                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
       Jean-Marc, NICAUD
       Zbigniew, LAZAR
       Anne-Marie, CRUTZ-LE COQ
       Heber, GAMBOA-MELENDEZ
       Ccile, NEUVEGLISE
 
<120>  MUTANT YARROWIA STRAIN CAPABLE OF DEGRADING GALACTOSE

<130>  XARA/brvi-F539_179/PCT

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  1431
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1431)

<400>  1
atg tct atc agc acc ctg ccg ctg gcg aac atc tac gcg gag ccg acc         48
Met Ser Ile Ser Thr Leu Pro Leu Ala Asn Ile Tyr Ala Glu Pro Thr           
1               5                   10                  15                

aag cag cag ccg cgc tat gat cag ctg act aag cgg ttt tcg gag ctg         96
Lys Gln Gln Pro Arg Tyr Asp Gln Leu Thr Lys Arg Phe Ser Glu Leu           
            20                  25                  30                    

tac gca gtc tcc agc ccg act ttg gtg aga tcg ccg ggc cga gtc aac        144
Tyr Ala Val Ser Ser Pro Thr Leu Val Arg Ser Pro Gly Arg Val Asn           
        35                  40                  45                        

ctc att ggc gag cac att gac tac tcc cac ttc agt gtc atg ccc atg        192
Leu Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met           
    50                  55                  60                            

gcg att gag gat gac gtc atc atg gcc ttc agc gtt ggc ggc gac tcc        240
Ala Ile Glu Asp Asp Val Ile Met Ala Phe Ser Val Gly Gly Asp Ser           
65                  70                  75                  80            

att gag att gcc aac atg gaa gac gac aag ttc ccg gca gaa aag tac        288
Ile Glu Ile Ala Asn Met Glu Asp Asp Lys Phe Pro Ala Glu Lys Tyr           
                85                  90                  95                

aac gtt ccg acc gcc gac cag gtg ctc act atc gac cag aca aaa aca        336
Asn Val Pro Thr Ala Asp Gln Val Leu Thr Ile Asp Gln Thr Lys Thr           
            100                 105                 110                   

gtg tgg acc aac tac ttc aaa tgt gga cac aca gtg ggc cga aag cat        384
Val Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Gly Arg Lys His           
        115                 120                 125                       

ctg aga gac tct gga gag gag gta aaa ccc gtg gga atc aag gtg ttg        432
Leu Arg Asp Ser Gly Glu Glu Val Lys Pro Val Gly Ile Lys Val Leu           
    130                 135                 140                           

gtc cat gga act gtc ccc gac gcc tgt gga ctg tcc tct tcc gct gcc        480
Val His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala           
145                 150                 155                 160           

ttt gtg gtg tgc tcc act ctc gtc act ctt ctg tcc cag ggc atg cag        528
Phe Val Val Cys Ser Thr Leu Val Thr Leu Leu Ser Gln Gly Met Gln           
                165                 170                 175               

gag gtg tca cgt gag ctg ctc acc agg ctc tcc atc acg tgc gag cag        576
Glu Val Ser Arg Glu Leu Leu Thr Arg Leu Ser Ile Thr Cys Glu Gln           
            180                 185                 190                   

ctt att ggt gtc aac acc ggc ggt atg gac cag gct gcc tcc atc tac        624
Leu Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile Tyr           
        195                 200                 205                       

ggc caa ctc aat cat act ctc ctg gtg gat ttt gct cca acc ctc aag        672
Gly Gln Leu Asn His Thr Leu Leu Val Asp Phe Ala Pro Thr Leu Lys           
    210                 215                 220                           

gcc act cca ttc cag ttc ccc tca acg acc ccc aag acc gtt ttt ctg        720
Ala Thr Pro Phe Gln Phe Pro Ser Thr Thr Pro Lys Thr Val Phe Leu           
225                 230                 235                 240           

gtt gcc aac acg ctg gtc aag tcc aac aaa cac gaa acc gcg cct cga        768
Val Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr Ala Pro Arg           
                245                 250                 255               

aac tac aat ctg cgg gtt gtg gag atc act ctg gcc gtc aac gtg ctt        816
Asn Tyr Asn Leu Arg Val Val Glu Ile Thr Leu Ala Val Asn Val Leu           
            260                 265                 270                   

ctg aag agt ctc aag gga gtc acc att cct cag aac gga aac ctc aac        864
Leu Lys Ser Leu Lys Gly Val Thr Ile Pro Gln Asn Gly Asn Leu Asn           
        275                 280                 285                       

aag gga act ctt cac gga ctt atg aag gct gtt gat act gac ctg cac        912
Lys Gly Thr Leu His Gly Leu Met Lys Ala Val Asp Thr Asp Leu His           
    290                 295                 300                           

gga ctt ttg gac ctg gtg gaa act ctg ccc aag gag cct gtg act ctg        960
Gly Leu Leu Asp Leu Val Glu Thr Leu Pro Lys Glu Pro Val Thr Leu           
305                 310                 315                 320           

caa gag gtg gcc ggc ctg ctg aac gtc tct gag gac gac gtc aag aag       1008
Gln Glu Val Ala Gly Leu Leu Asn Val Ser Glu Asp Asp Val Lys Lys           
                325                 330                 335               

cag tac ttc att ttc ccc gtc gaa ttc gac act ctg gct ctc tac tcg       1056
Gln Tyr Phe Ile Phe Pro Val Glu Phe Asp Thr Leu Ala Leu Tyr Ser           
            340                 345                 350                   

cgg gcc aag cat gtt gtc aca gag gcc att cga gtc aag gag ttt caa       1104
Arg Ala Lys His Val Val Thr Glu Ala Ile Arg Val Lys Glu Phe Gln           
        355                 360                 365                       

aac ctg ctg tcc aag ggc gag tct gac ccc agg cag ctc ggc aag ctc       1152
Asn Leu Leu Ser Lys Gly Glu Ser Asp Pro Arg Gln Leu Gly Lys Leu           
    370                 375                 380                           

atg aac gag agt caa aag tcg tgc gac cag ctc ttc aac tgc tcc tgt       1200
Met Asn Glu Ser Gln Lys Ser Cys Asp Gln Leu Phe Asn Cys Ser Cys           
385                 390                 395                 400           

cct gcc atc gac aag gtg tgc gac att gtg ctc aat tct gga ggg ttt       1248
Pro Ala Ile Asp Lys Val Cys Asp Ile Val Leu Asn Ser Gly Gly Phe           
                405                 410                 415               

gga tcg cgt ctg act ggt gcg ggg tgg gga gga tct act gtc cat ctg       1296
Gly Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His Leu           
            420                 425                 430                   

gtt gag gaa gcc aag ctg aac aac gtg att gac gct ctg aag aag gaa       1344
Val Glu Glu Ala Lys Leu Asn Asn Val Ile Asp Ala Leu Lys Lys Glu           
        435                 440                 445                       

tac tac agc gac atg gat ggt gtc gat tac gat agc gcc atg gtc gtc       1392
Tyr Tyr Ser Asp Met Asp Gly Val Asp Tyr Asp Ser Ala Met Val Val           
    450                 455                 460                           

act gtg cct ggc gag gga tgt gct att gtt gat ttg taa                   1431
Thr Val Pro Gly Glu Gly Cys Ala Ile Val Asp Leu                           
465                 470                 475                               


<210>  2
<211>  476
<212>  PRT
<213>  Yarrowia lipolytica

<400>  2

Met Ser Ile Ser Thr Leu Pro Leu Ala Asn Ile Tyr Ala Glu Pro Thr 
1               5                   10                  15      


Lys Gln Gln Pro Arg Tyr Asp Gln Leu Thr Lys Arg Phe Ser Glu Leu 
            20                  25                  30          


Tyr Ala Val Ser Ser Pro Thr Leu Val Arg Ser Pro Gly Arg Val Asn 
        35                  40                  45              


Leu Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met 
    50                  55                  60                  


Ala Ile Glu Asp Asp Val Ile Met Ala Phe Ser Val Gly Gly Asp Ser 
65                  70                  75                  80  


Ile Glu Ile Ala Asn Met Glu Asp Asp Lys Phe Pro Ala Glu Lys Tyr 
                85                  90                  95      


Asn Val Pro Thr Ala Asp Gln Val Leu Thr Ile Asp Gln Thr Lys Thr 
            100                 105                 110         


Val Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Gly Arg Lys His 
        115                 120                 125             


Leu Arg Asp Ser Gly Glu Glu Val Lys Pro Val Gly Ile Lys Val Leu 
    130                 135                 140                 


Val His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala 
145                 150                 155                 160 


Phe Val Val Cys Ser Thr Leu Val Thr Leu Leu Ser Gln Gly Met Gln 
                165                 170                 175     


Glu Val Ser Arg Glu Leu Leu Thr Arg Leu Ser Ile Thr Cys Glu Gln 
            180                 185                 190         


Leu Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile Tyr 
        195                 200                 205             


Gly Gln Leu Asn His Thr Leu Leu Val Asp Phe Ala Pro Thr Leu Lys 
    210                 215                 220                 


Ala Thr Pro Phe Gln Phe Pro Ser Thr Thr Pro Lys Thr Val Phe Leu 
225                 230                 235                 240 


Val Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr Ala Pro Arg 
                245                 250                 255     


Asn Tyr Asn Leu Arg Val Val Glu Ile Thr Leu Ala Val Asn Val Leu 
            260                 265                 270         


Leu Lys Ser Leu Lys Gly Val Thr Ile Pro Gln Asn Gly Asn Leu Asn 
        275                 280                 285             


Lys Gly Thr Leu His Gly Leu Met Lys Ala Val Asp Thr Asp Leu His 
    290                 295                 300                 


Gly Leu Leu Asp Leu Val Glu Thr Leu Pro Lys Glu Pro Val Thr Leu 
305                 310                 315                 320 


Gln Glu Val Ala Gly Leu Leu Asn Val Ser Glu Asp Asp Val Lys Lys 
                325                 330                 335     


Gln Tyr Phe Ile Phe Pro Val Glu Phe Asp Thr Leu Ala Leu Tyr Ser 
            340                 345                 350         


Arg Ala Lys His Val Val Thr Glu Ala Ile Arg Val Lys Glu Phe Gln 
        355                 360                 365             


Asn Leu Leu Ser Lys Gly Glu Ser Asp Pro Arg Gln Leu Gly Lys Leu 
    370                 375                 380                 


Met Asn Glu Ser Gln Lys Ser Cys Asp Gln Leu Phe Asn Cys Ser Cys 
385                 390                 395                 400 


Pro Ala Ile Asp Lys Val Cys Asp Ile Val Leu Asn Ser Gly Gly Phe 
                405                 410                 415     


Gly Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His Leu 
            420                 425                 430         


Val Glu Glu Ala Lys Leu Asn Asn Val Ile Asp Ala Leu Lys Lys Glu 
        435                 440                 445             


Tyr Tyr Ser Asp Met Asp Gly Val Asp Tyr Asp Ser Ala Met Val Val 
    450                 455                 460                 


Thr Val Pro Gly Glu Gly Cys Ala Ile Val Asp Leu 
465                 470                 475     


<210>  3
<211>  1059
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1059)

<400>  3
atg act ctt gtg gca tcc gac att cct cac aga cga tac aac ccg ctg         48
Met Thr Leu Val Ala Ser Asp Ile Pro His Arg Arg Tyr Asn Pro Leu           
1               5                   10                  15                

cga caa tca tgg gtg ctg gtg tca cct cat cga gct aag cga cct tgg         96
Arg Gln Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp           
            20                  25                  30                    

cag gga cag caa gaa gag ccc acc aag gac acg aag ccc gag tac gac        144
Gln Gly Gln Gln Glu Glu Pro Thr Lys Asp Thr Lys Pro Glu Tyr Asp           
        35                  40                  45                        

gac aag tgc tat ctg tgt cct cga aat gct cga atc aac ggg gag atg        192
Asp Lys Cys Tyr Leu Cys Pro Arg Asn Ala Arg Ile Asn Gly Glu Met           
    50                  55                  60                            

aac ccg gac tac aag gac act ttc gtg ttt gag aac gac ttc agt gct        240
Asn Pro Asp Tyr Lys Asp Thr Phe Val Phe Glu Asn Asp Phe Ser Ala           
65                  70                  75                  80            

gtt ttt gag aaa act gcg gaa gtg gag aag gtc gtt gag gag gcc caa        288
Val Phe Glu Lys Thr Ala Glu Val Glu Lys Val Val Glu Glu Ala Gln           
                85                  90                  95                

aag gag cac aca gag tct act gct ctc gac ctg gac ctt ctg cga aaa        336
Lys Glu His Thr Glu Ser Thr Ala Leu Asp Leu Asp Leu Leu Arg Lys           
            100                 105                 110                   

gaa gat gtg ttc gga tac tgt cag gtt att tgc ttt tcg ccc aaa cac        384
Glu Asp Val Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser Pro Lys His           
        115                 120                 125                       

aat gtg act ctt gct aca atg act gtt gag cag atc gaa aag gtg att        432
Asn Val Thr Leu Ala Thr Met Thr Val Glu Gln Ile Glu Lys Val Ile           
    130                 135                 140                           

gag acc tgg cag aaa atg tac aca gag gct cag agt cga gga atc aag        480
Glu Thr Trp Gln Lys Met Tyr Thr Glu Ala Gln Ser Arg Gly Ile Lys           
145                 150                 155                 160           

tac tcg ctg att ttt gaa aac aag gga gct acc atg ggc tgc tcc aac        528
Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly Cys Ser Asn           
                165                 170                 175               

cct cat gcc cat ggc cag gtc tgg aac acc agt att gtt cct gaa gag        576
Pro His Ala His Gly Gln Val Trp Asn Thr Ser Ile Val Pro Glu Glu           
            180                 185                 190                   

ccc cag atg gag ttt gat tca ttg aag aaa tac aag gac gaa aag aac        624
Pro Gln Met Glu Phe Asp Ser Leu Lys Lys Tyr Lys Asp Glu Lys Asn           
        195                 200                 205                       

