                         SEQUENCE LISTING

<110>  Albert Einstein College of Medicine of Yeshiva University
 
<120>  FUSION TAGS FOR PROTEIN EXPRESSION

<130>  09820.037

<150>  62/162,329
<151>  2015-05-15

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FT2: Non-natural green fluorescent protein (GFP) variant.

<400>  1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg cgcggcgagg gcgagggcga tgccaccaac      120

ggcaagctga ccctgaagtt catcagcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccac aggcgtgcag agcttcagcc gctaccccga ccacatgaag      240

cgccacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcagc      300

ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaacttcaa cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa      720


<210>  2
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FT2: Non-natural green fluorescent protein (GFP) variant.

<400>  2

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Ser Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Thr Gly Val Gln Ser Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  3
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sfGFP: Non-natural green fluorescent protein (GFP) variant.

<400>  3

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  4
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCerulean: Non-natural green fluorescent protein (GFP) variant.

<400>  4

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  5
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mVenus: Non-natural green fluorescent protein (GFP) variant.

<400>  5

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Gly Tyr Gly Leu Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Arg 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  6
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBFP2: Non-natural green fluorescent protein (GFP) variant.

<400>  6

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser His Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Val Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Ile Tyr Ile Met Ala Val Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Ser His Tyr Leu Ser Thr Gln Ser Val Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Arg Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  7
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  moxGFP: Non-natural green fluorescent protein (GFP) variant.

<400>  7
atggtgtcca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttctccgtg cggggcgagg gcgagggcga tgccaccaac      120

ggcaagctga ccctgaagtt catcagcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag agcttctccc gctaccccga ccacatgaag      240

cgccacgact tcttcaagag cgccatgccc gaaggctacg tccaggagcg caccatctcc      300

ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaacttcaa ctcccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacgtggagg acggctccgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgtcca cccagtccaa gctgtccaaa gaccccaacg agaagcgcga tcacatggtc      660

cttctggaat tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa      720


<210>  8
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  moxGFP: Non-natural green fluorescent protein (GFP) variant.

<400>  8

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Ser Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Ser Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  9
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  moxCerulean: Non-natural green fluorescent protein (GFP) variant.

<400>  9
atggtgtcaa agggcgaaga gctgtttact ggagtcgtcc caattctggt ggagctggat       60

ggagatgtca acgggcataa gttttcagtg cgaggagagg gagaaggaga cgctaccaac      120

ggcaagctga cactgaaatt catcagcacc acagggaagc tgcccgtgcc ttggccaacc      180

ctggtcacta ccctgagttg gggcgtccag tcatttgccc gatacccaga ccacatgaag      240

cagcatgatt tctttaaatc cgctatgccc gagggctacg tgcaggaacg gacaattttc      300

tttaaggacg atggaacata caaaactaga gcagaggtga agttcgaagg agacactctg      360

gtcaacagga tcgagctgaa gggcattgac tttaaagaag atggaaatat cctgggccac      420

aaactggagt acaacgccat ccatggcaac gtgtacatta cagctgataa gcagaaaaac      480

gggattaagg caaatttcgg actgaactgc aatgtggaag acgggagtgt ccagctggcc      540

gatcactacc agcagaacac tcctatcggc gacgggcccg tgctgctgcc tgataatcat      600

tatctgagca cccagtccaa gctgtctaag gaccccaatg agaaaagaga tcacatggtg      660

ctgctggagt ttgtgaccgc tgctggcatt accctgggga tggacgagct gtacaagtaa      720


<210>  10
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  moxCerulean: Non-natural green fluorescent protein (GFP) variant.

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Ser Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Ser Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Ala Ile His Gly Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Gly Leu Asn Cys Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  11
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  moxVenus: Non-natural green fluorescent protein (GFP) variant.

<400>  11
atggtgtcta aaggcgagga actgttcact ggggtggtgc ccatcctggt ggagctggac       60

ggcgatgtga atgggcacaa gtttagcgtg cgcggagagg gagaagggga cgctaccaac      120

ggaaagctga cactgaaact gatttccacc acaggcaagc tgcccgtgcc ttggccaaca      180

ctggtgacta ccctgggata cggcctccag agcttcgccc ggtatccaga ccacatgaag      240

cagcatgatt tctttaaatc agctatgccc gagggctacg tgcaggaacg gacaatcttc      300

tttaaggacg atgggacata taaaactaga gccgaggtga agtttgaagg ggacactctg      360

gtgaacagga tcgagctgaa gggaattgac ttcaaagaag atggaaatat cctgggccac      420

aagctggagt acaacttcaa cagccataac gtgtatatca ccgccgataa gcagaaaaac      480

ggcatcaagg ctaatttcaa aattaggcac aatgtggaag acggcggggt gcagctggct      540

gatcattacc agcagaacac acctatcggg gacggacccg tgctgctgcc tgataatcac      600

tacctgagct atcagtccaa gctgtctaag gacccaaacg agaaacggga tcatatggtg      660

ctgaaagaat ttgtgactgc cgctgggatt accctgggaa tggatgagct gtataagtga      720


<210>  12
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  moxVenus: Non-natural green fluorescent protein (GFP) variant.

