                         SEQUENCE LISTING

<110>  National Research Council of Canada
       O'Connor, Maureen
       Marcil, Anne
       Durocher, Yves
       Lenferink, Anne E. G.
 
<120>  Carbonic Anhydrase IX-Specific Antibodies and Uses Thereof

<130>  2015-050-02

<150>  US 62/173,405
<151>  2015-06-10

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR L1

<400>  1

Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ala 
1               5                   10      


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR L2

<400>  2

Asn Thr Ile Thr Leu Ala Asp 
1               5           


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR L3

<400>  3

Gln His Phe Trp Asn Ile Pro Phe Thr 
1               5                   


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR H1

<400>  4

Gly Phe Thr Phe Thr Ser Cys Tyr Ile His 
1               5                   10  


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR H2

<400>  5

Trp Ile Tyr Pro Gly Asn Gly Asn Thr Lys Tyr Asn Glu Ile Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C7 CDR H3

<400>  6

Gly Asp Thr Thr Ala Asn Thr Met Asp Tyr 
1               5                   10  


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR L1

<400>  7

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR L2

<400>  8

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR L3

<400>  9

Ser Gln Asn Thr His Val Pro Pro Thr 
1               5                   


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR H1

<400>  10

Gly Phe Thr Phe Asn Thr Tyr Ala Met Tyr 
1               5                   10  


<210>  11
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR H2

<400>  11

Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Asp 
            


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 CDR H3

<400>  12

Gly Trp Asp Trp Phe Ala Tyr 
1               5           


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR L1

<400>  13

Lys Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR L2

<400>  14

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR L3

<400>  15

Cys Gln Gly Thr His Phe Pro Trp 
1               5               


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR H1

<400>  16

Gly Tyr Thr Phe Thr Asn Tyr Gly Met Asn 
1               5                   10  


<210>  17
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR H2

<400>  17

Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 CDR H3

<400>  18

Gly Gly Ile Ala Thr Pro Thr Ser Tyr 
1               5                   


<210>  19
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 2C7 VL

<400>  19

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 
        35                  40                  45              


Tyr Asn Thr Ile Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Asn Ile Pro Phe 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  20
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 2C7 VH

<400>  20

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Cys 
            20                  25                  30          


Tyr Ile His Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Asn Gly Asn Thr Lys Tyr Asn Glu Ile Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Asp Thr Thr Ala Asn Thr Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Ser Val Thr Val Ser Ser 
        115                 


<210>  21
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 11H9 VL

<400>  21

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  22
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 11H9 VH

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Gly Arg Leu Val Gln Pro Lys Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln Ser Met 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Met Tyr 
                85                  90                  95      


Tyr Cys Val Arg Gly Trp Asp Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Pro Val Thr Val Ser Ala 
        115             


<210>  23
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 12H8 VL

<400>  23

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Cys Gln Gly 
                85                  90                  95      


Thr His Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  24
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb 12H8 VH

<400>  24

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Ile Ala Thr Pro Thr Ser Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser Ser 
        115             


<210>  25
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 2C7 Light chain

<400>  25

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 
        35                  40                  45              


Tyr Asn Thr Ile Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Asn Ile Pro Phe 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  26
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 2C7 Heavy Chain

<400>  26

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Cys 
            20                  25                  30          


Tyr Ile His Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Asn Gly Asn Thr Lys Tyr Asn Glu Ile Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Asp Thr Thr Ala Asn Thr Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


<210>  27
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 11H9 Light Chain

<400>  27

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  28
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 11H9 Heavy chain

<400>  28

Glu Val Gln Leu Val Glu Ser Gly Gly Arg Leu Val Gln Pro Lys Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln Ser Met 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Met Tyr 
                85                  90                  95      


Tyr Cys Val Arg Gly Trp Asp Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Pro Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445         


<210>  29
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 12H8 Light Chain

<400>  29

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Cys Gln Gly 
                85                  90                  95      


Thr His Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  30
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric 12H8 Heavy chain

<400>  30

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Ile Ala Thr Pro Thr Ser Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445         


<210>  31
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain signal sequence

<400>  31

Met Val Leu Gln Thr Gln Val Phe Ile Ser Leu Leu Leu Trp Ile Ser 
1               5                   10                  15      


