               SEQUENCE LISTING

<110> Glycom A/S

<120> Mutated Sialidases

<130> DAS/P19659WO

<150> EP15171175.1
<151> 2015-06-09

<160> 3

<170> BiSSAP 1.3.6

<210> 1
<211> 497
<212> PRT
<213> Photobacterium leiognathi JT-SHIZ-119


<400> 1
Met Cys Asn Asp Asn Gln Asn Thr Val Asp Val Val Val Ser Thr Val 
1               5                   10                  15      
Asn Asp Asn Val Ile Glu Asn Asn Thr Tyr Gln Val Lys Pro Ile Asp 
            20                  25                  30          
Thr Pro Thr Thr Phe Asp Ser Tyr Ser Trp Ile Gln Thr Cys Gly Thr 
        35                  40                  45              
Pro Ile Leu Lys Asp Asp Glu Lys Tyr Ser Leu Ser Phe Asp Phe Val 
    50                  55                  60                  
Ala Pro Glu Leu Asp Gln Asp Glu Lys Phe Cys Phe Glu Phe Thr Gly 
65                  70                  75                  80  
Asp Val Asp Gly Lys Arg Tyr Val Thr Gln Thr Asn Leu Thr Val Val 
                85                  90                  95      
Ala Pro Thr Leu Glu Val Tyr Val Asp His Ala Ser Leu Pro Ser Leu 
            100                 105                 110         
Gln Gln Leu Met Lys Ile Ile Gln Gln Lys Asn Glu Tyr Ser Gln Asn 
        115                 120                 125             
Glu Arg Phe Ile Ser Trp Gly Arg Ile Gly Leu Thr Glu Asp Asn Ala 
    130                 135                 140                 
Glu Lys Leu Asn Ala His Ile Tyr Pro Leu Ala Gly Asn Asn Thr Ser 
145                 150                 155                 160 
Gln Glu Leu Val Asp Ala Val Ile Asp Tyr Ala Asp Ser Lys Asn Arg 
                165                 170                 175     
Leu Asn Leu Glu Leu Asn Thr Asn Thr Ala His Ser Phe Pro Asn Leu 
            180                 185                 190         
Ala Pro Ile Leu Arg Ile Ile Ser Ser Lys Ser Asn Ile Leu Ile Ser 
        195                 200                 205             
Asn Ile Asn Leu Tyr Asp Asp Gly Ser Ala Glu Tyr Val Asn Leu Tyr 
    210                 215                 220                 
Asn Trp Lys Asp Thr Glu Asp Lys Ser Val Lys Leu Ser Asp Ser Phe 
225                 230                 235                 240 
Leu Val Leu Lys Asp Tyr Phe Asn Gly Ile Ser Ser Glu Lys Pro Ser 
                245                 250                 255     
Gly Ile Tyr Gly Arg Tyr Asn Trp His Gln Leu Tyr Asn Thr Ser Tyr 
            260                 265                 270         
Tyr Phe Leu Arg Lys Asp Tyr Leu Thr Val Glu Pro Gln Leu His Asp 
        275                 280                 285             
Leu Arg Glu Tyr Leu Gly Gly Ser Leu Lys Gln Met Ser Trp Asp Gly 
    290                 295                 300                 
Phe Ser Gln Leu Ser Lys Gly Asp Lys Glu Leu Phe Leu Asn Ile Val 
305                 310                 315                 320 
Gly Phe Asp Gln Glu Lys Leu Gln Gln Glu Tyr Gln Gln Ser Glu Leu 
                325                 330                 335     
Pro Asn Phe Val Phe Thr Gly Thr Thr Thr Trp Ala Gly Gly Glu Thr 
            340                 345                 350         
Lys Glu Tyr Tyr Ala Gln Gln Gln Val Asn Val Val Asn Asn Ala Ile 
        355                 360                 365             
Asn Glu Thr Ser Pro Tyr Tyr Leu Gly Arg Glu His Asp Leu Phe Phe 
    370                 375                 380                 
Lys Gly His Pro Arg Gly Gly Ile Ile Asn Asp Ile Ile Leu Gly Ser 
385                 390                 395                 400 
Phe Asn Asn Met Ile Asp Ile Pro Ala Lys Val Ser Phe Glu Val Leu 
                405                 410                 415     
Met Met Thr Gly Met Leu Pro Asp Thr Val Gly Gly Ile Ala Ser Ser 
            420                 425                 430         
Leu Tyr Phe Ser Ile Pro Ala Glu Lys Val Ser Phe Ile Val Phe Thr 
        435                 440                 445             
Ser Ser Asp Thr Ile Thr Asp Arg Glu Asp Ala Leu Lys Ser Pro Leu 
    450                 455                 460                 
Val Gln Val Met Met Thr Leu Gly Ile Val Lys Glu Lys Asp Val Leu 
465                 470                 475                 480 
Phe Trp Ser Asp Leu Pro Asp Cys Ser Ser Gly Val Cys Ile Ala Gln 
                485                 490                 495     
Tyr 
    