cgt gat ctt ctg gtc gat tac gtc aac ttg gaa atg tcc aaa aac gaa        672
Arg Asp Leu Leu Val Asp Tyr Val Asn Leu Glu Met Ser Lys Asn Glu           
    210                 215                 220                           

cga atc gtc tac cag aat gcc tcg ttt ctc gtg gtg gtt cct tac tgg        720
Arg Ile Val Tyr Gln Asn Ala Ser Phe Leu Val Val Val Pro Tyr Trp           
225                 230                 235                 240           

gcc gtc tgg cct ttt gag acc atg att gtg tcc aag gaa cgg gtt tct        768
Ala Val Trp Pro Phe Glu Thr Met Ile Val Ser Lys Glu Arg Val Ser           
                245                 250                 255               

tcc atc aaa aag ctg acc aag gaa caa gtc cag cat ctt gcc gac gcc        816
Ser Ile Lys Lys Leu Thr Lys Glu Gln Val Gln His Leu Ala Asp Ala           
            260                 265                 270                   

atc tcg gtg atc aca aaa aag tac gac aag ctc ttc aac acc tct ttc        864
Ile Ser Val Ile Thr Lys Lys Tyr Asp Lys Leu Phe Asn Thr Ser Phe           
        275                 280                 285                       

ccc tac tca atg ggt att cat caa tca ttt gtg gac gtt gag aac tgt        912
Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Val Glu Asn Cys           
    290                 295                 300                           

ccc tac gag cat ctt cgg ctc ctg ttc tac cct cct ctt ctg cga tct        960
Pro Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu Leu Arg Ser           
305                 310                 315                 320           

gcc tcc gtc aag aag ttc cag gtc gga ttc gag atg ctg gga atg ccg       1008
Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu Gly Met Pro           
                325                 330                 335               

cag cga gac att act gcc gaa ggg gct gcc aag cgg ttg tat gat ttg       1056
Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Lys Arg Leu Tyr Asp Leu           
            340                 345                 350                   

taa                                                                   1059


<210>  4
<211>  352
<212>  PRT
<213>  Yarrowia lipolytica

<400>  4

Met Thr Leu Val Ala Ser Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Gln Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Thr Lys Asp Thr Lys Pro Glu Tyr Asp 
        35                  40                  45              


Asp Lys Cys Tyr Leu Cys Pro Arg Asn Ala Arg Ile Asn Gly Glu Met 
    50                  55                  60                  


Asn Pro Asp Tyr Lys Asp Thr Phe Val Phe Glu Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Glu Lys Thr Ala Glu Val Glu Lys Val Val Glu Glu Ala Gln 
                85                  90                  95      


Lys Glu His Thr Glu Ser Thr Ala Leu Asp Leu Asp Leu Leu Arg Lys 
            100                 105                 110         


Glu Asp Val Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser Pro Lys His 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Met Thr Val Glu Gln Ile Glu Lys Val Ile 
    130                 135                 140                 


Glu Thr Trp Gln Lys Met Tyr Thr Glu Ala Gln Ser Arg Gly Ile Lys 
145                 150                 155                 160 


Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly Cys Ser Asn 
                165                 170                 175     


Pro His Ala His Gly Gln Val Trp Asn Thr Ser Ile Val Pro Glu Glu 
            180                 185                 190         


Pro Gln Met Glu Phe Asp Ser Leu Lys Lys Tyr Lys Asp Glu Lys Asn 
        195                 200                 205             


Arg Asp Leu Leu Val Asp Tyr Val Asn Leu Glu Met Ser Lys Asn Glu 
    210                 215                 220                 


Arg Ile Val Tyr Gln Asn Ala Ser Phe Leu Val Val Val Pro Tyr Trp 
225                 230                 235                 240 


Ala Val Trp Pro Phe Glu Thr Met Ile Val Ser Lys Glu Arg Val Ser 
                245                 250                 255     


Ser Ile Lys Lys Leu Thr Lys Glu Gln Val Gln His Leu Ala Asp Ala 
            260                 265                 270         


Ile Ser Val Ile Thr Lys Lys Tyr Asp Lys Leu Phe Asn Thr Ser Phe 
        275                 280                 285             


Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Val Glu Asn Cys 
    290                 295                 300                 


Pro Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu Leu Arg Ser 
305                 310                 315                 320 


Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu Gly Met Pro 
                325                 330                 335     


Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Lys Arg Leu Tyr Asp Leu 
            340                 345                 350         


<210>  5
<211>  1110
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1110)

<400>  5
atg acc cag gct gct gct gaa cac gtg ctc gtc acc gga gga gcc gga         48
Met Thr Gln Ala Ala Ala Glu His Val Leu Val Thr Gly Gly Ala Gly           
1               5                   10                  15                

tac atc ggc tct cac act gtt att gct ctg gtt gag gct gga cga aag         96
Tyr Ile Gly Ser His Thr Val Ile Ala Leu Val Glu Ala Gly Arg Lys           
            20                  25                  30                    

cca gtc att gtc gac aac ctg gac aac tcg tgt gag gag gct gtg cgc        144
Pro Val Ile Val Asp Asn Leu Asp Asn Ser Cys Glu Glu Ala Val Arg           
        35                  40                  45                        

cga atc gaa cag att gtg ggc gag aaa atc ccc ttc ttt gag gtt gat        192
Arg Ile Glu Gln Ile Val Gly Glu Lys Ile Pro Phe Phe Glu Val Asp           
    50                  55                  60                            

atc aga gac act aac ggt ctg gat gaa gtg tac aag cag tac cca acc        240
Ile Arg Asp Thr Asn Gly Leu Asp Glu Val Tyr Lys Gln Tyr Pro Thr           
65                  70                  75                  80            

atc acc tct gtg ctg cat ttc gct ggt ctc aag gcc gtg gga gaa tct        288
Ile Thr Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser           
                85                  90                  95                

ggc cag att cct ctg gag tat tac aac tgc aac gtg gcg ggt tcc att        336
Gly Gln Ile Pro Leu Glu Tyr Tyr Asn Cys Asn Val Ala Gly Ser Ile           
            100                 105                 110                   

aac ctc ctg cag tgg tgc cag aag cac aac atc cac aac ttt gtc ttc        384
Asn Leu Leu Gln Trp Cys Gln Lys His Asn Ile His Asn Phe Val Phe           
        115                 120                 125                       

tcg tcg tcc gcc acc gtc tac gga gac gtc acc cgg ttt gag ggc atg        432
Ser Ser Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met           
    130                 135                 140                           

att ccc att cct gag gag tgt ccc acc gga ccc act aac cct tac gga        480
Ile Pro Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly           
145                 150                 155                 160           

cga acc aag atg atg atc gag gac atc att cga gat cac gtg cga gcc        528
Arg Thr Lys Met Met Ile Glu Asp Ile Ile Arg Asp His Val Arg Ala           
                165                 170                 175               

aat gct ccc tgg aac gcc gct att ctg cga tac ttc aat ccg atg ggt        576
Asn Ala Pro Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly           
            180                 185                 190                   

gcc cat ccc tct ggt ctg gtt gga gag gat cct ctt ggt atc ccc aac        624
Ala His Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn           
        195                 200                 205                       

aac ctg ctt cct tac ctt gcc cag gtt gcc gtg ggc cga cga gag aag        672
Asn Leu Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys           
    210                 215                 220                           

ctc ttt gtg ttt ggt gac gac tac ccc tct cga gat gga act ccc atc        720
Leu Phe Val Phe Gly Asp Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile           
225                 230                 235                 240           

cga gac tac atc cac gtg tgc gat ctt gct gct ggc cac gtg gct gcc        768
Arg Asp Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala           
                245                 250                 255               

ctt gag aag ctc gag gct gtc gat aaa gag cga aag cag ctg ccc cag        816
Leu Glu Lys Leu Glu Ala Val Asp Lys Glu Arg Lys Gln Leu Pro Gln           
            260                 265                 270                   

ggg tcc ccc gag ctc aag cac ggt ttc tct cga gaa tgg aac cta gga        864
Gly Ser Pro Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly           
        275                 280                 285                       

acc ggc cac gga tct act gtg ctt gag gtc gtc aag gcc ttt tcc aac        912
Thr Gly His Gly Ser Thr Val Leu Glu Val Val Lys Ala Phe Ser Asn           
    290                 295                 300                           

gcc gtg ggt aag gac cta ccc tac gaa atc acc ggt cga cga gac gga        960
Ala Val Gly Lys Asp Leu Pro Tyr Glu Ile Thr Gly Arg Arg Asp Gly           
305                 310                 315                 320           

gac gtt ctg gat ctc acc gcg aaa ccc ggc cga gca aac tcc gag ctc       1008
Asp Val Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Ser Glu Leu           
                325                 330                 335               

aac tgg aag gca gag aag act ctc gac gaa agc tgc tcc gat ctg tgg       1056
Asn Trp Lys Ala Glu Lys Thr Leu Asp Glu Ser Cys Ser Asp Leu Trp           
            340                 345                 350                   

aga tgg acc agc gag aac ccc tat ggt tac aaa aag gac cgt cat gac       1104
Arg Trp Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Lys Asp Arg His Asp           
        355                 360                 365                       

aag taa                                                               1110
Lys                                                                       
                                                                          


<210>  6
<211>  369
<212>  PRT
<213>  Yarrowia lipolytica

<400>  6

Met Thr Gln Ala Ala Ala Glu His Val Leu Val Thr Gly Gly Ala Gly 
1               5                   10                  15      


Tyr Ile Gly Ser His Thr Val Ile Ala Leu Val Glu Ala Gly Arg Lys 
            20                  25                  30          


Pro Val Ile Val Asp Asn Leu Asp Asn Ser Cys Glu Glu Ala Val Arg 
        35                  40                  45              


Arg Ile Glu Gln Ile Val Gly Glu Lys Ile Pro Phe Phe Glu Val Asp 
    50                  55                  60                  


Ile Arg Asp Thr Asn Gly Leu Asp Glu Val Tyr Lys Gln Tyr Pro Thr 
65                  70                  75                  80  


Ile Thr Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser 
                85                  90                  95      


Gly Gln Ile Pro Leu Glu Tyr Tyr Asn Cys Asn Val Ala Gly Ser Ile 
            100                 105                 110         


Asn Leu Leu Gln Trp Cys Gln Lys His Asn Ile His Asn Phe Val Phe 
        115                 120                 125             


Ser Ser Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met 
    130                 135                 140                 


Ile Pro Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly 
145                 150                 155                 160 


Arg Thr Lys Met Met Ile Glu Asp Ile Ile Arg Asp His Val Arg Ala 
                165                 170                 175     


Asn Ala Pro Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly 
            180                 185                 190         


Ala His Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn 
        195                 200                 205             


Asn Leu Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys 
    210                 215                 220                 


Leu Phe Val Phe Gly Asp Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile 
225                 230                 235                 240 


Arg Asp Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala 
                245                 250                 255     


Leu Glu Lys Leu Glu Ala Val Asp Lys Glu Arg Lys Gln Leu Pro Gln 
            260                 265                 270         


Gly Ser Pro Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly 
        275                 280                 285             


Thr Gly His Gly Ser Thr Val Leu Glu Val Val Lys Ala Phe Ser Asn 
    290                 295                 300                 


Ala Val Gly Lys Asp Leu Pro Tyr Glu Ile Thr Gly Arg Arg Asp Gly 
305                 310                 315                 320 


Asp Val Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Ser Glu Leu 
                325                 330                 335     


Asn Trp Lys Ala Glu Lys Thr Leu Asp Glu Ser Cys Ser Asp Leu Trp 
            340                 345                 350         


Arg Trp Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Lys Asp Arg His Asp 
        355                 360                 365             


Lys 
    


<210>  7
<211>  984
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(984)

<400>  7
atg acc ttt tgc aac acg gga gca acg ctc cag agc tgg gaa aat agc         48
Met Thr Phe Cys Asn Thr Gly Ala Thr Leu Gln Ser Trp Glu Asn Ser           
1               5                   10                  15                

atc ctc ggg ttt gaa aac cac aca aat tac ctg aac cga caa aac ccc         96
Ile Leu Gly Phe Glu Asn His Thr Asn Tyr Leu Asn Arg Gln Asn Pro           
            20                  25                  30                    

tac ttt gga gct aca att ggc ccg gta gct aac cgg cta tac caa cca        144
Tyr Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr Gln Pro           
        35                  40                  45                        

aac ggt atc ctg ttg cac ggc gga aaa gaa ggc tgg tcc tgg aag aaa        192
Asn Gly Ile Leu Leu His Gly Gly Lys Glu Gly Trp Ser Trp Lys Lys           
    50                  55                  60                            

tgg aac aag agc tcc ccc gcc gct gct gaa aat agt aac gtg acc aaa        240
Trp Asn Lys Ser Ser Pro Ala Ala Ala Glu Asn Ser Asn Val Thr Lys           
65                  70                  75                  80            

ttt gag ctc aga gat acc cac atc acc gcc cac gtg aca tat acg ctg        288
Phe Glu Leu Arg Asp Thr His Ile Thr Ala His Val Thr Tyr Thr Leu           
                85                  90                  95                

act aag tcg ccc gac aag aag acg tcc caa ctg gaa atc gaa tac tcc        336
Thr Lys Ser Pro Asp Lys Lys Thr Ser Gln Leu Glu Ile Glu Tyr Ser           
            100                 105                 110                   

gcc aac agt aat gag ctg ggt aag agc atg ccg att agc atg aca aat        384
Ala Asn Ser Asn Glu Leu Gly Lys Ser Met Pro Ile Ser Met Thr Asn           
        115                 120                 125                       

cac agc tac ttc tgc ttg gac aaa gac ggt gcc gaa ggt gag ggt cac        432
His Ser Tyr Phe Cys Leu Asp Lys Asp Gly Ala Glu Gly Glu Gly His           
    130                 135                 140                           

gtg acc gtc gac gac cac gag ctc tgg ctg tgt aac gat cat gtg att        480
Val Thr Val Asp Asp His Glu Leu Trp Leu Cys Asn Asp His Val Ile           
145                 150                 155                 160           