Gly Ala Tyr Gly 
            20  


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain signal sequence

<400>  32

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala 
            


<210>  33
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant hCA-IX ECD

<400>  33

Met Ala Pro Leu Cys Pro Ser Pro Trp Leu Pro Leu Leu Ile Pro Ala 
1               5                   10                  15      


Pro Ala Pro Gly Leu Thr Val Gln Leu Leu Leu Ser Leu Leu Leu Leu 
            20                  25                  30          


Val Pro Val His Pro Gln Arg Leu Pro Arg Met Gln Glu Asp Ser Pro 
        35                  40                  45              


Leu Gly Gly Gly Ser Ser Gly Glu Asp Asp Pro Leu Gly Glu Glu Asp 
    50                  55                  60                  


Leu Pro Ser Glu Glu Asp Ser Pro Arg Glu Glu Asp Pro Pro Gly Glu 
65                  70                  75                  80  


Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro 
                85                  90                  95      


Glu Val Lys Pro Lys Ser Glu Glu Glu Gly Ser Leu Lys Leu Glu Asp 
            100                 105                 110         


Leu Pro Thr Val Glu Ala Pro Gly Asp Pro Gln Glu Pro Gln Asn Asn 
        115                 120                 125             


Ala His Arg Asp Lys Glu Gly Asp Asp Gln Ser His Trp Arg Tyr Gly 
    130                 135                 140                 


Gly Asp Pro Pro Trp Pro Arg Val Ser Pro Ala Cys Ala Gly Arg Phe 
145                 150                 155                 160 


Gln Ser Pro Val Asp Ile Arg Pro Gln Leu Ala Ala Phe Cys Pro Ala 
                165                 170                 175     


Leu Arg Pro Leu Glu Leu Leu Gly Phe Gln Leu Pro Pro Leu Pro Glu 
            180                 185                 190         


Leu Arg Leu Arg Asn Asn Gly His Ser Val Gln Leu Thr Leu Pro Pro 
        195                 200                 205             


Gly Leu Glu Met Ala Leu Gly Pro Gly Arg Glu Tyr Arg Ala Leu Gln 
    210                 215                 220                 


Leu His Leu His Trp Gly Ala Ala Gly Arg Pro Gly Ser Glu His Thr 
225                 230                 235                 240 


Val Glu Gly His Arg Phe Pro Ala Glu Ile His Val Val His Leu Ser 
                245                 250                 255     


Thr Ala Phe Ala Arg Val Asp Glu Ala Leu Gly Arg Pro Gly Gly Leu 
            260                 265                 270         


Ala Val Leu Ala Ala Phe Leu Glu Glu Gly Pro Glu Glu Asn Ser Ala 
        275                 280                 285             


Tyr Glu Gln Leu Leu Ser Arg Leu Glu Glu Ile Ala Glu Glu Gly Ser 
    290                 295                 300                 


Glu Thr Gln Val Pro Gly Leu Asp Ile Ser Ala Leu Leu Pro Ser Asp 
305                 310                 315                 320 


Phe Ser Arg Tyr Phe Gln Tyr Glu Gly Ser Leu Thr Thr Pro Pro Cys 
                325                 330                 335     


Ala Gln Gly Val Ile Trp Thr Val Phe Asn Gln Thr Val Met Leu Ser 
            340                 345                 350         


Ala Lys Gln Leu His Thr Leu Ser Asp Thr Leu Trp Gly Pro Gly Asp 
        355                 360                 365             


Ser Arg Leu Gln Leu Asn Phe Arg Ala Thr Gln Pro Leu Asn Gly Arg 
    370                 375                 380                 


Val Ile Glu Ala Ser Phe Pro Ala Gly Val Asp Ser Ser Pro Arg Ala 
385                 390                 395                 400 


Ala Glu Pro Val Gln Leu Asn Ser Cys Leu Ala Ala Gly Asp Gly Ser 
                405                 410                 415     


His His His His His His His His His His Gly 
            420                 425         


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 epitope

<400>  34

Glu Glu Asp Leu Pro Gly Glu Glu 
1               5               


<210>  35
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 epitope

<400>  35

Leu Pro Arg Met Gln Glu Asp Ser Pro Leu Gly Gly Gly 
1               5                   10              


<210>  36
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Putative 2C7 epitope

<400>  36

Pro Ser Asp Phe Ser Arg Tyr Phe Gln Tyr Glu Gly Ser Leu 
1               5                   10                  


