                         SEQUENCE LISTING
<110>  BASF SE

<120>  Recombinant microorganism for improved production of 
       fine chemicals

<130>  0000078588 
<150>  US 62/171036
<151>  2015 06 04
<160>  125

<170>  According WIPO Sd. 25

<210> 1
<211> 1320
<212> DNA
<213> Escherichia coli
<220>
<223> brnQ
<400> 1
atgacccatc aattaagatc gcgcgatatc atcgctctgg gctttatgac atttgcgttg     60
ttcgtcggcg caggtaacat tattttccct ccaatggtcg gcttgcaggc aggcgaacac    120
gtctggactg cggcattcgg cttcctcatt actgccgttg gcctgccggt attaacggta    180
gtggcgctgg caaaagttgg cggcggtgtt gacagcctca gcacgccaat cggtaaagtc    240
gctggcgtgc tgctggcaac ggtttgttac ctggcggtgg ggccgctttt cgctacgccg    300
cgtacagcta ccgtttcctt tgaagtgggg attgcgccgc tgacgggtga ttccgcgctg    360
ccgctgttta tctacagcct ggtctatttc gctatcgtta ttctggtttc gctctatccg    420
ggcaagctgc tggataccgt gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc    480
atcctgtctg ttgccgcgat tgtctggccg gcgggttcta tcagcacggc gactgaggct    540
tatcaaaacg ctgcgttttc taacggcttc gttaacggct atctgaccat ggatacgctg    600
ggcgcaatgg tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa    660
gcgcgtctgc tgacccgtta taccgtctgg gctggcctga tggcgggtgt tggtctgact    720
ctgctgtacc tggcgctgtt ccgtctgggt tcagacagcg cgtcgctggt cgatcagtct    780
gcaaacggcg ctgctattct gcatgcttac gttcagcaca cctttggcgg cggcggtagc    840
ttcctgctgg cggcgttaat cttcatcgcc tgcctggtaa cggcagttgg cctgacctgt    900
gcttgtgcag aattctttgc ccagtacgta ccgctctctt atcgtacgct ggtgtttatc    960
ctcggcggct tctcgatggt ggtttctaac ctcggcttaa gccagctgat ccagatctcc   1020
gtaccggtgc tgaccgctat ttatccgccg tgtatcgcac tggttgtatt aagttttaca   1080
cgctcatggt ggcataattc gtcccgcgtg attgctccgc cgatgtttat cagcctgctt   1140
tttggtattc tcgacgggat caaagcatct gcattcagcg atatcttacc gtcctgggcg   1200
cagcgtttac cgctggccga acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg   1260
gttctggcca ttatctggga tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa   1320

<210> 2
<211> 439
<212> PRT
<213> Escherichia coli
<220>
<223> brnQ
<400> 2
Met Thr His Gln Leu Arg Ser Arg Asp Ile Ile Ala Leu Gly Phe Met
1               5                   10                  15
Thr Phe Ala Leu Phe Val Gly Ala Gly Asn Ile Ile Phe Pro Pro Met
            20                  25                  30
Val Gly Leu Gln Ala Gly Glu His Val Trp Thr Ala Ala Phe Gly Phe
        35                  40                  45
Leu Ile Thr Ala Val Gly Leu Pro Val Leu Thr Val Val Ala Leu Ala
    50                  55                  60
Lys Val Gly Gly Gly Val Asp Ser Leu Ser Thr Pro Ile Gly Lys Val
65                  70                  75                  80
Ala Gly Val Leu Leu Ala Thr Val Cys Tyr Leu Ala Val Gly Pro Leu
                85                  90                  95
Phe Ala Thr Pro Arg Thr Ala Thr Val Ser Phe Glu Val Gly Ile Ala
            100                 105                 110
Pro Leu Thr Gly Asp Ser Ala Leu Pro Leu Phe Ile Tyr Ser Leu Val
        115                 120                 125
Tyr Phe Ala Ile Val Ile Leu Val Ser Leu Tyr Pro Gly Lys Leu Leu
    130                 135                 140
Asp Thr Val Gly Asn Phe Leu Ala Pro Leu Lys Ile Ile Ala Leu Val
145                 150                 155                 160
Ile Leu Ser Val Ala Ala Ile Val Trp Pro Ala Gly Ser Ile Ser Thr
                165                 170                 175
Ala Thr Glu Ala Tyr Gln Asn Ala Ala Phe Ser Asn Gly Phe Val Asn
            180                 185                 190
Gly Tyr Leu Thr Met Asp Thr Leu Gly Ala Met Val Phe Gly Ile Val
        195                 200                 205
Ile Val Asn Ala Ala Arg Ser Arg Gly Val Thr Glu Ala Arg Leu Leu
    210                 215                 220
Thr Arg Tyr Thr Val Trp Ala Gly Leu Met Ala Gly Val Gly Leu Thr
225                 230                 235                 240
Leu Leu Tyr Leu Ala Leu Phe Arg Leu Gly Ser Asp Ser Ala Ser Leu
                245                 250                 255
Val Asp Gln Ser Ala Asn Gly Ala Ala Ile Leu His Ala Tyr Val Gln
            260                 265                 270
His Thr Phe Gly Gly Gly Gly Ser Phe Leu Leu Ala Ala Leu Ile Phe
        275                 280                 285
Ile Ala Cys Leu Val Thr Ala Val Gly Leu Thr Cys Ala Cys Ala Glu
    290                 295                 300
Phe Phe Ala Gln Tyr Val Pro Leu Ser Tyr Arg Thr Leu Val Phe Ile
305                 310                 315                 320
Leu Gly Gly Phe Ser Met Val Val Ser Asn Leu Gly Leu Ser Gln Leu
                325                 330                 335
Ile Gln Ile Ser Val Pro Val Leu Thr Ala Ile Tyr Pro Pro Cys Ile
            340                 345                 350
Ala Leu Val Val Leu Ser Phe Thr Arg Ser Trp Trp His Asn Ser Ser
        355                 360                 365
Arg Val Ile Ala Pro Pro Met Phe Ile Ser Leu Leu Phe Gly Ile Leu
    370                 375                 380
Asp Gly Ile Lys Ala Ser Ala Phe Ser Asp Ile Leu Pro Ser Trp Ala
385                 390                 395                 400
Gln Arg Leu Pro Leu Ala Glu Gln Gly Leu Ala Trp Leu Met Pro Thr
                405                 410                 415
Val Val Met Val Val Leu Ala Ile Ile Trp Asp Arg Ala Ala Gly Arg
            420                 425                 430
Gln Val Thr Ser Ser Ala His
        435

<210> 3
<211> 1223
<212> DNA
<213> Escherichia coli
<220>
<223> brnQ Mut
<400> 3
atgacccatc aattaagatc gcgcgatatc atcgctctgg gctttatgac atttgcgttg     60
ttcgtcggcg caggtaacat tattttccct ccaatggtcg gcttgcaggc aggcgaacac    120
gtctggactg cggcattcgg cttcctcatt actgccgttg gcctgccggt attaacggta    180
gtggcgctgg caaaagttgg cggcggtgtt gacagcctca gcacgccaat cggtaaagtc    240
gctggcgtgc tgctggcaac ggtttgttac ctggcggtgg ggccgctttt cgctacgccg    300
cgtacagcta ccgtttcctt tgaagtgggg attgcgccgc tgacgggtga ttccgcgctg    360
ccgctgttta tctacagcct ggtctatttc gctatcgtta ttctggtttc gctctatccg    420
ggcaagctgc tggataccgt gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc    480
atcctgtctg ttgccgcgat tgtctggccg gcgggttcta tcagcacggc gactgaggct    540
tatcaaaacg ctgcgttttc taacggcttc gttaacggct atctgaccat ggatacgctg    600
ggcgcaatgg tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa    660
gcgcgtcgct ggtcgatcag tctgcaaacg gcgctgctat tctgcatgct tacgttcagc    720
acacctttgg cggcggcggt agcttcctgc tggcggcgtt aatcttcatc gcctgcctgg    780
taacggcagt tggcctgacc tgtgcttgtg cagaattctt tgcccagtac gtaccgctct    840
cttatcgtac gctggtgttt atcctcggcg gcttctcgat ggtggtttct aacctcggct    900
taagccagct gatccagatc tccgtaccgg tgctgaccgc tatttatccg ccgtgtatcg    960
cactggttgt attaagtttt acacgctcat ggtggcataa ttcgtcccgc gtgattgctc   1020
cgccgatgtt tatcagcctg ctttttggta ttctcgacgg gatcaaagca tctgcattca   1080
gcgatatctt accgtcctgg gcgcagcgtt taccgctggc cgaacaaggt ctggcgtggt   1140
taatgccaac agtggtgatg gtggttctgg ccattatctg ggatcgcgcg gcaggtcgtc   1200
aggtgacctc cagcgctcac taa                                           1223

<210> 4
<211> 253
<212> PRT
<213> Escherichia coli
<220>
<223> brnQ_1 Mut1
<400> 4
Met Thr His Gln Leu Arg Ser Arg Asp Ile Ile Ala Leu Gly Phe Met
1               5                   10                  15
Thr Phe Ala Leu Phe Val Gly Ala Gly Asn Ile Ile Phe Pro Pro Met
            20                  25                  30
Val Gly Leu Gln Ala Gly Glu His Val Trp Thr Ala Ala Phe Gly Phe
        35                  40                  45
Leu Ile Thr Ala Val Gly Leu Pro Val Leu Thr Val Val Ala Leu Ala
    50                  55                  60
Lys Val Gly Gly Gly Val Asp Ser Leu Ser Thr Pro Ile Gly Lys Val
65                  70                  75                  80
Ala Gly Val Leu Leu Ala Thr Val Cys Tyr Leu Ala Val Gly Pro Leu
                85                  90                  95
Phe Ala Thr Pro Arg Thr Ala Thr Val Ser Phe Glu Val Gly Ile Ala
            100                 105                 110
Pro Leu Thr Gly Asp Ser Ala Leu Pro Leu Phe Ile Tyr Ser Leu Val
        115                 120                 125
Tyr Phe Ala Ile Val Ile Leu Val Ser Leu Tyr Pro Gly Lys Leu Leu
    130                 135                 140
Asp Thr Val Gly Asn Phe Leu Ala Pro Leu Lys Ile Ile Ala Leu Val
145                 150                 155                 160
Ile Leu Ser Val Ala Ala Ile Val Trp Pro Ala Gly Ser Ile Ser Thr
                165                 170                 175
Ala Thr Glu Ala Tyr Gln Asn Ala Ala Phe Ser Asn Gly Phe Val Asn
            180                 185                 190
Gly Tyr Leu Thr Met Asp Thr Leu Gly Ala Met Val Phe Gly Ile Val
        195                 200                 205
Ile Val Asn Ala Ala Arg Ser Arg Gly Val Thr Glu Ala Arg Arg Trp
    210                 215                 220
Ser Ile Ser Leu Gln Thr Ala Leu Leu Phe Cys Met Leu Thr Phe Ser
225                 230                 235                 240
Thr Pro Leu Ala Ala Ala Val Ala Ser Cys Trp Arg Arg
                245                 250

<210> 5
<211> 2283
<212> DNA
<213> Escherichia coli
<220>
<223> pflB
<400> 5
atgtccgagc ttaatgaaaa gttagccaca gcctgggaag gttttaccaa aggtgactgg     60
cagaatgaag taaacgtccg tgacttcatt cagaaaaact acactccgta cgagggtgac    120
gagtccttcc tggctggcgc tactgaagcg accaccaccc tgtgggacaa agtaatggaa    180
ggcgttaaac tggaaaaccg cactcacgcg ccagttgact ttgacaccgc tgttgcttcc    240
accatcacct ctcacgacgc tggctacatc aacaaagcgt tggaaaaagt tgttggtcta    300
cagactgaag ctccgctgaa acgtgctctt atcccgttcg gtggtatcaa aatgatcgag    360
ggttcctgca aagcgtacaa ccgcgaactg gacccgatga tcaaaaaaat cttcactgaa    420
taccgtaaaa ctcacaacca gggcgtgttc gacgtttaca ctccggacat cctgcgttgc    480
cgtaaatccg gtgttctgac cggtctgcca gatgcttatg gccgtggccg tatcatcggt    540
gactaccgtc gcgttgcgct gtacggtatc gactacctga tgaaagacaa atacgctcag    600
ttcacctctc tgcaggctga tctggaaaac ggcgtaaacc tggaacagac tatccgtctg    660
cgcgaagaaa tcgctgaaca gcaccgcgct ctgggtcaga tgaaagaaat ggctgcgaaa    720
tacggctacg acatctctgg tccggctacc aacgctcagg aagctatcca gtggacttac    780
ttcggctacc tggctgctgt taagtctcag aacggtgctg caatgtcctt cggtcgtacc    840
tccaccttcc tggatgtgta catcgaacgt gacctgaaag ctggcaagat caccgaacaa    900
gaagcgcagg aaatggttga ccacctggtc atgaaactgc gtatggttcg cttcctgcgt    960
actccggaat acgatgaact gttctctggc gacccgatct gggcaaccga atctatcggt   1020
ggtatgggcc tcgacggtcg taccctggtt accaaaaaca gcttccgttt cctgaacacc   1080
ctgtacacca tgggtccgtc tccggaaccg aacatgacca ttctgtggtc tgaaaaactg   1140
ccgctgaact tcaagaaatt cgccgctaaa gtgtccatcg acacctcttc tctgcaatat   1200
gagaacgatg acctgatgcg tccggacttc aacaacgatg actacgctat tgcttgctgc   1260
gtaagcccga tgatcgttgg taaacaaatg cagttcttcg gtgcgcgtgc aaacctggcg   1320
aaaaccatgc tgtacgcaat caacggcggc gttgacgaaa aactgaaaat gcaggttggt   1380
ccgaagtctg aaccgatcaa aggcgatgtc ctgaactatg atgaagtgat ggagcgcatg   1440
gatcacttca tggactggct ggctaaacag tacatcactg cactgaacat catccactac   1500
atgcacgaca agtacagcta cgaagcctct ctgatggcgc tgcacgaccg tgacgttatc   1560
cgcaccatgg cgtgtggtat cgctggtctg tccgttgctg ctgactccct gtctgcaatc   1620
aaatatgcga aagttaaacc gattcgtgac gaagacggtc tggctatcga cttcgaaatc   1680
gaaggcgaat acccgcagtt tggtaacaat gatccgcgtg tagatgacct ggctgttgac   1740
ctggtagaac gtttcatgaa gaaaattcag aaactgcaca cctaccgtga cgctatcccg   1800
actcagtctg ttctgaccat cacttctaac gttgtgtatg gtaagaaaac tggtaacacc   1860
ccagacggtc gtcgtgctgg cgcgccgttc ggaccgggtg ctaacccgat gcacggtcgt   1920
gaccagaaag gtgctgtagc gtctctgact tccgttgcta aactaccgtt tgcttacgct   1980
aaagatggta tctcctacac cttctctatc gttccgaacg cactgggtaa agacgacgaa   2040
gttcgtaaga ccaacctggc tggtctgatg gatggttact tccaccacga agcatccatc   2100
gaaggtggtc agcacctgaa cgttaacgtg atgaaccgtg aaatgctgct cgacgcgatg   2160
gaaaacccgg aaaaatatcc gcagctgacc atccgtgtat ctggctacgc agtacgtttc   2220
aactcgctga ctaaagaaca gcagcaggac gttattactc gtaccttcac tcaatctatg   2280
taa                                                                 2283