gaa cat aag gac ggt ctg caa tcg gag cct cct acg ggc gag ctt gtc        528
Glu His Lys Asp Gly Leu Gln Ser Glu Pro Pro Thr Gly Glu Leu Val           
                165                 170                 175               

cga tgc gag gca ttg ttg aag gcg ggg gcg ggt gag tca cgt gac aca        576
Arg Cys Glu Ala Leu Leu Lys Ala Gly Ala Gly Glu Ser Arg Asp Thr           
            180                 185                 190                   

ttc tcg tca ctt ggt cgc ttt tcg gac ttg tct tca ctt ggt ggg acg        624
Phe Ser Ser Leu Gly Arg Phe Ser Asp Leu Ser Ser Leu Gly Gly Thr           
        195                 200                 205                       

tcc atc tcc ctc aat gga act gtt ctc gac cac act ttt gct gtc gat        672
Ser Ile Ser Leu Asn Gly Thr Val Leu Asp His Thr Phe Ala Val Asp           
    210                 215                 220                           

aac gac agg ctt tca cgt gat gcc aag ctc gat acc cgt gat gat cca        720
Asn Asp Arg Leu Ser Arg Asp Ala Lys Leu Asp Thr Arg Asp Asp Pro           
225                 230                 235                 240           

tta gtc agc gtt gca att ttg tca cgt gga aag acc cgt ctg acc gtc        768
Leu Val Ser Val Ala Ile Leu Ser Arg Gly Lys Thr Arg Leu Thr Val           
                245                 250                 255               

aag acc acc gag ccc tgt ttc cag gtc tat acc ggc gac tac aac aat        816
Lys Thr Thr Glu Pro Cys Phe Gln Val Tyr Thr Gly Asp Tyr Asn Asn           
            260                 265                 270                   

gtg gaa ctg ctt ccc ggc gag tca cgt gac ttt ggt cga cgt tct ggg        864
Val Glu Leu Leu Pro Gly Glu Ser Arg Asp Phe Gly Arg Arg Ser Gly           
        275                 280                 285                       

gtt gcg att gag tgt tct cgc ccc acg aat gct tgg aat gtt cct ggg        912
Val Ala Ile Glu Cys Ser Arg Pro Thr Asn Ala Trp Asn Val Pro Gly           
    290                 295                 300                           

tgg cag ccg tgg gtt gat ttg ggt cag ggg gag tat ggt gct aag att        960
Trp Gln Pro Trp Val Asp Leu Gly Gln Gly Glu Tyr Gly Ala Lys Ile           
305                 310                 315                 320           

atc tat gtt ttg gag cgg gag tga                                        984
Ile Tyr Val Leu Glu Arg Glu                                               
                325                                                       


<210>  8
<211>  327
<212>  PRT
<213>  Yarrowia lipolytica

<400>  8

Met Thr Phe Cys Asn Thr Gly Ala Thr Leu Gln Ser Trp Glu Asn Ser 
1               5                   10                  15      


Ile Leu Gly Phe Glu Asn His Thr Asn Tyr Leu Asn Arg Gln Asn Pro 
            20                  25                  30          


Tyr Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr Gln Pro 
        35                  40                  45              


Asn Gly Ile Leu Leu His Gly Gly Lys Glu Gly Trp Ser Trp Lys Lys 
    50                  55                  60                  


Trp Asn Lys Ser Ser Pro Ala Ala Ala Glu Asn Ser Asn Val Thr Lys 
65                  70                  75                  80  


Phe Glu Leu Arg Asp Thr His Ile Thr Ala His Val Thr Tyr Thr Leu 
                85                  90                  95      


Thr Lys Ser Pro Asp Lys Lys Thr Ser Gln Leu Glu Ile Glu Tyr Ser 
            100                 105                 110         


Ala Asn Ser Asn Glu Leu Gly Lys Ser Met Pro Ile Ser Met Thr Asn 
        115                 120                 125             


His Ser Tyr Phe Cys Leu Asp Lys Asp Gly Ala Glu Gly Glu Gly His 
    130                 135                 140                 


Val Thr Val Asp Asp His Glu Leu Trp Leu Cys Asn Asp His Val Ile 
145                 150                 155                 160 


Glu His Lys Asp Gly Leu Gln Ser Glu Pro Pro Thr Gly Glu Leu Val 
                165                 170                 175     


Arg Cys Glu Ala Leu Leu Lys Ala Gly Ala Gly Glu Ser Arg Asp Thr 
            180                 185                 190         


Phe Ser Ser Leu Gly Arg Phe Ser Asp Leu Ser Ser Leu Gly Gly Thr 
        195                 200                 205             


Ser Ile Ser Leu Asn Gly Thr Val Leu Asp His Thr Phe Ala Val Asp 
    210                 215                 220                 


Asn Asp Arg Leu Ser Arg Asp Ala Lys Leu Asp Thr Arg Asp Asp Pro 
225                 230                 235                 240 


Leu Val Ser Val Ala Ile Leu Ser Arg Gly Lys Thr Arg Leu Thr Val 
                245                 250                 255     


Lys Thr Thr Glu Pro Cys Phe Gln Val Tyr Thr Gly Asp Tyr Asn Asn 
            260                 265                 270         


Val Glu Leu Leu Pro Gly Glu Ser Arg Asp Phe Gly Arg Arg Ser Gly 
        275                 280                 285             


Val Ala Ile Glu Cys Ser Arg Pro Thr Asn Ala Trp Asn Val Pro Gly 
    290                 295                 300                 


Trp Gln Pro Trp Val Asp Leu Gly Gln Gly Glu Tyr Gly Ala Lys Ile 
305                 310                 315                 320 


Ile Tyr Val Leu Glu Arg Glu 
                325         


<210>  9
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAL1 consensus sequence


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Replace = Ser or Thr

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Replace = Ala or Ser

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (14)..(14)
<223>  Replace = Glu or Ser

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Replace = Thr, Ser or Glu

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Replace = Arg or Thr

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Replace = His or Ala

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Replace = Gln or Arg

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  Replace = Pro, Thr or Lys

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Replace = Glu or Val

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Replace = Gln, Glu or Asn

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  Replace = Lys or Thr

<220>
<221>  VARIANT
<222>  (30)..(30)
<223>  Replace = Ser, Thr or Arg

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (34)..(34)
<223>  Replace = Pro or nothing

<220>
<221>  VARIANT
<222>  (35)..(35)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  Replace = Ser, Ala, Cys

<220>
<221>  VARIANT
<222>  (37)..(37)
<223>  Replace = Ser or Lys

<220>
<221>  VARIANT
<222>  (38)..(38)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  Replace = Leu or Val

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (67)..(67)
<223>  Replace = Gly

<220>
<221>  VARIANT
<222>  (71)..(71)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (77)..(77)
<223>  Replace = Gly or Glu

<220>
<221>  VARIANT
<222>  (80)..(80)
<223>  Replace = Ser or Lys

<220>
<221>  VARIANT
<222>  (82)..(82)
<223>  Replace = Glu or Arg

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Replace = Met or Ile

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (95)..(95)
<223>  Replace = Lys or Ile

<220>
<221>  VARIANT
<222>  (97)..(97)
<223>  Replace = Asn or His

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  Replace = Val or Cys

<220>
<221>  VARIANT
<222>  (100)..(100)
<223>  Replace = Thr, Ala or Asp

<220>
<221>  VARIANT
<222>  (101)..(101)
<223>  Replace = Ala or Pro

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Replace = Asp, Thr or Asn

<220>
<221>  VARIANT
<222>  (103)..(103)
<223>  Replace = Gln or Cys

<220>
<221>  VARIANT
<222>  (104)..(104)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (106)..(106)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (109)..(109)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (110)..(110)
<223>  Replace = Thr or Lys

<220>
<221>  VARIANT
<222>  (122)..(122)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (125)..(125)
<223>  Replace = Gly

<220>
<221>  VARIANT
<222>  (127)..(127)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  Replace = Tyr

<220>
<221>  VARIANT
<222>  (129)..(129)
<223>  Replace = Phe or Val

<220>
<221>  VARIANT
<222>  (130)..(130)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (131)..(131)
<223>  Replace = Asp or Glu

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (133)..(133)
<223>  Replace = His

<220>
<221>  VARIANT
<222>  (134)..(134)
<223>  Replace = Glu, Asp, or Gly

<220>
<221>  VARIANT
<222>  (135)..(135)
<223>  Replace = Glu or Asp

<220>
<221>  VARIANT
<222>  (136)..(136)
<223>  Replace = Leu or nothing

<220>
<221>  VARIANT
<222>  (137)..(137)
<223>  Replace = Lys or Gln

<220>
<221>  VARIANT
<222>  (138)..(138)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (139)..(139)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (143)..(143)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (169)..(169)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (171)..(171)
<223>  Replace = Leu or Met

<220>
<221>  VARIANT
<222>  (172)..(172)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (173)..(173)
<223>  Replace = Gln or Tyr

<220>
<221>  VARIANT
<222>  (175)..(175)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (176)..(176)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (177)..(177)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (178)..(178)
<223>  Replace = Met or Leu

<220>
<221>  VARIANT
<222>  (179)..(179)
<223>  Replace = Gln, Lys or Thr

<220>
<221>  VARIANT
<222>  (180)..(180)
<223>  Replace = Glu or Gln

<220>
<221>  VARIANT
<222>  (181)..(181)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (184)..(184)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (188)..(188)
<223>  Replace = Arg or Thr

<220>
<221>  VARIANT
<222>  (197)..(197)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (211)..(211)
<223>  Replace = Tyr

<220>
<221>  VARIANT
<222>  (213)..(213)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (223)..(223)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (225)..(225)
<223>  Replace = Val or Lys

<220>
<221>  VARIANT
<222>  (227)..(227)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (230)..(230)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (232)..(232)
<223>  Replace = Gln or Leu

<220>
<221>  VARIANT
<222>  (235)..(235)
<223>  Replace = Ser or Ala

<220>
<221>  VARIANT
<222>  (237)..(237)
<223>  Replace = Thr, Val or Ser

<220>
<221>  VARIANT
<222>  (239)..(239)
<223>  Replace = Lys or Ser

<220>
<221>  VARIANT
<222>  (240)..(240)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (249)..(249)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (250)..(250)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (270)..(270)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (272)..(272)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (274)..(274)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (275)..(275)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (276)..(276)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (277)..(277)
<223>  Replace = His

<220>
<221>  VARIANT
<222>  (278)..(278)
<223>  Replace = Ser or Gly

<220>
<221>  VARIANT
<222>  (280)..(280)
<223>  Replace = Asn or nothing

<220>
<221>  VARIANT
<222>  (281)..(281)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (282)..(282)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (283)..(283)
<223>  Replace = Thr, Ser or Lys

<220>
<221>  VARIANT
<222>  (284)..(284)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (285)..(285)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (287)..(287)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (295)..(295)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (296)..(296)
<223>  Replace = His or Arg

<220>
<221>  VARIANT
<222>  (299)..(299)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (300)..(300)
<223>  Replace = Lys or Glu

<220>
<221>  VARIANT
<222>  (302)..(302)
<223>  Replace = Val or Cys

<220>
<221>  VARIANT
<222>  (303)..(303)
<223>  Replace = Asp or Asn

<220>
<221>  VARIANT
<222>  (307)..(307)
<223>  Replace =  Asn, His or Thr

<220>
<221>  VARIANT
<222>  (310)..(310)
<223>  Replace =  Leu

<220>
<221>  VARIANT
<222>  (311)..(311)
<223>  Replace =  Asp or Lys

<220>
<221>  VARIANT
<222>  (313)..(313)
<223>  Replace =  Val

<220>
<221>  VARIANT
<222>  (314)..(314)
<223>  Replace =  nothing

<220>
<221>  VARIANT
<222>  (315)..(315)
<223>  Replace =  Phe

<220>
<221>  VARIANT
<222>  (316)..(316)
<223>  Replace =  Thr

<220>
<221>  VARIANT
<222>  (319)..(319)
<223>  Replace =  Lys

<220>
<221>  VARIANT
<222>  (320)..(320)
<223>  Replace =  Asp

<220>
<221>  VARIANT
<222>  (321)..(321)
<223>  Replace =  Pro

<220>
<221>  VARIANT
<222>  (322)..(322)
<223>  Replace =  Leu

<220>
<221>  VARIANT
<222>  (323)..(323)
<223>  Replace =  Thr

<220>
<221>  VARIANT
<222>  (325)..(325)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (327)..(327)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (328)..(328)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (329)..(329)
<223>  Replace = Gly, Lys or Thr

<220>
<221>  VARIANT
<222>  (332)..(332)
<223>  Replace = Asn, Asp or Glu

<220>
<221>  VARIANT
<222>  (333)..(333)
<223>  Replace = Val or Ile

<220>
<221>  VARIANT
<222>  (335)..(335)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (336)..(336)
<223>  Replace = Asp or Glu

<220>
<221>  VARIANT
<222>  (337)..(337)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (338)..(338)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (339)..(339)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (340)..(340)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (341)..(341)
<223>  Replace = Gln or Glu

<220>
<221>  VARIANT
<222>  (344)..(344)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (346)..(346)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (350)..(350)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (351)..(351)
<223>  Replace = Thr or Ser

<220>
<221>  VARIANT
<222>  (353)..(353)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (356)..(356)
<223>  Replace = Ser or Ala

<220>
<221>  VARIANT
<222>  (363)..(363)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (366)..(366)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (369)..(369)
<223>  Replace = Lys or Met

<220>
<221>  VARIANT
<222>  (370)..(370)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (372)..(372)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (373)..(373)
<223>  Replace = Asn or Lys

<220>
<221>  VARIANT
<222>  (374)..(374)
<223>  Replace = Leu or Lys

<220>
<221>  VARIANT
<222>  (377)..(377)
<223>  Replace = Lys, Leu or Glu

<220>
<221>  VARIANT
<222>  (379)..(379)
<223>  Replace = Glu or Val

<220>
<221>  VARIANT
<222>  (381)..(381)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (383)..(383)
<223>  Replace = Arg, Lys or Ile

