
                                SEQUENCE LISTING

<110> Johnson, Matthew E.
      Xia, Qianghua
      Johnston, Brian T.
      Chesi, Alessandra
      Grant, Struan F.A.

<120> New Targets Modulated by a Casual
  Variant of Type 2 Diabetes (T2D) Embedded Within the TCF7L2
  Gene and Methods of Use Thereof for Identifying Agents
  Having Efficacy for the Treatment of Type 2 Diabetes and
  Other Metabolic Disorders

<130> 3460-P06189WO00

<140> PCT/US2016/037242   
<141> 2016-06-13  

<150> 62/174,009          
<151> 2015-06-11  

<160> 84

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 1
gactggagtt cagacgtgtg ctcttccgat ctccttcagg cttcaccagc ac         52

<210> 2
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 2
acactctttc cctacacgac gctcttccga tcttccactg agggaagtga aag        53

<210> 3
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 3
acactctttc cctacacgac gctcttccga tcttcctttt taaatggtga caaattc    57

<210> 4
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 4
gactggagtt cagacgtgtg ctcttccgat ctcttctctt tggtccggca ga         52

<210> 5
<211> 140
<212> DNA
<213> Artificial Sequence

<220> 
<223> Probe

<400> 5
gatctctgcc ggaccaaaga gaagattcct ttttaaatgg tgacaaattc atgggctttc 60
tctgcctcaa aacctagcac agctgttatt tactgaacaa ttagagagct aagcactttt 120
tagatactat ataatttaat                                             140

<210> 6
<211> 140
<212> DNA
<213> Artificial Sequence

<220> 
<223> Probe

<400> 6
ctcttcgcta taaacatttt agctttttgt gtttgctgac tggcaacaat acatagtgaa 60
agttctaata atttgtaatg cttttgcatg tctttgtatt tttcttggtt atcacatcac 120
atcaaattaa gatactgatc                                             140

<210> 7
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 7
acactctttc cctacacgac gctcttccga tcttccttca acctccactg agg        53

<210> 8
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 8
gactggagtt cagacgtgtg ctcttccgat ctcattggcc ttcaggcttc ac         52

<210> 9
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 9
caccgaacag ctgtgctagg ttttg                                       25

<210> 10
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 10
aaaccaaaac ctagcacagc tgttc                                       25

<210> 11
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 11
caccgcagta aataacagct gtgct                                       25

<210> 12
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 12
aaacagcaca gctgttattt actgc                                       25

<210> 13
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 13
caccgaaaac taagggtgcc tcata                                       25

<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 14
aaactatgag gcacccttag ttttc                                       25

<210> 15
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 15
caccgtttct cgtctgaaaa ctaa                                        24

<210> 16
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 16
aaacttagtt ttcagacgag aaac                                        24

<210> 17
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 17
caccggtttc tcgtctgaaa acta                                        24

<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 18
aaactagttt tcagacgaga aacc                                        24

<210> 19
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 19
caccgacttg ccttccctgt aactg                                       25

<210> 20
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 20
aaaccagtta cagggaaggc aagtc                                       25

<210> 21
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 21
caccgtataa tttaattgcc gtatg                                       25

<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 22
aaaccatacg gcaattaaat tatac                                       25

<210> 23
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 23
caccgagacg agaaaccaca gttac                                       25

<210> 24
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 24
aaacgtaact gtggtttctc gtctc                                       25

<210> 25
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 25
caccgacgag aaaccacagt taca                                        24

<210> 26
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 26
aaactgtaac tgtggtttct cgtc                                        24

<210> 27
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 27
caccgagaaa ccacagttac aggga                                       25

<210> 28
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 28
aaactccctg taactgtggt ttctc                                       25

<210> 29
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 29
caccgacaga tacgtcaggt gtcac                                       25

<210> 30
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 30
aaacgtgaca cctgacgtat ctgtc                                       25

<210> 31
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 31
caccggatta caatgcaagg ttgc                                        24

<210> 32
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 32
aaacgcaacc ttgcattgta atcc                                        24

<210> 33
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 33
caccgttaat catcttttgc acacg                                       25

<210> 34
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 34
aaaccgtgtg caaaagatga ttaac                                       25

<210> 35
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 35
caccgaagaa atttacagat acgtc                                       25

<210> 36
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 36
aaacgacgta tctgtaaatt tcttc                                       25

<210> 37
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 37
caccgtgtca ctggattaca atgca                                       25

<210> 38
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 38
aaactgcatt gtaatccagt gacac                                       25

<210> 39
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 39
caccgagtcg tctattacac aggcc                                       25

<210> 40
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 40
aaacggcctg tgtaatagac gactc                                       25

<210> 41
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 41
caccgtgtaa tagacgactt caca                                        24

<210> 42
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 42
aaactgtgaa gtcgtctatt acac                                        24

<210> 43
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 43
caccgtgtga agtcgtctat tacac                                       25

<210> 44
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 44
aaacgtgtaa tagacgactt cacac                                       25

<210> 45
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 45
caccgtggct aagtttccat gacc                                        24

<210> 46
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 46
aaacggtcat ggaaacttag ccac                                        24

<210> 47
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 47
caccgtccat gagatacttt gctgc                                       25

<210> 48
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 48
aaacgcagca aagtatctca tggac                                       25

<210> 49
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 49
cccggtaccc ctggtctcat cagcgatatt c                                31

<210> 50
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 50
cccctcgagg gcaccaagct aagacaagat a                                31

<210> 51
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 51
tctctgccgg accaaagaga agattcc                                     27

<210> 52
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 52
tgtgtccagg gcccctctaa ccttttc                                     27

<210> 53
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 53
tccattttca gtccggcag                                              19

<210> 54
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 54
catccttgag ggcttgtcta c                                           21