<210> 6
<211> 760
<212> PRT
<213> Escherichia coli
<220>
<223> pflB
<400> 6
Met Ser Glu Leu Asn Glu Lys Leu Ala Thr Ala Trp Glu Gly Phe Thr
1               5                   10                  15
Lys Gly Asp Trp Gln Asn Glu Val Asn Val Arg Asp Phe Ile Gln Lys
            20                  25                  30
Asn Tyr Thr Pro Tyr Glu Gly Asp Glu Ser Phe Leu Ala Gly Ala Thr
        35                  40                  45
Glu Ala Thr Thr Thr Leu Trp Asp Lys Val Met Glu Gly Val Lys Leu
    50                  55                  60
Glu Asn Arg Thr His Ala Pro Val Asp Phe Asp Thr Ala Val Ala Ser
65                  70                  75                  80
Thr Ile Thr Ser His Asp Ala Gly Tyr Ile Asn Lys Ala Leu Glu Lys
                85                  90                  95
Val Val Gly Leu Gln Thr Glu Ala Pro Leu Lys Arg Ala Leu Ile Pro
            100                 105                 110
Phe Gly Gly Ile Lys Met Ile Glu Gly Ser Cys Lys Ala Tyr Asn Arg
        115                 120                 125
Glu Leu Asp Pro Met Ile Lys Lys Ile Phe Thr Glu Tyr Arg Lys Thr
    130                 135                 140
His Asn Gln Gly Val Phe Asp Val Tyr Thr Pro Asp Ile Leu Arg Cys
145                 150                 155                 160
Arg Lys Ser Gly Val Leu Thr Gly Leu Pro Asp Ala Tyr Gly Arg Gly
                165                 170                 175
Arg Ile Ile Gly Asp Tyr Arg Arg Val Ala Leu Tyr Gly Ile Asp Tyr
            180                 185                 190
Leu Met Lys Asp Lys Tyr Ala Gln Phe Thr Ser Leu Gln Ala Asp Leu
        195                 200                 205
Glu Asn Gly Val Asn Leu Glu Gln Thr Ile Arg Leu Arg Glu Glu Ile
    210                 215                 220
Ala Glu Gln His Arg Ala Leu Gly Gln Met Lys Glu Met Ala Ala Lys
225                 230                 235                 240
Tyr Gly Tyr Asp Ile Ser Gly Pro Ala Thr Asn Ala Gln Glu Ala Ile
                245                 250                 255
Gln Trp Thr Tyr Phe Gly Tyr Leu Ala Ala Val Lys Ser Gln Asn Gly
            260                 265                 270
Ala Ala Met Ser Phe Gly Arg Thr Ser Thr Phe Leu Asp Val Tyr Ile
        275                 280                 285
Glu Arg Asp Leu Lys Ala Gly Lys Ile Thr Glu Gln Glu Ala Gln Glu
    290                 295                 300
Met Val Asp His Leu Val Met Lys Leu Arg Met Val Arg Phe Leu Arg
305                 310                 315                 320
Thr Pro Glu Tyr Asp Glu Leu Phe Ser Gly Asp Pro Ile Trp Ala Thr
                325                 330                 335
Glu Ser Ile Gly Gly Met Gly Leu Asp Gly Arg Thr Leu Val Thr Lys
            340                 345                 350
Asn Ser Phe Arg Phe Leu Asn Thr Leu Tyr Thr Met Gly Pro Ser Pro
        355                 360                 365
Glu Pro Asn Met Thr Ile Leu Trp Ser Glu Lys Leu Pro Leu Asn Phe
    370                 375                 380
Lys Lys Phe Ala Ala Lys Val Ser Ile Asp Thr Ser Ser Leu Gln Tyr
385                 390                 395                 400
Glu Asn Asp Asp Leu Met Arg Pro Asp Phe Asn Asn Asp Asp Tyr Ala
                405                 410                 415
Ile Ala Cys Cys Val Ser Pro Met Ile Val Gly Lys Gln Met Gln Phe
            420                 425                 430
Phe Gly Ala Arg Ala Asn Leu Ala Lys Thr Met Leu Tyr Ala Ile Asn
        435                 440                 445
Gly Gly Val Asp Glu Lys Leu Lys Met Gln Val Gly Pro Lys Ser Glu
    450                 455                 460
Pro Ile Lys Gly Asp Val Leu Asn Tyr Asp Glu Val Met Glu Arg Met
465                 470                 475                 480
Asp His Phe Met Asp Trp Leu Ala Lys Gln Tyr Ile Thr Ala Leu Asn
                485                 490                 495
Ile Ile His Tyr Met His Asp Lys Tyr Ser Tyr Glu Ala Ser Leu Met
            500                 505                 510
Ala Leu His Asp Arg Asp Val Ile Arg Thr Met Ala Cys Gly Ile Ala
        515                 520                 525
Gly Leu Ser Val Ala Ala Asp Ser Leu Ser Ala Ile Lys Tyr Ala Lys
    530                 535                 540
Val Lys Pro Ile Arg Asp Glu Asp Gly Leu Ala Ile Asp Phe Glu Ile
545                 550                 555                 560
Glu Gly Glu Tyr Pro Gln Phe Gly Asn Asn Asp Pro Arg Val Asp Asp
                565                 570                 575
Leu Ala Val Asp Leu Val Glu Arg Phe Met Lys Lys Ile Gln Lys Leu
            580                 585                 590
His Thr Tyr Arg Asp Ala Ile Pro Thr Gln Ser Val Leu Thr Ile Thr
        595                 600                 605
Ser Asn Val Val Tyr Gly Lys Lys Thr Gly Asn Thr Pro Asp Gly Arg
    610                 615                 620
Arg Ala Gly Ala Pro Phe Gly Pro Gly Ala Asn Pro Met His Gly Arg
625                 630                 635                 640
Asp Gln Lys Gly Ala Val Ala Ser Leu Thr Ser Val Ala Lys Leu Pro
                645                 650                 655
Phe Ala Tyr Ala Lys Asp Gly Ile Ser Tyr Thr Phe Ser Ile Val Pro
            660                 665                 670
Asn Ala Leu Gly Lys Asp Asp Glu Val Arg Lys Thr Asn Leu Ala Gly
        675                 680                 685
Leu Met Asp Gly Tyr Phe His His Glu Ala Ser Ile Glu Gly Gly Gln
    690                 695                 700
His Leu Asn Val Asn Val Met Asn Arg Glu Met Leu Leu Asp Ala Met
705                 710                 715                 720
Glu Asn Pro Glu Lys Tyr Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr
                725                 730                 735
Ala Val Arg Phe Asn Ser Leu Thr Lys Glu Gln Gln Gln Asp Val Ile
            740                 745                 750
Thr Arg Thr Phe Thr Gln Ser Met
        755                 760

<210> 7
<211> 2676
<212> DNA
<213> Escherichia coli
<220>
<223> adhE
<400> 7
atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag     60
cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg    120
gctgctgcag atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt    180
atcgtcgaag ataaagtgat caaaaaccac tttgcttctg aatatatcta caacgcctat    240
aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc    300
gctgaaccaa tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaactgct    360
atcttcaaat cgctgatcag tctgaagacc cgtaacgcca ttatcttctc cccgcacccg    420
cgtgcaaaag atgccaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc    480
ggtgctccga aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca    540
ctgatgcacc acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa    600
gccgcataca gctccggtaa accagctatc ggtgtaggcg cgggcaacac tccagttgtt    660
atcgatgaaa ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc    720
gacaacggcg taatctgtgc ttctgaacag tctgttgttg ttgttgactc tgtttatgac    780
gctgtacgtg aacgttttgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa    840
gctgttcagg atgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca    900
gcctataaaa ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc    960
ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact   1020
ctggcaatgt accgcgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt   1080
gctatgggcg gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct   1140
cgcgtttctt acttcggtca gaaaatgaaa acggctcgta tcctgattaa caccccagcg   1200
tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt   1260
tgtggttctt ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac   1320
aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc   1380
tacttccgcc gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa   1440
cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg gttatgctga tcagatcact   1500
tccgtactga aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agcggacccg   1560
accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt   1620
atcgcgctgg gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa   1680
catccggaaa ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc   1740
tacaagttcc cgaaaatggg cgtgaaagcg aaaatgatcg ctgtcaccac cacttctggt   1800
acaggttctg aagtcactcc gtttgcggtt gtaactgacg acgctactgg tcagaaatat   1860
ccgctggcag actatgcgct gactccggat atggcgattg tcgacgccaa cctggttatg   1920
gacatgccga agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa   1980
gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa   2040
ctgctgaaag aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt   2100
gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt   2160
gtatgtcact caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca   2220
aacgccctgc tgatttgtaa cgttattcgc tacaatgcga acgacaaccc gaccaagcag   2280
actgcattca gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgccgac   2340
cacttgggtc tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca   2400
tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga agctggcgtt   2460
caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcgtt cgatgaccag   2520
tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat cctgctggat   2580
acctactacg gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga agccgctccg   2640
gctaaagctg agaaaaaagc gaaaaaatcc gcttaa                             2676

<210> 8
<211> 891
<212> PRT
<213> Escherichia coli
<220>
<223> adhE
<400> 8
Met Ala Val Thr Asn Val Ala Glu Leu Asn Ala Leu Val Glu Arg Val
1               5                   10                  15
Lys Lys Ala Gln Arg Glu Tyr Ala Ser Phe Thr Gln Glu Gln Val Asp
            20                  25                  30
Lys Ile Phe Arg Ala Ala Ala Leu Ala Ala Ala Asp Ala Arg Ile Pro
        35                  40                  45
Leu Ala Lys Met Ala Val Ala Glu Ser Gly Met Gly Ile Val Glu Asp
    50                  55                  60
Lys Val Ile Lys Asn His Phe Ala Ser Glu Tyr Ile Tyr Asn Ala Tyr
65                  70                  75                  80
Lys Asp Glu Lys Thr Cys Gly Val Leu Ser Glu Asp Asp Thr Phe Gly
                85                  90                  95
Thr Ile Thr Ile Ala Glu Pro Ile Gly Ile Ile Cys Gly Ile Val Pro
            100                 105                 110
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu
        115                 120                 125
Lys Thr Arg Asn Ala Ile Ile Phe Ser Pro His Pro Arg Ala Lys Asp
    130                 135                 140
Ala Thr Asn Lys Ala Ala Asp Ile Val Leu Gln Ala Ala Ile Ala Ala
145                 150                 155                 160
Gly Ala Pro Lys Asp Leu Ile Gly Trp Ile Asp Gln Pro Ser Val Glu
                165                 170                 175
Leu Ser Asn Ala Leu Met His His Pro Asp Ile Asn Leu Ile Leu Ala
            180                 185                 190
Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro
        195                 200                 205
Ala Ile Gly Val Gly Ala Gly Asn Thr Pro Val Val Ile Asp Glu Thr
    210                 215                 220
Ala Asp Ile Lys Arg Ala Val Ala Ser Val Leu Met Ser Lys Thr Phe
225                 230                 235                 240
Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser Val Val Val Val Asp
                245                 250                 255
Ser Val Tyr Asp Ala Val Arg Glu Arg Phe Ala Thr His Gly Gly Tyr
            260                 265                 270
Leu Leu Gln Gly Lys Glu Leu Lys Ala Val Gln Asp Val Ile Leu Lys
        275                 280                 285
Asn Gly Ala Leu Asn Ala Ala Ile Val Gly Gln Pro Ala Tyr Lys Ile
    290                 295                 300
Ala Glu Leu Ala Gly Phe Ser Val Pro Glu Asn Thr Lys Ile Leu Ile
305                 310                 315                 320
Gly Glu Val Thr Val Val Asp Glu Ser Glu Pro Phe Ala His Glu Lys
                325                 330                 335
Leu Ser Pro Thr Leu Ala Met Tyr Arg Ala Lys Asp Phe Glu Asp Ala
            340                 345                 350
Val Glu Lys Ala Glu Lys Leu Val Ala Met Gly Gly Ile Gly His Thr
        355                 360                 365
Ser Cys Leu Tyr Thr Asp Gln Asp Asn Gln Pro Ala Arg Val Ser Tyr
    370                 375                 380
Phe Gly Gln Lys Met Lys Thr Ala Arg Ile Leu Ile Asn Thr Pro Ala
385                 390                 395                 400
Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn Phe Lys Leu Ala Pro Ser
                405                 410                 415
Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Ser Glu Asn
            420                 425                 430
Val Gly Pro Lys His Leu Ile Asn Lys Lys Thr Val Ala Lys Arg Ala
        435                 440                 445
Glu Asn Met Leu Trp His Lys Leu Pro Lys Ser Ile Tyr Phe Arg Arg
    450                 455                 460
Gly Ser Leu Pro Ile Ala Leu Asp Glu Val Ile Thr Asp Gly His Lys
465                 470                 475                 480
Arg Ala Leu Ile Val Thr Asp Arg Phe Leu Phe Asn Asn Gly Tyr Ala
                485                 490                 495
Asp Gln Ile Thr Ser Val Leu Lys Ala Ala Gly Val Glu Thr Glu Val
            500                 505                 510
Phe Phe Glu Val Glu Ala Asp Pro Thr Leu Ser Ile Val Arg Lys Gly
        515                 520                 525
Ala Glu Leu Ala Asn Ser Phe Lys Pro Asp Val Ile Ile Ala Leu Gly
    530                 535                 540
Gly Gly Ser Pro Met Asp Ala Ala Lys Ile Met Trp Val Met Tyr Glu
545                 550                 555                 560
His Pro Glu Thr His Phe Glu Glu Leu Ala Leu Arg Phe Met Asp Ile
                565                 570                 575
Arg Lys Arg Ile Tyr Lys Phe Pro Lys Met Gly Val Lys Ala Lys Met
            580                 585                 590
Ile Ala Val Thr Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe
        595                 600                 605
Ala Val Val Thr Asp Asp Ala Thr Gly Gln Lys Tyr Pro Leu Ala Asp
    610                 615                 620
Tyr Ala Leu Thr Pro Asp Met Ala Ile Val Asp Ala Asn Leu Val Met
625                 630                 635                 640
Asp Met Pro Lys Ser Leu Cys Ala Phe Gly Gly Leu Asp Ala Val Thr
                645                 650                 655
His Ala Met Glu Ala Tyr Val Ser Val Leu Ala Ser Glu Phe Ser Asp
            660                 665                 670
Gly Gln Ala Leu Gln Ala Leu Lys Leu Leu Lys Glu Tyr Leu Pro Ala
        675                 680                 685
Ser Tyr His Glu Gly Ser Lys Asn Pro Val Ala Arg Glu Arg Val His
    690                 695                 700
Ser Ala Ala Thr Ile Ala Gly Ile Ala Phe Ala Asn Ala Phe Leu Gly
705                 710                 715                 720
Val Cys His Ser Met Ala His Lys Leu Gly Ser Gln Phe His Ile Pro
                725                 730                 735
His Gly Leu Ala Asn Ala Leu Leu Ile Cys Asn Val Ile Arg Tyr Asn
            740                 745                 750
Ala Asn Asp Asn Pro Thr Lys Gln Thr Ala Phe Ser Gln Tyr Asp Arg
        755                 760                 765
Pro Gln Ala Arg Arg Arg Tyr Ala Glu Ile Ala Asp His Leu Gly Leu
    770                 775                 780
Ser Ala Pro Gly Asp Arg Thr Ala Ala Lys Ile Glu Lys Leu Leu Ala
785                 790                 795                 800
Trp Leu Glu Thr Leu Lys Ala Glu Leu Gly Ile Pro Lys Ser Ile Arg
                805                 810                 815
Glu Ala Gly Val Gln Glu Ala Asp Phe Leu Ala Asn Val Asp Lys Leu
            820                 825                 830
Ser Glu Asp Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr
        835                 840                 845
Pro Leu Ile Ser Glu Leu Lys Gln Ile Leu Leu Asp Thr Tyr Tyr Gly
    850                 855                 860
Arg Asp Tyr Val Glu Gly Glu Thr Ala Ala Lys Lys Glu Ala Ala Pro
865                 870                 875                 880
Ala Lys Ala Glu Lys Lys Ala Lys Lys Ser Ala
                885                 890