<220>
<221>  VARIANT
<222>  (384)..(384)
<223>  Replace = Gln or Glu

<220>
<221>  VARIANT
<222>  (387)..(387)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (391)..(391)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (394)..(394)
<223>  Replace = Lys or Glu

<220>
<221>  VARIANT
<222>  (397)..(397)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (398)..(398)
<223>  Replace = Gln or Glu

<220>
<221>  VARIANT
<222>  (401)..(401)
<223>  Replace = Asn or Asp

<220>
<221>  VARIANT
<222>  (405)..(405)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (406)..(406)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (408)..(408)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (409)..(409)
<223>  Replace = Lys or Glu

<220>
<221>  VARIANT
<222>  (412)..(412)
<223>  Replace = Asp or Glu

<220>
<221>  VARIANT
<222>  (416)..(416)
<223>  Replace = Asn or Lys

<220>
<221>  VARIANT
<222>  (417)..(417)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (420)..(420)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (438)..(438)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (439)..(439)
<223>  Replace = Glu or Arg

<220>
<221>  VARIANT
<222>  (440)..(440)
<223>  Replace = Ala or Asp

<220>
<221>  VARIANT
<222>  (442)..(442)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (443)..(443)
<223>  Replace = Asn, Gln or Asp

<220>
<221>  VARIANT
<222>  (444)..(444)
<223>  Replace = Asn or Tyr

<220>
<221>  VARIANT
<222>  (447)..(447)
<223>  Replace = Asp or Gly

<220>
<221>  VARIANT
<222>  (448)..(448)
<223>  Replace = Ala or Thr

<220>
<221>  VARIANT
<222>  (451)..(451)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (455)..(455)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (456)..(456)
<223>  Replace = Asp or Arg

<220>
<221>  VARIANT
<222>  (457)..(457)
<223>  Replace = Met or Leu

<220>
<221>  VARIANT
<222>  (458)..(458)
<223>  Replace = Asp, Glu or Asn

<220>
<221>  VARIANT
<222>  (459)..(459)
<223>  Replace = Gly or Lys

<220>
<221>  VARIANT
<222>  (460)..(460)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (461)..(461)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (462)..(462)
<223>  Replace = Tyr or Ala

<220>
<221>  VARIANT
<222>  (464)..(464)
<223>  Replace = Ser or Asn

<220>
<221>  VARIANT
<222>  (466)..(466)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (467)..(467)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (473)..(473)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (476)..(476)
<223>  Replace = Cys

<220>
<221>  VARIANT
<222>  (478)..(478)
<223>  Replace = Ile or Val

<220>
<221>  VARIANT
<222>  (480)..(480)
<223>  Replace = Leu, Cys or Phe

<220>
<221>  VARIANT
<222>  (481)..(481)
<223>  Replace = Lys, Ser or nothing

<220>
<221>  VARIANT
<222>  (482)..(482)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (483)..(483)
<223>  Replace = nothing

<400>  9

Met Asn Val Ala Lys Leu Gly Leu Glu Lys Ile Tyr Ala Asp Pro Ala 
1               5                   10                  15      


Lys Gln Ser Ala Arg Tyr Asp Asp Ile Thr Ala Arg Phe Lys Gln Leu 
            20                  25                  30          


Tyr Ala Gly Gln Thr Pro Thr Ile Val Arg Ser Pro Gly Arg Val Asn 
        35                  40                  45              


Leu Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met 
    50                  55                  60                  


Ala Ile Glu Asp Asp Val Val Met Ala Phe Cys Val Ala Gly Asp Thr 
65                  70                  75                  80  


Ile Asp Val Ala Asn Leu Glu Asp Asp Lys Phe Pro Ala Glu Ser Tyr 
                85                  90                  95      


Asp Ile Pro Ser Gly Glu Glu Val Leu Glu Ile Asp Thr Gln Lys Thr 
            100                 105                 110         


Val Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Ala Arg Arg His 
        115                 120                 125             


Leu Asn Leu Ser Gly Lys Ser Val Arg Asp Val Gly Ile Lys Val Leu 
    130                 135                 140                 


Val His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala 
145                 150                 155                 160 


Phe Val Val Cys Ser Thr Leu Val Ser Leu Ile Ala Cys Gly Gly Gly 
                165                 170                 175     


Ala Glu Asp Ser Ile Ser Arg Asp Leu Leu Thr Lys Leu Ser Ile Thr 
            180                 185                 190         


Cys Glu Gln Leu Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala 
        195                 200                 205             


Ser Ile Phe Gly Gln Leu Asn His Thr Leu Leu Val Asp Phe Ser Pro 
    210                 215                 220                 


Thr Leu Lys Ala Thr Ser Phe Lys Phe Pro Glu Thr Arg Pro Glu Thr 
225                 230                 235                 240 


Val Phe Leu Val Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr 
                245                 250                 255     


Ala Pro Arg Asn Tyr Asn Leu Arg Val Val Glu Ile Thr Ile Ala Ala 
            260                 265                 270         


Asn Val Ile Ala Lys Lys Leu Lys Gly Leu Gln Leu Val Gln Asn Gly 
        275                 280                 285             


Asn Leu Asn Lys Gly Thr Phe Asn Gly Leu Met Gln Ala Ala Gly Thr 
    290                 295                 300                 


Asp Leu Ala Gly Leu Glu Gly Leu Ile Asp Glu His Leu Pro Arg Glu 
305                 310                 315                 320 


Gly Val Ser Leu Glu Glu Ala Ser Glu Leu Leu Gly Ala Ser Val Ala 
                325                 330                 335     


Glu Val Lys Glu Ser Tyr Phe Val Phe His Val Glu Phe Asp Lys Leu 
            340                 345                 350         


Gln Leu Tyr Lys Arg Ala Lys His Val Val Thr Glu Ala Ile Arg Val 
        355                 360                 365             


Leu Glu Phe Gln Ser Met Leu Ser Ser Gly Thr Ser Asp Pro Ser Lys 
    370                 375                 380                 


Leu Gly Asn Leu Met Asn Leu Ser Gln Gln Ser Cys Arg Asp Leu Phe 
385                 390                 395                 400 


Glu Cys Ser Cys Asp Ala Ile Asp Arg Val Cys Lys Ile Val Leu Ala 
                405                 410                 415     


Ser Gly Gly Leu Gly Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser 
            420                 425                 430         


Thr Val His Leu Val Glu Lys Glu Lys Leu Ser Ser Val Ile Glu Ser 
        435                 440                 445             


Leu Lys Lys Glu Tyr Tyr Ser Gly Ile Gly Glu Val Asp Phe Asp Thr 
    450                 455                 460                 


Ala Met Val Val Thr Val Pro Gly Glu Gly Thr Ala Phe Val Asp Ile 
465                 470                 475                 480 


Gln Leu His 
            


<210>  10
<211>  476
<212>  PRT
<213>  Yarrowia galli

<400>  10

Met Ser Ile Ser Thr Leu Pro Leu Ser Lys Ile Tyr Ala Asp Pro Ser 
1               5                   10                  15      


Lys His Gln Thr Arg Tyr Glu Glu Leu Thr Lys Arg Phe Ser Gln Leu 
            20                  25                  30          


Tyr Pro Gly Ser Thr Ala Thr Leu Val Arg Ser Pro Gly Arg Val Asn 
        35                  40                  45              


Leu Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met 
    50                  55                  60                  


Ala Ile Glu Asp Asp Val Ile Met Ala Phe Ser Val Gly Gly Asp Ser 
65                  70                  75                  80  


Ile Glu Ile Ala Asn Met Glu Asp Glu Lys Phe Pro Ala Glu Lys Tyr 
                85                  90                  95      


Asp Ile Pro Thr Ala Thr Glu Val Leu Thr Ile Asp Gln Thr Lys Thr 
            100                 105                 110         


Val Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Gly Arg Lys Tyr 
        115                 120                 125             


Leu Arg Glu Ser Gly Glu Glu Val Lys Pro Val Gly Ile Lys Val Leu 
    130                 135                 140                 


Val His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala 
145                 150                 155                 160 


Phe Val Val Cys Ser Thr Leu Val Thr Leu Leu Ser Gln Gly Met Lys 
                165                 170                 175     


Glu Val Ser Arg Asp Leu Leu Thr Lys Leu Ser Ile Thr Cys Glu Gln 
            180                 185                 190         


Leu Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile Tyr 
        195                 200                 205             


Gly Gln Leu Asn His Thr Leu Leu Val Asp Phe Ala Pro Thr Leu Lys 
    210                 215                 220                 


Ala Thr Pro Phe Gln Phe Pro Ala Thr Thr Pro Lys Thr Val Phe Leu 
225                 230                 235                 240 


Val Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr Ala Pro Arg 
                245                 250                 255     


Asn Tyr Asn Leu Arg Val Val Glu Ile Thr Leu Ala Val Asn Val Leu 
            260                 265                 270         


Leu Lys Ser Leu Asn Gly Val Ser Ile Pro Gln Asn Gly Asn Leu Asn 
        275                 280                 285             


Lys Gly Thr Leu His Gly Leu Met Lys Ala Val Gly Thr Asp Leu Asn 
    290                 295                 300                 


Gly Leu Leu Asp Leu Val Glu Thr Leu Pro Lys Glu Pro Val Thr Leu 
305                 310                 315                 320 


Gln Glu Val Ala Lys Leu Leu Asp Ile Ser Glu Glu Asp Val Lys Lys 
                325                 330                 335     


Gln Tyr Phe Ile Phe Pro Val Glu Phe Asp Thr Leu Ala Leu Tyr Ala 
            340                 345                 350         


Arg Ala Lys His Val Val Thr Glu Ala Ile Arg Val Leu Glu Phe Gln 
        355                 360                 365             


Ser Leu Leu Ser Lys Gly Glu Ser Asp Pro Lys Glu Leu Gly Lys Leu 
    370                 375                 380                 


Met Asn Glu Ser Gln Lys Ser Cys Asp Gln Leu Phe Asn Cys Ser Cys 
385                 390                 395                 400 


Pro Ala Ile Glu Lys Val Cys Glu Ile Val Leu Lys Ser Gly Gly Phe 
                405                 410                 415     


Gly Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His Leu 
            420                 425                 430         


Val Glu Glu Ala Lys Leu Asn Asn Val Ile Gly Ala Leu Lys Lys Glu 
        435                 440                 445             


Tyr Tyr Ser Asp Met Glu Gly Val Asp Tyr Asp Ser Ala Met Val Val 
    450                 455                 460                 


Thr Val Pro Gly Glu Gly Cys Ala Ile Val Asp Leu 
465                 470                 475     


<210>  11
<211>  476
<212>  PRT
<213>  Yarrowia yakushimensis

<400>  11

Met Ser Ile Ala Thr Leu Pro Leu Ser Lys Ile Tyr Ala Asp Pro Ala 
1               5                   10                  15      


Arg His Gln Ala Arg Tyr Glu Gln Leu Thr Lys Arg Phe Thr Glu Leu 
            20                  25                  30          


Tyr Gly Ala Lys Pro Thr Val Val Arg Ser Pro Gly Arg Val Asn Leu 
        35                  40                  45              


Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met Ala 
    50                  55                  60                  


Ile Glu Asp Asp Val Ile Met Ala Phe Ser Val Gly Gly Asp Ser Ile 
65                  70                  75                  80  


Glu Ile Ala Asn Ile Glu Asp Glu Lys Phe Pro Ala Glu Lys Tyr Asp 
                85                  90                  95      


Ile Pro Ala Pro Asn Glu Val Leu Thr Ile Asp Gln Lys Lys Thr Val 
            100                 105                 110         


Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Ala Arg Lys His Phe 
        115                 120                 125             


Arg Asp Ser Gly Asp Asp Val Lys Pro Val Gly Ile Lys Ile Leu Val 
    130                 135                 140                 


His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala Phe 
145                 150                 155                 160 


Val Val Cys Ser Thr Leu Val Thr Leu Leu Ser Gln Gly Leu Thr Glu 
                165                 170                 175     


Val Ser Arg Asp Leu Leu Thr Thr Leu Ser Ile Thr Cys Glu Gln Leu 
            180                 185                 190         


Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile Tyr Gly 
        195                 200                 205             


Gln Leu Asn His Thr Leu Leu Val Asp Phe Ala Pro Val Leu Lys Ala 
    210                 215                 220                 


Thr Pro Phe Leu Phe Pro Ser Thr Val Pro Lys Thr Val Phe Leu Val 
225                 230                 235                 240 


Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr Ala Pro Arg Asn 
                245                 250                 255     


Tyr Asn Leu Arg Val Val Glu Ile Thr Leu Ala Val Asn Val Leu Leu 
            260                 265                 270         


Lys Gly Leu Lys Asp Val Thr Ile Pro Gln Asn Gly Asn Leu Asn Lys 
        275                 280                 285             


Gly Thr Leu Arg Gly Leu Met Glu Ala Val Gly Thr Asp Leu His Gly 
    290                 295                 300                 


Leu Leu Asp Leu Val Glu Thr Leu Pro Lys Glu Pro Val Thr Leu Gln 
305                 310                 315                 320 


Glu Val Ala Lys Leu Leu Asp Ile Ser Glu Glu Asp Val Lys Lys Gln 
                325                 330                 335     


Tyr Phe Ile Phe Pro Val Glu Phe Asp Thr Leu Ala Leu Tyr Ser Arg 
            340                 345                 350         


Ala Lys His Val Val Thr Glu Ala Leu Arg Val Leu Glu Phe Gln Lys 
        355                 360                 365             


Lys Leu Ser Leu Gly Glu Ser Asp Pro Lys Gln Leu Gly Lys Leu Met 
    370                 375                 380                 


Asn Glu Ser Gln Lys Ser Cys Asp Gln Leu Phe Asn Cys Ser Cys Pro 
385                 390                 395                 400 


Ala Ile Glu Glu Val Cys Glu Ile Val Leu Lys Ser Gly Gly Leu Gly 
                405                 410                 415     


Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His Leu Val 
            420                 425                 430         


