                         SEQUENCE LISTING

<110>  GE Healthcare Dharmacon, Inc.
       He, Kaizhang
       Anderson, Emily
       Delaney, Michael
 
<120>  Synthetic Single Guide RNA for Cas9-mediated Gene Editing

<130>  281174-2

<140>  To be determined
<141>  2016-04-07

<150>  US 62/162209
<151>  2015-05-15

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n at positions 1 to 20 is a, g, c or u.

<400>  1
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaga                                   34


<210>  2
<211>  65
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  2
aauagcaagu uaaaauaagg cuaguccguu aucaacuuga aaaaguggca ccgagucggu       60

gcuuu                                                                   65


<210>  3
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to C6-NH2 moiety.

<400>  3
gcugaauuac ucacgcccca guuuuagagc uaga                                   34


<210>  4
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to C6-N3 moiety.

<400>  4
gcugaauuac ucacgcccca guuuuagagc uaga                                   34


<210>  5
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to 
       2'-O-[2-(2-azidoethyl)methylcarbamate]).

<400>  5
gcugaauuac ucacgcccca guuuuagagc uaga                                   34


<210>  6
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to C6-NH2.

<400>  6
guguauuuug accuacgaau guuuuagagc uaga                                   34


<210>  7
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to C6-N3.

<400>  7
guguauuuug accuacgaau guuuuagagc uaga                                   34


<210>  8
<211>  34
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  A at position 34 is linked to 
       2'-O-[2-(2-azidoethyl)methylcarbamate].

<400>  8
guguauuuug accuacgaau guuuuagagc uaga                                   34


<210>  9
<211>  65
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  A at position 1 is linked to hexyne.

<400>  9
aauagcaagu uaaaauaagg cuaguccguu aucaacuuga aaaaguggca ccgagucggu       60

gcuuu                                                                   65


<210>  10
<211>  99
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  A at position 34 is joined via a linker (L) to A at position 35.

<400>  10
guguauuuug accuacgaau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuu                              99


<210>  11
<211>  99
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
guguauuuug accuacgaau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuu                              99


<210>  12
<211>  81
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
guguauuuug accuacgaau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu g                                                 81


<210>  13
<211>  42
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
guguauuuug accuacgaau guuuuagagc uaugcuguuu ug                          42


<210>  14
<211>  74
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
aacagcauag caaguuaaaa uaaggcuagu ccguuaucaa cuugaaaaag uggcaccgag       60

ucggugcuuu uuuu                                                         74


<210>  15
<211>  20
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
guguauuuug accuacgaau                                                   20