<210> 9
<211> 990
<212> DNA
<213> Escherichia coli
<220>
<223> ldhA
<400> 9
atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca acaggtgaac     60
gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa aaccgctaaa    120
actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag ccgcccggtg    180
ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc cggtttcaat    240
aacgtcgacc ttgacgcggc aaaagaactg gggctgaaag tagtccgtgt tccagcctat    300
gatccagagg ccgttgctga acacgccatc ggtatgatga tgacgctgaa ccgccgtatt    360
caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct gaccggcttt    420
actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt ggcgatgctg    480
cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc aagtgcagcg    540
gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga atcagacgtt    600
atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga agccgccttc    660
gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt gattgattct    720
caggcagcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat ggacgtgtat    780
gagaacgaac gcgatctatt ctttgaagat aaatccaacg acgtaattca ggatgacgta    840
ttccgtcgcc tgtctgcctg ccacaacgtg ctatttaccg ggcaccaggc attcctgaca    900
gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa tctggaaaaa    960
ggcgaaacct gcccgaacga actggtttaa                                     990

<210> 10
<211> 329
<212> PRT
<213> Escherichia coli
<220>
<223> ldhA
<400> 10
Met Lys Leu Ala Val Tyr Ser Thr Lys Gln Tyr Asp Lys Lys Tyr Leu
1               5                   10                  15
Gln Gln Val Asn Glu Ser Phe Gly Phe Glu Leu Glu Phe Phe Asp Phe
            20                  25                  30
Leu Leu Thr Glu Lys Thr Ala Lys Thr Ala Asn Gly Cys Glu Ala Val
        35                  40                  45
Cys Ile Phe Val Asn Asp Asp Gly Ser Arg Pro Val Leu Glu Glu Leu
    50                  55                  60
Lys Lys His Gly Val Lys Tyr Ile Ala Leu Arg Cys Ala Gly Phe Asn
65                  70                  75                  80
Asn Val Asp Leu Asp Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg
                85                  90                  95
Val Pro Ala Tyr Asp Pro Glu Ala Val Ala Glu His Ala Ile Gly Met
            100                 105                 110
Met Met Thr Leu Asn Arg Arg Ile His Arg Ala Tyr Gln Arg Thr Arg
        115                 120                 125
Asp Ala Asn Phe Ser Leu Glu Gly Leu Thr Gly Phe Thr Met Tyr Gly
    130                 135                 140
Lys Thr Ala Gly Val Ile Gly Thr Gly Lys Ile Gly Val Ala Met Leu
145                 150                 155                 160
Arg Ile Leu Lys Gly Phe Gly Met Arg Leu Leu Ala Phe Asp Pro Tyr
                165                 170                 175
Pro Ser Ala Ala Ala Leu Glu Leu Gly Val Glu Tyr Val Asp Leu Pro
            180                 185                 190
Thr Leu Phe Ser Glu Ser Asp Val Ile Ser Leu His Cys Pro Leu Thr
        195                 200                 205
Pro Glu Asn Tyr His Leu Leu Asn Glu Ala Ala Phe Asp Gln Met Lys
    210                 215                 220
Asn Gly Val Met Ile Val Asn Thr Ser Arg Gly Ala Leu Ile Asp Ser
225                 230                 235                 240
Gln Ala Ala Ile Glu Ala Leu Lys Asn Gln Lys Ile Gly Ser Leu Gly
                245                 250                 255
Met Asp Val Tyr Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser
            260                 265                 270
Asn Asp Val Ile Gln Asp Asp Val Phe Arg Arg Leu Ser Ala Cys His
        275                 280                 285
Asn Val Leu Phe Thr Gly His Gln Ala Phe Leu Thr Ala Glu Ala Leu
    290                 295                 300
Thr Ser Ile Ser Gln Thr Thr Leu Gln Asn Leu Ser Asn Leu Glu Lys
305                 310                 315                 320
Gly Glu Thr Cys Pro Asn Glu Leu Val
                325

<210> 11
<211> 2145
<212> DNA
<213> Escherichia coli
<220>
<223> pta
<400> 11
gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc     60
cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc    120
gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac    180
tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc    240
agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa    300
gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag    360
tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag    420
ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc    480
ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat    540
gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa    600
gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct    660
gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat    720
gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc    780
gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc    840
gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc    900
ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa    960
cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct   1020
ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa   1080
gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact   1140
tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac tgaacttgcg   1200
cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca   1260
gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag   1320
atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga aatcgttgat   1380
ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc   1440
atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg   1500
ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc   1560
atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg   1620
ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat   1680
ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc   1740
ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc   1800
gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg   1860
atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg   1920
ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt   1980
aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg   2040
cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc   2100
tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaa                   2145

<210> 12
<211> 714
<212> PRT
<213> Escherichia coli
<220>
<223> pta
<400> 12
Met Ser Arg Ile Ile Met Leu Ile Pro Thr Gly Thr Ser Val Gly Leu
1               5                   10                  15
Thr Ser Val Ser Leu Gly Val Ile Arg Ala Met Glu Arg Lys Gly Val
            20                  25                  30
Arg Leu Ser Val Phe Lys Pro Ile Ala Gln Pro Arg Thr Gly Gly Asp
        35                  40                  45
Ala Pro Asp Gln Thr Thr Thr Ile Val Arg Ala Asn Ser Ser Thr Thr
    50                  55                  60
Thr Ala Ala Glu Pro Leu Lys Met Ser Tyr Val Glu Gly Leu Leu Ser
65                  70                  75                  80
Ser Asn Gln Lys Asp Val Leu Met Glu Glu Ile Val Ala Asn Tyr His
                85                  90                  95
Ala Asn Thr Lys Asp Ala Glu Val Val Leu Val Glu Gly Leu Val Pro
            100                 105                 110
Thr Arg Lys His Gln Phe Ala Gln Ser Leu Asn Tyr Glu Ile Ala Lys
        115                 120                 125
Thr Leu Asn Ala Glu Ile Val Phe Val Met Ser Gln Gly Thr Asp Thr
    130                 135                 140
Pro Glu Gln Leu Lys Glu Arg Ile Glu Leu Thr Arg Asn Ser Phe Gly
145                 150                 155                 160
Gly Ala Lys Asn Thr Asn Ile Thr Gly Val Ile Val Asn Lys Leu Asn
                165                 170                 175
Ala Pro Val Asp Glu Gln Gly Arg Thr Arg Pro Asp Leu Ser Glu Ile
            180                 185                 190
Phe Asp Asp Ser Ser Lys Ala Lys Val Asn Asn Val Asp Pro Ala Lys
        195                 200                 205
Leu Gln Glu Ser Ser Pro Leu Pro Val Leu Gly Ala Val Pro Trp Ser
    210                 215                 220
Phe Asp Leu Ile Ala Thr Arg Ala Ile Asp Met Ala Arg His Leu Asn
225                 230                 235                 240
Ala Thr Ile Ile Asn Glu Gly Asp Ile Asn Thr Arg Arg Val Lys Ser
                245                 250                 255
Val Thr Phe Cys Ala Arg Ser Ile Pro His Met Leu Glu His Phe Arg
            260                 265                 270
Ala Gly Ser Leu Leu Val Thr Ser Ala Asp Arg Pro Asp Val Leu Val
        275                 280                 285
Ala Ala Cys Leu Ala Ala Met Asn Gly Val Glu Ile Gly Ala Leu Leu
    290                 295                 300
Leu Thr Gly Gly Tyr Glu Met Asp Ala Arg Ile Ser Lys Leu Cys Glu
305                 310                 315                 320
Arg Ala Phe Ala Thr Gly Leu Pro Val Phe Met Val Asn Thr Asn Thr
                325                 330                 335
Trp Gln Thr Ser Leu Ser Leu Gln Ser Phe Asn Leu Glu Val Pro Val
            340                 345                 350
Asp Asp His Glu Arg Ile Glu Lys Val Gln Glu Tyr Val Ala Asn Tyr
        355                 360                 365
Ile Asn Ala Asp Trp Ile Glu Ser Leu Thr Ala Thr Ser Glu Arg Ser
    370                 375                 380
Arg Arg Leu Ser Pro Pro Ala Phe Arg Tyr Gln Leu Thr Glu Leu Ala
385                 390                 395                 400
Arg Lys Ala Gly Lys Arg Ile Val Leu Pro Glu Gly Asp Glu Pro Arg
                405                 410                 415
Thr Val Lys Ala Ala Ala Ile Cys Ala Glu Arg Gly Ile Ala Thr Cys
            420                 425                 430
Val Leu Leu Gly Asn Pro Ala Glu Ile Asn Arg Val Ala Ala Ser Gln
        435                 440                 445
Gly Val Glu Leu Gly Ala Gly Ile Glu Ile Val Asp Pro Glu Val Val
    450                 455                 460
Arg Glu Ser Tyr Val Gly Arg Leu Val Glu Leu Arg Lys Asn Lys Gly
465                 470                 475                 480
Met Thr Glu Thr Val Ala Arg Glu Gln Leu Glu Asp Asn Val Val Leu
                485                 490                 495
Gly Thr Leu Met Leu Glu Gln Asp Glu Val Asp Gly Leu Val Ser Gly
            500                 505                 510
Ala Val His Thr Thr Ala Asn Thr Ile Arg Pro Pro Leu Gln Leu Ile
        515                 520                 525
Lys Thr Ala Pro Gly Ser Ser Leu Val Ser Ser Val Phe Phe Met Leu
    530                 535                 540
Leu Pro Glu Gln Val Tyr Val Tyr Gly Asp Cys Ala Ile Asn Pro Asp
545                 550                 555                 560
Pro Thr Ala Glu Gln Leu Ala Glu Ile Ala Ile Gln Ser Ala Asp Ser
                565                 570                 575
Ala Ala Ala Phe Gly Ile Glu Pro Arg Val Ala Met Leu Ser Tyr Ser
            580                 585                 590
Thr Gly Thr Ser Gly Ala Gly Ser Asp Val Glu Lys Val Arg Glu Ala
        595                 600                 605
Thr Arg Leu Ala Gln Glu Lys Arg Pro Asp Leu Met Ile Asp Gly Pro
    610                 615                 620
Leu Gln Tyr Asp Ala Ala Val Met Ala Asp Val Ala Lys Ser Lys Ala
625                 630                 635                 640
Pro Asn Ser Pro Val Ala Gly Arg Ala Thr Val Phe Ile Phe Pro Asp
                645                 650                 655
Leu Asn Thr Gly Asn Thr Thr Tyr Lys Ala Val Gln Arg Ser Ala Asp
            660                 665                 670
Leu Ile Ser Ile Gly Pro Met Leu Gln Gly Met Arg Lys Pro Val Asn
        675                 680                 685
Asp Leu Ser Arg Gly Ala Leu Val Asp Asp Ile Val Tyr Thr Ile Ala
    690                 695                 700
Leu Thr Ala Ile Gln Ser Ala Gln Gln Gln
705                 710

