                         SEQUENCE LISTING

<110>  CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
 
<120>  NEW MOLECULAR ADJUVANT AND VACCINE

<130>  901 474

<150>  EP 15 382 273.9
<151>  2015-05-22

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  positions 700 to 909 of the fabI gene of EColi BL21 (DE3) strain


<220>
<221>  misc_feature
<222>  (1)..(210)

<400>  1
aaaagcttgc cgctccatgc tgaatccggg ttctgccctg ctgacccttt cctaccttgg       60

cgctgagcgc gctatcccga actacaacgt tatgggtctg gcaaaagcgt ctctggaagc      120

gaacgtgcgc tatatggcga acgcgatggg tccggaaggt gtgcgtgtta acgccatctc      180

tgctggtccg atccgtactc tggcggcttc                                       210


<210>  2
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  polypeptide encoded within SEQ ID NO: 1


<220>
<221>  MISC_FEATURE
<222>  (1)..(69)

<400>  2

Lys Ala Cys Arg Ser Met Leu Asn Pro Gly Ser Ala Leu Leu Thr Leu 
1               5                   10                  15      


Ser Tyr Leu Gly Ala Glu Arg Ala Ile Pro Asn Tyr Asn Val Met Gly 
            20                  25                  30          


Leu Ala Lys Ala Ser Leu Glu Ala Asn Val Arg Tyr Met Ala Asn Ala 
        35                  40                  45              


Met Gly Pro Glu Gly Val Arg Val Asn Ala Ile Ser Ala Gly Pro Ile 
    50                  55                  60                  


Arg Thr Leu Ala Ala 
65                  


<210>  3
<211>  780
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fabI gene of EColi BL21 (DE3) strain


<220>
<221>  misc_feature
<222>  (1)..(780)

<400>  3
atgggttttc tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actatccatc       60

gcctacggta tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag      120

aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt      180

ctgcagtgcg atgttgcaga agatgccagc atcgacacca tgttcgctga actggggaaa      240

gtttggccga aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg      300

gatggtgact atgttaacgc cgttacccgt gaaggcttca aaattgccca cgacatcagc      360

tcctacagct tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc      420

ctgctgaccc tttcctacct tggcgctgag cgcgctatcc cgaactacaa cgttatgggt      480

ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa      540

ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta ctctggcggc ttccggtatc      600

aaagacttcc gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt      660

actattgaag atgtgggtaa ctctgcggca ttcctgtgct ccgatctctc tgccggtatc      720

tccggtgaag tagtccacgt tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa      780


<210>  4
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  polypeptide encoded by the fabI gene of EColi BL21 (DE3) strain


<220>
<221>  MISC_FEATURE
<222>  (1)..(262)

<400>  4

Met Gly Phe Leu Ser Gly Lys Arg Ile Leu Val Thr Gly Val Ala Ser 
1               5                   10                  15      


Lys Leu Ser Ile Ala Tyr Gly Ile Ala Gln Ala Met His Arg Glu Gly 
            20                  25                  30          


Ala Glu Leu Ala Phe Thr Tyr Gln Asn Asp Lys Leu Lys Gly Arg Val 
        35                  40                  45              


Glu Glu Phe Ala Ala Gln Leu Gly Ser Asp Ile Val Leu Gln Cys Asp 
    50                  55                  60                  


Val Ala Glu Asp Ala Ser Ile Asp Thr Met Phe Ala Glu Leu Gly Lys 
65                  70                  75                  80  


Val Trp Pro Lys Phe Asp Gly Phe Val His Ser Ile Gly Phe Ala Pro 
                85                  90                  95      


Gly Asp Gln Leu Asp Gly Asp Tyr Val Asn Ala Val Thr Arg Glu Gly 
            100                 105                 110         


Phe Lys Ile Ala His Asp Ile Ser Ser Tyr Ser Phe Val Ala Met Ala 
        115                 120                 125             


Lys Ala Cys Arg Ser Met Leu Asn Pro Gly Ser Ala Leu Leu Thr Leu 
    130                 135                 140                 