<210> 2
<211> 483
<212> PRT
<213> Photobacterium leiognathi JT-SHIZ-145


<400> 2
Met Cys Asn Asp Asn Gln Asn Thr Val Asp Val Val Val Ser Thr Val 
1               5                   10                  15      
Asn Asp Asn Val Ile Glu Asn Asn Thr Tyr Gln Val Lys Pro Ile Asp 
            20                  25                  30          
Thr Pro Thr Thr Phe Asp Ser Tyr Ser Trp Ile Gln Thr Cys Gly Thr 
        35                  40                  45              
Pro Ile Leu Lys Asp Asp Glu Lys Tyr Ser Leu Ser Phe Asp Phe Val 
    50                  55                  60                  
Ala Pro Glu Leu Asp Gln Asp Glu Lys Phe Cys Phe Glu Phe Thr Gly 
65                  70                  75                  80  
Asp Val Asp Gly Lys Arg Tyr Val Thr Gln Thr Asn Leu Thr Val Val 
                85                  90                  95      
Ala Pro Thr Leu Glu Val Tyr Val Asp His Ala Ser Leu Pro Ser Leu 
            100                 105                 110         
Gln Gln Leu Met Lys Ile Ile Gln Gln Lys Asn Glu Tyr Ser Gln Asn 
        115                 120                 125             
Glu Arg Phe Ile Ser Trp Gly Arg Ile Arg Leu Thr Glu Asp Asn Ala 
    130                 135                 140                 
Glu Lys Leu Asn Ala His Ile Tyr Pro Leu Ala Gly Asn Asn Thr Ser 
145                 150                 155                 160 
Gln Glu Leu Val Asp Ala Val Ile Asp Tyr Ala Asp Ser Lys Asn Arg 
                165                 170                 175     
Leu Asn Leu Glu Leu Asn Thr Asn Thr Gly His Ser Phe Arg Asn Ile 
            180                 185                 190         
Ala Pro Ile Leu Arg Ala Thr Ser Ser Lys Asn Asn Ile Leu Ile Ser 
        195                 200                 205             
Asn Ile Asn Leu Tyr Asp Asp Gly Ser Ala Glu Tyr Val Ser Leu Tyr 
    210                 215                 220                 
Asn Trp Lys Asp Thr Asp Asn Lys Ser Gln Lys Leu Ser Asp Ser Phe 
225                 230                 235                 240 
Leu Val Leu Lys Asp Tyr Leu Asn Gly Ile Ser Ser Glu Lys Pro Asn 
                245                 250                 255     
Gly Ile Tyr Ser Ile Tyr Asn Trp His Gln Leu Tyr His Ser Ser Tyr 
            260                 265                 270         
Tyr Phe Leu Arg Lys Asp Tyr Leu Thr Val Glu Thr Lys Leu His Asp 
        275                 280                 285             
Leu Arg Glu Tyr Leu Gly Gly Ser Leu Lys Gln Met Ser Trp Asp Thr 
    290                 295                 300                 
Phe Ser Gln Leu Ser Lys Gly Asp Lys Glu Leu Phe Leu Asn Ile Val 
305                 310                 315                 320 
Gly Phe Asp Gln Glu Lys Leu Gln Gln Glu Tyr Gln Gln Ser Glu Leu 
                325                 330                 335     
Pro Asn Phe Val Phe Thr Gly Thr Thr Thr Trp Ala Gly Gly Glu Thr 
            340                 345                 350         
Lys Glu Tyr Tyr Ala Gln Gln Gln Val Asn Val Val Asn Asn Ala Ile 
        355                 360                 365             
Asn Glu Thr Ser Pro Tyr Tyr Leu Gly Arg Glu His Asp Leu Phe Phe 
    370                 375                 380                 
Lys Gly His Pro Arg Gly Gly Ile Ile Asn Asp Ile Ile Leu Gly Ser 
385                 390                 395                 400 
Phe Asn Asn Met Ile Asp Ile Pro Ala Lys Val Ser Phe Glu Val Leu 
                405                 410                 415     
Met Met Thr Gly Met Leu Pro Asp Thr Val Gly Gly Ile Ala Ser Ser 
            420                 425                 430         
Leu Tyr Phe Ser Ile Pro Ala Glu Lys Val Ser Phe Ile Val Phe Thr 
        435                 440                 445             
Ser Ser Asp Thr Ile Thr Asp Arg Glu Asp Ala Leu Lys Ser Pro Leu 
    450                 455                 460                 
Val Gln Val Met Met Thr Leu Gly Ile Val Lys Glu Lys Asp Val Leu 
465                 470                 475                 480 
Phe Trp Cys 
            