<210> 13
<211> 1809
<212> DNA
<213> Escherichia coli
<220>
<223> frdA
<400> 13
gtgcaaacct ttcaagccga tcttgccatt gtaggcgccg gtggcgcggg attacgtgct     60
gcaattgctg ccgcgcaggc aaatccgaat gcaaaaatcg cactaatctc aaaagtatac    120
ccgatgcgta gccataccgt tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat    180
gacagcttcg aatatcactt tcacgataca gtagcgggtg gcgactggtt gtgtgagcag    240
gatgtcgtgg attatttcgt ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg    300
ggatgcccat ggagccgtcg cccggatggt agcgtcaacg tacgtcgctt cggcggcatg    360
aaaatcgagc gcacctggtt cgccgccgat aagaccggct tccatatgct gcacacgctg    420
ttccagacct ctctgcaatt cccgcagatc cagcgttttg acgaacattt cgtgctggat    480
attctggttg atgatggtca tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg    540
ctggtgcaga tccgtgctaa cgcggtcgtt atggctactg gcggtgcggg tcgcgtttat    600
cgttacaaca ccaacggcgg catcgttacc ggtgacggta tgggtatggc gctaagccac    660
ggcgttccgc tgcgtgacat ggaattcgtt cagtatcacc caaccggtct gccaggttcc    720
ggtatcctga tgaccgaagg ctgccgcggt gaaggcggta ttctggtcaa caaaaatggc    780
taccgttatc tgcaagatta cggcatgggc ccggaaactc cgctgggcga gccgaaaaac    840
aaatatatgg aactgggtcc acgcgacaaa gtttctcagg ccttctggca cgaatggcgt    900
aaaggcaaca ccatctccac gccgcgtggc gatgtggttt atctcgacct gcgtcacctc    960
ggcgagaaaa aactgcatga acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt   1020
ggcgtcgatc cggttaaaga accgattccg gtacgtccga ccgcacacta caccatgggc   1080
ggtatcgaaa ccgatcagaa ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa   1140
tgttcctctg ttggtctgca cggtgcaaac cgtctgggtt ctaactccct ggcggaactg   1200
gtggtcttcg gccgtctggc cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat   1260
ggcaacgaag cggcaattga agcgcaggca gctggcgttg aacaacgtct gaaagatctg   1320
gttaaccagg atggcggcga aaactgggcg aagatccgcg acgaaatggg cctggcaatg   1380
gaagaaggtt gcggtatcta ccgtacgccg gaactgatgc agaaaaccat cgacaagctg   1440
gcagagctgc aggaacgctt caagcgcgtg cgcatcaccg acacctccag cgtgttcaac   1500
accgacctgc tctacaccat tgaactgggc cacggtctga acgttgctga atgtatggcg   1560
cactccgcaa tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc   1620
accgagcgtg acgacgtcaa cttcctcaaa cacaccctcg ccttccgcga tgctgatggc   1680
acgactcgcc tggagtacag cgacgtgaag attactacgc tgccgccagc taaacgcgtt   1740
tacggtggcg aagcggatgc agccgataag gcggaagcag ccaataagaa ggagaaggcg   1800
aatggctga                                                           1809

<210> 14
<211> 602
<212> PRT
<213> Escherichia coli
<220>
<223> frdA
<400> 14
Met Gln Thr Phe Gln Ala Asp Leu Ala Ile Val Gly Ala Gly Gly Ala
1               5                   10                  15
Gly Leu Arg Ala Ala Ile Ala Ala Ala Gln Ala Asn Pro Asn Ala Lys
            20                  25                  30
Ile Ala Leu Ile Ser Lys Val Tyr Pro Met Arg Ser His Thr Val Ala
        35                  40                  45
Ala Glu Gly Gly Ser Ala Ala Val Ala Gln Asp His Asp Ser Phe Glu
    50                  55                  60
Tyr His Phe His Asp Thr Val Ala Gly Gly Asp Trp Leu Cys Glu Gln
65                  70                  75                  80
Asp Val Val Asp Tyr Phe Val His His Cys Pro Thr Glu Met Thr Gln
                85                  90                  95
Leu Glu Leu Trp Gly Cys Pro Trp Ser Arg Arg Pro Asp Gly Ser Val
            100                 105                 110
Asn Val Arg Arg Phe Gly Gly Met Lys Ile Glu Arg Thr Trp Phe Ala
        115                 120                 125
Ala Asp Lys Thr Gly Phe His Met Leu His Thr Leu Phe Gln Thr Ser
    130                 135                 140
Leu Gln Phe Pro Gln Ile Gln Arg Phe Asp Glu His Phe Val Leu Asp
145                 150                 155                 160
Ile Leu Val Asp Asp Gly His Val Arg Gly Leu Val Ala Met Asn Met
                165                 170                 175
Met Glu Gly Thr Leu Val Gln Ile Arg Ala Asn Ala Val Val Met Ala
            180                 185                 190
Thr Gly Gly Ala Gly Arg Val Tyr Arg Tyr Asn Thr Asn Gly Gly Ile
        195                 200                 205
Val Thr Gly Asp Gly Met Gly Met Ala Leu Ser His Gly Val Pro Leu
    210                 215                 220
Arg Asp Met Glu Phe Val Gln Tyr His Pro Thr Gly Leu Pro Gly Ser
225                 230                 235                 240
Gly Ile Leu Met Thr Glu Gly Cys Arg Gly Glu Gly Gly Ile Leu Val
                245                 250                 255
Asn Lys Asn Gly Tyr Arg Tyr Leu Gln Asp Tyr Gly Met Gly Pro Glu
            260                 265                 270
Thr Pro Leu Gly Glu Pro Lys Asn Lys Tyr Met Glu Leu Gly Pro Arg
        275                 280                 285
Asp Lys Val Ser Gln Ala Phe Trp His Glu Trp Arg Lys Gly Asn Thr
    290                 295                 300
Ile Ser Thr Pro Arg Gly Asp Val Val Tyr Leu Asp Leu Arg His Leu
305                 310                 315                 320
Gly Glu Lys Lys Leu His Glu Arg Leu Pro Phe Ile Cys Glu Leu Ala
                325                 330                 335
Lys Ala Tyr Val Gly Val Asp Pro Val Lys Glu Pro Ile Pro Val Arg
            340                 345                 350
Pro Thr Ala His Tyr Thr Met Gly Gly Ile Glu Thr Asp Gln Asn Cys
        355                 360                 365
Glu Thr Arg Ile Lys Gly Leu Phe Ala Val Gly Glu Cys Ser Ser Val
    370                 375                 380
Gly Leu His Gly Ala Asn Arg Leu Gly Ser Asn Ser Leu Ala Glu Leu
385                 390                 395                 400
Val Val Phe Gly Arg Leu Ala Gly Glu Gln Ala Thr Glu Arg Ala Ala
                405                 410                 415
Thr Ala Gly Asn Gly Asn Glu Ala Ala Ile Glu Ala Gln Ala Ala Gly
            420                 425                 430
Val Glu Gln Arg Leu Lys Asp Leu Val Asn Gln Asp Gly Gly Glu Asn
        435                 440                 445
Trp Ala Lys Ile Arg Asp Glu Met Gly Leu Ala Met Glu Glu Gly Cys
    450                 455                 460
Gly Ile Tyr Arg Thr Pro Glu Leu Met Gln Lys Thr Ile Asp Lys Leu
465                 470                 475                 480
Ala Glu Leu Gln Glu Arg Phe Lys Arg Val Arg Ile Thr Asp Thr Ser
                485                 490                 495
Ser Val Phe Asn Thr Asp Leu Leu Tyr Thr Ile Glu Leu Gly His Gly
            500                 505                 510
Leu Asn Val Ala Glu Cys Met Ala His Ser Ala Met Ala Arg Lys Glu
        515                 520                 525
Ser Arg Gly Ala His Gln Arg Leu Asp Glu Gly Cys Thr Glu Arg Asp
    530                 535                 540
Asp Val Asn Phe Leu Lys His Thr Leu Ala Phe Arg Asp Ala Asp Gly
545                 550                 555                 560
Thr Thr Arg Leu Glu Tyr Ser Asp Val Lys Ile Thr Thr Leu Pro Pro
                565                 570                 575
Ala Lys Arg Val Tyr Gly Gly Glu Ala Asp Ala Ala Asp Lys Ala Glu
            580                 585                 590
Ala Ala Asn Lys Lys Glu Lys Ala Asn Gly
        595                 600

<210> 15
<211> 1119
<212> DNA
<213> G. stearothermophilus
<220>
<223> alaD
<400> 15
atgaaaattg gcatccctaa agagattaag aacaatgaaa accgtgtagc aatcaccccg     60
gcaggtgtta tgactctggt taaagcgggc cacgatgtgt acgtcgaaac cgaagcgggt    120
gccggcagcg gcttcagcga cagcgagtat gagaaggcgg gtgcggttat tgtgactaag    180
gcggaggacg cttgggcagc cgaaatggtt ctgaaggtga aagaaccgct ggcggaggag    240
tttcgctatt ttcgtccggg tctgattttg ttcacctacc tgcacctggc tgcggccgag    300
gcgctgacca aggcactggt ggagcagaag gttgttggca tcgcgtacga aacggttcaa    360
ctggcgaatg gttccctgcc gctgctgacc cctatgtctg aagttgcggg tcgcatgagc    420
gttcaagtcg gcgctcagtt tctggagaaa ccgcacggtg gcaagggcat tttgctgggt    480
ggtgttccgg gtgtccgccg tggtaaagtg acgatcattg gcggtggtac ggccggtacg    540
aacgcggcca agattgccgt aggtctgggt gcagatgtga ccattctgga catcaacgcg    600
gaacgtttgc gtgagctgga cgacctgttt ggcgaccaag tcaccaccct gatgagcaac    660
agctaccaca tcgcggagtg cgtccgtgaa agcgatttgg tcgttggtgc ggtgctgatc    720
ccgggtgcaa aagccccgaa actggtgacc gaggagatgg tccgtagcat gaccccgggt    780
tcggttctgg tcgacgtggc aattgaccag ggcggtatct tcgaaaccac cgaccgcgtc    840
acgacccatg atgacccgac ctatgtgaaa catggcgtgg ttcactatgc ggtcgcgaat    900
atgccgggtg cagtgccgcg cacgtccacg ttcgcgctga cgaacgtgac gattccatac    960
gctctgcaga tcgccaataa gggctatcgt gcggcgtgtc tggataatcc ggcattgctg   1020
aaaggcatca ataccctgga tggtcatatc gtttacgagg ctgtggctgc agcacacaac   1080
atgccgtaca ctgatgtcca tagcttgctg caaggctaa                          1119

<210> 16
<211> 372
<212> PRT
<213> G. stearothermophilus
<220>
<223> alaD
<400> 16
Met Lys Ile Gly Ile Pro Lys Glu Ile Lys Asn Asn Glu Asn Arg Val
1               5                   10                  15
Ala Ile Thr Pro Ala Gly Val Met Thr Leu Val Lys Ala Gly His Asp
            20                  25                  30
Val Tyr Val Glu Thr Glu Ala Gly Ala Gly Ser Gly Phe Ser Asp Ser
        35                  40                  45
Glu Tyr Glu Lys Ala Gly Ala Val Ile Val Thr Lys Ala Glu Asp Ala
    50                  55                  60
Trp Ala Ala Glu Met Val Leu Lys Val Lys Glu Pro Leu Ala Glu Glu
65                  70                  75                  80
Phe Arg Tyr Phe Arg Pro Gly Leu Ile Leu Phe Thr Tyr Leu His Leu
                85                  90                  95
Ala Ala Ala Glu Ala Leu Thr Lys Ala Leu Val Glu Gln Lys Val Val
            100                 105                 110
Gly Ile Ala Tyr Glu Thr Val Gln Leu Ala Asn Gly Ser Leu Pro Leu
        115                 120                 125
Leu Thr Pro Met Ser Glu Val Ala Gly Arg Met Ser Val Gln Val Gly
    130                 135                 140
Ala Gln Phe Leu Glu Lys Pro His Gly Gly Lys Gly Ile Leu Leu Gly
145                 150                 155                 160
Gly Val Pro Gly Val Arg Arg Gly Lys Val Thr Ile Ile Gly Gly Gly
                165                 170                 175
Thr Ala Gly Thr Asn Ala Ala Lys Ile Ala Val Gly Leu Gly Ala Asp
            180                 185                 190
Val Thr Ile Leu Asp Ile Asn Ala Glu Arg Leu Arg Glu Leu Asp Asp
        195                 200                 205
Leu Phe Gly Asp Gln Val Thr Thr Leu Met Ser Asn Ser Tyr His Ile
    210                 215                 220
Ala Glu Cys Val Arg Glu Ser Asp Leu Val Val Gly Ala Val Leu Ile
225                 230                 235                 240
Pro Gly Ala Lys Ala Pro Lys Leu Val Thr Glu Glu Met Val Arg Ser
                245                 250                 255
Met Thr Pro Gly Ser Val Leu Val Asp Val Ala Ile Asp Gln Gly Gly
            260                 265                 270
Ile Phe Glu Thr Thr Asp Arg Val Thr Thr His Asp Asp Pro Thr Tyr
        275                 280                 285
Val Lys His Gly Val Val His Tyr Ala Val Ala Asn Met Pro Gly Ala
    290                 295                 300
Val Pro Arg Thr Ser Thr Phe Ala Leu Thr Asn Val Thr Ile Pro Tyr
305                 310                 315                 320
Ala Leu Gln Ile Ala Asn Lys Gly Tyr Arg Ala Ala Cys Leu Asp Asn
                325                 330                 335
Pro Ala Leu Leu Lys Gly Ile Asn Thr Leu Asp Gly His Ile Val Tyr
            340                 345                 350
Glu Ala Val Ala Ala Ala His Asn Met Pro Tyr Thr Asp Val His Ser
        355                 360                 365
Leu Leu Gln Gly
    370

<210> 17
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check1
<400> 17
actgcggtag ttcttcactg                                                 20

<210> 18
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check2
<400> 18
agtacctttc tggtttagcc g                                               21

<210> 19
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check3
<400> 19
gatagcagaa acggaaccac                                                 20

<210> 20
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check4
<400> 20
ggtgctgttc acactaccgc                                                 20

<210> 21
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check5
<400> 21
tgacgagatt actgctgctg                                                 20

<210> 22
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ackA-pta-check6
<400> 22
atttccggtt cagatatccg c                                               21

<210> 23
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check1
<400> 23
gggttgacca gcgcaaataa c                                               21

<210> 24
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check2
<400> 24
cagaagtgag taatcttgct tac                                             23

<210> 25
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check3
<400> 25
gatcacttta tcttcgacga tac                                             23

<210> 26
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check4
<400> 26
gcgaacgtgg ataaactgtc tg                                              22

<210> 27
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check5
<400> 27
gctcttaagc accgacgttg ac                                              22