Glu Glu Ala Lys Leu Gln Asn Val Ile Asp Ala Leu Lys Lys Glu Tyr 
        435                 440                 445             


Tyr Ser Asp Met Glu Gly Val Asp Tyr Asp Asn Ala Met Val Val Thr 
    450                 455                 460                 


Val Pro Gly Glu Gly Cys Ala Val Val Asp Cys Lys 
465                 470                 475     


<210>  12
<211>  478
<212>  PRT
<213>  Yarrowia alimentaria

<400>  12

Met Asn Val Ala Lys Leu Gly Leu Glu Lys Ile Tyr Ala Asp Pro Ala 
1               5                   10                  15      


Lys Gln Ser Ala Arg Tyr Asp Asp Ile Thr Ala Arg Phe Lys Gln Leu 
            20                  25                  30          


Tyr Gly Gln Thr Pro Thr Ile Val Arg Ser Pro Gly Arg Val Asn Leu 
        35                  40                  45              


Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met Ala 
    50                  55                  60                  


Ile Glu Asp Asp Val Val Met Ala Phe Cys Val Ala Gly Asp Thr Ile 
65                  70                  75                  80  


Asp Val Ala Asn Leu Glu Asp Asp Lys Phe Pro Ala Glu Ser Tyr Asp 
                85                  90                  95      


Ile Pro Ser Gly Glu Glu Val Leu Glu Ile Asp Thr Gln Lys Thr Val 
            100                 105                 110         


Trp Thr Asn Tyr Phe Lys Cys Gly His Thr Val Ala Arg Arg His Leu 
        115                 120                 125             


Asn Leu Ser Gly Lys Ser Arg Asp Val Gly Ile Lys Val Leu Val His 
    130                 135                 140                 


Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala Phe Val 
145                 150                 155                 160 


Val Cys Ser Thr Leu Val Ser Leu Ile Ala Cys Gly Gly Gly Ala Glu 
                165                 170                 175     


Asp Ser Ile Ser Arg Asp Leu Leu Thr Lys Leu Ser Ile Thr Cys Glu 
            180                 185                 190         


Gln Leu Ile Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile 
        195                 200                 205             


Phe Gly Gln Leu Asn His Thr Leu Leu Val Asp Phe Ser Pro Thr Leu 
    210                 215                 220                 


Lys Ala Thr Ser Phe Lys Phe Pro Glu Thr Arg Pro Glu Thr Val Phe 
225                 230                 235                 240 


Leu Val Ala Asn Thr Leu Val Lys Ser Asn Lys His Glu Thr Ala Pro 
                245                 250                 255     


Arg Asn Tyr Asn Leu Arg Val Val Glu Ile Thr Ile Ala Ala Asn Val 
            260                 265                 270         


Ile Ala Lys Lys Leu Gly Leu Gln Leu Val Gln Asn Gly Asn Leu Asn 
        275                 280                 285             


Lys Gly Thr Phe Asn Gly Leu Met Gln Ala Ala Gly Thr Asp Leu Ala 
    290                 295                 300                 


Gly Leu Glu Gly Leu Ile Asp Glu His Leu Pro Arg Glu Gly Val Ser 
305                 310                 315                 320 


Leu Glu Glu Ala Ser Glu Leu Leu Gly Ala Ser Val Ala Glu Val Lys 
                325                 330                 335     


Glu Ser Tyr Phe Val Phe His Val Glu Phe Asp Lys Leu Gln Leu Tyr 
            340                 345                 350         


Lys Arg Ala Lys His Val Val Thr Glu Ala Ile Arg Val Leu Glu Phe 
        355                 360                 365             


Gln Ser Met Leu Ser Ser Gly Thr Ser Asp Pro Ser Lys Leu Gly Asn 
    370                 375                 380                 


Leu Met Asn Leu Ser Gln Gln Ser Cys Arg Asp Leu Phe Glu Cys Ser 
385                 390                 395                 400 


Cys Asp Ala Ile Asp Arg Val Cys Lys Ile Val Leu Ala Ser Gly Gly 
                405                 410                 415     


Leu Gly Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His 
            420                 425                 430         


Leu Val Glu Lys Glu Lys Leu Ser Ser Val Ile Glu Ser Leu Lys Lys 
        435                 440                 445             


Glu Tyr Tyr Ser Gly Ile Gly Glu Val Asp Phe Asp Thr Ala Met Val 
    450                 455                 460                 


Val Thr Val Pro Gly Glu Gly Thr Ala Phe Val Asp Ile Gln 
465                 470                 475             


<210>  13
<211>  477
<212>  PRT
<213>  Yarrowia phangngensis

<400>  13

Met Ser Ile Thr Lys Leu Pro Leu Glu Lys Ile Tyr Ser Ser Pro Glu 
1               5                   10                  15      


Thr Ala Arg Lys Arg Tyr Val Asn Ile Val Thr Arg Phe Arg Glu Leu 
            20                  25                  30          


Tyr Gly Cys Thr Pro Thr Leu Val Arg Ala Pro Gly Arg Val Asn Leu 
        35                  40                  45              


Ile Gly Glu His Ile Asp Tyr Ser His Phe Ser Val Met Pro Met Ala 
    50                  55                  60                  


Ile Gly Asp Asp Val Ile Met Ala Phe Cys Val Glu Gly Asp Lys Ile 
65                  70                  75                  80  


Arg Ile Ala Asn Leu Glu Asp Glu Lys Phe Pro Ser Glu Ile Tyr His 
                85                  90                  95      


Cys Pro Asp Ala Asp Cys Glu Leu Glu Ile Asp Gln Thr Lys Thr Val 
            100                 105                 110         


Trp Thr Asn Tyr Phe Lys Cys Gly Phe Thr Val Gly Arg Lys Tyr Val 
        115                 120                 125             


Arg Glu Thr His Gly Ser Leu Gln Pro Leu Gly Ile Lys Ile Leu Val 
    130                 135                 140                 


His Gly Thr Val Pro Asp Ala Cys Gly Leu Ser Ser Ser Ala Ala Phe 
145                 150                 155                 160 


Val Val Cys Ser Thr Leu Val Thr Leu Met Ala Tyr Gly Glu Thr Gln 
                165                 170                 175     


Val Ser Arg Glu Leu Leu Thr Lys Leu Ser Ile Thr Cys Glu Gln Leu 
            180                 185                 190         


Val Gly Val Asn Thr Gly Gly Met Asp Gln Ala Ala Ser Ile Tyr Gly 
        195                 200                 205             


Val Leu Asn His Thr Leu Leu Val Asp Phe Ala Pro Lys Leu Arg Ala 
    210                 215                 220                 


Thr Pro Phe Leu Phe Pro Glu Thr Ser Pro Ser Ala Val Phe Leu Val 
225                 230                 235                 240 


Ala Asn Thr Leu Ile Arg Ser Asn Lys His Glu Thr Ala Pro Arg Asn 
                245                 250                 255     


Tyr Asn Leu Arg Val Val Glu Ile Thr Leu Ala Val Asn Ile Leu Ala 
            260                 265                 270         


His Lys Leu Gly Leu Lys Ile Pro Gln Asp Gly Asn Leu Asn Lys Gly 
        275                 280                 285             


Thr Phe His Gly Leu Leu Glu Ala Cys Asn Thr Asp Leu Thr Gly Leu 
    290                 295                 300                 


Leu Lys Leu Val Asp Phe His Leu Pro Arg Asp Pro Leu Thr Leu Glu 
305                 310                 315                 320 


Glu Val Ser Thr Leu Leu Glu Ile Ser Glu Asp Glu Ile Arg Lys Glu 
                325                 330                 335     


Tyr Phe Val Phe Pro Val Glu Phe Lys Ser Leu Ala Leu Tyr Ser Arg 
            340                 345                 350         


Ala Lys His Val Val Ser Glu Ala Ile Arg Val Met Lys Phe Arg Lys 
        355                 360                 365             


Lys Leu Ser Glu Gly Val Ser Asn Pro Ile Glu Leu Gly Lys Leu Met 
    370                 375                 380                 


Asn Glu Ser Gln Glu Ser Cys Arg Glu Leu Phe Asp Cys Ser Cys Pro 
385                 390                 395                 400 


Glu Ile Asp Arg Val Cys Glu Ile Val Leu Lys Ala Gly Gly Phe Gly 
                405                 410                 415     


Ser Arg Leu Thr Gly Ala Gly Trp Gly Gly Ser Thr Val His Leu Val 
            420                 425                 430         


Gln Arg Asp Lys Val Asp Tyr Val Ile Asp Thr Leu Lys Ser Glu Tyr 
        435                 440                 445             


Tyr Glu Arg Leu Asn Lys Lys Ala Asp Ser Ala Ile Leu Val Thr Val 
    450                 455                 460                 


Pro Gly Gln Gly Cys Ala Ile Val Asp Phe Ser Leu His 
465                 470                 475         


<210>  14
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAL7 consensus sequence


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Replace = Val or Glu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Replace = Ser or Phe

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  Replace = Thr or Phe

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  Replace = Thr or Asp

<220>
<221>  VARIANT
<222>  (49)..(49)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (56)..(56)
<223>  Replace = Cys

<220>
<221>  VARIANT
<222>  (58)..(58)
<223>  Replace = Ala or Glu

<220>
<221>  VARIANT
<222>  (60)..(60)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (64)..(64)
<223>  Replace = Met or Lys

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (70)..(70)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (72)..(72)
<223>  Replace = Tyr

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (84)..(84)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Replace = Ala or Asp

<220>
<221>  VARIANT
<222>  (88)..(88)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (90)..(90)
<223>  Replace = Lys or Glu

<220>
<221>  VARIANT
<222>  (91)..(91)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (92)..(92)
<223>  Replace = Val, Ile or Leu

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Replace = Glu or Arg

<220>
<221>  VARIANT
<222>  (95)..(95)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (96)..(96)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (97)..(97)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  Replace = Gln, Ser or Asn

<220>
<221>  VARIANT
<222>  (99)..(99)
<223>  Replace = Lys or Glu

<220>
<221>  VARIANT
<222>  (100)..(100)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (101)..(101)
<223>  Replace = Glu or Lys

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Replace = His, Glu, Gln or Asn

<220>
<221>  VARIANT
<222>  (103)..(103)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (104)..(104)
<223>  Replace = Glu, Lys or Gly

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Replace = Ser, Pro or Phe

<220>
<221>  VARIANT
<222>  (106)..(106)
<223>  Replace = Thr, Ser or His

<220>
<221>  VARIANT
<222>  (107)..(107)
<223>  Replace = Ala or Glu

<220>
<221>  VARIANT
<222>  (108)..(108)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (109)..(109)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (110)..(110)
<223>  Replace = Leu, Arg or Ser

<220>
<221>  VARIANT
<222>  (111)..(111)
<223>  Replace = Leu or Asn

<220>
<221>  VARIANT
<222>  (114)..(114)
<223>  Replace = Gln or Asn

<220>
<221>  VARIANT
<222>  (115)..(115)
<223>  Replace = Lys or Arg

<220>
<221>  VARIANT
<222>  (116)..(116)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (117)..(117)
<223>  Replace = Asp or Glu

<220>
<221>  VARIANT
<222>  (118)..(118)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (137)..(137)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (140)..(140)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (141)..(141)
<223>  Replace = Glu or Thr

<220>
<221>  VARIANT
<222>  (144)..(144)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (145)..(145)
<223>  Replace = Lys, Arg or Ala

<220>
<221>  VARIANT
<222>  (147)..(147)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (148)..(148)
<223>  Replace = Glu, Ala or Asn

<220>
<221>  VARIANT
<222>  (151)..(151)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (152)..(152)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (153)..(153)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (155)..(155)
<223>  Replace = Thr or Glu

<220>
<221>  VARIANT
<222>  (159)..(159)
<223>  Replace = Ser or Arg

<220>
<221>  VARIANT
<222>  (160)..(160)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (161)..(161)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (173)..(173)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (174)..(174)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (184)..(184)
<223>  Replace = Cys

<220>
<221>  VARIANT
<222>  (191)..(191)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (194)..(194)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (198)..(198)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (200)..(200)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (201)..(201)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (202)..(202)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (204)..(204)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (205)..(205)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (208)..(208)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (209)..(209)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (210)..(210)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (211)..(211)
<223>  Replace = Asn or Lys

<220>
<221>  VARIANT
<222>  (219)..(219)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (220)..(220)
<223>  Replace = Asn, His, Gln or Glu

<220>
<221>  VARIANT
<222>  (223)..(223)
<223>  Replace = Met

<220>
<221>  VARIANT
<222>  (224)..(224)
<223>  Replace = Ser or Gln

<220>
<221>  VARIANT
<222>  (226)..(226)
<223>  Replace = Asn or Glu

<220>
<221>  VARIANT
<222>  (229)..(229)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (232)..(232)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (234)..(234)
<223>  Replace = Ala or Glu

<220>
<221>  VARIANT
<222>  (253)..(253)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (256)..(256)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (258)..(258)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (259)..(259)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (261)..(261)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (262)..(262)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (265)..(265)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (266)..(266)
<223>  Replace = Gln or Asn

<220>
<221>  VARIANT
<222>  (267)..(267)
<223>  Replace = His or Glu

<220>
<221>  VARIANT
<222>  (269)..(269)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (270)..(270)
<223>  Replace = Gln, Glu or Ser

<220>
<221>  VARIANT
<222>  (271)..(271)
<223>  Replace = His

<220>
<221>  VARIANT
<222>  (275)..(275)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (279)..(279)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (281)..(281)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (285)..(285)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (288)..(288)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (304)..(304)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (305)..(305)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (306)..(306)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (308)..(308)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (345)..(345)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (347)..(347)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (350)..(350)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (353)..(353)
<223>  Replace = Tyr, Phe or Arg

<220>
<221>  VARIANT
<222>  (354)..(354)
<223>  Replace = Asp or Glu

<400>  14

Met Thr Leu Ser Pro Ala Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Lys Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Val Lys Asp Ser Lys Pro Glu Tyr Asp 
        35                  40                  45              