<400>  12

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Leu Ile 
        35                  40                  45              


Ser Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Gly Tyr Gly Leu Gln Ser Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Lys Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  13
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  moxBFP: Non-natural green fluorescent protein (GFP) variant.

<400>  13
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg gcgagggcga tgccaccaac      120

ggcaagctga ccctgaagtt catcagtact accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgagcca cggcgtgcag gtgttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaacttcaa cagccacaac atctatatca tggccgtcaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacagccac      600

tacctgagca cccagtccaa gcttagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tccgcaccgc cgccgggatc actctcggca tggacgagct gtacaag         717


<210>  14
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  moxBFP: Non-natural green fluorescent protein (GFP) variant.

<400>  14

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Ser Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser His Gly Val Gln Val Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Val Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Ile Tyr Ile Met Ala Val Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Ser His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Arg Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  15
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Prolactin signal sequence.

<400>  15

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Cys Gln Gly Val Val Ser Thr Gly 
            20                  25                  30          


Pro Val Ala Thr 
        35      


<210>  16
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  16
gcaatgggcg gtaggcg                                                      17


<210>  17
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  17
gatcgcggcc gcgttacaat tcatccttat taagtttgtg ccc                         43


<210>  18
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  18
gatcaccggt cgtgagcaaa ggagaggaac tgttc                                  35


<210>  19
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  19
gatcgcggcc gcttacagct cgtccttctt atacagctcg tccatccc                    48


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  20
gatcaccggt cgccaccatg gtgtctaaag gcgag                                  35


<210>  21
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  21
gatcgcggcc gcttacagct cgtccttctt atacagctca tccattcc                    48


<210>  22
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  22
gcaatgggcg gtaggcg                                                      17


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  23
gatcgcggcc gcttaattaa gcttgtgccc                                        30


<210>  24
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  24
gatcaccggt cgccaccatg gtgagcaaag gagaggaac                              39


<210>  25
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  25
gatcgcggcc gcttacttgt acagctc                                           27


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  26
gatcaccggt catggtgtct aaaggcgag                                         29


<210>  27
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  27
gatcgcggcc gctcacttat acagctcatc                                        30


<210>  28
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  28
atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgagg                       45


<210>  29
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
ctcgagatct gagtccggac ttgtacagct cgtccatgcc g                           41


<210>  30
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  30
atccgctagc gctaccggtc gccaccatgg tgtcaaaggg cgaagagc                    48


<210>  31
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  31
ctcgagatct gagtccggac ttatacagct cgtccatccc cag                         43


<210>  32
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  32
atccgctagc gctaccggtc gccaccatgg tgtctaaagg cgaggaactg                  50


<210>  33
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  33
ctcgagatct gagtccggac ttatacagct catccattcc caggg                       45


<210>  34
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  34
atccgctagc gctaccggtc gccaccatgt cctcaaaggg agaagaagac aac              53


<210>  35
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTCGAGATCTGAGTCCGGACTTATACAGTTCGTCCATCCCCATCAC

<400>  35
ctcgagatct gagtccggac ttatacagtt cgtccatccc catcac                      46


<210>  36
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  36
gatcaccggt ctacccatac gacgtc                                            26


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  37
gatcaccggt agcgtagtct gggac                                             25


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  38
gatcgcggcc gcttacagct cgtccttctg cttgtcggc                              39


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  39
cggcgtgcag tcgttcgccc gctac                                             25


<210>  40
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  40
gagccacggc gtgcaggcct tcgcccgcta cccc                                   34


<210>  41
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  41
gagccacggc gtgcaggcct tcgcccgcta cccc                                   34


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  42
caggagcgca ccatctcctt caaggacgac ggc                                    33


<210>  43
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  43
gaacggcatc aaggccaact tcaagatccg c                                      31


<210>  44
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  44
ccctgaagtt catcagtact accggcaagc tgccc                                  35


<210>  45
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  45
cggcgtgcag tcgttcagcc gctac                                             25


<210>  46
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  46
cttttgctaa gcctatggct gcaaac                                            26


<210>  47
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  47
gagcacccag tccaagctga gcaaagac                                          28


<210>  48
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  48
gtccttagac agcctggact gatagctc                                          28


<210>  49
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  49
gagctatcag tccaagctgt ctaaggac                                          28


<210>  50
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  50
gatcccatgg gtatggtgag caagggcgag gag                                    33


<210>  51
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  51
gatcccatgg gtatggtgag caaagg                                            26


<210>  52
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  52
gatcccatgg gtatggtgtc taaaggc                                           27


<210>  53
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer.

<400>  53
gatcccatgg gtatgtcctc aaaggg                                            26


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endoplasmic reticulum retention sequence.

<400>  54

Lys Asp Glu Leu 
1               