<210> 28
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-adhE-check6
<400> 28
gtcggctcat taacggctat tc                                              22

<210> 29
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-frd-check1
<400> 29
gacggatctc cgccataatc                                                 20

<210> 30
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-frd-check2
<400> 30
tcgccacccg ctactgtatc                                                 20

<210> 31
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-frd-check3
<400> 31
caaagcgttc tgacgaaccg g                                               21

<210> 32
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-frd-check4
<400> 32
tgtgcgatgc acaatatcgt tg                                              22

<210> 33
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-pflB-check1
<400> 33
ttggttgggt tgacatactg g                                               21

<210> 34
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-pflB-check2
<400> 34
tgaacttcat cactgataac c                                               21

<210> 35
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-pflB-check3
<400> 35
ttcaaaggag tgaatgcgac c                                               21

<210> 36
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-pflB-check4
<400> 36
gtcgcggtta tgacaataca gg                                              22

<210> 37
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ldhA-check1
<400> 37
taccgtgccg acgttcaata ac                                              22

<210> 38
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ldhA-check2
<400> 38
catcagcagg cttagcgcaa c                                               21

<210> 39
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ldhA-check3
<400> 39
acctttacgc gtaatgcgtg                                                 20

<210> 40
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-ldhA-check4
<400> 40
accgtttacg ctttccagca c                                               21

<210> 41
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-csc-check1
<400> 41
cgaattatcg atctcgctca ac                                              22

<210> 42
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-csc-check2
<400> 42
cgtctatatt gctgaaggta cag                                             23

<210> 43
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-csc-check3
<400> 43
tcgaaggtcc attcacgcaa c                                               21

<210> 44
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer P395-csc-check4
<400> 44
gattcccacc gcaacgttag                                                 20

<210> 45
<211> 894
<212> DNA
<213> Escherichia coli
<220>
<223> argP
<400> 45
atgaaacgcc cggactacag aacattacag gcactggatg cggtgatacg tgaacgagga     60
tttgagcgcg cggcacaaaa gctgtgcatt acacaatcag ccgtctcaca gcgcattaag    120
caactggaaa atatgttcgg gcagccgctg ttggtgcgta ccgtaccgcc gcgcccgacg    180
gaacaagggc aaaaactgct ggcactgctg cgccaggtgg agttgctgga agaagagtgg    240
ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt cactggcggt caacgccgac    300
agtctggcga cgtggttgct tcctgcactg gctcctgtgt tggctgattc gcctatccgc    360
ctcaacttgc aggtagaaga tgaaacccgc actcaggaac gtctgcgccg cggcgaagtg    420
gtcggcgcgg tgagtattca acatcaggcg ctgccgagtt gtcttgtcga taaacttggt    480
gcgctcgact atctgttcgt cagctcaaaa ccctttgccg aaaaatattt ccctaacggc    540
gtaacgcgtt cggcattact gaaagcgcca gtggtcgcgt ttgaccatct tgacgatatg    600
caccaggcct ttttgcagca aaacttcgat ctgcctccag gcagcgtgcc ctgccatatc    660
gttaattctt cagaagcgtt cgtacaactt gctcgccagg gcaccacctg ctgtatgatc    720
ccgcacctgc aaatcgagaa agaactggcc agcggtgaac tgattgactt aacgccgggg    780
ctatttcaac gccggatgct ctactggcac cgctttgctc ctgaaagccg catgatgcgt    840
aaagtcactg atgcgttgct cgattatggt cacaaagtcc ttcgtcagga ttaa          894

<210> 46
<211> 297
<212> PRT
<213> Escherichia coli
<220>
<223> argP
<400> 46
Met Lys Arg Pro Asp Tyr Arg Thr Leu Gln Ala Leu Asp Ala Val Ile
1               5                   10                  15
Arg Glu Arg Gly Phe Glu Arg Ala Ala Gln Lys Leu Cys Ile Thr Gln
            20                  25                  30
Ser Ala Val Ser Gln Arg Ile Lys Gln Leu Glu Asn Met Phe Gly Gln
        35                  40                  45
Pro Leu Leu Val Arg Thr Val Pro Pro Arg Pro Thr Glu Gln Gly Gln
    50                  55                  60
Lys Leu Leu Ala Leu Leu Arg Gln Val Glu Leu Leu Glu Glu Glu Trp
65                  70                  75                  80
Leu Gly Asp Glu Gln Thr Gly Ser Thr Pro Leu Leu Leu Ser Leu Ala
                85                  90                  95
Val Asn Ala Asp Ser Leu Ala Thr Trp Leu Leu Pro Ala Leu Ala Pro
            100                 105                 110
Val Leu Ala Asp Ser Pro Ile Arg Leu Asn Leu Gln Val Glu Asp Glu
        115                 120                 125
Thr Arg Thr Gln Glu Arg Leu Arg Arg Gly Glu Val Val Gly Ala Val
    130                 135                 140
Ser Ile Gln His Gln Ala Leu Pro Ser Cys Leu Val Asp Lys Leu Gly
145                 150                 155                 160
Ala Leu Asp Tyr Leu Phe Val Ser Ser Lys Pro Phe Ala Glu Lys Tyr
                165                 170                 175
Phe Pro Asn Gly Val Thr Arg Ser Ala Leu Leu Lys Ala Pro Val Val
            180                 185                 190
Ala Phe Asp His Leu Asp Asp Met His Gln Ala Phe Leu Gln Gln Asn
        195                 200                 205
Phe Asp Leu Pro Pro Gly Ser Val Pro Cys His Ile Val Asn Ser Ser
    210                 215                 220
Glu Ala Phe Val Gln Leu Ala Arg Gln Gly Thr Thr Cys Cys Met Ile
225                 230                 235                 240
Pro His Leu Gln Ile Glu Lys Glu Leu Ala Ser Gly Glu Leu Ile Asp
                245                 250                 255
Leu Thr Pro Gly Leu Phe Gln Arg Arg Met Leu Tyr Trp His Arg Phe
            260                 265                 270
Ala Pro Glu Ser Arg Met Met Arg Lys Val Thr Asp Ala Leu Leu Asp
        275                 280                 285
Tyr Gly His Lys Val Leu Arg Gln Asp
    290                 295

<210> 47
<211> 894
<212> DNA
<213> Escherichia coli
<220>
<223> argP Mut
<400> 47
atgaaacgcc cggactacag aacattacag gcactggatg cggtgatacg tgaacgagga     60
tttgagcgcg cggcacaaaa gctgtgcatt acacaatcag ccgtctcaca gcgcattaag    120
caactggaaa atatgttcgg gcagccgctg ttggtgcgta ccgtaccgcc gcgcccgacg    180
gaacaagggc aaaaactgct ggcactgctg cgccaggtgg agttgctgga agaagagtgg    240
ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt cactggaggt caacgccgac    300
agtctggcga cgtggttgct tcctgcactg gctcctgtgt tggctgattc gcctatccgc    360
ctcaacttgc aggtagaaga tgaaacccgc actcaggaac gtctgcgccg cggcgaagtg    420
gtcggcgcgg tgagtattca acatcaggcg ctgccgagtt gtcttgtcga taaacttggt    480
gcgctcgact atctgttcgt cagctcaaaa ccctttgccg aaaaatattt ccctaacggc    540
gtaacgcgtt cggcattact gaaagcgcca gtggtcgcgt ttgaccatct tgacgatatg    600
caccaggcct ttttgcagca aaacttcgat ctgcctccag gcagcgtgcc ctgccatatc    660
gttaattctt cagaagcgtt cgtacaactt gctcgccagg gcaccacctg ctgtatgatc    720
ccgcacctgc aaatcgagaa agaactggcc agcggtgaac tgattgactt aacgccgggg    780
ctatttcaac gccggatgct ctactggcac cgctttgctc ctgaaagccg catgatgcgt    840
aaagtcactg atgcgttgct cgattatggt cacaaagtcc ttcgtcagga ttaa          894

<210> 48
<211> 297
<212> PRT
<213> Escherichia coli
<220>
<223> argP Mut
<400> 48
Met Lys Arg Pro Asp Tyr Arg Thr Leu Gln Ala Leu Asp Ala Val Ile
1               5                   10                  15
Arg Glu Arg Gly Phe Glu Arg Ala Ala Gln Lys Leu Cys Ile Thr Gln
            20                  25                  30
Ser Ala Val Ser Gln Arg Ile Lys Gln Leu Glu Asn Met Phe Gly Gln
        35                  40                  45
Pro Leu Leu Val Arg Thr Val Pro Pro Arg Pro Thr Glu Gln Gly Gln
    50                  55                  60
Lys Leu Leu Ala Leu Leu Arg Gln Val Glu Leu Leu Glu Glu Glu Trp
65                  70                  75                  80
Leu Gly Asp Glu Gln Thr Gly Ser Thr Pro Leu Leu Leu Ser Leu Glu
                85                  90                  95
Val Asn Ala Asp Ser Leu Ala Thr Trp Leu Leu Pro Ala Leu Ala Pro
            100                 105                 110
Val Leu Ala Asp Ser Pro Ile Arg Leu Asn Leu Gln Val Glu Asp Glu
        115                 120                 125
Thr Arg Thr Gln Glu Arg Leu Arg Arg Gly Glu Val Val Gly Ala Val
    130                 135                 140
Ser Ile Gln His Gln Ala Leu Pro Ser Cys Leu Val Asp Lys Leu Gly
145                 150                 155                 160
Ala Leu Asp Tyr Leu Phe Val Ser Ser Lys Pro Phe Ala Glu Lys Tyr
                165                 170                 175
Phe Pro Asn Gly Val Thr Arg Ser Ala Leu Leu Lys Ala Pro Val Val
            180                 185                 190
Ala Phe Asp His Leu Asp Asp Met His Gln Ala Phe Leu Gln Gln Asn
        195                 200                 205
Phe Asp Leu Pro Pro Gly Ser Val Pro Cys His Ile Val Asn Ser Ser
    210                 215                 220
Glu Ala Phe Val Gln Leu Ala Arg Gln Gly Thr Thr Cys Cys Met Ile
225                 230                 235                 240
Pro His Leu Gln Ile Glu Lys Glu Leu Ala Ser Gly Glu Leu Ile Asp
                245                 250                 255
Leu Thr Pro Gly Leu Phe Gln Arg Arg Met Leu Tyr Trp His Arg Phe
            260                 265                 270
Ala Pro Glu Ser Arg Met Met Arg Lys Val Thr Asp Ala Leu Leu Asp
        275                 280                 285
Tyr Gly His Lys Val Leu Arg Gln Asp
    290                 295

<210> 49
<211> 1026
<212> DNA
<213> Escherichia coli
<220>
<223> lpxD
<400> 49
atgccttcaa ttcgactggc tgatttagcg cagcagttgg atgcagaact acacggtgat     60
ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg tcacattacg    120
ttcatggtta acccaaaata ccgtgagcat ttaggcttgt gccaggcgtc cgcggttgtc    180
atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc tggtagtgaa gaatccctac    240
ctgacttacg cgcgcatggc gcaaatttta gataccacgc cgcagcccgc gcagaacatt    300
gcacccagtg cggtgatcga cgcgacggcg aagctgggta acaacgtatc gattggcgct    360
aacgcggtga ttgagtccgg cgttgaactg ggcgataacg tgattatcgg tgccggttgc    420
ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa cgtaaccatt    480
taccatgaga tccagatcgg tcagaattgc ctgatccagt ccggaacagt ggtaggcgca    540
gacggctttg gttatgccaa cgatcgtggt aactgggtga agatcccaca gattggtcgc    600
gtaattattg gcgatcgcgt ggagatcggt gcctgcacaa ccatcgatcg cggcgcgctg    660
gatgacacta ttattggcaa tggcgtgatc attgataacc agtgccagat tgcacataac    720
gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg cagcctgaaa    780
attggtcgtt actgcatgat cggcggagcc agcgtaatca acgggcatat ggaaatatgc    840
gacaaagtga cggttacggg catgggtatg gtgatgcgtc ccatcactga accaggcgtc    900
tattcctcag gcattccgct gcaacccaac aaagtctggc gcaaaaccgc tgcactggtg    960
atgaacattg atgacatgag caagcgtctg aaatcgcttg agcgcaaggt taatcaacaa   1020
gactaa                                                              1026

<210> 50
<211> 341
<212> PRT
<213> Escherichia coli
<220>
<223> lpxD
<400> 50
Met Pro Ser Ile Arg Leu Ala Asp Leu Ala Gln Gln Leu Asp Ala Glu
1               5                   10                  15
Leu His Gly Asp Gly Asp Ile Val Ile Thr Gly Val Ala Ser Met Gln
            20                  25                  30
Ser Ala Gln Thr Gly His Ile Thr Phe Met Val Asn Pro Lys Tyr Arg
        35                  40                  45
Glu His Leu Gly Leu Cys Gln Ala Ser Ala Val Val Met Thr Gln Asp
    50                  55                  60
Asp Leu Pro Phe Ala Lys Ser Ala Ala Leu Val Val Lys Asn Pro Tyr
65                  70                  75                  80
Leu Thr Tyr Ala Arg Met Ala Gln Ile Leu Asp Thr Thr Pro Gln Pro
                85                  90                  95
Ala Gln Asn Ile Ala Pro Ser Ala Val Ile Asp Ala Thr Ala Lys Leu
            100                 105                 110
Gly Asn Asn Val Ser Ile Gly Ala Asn Ala Val Ile Glu Ser Gly Val
        115                 120                 125
Glu Leu Gly Asp Asn Val Ile Ile Gly Ala Gly Cys Phe Val Gly Lys
    130                 135                 140
Asn Ser Lys Ile Gly Ala Gly Ser Arg Leu Trp Ala Asn Val Thr Ile
145                 150                 155                 160
Tyr His Glu Ile Gln Ile Gly Gln Asn Cys Leu Ile Gln Ser Gly Thr
                165                 170                 175
Val Val Gly Ala Asp Gly Phe Gly Tyr Ala Asn Asp Arg Gly Asn Trp
            180                 185                 190
Val Lys Ile Pro Gln Ile Gly Arg Val Ile Ile Gly Asp Arg Val Glu
        195                 200                 205
Ile Gly Ala Cys Thr Thr Ile Asp Arg Gly Ala Leu Asp Asp Thr Ile
    210                 215                 220
Ile Gly Asn Gly Val Ile Ile Asp Asn Gln Cys Gln Ile Ala His Asn
225                 230                 235                 240
Val Val Ile Gly Asp Asn Thr Ala Val Ala Gly Gly Val Ile Met Ala
                245                 250                 255
Gly Ser Leu Lys Ile Gly Arg Tyr Cys Met Ile Gly Gly Ala Ser Val
            260                 265                 270
Ile Asn Gly His Met Glu Ile Cys Asp Lys Val Thr Val Thr Gly Met
        275                 280                 285
Gly Met Val Met Arg Pro Ile Thr Glu Pro Gly Val Tyr Ser Ser Gly
    290                 295                 300
Ile Pro Leu Gln Pro Asn Lys Val Trp Arg Lys Thr Ala Ala Leu Val
305                 310                 315                 320
Met Asn Ile Asp Asp Met Ser Lys Arg Leu Lys Ser Leu Glu Arg Lys
                325                 330                 335
Val Asn Gln Gln Asp
            340