Ser Tyr Leu Gly Ala Glu Arg Ala Ile Pro Asn Tyr Asn Val Met Gly 
145                 150                 155                 160 


Leu Ala Lys Ala Ser Leu Glu Ala Asn Val Arg Tyr Met Ala Asn Ala 
                165                 170                 175     


Met Gly Pro Glu Gly Val Arg Val Asn Ala Ile Ser Ala Gly Pro Ile 
            180                 185                 190         


Arg Thr Leu Ala Ala Ser Gly Ile Lys Asp Phe Arg Lys Met Leu Ala 
        195                 200                 205             


His Cys Glu Ala Val Thr Pro Ile Arg Arg Thr Val Thr Ile Glu Asp 
    210                 215                 220                 


Val Gly Asn Ser Ala Ala Phe Leu Cys Ser Asp Leu Ser Ala Gly Ile 
225                 230                 235                 240 


Ser Gly Glu Val Val His Val Asp Gly Gly Phe Ser Ile Ala Ala Met 
                245                 250                 255     


Asn Glu Leu Glu Leu Lys 
            260         


<210>  5
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GenBank: U49695.1


<220>
<221>  misc_feature
<222>  (1)..(942)

<220>
<221>  misc_feature
<222>  (1)..(942)
<223>  LACK gene

<400>  5
accatgaact acgagggtca cctgaagggc caccgcggat gggtcacctc cctggcctgc       60

ccgcagcagg cggggtcgta catcaaggtg gtgtcgacgt cgcgcgatgg cacggccatc      120

tcgtggaaag ccaaccccga ccgccacagc gtggacagcg actacggtct gccgagccac      180

cgcctcgagg gccacaccgg cttcgtgtcg tgtgtgtcgc tggcccacgc caccgactac      240

gcgctgaccg cgtcctggga ccgctccatc cgcatgtggg acctgcgcaa tggccagtgc      300

cagcgcaagt tcctgaagca caccaaggac gtgctcgccg tcgccttctc gccggacgac      360

cgcctgatcg tgtccgcggg ccgcgacaac gtgatccgcg tgtggaacgt ggcgggcgag      420

tgcatgcacg agttcctgcg cgacggccac gaggactggg tgagcagcat ctgtttctcg      480

ccgtcgctgg agcatccgat cgtggtgtcc ggcagctggg acaacaccat caaggtatgg      540

aacgtgaacg ggggcaagtg tgagcgcacg ctcaagggcc acagcaacta cgtgtccacg      600

gtgacggtgt cgccagacgg gtcgctgtgc gcgtccggcg gcaaggacgg cgcggcgctg      660

ctgtgggacc tgagcaccgg cgagcagctg ttcaagatca acgtggagtc gcccatcaac      720

cagatcgcct tctcgcccaa ccgcttctgg atgtgcgtcg cgacggagag gtctctgtcc      780

gtgtacgacc tggagagcaa ggctgtgatt gcggagctga cgccggacgg cgcgaagccg      840

tccgagtgca tctccattgc ctggtccgcc gacggcaaca ctctgtactc cggtcacaag      900

gacaacctga tccgcgtgtg gtccatctcc gacgccgagt aa                         942


<210>  6
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LACK antigen


<220>
<221>  MISC_FEATURE
<222>  (1)..(312)

<400>  6

Met Asn Tyr Glu Gly His Leu Lys Gly His Arg Gly Trp Val Thr Ser 
1               5                   10                  15      


Leu Ala Cys Pro Gln Gln Ala Gly Ser Tyr Ile Lys Val Val Ser Thr 
            20                  25                  30          


Ser Arg Asp Gly Thr Ala Ile Ser Trp Lys Ala Asn Pro Asp Arg His 
        35                  40                  45              


Ser Val Asp Ser Asp Tyr Gly Leu Pro Ser His Arg Leu Glu Gly His 
    50                  55                  60                  


Thr Gly Phe Val Ser Cys Val Ser Leu Ala His Ala Thr Asp Tyr Ala 
65                  70                  75                  80  