<210> 55
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 55
aggcatgata gtttctggga c                                           21

<210> 56
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 56
acctgacatc ccattgctg                                              19

<210> 57
<211> 240
<212> DNA
<213> Artificial Sequence

<220> 
<223> Upstream Sequence

<400> 57
cgtcctcttt ggctgaaggt ttttgcctgc aaccttgcat tgtaatccag tgacacctga 60
cgtatctgta aatttcttca aatttctaag tgtattacaa ccccgtgtgc aaaagatgat 120
taattaattg ccttgacagt aaaacaaaaa acaaaaaaaa ggtgtggggg tatatggtat 180
ccctgattta ctatagaaga tgcagagagt gaagggagat gaggtgggga ggaggggccc 240


<210> 58
<211> 240
<212> DNA
<213> Artificial Sequence

<220> 
<223> Downstream Sequence

<400> 58
cttgggttca aatgatcctc ctgcctcagc ctctcttaag cagtgccttt ccccattctc 60
atgggacttt ccaatccatg agatactttg ctgcagggaa gccctgtctg tccaggcctg 120
tgtaatagac gacttcacat ggtcctgtgt tgttgtttgc cttctgtgtg gctaagtttc 180
catgacctgg tggcttggaa gccccatccc tgatttgtgg gagaggcagg gaggcacctt 240


<210> 59
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 59
acagatacgt caggtgtcac tgg                                         23

<210> 60
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 60
ggattacaat gcaaggttgc agg                                         23

<210> 61
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 61
ttaatcatct tttgcacacg ggg                                         23

<210> 62
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 62
aagaaattta cagatacgtc agg                                         23

<210> 63
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 63
tgtcactgga ttacaatgca agg                                         23

<210> 64
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 64
agtcgtctat tacacaggcc tgg                                         23

<210> 65
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 65
gtgtaataga cgacttcaca tgg                                         23

<210> 66
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 66
tgtgaagtcg tctattacac agg                                         23

<210> 67
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 67
gtggctaagt ttccatgacc tgg                                         23

<210> 68
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 68
tccatgagat actttgctgc agg                                         23

<210> 69
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 69
ctctttggct gaaggttttt gcctgcaacc ttgcacacat ggtcctgtgt tgttgtttgc 60
cttctgt                                                           67

<210> 70
<211> 211
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 70
ccctggtctc atcagcgata ttcgtcctgc cttccttctg agtaatttcc atcttaggac 60
tggagtcagg tggagcaaga ttccatcgtt ggtttctgtt gggcctagag tgtcacactg 120
agacctaatt tcatacttta tgaattctag tactgctctc gaaggtaaga gccgtcctct 180
ttggctgaag gtttttgcct gcaaccttgc a                                211

<210> 71
<211> 211
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 71
ccctggtctc atcagcgata ttcgtcctgc cttccttctg agtaatttcc atcttaggac 60
tggagtcagg tggagcaaga ttccatcgtt ggtttctgtt gggcctagag tgtcacactg 120
agacctaatt tcatacttta tgaattctag tactgctctc gaaggtaaga gccgtcctct 180
ttggctgaag gtttttgcct gcaaccttgc a                                211

<210> 72
<211> 216
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 72
cacatggtcc tgtgttgttg tttgccttct gtgtggctaa gtttccatga cctggtggct 60
tggaagcccc atccctgatt tgtgggagag gcagggaggc accttgtagc gcactaggcg 120
ttgggcctga acaagtctgt gtgcttccaa tgtctttgtg gggaggttta cgagtccttc 180
ttattatata atagtatctt gtcttagctt ggtgcc                           216

<210> 73
<211> 216
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 73
cacatggtcc tgtgttgttg tttgccttct gtgtggctaa gtttccatga cctggtggct 60
tggaagcccc atccctgatt tgtgggagag gcagggaggc accttgtagc gcactaggcg 120
ttgggcctga acaagtctgt gtgcttccaa tgtctttgtg gggaggttta cgagtccttc 180
ttattatata atagtatctt gtcttagctt ggtgcc                           216

<210> 74
<211> 501
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 74
tccaattttt tcacatgtga agacatacac aaaagtttta ttggagggtt gcacatgtga 60
aagaaaaagg gagaaagcag gattgagcag ggggagccgt cagatggtaa tgcagatgtg 120
atgagatctc tgccggacca aagagaagat tcctttttaa atggtgacaa attcatgggc 180
tttctctgcc tcaaaaccta gcacagctgt tatttactga acaattagag agctaagcac 240
tttttagata ctatataatt taattgccgt atgaggcacc cttagttttc agacgagaaa 300
ccacagttac agggaaggca agtaacttag tcaatgtcag ataactagga aaaggttaga 360
ggggccctgg acacaggcct gtgtgactga gaagcttggg cacttcactg ctacatttca 420
tctcttcgct ataaacattt tagctttttg tgtttgctga ctggcaacaa tacatagtga 480
aagttctaat aatttgtaat g                                           501

<210> 75
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 75
aacagctgtg ctaggttttg agg                                         23

<210> 76
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 76
cagtaaataa cagctgtgct agg                                         23

<210> 77
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 77
aaaactaagg gtgcctcata cgg                                         23

<210> 78
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 78
gtttctcgtc tgaaaactaa ggg                                         23

<210> 79
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 79
ggtttctcgt ctgaaaacta agg                                         23

<210> 80
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 80
acttgccttc cctgtaactg tgg                                         23

<210> 81
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 81
tataatttaa ttgccgtatg agg                                         23

<210> 82
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 82
agacgagaaa ccacagttac agg                                         23

<210> 83
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 83
gacgagaaac cacagttaca ggg                                         23

<210> 84
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Guide Sequence

<400> 84
agaaaccaca gttacaggga agg                                         23