Asp Lys Cys Tyr Leu Cys Pro Arg Asn Lys Arg Ile Asn Gly Glu Leu 
    50                  55                  60                  


Asn Pro Asp Tyr Gln Ser Thr Phe Val Phe Glu Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Pro Lys Thr Glu Glu Ile Glu Ser Leu Met Lys Glu Glu Lys 
                85                  90                  95      


Ser Glu Gln Lys Thr Ser Ser Ser Leu Ala Gly Ile Glu Ala Asp Leu 
            100                 105                 110         


Leu Arg Gln Asp Pro Thr Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser 
        115                 120                 125             


Pro Lys His Asn Val Thr Leu Ala Thr Met Thr Val Pro Gln Ile Glu 
    130                 135                 140                 


Gln Val Ile Tyr Thr Trp Gln Asp Met Tyr Arg Glu Ala Gln Thr Arg 
145                 150                 155                 160 


Gln Ile Lys Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly 
                165                 170                 175     


Cys Ser Asn Pro His Ala His Gly Gln Val Trp Asn Thr Ser Val Val 
            180                 185                 190         


Pro Glu Glu Pro Gln Met Glu Tyr Asn Ala Leu Arg Glu Tyr Lys Asp 
        195                 200                 205             


Ser Lys Ser Arg Asp Leu Leu Val Asp Tyr Ala Ala Leu Glu Leu Glu 
    210                 215                 220                 


Lys Lys Glu Arg Val Val Tyr Glu Asn Ser Ser Phe Leu Val Val Val 
225                 230                 235                 240 


Pro Tyr Trp Ala Val Trp Pro Phe Glu Thr Met Ile Phe Ser Lys Gln 
                245                 250                 255     


Arg Ile Ala Ser Val Ile Lys Leu Thr Lys Pro Gln Val Ala Asp Leu 
            260                 265                 270         


Ala Asp Ala Ile Ser Val Leu Thr Arg Lys Tyr Asp Lys Leu Phe Asn 
        275                 280                 285             


Thr Ser Phe Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Glu 
    290                 295                 300                 


Pro Asn Cys Glu Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu 
305                 310                 315                 320 


Leu Arg Ser Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu 
                325                 330                 335     


Gly Met Pro Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Gln Arg Leu 
            340                 345                 350         


Lys Gly Leu 
        355 


<210>  15
<211>  353
<212>  PRT
<213>  Yarrowia galli

<400>  15

Met Thr Leu Val Ala Ser Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Gln Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Thr Lys Asp Thr Lys Pro Glu Tyr Asp 
        35                  40                  45              


Asp Lys Cys Tyr Leu Cys Pro Arg Asn Ala Arg Ile Asn Gly Glu Met 
    50                  55                  60                  


Asn Pro Asp Tyr Lys Asp Thr Phe Val Phe Glu Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Glu Lys Thr Ala Glu Val Glu Lys Val Ile Glu Glu Ala Gln 
                85                  90                  95      


Lys Lys Glu Glu Ser Lys Pro Ser Glu Leu Glu Leu Asp Leu Leu Arg 
            100                 105                 110         


Lys Asp Asp Val Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser Pro Lys 
        115                 120                 125             


His Asn Val Thr Leu Ala Thr Met Thr Val Glu Gln Ile Gln Arg Val 
    130                 135                 140                 


Ile Glu Thr Trp Gln Lys Met Tyr Thr Glu Ala Gln Ser Arg Gly Ile 
145                 150                 155                 160 


Lys Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly Cys Ser 
                165                 170                 175     


Asn Pro His Ala His Gly Gln Val Trp Asn Thr Ser Val Val Pro Glu 
            180                 185                 190         


Glu Pro Gln Met Glu Phe Asp Ser Leu Lys Lys Tyr Lys Asp Glu Lys 
        195                 200                 205             


Lys Arg Asp Leu Leu Val Asp Tyr Val His Leu Glu Met Ser Lys Asn 
    210                 215                 220                 


Glu Arg Ile Val Tyr Gln Asn Ala Ser Phe Leu Val Val Val Pro Tyr 
225                 230                 235                 240 


Trp Ala Val Trp Pro Phe Glu Thr Met Ile Val Ser Lys Glu Arg Val 
                245                 250                 255     


Ser Ser Ile Lys Lys Leu Thr Gln Glu Gln Val Glu His Leu Ala Asp 
            260                 265                 270         


Ala Ile Ser Val Leu Thr Lys Lys Tyr Asp Lys Leu Phe Asn Thr Ser 
        275                 280                 285             


Phe Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Val Glu Asp 
    290                 295                 300                 


Cys Pro Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu Leu Arg 
305                 310                 315                 320 


Ser Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu Gly Met 
                325                 330                 335     


Pro Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Lys Arg Leu Phe Asp 
            340                 345                 350         


Leu 
    


<210>  16
<211>  352
<212>  PRT
<213>  Yarrowia yakushimensis

<400>  16

Met Thr Leu Val Ala Ser Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Gln Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Thr Lys Asp Thr Lys Pro Glu Tyr Asp 
        35                  40                  45              


Glu Lys Cys Tyr Leu Cys Pro Arg Asn Ala Arg Ile Asn Gly Glu Met 
    50                  55                  60                  


Asn Pro Asp Tyr Lys Asp Thr Phe Val Phe Glu Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Glu Lys Thr Ala Glu Val Glu Lys Val Ile Glu Glu Ala Ser 
                85                  90                  95      


Lys Lys Gln Ser Glu Ser Ser Glu Leu Glu Arg Leu Leu Leu Gln Lys 
            100                 105                 110         


Glu Asp Val Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser Pro Lys His 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Met Thr Val Glu Gln Ile Glu Lys Val Ile 
    130                 135                 140                 


Ala Thr Trp Gln Lys Met Tyr Thr Glu Ala Gln Ser Arg Gly Ile Lys 
145                 150                 155                 160 


Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly Cys Ser Asn 
                165                 170                 175     


Pro His Ala His Gly Gln Val Trp Asn Thr Ser Val Val Pro Glu Glu 
            180                 185                 190         


Pro Gln Met Glu Tyr Asp Ser Leu Lys Lys Tyr Lys Glu Glu Asn Lys 
        195                 200                 205             


Arg Asp Leu Leu Val Asp Tyr Val Gln Leu Glu Leu Ser Lys Asn Glu 
    210                 215                 220                 


Arg Ile Val Tyr Gln Asn Ala Ser Phe Leu Val Val Val Pro Tyr Trp 
225                 230                 235                 240 


Ala Val Trp Pro Phe Glu Thr Met Ile Val Ser Lys Glu Arg Val Ser 
                245                 250                 255     


Ser Ile Lys Lys Leu Thr Lys Glu Gln Ala Glu His Leu Ala Asp Ala 
            260                 265                 270         


Ile Ser Val Leu Thr Lys Lys Tyr Asp Lys Leu Phe Asn Thr Ser Phe 
        275                 280                 285             


Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Val Glu Asp Cys 
    290                 295                 300                 


Pro Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu Leu Arg Ser 
305                 310                 315                 320 


Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu Gly Met Pro 
                325                 330                 335     


Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Lys Arg Leu Tyr Glu Leu 
            340                 345                 350         


<210>  17
<211>  352
<212>  PRT
<213>  Yarrowia phangngensis

<400>  17

Met Thr Leu Glu Ala Phe Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Gln Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Phe Lys Asp Asp Lys Pro Glu Tyr Asp 
        35                  40                  45              


Glu Lys Cys Tyr Leu Cys Pro Cys Asn Glu Arg Val Asn Gly Glu Lys 
    50                  55                  60                  


Asn Pro Asp Tyr Gln Asp Thr Tyr Val Phe Asn Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Glu Arg Thr Asp Glu Val Glu Glu Leu Leu Arg Glu Ser Asn 
                85                  90                  95      


Glu Thr Asn Ser Gly Phe His Glu Ile Glu Ser Asn Leu Leu Asn Arg 
            100                 105                 110         


Glu Glu Val Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser Pro Lys His 
        115                 120                 125             


Asn Val Thr Leu Ala Ser Met Thr Pro Thr Gln Ile Glu Ala Val Val 
    130                 135                 140                 


Asn Thr Trp Arg Lys Ile Tyr Glu Glu Ala Gln Arg Lys Gly Ile Lys 
145                 150                 155                 160 


Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ser Ala Met Gly Cys Ser Asn 
                165                 170                 175     


Pro His Ala His Cys Gln Val Trp Asn Thr Ser Val Val Pro Asp Glu 
            180                 185                 190         


Pro Gln Leu Glu Phe Asn Ser Leu Lys Lys Tyr Lys Asp Glu Lys Lys 
        195                 200                 205             


Arg Asp Leu Leu Val Asp Tyr Val Gly Leu Glu Leu Gln Lys Glu Glu 
    210                 215                 220                 


Arg Ile Val Tyr Gln Asn Glu Ser Phe Leu Val Val Val Pro Tyr Trp 
225                 230                 235                 240 


Ala Val Trp Pro Phe Glu Thr Met Ile Val Ser Lys Glu Arg Ile Ser 
                245                 250                 255     


Ser Ile Lys Lys Leu Ser Asn His Gln Val Ser Asp Leu Ala Asp Ser 
            260                 265                 270         


Ile Ser Val Leu Thr Arg Lys Tyr Asp Asp Leu Phe Lys Thr Ser Phe 
        275                 280                 285             


Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Val Glu Asp Cys 
    290                 295                 300                 


Pro Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu Leu Arg Ser 
305                 310                 315                 320 


Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu Gly Met Pro 
                325                 330                 335     


Gln Arg Asp Ile Thr Thr Glu Glu Ala Ala Lys Arg Leu Arg Asp Leu 
            340                 345                 350         


<210>  18
<211>  355
<212>  PRT
<213>  Yarrowia alimentaria

<400>  18

Met Thr Leu Ser Pro Ala Asp Ile Pro His Arg Arg Tyr Asn Pro Leu 
1               5                   10                  15      


Arg Lys Ser Trp Val Leu Val Ser Pro His Arg Ala Lys Arg Pro Trp 
            20                  25                  30          


Gln Gly Gln Gln Glu Glu Pro Val Lys Asp Ser Lys Pro Glu Tyr Asp 
        35                  40                  45              


Asp Lys Cys Tyr Leu Cys Pro Arg Asn Lys Arg Ile Asn Gly Glu Leu 
    50                  55                  60                  


Asn Pro Asp Tyr Gln Ser Thr Phe Val Phe Glu Asn Asp Phe Ser Ala 
65                  70                  75                  80  


Val Phe Pro Lys Thr Glu Glu Ile Glu Ser Leu Met Lys Glu Glu Lys 
                85                  90                  95      


Ser Glu Gln Lys Thr Ser Ser Ser Leu Ala Gly Ile Glu Ala Asp Leu 
            100                 105                 110         


Leu Arg Gln Asp Pro Thr Phe Gly Tyr Cys Gln Val Ile Cys Phe Ser 
        115                 120                 125             


Pro Lys His Asn Val Thr Leu Ala Thr Met Thr Val Pro Gln Ile Glu 
    130                 135                 140                 


Gln Val Ile Tyr Thr Trp Gln Asp Met Tyr Arg Glu Ala Gln Thr Arg 
145                 150                 155                 160 


Gln Ile Lys Tyr Ser Leu Ile Phe Glu Asn Lys Gly Ala Thr Met Gly 
                165                 170                 175     


Cys Ser Asn Pro His Ala His Gly Gln Val Trp Asn Thr Ser Val Val 
            180                 185                 190         


Pro Glu Glu Pro Gln Met Glu Tyr Asn Ala Leu Arg Glu Tyr Lys Asp 
        195                 200                 205             


Ser Lys Ser Arg Asp Leu Leu Val Asp Tyr Ala Ala Leu Glu Leu Glu 
    210                 215                 220                 


Lys Lys Glu Arg Val Val Tyr Glu Asn Ser Ser Phe Leu Val Val Val 
225                 230                 235                 240 


Pro Tyr Trp Ala Val Trp Pro Phe Glu Thr Met Ile Phe Ser Lys Gln 
                245                 250                 255     


Arg Ile Ala Ser Val Ile Lys Leu Thr Lys Pro Gln Val Ala Asp Leu 
            260                 265                 270         


Ala Asp Ala Ile Ser Val Leu Thr Arg Lys Tyr Asp Lys Leu Phe Asn 
        275                 280                 285             


Thr Ser Phe Pro Tyr Ser Met Gly Ile His Gln Ser Phe Val Asp Glu 
    290                 295                 300                 


Pro Asn Cys Glu Tyr Glu His Leu Arg Leu Leu Phe Tyr Pro Pro Leu 
305                 310                 315                 320 


Leu Arg Ser Ala Ser Val Lys Lys Phe Gln Val Gly Phe Glu Met Leu 
                325                 330                 335     


Gly Met Pro Gln Arg Asp Ile Thr Ala Glu Gly Ala Ala Gln Arg Leu 
            340                 345                 350         


Lys Gly Leu 
        355 


<210>  19
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GAL10E consensus sequence


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Replace = Thr or nothing

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Replace = Tyr

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (34)..(34)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (44)..(44)
<223>  Replace = Ile or Val

<220>
<221>  VARIANT
<222>  (45)..(45)
<223>  Replace = Asp or Gln

<220>
<221>  VARIANT
<222>  (48)..(48)
<223>  Replace = Ser or Gln

<220>
<221>  VARIANT
<222>  (52)..(52)
<223>  Replace = His or Glu

<220>
<221>  VARIANT
<222>  (57)..(57)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (58)..(58)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (62)..(62)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (65)..(65)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (73)..(73)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (77)..(77)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (99)..(99)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (109)..(109)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (111)..(111)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (119)..(119)
<223>  Replace = His

<220>
<221>  VARIANT
<222>  (120)..(120)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (122)..(122)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (154)..(154)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (166)..(166)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (169)..(169)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (178)..(178)
<223>  Replace = Val or Pro

