                         SEQUENCE LISTING

<110>  New York University
       Arora, Paramjit S.
       Wuo, Michael G.
 
<120>  STABILIZED MINIMAL COILED-COIL MIMETICS

<130>  29527.1772

<140>  PCT/US16/36531
<141>  2016-06-08

<150>  62/172,669
<151>  2015-06-08

<150>  62/211,603
<151>  2015-08-28

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Unconstrained peptide A


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Glu at position 1 is acetylated

<400>  1

Glu Leu Ala Glu Leu Glu Trp Arg Leu 
1               5                   


<210>  2
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Unconstrained peptide B


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Leu at position 1 is acetylated

<400>  2

Leu Trp Glu Arg Ile Ala Arg Leu Arg 
1               5                   


<210>  3
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Hydrogen bond surrogate peptide AB-1


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is pentenoic acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is N-allylalanine

<400>  3

Xaa Glu Leu Xaa Glu Leu Glu Trp Arg Leu Gly Gly Ser Ser Gly Gly 
1               5                   10                  15      


Leu Ala Glu Arg Ile Ala Arg Leu Arg 
            20                  25  


<210>  4
<211>  30
<212>  PRT
<213>  Artificial

<220>
<223>  Cyclic peptide AB-2


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Position 1 and 30 are linked by a peptide bond to form a cyclic 
       peptide

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Position 1 and 30 are linked by a peptide bond to form a cyclic 
       peptide

<400>  4

Gly Gly Ser Ser Gly Gly Glu Leu Ala Glu Leu Glu Trp Arg Leu Gly 
1               5                   10                  15      


Gly Ser Asn Gly Gly Leu Ala Glu Arg Ile Ala Arg Leu Arg 
            20                  25                  30  


<210>  5
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  Disulfide-bridged peptide AB-3


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Position 2 and 23 are linked by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Position 2 and 23 are linked by a disulfide bridge

<400>  5

Glu Cys Ala Glu Leu Glu Trp Arg Leu Gly Gly Ser Ser Gly Gly Leu 
1               5                   10                  15      


Ala Glu Arg Ile Ala Arg Cys Arg 
            20                  


<210>  6
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-1


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:7 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<400>  6

Glu Trp Lys His Leu Xaa His Leu Leu Asn 
1               5                   10  


<210>  7
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-1


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:6 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  7

Lys Thr Arg Arg Ser Leu Thr Val Leu Xaa 
1               5                   10  


<210>  8
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-2 and CHD-NHR2-7


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:9 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<400>  8

Glu Leu Trp His Leu Xaa Glu Leu Leu Arg 
1               5                   10  


<210>  9
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-2 and CHD-NHR2-6


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:8 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  9

Glu Leu Trp Arg Ser Ile Arg Val Leu Xaa 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-3


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:11 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Cys at position 9 is linked to SEQ ID NO:11 by a disulfide bridge

<400>  10

Glu Leu Trp His Leu Xaa Glu Leu Cys Arg 
1               5                   10  


<210>  11
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-3


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cys at position 2 is linked to SEQ ID NO:10 by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:10 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  11

Glu Cys Trp Arg Ser Ile Arg Val Leu Xaa 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-4


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Cys at position 5 is linked to SEQ ID NO:13 by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:13 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<400>  12

Glu Leu Trp His Cys Xaa Glu Leu Leu Arg 
1               5                   10  


<210>  13
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-4


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Cys at position 6 is linked to SEQ ID NO:12 by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:12 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  13

Glu Leu Trp Arg Ser Cys Arg Val Leu Xaa 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-5


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cys at position 2 is linked to SEQ ID NO:15 by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:15 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<400>  14

Glu Cys Trp His Leu Xaa Glu Leu Leu Arg 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-5


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Cys at position 9 is linked to SEQ ID NO:14 by a disulfide bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:14 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  15

Glu Leu Trp Arg Ser Ile Arg Val Cys Xaa 
1               5                   10  


<210>  16
<211>  29
<212>  PRT
<213>  Artificial

<220>
<223>  ER transport peptide

<400>  16

Met Met Ser Phe Val Ser Leu Leu Leu Val Gly Ile Leu Phe Tyr Ala 
1               5                   10                  15      


Thr Glu Ala Glu Gln Leu Thr Lys Cys Glu Val Phe Gln 
            20                  25                  


<210>  17
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  ER retention signal

<400>  17

Lys Glu Asp Leu 
1               


<210>  18
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Nuclear transport peptide

<400>  18

Pro Pro Lys Lys Lys Arg Lys Val 
1               5               


<210>  19
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Mitochondrial transport peptide

<400>  19

Met Leu Ser Leu Arg Gln Ser Ile Arg Phe Phe Lys Pro Ala Thr Arg 
1               5                   10                  15      


Thr Leu Cys Ser Ser Arg Tyr Leu Leu 
            20                  25  


<210>  20
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide linker

<400>  20

Gly Gly Ser Ser Gly Gly 
1               5       


<210>  21
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide linker

<400>  21

Gly Gly Ser Asn Gly Gly 
1               5       


<210>  22
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Linear strand A from NHR2

<400>  22

Glu Trp Lys His Leu Asp His Leu Leu Asn 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Linear strand B from NHR2

<400>  23

Lys Thr Arg Arg Ser Leu Thr Val Leu Arg 
1               5                   10  


<210>  24
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand A of coiled-coil CHD-NHR2-6


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is linked to SEQ ID NO:9 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is azidolysine

<400>  24

Ala Leu Trp His Leu Xaa Glu Ala Leu Arg 
1               5                   10  


<210>  25
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Strand B of coiled-coil CHD-NHR2-7


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is linked to SEQ ID NO:8 by a triazole bridge

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is azidolysine

<400>  25

Glu Leu Trp Arg Ala Ile Arg Ala Leu Xaa 
1               5                   10  