Leu Thr Ala Ser Trp Asp Arg Ser Ile Arg Met Trp Asp Leu Arg Asn 
                85                  90                  95      


Gly Gln Cys Gln Arg Lys Phe Leu Lys His Thr Lys Asp Val Leu Ala 
            100                 105                 110         


Val Ala Phe Ser Pro Asp Asp Arg Leu Ile Val Ser Ala Gly Arg Asp 
        115                 120                 125             


Asn Val Ile Arg Val Trp Asn Val Ala Gly Glu Cys Met His Glu Phe 
    130                 135                 140                 


Leu Arg Asp Gly His Glu Asp Trp Val Ser Ser Ile Cys Phe Ser Pro 
145                 150                 155                 160 


Ser Leu Glu His Pro Ile Val Val Ser Gly Ser Trp Asp Asn Thr Ile 
                165                 170                 175     


Lys Val Trp Asn Val Asn Gly Gly Lys Cys Glu Arg Thr Leu Lys Gly 
            180                 185                 190         


His Ser Asn Tyr Val Ser Thr Val Thr Val Ser Pro Asp Gly Ser Leu 
        195                 200                 205             


Cys Ala Ser Gly Gly Lys Asp Gly Ala Ala Leu Leu Trp Asp Leu Ser 
    210                 215                 220                 


Thr Gly Glu Gln Leu Phe Lys Ile Asn Val Glu Ser Pro Ile Asn Gln 
225                 230                 235                 240 


Ile Ala Phe Ser Pro Asn Arg Phe Trp Met Cys Val Ala Thr Glu Arg 
                245                 250                 255     


Ser Leu Ser Val Tyr Asp Leu Glu Ser Lys Ala Val Ile Ala Glu Leu 
            260                 265                 270         


Thr Pro Asp Gly Ala Lys Pro Ser Glu Cys Ile Ser Ile Ala Trp Ser 
        275                 280                 285             


Ala Asp Gly Asn Thr Leu Tyr Ser Gly His Lys Asp Asn Leu Ile Arg 
    290                 295                 300                 


Val Trp Ser Ile Ser Asp Ala Glu 
305                 310         


<210>  7
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter of the FabI gene


<220>
<221>  misc_feature
<222>  (1)..(312)

<400>  7
gtgctggaga atattcggca aggtctgaac cgtcccagcc atcgccatga aagggttagg       60

ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatacgg tcccctcgcc      120

cctctgggga gagggttagg gtgaggggaa aagcgccccc cctgccgcag cctgctccgg      180

tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg tttatctgtt      240

cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata      300

aggattaaag ct                                                          312


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PacI-fabI-promoter-Fw


<220>
<221>  misc_feature
<222>  (1)..(32)

<400>  8
tactggatta attaagtgct ggagaatatt cg                                     32


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PacI-fabI-Rv


<220>
<221>  misc_feature
<222>  (1)..(37)

<400>  9
tactggatta attaattatt tcagttcgag ttcgttc                                37


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PacI-pCIbla-1


<220>
<221>  misc_feature
<222>  (1)..(35)

<400>  10
tactggatta attaaccgcg tatggtgcac tctca                                  35


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PacI-pCIbla-2


<220>
<221>  misc_feature
<222>  (1)..(34)

<400>  11
tactggatta attaacgctg gcaagtgtag cggt                                   34


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BamHI-pCI- bla-1


<220>
<221>  misc_feature
<222>  (1)..(31)

<400>  12
gtacaggatc ccatgtgtca gaggttttca c                                      31


<210>  13
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BamHI-pCI- bla-2


<220>
<221>  misc_feature
<222>  (1)..(32)

<400>  13
gtacaggatc cgcagaagtg gtcctgcaac tt                                     32


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCIseq1


<220>
<221>  misc_feature
<222>  (1)..(21)

<400>  14
tcaatattgg ccattagcca t                                                 21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  106-LACK-pCIseq


<220>
<221>  misc_feature
<222>  (1)..(21)