<220>
<221>  VARIANT
<222>  (179)..(179)
<223>  Replace = Ala or Ser

<220>
<221>  VARIANT
<222>  (184)..(184)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (213)..(213)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (230)..(230)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (235)..(235)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (258)..(258)
<223>  Replace = Asn, Asp or Lys

<220>
<221>  VARIANT
<222>  (262)..(262)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (264)..(264)
<223>  Replace = Glu or Gln

<220>
<221>  VARIANT
<222>  (265)..(265)
<223>  Replace = His

<220>
<221>  VARIANT
<222>  (266)..(266)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (268)..(268)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (269)..(269)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (272)..(272)
<223>  Replace = Glu or Lys

<220>
<221>  VARIANT
<222>  (273)..(273)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (275)..(275)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (291)..(291)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (296)..(296)
<223>  Replace = Met

<220>
<221>  VARIANT
<222>  (299)..(299)
<223>  Replace = Ile

<220>
<221>  VARIANT
<222>  (300)..(300)
<223>  Replace = Gln or Asp

<220>
<221>  VARIANT
<222>  (303)..(303)
<223>  Replace = Cys

<220>
<221>  VARIANT
<222>  (304)..(304)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (309)..(309)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (314)..(314)
<223>  Replace = Val

<220>
<221>  VARIANT
<222>  (315)..(315)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (330)..(330)
<223>  Replace = Asp

<220>
<221>  VARIANT
<222>  (334)..(334)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (337)..(337)
<223>  Replace = Glu, Ser or Gln

<220>
<221>  VARIANT
<222>  (339)..(339)
<223>  Replace = Arg

<220>
<221>  VARIANT
<222>  (341)..(341)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (342)..(342)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (344)..(344)
<223>  Replace = Met

<220>
<221>  VARIANT
<222>  (346)..(346)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (349)..(349)
<223>  Replace = Ala, Thr or Arg

<220>
<221>  VARIANT
<222>  (353)..(353)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (357)..(357)
<223>  Replace = Gln

<220>
<221>  VARIANT
<222>  (364)..(364)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (365)..(365)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (366)..(366)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (367)..(367)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (368)..(368)
<223>  Replace = nothing

<220>
<221>  VARIANT
<222>  (369)..(369)
<223>  Replace = Glu or nothing

<400>  19

Met Thr Gln Ala Ala Ala Glu His Val Leu Val Thr Gly Gly Ala Gly 
1               5                   10                  15      


Tyr Ile Gly Ser His Thr Val Ile Ala Leu Val Glu Ala Gly Arg Lys 
            20                  25                  30          


Pro Val Ile Val Asp Asn Leu Asp Asn Ser Cys Glu Glu Ala Val Arg 
        35                  40                  45              


Arg Ile Glu Gln Ile Val Gly Glu Lys Ile Pro Phe Phe Glu Val Asp 
    50                  55                  60                  


Ile Arg Asp Thr Asn Gly Leu Asp Glu Val Tyr Lys Gln Tyr Pro Thr 
65                  70                  75                  80  


Ile Thr Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser 
                85                  90                  95      


Gly Gln Ile Pro Leu Glu Tyr Tyr Asn Cys Asn Val Ala Gly Ser Ile 
            100                 105                 110         


Asn Leu Leu Gln Trp Cys Gln Lys His Asn Ile His Asn Phe Val Phe 
        115                 120                 125             


Ser Ser Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met 
    130                 135                 140                 


Ile Pro Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly 
145                 150                 155                 160 


Arg Thr Lys Met Met Ile Glu Asp Ile Ile Arg Asp His Val Arg Ala 
                165                 170                 175     


Asn Ala Pro Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly 
            180                 185                 190         


Ala His Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn 
        195                 200                 205             


Asn Leu Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys 
    210                 215                 220                 


Leu Phe Val Phe Gly Asp Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile 
225                 230                 235                 240 


Arg Asp Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala 
                245                 250                 255     


Leu Glu Lys Leu Glu Ala Val Asp Lys Glu Arg Lys Gln Leu Pro Gln 
            260                 265                 270         


Gly Ser Pro Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly 
        275                 280                 285             


Thr Gly His Gly Ser Thr Val Leu Glu Val Val Lys Ala Phe Ser Asn 
    290                 295                 300                 


Ala Val Gly Lys Asp Leu Pro Tyr Glu Ile Thr Gly Arg Arg Asp Gly 
305                 310                 315                 320 


Asp Val Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Ser Glu Leu 
                325                 330                 335     


Asn Trp Lys Ala Glu Lys Thr Leu Asp Glu Ser Cys Ser Asp Leu Trp 
            340                 345                 350         


Arg Trp Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Lys Asp Arg His Asp 
        355                 360                 365             


Lys 
    


<210>  20
<211>  369
<212>  PRT
<213>  Yarrowia galli

<400>  20

Met Thr Gln Ala Ala Ala Glu His Val Leu Val Thr Gly Gly Ala Gly 
1               5                   10                  15      


Tyr Ile Gly Ser His Thr Val Ile Ala Leu Val Glu Ala Gly Arg Lys 
            20                  25                  30          


Pro Val Ile Val Asp Asn Leu Asp Asn Ser Cys Glu Glu Ala Val Arg 
        35                  40                  45              


Arg Ile Glu Gln Ile Val Gly Glu Lys Ile Pro Phe Phe Glu Val Asp 
    50                  55                  60                  


Ile Arg Asp Thr Asn Gly Leu Asp Glu Val Tyr Lys Gln Tyr Pro Thr 
65                  70                  75                  80  


Ile Thr Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser 
                85                  90                  95      


Gly Gln Ile Pro Leu Glu Tyr Tyr Phe Cys Asn Val Ala Gly Ser Ile 
            100                 105                 110         


Asn Leu Leu Gln Trp Cys Gln Lys His Asp Ile His Asn Phe Val Phe 
        115                 120                 125             


Ser Ser Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met 
    130                 135                 140                 


Ile Pro Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly 
145                 150                 155                 160 


Arg Thr Lys Met Met Ile Glu Asp Ile Ile Arg Asp His Val Arg Ala 
                165                 170                 175     


Asn Ala Pro Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly 
            180                 185                 190         


Ala His Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn 
        195                 200                 205             


Asn Leu Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys 
    210                 215                 220                 


Leu Phe Val Phe Gly Asp Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile 
225                 230                 235                 240 


Arg Asp Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala 
                245                 250                 255     


Leu Asn Lys Leu Glu Ala Val Asp Lys Glu Arg Lys Gln Leu Pro Glu 
            260                 265                 270         


Asp Ser Ala Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly 
        275                 280                 285             


Thr Gly His Gly Ser Thr Val Leu Glu Val Val Lys Ala Phe Ser Lys 
    290                 295                 300                 


Ala Val Gly Lys Asp Leu Pro Tyr Glu Ile Ala Gly Arg Arg Asp Gly 
305                 310                 315                 320 


Asp Val Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Ser Glu Leu 
                325                 330                 335     


Glu Trp Lys Ala Glu Lys Thr Leu Asp Glu Ser Cys Ser Asp Leu Trp 
            340                 345                 350         


Arg Trp Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Lys Asp Arg His Asp 
        355                 360                 365             


Lys 
    


<210>  21
<211>  368
<212>  PRT
<213>  Yarrowia yakushimensis

<400>  21

Met Thr Gln Ala Thr Glu Tyr Val Leu Val Thr Gly Gly Ala Gly Tyr 
1               5                   10                  15      


Ile Gly Ser His Thr Val Ile Ala Leu Val Glu Ala Gly Arg Lys Pro 
            20                  25                  30          


Ile Ile Val Asp Asn Leu Asp Asn Ser Cys Glu Asp Ala Val Ser Arg 
        35                  40                  45              


Ile Glu His Ile Val Gly Glu Thr Ile Pro Phe Phe Lys Val Asp Ile 
    50                  55                  60                  


Arg Asp Thr Asn Gly Leu Asp Glu Val Tyr Lys Gln Phe Pro Thr Ile 
65                  70                  75                  80  


Thr Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser Gly 
                85                  90                  95      


Gln Ile Pro Leu Glu Tyr Tyr Asn Cys Asn Val Thr Gly Ser Ile Asn 
            100                 105                 110         


Leu Leu Gln Trp Cys Gln Lys His Asn Ile His Asn Phe Val Phe Ser 
        115                 120                 125             


Ser Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met Ile 
    130                 135                 140                 


Pro Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly Arg 
145                 150                 155                 160 


Thr Lys Met Met Val Glu Asp Ile Ile Arg Asp His Val Arg Ala Asn 
                165                 170                 175     


Val Pro Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly Ala 
            180                 185                 190         


His Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn Asn 
        195                 200                 205             


Leu Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys Leu 
    210                 215                 220                 


Phe Val Phe Gly Asn Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile Arg 
225                 230                 235                 240 


Asp Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala Leu 
                245                 250                 255     


Asp Lys Leu Glu Ala Val Asp Lys Glu Arg Lys Gln Leu Pro Glu Gly 
            260                 265                 270         


Ser Pro Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly Thr 
        275                 280                 285             


Gly His Gly Ser Thr Val Leu Glu Val Val Gln Ala Phe Ser Lys Ala 
    290                 295                 300                 


Val Gly Lys Asp Leu Pro Tyr Glu Ile Thr Gly Arg Arg Asp Gly Asp 
305                 310                 315                 320 


Val Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Thr Glu Leu Asn 
                325                 330                 335     


Trp Arg Ala Lys Gln Thr Leu Asp Lys Ser Cys Ala Asp Leu Trp Arg 
            340                 345                 350         


Trp Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Lys Asp Arg His Asp Glu 
        355                 360                 365             


<210>  22
<211>  365
<212>  PRT
<213>  Yarrowia phangngensis

<400>  22

Met Thr Gln Thr Glu His Val Leu Val Thr Gly Gly Ala Gly Tyr Ile 
1               5                   10                  15      


Gly Ser His Thr Val Ile Ser Leu Val Glu Ala Gly Arg Lys Pro Ile 
            20                  25                  30          


Ile Val Asp Asn Leu Asp Asn Ser Cys Ile Gln Ala Val Gln Arg Ile 
        35                  40                  45              


Glu Glu Ile Val Gly Glu Lys Ile Pro Phe Phe Glu Val Asp Val Arg 
    50                  55                  60                  


Asp Thr Lys Gly Leu Asp Glu Val Tyr Lys Gln Tyr Pro Thr Ile Thr 
65                  70                  75                  80  


Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser Gly Glu 
                85                  90                  95      


Ile Pro Leu Glu Tyr Tyr Phe Cys Asn Val Ala Gly Ser Ile Asn Leu 
            100                 105                 110         


Leu Gln Trp Cys His Lys His Asn Ile His Asn Phe Val Phe Ser Ser 
        115                 120                 125             


Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met Ile Pro 
    130                 135                 140                 


Ile Pro Glu Glu Cys Pro Thr Gly Pro Thr Asn Pro Tyr Gly Arg Thr 
145                 150                 155                 160 


Lys Met Met Ile Glu Asp Val Ile Arg Asp His Val Arg Ala Asn Pro 
                165                 170                 175     


Ala Trp Asn Ala Ala Ile Leu Arg Tyr Phe Asn Pro Met Gly Ala His 
            180                 185                 190         


Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn Asn Leu 
        195                 200                 205             


Leu Pro Tyr Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys Leu Phe 
    210                 215                 220                 


Val Phe Gly Asn Asp Tyr Pro Ser Arg Asp Gly Thr Pro Ile Arg Asp 
225                 230                 235                 240 


Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala Leu Asn 
                245                 250                 255     


Lys Leu Glu Ala Val Glu Lys Gln Arg Lys Glu Leu Pro Lys Asp Ser 
            260                 265                 270         


Ala Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly Thr Gly 
        275                 280                 285             


His Gly Ser Thr Val Leu Glu Val Val Asp Ala Phe Cys Lys Ala Val 
    290                 295                 300                 


Gly Lys Thr Leu Pro Tyr Glu Ile Thr Gly Arg Arg Asp Gly Asp Val 
305                 310                 315                 320 


Leu Asp Leu Thr Ala Lys Pro Gly Arg Ala Asn Ser Glu Leu Ser Trp 
                325                 330                 335     


Lys Ala Glu Lys Thr Leu Asp Glu Ser Cys Thr Asp Leu Trp Arg Trp 
            340                 345                 350         


Thr Ser Gln Asn Pro Tyr Gly Tyr Lys Lys Glu Glu Lys 
        355                 360                 365 


<210>  23
<211>  366
<212>  PRT
<213>  Yarrowia alimentaria

<400>  23

Met Thr Gln Thr Glu His Val Leu Val Thr Gly Gly Ala Gly Tyr Ile 
1               5                   10                  15      


Gly Ser His Thr Val Ile Ala Leu Ile Glu Ala Gly Arg Lys Pro Val 
            20                  25                  30          


Ile Val Asp Asn Leu Asp Asn Ser Cys Val Glu Ala Val Arg Arg Ile 
        35                  40                  45              


Glu Gln Ile Val Gly Glu Lys Val Pro Phe Phe Glu Val Asp Ile Arg 
    50                  55                  60                  


Asp Thr Asn Gly Leu Asp Ala Val Tyr Lys Glu Phe Pro Thr Ile Thr 
65                  70                  75                  80  


Ser Val Leu His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser Gly Gln 
                85                  90                  95      


Lys Pro Leu Glu Tyr Tyr Phe Cys Asn Val Ala Gly Thr Ile Asn Leu 
            100                 105                 110         


Leu Gln Trp Cys His Ser His Asn Ile His Asn Phe Val Phe Ser Ser 
        115                 120                 125             


Ser Ala Thr Val Tyr Gly Asp Val Thr Arg Phe Glu Gly Met Ile Pro 
    130                 135                 140                 


Ile Pro Glu Glu Cys Pro Thr Asp Pro Thr Asn Pro Tyr Gly Arg Thr 
145                 150                 155                 160 


Lys Met Met Ile Glu Asp Ile Ile Arg Asp His Val Arg Ala Asn Pro 
                165                 170                 175     


Ser Trp Asn Ala Ala Leu Leu Arg Tyr Phe Asn Pro Met Gly Ala His 
            180                 185                 190         