<210> 3
<211> 661
<212> PRT
<213> Photobacterium damselae JT0160


<400> 3
Met Cys Asn Ser Asp Asn Thr Ser Leu Lys Glu Thr Val Ser Ser Asn 
1               5                   10                  15      
Ser Ala Asp Val Val Glu Thr Glu Thr Tyr Gln Leu Thr Pro Ile Asp 
            20                  25                  30          
Ala Pro Ser Ser Phe Leu Ser His Ser Trp Glu Gln Thr Cys Gly Thr 
        35                  40                  45              
Pro Ile Leu Asn Glu Ser Asp Lys Gln Ala Ile Ser Phe Asp Phe Val 
    50                  55                  60                  
Ala Pro Glu Leu Lys Gln Asp Glu Lys Tyr Cys Phe Thr Phe Lys Gly 
65                  70                  75                  80  
Ile Thr Gly Asp His Arg Tyr Ile Thr Asn Thr Thr Leu Thr Val Val 
                85                  90                  95      
Ala Pro Thr Leu Glu Val Tyr Ile Asp His Ala Ser Leu Pro Ser Leu 
            100                 105                 110         
Gln Gln Leu Ile His Ile Ile Gln Ala Lys Asp Glu Tyr Pro Ser Asn 
        115                 120                 125             
Gln Arg Phe Val Ser Trp Lys Arg Val Thr Val Asp Ala Asp Asn Ala 
    130                 135                 140                 
Asn Lys Leu Asn Ile His Thr Tyr Pro Leu Lys Gly Asn Asn Thr Ser 
145                 150                 155                 160 
Pro Glu Met Val Ala Ala Ile Asp Glu Tyr Ala Gln Ser Lys Asn Arg 
                165                 170                 175     
Leu Asn Ile Glu Phe Tyr Thr Asn Thr Ala His Val Phe Asn Asn Leu 
            180                 185                 190         
Pro Pro Ile Ile Gln Pro Leu Tyr Asn Asn Glu Lys Val Lys Ile Ser 
        195                 200                 205             
His Ile Ser Leu Tyr Asp Asp Gly Ser Ser Glu Tyr Val Ser Leu Tyr 
    210                 215                 220                 
Gln Trp Lys Asp Thr Pro Asn Lys Ile Glu Thr Leu Glu Gly Glu Val 
225                 230                 235                 240 
Ser Leu Leu Ala Asn Tyr Leu Ala Gly Thr Ser Pro Asp Ala Pro Lys 
                245                 250                 255     
Gly Met Gly Asn Arg Tyr Asn Trp His Lys Leu Tyr Asp Thr Asp Tyr 
            260                 265                 270         
Tyr Phe Leu Arg Glu Asp Tyr Leu Asp Val Glu Ala Asn Leu His Asp 
        275                 280                 285             
Leu Arg Asp Tyr Leu Gly Ser Ser Ala Lys Gln Met Pro Trp Asp Glu 
    290                 295                 300                 
Phe Ala Lys Leu Ser Asp Ser Gln Gln Thr Leu Phe Leu Asp Ile Val 
305                 310                 315                 320 
Gly Phe Asp Lys Glu Gln Leu Gln Gln Gln Tyr Ser Gln Ser Pro Leu 
