                         SEQUENCE LISTING

<110>  Ironwood Pharmaceuticals, Inc.
 
<120>  Modified or Targeted Release Formulations of Linaclotide

<130>  223355/158PCT1/395990

<140>  tbd
<141>  2016-06-03

<150>  62/171,462
<151>  2016-05-20

<150>  62/339,462
<151>  2015-06-05

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  1

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  2
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa1 is a Cys, wherein the alpha-amine of the Cys is deaminated.

<220>
<221>  DISULFID
<222>  (1)..(6)
<223>  Disulfide bond is between the deaminated alpha-amine of Xaa1 and 
       Cys6.

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  2

Xaa Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  3
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)
<223>  Trisulfide bond between Cys1 and Cys6.

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  3

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  4
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  MISC_FEATURE
<222>  (5)..(13)
<223>  Trisulfide bond between Cys5 and Cys13.

<400>  4

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  5
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated sequence


<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  MISC_FEATURE
<222>  (2)..(10)
<223>  Trisulfide bond between Cys2 and Cys10.

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  5

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa1 is a Cys, wherein the alpha-amine of the Cys which is 
       cross-linked to the amine group of Cys2 to form an 
       imidazolidinone 5 membered ring at the N-terminus of the peptide.

<220>
<221>  DISULFID
<222>  (1)..(6)
<223>  Disulfide bond is between the imidazolidinone 5-membered ring of 
       Xaa1 and Cys6.

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  6

Xaa Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  7
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (2)..(13)

<400>  7

Cys Cys Glu Tyr Cys Cys Asp Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  8
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  ACETYLATION

<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  8

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 
1               5                   10                  


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetically generated peptide


<220>
<221>  DISULFID
<222>  (1)..(6)

<220>
<221>  DISULFID
<222>  (2)..(10)

<220>
<221>  DISULFID
<222>  (5)..(13)

<400>  9

Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys 
1               5                   10              