Pro Ser Gly Leu Val Gly Glu Asp Pro Leu Gly Ile Pro Asn Asn Leu 
        195                 200                 205             


Leu Pro Phe Leu Ala Gln Val Ala Val Gly Arg Arg Glu Lys Leu Phe 
    210                 215                 220                 


Val Phe Gly Asn Asp Tyr Pro Ser Lys Asp Gly Thr Pro Ile Arg Asp 
225                 230                 235                 240 


Tyr Ile His Val Cys Asp Leu Ala Ala Gly His Val Ala Ala Leu Lys 
                245                 250                 255     


Lys Leu Glu Ser Val Gln His Glu Arg Gln Glu Leu Pro Lys Asp Ser 
            260                 265                 270         


Pro Glu Leu Lys His Gly Phe Ser Arg Glu Trp Asn Leu Gly Thr Gly 
        275                 280                 285             


Leu Gly Ser Thr Val Met Glu Val Ile Lys Ala Phe Ser Lys Ala Val 
    290                 295                 300                 


Gly Lys Asp Leu Pro Tyr Glu Val Ala Gly Arg Arg Asp Gly Asp Val 
305                 310                 315                 320 


Leu Asp Leu Thr Ala Lys Pro Asp Arg Ala Asn Thr Glu Leu Gln Trp 
                325                 330                 335     


Lys Ala Glu Lys Thr Met Asp Glu Ser Cys Arg Asp Leu Trp Lys Trp 
            340                 345                 350         


Thr Ser Glu Asn Pro Tyr Gly Tyr Lys Glu Asp Arg His Asp 
        355                 360                 365     


<210>  24
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of GAL10M


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Replace = Phe

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Replace = Asp or Asn

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Replace = Glu, Asp or Gln

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  Replace = Val or Thr

<400>  24

Asn Pro Tyr Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr 
1               5                   10                  15      


Gln Pro Asn Gly Ile Leu Leu His Gly Gly 
            20                  25      


<210>  25
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of GAL10M


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Replace = Leu

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Replace = Pro

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Replace = Glu

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Replace = Thr or Lys

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  Replace = Asp or Val

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Replace = Lys

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Replace = Asp

<400>  25

Arg Leu Ser Arg Asp Ala Lys Leu Asp Thr Arg Asp Asp Pro Leu Val 
1               5                   10                  15      


Ser Val Ala Ile Leu Ser Arg Gly 
            20                  


<210>  26
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of GAL10M


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Replace = Asn

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Replace = Ser

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Replace = Ala or Ser

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Replace = Ala

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Replace = His or Tyr

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Replace = Gln or Glu

<220>
<221>  VARIANT
<222>  (28)..(28)
<223>  Replace = Lys or Arg

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  Replace = Asn, Asp or Lys

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Replace = Thr

<220>
<221>  VARIANT
<222>  (33)..(33)
<223>  Replace = Met

<400>  26

Glu Ser Arg Asp Phe Gly Arg Arg Ser Gly Val Ala Ile Glu Cys Ser 
1               5                   10                  15      


Arg Pro Thr Asn Ala Trp Asn Val Pro Gly Trp Gln Pro Trp Val Asp 
            20                  25                  30          


Leu 
    


<210>  27
<211>  329
<212>  PRT
<213>  Yarrowia galli

<400>  27

Met Thr Leu Cys Asn Thr Gly Ala Thr Leu Gln Ser Trp Glu Asn Ser 
1               5                   10                  15      


Val Leu Gly Phe Ser Asn Ala Asp Asn Tyr Val Ser Ser Glu Asn Pro 
            20                  25                  30          


Phe Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr Gln Pro 
        35                  40                  45              


Glu Gly Ile Val Leu His Gly Gly Lys Glu Ser Trp Ala Trp Lys Lys 
    50                  55                  60                  


Trp Thr Arg Thr Glu Glu Glu Gly His Val Thr Lys Phe Glu Leu Arg 
65                  70                  75                  80  


Asp Thr Glu Ile Thr Ala His Val Thr Tyr Thr Leu Thr Lys Pro Asp 
                85                  90                  95      


Ser Thr Thr Thr Gln Leu Glu Ile Glu Tyr Ser Ala Lys Ser Met Asp 
            100                 105                 110         


Gly Arg Ser Thr Pro Ile Ser Met Thr Asn His Ser Tyr Phe Cys Leu 
        115                 120                 125             


Asp Gln Asp Ser Glu Gly His Val Thr Val Asp Asp His Glu Leu Trp 
    130                 135                 140                 


Leu Cys Asn Asp His Val Ile Glu His Lys Asp Gly Leu Gln Ser Glu 
145                 150                 155                 160 


Ala Pro Thr Gly Glu Leu Val Cys Cys Glu Ala Leu Glu Arg Ala Glu 
                165                 170                 175     


Leu Val Lys Ser Arg Asp Gln Ile Glu Ser Arg Glu Ile Lys Gln Ser 
            180                 185                 190         


Arg Asp Ile Ser Asn Asp Ser Thr Ile Pro Leu Pro Gly Ser Ser Arg 
        195                 200                 205             


Ala Phe Ser Leu Ala Gly Leu Ser Leu Asp His Thr Phe Ala Val Asn 
    210                 215                 220                 


Asn Gln Arg Leu Ser Arg Asp Pro Lys Leu Asp Thr Arg Asp Asp Pro 
225                 230                 235                 240 


Leu Val Thr Val Ala Asp Leu Ser Arg Asp Lys Thr Arg Leu Ile Val 
                245                 250                 255     


Lys Thr Thr Glu Pro Cys Phe Gln Val Tyr Thr Gly Asp Tyr Asn Asn 
            260                 265                 270         


Val Ser Gln Leu Pro Gly Glu Ser Arg Asp Phe Gly Ala Arg Ser Gly 
        275                 280                 285             


Val Ala Ile Glu Cys Ser Arg Pro Thr Asn Ala Trp Asn Val Pro Gln 
    290                 295                 300                 


Trp Lys Asn Trp Val Asp Leu Gly Gln Gly Glu Tyr Gly Ala Lys Ile 
305                 310                 315                 320 


Val Tyr Val Leu Glu Lys Arg Gly Asp 
                325                 


<210>  28
<211>  319
<212>  PRT
<213>  Yarrowia yakushimensis

<400>  28

Met Thr Phe Ser Ala Thr Gly Ala Thr Leu Gln Ser Trp Glu Asn Cys 
1               5                   10                  15      


Ile Leu Gly Phe Glu Asn Asp Ala Asn Tyr Leu Asn Asn Gln Asn Pro 
            20                  25                  30          


Tyr Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr Asp Pro 
        35                  40                  45              


Asp Gly Ile Val Leu His Gly Gly Glu Glu Gly Trp Ser Trp Lys Asn 
    50                  55                  60                  


Trp Lys Lys Ile Glu Glu Thr Glu Lys Val Thr Lys Phe Glu Leu Asn 
65                  70                  75                  80  


Asp Asp Arg Ile Thr Ala His Val Ile Tyr Thr Leu Thr Asn Pro His 
                85                  90                  95      


Pro Asn Thr Ser Gln Leu Glu Ile Glu Tyr Ser Ala Leu Ala Lys Glu 
            100                 105                 110         


Lys Asp Ala Asn Leu Pro Ile Asn Met Thr Asn His Ser Tyr Phe Cys 
        115                 120                 125             


Leu Pro Pro Gln Ser Gly His Val Thr Val Asp Asp His Asp Leu Trp 
    130                 135                 140                 


Leu Cys Asn Thr Gln Val Leu Glu His Lys Asp Gly Leu Gln Ser Glu 
145                 150                 155                 160 


Pro Pro Thr Gly Lys Leu Ile Val Cys Asp Pro Leu Lys Glu Val Arg 
                165                 170                 175     


Ser Arg Asp Glu Asn Asn His Asp Asp Val Gly Ser His Asp Ser Asn 
            180                 185                 190         


Ser Pro Ser His Ala Val Gln Met Ser Leu Asn Gly Met Ala Leu Asp 
        195                 200                 205             


His Thr Phe Ala Val Asp Asn Glu Arg Leu Ser Arg Asp Ala Lys Leu 
    210                 215                 220                 


Asp Thr Arg Asp Asp Pro Leu Val Thr Val Ala Val Leu Ser Arg Asp 
225                 230                 235                 240 


Ser Lys Thr Arg Leu Thr Val Lys Thr Thr Glu Pro Cys Phe Gln Val 
                245                 250                 255     


Tyr Thr Gly Asp Phe Ile Ser Val Asp Gln Leu Ser Gly Glu Ser Arg 
            260                 265                 270         


Asp Phe Gly Ser Arg Ala Gly Val Ala Ile Glu Cys Ser Arg Pro Thr 
        275                 280                 285             


Asn Ala Trp His Val Pro Glu Trp Arg Asp Trp Val Asp Leu Ser Lys 
    290                 295                 300                 


Gly Val Tyr Gly Ser Lys Ile Val Tyr Ile Leu Glu Arg Glu Glu 
305                 310                 315                 


<210>  29
<211>  282
<212>  PRT
<213>  Yarrowia alimentaria

<400>  29

Met Tyr Ser Asp Ile Gly Ala Ala Val Lys Ala Leu Asp Gly Val Val 
1               5                   10                  15      


Leu Gly Phe Ser Asn Asp Glu Asn Tyr Leu Lys Pro Glu Asn Pro Phe 
            20                  25                  30          


Phe Gly Ala Thr Ile Gly Pro Val Ala Asn Arg Leu Tyr Asn Pro Gln 
        35                  40                  45              


Gly Ile Thr Leu His Gly Gly Ala Asn Ser Trp Ala Phe Lys Thr Trp 
    50                  55                  60                  


Ser Arg Ser Glu Asn Gly Asn Val Thr Thr Phe Asp Leu Val Asp Gly 
65                  70                  75                  80  


Asp Val Arg Ala Gln Val Lys Tyr Thr Leu Val Lys Thr Lys Thr Ala 
                85                  90                  95      


Thr Thr Leu Glu Ile Glu Phe Trp Ala Asn Ser Ser Gly Lys Ser Ile 
            100                 105                 110         


Pro Leu Ser Met Ala Asn His Cys Tyr Phe Ser Leu Asn Gly Glu Ser 
        115                 120                 125             


Thr Ile Asp Asp Ser Asp Leu Trp Leu Cys Ser Asp Lys Val Ile Glu 
    130                 135                 140                 


His Gly Asn Asp Ser Gln Asn Glu Ala Pro Thr Gly Gln Leu Lys Thr 
145                 150                 155                 160 


Phe Lys Arg Ala Ser Asn Ala Leu Thr Thr Pro Ile Asp His Thr Phe 
                165                 170                 175     


Val Ile Asp Glu Gln Arg Leu Leu Asn Asp Ala Lys Leu Asp Thr Arg 
            180                 185                 190         


Glu Asp Pro Leu Val Lys Val Ala Val Leu Ser Lys Gly Asn Val Ser 
        195                 200                 205             


Val Thr Val Glu Thr Thr Glu Pro Val Phe Gln Val Tyr Thr Gly Asp 
    210                 215                 220                 


Tyr Asn Asn Val Pro Arg Phe Glu Asn Glu Asn Arg Ser Phe Gly Ala 
225                 230                 235                 240 


Arg Ser Gly Val Ala Ile Glu Ala Ala Arg Pro Thr Asn Ala Trp Tyr 
                245                 250                 255     


Val Pro Gln Trp Lys Lys Trp Thr Asp Met Ala Gln Gly Asp Tyr Gly 
            260                 265                 270         


Ser Arg Thr Val Tyr Thr Val Lys Arg Gly 
        275                 280         


<210>  30
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
gcgcggatcc atgactaaat ctcattcaga agaagtgatt g                           41


<210>  31
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
gcgccctagg ttataattca tatagacagc tgcccaatgc                             40


<210>  32
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
gcgcggatcc atgactgctg aagaatttg                                         29


<210>  33
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
gcgccctagg ttacagtctt tgtagataat gaatc                                  35


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
gcgcggatcc atgacagctc agttacaaag                                        30


<210>  35
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
gcgccctagg tcaggaaaat ctgtagacaa tc                                     32


<210>  36
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
gtgaggatcc atgtctatca gcaccctgcc                                        30


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
gcgccctagg ttacaaatca acaatagcac atccc                                  35


<210>  38
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
gagaggatcc atgactcttg tggcatccg                                         29


<210>  39
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
gcgccctagg ttacaaatca tacaaccgct tgg                                    33


<210>  40
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
gagaagatct atgacccagg ctgctgctga ac                                     32


<210>  41
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
gcgcactagt ttacttgtca tgacggtcct ttttgtaacc                             40


<210>  42
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
gagaggatcc atgacctttt gcaacacggg agcaac                                 36


<210>  43
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
gcgccctagg tcactcccgc tccaaaacat agataatctt agc                         43


<210>  44
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
gcgcactagt atgtctatca gcaccctgcc                                        30


<210>  45
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
gcgccccggg ttacaaatca acaatagcac atccctc                                37


<210>  46
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
gagaactagt atgactcttg tggcatccg                                         29


<210>  47
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
gcgacccggg ttacaaatca tacaaccgct tggc                                   34


<210>  48
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
gagaactagt atgacccagg ctgctgctg                                         29


<210>  49
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
gagacccggg ttacttgtca tgacggtcct ttttg                                  35


<210>  50
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
gcgcactagt atgacctttt gcaacacggg agcaac                                 36


<210>  51
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  51
gcgcgaattc tcactcccgc tccaaaacat agataatctt ag                          42


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  52
tcttcaactg ctcctgtcct                                                   20


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  53
ctatcgtaat cgacaccatc c                                                 21


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  54
ccatcaaaaa gctgaccaag                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  55
atcgcagaag aggagggtag                                                   20


<210>  56
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  56
tggtgacgac tacccctct                                                    19


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  57
cggttcctag gttccattct                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  58
tccatctccc tcaatggaac                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  59
gccggtatag acctggaaac                                                   20