<400>  15
ccacgagatg gccgtgccat c                                                 21


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LACK-Fw


<220>
<221>  misc_feature
<222>  (1)..(20)

<400>  16
atgaactacg agggtcacct                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LACK-Rv


<220>
<221>  misc_feature
<222>  (1)..(19)

<400>  17
ttactcggcg tcggagatg                                                    19


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCIseq2


<220>
<221>  misc_feature
<222>  (1)..(21)

<400>  18
gttaagggat tttggtcatg a                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCIseq3


<220>
<221>  misc_feature
<222>  (1)..(21)

<400>  19
tcatgaccaa aatcccttaa c                                                 21


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XbaI-pCIseq4


<220>
<221>  misc_feature
<222>  (1)..(18)

<400>  20
tctagagtcg acccgggc                                                     18


<210>  21
<211>  1101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fabI gene and its promoter


<220>
<221>  misc_feature
<222>  (1)..(1101)

<400>  21
gtgctggaga atattcggca aggtctgaac cgtcccagcc atcgccatga aagggttagg       60

ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatacgg tcccctcgcc      120

cctctgggga gagggttagg gtgaggggaa aagcgccccc cctgccgcag cctgctccgg      180

tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg tttatctgtt      240

cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata      300

aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg tgttgccagc      360

aaactatcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc tgaactggca      420

ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc tcaattgggt      480

tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac catgttcgct      540

gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg ttttgcacct      600

ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt caaaattgcc      660

cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc catgctgaat      720

ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat cccgaactac      780

aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg      840

atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg tactctggcg      900

gcttccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt      960

cgccgtaccg ttactattga agatgtgggt aactctgcgg cattcctgtg ctccgatctc     1020

tctgccggta tctccggtga agtagtccac gttgacggcg gtttcagcat cgctgcaatg     1080

aacgaactcg aactgaaata a                                               1101


<210>  22
<211>  5694
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCI-bla-fabI-LACK


<220>
<221>  misc_feature
<222>  (1)..(5694)

<400>  22
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc ctcgagaatt caccatgaac tacgagggtc acctgaaggg ccaccgcgga     1140

tgggtcacct ccctggcctg cccgcagcag gcggggtcgt acatcaaggt ggtgtcgacg     1200

tcgcgcgatg gcacggccat ctcgtggaaa gccaaccccg accgccacag cgtggacagc     1260

gactacggtc tgccgagcca ccgcctcgag ggccacaccg gcttcgtgtc gtgtgtgtcg     1320

ctggcccacg ccaccgacta cgcgctgacc gcgtcctggg accgctccat ccgcatgtgg     1380

gacctgcgca atggccagtg ccagcgcaag ttcctgaagc acaccaagga cgtgctcgcc     1440

gtcgccttct cgccggacga ccgcctgatc gtgtccgcgg gccgcgacaa cgtgatccgc     1500

gtgtggaacg tggcgggcga gtgcatgcac gagttcctgc gcgacggcca cgaggactgg     1560

gtgagcagca tctgtttctc gccgtcgctg gagcatccga tcgtggtgtc cggcagctgg     1620

gacaacacca tcaaggtatg gaacgtgaac gggggcaagt gtgagcgcac gctcaagggc     1680

cacagcaact acgtgtccac ggtgacggtg tcgccagacg ggtcgctgtg cgcgtccggc     1740

ggcaaggacg gcgcggcgct gctgtgggac ctgagcaccg gcgagcagct gttcaagatc     1800

aacgtggagt cgcccatcaa ccagatcgcc ttctcgccca accgcttctg gatgtgcgtc     1860

gcgacggaga ggtctctgtc cgtgtacgac ctggagagca aggctgtgat tgcggagctg     1920

acgccggacg gcgcgaagcc gtccgagtgc atctccattg cctggtccgc cgacggcaac     1980

actctgtact ccggtcacaa ggacaacctg atccgcgtgt ggtccatctc cgacgccgag     2040

taactagagt cgacccgggc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac     2100

atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc     2160

tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa     2220

caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag     2280

gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg     2340

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga     2400

atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg     2460

cagcgtgacc gctacacttg ccagcgttaa ttaagtgctg gagaatattc ggcaaggtct     2520

gaaccgtccc agccatcgcc atgaaagggt taggggctgt atgagcctgt ttgttgctgg     2580

ggtaacaata tttgcacaat acggtcccct cgcccctctg gggagagggt tagggtgagg     2640

ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca     2700

cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc     2760

gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc     2820

cggtaagcgc attctggtaa ccggtgttgc cagcaaacta tccatcgcct acggtatcgc     2880

tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa     2940

aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt     3000

tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt     3060

tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt     3120

taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt     3180

tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc     3240

ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc     3300

tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa     3360

cgccatctct gctggtccga tccgtactct ggcggcttcc ggtatcaaag acttccgcaa     3420

aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt     3480

gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt     3540

ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataattaat     3600

taaccgcgta ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa     3660

gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg     3720

catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac     3780

cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta     3840

atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg     3900

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     3960

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     4020

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     4080

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     4140

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     4200

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag     4260

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     4320

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     4380

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     4440

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     4500

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa     4560

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     4620

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     4680

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     4740

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     4800

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     4860

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat     4920

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg     4980

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc     5040

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg     5100

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag     5160

cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact     5220

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg     5280

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc     5340

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg     5400

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg     5460

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag     5520

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc     5580

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct     5640

ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atct           5694


<210>  23
<211>  4828
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPAL-LACK


<220>
<221>  misc_feature
<222>  (1)..(4828)

<400>  23
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc ctcgagaatt caccatgaac tacgagggtc acctgaaggg ccaccgcgga     1140

tgggtcacct ccctggcctg cccgcagcag gcggggtcgt acatcaaggt ggtgtcgacg     1200

tcgcgcgatg gcacggccat ctcgtggaaa gccaaccccg accgccacag cgtggacagc     1260

gactacggtc tgccgagcca ccgcctcgag ggccacaccg gcttcgtgtc gtgtgtgtcg     1320

ctggcccacg ccaccgacta cgcgctgacc gcgtcctggg accgctccat ccgcatgtgg     1380

gacctgcgca atggccagtg ccagcgcaag ttcctgaagc acaccaagga cgtgctcgcc     1440

gtcgccttct cgccggacga ccgcctgatc gtgtccgcgg gccgcgacaa cgtgatccgc     1500

gtgtggaacg tggcgggcga gtgcatgcac gagttcctgc gcgacggcca cgaggactgg     1560

gtgagcagca tctgtttctc gccgtcgctg gagcatccga tcgtggtgtc cggcagctgg     1620

gacaacacca tcaaggtatg gaacgtgaac gggggcaagt gtgagcgcac gctcaagggc     1680

cacagcaact acgtgtccac ggtgacggtg tcgccagacg ggtcgctgtg cgcgtccggc     1740

ggcaaggacg gcgcggcgct gctgtgggac ctgagcaccg gcgagcagct gttcaagatc     1800

aacgtggagt cgcccatcaa ccagatcgcc ttctcgccca accgcttctg gatgtgcgtc     1860

gcgacggaga ggtctctgtc cgtgtacgac ctggagagca aggctgtgat tgcggagctg     1920

acgccggacg gcgcgaagcc gtccgagtgc atctccattg cctggtccgc cgacggcaac     1980

actctgtact ccggtcacaa ggacaacctg atccgcgtgt ggtccatctc cgacgccgag     2040

taactagagt cgacccgggc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac     2100

atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc     2160

tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa     2220

caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag     2280

gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg     2340

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga     2400

atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg     2460

cagcgtgacc gctacacttg ccagcgttaa ttaagtgctg gagaatattc ggcaaggtct     2520

gaaccgtccc agccatcgcc atgaaagggt taggggctgt atgagcctgt ttgttgctgg     2580

ggtaacaata tttgcacaat acggtcccct cgcccctctg gggagagggt tagggtgagg     2640

ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca     2700

cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc     2760

gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc     2820

cggtaagcgc attctggtaa ccggtgttgc cagcaaacta tccatcgcct acggtatcgc     2880

tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa     2940

aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt     3000

tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt     3060

tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt     3120

taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt     3180

tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc     3240

ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc     3300

tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa     3360

cgccatctct gctggtccga tccgtactct ggcggcttcc ggtatcaaag acttccgcaa     3420

aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt     3480

gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt     3540

ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataattaat     3600

taaccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc     3660

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     3720

ttacagacaa gctgtgaccg tctccgggag ctcatgtgtc agaggttttc acaagttgca     3780

ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc     3840

ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt     3900

atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc     3960

gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat     4020

atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt     4080

tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac     4140

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc     4200

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca     4260

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta     4320

gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct     4380

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg     4440

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc     4500

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta     4560

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg     4620

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt     4680

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg     4740

cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg     4800

ccttttgctc acatggctcg acagatct                                        4828


<210>  24
<211>  3899
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPAL


<220>
<221>  misc_feature
<222>  (1)..(3899)

<400>  24
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcttc     1140

cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca     1200

aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc     1260

tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt     1320

tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa     1380

atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc     1440

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg     1500

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgtta     1560

attaagtgct ggagaatatt cggcaaggtc tgaaccgtcc cagccatcgc catgaaaggg     1620

ttaggggctg tatgagcctg tttgttgctg gggtaacaat atttgcacaa tacggtcccc     1680

tcgcccctct ggggagaggg ttagggtgag gggaaaagcg ccccccctgc cgcagcctgc     1740

tccggtcgga cctggcaact atagctactc acagccaggt tgattataat aaccgtttat     1800

ctgttcgtac tgtttactaa aacgacgaat cgcctgattt tcaggcacaa caagcatcaa     1860

caataaggat taaagctatg ggttttcttt ccggtaagcg cattctggta accggtgttg     1920

ccagcaaact atccatcgcc tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac     1980

tggcattcac ctaccagaac gacaaactga aaggccgcgt agaagaattt gccgctcaat     2040

tgggttctga catcgttctg cagtgcgatg ttgcagaaga tgccagcatc gacaccatgt     2100

tcgctgaact ggggaaagtt tggccgaaat ttgacggttt cgtacactct attggttttg     2160

cacctggcga tcagctggat ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa     2220

ttgcccacga catcagctcc tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc     2280

tgaatccggg ttctgccctg ctgacccttt cctaccttgg cgctgagcgc gctatcccga     2340

actacaacgt tatgggtctg gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga     2400

acgcgatggg tccggaaggt gtgcgtgtta acgccatctc tgctggtccg atccgtactc     2460

tggcggcttc cggtatcaaa gacttccgca aaatgctggc tcattgcgaa gccgttaccc     2520

cgattcgccg taccgttact attgaagatg tgggtaactc tgcggcattc ctgtgctccg     2580

atctctctgc cggtatctcc ggtgaagtag tccacgttga cggcggtttc agcatcgctg     2640

caatgaacga actcgaactg aaataattaa ttaaccgcgt atggtgcact ctcagtacaa     2700

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc     2760

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     2820

gctcatgtgt cagaggtttt cacaagttgc aggaccactt ctgcgctcgg cccttccggc     2880

tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc     2940

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     3000

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     3060

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     3120

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     3180

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     3240

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     3300

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     3360

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa     3420

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     3480

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     3540

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     3600

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     3660

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     3720

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     3780

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     3840

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatct      3899


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leish-1 Forward primer


<220>
<221>  misc_feature
<222>  (1)..(23)

<400>  25
aacttttctg gtcctccggg tag                                               23


<210>  26
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leish-2 Reverse Primer


<220>
<221>  misc_feature
<222>  (1)..(17)

<400>  26
acccccagtt tcccgcc                                                      17


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leish-P Probe


<220>
<221>  misc_feature
<222>  (1)..(18)

<400>  27
aaaaatgggt gcagaaat                                                     18