<210> 51
<211> 1026
<212> DNA
<213> Escherichia coli
<220>
<223> lpxD Mut
<400> 51
atgccttcaa ttcgactggc tgatttagcg cagcagttgg atacagaact acacggtgat     60
ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg tcacattacg    120
ttcatggtta acccaaaata ccgtgagcat ttaggcttgt gccaggcgtc cgcggttgtc    180
atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc tggtagtgaa gaatccctac    240
ctgacttacg cgcgcatggc gcaaatttta gataccacgc cgcagcccgc gcagaacatt    300
gcacccagtg cggtgatcga cgcgacggcg aagctgggta acaacgtatc gattggcgct    360
aacgcggtga ttgagtccgg cgttgaactg ggcgataacg tgattatcgg tgccggttgc    420
ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa cgtaaccatt    480
taccatgaga tccagatcgg tcagaattgc ctgatccagt ccggaacagt ggtaggcgca    540
gacggctttg gttatgccaa cgatcgtggt aactgggtga agatcccaca gattggtcgc    600
gtaattattg gcgatcgcgt ggagatcggt gcctgcacaa ccatcgatcg cggcgcgctg    660
gatgacacta ttattggcaa tggcgtgatc attgataacc agtgccagat tgcacataac    720
gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg cagcctgaaa    780
attggtcgtt actgcatgat cggcggagcc agcgtaatca acgggcatat ggaaatatgc    840
gacaaagtga cggttacggg catgggtatg gtgatgcgtc ccatcactga accaggcgtc    900
tattcctcag gcattccgct gcaacccaac aaagtctggc gcaaaaccgc tgcactggtg    960
atgaacattg atgacatgag caagcgtctg aaatcgcttg agcgcaaggt taatcaacaa   1020
gactaa                                                              1026

<210> 52
<211> 341
<212> PRT
<213> Escherichia coli
<220>
<223> lpxD Mut
<400> 52
Met Pro Ser Ile Arg Leu Ala Asp Leu Ala Gln Gln Leu Asp Thr Glu
1               5                   10                  15
Leu His Gly Asp Gly Asp Ile Val Ile Thr Gly Val Ala Ser Met Gln
            20                  25                  30
Ser Ala Gln Thr Gly His Ile Thr Phe Met Val Asn Pro Lys Tyr Arg
        35                  40                  45
Glu His Leu Gly Leu Cys Gln Ala Ser Ala Val Val Met Thr Gln Asp
    50                  55                  60
Asp Leu Pro Phe Ala Lys Ser Ala Ala Leu Val Val Lys Asn Pro Tyr
65                  70                  75                  80
Leu Thr Tyr Ala Arg Met Ala Gln Ile Leu Asp Thr Thr Pro Gln Pro
                85                  90                  95
Ala Gln Asn Ile Ala Pro Ser Ala Val Ile Asp Ala Thr Ala Lys Leu
            100                 105                 110
Gly Asn Asn Val Ser Ile Gly Ala Asn Ala Val Ile Glu Ser Gly Val
        115                 120                 125
Glu Leu Gly Asp Asn Val Ile Ile Gly Ala Gly Cys Phe Val Gly Lys
    130                 135                 140
Asn Ser Lys Ile Gly Ala Gly Ser Arg Leu Trp Ala Asn Val Thr Ile
145                 150                 155                 160
Tyr His Glu Ile Gln Ile Gly Gln Asn Cys Leu Ile Gln Ser Gly Thr
                165                 170                 175
Val Val Gly Ala Asp Gly Phe Gly Tyr Ala Asn Asp Arg Gly Asn Trp
            180                 185                 190
Val Lys Ile Pro Gln Ile Gly Arg Val Ile Ile Gly Asp Arg Val Glu
        195                 200                 205
Ile Gly Ala Cys Thr Thr Ile Asp Arg Gly Ala Leu Asp Asp Thr Ile
    210                 215                 220
Ile Gly Asn Gly Val Ile Ile Asp Asn Gln Cys Gln Ile Ala His Asn
225                 230                 235                 240
Val Val Ile Gly Asp Asn Thr Ala Val Ala Gly Gly Val Ile Met Ala
                245                 250                 255
Gly Ser Leu Lys Ile Gly Arg Tyr Cys Met Ile Gly Gly Ala Ser Val
            260                 265                 270
Ile Asn Gly His Met Glu Ile Cys Asp Lys Val Thr Val Thr Gly Met
        275                 280                 285
Gly Met Val Met Arg Pro Ile Thr Glu Pro Gly Val Tyr Ser Ser Gly
    290                 295                 300
Ile Pro Leu Gln Pro Asn Lys Val Trp Arg Lys Thr Ala Ala Leu Val
305                 310                 315                 320
Met Asn Ile Asp Asp Met Ser Lys Arg Leu Lys Ser Leu Glu Arg Lys
                325                 330                 335
Val Asn Gln Gln Asp
            340

<210> 53
<211> 918
<212> DNA
<213> Escherichia coli
<220>
<223> gcvA
<400> 53
atgtctaaac gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat     60
ttaagtttca ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa    120
atcaagtctc ttgaggattt tctggggcta aaactgttcc gccgccgtaa tcgttcactc    180
ctgctgaccg aggaaggtca aagctatttc ctcgatatca aagagatatt ttcgcaatta    240
accgaagcga cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac ggtcagttta    300
ctccccagtt tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat    360
ccgggaattg acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat    420
gttgatgtgg cgatatttta tggtcggggc aactggccgg ggctacgggt ggaaaaactg    480
tacgccgaat atttattgcc ggtgtgttcg ccgctactgc tgacaggcga aaaacccttg    540
aagaccccgg aagatctggc taaacatacg ttattacatg atgcgtcacg ccgtgactgg    600
cagacatata cccgacagtt ggggttaaat catatcaacg ttcagcaagg gccaattttt    660
agtcatagcg ccatggtgct gcaagcggct attcacgggc agggagtggc gctggcaaat    720
aacgtgatgg cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt taatgatgtt    780
ctggtcagta aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga actgggtaaa    840
atagccgcct ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga aaaattccgc    900
tttcgttatg aacaataa                                                  918

<210> 54
<211> 305
<212> PRT
<213> Escherichia coli
<220>
<223> gcvA
<400> 54
Met Ser Lys Arg Leu Pro Pro Leu Asn Ala Leu Arg Val Phe Asp Ala
1               5                   10                  15
Ala Ala Arg His Leu Ser Phe Thr Arg Ala Ala Glu Glu Leu Phe Val
            20                  25                  30
Thr Gln Ala Ala Val Ser His Gln Ile Lys Ser Leu Glu Asp Phe Leu
        35                  40                  45
Gly Leu Lys Leu Phe Arg Arg Arg Asn Arg Ser Leu Leu Leu Thr Glu
    50                  55                  60
Glu Gly Gln Ser Tyr Phe Leu Asp Ile Lys Glu Ile Phe Ser Gln Leu
65                  70                  75                  80
Thr Glu Ala Thr Arg Lys Leu Gln Ala Arg Ser Ala Lys Gly Ala Leu
                85                  90                  95
Thr Val Ser Leu Leu Pro Ser Phe Ala Ile His Trp Leu Val Pro Arg
            100                 105                 110
Leu Ser Ser Phe Asn Ser Ala Tyr Pro Gly Ile Asp Val Arg Ile Gln
        115                 120                 125
Ala Val Asp Arg Gln Glu Asp Lys Leu Ala Asp Asp Val Asp Val Ala
    130                 135                 140
Ile Phe Tyr Gly Arg Gly Asn Trp Pro Gly Leu Arg Val Glu Lys Leu
145                 150                 155                 160
Tyr Ala Glu Tyr Leu Leu Pro Val Cys Ser Pro Leu Leu Leu Thr Gly
                165                 170                 175
Glu Lys Pro Leu Lys Thr Pro Glu Asp Leu Ala Lys His Thr Leu Leu
            180                 185                 190
His Asp Ala Ser Arg Arg Asp Trp Gln Thr Tyr Thr Arg Gln Leu Gly
        195                 200                 205
Leu Asn His Ile Asn Val Gln Gln Gly Pro Ile Phe Ser His Ser Ala
    210                 215                 220
Met Val Leu Gln Ala Ala Ile His Gly Gln Gly Val Ala Leu Ala Asn
225                 230                 235                 240
Asn Val Met Ala Gln Ser Glu Ile Glu Ala Gly Arg Leu Val Cys Pro
                245                 250                 255
Phe Asn Asp Val Leu Val Ser Lys Asn Ala Phe Tyr Leu Val Cys His
            260                 265                 270
Asp Ser Gln Ala Glu Leu Gly Lys Ile Ala Ala Phe Arg Gln Trp Ile
        275                 280                 285
Leu Ala Lys Ala Ala Ala Glu Gln Glu Lys Phe Arg Phe Arg Tyr Glu
    290                 295                 300
Gln
305

<210> 55
<211> 350
<212> DNA
<213> Escherichia coli
<220>
<223> gcvA WT Promoter
<400> 55
ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg     60
gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat    120
cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag    180
aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt    240
tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta    300
cacagtgtga tctaattgtt aaattcattt aacatcaaag tttaaaagcc               350

<210> 56
<211> 349
<212> DNA
<213> Escherichia coli
<220>
<223> gcvA Mut Promoter1
<400> 56
ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg       60
gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat      120
cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag      180
aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt      240
tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaact aataggttac      300
acagtgtgat ctaattgtta aattcattta acatcaaagt ttaaaagcc                  349

<210> 57
<211> 350
<212> DNA
<213> Escherichia coli
<220>
<223> gcvA Mut Promoter2
<400> 57
ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg     60
gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat    120
cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag    180
aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt    240
tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac aaataggtta    300
cacagtgtga tctaattgtt aaattcattt aacatcaaag tttaaaagcc               350

<210> 58
<211> 206
<212> DNA
<213> Escherichia coli
<220>
<223> gcvB
<400> 58
acttcctgag ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa cttttggctt     60
acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac catggtagca    120
aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga ttaatgtagc    180
accgcctaat tgcggtgctt tttttt                                         206

<210> 59
<211> 128
<212> DNA
<213> Escherichia coli
<220>
<223> gcvB WT Promoter
<400> 59
ggcttttaaa ctttgatgtt aaatgaattt aacaattaga tcacactgtg taacctatta     60
gtttttttaa tctgagccat tataaattgt ccgttgagct tctaccagca aatacctata    120
gtggcggc                                                             128

<210> 60
<211> 127
<212> DNA
<213> Escherichia coli
<220>
<223> gcvB Mut1 Promoter
<400> 60
ggcttttaaa ctttgatgtt aaatgaattt aacaattaga tcacactgtg taacctatta     60
gttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag    120
tggcggc                                                              127

<210> 61
<211> 128
<212> DNA
<213> Escherichia coli
<220>
<223> gcvB Mut2 Promoter
<400> 61
ggcttttaaa ctttgatgtt aaatgaattt aacaattaga tcacactgtg taacctattt     60
gtttttttaa tctgagccat tataaattgt ccgttgagct tctaccagca aatacctata    120
gtggcggc                                                             128

<210> 62
<211> 73
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_1_F
<400> 62
ttgctggaag aagagtggct gggcgatgaa caaaccggtt cgactccgct gatatcggaa     60
gccctgggcc aac                                                        73

<210> 63
<211> 75
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_1_R
<400> 63
tcagccaaca caggagccag tgcaggaagc aaccacgtcg ccagactgtc cacctgagac     60
aacttgttac agctc                                                      75

<210> 64
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_2_F
<400> 64
actggatgcg gtgatacgtg aacg                                            24

<210> 65
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_2_R
<400> 65
accactggcg ctttcagtaa tgcc                                            24

<210> 66
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_seq_F
<400> 66
ttaccaggag cagacaacag c                                               21

<210> 67
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_seq_R
<400> 67
ggcagatcga agttttgctg c                                               21

<210> 68
<211> 38
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP-pACYC_F
<400> 68
tatcatcgat aagcttatgt tacccgccga cggcttcg                             38

<210> 69
<211> 40
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP-pACYC_R
<400> 69
aagggcatcg gtcgacgtga ggataacgcc tgatatgtgc                           40

<210> 70
<211> 73
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_1_F
<400> 70
taataggtta cacagtgtga tctaattgtt aaattcattt aacatcaaag gatatcggaa     60
gccctgggcc aac                                                        73

<210> 71
<211> 75
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_1_R
<400> 71
aaactcgtaa ggcatttagc ggtggtaatc gtttagacat ggcttttaaa cacctgagac     60
aacttgttac agctc                                                      75

<210> 72
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_2_F
<400> 72
cgcagaccaa ttgcaaacac                                                 20

<210> 73
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_2_R
<400> 73
ctcgcgcagc agaagagctt                                                 20

<210> 74
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_seq_F
<400> 74
agcagatcaa ccgtactgac                                                 20

<210> 75
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_seq_R
<400> 75
agtttacgcg tcgcttcggt                                                 20

<210> 76
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA-pACYC_F
<400> 76
tatcatcgat aagcttaagt gccgccacta taggtatttg c                         41

<210> 77
<211> 37
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA-pACYC_R
<400> 77
aagggcatcg gtcgactggt catggtcgta ccctacg                              37

<210> 78
<211> 73
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_1_F
<400> 78
tgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt gatatcggaa     60
gccctgggcc aac                                                        73

<210> 79
<211> 75
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_1_R
<400> 79
attataaatt gtccgttgag cttctaccag caaataccta tagtggcggc cacctgagac     60
aacttgttac agctc                                                      75