<210>  37
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA-IX repeat sequence

<400>  37

Gly Glu Glu Asp Leu Pro 
1               5       


<210>  38
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 37-51

<400>  38

Pro Gln Arg Leu Pro Arg Met Gln Glu Asp Ser Pro Leu Gly Gly 
1               5                   10                  15  


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 47-61

<400>  39

Ser Pro Leu Gly Gly Gly Ser Ser Gly Glu Asp Asp Pro Leu Gly 
1               5                   10                  15  


<210>  40
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 57-71

<400>  40

Asp Asp Pro Leu Gly Glu Glu Asp Leu Pro Ser Glu Glu Asp Ser 
1               5                   10                  15  


<210>  41
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 67-81

<400>  41

Ser Glu Glu Asp Ser Pro Arg Glu Glu Asp Pro Pro Gly Glu Glu 
1               5                   10                  15  


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 77-91

<400>  42

Pro Pro Gly Glu Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro Gly 
1               5                   10                  15  


<210>  43
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 87-101

<400>  43

Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro Glu Val Lys Pro Lys 
1               5                   10                  15  


<210>  44
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 97-111

<400>  44

Glu Val Lys Pro Lys Ser Glu Glu Glu Gly Ser Leu Lys Leu Glu 
1               5                   10                  15  


<210>  45
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 107-121

<400>  45

Ser Leu Lys Leu Glu Asp Leu Pro Thr Val Glu Ala Pro Gly Asp 
1               5                   10                  15  


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 117-131

<400>  46

Glu Ala Pro Gly Asp Pro Gln Glu Pro Gln Asn Asn Ala His Arg 
1               5                   10                  15  


<210>  47
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 126-140

<400>  47

Gln Asn Asn Ala His Arg Asp Lys Glu Gly Asp Asp Gln Ser His 
1               5                   10                  15  


<210>  48
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 37-71

<400>  48

Pro Gln Arg Leu Pro Arg Met Gln Glu Asp Ser Pro Leu Gly Gly Gly 
1               5                   10                  15      


Ser Ser Gly Glu Asp Asp Pro Leu Gly Glu Glu Asp Leu Pro Ser Glu 
            20                  25                  30          


Glu Asp Ser 
        35  


<210>  49
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 67-111

<400>  49

Ser Glu Glu Asp Ser Pro Arg Glu Glu Asp Pro Pro Gly Glu Glu Asp 
1               5                   10                  15      


Leu Pro Gly Glu Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro Glu Val 
            20                  25                  30          


Lys Pro Lys Ser Glu Glu Glu Gly Ser Leu Lys Leu Glu 
        35                  40                  45  


<210>  50
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 107-140

<400>  50

Ser Leu Lys Leu Glu Asp Leu Pro Thr Val Glu Ala Pro Gly Asp Pro 
1               5                   10                  15      


Gln Glu Pro Gln Asn Asn Ala His Arg Asp Lys Glu Gly Asp Asp Gln 
            20                  25                  30          


Ser His 
        


<210>  51
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCA-IX amino acid 37-140

<400>  51

Pro Gln Arg Leu Pro Arg Met Gln Glu Asp Ser Pro Leu Gly Gly Gly 
1               5                   10                  15      


Ser Ser Gly Glu Asp Asp Pro Leu Gly Glu Glu Asp Leu Pro Ser Glu 
            20                  25                  30          


Glu Asp Ser Pro Arg Glu Glu Asp Pro Pro Gly Glu Glu Asp Leu Pro 
        35                  40                  45              


Gly Glu Glu Asp Leu Pro Gly Glu Glu Asp Leu Pro Glu Val Lys Pro 
    50                  55                  60                  


Lys Ser Glu Glu Glu Gly Ser Leu Lys Leu Glu Asp Leu Pro Thr Val 
65                  70                  75                  80  


Glu Ala Pro Gly Asp Pro Gln Glu Pro Gln Asn Asn Ala His Arg Asp 
                85                  90                  95      


Lys Glu Gly Asp Asp Gln Ser His 
            100                 


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  12H8 minimal epitope

<400>  52

Leu Pro Arg Met Gln Glu Asp Ser Pro 
1               5                   


<210>  53
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11H9 minimal epitope

<400>  53

Glu Asp Leu Pro Gly Glu Glu Asp 
1               5               