                325                 330                 335     
Pro Asn Phe Ile Phe Thr Gly Thr Thr Thr Trp Ala Gly Gly Glu Thr 
            340                 345                 350         
Lys Glu Tyr Tyr Ala Gln Gln Gln Val Asn Val Ile Asn Asn Ala Ile 
        355                 360                 365             
Asn Glu Thr Ser Pro Tyr Tyr Leu Gly Lys Asp Tyr Asp Leu Phe Phe 
    370                 375                 380                 
Lys Gly His Pro Ala Gly Gly Val Ile Asn Asp Ile Ile Leu Gly Ser 
385                 390                 395                 400 
Phe Pro Asp Met Ile Asn Ile Pro Ala Lys Ile Ser Phe Glu Val Leu 
                405                 410                 415     
Met Met Thr Asp Met Leu Pro Asp Thr Val Ala Gly Ile Ala Ser Ser 
            420                 425                 430         
Leu Tyr Phe Thr Ile Pro Ala Asp Lys Val Asn Phe Ile Val Phe Thr 
        435                 440                 445             
Ser Ser Asp Thr Ile Thr Asp Arg Glu Glu Ala Leu Lys Ser Pro Leu 
    450                 455                 460                 
Val Gln Val Met Leu Thr Leu Gly Ile Val Lys Glu Lys Asp Val Leu 
465                 470                 475                 480 
Phe Trp Ala Asp His Lys Val Asn Ser Met Glu Val Ala Ile Asp Glu 
                485                 490                 495     
Ala Cys Thr Arg Ile Ile Ala Lys Arg Gln Pro Thr Ala Ser Asp Leu 
            500                 505                 510         
Arg Leu Val Ile Ala Ile Ile Lys Thr Ile Thr Asp Leu Glu Arg Ile 
        515                 520                 525             
Gly Asp Val Ala Glu Ser Ile Ala Lys Val Ala Leu Glu Ser Phe Ser 
    530                 535                 540                 
Asn Lys Gln Tyr Asn Leu Leu Val Ser Leu Glu Ser Leu Gly Gln His 
545                 550                 555                 560 
Thr Val Arg Met Leu His Glu Val Leu Asp Ala Phe Ala Arg Met Asp 
                565                 570                 575     
Val Lys Ala Ala Ile Glu Val Tyr Gln Glu Asp Asp Arg Ile Asp Gln 
            580                 585                 590         
Glu Tyr Glu Ser Ile Val Arg Gln Leu Met Ala His Met Met Glu Asp 
        595                 600                 605             
Pro Ser Ser Ile Pro Asn Val Met Lys Val Met Trp Ala Ala Arg Ser 
    610                 615                 620                 
Ile Glu Arg Val Gly Asp Arg Cys Gln Asn Ile Cys Glu Tyr Ile Ile 
625                 630                 635                 640 
Tyr Phe Val Lys Gly Lys Asp Val Arg His Thr Lys Pro Asp Asp Phe 
                645                 650                 655     
Gly Thr Met Leu Asp 
            660     