<210> 80
<211> 25
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_seq_F
<400> 80
gccgcaatta tttctgcctg tatgc                                           25

<210> 81
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_seq_R
<400> 81
cacaaaaagc tcttctgctg cgcg                                            24

<210> 82
<211> 40
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB-pACYC_F
<400> 82
tatcatcgat aagcttggtc gaactggatc agtaattcgc                           40

<210> 83
<211> 40
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB-pACYC_R
<400> 83
aagggcatcg gtcgaccggt ggtaatcgtt tagacatggc                           40

<210> 84
<211> 73
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_1_F
<400> 84
tatcgttatt gttaacgcgg cgcgttctcg tggcgttacc gaagcgcgtc gatatcggaa     60
gccctgggcc aac                                                        73

<210> 85
<211> 75
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_1_R
<400> 85
gaacgtaagc atgcagaata gcagcgccgt ttgcagactg atcgaccagc cacctgagac     60
aacttgttac agctc                                                      75

<210> 86
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_2_F
<400> 86
ggataccgtg ggcaacttcc ttgc                                            24

<210> 87
<211> 25
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_2_R
<400> 87
gttagaaacc accatcgaga agccg                                           25

<210> 88
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_seq_F
<400> 88
cgctgtttat ctacagcctg g                                               21

<210> 89
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Primer brnQ_seq_R
<400> 89
ggataaatag cggtcagcac c                                               21

<210> 90
<211> 77
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_1C_F
<400> 90
catcggtaaa acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatatc     60
ggaagccctg ggccaac                                                    77

<210> 91
<211> 78
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_1C_R
<400> 91
ggtgcagttc tttgcgtggc ccggcgatct tatattgatc gcctaaagtc atccacctga     60
gacaacttgt tacagctc                                                   78

<210> 92
<211> 25
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_fix_F
<400> 92
cgatcaacga atataactcg ctgcg                                           25

<210> 93
<211> 25
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_fix_R
<400> 93
ataataacac ggcctgccgc aatcg                                           25

<210> 94
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_flank_F
<400> 94
atgctgtagg cggtaacgcc at                                              22

<210> 95
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_flank_R
<400> 95
atacgttgtt acccagcttc gc                                              22

<210> 96
<211> 38
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD-pACYC_F
<400> 96
tatcatcgat aagcttaaat ccgttgccaa cagccagg                             38

<210> 97
<211> 36
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD-pACYC_R
<400> 97
aagggcatcg gtcgacaaca cggcctgccg caatcg                               36

<210> 98
<211> 400
<212> DNA
<213> Escherichia coli
<220>
<223> gcvB_KO_Fix
<400> 98
tgccctgagc gcctgttccg gttcgaacta tgtgatgcac accaatgacg gacgtaccat     60
cgtctctgac ggcaaaccac agactgataa cgataccggt atgatttcgt ataaagacgc    120
taatggcaac aaacagcaga tcaaccgtac tgacgtgaaa gagatggtcg aactggatca    180
gtaattcgcg atcgcaaggt gccgccacta taggtatttg ctggtagaag ctcaacggac    240
aatttataat ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa    300
attcatttaa catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt    360
acgagttttt gatgccgcag cacgccattt aagtttcact                          400

<210> 99
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_RT_F
<400> 99
gcccggacta cagaacatta cagg                                            24

<210> 100
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer argP_RT_R
<400> 100
tgagacggct gattgtgtaa tgc                                             23

<210> 101
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_RT_F
<400> 101
ccatttaagt ttcactcgcg cagc                                            24

<210> 102
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvA_RT_R
<400> 102
ggcggcggaa cagttttagc                                                 20

<210> 103
<211> 27
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_RT_F
<400> 103
taggcggtgc tacattaatc actatgg                                         27

<210> 104
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer gcvB_RT_R
<400> 104
tgttgtgttt gcaattggtc tgc                                             23

<210> 105
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_RT_F
<400> 105
gatatcgtca tcaccggcgt tgc                                             23

<210> 106
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Primer lpxD_RT_R
<400> 106
gcacaagcct aaatgctcac gg                                              22

<210> 107
<211> 23
<212> DNA
<213> Artificial sequence
<220>
<223> Primer rrsA_RT_F
<400> 107
ctcttgccat cggatgtgcc cag                                             23

<210> 108
<211> 24
<212> DNA
<213> Artificial sequence
<220>
<223> Primer rrsA_RT_R
<400> 108
ccagtgtggc tggtcatcct ctca                                            24

<210>  109
<211>  450
<212>  DNA
<213>  E.coli
<220>
<223>  ygaW 

<400>  109
atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt       60
tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag      120
tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt      180
atgtaccgtg atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa      240
aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg      300
ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt      360
tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa      420
gtcagccgtt accagcaggt aaaagcctga                                       450

<210>  110
<211>  149
<212>  PRT
<213>  E.coli
<220>
<223>  ygaW

<400>  110
Met Phe Ser Pro Gln Ser Arg Leu Arg His Ala Val Ala Asp Thr Phe 
1               5                   10                  15      
Ala Met Val Val Tyr Cys Ser Val Val Asn Met Cys Ile Glu Val Phe 
            20                  25                  30          
Leu Ser Gly Met Ser Phe Glu Gln Ser Phe Tyr Ser Arg Leu Val Ala 
        35                  40                  45              
Ile Pro Val Asn Ile Leu Ile Ala Trp Pro Tyr Gly Met Tyr Arg Asp 
    50                  55                  60                  
Leu Phe Met Arg Ala Ala Arg Lys Val Ser Pro Ser Gly Trp Ile Lys 
65                  70                  75                  80  
Asn Leu Ala Asp Ile Leu Ala Tyr Val Thr Phe Gln Ser Pro Val Tyr 
                85                  90                  95      
Val Ala Ile Leu Leu Val Val Gly Ala Asp Trp His Gln Ile Met Ala 
            100                 105                 110         
Ala Val Ser Ser Asn Ile Val Val Ser Met Leu Met Gly Ala Val Tyr 
        115                 120                 125             
Gly Tyr Phe Leu Asp Tyr Cys Arg Arg Leu Phe Lys Val Ser Arg Tyr 
    130                 135                 140                 
Gln Gln Val Lys Ala 
145  

<210>  111
<211>  987
<212>  DNA
<213>  E.coli
<220>
<223>  zipA

<400>  111
atgatgcagg atttgcgtct gatattaatc attgttggcg cgatcgccat aatcgcttta       60
ctggtacatg gtttctggac cagccgtaaa gaacgatctt ctatgttccg cgatcggcca      120
ttaaaacgaa tgaagtcaaa acgtgacgac gattcttatg acgaggatgt cgaagatgat      180
gagggcgttg gtgaggttcg tgttcaccgc gtgaatcatg ccccggctaa cgctcaggag      240
catgaggctg ctcgtccgtc gccgcaacac cagtaccaac cgccttatgc gtctgcgcag      300
ccgcgtcaac cggtccagca gccgcctgaa gcgcaggtac cgccgcaaca tgctccgcgt      360
ccagcgcagc cggtgcagca gcctgcctat cagccgcagc ctgaacagcc gttgcagcag      420
ccagtttcgc cacaggtcgc gccagcgccg cagcctgtgc attcagcacc gcaaccggca      480
caacaggctt tccagcctgc agaacccgta gcggcaccac agcctgagcc tgtagcggaa      540
ccggctccag ttatggataa accgaagcgc aaagaagcgg tgattatcat gaacgtcgcg      600
gcgcatcacg gtagcgagct aaacggtgaa ctgcttctta acagcattca acaagcgggc      660
ttcatttttg gcgatatgaa tatttaccat cgtcatctta gcccggatgg cagcggcccg      720
gcgttattca gcctggcgaa tatggtgaaa ccgggaacct ttgatcctga aatgaaggat      780
ttcactactc cgggtgtcac catctttatg caggtaccgt cttacggtga cgagctgcag      840
aacttcaagc tgatgctgca atctgcgcag catattgccg atgaagtggg cggtgtcgtg      900
cttgacgatc agcgccgtat gatgactccg cagaaattgc gcgagtacca ggacatcatc      960
cgcgaagtca aagacgccaa cgcctga                                          987

<210>  112
<211>  328
<212>  PRT
<213>  E.coli
<220>
<223>  zipA

<400>  112
Met Met Gln Asp Leu Arg Leu Ile Leu Ile Ile Val Gly Ala Ile Ala 
1               5                   10                  15      
Ile Ile Ala Leu Leu Val His Gly Phe Trp Thr Ser Arg Lys Glu Arg 
            20                  25                  30          
Ser Ser Met Phe Arg Asp Arg Pro Leu Lys Arg Met Lys Ser Lys Arg 
        35                  40                  45              
Asp Asp Asp Ser Tyr Asp Glu Asp Val Glu Asp Asp Glu Gly Val Gly 
    50                  55                  60                  
Glu Val Arg Val His Arg Val Asn His Ala Pro Ala Asn Ala Gln Glu 
65                  70                  75                  80  
His Glu Ala Ala Arg Pro Ser Pro Gln His Gln Tyr Gln Pro Pro Tyr 
                85                  90                  95      
Ala Ser Ala Gln Pro Arg Gln Pro Val Gln Gln Pro Pro Glu Ala Gln 
            100                 105                 110         
Val Pro Pro Gln His Ala Pro Arg Pro Ala Gln Pro Val Gln Gln Pro 
        115                 120                 125             
Ala Tyr Gln Pro Gln Pro Glu Gln Pro Leu Gln Gln Pro Val Ser Pro 
    130                 135                 140                 
Gln Val Ala Pro Ala Pro Gln Pro Val His Ser Ala Pro Gln Pro Ala 
145                 150                 155                 160 
Gln Gln Ala Phe Gln Pro Ala Glu Pro Val Ala Ala Pro Gln Pro Glu 
                165                 170                 175     
Pro Val Ala Glu Pro Ala Pro Val Met Asp Lys Pro Lys Arg Lys Glu 
            180                 185                 190         
Ala Val Ile Ile Met Asn Val Ala Ala His His Gly Ser Glu Leu Asn 
        195                 200                 205             
Gly Glu Leu Leu Leu Asn Ser Ile Gln Gln Ala Gly Phe Ile Phe Gly 
    210                 215                 220                 
Asp Met Asn Ile Tyr His Arg His Leu Ser Pro Asp Gly Ser Gly Pro 
225                 230                 235                 240 
Ala Leu Phe Ser Leu Ala Asn Met Val Lys Pro Gly Thr Phe Asp Pro 
                245                 250                 255     
Glu Met Lys Asp Phe Thr Thr Pro Gly Val Thr Ile Phe Met Gln Val 
            260                 265                 270         
Pro Ser Tyr Gly Asp Glu Leu Gln Asn Phe Lys Leu Met Leu Gln Ser 
        275                 280                 285             
Ala Gln His Ile Ala Asp Glu Val Gly Gly Val Val Leu Asp Asp Gln 
    290                 295                 300                 
Arg Arg Met Met Thr Pro Gln Lys Leu Arg Glu Tyr Gln Asp Ile Ile 
305                 310                 315                 320 
Arg Glu Val Lys Asp Ala Asn Ala 
                325       

<210>  113
<211>  1425
<212>  DNA
<213>  E.coli
<220>
<223>  lpd

<400>  113
atgagtactg aaatcaaaac tcaggtcgtg gtacttgggg caggccccgc aggttactcc       60
gctgccttcc gttgcgctga tttaggtctg gaaaccgtaa tcgtagaacg ttacaacacc      120
cttggcggtg tttgcctgaa cgtcggctgt atcccttcta aagcactgct gcacgtagca      180
aaagttatcg aagaagccaa agcgctggct gaacacggta tcgtcttcgg cgaaccgaaa      240
accgatattg acaagattcg tacctggaaa gagaaagtga tcaatcagct gaccggtggt      300
ctggctggta tggcgaaagg ccgcaaagtc aaagtggtca acggtctggg taaatttacc      360
ggggctaaca ccctggaagt tgaaggtgag aacggtaaaa ccgtgatcaa cttcgacaac      420
gcgatcattg cagcgggttc tcgcccgatc caactgccgt ttattccgca tgaagatccg      480
cgtatctggg actccactga cgcgctggaa ctgaaagaag taccagaacg cctgctggta      540
atgggtggcg gtatcatcgg tctggaaatg ggcaccgtat accacgcgct gggttcacag      600
attgacgtgg ttgaaatgtt cgaccaggtt atcccggcag ctgacaaaga catcgttaaa      660
gtcttcacca agcgtatcag caagaaattc aacctgatgc tggaaaccaa agttaccgcc      720
gttgaagcga aagaagacgg tatttatgtg acgatggaag gcaaaaaagc acccgctgaa      780
ccgcagcgtt acgacgccgt gctggtagcg attggtcgtg tgccgaacgg taaaaacctc      840
gacgcaggca aagctggcgt ggaagtggac gaccgtggtt tcatccgcgt tgacaaacag      900
ctgcgtacca acgtaccgca catctttgct atcggcgata tcgtcggtca gccgatgctg      960
gcacacaaag gtgttcacga aggtcacgtt gccgctgaag ttatcgccgg taagaaacac     1020
tacttcgatc cgaaagttat cccgtccatc gcctataccg aaccagaagt tgcatgggta     1080
ggtctgactg agaaagaagc gaaagagaaa ggcatcagct atgaaaccgc caccttcccg     1140
tgggctgctt ctggtcgtgc tatcgcttcc gactgcgcag acggtatgac caagctgatt     1200
ttcgacaaag aatctcaccg tgtgatcggt ggtgcgattg tcggtaccaa cggcggcgag     1260
ctgctgggtg aaatcggcct ggcaatcgaa atgggttgtg atgctgaaga catcgcactg     1320
accatccacg cgcacccgac tctgcacgag tctgtgggcc tggcggcaga agtgttcgaa     1380
ggtagcatta ccgacctgcc gaacccgaaa gcgaagaaga agtaa                     1425

