgggtccgg                                      29


<210> 60
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 60
ccttctacta ttgcagaaga cag                                            23


<210> 61
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 61
ctgtcttctg caatagtaga agg                                            23


<210> 62
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..28
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 62
tatgaattct caggggcggt acgctgca                                       28


<210> 63
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..29
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 63
atagcggccg cgccaccatg tgggtccgg                                      29


<210> 64
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..28
<223> /mol_type="unassigned DNA"
      /note="Primer"
      /organism="Artificial Sequence"

<400> 64
tatgaattct caggggcggt acgctgca                                       28


<210> 65
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Control peptide

<400> 65
Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               