<210>  114
<211>  474
<212>  PRT
<213>  E.coli
<220>
<223>  lpd

<400>  114
Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro 
1               5                   10                  15      
Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr 
            20                  25                  30          
Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val 
        35                  40                  45              
Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu 
    50                  55                  60                  
Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys 
65                  70                  75                  80  
Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln 
                85                  90                  95      
Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val 
            100                 105                 110         
Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu 
        115                 120                 125             
Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala 
    130                 135                 140                 
Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro 
145                 150                 155                 160 
Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu 
                165                 170                 175     
Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr 
            180                 185                 190         
Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp 
        195                 200                 205             
Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys 
    210                 215                 220                 
Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala 
225                 230                 235                 240 
Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys 
                245                 250                 255     
Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly 
            260                 265                 270         
Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu 
        275                 280                 285             
Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn 
    290                 295                 300                 
Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu 
305                 310                 315                 320 
Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala 
                325                 330                 335     
Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr 
            340                 345                 350         
Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys 
        355                 360                 365             
Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser 
    370                 375                 380                 
Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile 
385                 390                 395                 400 
Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr 
                405                 410                 415     
Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly 
            420                 425                 430         
Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu 
        435                 440                 445             
His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr 
    450                 455                 460                 
Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys 
465                 470                 

<210>  115
<211>  269
<212>  DNA
<213>  E.coli
<220>
<223>  ldhA mut Promoter

<400>  115
catagtaaat tcccccacca gtttaaccgg cggctgattt tcaaacgcga cgacatccag       60
ttcgctgact gtaagttgtt gccctttcag ctggccttga aatttaactt tttcgccctg      120
ataacgcagt tgctggatat cagaggttaa tgcgagagag agttttccct gccattcctg      180
ccagggagaa aaaatcagtt tatcgatatt gattttgtaa aatattttta gtagcttaaa      240
tgtgattcaa catcactgga gaaagtctt                                        269

<210>  116
<211>  50
<212>  DNA
<213>  E.coli
<220>
<223>  ldhA mut Promoter fragment


<400>  116
tattgatttt gtaaaatatt tttagtagct taaatgtgat tcaacatcac			50

<210>  117
<211>  500
<212>  DNA
<213>  E.coli
<220>
<223>  brnQ_KO_fix

<400>  117
gtgatttctt atcgctatat acctctggtt tttagatccc tccttgcttt aaaacgttat	60
aagcgtttaa attgcgcttc aggtgctgtc atactgactg cattaacgcg gtaaatcgaa      120
aaactattct tcgccgcgcc tggttgggag tatttcccgc taaaattgtt taaatatacc      180
gctgtatcat ccccagggat tggcacaaaa atttaacgtt acaacaccac atccacaggc      240
agtatgattt atcactgaac atttgtttta accacggggc tgcgatgccc cgtggttttt      300
tattgtgttg atgggttagg aattgatgga aagtaagaac aagctaaagc gtgggctaag      360
tacccgccac atacgcttta tggcactggg ttcagcaatt ggcaccgggc tgttttacgg      420
ttcggcagac gccatcaaaa tggccggtcc gagcgtgttg ttggcctata ttatcggtgg      480
tatcgcggcg tatatcatta 						       500
      

<210>  118
<211>  1071
<212>  DNA
<213>  E. coli
<220>
<223>  dadX

<400>  118
atgacccgtc cgatacaggc cagcctcgat ctgcaggcat taaaacagaa tctgtccatt       60
gtccgccagg ccgcgccgca cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg      120
catggtattg agcgtatctg gagcgcgctc ggggccaccg atggctttgc attacttaac      180
ctggaagagg caataacgtt acgtgagcgc ggctggaagg ggccgatcct gatgctggaa      240
ggatttttcc atgctcagga tctggagatt tatgaccagc accgcctgac cacctgcgta      300
cacagcaact ggcagctcaa agcactgcaa aatgcgcggc taaaagcacc gttggatatt      360
tatcttaaag tgaacagtgg gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc      420
gtctggcagc agttgcgggc aatggcgaat gttggcgaga tgaccctgat gtcgcatttt      480
gccgaagcgg aacatcctga tggaatttcc agcgcgatgg cgcgtattga gcaggcggcg      540
gaagggctgg agtgtcggcg ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa      600
gcgcattttg actgggttcg gcctggcatt attttgtatg gcgcttcgcc gtccggtcag      660
tggcgtgata tcgccaatac cggattacgt ccggtgatga cgctaagcag tgagattatt      720
ggtgtccaga cgctaaaagc gggtgagcgt gtgggctacg gcggtcgcta tactgcgcgc      780
gatgaacagc gaatcggcat tgtcgccgca gggtacgccg acggttatcc gcgccacgcg      840
cctaccggta cccctgtttt agtggacggc gtgcgcacca tgacggtggg gaccgtctcg      900
atggatatgc tagcggtcga tttaacgcct tgcccgcagg cggggattgg tacgccggtt      960
gagctgtggg gcaaggagat caaaattgat gatgtcgccg ccgctgccgg aacggtgggc     1020
tatgagttga tgtgcgcgct ggcgttacgc gtcccggttg tgacagtgta a              1071


<210>  119
<211>  356
<212>  PRT
<213>  E. coli
<220>
<223>  dadX

<400>  119

Met Thr Arg Pro Ile Gln Ala Ser Leu Asp Leu Gln Ala Leu Lys Gln 
1               5                   10                  15      
Asn Leu Ser Ile Val Arg Gln Ala Ala Pro His Ala Arg Val Trp Ser 
            20                  25                  30          
Val Val Lys Ala Asn Ala Tyr Gly His Gly Ile Glu Arg Ile Trp Ser 
        35                  40                  45              
Ala Leu Gly Ala Thr Asp Gly Phe Ala Leu Leu Asn Leu Glu Glu Ala 
    50                  55                  60                  
Ile Thr Leu Arg Glu Arg Gly Trp Lys Gly Pro Ile Leu Met Leu Glu 
65                  70                  75                  80  
Gly Phe Phe His Ala Gln Asp Leu Glu Ile Tyr Asp Gln His Arg Leu 
                85                  90                  95      
Thr Thr Cys Val His Ser Asn Trp Gln Leu Lys Ala Leu Gln Asn Ala 
            100                 105                 110         
Arg Leu Lys Ala Pro Leu Asp Ile Tyr Leu Lys Val Asn Ser Gly Met 
        115                 120                 125             
Asn Arg Leu Gly Phe Gln Pro Asp Arg Val Leu Thr Val Trp Gln Gln 
    130                 135                 140                 
Leu Arg Ala Met Ala Asn Val Gly Glu Met Thr Leu Met Ser His Phe 
145                 150                 155                 160 
Ala Glu Ala Glu His Pro Asp Gly Ile Ser Ser Ala Met Ala Arg Ile 
                165                 170                 175     
Glu Gln Ala Ala Glu Gly Leu Glu Cys Arg Arg Ser Leu Ser Asn Ser 
            180                 185                 190         
Ala Ala Thr Leu Trp His Pro Glu Ala His Phe Asp Trp Val Arg Pro 
        195                 200                 205             
Gly Ile Ile Leu Tyr Gly Ala Ser Pro Ser Gly Gln Trp Arg Asp Ile 
    210                 215                 220                 
Ala Asn Thr Gly Leu Arg Pro Val Met Thr Leu Ser Ser Glu Ile Ile 
225                 230                 235                 240 
Gly Val Gln Thr Leu Lys Ala Gly Glu Arg Val Gly Tyr Gly Gly Arg 
                245                 250                 255     
Tyr Thr Ala Arg Asp Glu Gln Arg Ile Gly Ile Val Ala Ala Gly Tyr 
            260                 265                 270         
Ala Asp Gly Tyr Pro Arg His Ala Pro Thr Gly Thr Pro Val Leu Val 
        275                 280                 285             
Asp Gly Val Arg Thr Met Thr Val Gly Thr Val Ser Met Asp Met Leu 
    290                 295                 300                 
Ala Val Asp Leu Thr Pro Cys Pro Gln Ala Gly Ile Gly Thr Pro Val 
305                 310                 315                 320 
Glu Leu Trp Gly Lys Glu Ile Lys Ile Asp Asp Val Ala Ala Ala Ala 
                325                 330                 335     
Gly Thr Val Gly Tyr Glu Leu Met Cys Ala Leu Ala Leu Arg Val Pro 
            340                 345                 350         
Val Val Thr Val 
        355  

<210>  120
<211>  1203
<212>  DNA
<213>  Escherichia coli

<220>
<223>  ackA

<400>  120
atgtcgagta agttagtact ggttctgaac tgcggtagtt cttcactgaa atttgccatc       60
atcgatgcag taaatggtga agagtacctt tctggtttag ccgaatgttt ccacctgccc      120
gaagcacgta tcaaatggaa aatggacggc aataaacagg aagcggcttt aggtgcaggc      180
gccgctcaca gcgaagcgct caactttatc gttaatacta ttctggcaca aaaaccagaa      240
ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac acggcggcga aaagtatacc      300
agctccgtag tgatcgatga gtctgttatt cagggtatca aagatgcagc ttcttttgca      360
ccgctgcaca acccggctca cctgatcggt atcgaagaag ctctgaaatc tttcccacag      420
ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc accagactat gccggaagag      480
tcttacctct acgccctgcc ttacaacctg tacaaagagc acggcatccg tcgttacggc      540
gcgcacggca ccagccactt ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg      600
gtagaagaac tgaacatcat cacctgccac ctgggcaacg gtggttccgt ttctgctatc      660
cgcaacggta aatgcgttga cacctctatg ggcctgaccc cgctggaagg tctggtcatg      720
ggtacccgtt ctggtgatat cgatccggcg atcatcttcc acctgcacga caccctgggc      780
atgagcgttg acgcaatcaa caaactgctg accaaagagt ctggcctgct gggtctgacc      840
gaagtgacca gcgactgccg ctatgttgaa gacaactacg cgacgaaaga agacgcgaag      900
cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca tcggtgccta cactgcgctg      960
atggatggtc gtctggacgc tgttgtattc actggtggta tcggtgaaaa tgccgcaatg     1020
gttcgtgaac tgtctctggg caaactgggc gtgctgggct ttgaagttga tcatgaacgc     1080
aacctggctg cacgtttcgg caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg     1140
gtggttatcc caaccaacga agaactggtt atcgcgcaag acgcgagccg cctgactgcc     1200
tga                                                                   1203

<210>  121
<211>  400
<212>  PRT
<213>  Escherichia coli

<220>
<223>  ackA

<400>  121

Met Ser Ser Lys Leu Val Leu Val Leu Asn Cys Gly Ser Ser Ser Leu 
1               5                   10                  15      
Lys Phe Ala Ile Ile Asp Ala Val Asn Gly Glu Glu Tyr Leu Ser Gly 
            20                  25                  30          
Leu Ala Glu Cys Phe His Leu Pro Glu Ala Arg Ile Lys Trp Lys Met 
        35                  40                  45              
Asp Gly Asn Lys Gln Glu Ala Ala Leu Gly Ala Gly Ala Ala His Ser 
    50                  55                  60                  
Glu Ala Leu Asn Phe Ile Val Asn Thr Ile Leu Ala Gln Lys Pro Glu 
65                  70                  75                  80  
Leu Ser Ala Gln Leu Thr Ala Ile Gly His Arg Ile Val His Gly Gly 
                85                  90                  95      
Glu Lys Tyr Thr Ser Ser Val Val Ile Asp Glu Ser Val Ile Gln Gly 
            100                 105                 110         
Ile Lys Asp Ala Ala Ser Phe Ala Pro Leu His Asn Pro Ala His Leu 
        115                 120                 125             
Ile Gly Ile Glu Glu Ala Leu Lys Ser Phe Pro Gln Leu Lys Asp Lys 
    130                 135                 140                 
Asn Val Ala Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Glu Glu 
145                 150                 155                 160 
Ser Tyr Leu Tyr Ala Leu Pro Tyr Asn Leu Tyr Lys Glu His Gly Ile 
                165                 170                 175     
Arg Arg Tyr Gly Ala His Gly Thr Ser His Phe Tyr Val Thr Gln Glu 
            180                 185                 190         
Ala Ala Lys Met Leu Asn Lys Pro Val Glu Glu Leu Asn Ile Ile Thr 
        195                 200                 205             
Cys His Leu Gly Asn Gly Gly Ser Val Ser Ala Ile Arg Asn Gly Lys 
    210                 215                 220                 
Cys Val Asp Thr Ser Met Gly Leu Thr Pro Leu Glu Gly Leu Val Met 
225                 230                 235                 240 
Gly Thr Arg Ser Gly Asp Ile Asp Pro Ala Ile Ile Phe His Leu His 
                245                 250                 255     
Asp Thr Leu Gly Met Ser Val Asp Ala Ile Asn Lys Leu Leu Thr Lys 
            260                 265                 270         
Glu Ser Gly Leu Leu Gly Leu Thr Glu Val Thr Ser Asp Cys Arg Tyr 
        275                 280                 285             
Val Glu Asp Asn Tyr Ala Thr Lys Glu Asp Ala Lys Arg Ala Met Asp 
    290                 295                 300                 
Val Tyr Cys His Arg Leu Ala Lys Tyr Ile Gly Ala Tyr Thr Ala Leu 
305                 310                 315                 320 
Met Asp Gly Arg Leu Asp Ala Val Val Phe Thr Gly Gly Ile Gly Glu 
                325                 330                 335     
Asn Ala Ala Met Val Arg Glu Leu Ser Leu Gly Lys Leu Gly Val Leu 
            340                 345                 350         
Gly Phe Glu Val Asp His Glu Arg Asn Leu Ala Ala Arg Phe Gly Lys 
        355                 360                 365             
Ser Gly Phe Ile Asn Lys Glu Gly Thr Arg Pro Ala Val Val Ile Pro 
    370                 375                 380                 
Thr Asn Glu Glu Leu Val Ile Ala Gln Asp Ala Ser Arg Leu Thr Ala 
385                 390                 395                 400 


<210> 122
<211> 72
<212> DNA
<213> Artificial sequence
<220>
<223> Primer dadX_KO_F
<400> 122
catggtattg agcgtatctg gagcgcgctc ggggccaccg atggctttgc aattaaccct       60
cactaaaggg cg                                                           72

<210> 123
<211> 73
<212> DNA
<213> Artificial sequence
<220>
<223> Primer dadX_KO_R
<400> 123
accaataccc gcctgcgggc aaggcgttaa atcgaccgct agcatatcca taatacgact       60
cactataggg ctc                                                          73

<210> 124
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer dadX_seq_F
<400> 124
ggctggacga tggcttgcgg                                                   20


<210> 125
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Primer dadX_seq_R
<400> 125
gtgcggtgag ttcaggttcc                                                   20
                
